AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG024301

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024301 MtrunA17_Chr8g0387981 98.061 361 7 0 1 361 1 361 0.0 738
MsaG024301 MtrunA17_Chr7g0275531 36.927 371 180 11 1 361 1 327 3.60e-57 189
MsaG024301 MtrunA17_Chr3g0126391 38.564 376 163 17 12 361 7 340 3.15e-56 187
MsaG024301 MtrunA17_Chr8g0347251 35.849 371 187 14 1 349 1 342 2.38e-51 174
MsaG024301 MtrunA17_Chr7g0244981 32.178 202 105 5 1 191 1 181 6.97e-24 99.8
MsaG024301 MtrunA17_Chr8g0334541 33.511 188 99 5 13 198 6 169 1.10e-22 96.3
MsaG024301 MtrunA17_Chr2g0315381 30.583 206 108 6 8 191 11 203 2.84e-22 95.9
MsaG024301 MtrunA17_Chr7g0244971 32.143 196 95 5 9 191 12 182 2.43e-20 90.1
MsaG024301 MtrunA17_Chr2g0315361 29.327 208 115 6 6 191 10 207 5.45e-20 89.4
MsaG024301 MtrunA17_Chr2g0315371 30.144 209 119 5 1 191 1 200 4.56e-19 86.7
MsaG024301 MtrunA17_Chr8g0350621 28.718 195 101 5 13 196 8 175 6.08e-19 85.5
MsaG024301 MtrunA17_Chr5g0428351 30.055 183 100 5 9 191 88 242 1.60e-17 82.4
MsaG024301 MtrunA17_Chr3g0128671 31.694 183 97 5 18 191 23 186 2.36e-17 81.6
MsaG024301 MtrunA17_Chr7g0238921 32.000 150 90 5 45 189 36 178 9.54e-16 77.0
MsaG024301 MtrunA17_Chr2g0284321 33.571 140 84 4 106 244 9 140 1.18e-15 75.1
MsaG024301 MtrunA17_Chr8g0348311 41.860 86 42 2 105 190 134 211 1.74e-15 76.6
MsaG024301 MtrunA17_Chr3g0079691 51.351 74 28 3 120 189 264 333 4.63e-15 76.6
MsaG024301 MtrunA17_Chr4g0051881 53.521 71 25 2 132 197 178 245 5.28e-15 75.1
MsaG024301 MtrunA17_Chr8g0383351 52.308 65 28 1 132 196 160 221 5.59e-15 73.6
MsaG024301 MtrunA17_Chr7g0248691 42.353 85 41 2 105 189 133 209 1.23e-14 73.9
MsaG024301 MtrunA17_Chr5g0418781 43.750 96 50 2 95 189 86 178 1.81e-14 72.8
MsaG024301 MtrunA17_Chr3g0129361 51.471 68 30 1 132 199 144 208 2.00e-14 71.6
MsaG024301 MtrunA17_Chr7g0270191 57.627 59 22 1 132 190 145 200 2.02e-14 73.2
MsaG024301 MtrunA17_Chr3g0139021 44.872 78 40 2 118 194 299 374 2.81e-14 73.6
MsaG024301 MtrunA17_Chr7g0270051 33.775 151 88 3 106 246 24 172 2.93e-14 72.0
MsaG024301 MtrunA17_Chr5g0431811 50.000 62 31 0 130 191 670 731 2.98e-14 74.3
MsaG024301 MtrunA17_Chr7g0228941 47.887 71 34 2 120 189 39 107 3.73e-14 68.6
MsaG024301 MtrunA17_Chr5g0443131 55.172 58 23 1 132 189 157 211 4.61e-14 72.0
MsaG024301 MtrunA17_Chr5g0401821 43.529 85 43 3 106 189 174 254 4.75e-14 71.6
MsaG024301 MtrunA17_Chr1g0194481 42.222 90 44 2 101 190 132 213 4.76e-14 72.4
MsaG024301 MtrunA17_Chr7g0218081 44.048 84 39 2 106 189 389 464 6.67e-14 72.8
MsaG024301 MtrunA17_Chr7g0218081 49.206 63 28 2 127 189 223 281 1.63e-13 71.6
MsaG024301 MtrunA17_Chr7g0263531 46.575 73 35 2 117 189 174 242 8.04e-14 72.4
MsaG024301 MtrunA17_Chr7g0263531 50.704 71 29 2 119 189 351 415 1.81e-13 71.2
MsaG024301 MtrunA17_Chr1g0147881 32.787 122 68 4 71 189 207 317 1.37e-13 70.9
MsaG024301 MtrunA17_Chr8g0379461 39.474 114 61 3 82 189 20 131 1.41e-13 69.7
MsaG024301 MtrunA17_Chr1g0202461 38.144 97 50 3 96 189 227 316 1.65e-13 71.2
MsaG024301 MtrunA17_Chr7g0249311 55.172 58 22 2 132 189 179 232 2.26e-13 71.2
MsaG024301 MtrunA17_Chr7g0224801 54.237 59 24 1 132 190 93 148 3.72e-13 67.0
MsaG024301 MtrunA17_Chr2g0294231 52.542 59 25 1 132 190 203 258 4.57e-13 69.7
MsaG024301 MtrunA17_Chr4g0042831 47.619 63 30 1 127 189 140 199 5.73e-13 69.3
MsaG024301 MtrunA17_Chr4g0042831 53.448 58 24 1 132 189 278 332 3.30e-12 67.0
MsaG024301 MtrunA17_Chr4g0060271 34.694 147 71 6 57 189 175 310 5.85e-13 70.1
MsaG024301 MtrunA17_Chr7g0270831 49.315 73 34 2 118 189 201 271 1.02e-12 68.9
MsaG024301 MtrunA17_Chr8g0335811 40.000 90 46 2 100 189 127 208 1.10e-12 67.0
MsaG024301 MtrunA17_Chr8g0387501 43.077 65 35 1 125 189 122 184 1.26e-12 67.4
MsaG024301 MtrunA17_Chr7g0248721 41.121 107 43 6 94 189 97 194 1.41e-12 66.2
MsaG024301 MtrunA17_Chr1g0184691 49.123 57 27 1 133 189 294 348 1.50e-12 68.2
MsaG024301 MtrunA17_Chr3g0099641 35.484 124 62 5 77 200 207 312 1.52e-12 68.6
MsaG024301 MtrunA17_Chr4g0053281 47.368 57 28 1 133 189 267 321 1.58e-12 67.8
MsaG024301 MtrunA17_Chr7g0243891 55.172 58 22 2 132 189 216 269 2.31e-12 68.2
MsaG024301 MtrunA17_Chr4g0004911 32.857 140 77 4 132 263 184 314 2.34e-12 68.2
MsaG024301 MtrunA17_Chr4g0004911 48.276 58 27 1 132 189 336 390 7.07e-11 63.5
MsaG024301 MtrunA17_Chr5g0418191 51.724 58 25 1 132 189 320 374 2.43e-12 67.8
MsaG024301 MtrunA17_Chr5g0418191 49.206 63 28 2 127 189 146 204 9.60e-12 65.9
MsaG024301 MtrunA17_Chr5g0432161 51.724 58 24 2 132 189 244 297 3.88e-12 67.4
MsaG024301 MtrunA17_Chr5g0432161 50.000 58 26 1 132 189 421 475 9.35e-11 63.2
MsaG024301 MtrunA17_Chr5g0445311 50.000 58 26 1 132 189 364 418 3.93e-12 67.4
MsaG024301 MtrunA17_Chr5g0445311 49.254 67 28 3 125 189 191 253 3.36e-11 64.7
MsaG024301 MtrunA17_Chr2g0288871 48.000 75 36 2 116 189 288 360 3.93e-12 67.4
MsaG024301 MtrunA17_Chr2g0319961 33.051 118 69 2 72 189 28 135 4.16e-12 65.9
MsaG024301 MtrunA17_Chr7g0249301 46.970 66 31 2 132 197 62 123 4.60e-12 66.6
MsaG024301 MtrunA17_Chr1g0150281 50.877 57 26 1 133 189 141 195 5.29e-12 65.9
MsaG024301 MtrunA17_Chr3g0106491 51.724 58 24 2 132 189 198 251 5.49e-12 67.0
MsaG024301 MtrunA17_Chr3g0106491 49.153 59 27 1 132 190 365 420 5.93e-11 63.5
MsaG024301 MtrunA17_Chr8g0386241 37.647 85 45 2 105 189 81 157 5.77e-12 63.9
MsaG024301 MtrunA17_Chr2g0322391 46.835 79 39 2 112 189 328 404 6.38e-12 67.0
MsaG024301 MtrunA17_Chr2g0333851 47.059 68 32 2 122 189 161 224 6.46e-12 65.9
MsaG024301 MtrunA17_Chr4g0071521 40.449 89 48 3 106 193 220 304 6.51e-12 65.9
MsaG024301 MtrunA17_Chr3g0101131 50.000 58 26 1 132 189 415 469 6.75e-12 66.6
MsaG024301 MtrunA17_Chr2g0293481 50.877 57 26 1 133 189 268 322 1.64e-11 65.5
MsaG024301 MtrunA17_Chr1g0188941 50.000 58 26 1 132 189 51 105 1.79e-11 60.8
MsaG024301 MtrunA17_Chr7g0216891 45.205 73 37 2 118 189 204 274 2.05e-11 65.1
MsaG024301 MtrunA17_Chr3g0136961 41.250 80 43 3 111 189 117 193 2.43e-11 63.9
MsaG024301 MtrunA17_Chr1g0189391 46.575 73 32 3 119 190 87 153 3.26e-11 63.9
MsaG024301 MtrunA17_Chr4g0068541 48.276 58 27 1 132 189 208 262 4.35e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024301 AT4G11070.1 39.669 363 143 12 6 353 5 306 2.71e-66 212
MsaG024301 AT4G23810.1 44.912 285 119 7 5 259 7 283 1.16e-64 208
MsaG024301 AT4G11070.2 39.045 356 123 11 6 353 5 274 4.50e-63 203
MsaG024301 AT5G24110.1 39.234 209 93 7 7 205 10 194 6.40e-36 133
MsaG024301 AT2G46400.1 32.534 292 154 8 8 295 4 256 8.87e-35 129
MsaG024301 AT3G56400.1 36.464 181 102 5 12 191 11 179 2.64e-25 104
MsaG024301 AT2G40740.1 58.065 62 26 0 130 191 171 232 3.22e-20 90.1
MsaG024301 AT2G40740.3 58.065 62 26 0 130 191 180 241 4.05e-20 89.7
MsaG024301 AT2G40750.1 33.333 171 96 3 40 201 63 224 9.37e-20 89.7
MsaG024301 AT1G66550.2 35.862 145 81 4 54 191 23 162 1.20e-17 82.0
MsaG024301 AT1G66550.1 51.471 68 32 1 124 191 101 167 3.63e-17 80.5
MsaG024301 AT2G37260.3 36.567 134 72 3 61 189 198 323 3.11e-16 79.3
MsaG024301 AT2G37260.2 36.567 134 72 3 61 189 196 321 3.14e-16 79.3
MsaG024301 AT5G45260.1 57.895 57 24 0 133 189 1209 1265 3.68e-16 80.5
MsaG024301 AT2G37260.1 36.567 134 72 3 61 189 278 403 3.76e-16 79.7
MsaG024301 AT5G52830.1 46.512 86 39 2 105 190 143 221 1.26e-15 77.4
MsaG024301 AT1G66600.1 41.860 86 47 1 106 191 80 162 2.02e-15 75.5
MsaG024301 AT4G01250.1 41.860 86 42 2 104 189 106 183 2.45e-15 76.3
MsaG024301 AT4G18170.1 44.681 94 48 2 97 189 136 226 3.69e-15 75.9
MsaG024301 AT1G29860.1 58.621 58 21 1 132 189 136 190 4.50e-15 75.1
MsaG024301 AT5G41570.1 39.231 130 66 7 77 204 51 169 7.95e-15 72.4
MsaG024301 AT4G31550.2 34.783 138 68 5 73 193 172 304 1.12e-14 74.7
MsaG024301 AT1G64000.1 45.333 75 36 2 132 204 114 185 2.14e-14 71.6
MsaG024301 AT5G22570.1 30.570 193 114 4 4 196 2 174 2.28e-14 73.2
MsaG024301 AT5G49520.1 54.237 59 24 1 132 190 221 276 2.76e-14 73.9
MsaG024301 AT1G69310.4 42.857 91 43 3 103 193 123 204 3.54e-14 72.8
MsaG024301 AT1G69310.2 42.857 91 43 3 103 193 123 204 3.54e-14 72.8
MsaG024301 AT1G69310.1 42.857 91 43 3 103 193 123 204 3.54e-14 72.8
MsaG024301 AT1G66560.1 48.333 60 31 0 132 191 103 162 4.01e-14 72.0
MsaG024301 AT5G46350.1 55.172 58 23 1 132 189 183 237 6.68e-14 72.4
MsaG024301 AT1G80590.1 45.455 66 36 0 133 198 86 151 8.98e-14 70.5
MsaG024301 AT1G29280.1 53.333 60 26 1 130 189 72 129 1.22e-13 70.5
MsaG024301 AT4G04450.2 45.349 86 44 2 105 189 170 253 1.30e-13 72.0
MsaG024301 AT5G01900.1 47.458 59 31 0 133 191 111 169 1.53e-13 70.5
MsaG024301 AT2G03340.1 35.897 117 57 3 132 248 415 513 1.55e-13 72.0
MsaG024301 AT2G03340.1 50.000 60 26 2 130 189 248 303 1.67e-11 65.9
MsaG024301 AT2G23320.1 41.176 85 45 3 106 189 215 295 1.77e-13 70.9
MsaG024301 AT4G31550.1 51.613 62 26 2 133 193 247 305 1.92e-13 70.9
MsaG024301 AT4G39410.1 51.515 66 27 2 132 196 223 284 2.35e-13 70.5
MsaG024301 AT4G04450.1 45.349 86 44 2 105 189 264 347 2.99e-13 71.2
MsaG024301 AT2G38470.1 43.333 90 47 2 100 189 152 237 3.08e-13 71.2
MsaG024301 AT4G26440.1 53.125 64 26 2 130 193 176 235 4.44e-13 70.9
MsaG024301 AT4G26440.1 50.847 59 26 1 132 190 372 427 4.31e-11 64.7
MsaG024301 AT2G47260.1 43.820 89 46 2 103 190 144 229 4.82e-13 69.7
MsaG024301 AT2G44745.1 41.758 91 43 3 101 189 117 199 5.96e-13 67.8
MsaG024301 AT5G56270.1 55.000 60 23 2 130 189 271 326 7.17e-13 70.5
MsaG024301 AT3G01080.3 55.000 60 23 2 130 189 203 258 8.26e-13 68.9
MsaG024301 AT2G24570.1 38.835 103 57 4 89 189 200 298 1.08e-12 68.6
MsaG024301 AT5G07100.2 51.724 58 25 1 132 189 141 195 1.33e-12 67.0
MsaG024301 AT5G07100.2 45.763 59 29 1 131 189 23 78 1.99e-11 63.5
MsaG024301 AT5G07100.4 51.724 58 25 1 132 189 141 195 1.33e-12 67.0
MsaG024301 AT5G07100.4 45.763 59 29 1 131 189 23 78 1.99e-11 63.5
MsaG024301 AT5G07100.5 51.724 58 25 1 132 189 141 195 1.33e-12 67.0
MsaG024301 AT5G07100.5 45.763 59 29 1 131 189 23 78 1.99e-11 63.5
MsaG024301 AT5G07100.3 51.724 58 25 1 132 189 141 195 1.33e-12 67.0
MsaG024301 AT5G07100.3 45.763 59 29 1 131 189 23 78 1.99e-11 63.5
MsaG024301 AT5G07100.1 51.724 58 25 1 132 189 234 288 1.36e-12 68.2
MsaG024301 AT5G07100.1 45.763 59 29 1 131 189 116 171 2.02e-11 64.7
MsaG024301 AT4G24240.1 42.353 85 44 3 106 189 256 336 1.37e-12 68.6
MsaG024301 AT1G13960.1 51.724 58 25 1 132 189 409 463 1.41e-12 69.3
MsaG024301 AT1G13960.1 47.619 63 29 2 127 189 224 282 6.00e-12 67.4
MsaG024301 AT2G25000.2 50.000 58 27 1 132 189 103 158 1.50e-12 67.0
MsaG024301 AT2G25000.4 50.000 58 27 1 132 189 103 158 1.50e-12 67.0
MsaG024301 AT1G62300.1 45.679 81 41 2 110 189 289 367 1.57e-12 68.9
MsaG024301 AT3G01080.2 55.000 60 23 2 130 189 203 258 1.89e-12 68.6
MsaG024301 AT3G01080.2 51.724 58 25 1 132 189 344 398 5.28e-12 67.4
MsaG024301 AT2G46130.1 42.667 75 38 2 132 204 30 101 2.10e-12 63.5
MsaG024301 AT2G25000.3 50.000 58 27 1 132 189 125 180 2.22e-12 66.6
MsaG024301 AT3G01080.1 55.000 60 23 2 130 189 165 220 2.27e-12 68.2
MsaG024301 AT3G01080.1 51.724 58 25 1 132 189 306 360 7.18e-12 66.6
MsaG024301 AT3G58710.2 31.176 170 82 6 76 237 22 164 2.42e-12 67.0
MsaG024301 AT1G13960.2 51.724 58 25 1 132 189 256 310 2.95e-12 67.8
MsaG024301 AT1G13960.2 47.619 63 29 2 127 189 71 129 1.12e-11 65.9
MsaG024301 AT2G25000.1 50.000 58 27 1 132 189 146 201 3.10e-12 66.6
MsaG024301 AT2G34830.2 42.857 91 41 3 103 189 196 279 3.12e-12 67.8
MsaG024301 AT2G34830.1 42.857 91 41 3 103 189 187 270 3.16e-12 67.8
MsaG024301 AT5G28650.1 40.476 84 47 2 106 189 237 317 3.36e-12 67.4
MsaG024301 AT4G23550.1 50.877 57 26 1 133 189 141 195 3.42e-12 67.0
MsaG024301 AT2G30250.1 52.632 57 24 1 133 189 329 382 4.89e-12 67.0
MsaG024301 AT4G26640.7 44.000 75 33 2 132 200 341 412 4.99e-12 67.4
MsaG024301 AT4G26640.7 53.448 58 23 2 132 189 171 224 2.58e-11 65.1
MsaG024301 AT4G26640.2 44.000 75 33 2 132 200 381 452 5.70e-12 67.4
MsaG024301 AT4G26640.2 53.448 58 23 2 132 189 211 264 2.79e-11 65.1
MsaG024301 AT4G26640.1 44.000 75 33 2 132 200 309 380 6.10e-12 67.0
MsaG024301 AT4G26640.1 53.448 58 23 2 132 189 139 192 3.00e-11 65.1
MsaG024301 AT5G13080.1 50.847 59 26 1 132 190 67 122 6.89e-12 63.2
MsaG024301 AT3G58710.1 36.111 108 58 3 130 237 69 165 7.70e-12 65.5
MsaG024301 AT2G04880.2 50.877 57 25 1 133 189 284 337 7.74e-12 66.6
MsaG024301 AT2G04880.2 35.714 140 74 5 62 189 29 164 8.87e-11 63.5
MsaG024301 AT4G26640.5 44.000 75 33 2 132 200 205 276 8.15e-12 66.2
MsaG024301 AT4G26640.5 53.448 58 23 2 132 189 35 88 3.30e-11 64.7
MsaG024301 AT4G26640.6 44.000 75 33 2 132 200 205 276 8.15e-12 66.2
MsaG024301 AT4G26640.6 53.448 58 23 2 132 189 35 88 3.30e-11 64.7
MsaG024301 AT4G26640.4 44.000 75 33 2 132 200 205 276 8.15e-12 66.2
MsaG024301 AT4G26640.4 53.448 58 23 2 132 189 35 88 3.30e-11 64.7
MsaG024301 AT4G26640.3 44.000 75 33 2 132 200 205 276 8.15e-12 66.2
MsaG024301 AT4G26640.3 53.448 58 23 2 132 189 35 88 3.30e-11 64.7
MsaG024301 AT2G04880.1 50.877 57 25 1 133 189 308 361 8.70e-12 66.6
MsaG024301 AT2G04880.1 35.714 140 74 5 62 189 29 164 6.63e-11 63.9
MsaG024301 AT1G18860.1 47.368 76 37 2 115 189 221 294 9.51e-12 66.6
MsaG024301 AT4G31800.2 48.276 58 28 1 132 189 111 166 1.08e-11 64.7
MsaG024301 AT5G15130.2 36.667 120 57 5 73 192 143 243 1.41e-11 66.2
MsaG024301 AT2G30590.1 33.945 109 68 3 82 189 263 368 1.47e-11 65.5
MsaG024301 AT4G31800.1 48.276 58 28 1 132 189 176 231 1.50e-11 65.1
MsaG024301 AT5G15130.1 36.667 120 57 5 73 192 185 285 1.53e-11 65.9
MsaG024301 AT3G04670.1 39.286 84 48 2 106 189 237 317 1.55e-11 65.1
MsaG024301 AT5G45050.4 45.714 70 36 1 120 189 1029 1096 1.81e-11 66.2
MsaG024301 AT5G45050.1 45.714 70 36 1 120 189 1168 1235 1.90e-11 66.2
MsaG024301 AT5G43290.1 36.842 114 65 3 116 226 98 207 2.04e-11 64.3
MsaG024301 AT5G45050.2 45.714 70 36 1 120 189 1140 1207 2.06e-11 66.2
MsaG024301 AT1G30650.1 42.857 91 41 3 103 189 189 272 2.28e-11 65.1
MsaG024301 AT1G68150.1 29.897 194 115 7 8 189 106 290 4.71e-11 63.9
MsaG024301 AT4G01720.1 54.386 57 24 1 133 189 240 294 6.19e-11 63.9

Find 96 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGAATAATATTATTATTA+TGG 0.201295 4:+98075761 None:intergenic
TTCCTCTCACCTTGGATGTT+TGG 0.252288 4:+98076069 None:intergenic
GGAGTATTGAAAGGGAAATT+TGG 0.255776 4:+98075349 None:intergenic
ATCAAGTGGGAAACGGAAAT+TGG 0.267866 4:+98075387 None:intergenic
ACCTTCCCTTCCACTTCATA+TGG 0.287302 4:-98075643 MsaT024301.1:CDS
AAATGGAGAATGAATGTAAC+TGG 0.315765 4:-98076629 MsaT024301.1:CDS
TCCCTTGTGCCAAGGGAATA+TGG 0.341878 4:+98076531 None:intergenic
GATGAGAAATAGTTTGATTC+TGG 0.343483 4:+98075520 None:intergenic
TAGTTTGATTCTGGTGTTGC+TGG 0.345432 4:+98075529 None:intergenic
TTGGTAGACGCTGGGGATTC+CGG 0.347235 4:+98076399 None:intergenic
GGCTACAACTGGAGAAAATA+TGG 0.354359 4:-98076112 MsaT024301.1:CDS
TCCGCAACACACAGGACTGC+TGG 0.367448 4:-98075921 MsaT024301.1:CDS
CATCTTCATTTGGTCCTTCA+AGG 0.372550 4:+98076134 None:intergenic
TCTTATCTGATCCATCCATT+TGG 0.372776 4:+98076174 None:intergenic
TCCGGTAACGAAGTTATTAC+TGG 0.373162 4:+98076417 None:intergenic
CTTATCTGATCCATCCATTT+GGG 0.376482 4:+98076175 None:intergenic
CAGTTGTAGCCATCTTCATT+TGG 0.394723 4:+98076124 None:intergenic
TCCAGTAATAACTTCGTTAC+CGG 0.409442 4:-98076418 MsaT024301.1:CDS
TTCAACATGAATGACTTTGA+TGG 0.415973 4:-98075490 MsaT024301.1:CDS
AGGAAAGCATACATGTTCTC+AGG 0.418553 4:-98075839 MsaT024301.1:CDS
AACAACCCTCGTTCAGAATC+CGG 0.427607 4:-98075460 MsaT024301.1:CDS
TAATATTATTATTATGGATG+TGG 0.435488 4:+98075767 None:intergenic
GATACTATCTTCTTATGAAA+AGG 0.436019 4:-98076493 MsaT024301.1:CDS
AATATTATTATTATGGATGT+GGG 0.437122 4:+98075768 None:intergenic
TCGTTCAGAATCCGGCATTA+CGG 0.438155 4:-98075452 MsaT024301.1:CDS
AAATTTGGATCTATTTCCAC+TGG 0.446466 4:+98075364 None:intergenic
CCTTCCCTTCCACTTCATAT+GGG 0.447528 4:-98075642 MsaT024301.1:CDS
CTGGAGATAATAAGTGTGTC+TGG 0.447945 4:+98075548 None:intergenic
AGAATTTGTTACTGATGTAT+TGG 0.449729 4:+98075417 None:intergenic
CCTCTGAGCGAGGACTTTGA+TGG 0.453832 4:-98076369 MsaT024301.1:CDS
ACCTTGGATGTTTGGTGCCA+AGG 0.456940 4:+98076077 None:intergenic
TTGTGACTTATATGGCTCAA+TGG 0.457594 4:+98075807 None:intergenic
AACTTCTTGGTGATCTTGGA+AGG 0.459080 4:+98076344 None:intergenic
TCAGAAAGGTTGCGCCCAAA+TGG 0.459805 4:-98076189 MsaT024301.1:intron
TCAAGTGGGAAACGGAAATT+GGG 0.461042 4:+98075388 None:intergenic
ACAATTGCAGAAGTTACTAT+AGG 0.468017 4:-98075951 MsaT024301.1:intron
AGCTGTGACAGTAGCCTTGA+AGG 0.468608 4:-98076148 MsaT024301.1:CDS
GACTCCCATTGGTAGACGCT+GGG 0.469167 4:+98076391 None:intergenic
CTCATCAATGAGTTAATTCA+AGG 0.471281 4:-98076594 MsaT024301.1:CDS
CAAATGAAGATGGCTACAAC+TGG 0.475643 4:-98076123 MsaT024301.1:CDS
TCAACATGAATGACTTTGAT+GGG 0.485787 4:-98075489 MsaT024301.1:CDS
AATTTCCCTTTCAATACTCC+AGG 0.487060 4:-98075346 MsaT024301.1:CDS
GGGAAACGGAAATTGGGAAT+AGG 0.487728 4:+98075394 None:intergenic
ATATTTGACATAACTTATAG+AGG 0.489229 4:-98075859 MsaT024301.1:CDS
ATGTCATTTCTTCGTTTCCC+AGG 0.491525 4:+98075674 None:intergenic
CAACGCAGGAAAACCACAGC+TGG 0.492451 4:-98075615 MsaT024301.1:CDS
GACTGTGAAAACAGATAACC+TGG 0.505158 4:-98075692 MsaT024301.1:CDS
AAGTCATTCATGTTGAAAGA+TGG 0.505789 4:+98075496 None:intergenic
CTCTGAGCGAGGACTTTGAT+GGG 0.512988 4:-98076368 MsaT024301.1:CDS
ATTTGTTACTGATGTATTGG+TGG 0.517341 4:+98075420 None:intergenic
GGAAATGATCTCCGTAATGC+CGG 0.517483 4:+98075441 None:intergenic
CCTTGGCACAAGGGATTCAC+AGG 0.521370 4:-98076523 MsaT024301.1:CDS
CCGCAACACACAGGACTGCT+GGG 0.524602 4:-98075920 MsaT024301.1:CDS
AAAGGTTGCGCCCAAATGGA+TGG 0.526632 4:-98076185 MsaT024301.1:CDS
CCACTGGATCAAGTGGGAAA+CGG 0.528076 4:+98075380 None:intergenic
CTGTGAATCCCTTGTGCCAA+GGG 0.529447 4:+98076524 None:intergenic
CACCATATTCCCTTGGCACA+AGG 0.530203 4:-98076533 MsaT024301.1:CDS
GAATCCCCAGCGTCTACCAA+TGG 0.531265 4:-98076396 MsaT024301.1:CDS
CCCAGCAGTCCTGTGTGTTG+CGG 0.534772 4:+98075920 None:intergenic
CCGTTTCCCACTTGATCCAG+TGG 0.535887 4:-98075380 MsaT024301.1:CDS
TAAGTTATGTCAAATATTGT+CGG 0.537763 4:+98075865 None:intergenic
AATCCCCAGCGTCTACCAAT+GGG 0.538110 4:-98076395 MsaT024301.1:CDS
TTTCTTGTGATTGTTGTGCA+TGG 0.548111 4:+98075740 None:intergenic
GGACTCCCATTGGTAGACGC+TGG 0.553945 4:+98076390 None:intergenic
GAAGTGGAAGGGAAGGTGAA+AGG 0.559123 4:+98075649 None:intergenic
TTGAGGACACCATATTCCCT+TGG 0.560526 4:-98076540 MsaT024301.1:CDS
AAAAGCCTGGAGTATTGAAA+GGG 0.560663 4:+98075341 None:intergenic
GGAAAGCATACATGTTCTCA+GGG 0.560758 4:-98075838 MsaT024301.1:CDS
CTGGAAAAGACTGCTCAAGA+AGG 0.564100 4:+98075567 None:intergenic
CTTGAAGGACCAAATGAAGA+TGG 0.570659 4:-98076133 MsaT024301.1:CDS
CCCATATGAAGTGGAAGGGA+AGG 0.572985 4:+98075642 None:intergenic
TCTATTTCCACTGGATCAAG+TGG 0.573162 4:+98075373 None:intergenic
CAATGAGTTAATTCAAGGGA+TGG 0.575038 4:-98076589 MsaT024301.1:CDS
CTCGCTCAGAGGACTCCCAT+TGG 0.583201 4:+98076380 None:intergenic
GTGTACCCATATGAAGTGGA+AGG 0.589053 4:+98075637 None:intergenic
GCAGTCCTGTGTGTTGCGGA+AGG 0.591799 4:+98075924 None:intergenic
ACCATATTCCCTTGGCACAA+GGG 0.601275 4:-98076532 MsaT024301.1:CDS
AGGCTGGCGGAACCCAGCTG+TGG 0.617131 4:+98075602 None:intergenic
ATATTATTATTATGGATGTG+GGG 0.623210 4:+98075769 None:intergenic
TTCATATGGGTACACAACGC+AGG 0.623458 4:-98075629 MsaT024301.1:CDS
CCATCAAAGTCCTCGCTCAG+AGG 0.628233 4:+98076369 None:intergenic
CCTGTGAATCCCTTGTGCCA+AGG 0.629078 4:+98076523 None:intergenic
CGTTGTGTACCCATATGAAG+TGG 0.630275 4:+98075633 None:intergenic
TCCTTGGCACCAAACATCCA+AGG 0.635439 4:-98076078 MsaT024301.1:intron
AACGCAGGAAAACCACAGCT+GGG 0.639928 4:-98075614 MsaT024301.1:CDS
CAATGGGAGTCCTCTGAGCG+AGG 0.641184 4:-98076379 MsaT024301.1:CDS
GTAATGCCGGATTCTGAACG+AGG 0.646895 4:+98075454 None:intergenic
AGAGATTGAAAGAAGACTTG+AGG 0.648506 4:-98076557 MsaT024301.1:CDS
TGTACCCATATGAAGTGGAA+GGG 0.653842 4:+98075638 None:intergenic
AATGGAGAATGAATGTAACT+GGG 0.656029 4:-98076628 MsaT024301.1:CDS
TCATCAATGAGTTAATTCAA+GGG 0.662966 4:-98076593 MsaT024301.1:CDS
CTATTTCCACTGGATCAAGT+GGG 0.677998 4:+98075374 None:intergenic
GTGCACCTTCCGCAACACAC+AGG 0.679677 4:-98075929 MsaT024301.1:CDS
ACTCCCATTGGTAGACGCTG+GGG 0.692667 4:+98076392 None:intergenic
TAATGCCGGATTCTGAACGA+GGG 0.706809 4:+98075455 None:intergenic
ACTGTGAAAACAGATAACCT+GGG 0.720036 4:-98075691 MsaT024301.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 98075333 98076649 98075333 ID=MsaG024301
Chr4 mRNA 98075333 98076649 98075333 ID=MsaT024301.1;Parent=MsaG024301
Chr4 exon 98075333 98075963 98075333 ID=MsaT024301.1.exon3;Parent=MsaT024301.1
Chr4 CDS 98075333 98075963 98075333 ID=cds.MsaT024301.1;Parent=MsaT024301.1
Chr4 exon 98076079 98076207 98076079 ID=MsaT024301.1.exon2;Parent=MsaT024301.1
Chr4 CDS 98076079 98076207 98076079 ID=cds.MsaT024301.1;Parent=MsaT024301.1
Chr4 exon 98076324 98076649 98076324 ID=MsaT024301.1.exon1;Parent=MsaT024301.1
Chr4 CDS 98076324 98076649 98076324 ID=cds.MsaT024301.1;Parent=MsaT024301.1
Gene Sequence

>MsaT024301.1

ATGGAGAATGAATGTAACTGGGAGCAAAACACACTCATCAATGAGTTAATTCAAGGGATGGATGTAGCAAAGAGATTGAAAGAAGACTTGAGGACACCATATTCCCTTGGCACAAGGGATTCACAGGTGCAGATGATACTATCTTCTTATGAAAAGGCTTTACAAATTTTAAGATGTAATGAATCAACTTCTAAGTCACAGACAGTGACTCCAGTAATAACTTCGTTACCGGAATCCCCAGCGTCTACCAATGGGAGTCCTCTGAGCGAGGACTTTGATGGGACCTTCCAAGATCACCAAGAAGTTAAAAACAAATCAAAGAAAAGAAAGGTTGCGCCCAAATGGATGGATCAGATAAGAGTGAGCTGTGACAGTAGCCTTGAAGGACCAAATGAAGATGGCTACAACTGGAGAAAATATGGTCAAAAAGATATCCTTGGCACCAAACATCCAAGAAGTTACTATAGGTGCACCTTCCGCAACACACAGGACTGCTGGGCGACGAAGCAAGTGCAGAGATCAGATGAAGATCCGACAATATTTGACATAACTTATAGAGGAAAGCATACATGTTCTCAGGGAAACAATGCCATTGAGCCATATAAGTCACAAGAAAAACAAGAGAAACCCCACATCCATAATAATAATATTATTCACCATGCACAACAATCACAAGAAAGCTTTACAAATTTCAGCAACACCTTGACTGTGAAAACAGATAACCTGGGAAACGAAGAAATGACATGTCCTTTCACCTTCCCTTCCACTTCATATGGGTACACAACGCAGGAAAACCACAGCTGGGTTCCGCCAGCCTTAGAAAACGATTCCTTCTTGAGCAGTCTTTTCCAGACACACTTATTATCTCCAGCAACACCAGAATCAAACTATTTCTCATCTCCATCTTTCAACATGAATGACTTTGATGGGTTCTATAACAACCCTCGTTCAGAATCCGGCATTACGGAGATCATTTCCACCAATACATCAGTAACAAATTCTCCTATTCCCAATTTCCGTTTCCCACTTGATCCAGTGGAAATAGATCCAAATTTCCCTTTCAATACTCCAGGCTTTTTCTCCTAA
Protein sequence

>MsaT024301.1

MENECNWEQNTLINELIQGMDVAKRLKEDLRTPYSLGTRDSQVQMILSSYEKALQILRCNESTSKSQTVTPVITSLPESPASTNGSPLSEDFDGTFQDHQEVKNKSKKRKVAPKWMDQIRVSCDSSLEGPNEDGYNWRKYGQKDILGTKHPRSYYRCTFRNTQDCWATKQVQRSDEDPTIFDITYRGKHTCSQGNNAIEPYKSQEKQEKPHIHNNNIIHHAQQSQESFTNFSNTLTVKTDNLGNEEMTCPFTFPSTSYGYTTQENHSWVPPALENDSFLSSLFQTHLLSPATPESNYFSSPSFNMNDFDGFYNNPRSESGITEIISTNTSVTNSPIPNFRFPLDPVEIDPNFPFNTPGFFS*