AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG024159


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024159 MtrunA17_Chr8g0386501 98.104 211 4 0 1 211 1 211 5.17e-155 427
MsaG024159 MtrunA17_Chr3g0113051 60.656 183 64 1 1 175 1 183 2.74e-76 228
MsaG024159 MtrunA17_Chr8g0349391 59.538 173 68 1 1 171 1 173 8.41e-74 222
MsaG024159 MtrunA17_Chr7g0247111 53.302 212 95 2 1 208 1 212 8.41e-73 219
MsaG024159 MtrunA17_Chr1g0152431 55.367 177 67 4 1 167 1 175 1.68e-61 190
MsaG024159 MtrunA17_Chr4g0059371 52.000 175 77 2 2 169 3 177 4.95e-61 188
MsaG024159 MtrunA17_Chr4g0059371 50.704 71 35 0 93 163 2 72 3.28e-18 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024159 AT3G61230.1 54.930 213 87 3 2 210 3 210 2.77e-79 236
MsaG024159 AT3G61230.1 53.425 73 34 0 93 165 2 74 1.35e-21 89.0
MsaG024159 AT2G45800.1 56.250 208 85 3 1 202 1 208 1.80e-77 232
MsaG024159 AT2G39900.1 61.538 182 63 2 1 175 1 182 4.22e-77 230
MsaG024159 AT3G55770.3 57.286 199 79 1 1 193 1 199 1.69e-75 226
MsaG024159 AT3G55770.5 57.286 199 79 1 1 193 1 199 1.69e-75 226
MsaG024159 AT3G55770.2 57.286 199 79 1 1 193 1 199 1.69e-75 226
MsaG024159 AT3G55770.1 57.286 199 79 1 1 193 1 199 1.69e-75 226
MsaG024159 AT1G01780.1 53.659 205 88 2 1 203 1 200 3.38e-74 223
MsaG024159 AT3G55770.7 48.927 233 79 2 1 193 1 233 5.59e-68 208
MsaG024159 AT1G10200.1 54.802 177 68 4 1 167 1 175 1.68e-61 190
MsaG024159 AT2G45800.2 54.217 166 70 3 43 202 12 177 3.71e-53 169
MsaG024159 AT2G45800.2 48.438 64 33 0 3 66 68 131 5.50e-15 70.9
MsaG024159 AT3G55770.4 53.906 128 53 1 1 122 1 128 4.31e-42 139
MsaG024159 AT3G55770.4 46.392 97 41 1 95 191 3 88 3.04e-23 91.3
MsaG024159 AT3G55770.6 54.098 122 50 1 1 116 1 122 4.15e-39 131
MsaG024159 AT3G55770.6 46.392 97 41 1 95 191 3 88 2.28e-23 91.3
MsaG024159 AT1G10200.2 51.145 131 54 3 46 167 4 133 1.36e-37 128
MsaG024159 AT1G10200.2 45.455 66 36 0 3 68 61 126 7.70e-15 69.3

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCTATGCTGCTCTTGA+TGG 0.220465 4:-96514411 MsaT024159.1:CDS
ATTACCCGTCTGGGGTTTCT+TGG 0.257758 4:+96515169 None:intergenic
AAACTTTCTGCTTTCTTCTC+AGG 0.328680 4:-96514879 MsaT024159.1:CDS
TTTACCACAAGTCATGCTTT+AGG 0.384207 4:-96514464 MsaT024159.1:CDS
CACAAGCCTTGCATTTCTGT+TGG 0.387121 4:+96515795 None:intergenic
ACAAGCCTTGCATTTCTGTT+GGG 0.412168 4:+96515796 None:intergenic
AAAGGATCTTATTACCCGTC+TGG 0.436417 4:+96515159 None:intergenic
TCTGTCTCTGTGTCTGATGC+TGG 0.438805 4:+96514255 None:intergenic
TCTCAGTTGTTTAAGGCAAA+AGG 0.442224 4:-96514363 MsaT024159.1:CDS
ACCCGTCTGGGGTTTCTTGG+AGG 0.447284 4:+96515172 None:intergenic
AGAAAGCAGAAAGTTTGCTT+GGG 0.452697 4:+96514886 None:intergenic
GAAAGTTTGCTTGGGGCCCT+AGG 0.452962 4:+96514894 None:intergenic
AGCAGGAAAGCCTAGTGAAC+TGG 0.468952 4:-96515027 MsaT024159.1:intron
AACTTTCTGCTTTCTTCTCA+GGG 0.473345 4:-96514878 MsaT024159.1:CDS
TCAATAACCATGTCTTTCAC+TGG 0.477166 4:-96515821 None:intergenic
CAAGAAAACTGTATATCCAT+TGG 0.479463 4:-96514832 MsaT024159.1:CDS
AAGAAAGCAGAAAGTTTGCT+TGG 0.480734 4:+96514885 None:intergenic
AAGGATCTTATTACCCGTCT+GGG 0.489662 4:+96515160 None:intergenic
AACTGTATATCCATTGGAAA+AGG 0.494668 4:-96514826 MsaT024159.1:intron
TCAAGAGCAGCATAGGATGA+AGG 0.519729 4:+96514414 None:intergenic
CTGGGGTTTCTTGGAGGCGA+AGG 0.523891 4:+96515178 None:intergenic
CTTAAACAACTGAGAAAAGT+GGG 0.527258 4:+96514371 None:intergenic
GAATCCATCAAGAGCAGCAT+AGG 0.537698 4:+96514407 None:intergenic
GTTGATACGTTATCTGCTGA+TGG 0.547689 4:-96515755 MsaT024159.1:CDS
AGAATACACACCAGTTCACT+AGG 0.547764 4:+96515017 None:intergenic
CCTTAAACAACTGAGAAAAG+TGG 0.548044 4:+96514370 None:intergenic
TCATGCTTTAGGTGCACACA+TGG 0.562402 4:-96514453 MsaT024159.1:CDS
TACACCTCTTGTGTAACAGC+AGG 0.567186 4:+96514213 None:intergenic
CAAGAGCAGCATAGGATGAA+GGG 0.580724 4:+96514415 None:intergenic
TTGTGATAAAACTGTTCACT+TGG 0.580761 4:-96515777 MsaT024159.1:CDS
GCCTCCAAGAAACCCCAGAC+GGG 0.596184 4:-96515173 MsaT024159.1:intron
TGATCCTGCTGTTACACAAG+AGG 0.603788 4:-96514217 MsaT024159.1:CDS
AGACACAGAGACAGAGACAA+AGG 0.606794 4:-96514247 MsaT024159.1:CDS
CGCCTCCAAGAAACCCCAGA+CGG 0.608247 4:-96515174 MsaT024159.1:CDS
AGTAACTATCAATCAATCGA+AGG 0.610154 4:-96515245 MsaT024159.1:CDS
TTCAGATGTAATCAATGCAA+TGG 0.611041 4:-96515710 MsaT024159.1:CDS
CGTATGTATATACCGCGAGA+AGG 0.635292 4:+96515687 None:intergenic
TTATGACAGTTGACAGTGGA+AGG 0.635630 4:-96514492 MsaT024159.1:intron
GAAAGCAGAAAGTTTGCTTG+GGG 0.651424 4:+96514887 None:intergenic
AGGATCTTATTACCCGTCTG+GGG 0.652203 4:+96515161 None:intergenic
TGGAACCCAACAGAAATGCA+AGG 0.666590 4:-96515801 MsaT024159.1:CDS
TGCTTTAGGTGCACACATGG+AGG 0.676247 4:-96514450 MsaT024159.1:CDS
CTGTTTATGACAGTTGACAG+TGG 0.680767 4:-96514496 MsaT024159.1:intron
TTAAACAACTGAGAAAAGTG+GGG 0.688189 4:+96514372 None:intergenic
TTGGGTTCCAGTGAAAGACA+TGG 0.695332 4:+96515814 None:intergenic
TCAATGCAATGGCCTTCTCG+CGG 0.702963 4:-96515699 MsaT024159.1:intron
TGCACCTAAAGCATGACTTG+TGG 0.763367 4:+96514460 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 96514212 96515834 96514212 ID=MsaG024159
Chr4 mRNA 96514212 96515834 96514212 ID=MsaT024159.1;Parent=MsaG024159
Chr4 exon 96514212 96514505 96514212 ID=MsaT024159.1.exon5;Parent=MsaT024159.1
Chr4 CDS 96514212 96514505 96514212 ID=cds.MsaT024159.1;Parent=MsaT024159.1
Chr4 exon 96514827 96514916 96514827 ID=MsaT024159.1.exon4;Parent=MsaT024159.1
Chr4 CDS 96514827 96514916 96514827 ID=cds.MsaT024159.1;Parent=MsaT024159.1
Chr4 exon 96515028 96515047 96515028 ID=MsaT024159.1.exon3;Parent=MsaT024159.1
Chr4 CDS 96515028 96515047 96515028 ID=cds.MsaT024159.1;Parent=MsaT024159.1
Chr4 exon 96515174 96515270 96515174 ID=MsaT024159.1.exon2;Parent=MsaT024159.1
Chr4 CDS 96515174 96515270 96515174 ID=cds.MsaT024159.1;Parent=MsaT024159.1
Chr4 exon 96515700 96515834 96515700 ID=MsaT024159.1.exon1;Parent=MsaT024159.1
Chr4 CDS 96515700 96515834 96515700 ID=cds.MsaT024159.1;Parent=MsaT024159.1
Gene Sequence

>MsaT024159.1

ATGTCTTTCACTGGAACCCAACAGAAATGCAAGGCTTGTGATAAAACTGTTCACTTGGTTGATACGTTATCTGCTGATGGTAATGTTTTTCACAAGAACTGCTTCAGATGTAATCAATGCAATGGCCTTCTCGCGATGAGTAACTATCAATCAATCGAAGGAGTTTTATACTGCAAGACTCACGCTGAGCAGCTTTTCAAGGATTCCTTCGCCTCCAAGAAACCCCAGACGGCAGGAAAGCCTAGTGAACTGCCTAGGGCCCCAAGCAAACTTTCTGCTTTCTTCTCAGGGACTCAAGACAAATGTTCATCATGCAAGAAAACTGTATATCCATTGGAAAAGTTGACAGTGGAAGGTGAGTTTTACCACAAGTCATGCTTTAGGTGCACACATGGAGGATGTTTTCTCAGCCCTTCATCCTATGCTGCTCTTGATGGATTCATCTACTGCAAACCCCACTTTTCTCAGTTGTTTAAGGCAAAAGGTAGCTATAGTTATCTCTCTAAACAAGCTTCAATAAAGAAAAATGAAGAAATGCAACAAGCTGCAGCAGAGAAAAATGAATCAGCACCAGCATCAGACACAGAGACAGAGACAAAGGAAGAACATGATCCTGCTGTTACACAAGAGGTGTAG

Protein sequence

>MsaT024159.1

MSFTGTQQKCKACDKTVHLVDTLSADGNVFHKNCFRCNQCNGLLAMSNYQSIEGVLYCKTHAEQLFKDSFASKKPQTAGKPSELPRAPSKLSAFFSGTQDKCSSCKKTVYPLEKLTVEGEFYHKSCFRCTHGGCFLSPSSYAALDGFIYCKPHFSQLFKAKGSYSYLSKQASIKKNEEMQQAAAEKNESAPASDTETETKEEHDPAVTQEV*