AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG024228


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024228 MtrunA17_Chr8g0387141 64.780 318 76 5 1 283 1 317 4.08e-126 371
MsaG024228 MtrunA17_Chr1g0186261 65.909 308 73 5 1 283 1 301 2.55e-122 361
MsaG024228 MtrunA17_Chr1g0186281 66.019 309 72 6 1 284 1 301 1.76e-121 359
MsaG024228 MtrunA17_Chr8g0387181 63.694 314 78 7 1 283 1 309 2.50e-117 350
MsaG024228 MtrunA17_Chr1g0186191 58.088 272 66 6 32 284 57 299 2.04e-86 269
MsaG024228 MtrunA17_Chr6g0453251 67.460 126 37 1 1 122 1 126 1.41e-51 165
MsaG024228 MtrunA17_Chr6g0479271 69.167 120 16 3 178 277 7 125 4.41e-44 152
MsaG024228 MtrunA17_Chr8g0387161 62.295 61 12 1 234 283 1 61 2.83e-16 77.4
MsaG024228 MtrunA17_Chr7g0248041 57.377 61 26 0 63 123 1 61 8.86e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024228 AT4G11400.2 26.136 264 150 9 37 272 27 273 1.20e-17 83.2
MsaG024228 AT4G11400.1 26.136 264 150 9 37 272 27 273 1.20e-17 83.2
MsaG024228 AT2G46040.2 27.679 224 146 4 38 248 61 281 7.15e-14 72.0
MsaG024228 AT2G46040.3 27.679 224 146 4 38 248 47 267 7.75e-14 71.6
MsaG024228 AT2G46040.1 27.679 224 146 4 38 248 47 267 7.75e-14 71.6

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCATCACCGTTAAAATTT+TGG 0.110531 4:+97093901 None:intergenic
ATTCTACTTGCAAGCGTAAA+AGG 0.161678 4:-97093812 MsaT024228.1:CDS
TTCTCCCTTGTGAAGGAAAA+AGG 0.214256 4:-97094289 MsaT024228.1:CDS
GGGTTTGGACCTTAATGTTT+TGG 0.293428 4:-97094212 MsaT024228.1:CDS
CATAATTAAGCACATTGATT+TGG 0.300440 4:-97093987 MsaT024228.1:CDS
GGACTCTGTGATAGTTGATC+TGG 0.306502 4:-97094233 MsaT024228.1:CDS
GATTTGAGAATGAGAGGCTT+TGG 0.343884 4:+97094053 None:intergenic
TCACTGAAGCCAAAACATTA+AGG 0.361658 4:+97094203 None:intergenic
CATTATCGGAAATGATAAAC+TGG 0.368928 4:-97093785 MsaT024228.1:CDS
CAAGAGGAGAATTGTATTAG+AGG 0.371040 4:-97094373 MsaT024228.1:CDS
CTCTAATACAATTCTCCTCT+TGG 0.380788 4:+97094374 None:intergenic
TTTACTAAATTTCATGCAAA+TGG 0.388491 4:-97094472 MsaT024228.1:CDS
TTTGATGATGACCCATTGTT+TGG 0.394625 4:+97093648 None:intergenic
TGTGATAGTTGATCTGGGTT+TGG 0.407331 4:-97094227 MsaT024228.1:CDS
TTATACTCCTCAGGATCAAA+TGG 0.411508 4:+97093741 None:intergenic
CCTTCACAAGGGAGAAAAGT+TGG 0.420577 4:+97094296 None:intergenic
TGAGGATCAGCACTATCAAC+AGG 0.430312 4:+97094430 None:intergenic
ATGTGCTTAATTATGTTACT+TGG 0.435716 4:+97093995 None:intergenic
TTTGATCCTGAGGAGTATAA+AGG 0.444102 4:-97093738 MsaT024228.1:CDS
GCGTAAAAGGGAATCATTAT+CGG 0.444431 4:-97093799 MsaT024228.1:CDS
ACACCGAGCATCACCGGCTC+AGG 0.456593 4:+97094340 None:intergenic
CAGCCTCCTTTATACTCCTC+AGG 0.457410 4:+97093732 None:intergenic
AGTCACAAGTTCATGGCAAA+CGG 0.460752 4:-97094526 None:intergenic
CCTCTCATTCTCAAATCTAA+AGG 0.469105 4:-97094047 MsaT024228.1:CDS
TTCTACTTGCAAGCGTAAAA+GGG 0.470153 4:-97093811 MsaT024228.1:CDS
CTGAGGAGTATAAAGGAGGC+TGG 0.479138 4:-97093731 MsaT024228.1:CDS
CAGTATCGAGAAAAGGGTTG+TGG 0.485102 4:-97094255 MsaT024228.1:CDS
GACTCTGTGATAGTTGATCT+GGG 0.491869 4:-97094232 MsaT024228.1:CDS
AGGCCTGAGCCGGTGATGCT+CGG 0.501839 4:-97094343 MsaT024228.1:CDS
GTCACAAGTTCATGGCAAAC+GGG 0.502162 4:-97094525 None:intergenic
TATGCTGCAGTATCGAGAAA+AGG 0.502280 4:-97094262 MsaT024228.1:CDS
TGACTGACACCGAGCATCAC+CGG 0.520466 4:+97094334 None:intergenic
ATCTGAGTGATTTCGAAGGT+TGG 0.523296 4:-97094162 MsaT024228.1:CDS
ATAAATTACCATCACTCTTG+TGG 0.532243 4:+97093960 None:intergenic
AGCATGCAGAACCAAACAAT+GGG 0.540970 4:-97093659 MsaT024228.1:CDS
CCTTTAGATTTGAGAATGAG+AGG 0.541549 4:+97094047 None:intergenic
AGTATCGAGAAAAGGGTTGT+GGG 0.542959 4:-97094254 MsaT024228.1:CDS
ATGCTGCAGTATCGAGAAAA+GGG 0.546828 4:-97094261 MsaT024228.1:CDS
TATACTCCTCAGGATCAAAT+GGG 0.549641 4:+97093742 None:intergenic
AAACAATGGGTCATCATCAA+AGG 0.556398 4:-97093646 MsaT024228.1:CDS
AGGCAATGTGAGGCCTGAGC+CGG 0.560985 4:-97094353 MsaT024228.1:CDS
AGAATGAAATTCAAAAGAAG+CGG 0.567361 4:+97093830 None:intergenic
AAAATTCGGCACTGGTGTCA+AGG 0.572645 4:-97093862 MsaT024228.1:CDS
TGAGGAGTATAAAGGAGGCT+GGG 0.575783 4:-97093730 MsaT024228.1:CDS
TTGGTTAACCACAAGAGTGA+TGG 0.581825 4:-97093968 MsaT024228.1:intron
TTTAGTTTCACGGCGATCAA+GGG 0.587868 4:+97094498 None:intergenic
AAGCATGCAGAACCAAACAA+TGG 0.615428 4:-97093660 MsaT024228.1:CDS
GAAGGCAAGAGATGTTCTGT+CGG 0.647872 4:-97093688 MsaT024228.1:CDS
AATACACCCATTTGATCCTG+AGG 0.649632 4:-97093748 MsaT024228.1:CDS
AAATGGCGATTGTCTTGTCG+AGG 0.655256 4:-97094455 MsaT024228.1:CDS
GATCCTGAGGAGTATAAAGG+AGG 0.656179 4:-97093735 MsaT024228.1:CDS
AAATATCTGAGTGATTTCGA+AGG 0.658560 4:-97094166 MsaT024228.1:CDS
ATTGTATTAGAGGCAATGTG+AGG 0.692266 4:-97094363 MsaT024228.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 97093641 97094536 97093641 ID=MsaG024228
Chr4 mRNA 97093641 97094536 97093641 ID=MsaT024228.1;Parent=MsaG024228
Chr4 exon 97093641 97093927 97093641 ID=MsaT024228.1.exon2;Parent=MsaT024228.1
Chr4 CDS 97093641 97093927 97093641 ID=cds.MsaT024228.1;Parent=MsaT024228.1
Chr4 exon 97093969 97094536 97093969 ID=MsaT024228.1.exon1;Parent=MsaT024228.1
Chr4 CDS 97093969 97094536 97093969 ID=cds.MsaT024228.1;Parent=MsaT024228.1
Gene Sequence

>MsaT024228.1

ATGGCAAACGGGTCTTCCCTTGATCGCCGTGAAACTAAAACTTTTACTAAATTTCATGCAAATGGCGATTGTCTTGTCGAGGATCCTGTTGATAGTGCTGATCCTCAAAAATCTATGTTTTGTCAATTTTTTTCGGTTTACCAAGAGGAGAATTGTATTAGAGGCAATGTGAGGCCTGAGCCGGTGATGCTCGGTGTCAGTCAATTGCTTGATTTATACCAACTTTTCTCCCTTGTGAAGGAAAAAGGAGGGTATGCTGCAGTATCGAGAAAAGGGTTGTGGGACTCTGTGATAGTTGATCTGGGTTTGGACCTTAATGTTTTGGCTTCAGTGAAATTAGTTTATGAAAAATATCTGAGTGATTTCGAAGGTTGGCTCAGCAAAACGTTCGAAGAAAAGAGTTTCAAAAATTGGAATGGTGGGTGTTTGAATTCATTGCCAATAGATCTAGAAAAAGAGCTCCAAAGCCTCTCATTCTCAAATCTAAAGGACAAAGATGATCATTTTGTCACGTTAGAGCCAAGTAACATAATTAAGCACATTGATTTGGTTAACCACAAGAGTGATGGTGTCCAAAATTTTAACGGTGATGGTAATGAGGATGACAATGAAAAATTCGGCACTGGTGTCAAGGATGATCTAGCCGCTTCTTTTGAATTTCATTCTACTTGCAAGCGTAAAAGGGAATCATTATCGGAAATGATAAACTGGATGAAGAATATCGCAATACACCCATTTGATCCTGAGGAGTATAAAGGAGGCTGGGACTTTTTTGTTCAGATGCTGAAGGCAAGAGATGTTCTGTCGGTCAGAAAGCATGCAGAACCAAACAATGGGTCATCATCAAAGGTATAA

Protein sequence

>MsaT024228.1

MANGSSLDRRETKTFTKFHANGDCLVEDPVDSADPQKSMFCQFFSVYQEENCIRGNVRPEPVMLGVSQLLDLYQLFSLVKEKGGYAAVSRKGLWDSVIVDLGLDLNVLASVKLVYEKYLSDFEGWLSKTFEEKSFKNWNGGCLNSLPIDLEKELQSLSFSNLKDKDDHFVTLEPSNIIKHIDLVNHKSDGVQNFNGDGNEDDNEKFGTGVKDDLAASFEFHSTCKRKRESLSEMINWMKNIAIHPFDPEEYKGGWDFFVQMLKARDVLSVRKHAEPNNGSSSKV*