AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG024337

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024337 MtrunA17_Chr8g0388341 95.149 1278 49 4 1 1273 1 1270 0.0 2437
MsaG024337 MtrunA17_Chr4g0061801 33.514 185 89 10 35 210 34 193 8.33e-17 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024337 AT4G10930.1 49.212 571 249 13 476 1033 414 956 5.60e-146 473
MsaG024337 AT4G10930.1 46.789 327 129 7 1 300 1 309 1.74e-77 280
MsaG024337 AT4G10930.2 49.212 571 249 13 476 1033 409 951 5.74e-146 473
MsaG024337 AT4G10930.2 46.789 327 129 7 1 300 1 309 2.16e-77 280
MsaG024337 AT3G05670.3 30.811 185 104 7 7 184 382 549 2.08e-15 82.0
MsaG024337 AT3G05670.1 30.811 185 104 7 7 184 382 549 2.08e-15 82.0
MsaG024337 AT3G05670.2 30.811 185 104 7 7 184 382 549 2.08e-15 82.0

Find 272 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGGAGCTCGCTCTGATTC+TGG 0.072097 4:-98407820 None:intergenic
TAATTTCCTTCCTTAGATTT+TGG 0.133665 4:-98407453 None:intergenic
AGGCCTCTCTCACTTGTTTC+TGG 0.153394 4:-98404992 None:intergenic
CATTCACTGTTAGTATTATC+TGG 0.160173 4:-98404884 None:intergenic
TTTATAAGTGGTTCTTTATC+AGG 0.190528 4:-98410083 None:intergenic
CTTTCACAGAATCAGATTTA+AGG 0.198788 4:-98408167 None:intergenic
CTTCGTGCATAGCGATTTAA+AGG 0.212127 4:+98405171 MsaT024337.1:CDS
AAAATCTTCTCGAAGCCTTT+AGG 0.231661 4:+98407530 MsaT024337.1:CDS
TGAAAGGTGATAAAAGAAAA+TGG 0.240611 4:+98408184 MsaT024337.1:CDS
TAATTCGTAACAGGATAATT+TGG 0.241541 4:+98401222 MsaT024337.1:intron
GGCCAGCCTTCCGTTTCTTC+TGG 0.249709 4:+98409999 MsaT024337.1:CDS
GATATAATGAATATAGTTAA+AGG 0.267083 4:+98407289 MsaT024337.1:CDS
CCTTCCAAAGGAAAGTCTTC+TGG 0.270839 4:-98405519 None:intergenic
TCATCGGTCCCGGATGTATT+AGG 0.271710 4:-98410287 None:intergenic
GCTTTGTATGTATTGACAAC+TGG 0.277151 4:+98401556 MsaT024337.1:CDS
ATGCAAAATTGAATCTGATA+TGG 0.280001 4:+98405276 MsaT024337.1:CDS
GGGTCTTCATCTTGGTTTAT+CGG 0.282538 4:+98405297 MsaT024337.1:CDS
CTGGCCAATCTCTGCATTAA+TGG 0.289046 4:-98409982 None:intergenic
AGTCGCGTTCTTCTTCCTTA+TGG 0.300027 4:-98410181 None:intergenic
GCAAGTGTTGAAGAAGATTC+TGG 0.303824 4:+98403135 MsaT024337.1:CDS
AAATCTTCTCGAAGCCTTTA+GGG 0.304999 4:+98407531 MsaT024337.1:CDS
GTCTCATATCAGTTGGCAAT+TGG 0.314592 4:-98408414 None:intergenic
GCCTTTAGGGCTGCTATAAC+AGG 0.315732 4:+98407544 MsaT024337.1:CDS
AACAGATGGCAATTCAATAA+AGG 0.316631 4:+98404841 MsaT024337.1:CDS
TCAGAATTTGAAGATTTATA+CGG 0.316787 4:+98410059 MsaT024337.1:CDS
TCTGGAAAGAATTGGATTCT+TGG 0.322471 4:-98407851 None:intergenic
CGGGAGCTCGCTCTGATTCT+GGG 0.327606 4:-98407819 None:intergenic
GGGATTGTGAAATTGAGTTT+TGG 0.333583 4:+98407710 MsaT024337.1:CDS
CATTAATGGTGGAATCCTTC+TGG 0.335776 4:-98409968 None:intergenic
CCTACATCAAGGAGCATATC+AGG 0.338678 4:+98410719 MsaT024337.1:CDS
CCTCACTAGTCCAAAATCTA+AGG 0.343630 4:+98407443 MsaT024337.1:CDS
TGGATTCTTGGCCTGACATT+CGG 0.351968 4:-98407839 None:intergenic
TGATCAACTGAAACTCATTC+TGG 0.363424 4:-98401608 None:intergenic
CTTGCGATTCATGTGATCTA+TGG 0.364336 4:+98403175 MsaT024337.1:CDS
TGAAGCTTCAACCGATAGTT+CGG 0.367125 4:-98407137 None:intergenic
GACTTAGACAAAGCAGTAAA+TGG 0.369446 4:+98410200 MsaT024337.1:CDS
TATTATCTGGGTTAAATTCC+AGG 0.369880 4:-98404871 None:intergenic
GATGCTGCAATCTCCTTTGT+TGG 0.370503 4:-98407265 None:intergenic
ATATACAATCTGGAAAGAAT+TGG 0.372942 4:-98407859 None:intergenic
GCATCAGTTTCTTCTTCAGC+TGG 0.373052 4:+98408117 MsaT024337.1:CDS
GAATGCTAATTTCCTCATAA+AGG 0.377921 4:+98410837 MsaT024337.1:CDS
CGTGAACCGAGTAGCTTTGA+TGG 0.379852 4:+98405214 MsaT024337.1:CDS
GGATTCTTGGCCTGACATTC+GGG 0.380821 4:-98407838 None:intergenic
TTTCTGGAATCCATTTGTTT+CGG 0.382216 4:-98404976 None:intergenic
CTTTCTCACCTTCCTTTATG+AGG 0.383794 4:-98410849 None:intergenic
GGTTATTGACAGGGGAGTAT+TGG 0.388180 4:+98401282 MsaT024337.1:CDS
TCTGATATGGGTCTTCATCT+TGG 0.392960 4:+98405289 MsaT024337.1:CDS
TTTCTCAGATAAACAAACTT+TGG 0.395771 4:-98408031 None:intergenic
TCTTCAAATTCTGAATCAAC+TGG 0.396421 4:-98410050 None:intergenic
TACATATTTCATTGTTCTTG+TGG 0.397541 4:-98409650 None:intergenic
CATTTGACTAGCAGCTGCTC+TGG 0.397549 4:-98407170 None:intergenic
TCCACCATTAATGCAGAGAT+TGG 0.398293 4:+98409978 MsaT024337.1:CDS
TGCACGAAGGATTTAGAAGT+TGG 0.400122 4:-98405157 None:intergenic
TTGTCATCAACAGATTGATT+TGG 0.401400 4:-98407967 None:intergenic
CAAATCCTCTATAAACTTTG+AGG 0.403161 4:+98407492 MsaT024337.1:CDS
AGATTCCTTTGTGCCAACTT+TGG 0.404039 4:-98408142 None:intergenic
ACAGATGGCAATTCAATAAA+GGG 0.406400 4:+98404842 MsaT024337.1:CDS
TTCAACTTTATTGCTCCCTA+TGG 0.408089 4:-98401880 None:intergenic
TTTGTAGGCAGTTACTTGCT+TGG 0.409234 4:+98403080 MsaT024337.1:intron
GAAGAAAACAAATAATGCTT+TGG 0.415132 4:+98409863 MsaT024337.1:CDS
AAAGTTGGCACAAAGGAATC+TGG 0.416752 4:+98408144 MsaT024337.1:CDS
CTTACCTGCCTCACGGATAC+AGG 0.418225 4:-98408469 None:intergenic
TTCAACGCTATTTGAACTGC+AGG 0.418515 4:-98409588 None:intergenic
AATGATTGTAATTCACCATC+TGG 0.419965 4:-98410113 None:intergenic
ATGTGGAATATGTATGGATA+TGG 0.420646 4:+98401261 MsaT024337.1:CDS
AGTTGTGTCTGTGTTTATGT+TGG 0.421385 4:-98409496 None:intergenic
GAAGCTTCAACCGATAGTTC+GGG 0.423173 4:-98407136 None:intergenic
ACATATTTCATTGTTCTTGT+GGG 0.424032 4:-98409649 None:intergenic
TGTTTAGTCTGTTCCGAGAA+AGG 0.424635 4:-98407922 None:intergenic
GATTCCAGAAGACTTTCCTT+TGG 0.426336 4:+98405515 MsaT024337.1:CDS
TTGTTCTTGTGGGGACTGCT+TGG 0.427164 4:-98409639 None:intergenic
CATCAACTTCAGGAGGCAAT+AGG 0.428681 4:-98405012 None:intergenic
TGTATCTGCTATATACAATC+TGG 0.430477 4:-98407869 None:intergenic
TGCACGCTTTCTTCTACCAT+TGG 0.430540 4:-98407680 None:intergenic
TCTTCAGCTGGTGCCAAAGT+TGG 0.431339 4:+98408129 MsaT024337.1:CDS
TGTAATGAAAAGGAAGTGTC+AGG 0.431539 4:+98409669 MsaT024337.1:CDS
ACTGCATCTCTGTGACAGTT+TGG 0.432384 4:-98409945 None:intergenic
GTCTGTGTCCATTGCTGATA+CGG 0.433476 4:+98404922 MsaT024337.1:CDS
GGAAACATGTGGAATATGTA+TGG 0.436547 4:+98401255 MsaT024337.1:CDS
CAGGATAATTTGGACGTTGA+AGG 0.438552 4:+98401232 MsaT024337.1:intron
CCCTGTTATAGCAGCCCTAA+AGG 0.443432 4:-98407545 None:intergenic
TTTGGATAGTGCAGACTGTA+AGG 0.443762 4:+98409881 MsaT024337.1:CDS
TCTCATATCAGTTGGCAATT+GGG 0.444054 4:-98408413 None:intergenic
TATTGCCTCCTGAAGTTGAT+GGG 0.446085 4:+98405014 MsaT024337.1:CDS
GAAGAAAGCGTGCATGGGAT+AGG 0.446500 4:+98407689 MsaT024337.1:CDS
TGCAAAATTGAATCTGATAT+GGG 0.448382 4:+98405277 MsaT024337.1:CDS
CTATTGCCTCCTGAAGTTGA+TGG 0.451632 4:+98405013 MsaT024337.1:CDS
ATGAAGAGAGTTTCTGATGA+TGG 0.452594 4:+98407412 MsaT024337.1:CDS
TTGAAGATGGCTCATTCCTC+AGG 0.453779 4:+98401898 MsaT024337.1:CDS
AGCAAACCTCCTAATACATC+CGG 0.453834 4:+98410278 MsaT024337.1:CDS
TTCTTTGCCTTCAGAGCTGA+AGG 0.455017 4:-98407598 None:intergenic
TTCTTGTGGGGACTGCTTGG+AGG 0.456656 4:-98409636 None:intergenic
CAAAATAATCCGTCAGCAAA+AGG 0.457161 4:+98407943 MsaT024337.1:CDS
CAATGGTTGACCGAAACAAA+TGG 0.457635 4:+98404966 MsaT024337.1:CDS
GTATGGATATGGTTATTGAC+AGG 0.458801 4:+98401272 MsaT024337.1:CDS
TCATTGTTTGACTTCTGGGC+AGG 0.459693 4:-98410344 None:intergenic
AGGATAATTTGGACGTTGAA+GGG 0.461659 4:+98401233 MsaT024337.1:intron
GCAGTAAATGGTGCTGAAGT+AGG 0.462919 4:+98410212 MsaT024337.1:CDS
GATTTAAAGGAAAGTGATAA+TGG 0.463962 4:+98405184 MsaT024337.1:CDS
GTGTCCGACATTAACTTACA+TGG 0.464150 4:-98405052 None:intergenic
TTTAAAACATTCTCCAACAA+AGG 0.466040 4:+98407252 MsaT024337.1:CDS
AGAGGATGTCAAACCTTTCT+CGG 0.466789 4:+98407909 MsaT024337.1:CDS
AGAGCGAGCTCCCGAATGTC+AGG 0.468530 4:+98407828 MsaT024337.1:CDS
GAACACAGCAAATGAAAGTC+AGG 0.475761 4:+98408248 MsaT024337.1:CDS
CTTCATCTTGGTTTATCGGT+GGG 0.477646 4:+98405301 MsaT024337.1:CDS
GAAAAGAAAGAAAACATGAC+TGG 0.479402 4:+98407370 MsaT024337.1:CDS
GAGAGCTTCAAAACCTGAAA+AGG 0.481141 4:+98407747 MsaT024337.1:CDS
TGAAACTCATTCTGGCAAAG+TGG 0.481558 4:-98401600 None:intergenic
ATATAATGAATATAGTTAAA+GGG 0.482474 4:+98407290 MsaT024337.1:CDS
CATTGTTTGACTTCTGGGCA+GGG 0.482806 4:-98410343 None:intergenic
AACAGATCCCCATCAACTTC+AGG 0.482991 4:-98405022 None:intergenic
CAATGAAATATGTAATGAAA+AGG 0.483896 4:+98409659 MsaT024337.1:CDS
GACAGCTGTGGTTGTCTCAA+TGG 0.485598 4:+98404949 MsaT024337.1:CDS
ATTCACTGTTAGTATTATCT+GGG 0.485781 4:-98404883 None:intergenic
CCTCTTTCTACCAGCCACTC+TGG 0.486616 4:-98405729 None:intergenic
CCAGAAGACTTTCCTTTGGA+AGG 0.487768 4:+98405519 MsaT024337.1:CDS
GGTGAGCAAGAGGCACCTCC+TGG 0.488201 4:-98409924 None:intergenic
TGATAAAAGAAAATGGGCCT+TGG 0.488612 4:+98408191 MsaT024337.1:CDS
GAAAGGTGATAAAAGAAAAT+GGG 0.488760 4:+98408185 MsaT024337.1:CDS
ATGATGACTGGTCGATTGAC+GGG 0.490445 4:+98402314 MsaT024337.1:CDS
GTCCAGAAGAAACGGAAGGC+TGG 0.493839 4:-98410001 None:intergenic
TGAAAATCAAGTAGTGAAGA+AGG 0.495591 4:+98410364 MsaT024337.1:CDS
TTGTCGTTGGCATCATCAAA+TGG 0.495748 4:-98401068 None:intergenic
GTTACTTGCTTGGATGGTGA+TGG 0.499617 4:+98403090 MsaT024337.1:CDS
AATTCCTGTATCCGTGAGGC+AGG 0.501209 4:+98408465 MsaT024337.1:CDS
TCTAGTTCAAGTATATCATC+AGG 0.502187 4:-98409693 None:intergenic
CGAGAATAATGATTGCCATA+AGG 0.502487 4:+98410166 MsaT024337.1:CDS
GATGGCTGCAAAATTCGAAA+TGG 0.503330 4:+98403108 MsaT024337.1:CDS
AGATGCTATTAATATTGAAA+AGG 0.504588 4:+98409389 MsaT024337.1:CDS
CAGTAAATGGTGCTGAAGTA+GGG 0.505019 4:+98410213 MsaT024337.1:CDS
TAAAAGAAAATGGGCCTTGG+AGG 0.505512 4:+98408194 MsaT024337.1:CDS
TTATAAGTGGTTCTTTATCA+GGG 0.505721 4:-98410082 None:intergenic
TCTTCAATAAGCGTTCCGTC+AGG 0.505803 4:-98409314 None:intergenic
CTTAATTGTACTTTACCAGC+TGG 0.506554 4:-98408078 None:intergenic
TAGGCAGTTACTTGCTTGGA+TGG 0.508085 4:+98403084 MsaT024337.1:intron
GATGATGACTGGTCGATTGA+CGG 0.511297 4:+98402313 MsaT024337.1:CDS
CTTTGTATGTATTGACAACT+GGG 0.512954 4:+98401557 MsaT024337.1:CDS
AGTATCAAATCCCACACAAA+AGG 0.513757 4:-98403732 None:intergenic
GTGAAGGTATATGATACCAT+AGG 0.518587 4:+98401864 MsaT024337.1:intron
TCTTCATCTTGGTTTATCGG+TGG 0.519376 4:+98405300 MsaT024337.1:CDS
TATTGGATTGCTGTCAGCAC+TGG 0.521780 4:+98401299 MsaT024337.1:CDS
TTCTGATTGATCAGTTTGCA+AGG 0.523545 4:-98409538 None:intergenic
AAGATTGTACGTGGTCCAGC+TGG 0.523778 4:+98408063 MsaT024337.1:CDS
TTCAACCGATAGTTCGGGGT+TGG 0.525228 4:-98407131 None:intergenic
GCACGAAGGATTTAGAAGTT+GGG 0.526215 4:-98405156 None:intergenic
AATGGACACAGACACTATTC+CGG 0.526465 4:-98404912 None:intergenic
CAAGTCCCTTGGAAAGCTTA+CGG 0.528945 4:-98407318 None:intergenic
GTTATCCCCTTCAGCTCTGA+AGG 0.529362 4:+98407591 MsaT024337.1:CDS
ATTGGACTCATCAAATAACT+TGG 0.529746 4:-98405239 None:intergenic
AAGAAAGCGTGCATGGGATA+GGG 0.529759 4:+98407690 MsaT024337.1:CDS
GGAGGTTTGCTAGTGATGGT+AGG 0.529923 4:-98410266 None:intergenic
AAGAAAACATGACTGGGTTG+AGG 0.531917 4:+98407377 MsaT024337.1:CDS
TGGTAGGAGTCCAGAAGAAA+CGG 0.532812 4:-98410009 None:intergenic
GATGACAGTGCCAGAGTGGC+TGG 0.534895 4:+98405719 MsaT024337.1:CDS
GTTAGGGATCTTTGTAACAC+TGG 0.536237 4:-98409784 None:intergenic
AGTCTTCTGGAATCGAGCAC+GGG 0.536505 4:-98405506 None:intergenic
CCTGATATGCTCCTTGATGT+AGG 0.537367 4:-98410719 None:intergenic
AGTTCTTCTCCCTTCATGAT+CGG 0.538187 4:-98405115 None:intergenic
GAGGTTTGCTAGTGATGGTA+GGG 0.539545 4:-98410265 None:intergenic
CATATTTCATTGTTCTTGTG+GGG 0.541455 4:-98409648 None:intergenic
CCAGAGTGGCTGGTAGAAAG+AGG 0.542880 4:+98405729 MsaT024337.1:CDS
CTTAAATCTGATTCTGTGAA+AGG 0.542950 4:+98408168 MsaT024337.1:CDS
ATTAGGAGGTTTGCTAGTGA+TGG 0.546641 4:-98410270 None:intergenic
AGAGGAAGTTACATTGTCGT+TGG 0.546781 4:-98401081 None:intergenic
ATGGTAGAAGAAAGCGTGCA+TGG 0.547779 4:+98407683 MsaT024337.1:CDS
AGAAAACATGACTGGGTTGA+GGG 0.548051 4:+98407378 MsaT024337.1:CDS
GAAACTCATTCTGGCAAAGT+GGG 0.548840 4:-98401599 None:intergenic
ATTATCTGGGTTAAATTCCA+GGG 0.551587 4:-98404870 None:intergenic
TGATCACGGCTGAACAGTAC+AGG 0.553038 4:+98410761 MsaT024337.1:CDS
TGCTATTAATATTGAAAAGG+AGG 0.553296 4:+98409392 MsaT024337.1:CDS
GGGAGCAATAAAGTTGAAGA+TGG 0.554268 4:+98401885 MsaT024337.1:CDS
TCTGCAGTTCTACGAATGAT+TGG 0.555363 4:-98409348 None:intergenic
GCTAATTTCCTCATAAAGGA+AGG 0.555452 4:+98410841 MsaT024337.1:CDS
ACTAGAATCTCATCCCGAGC+TGG 0.555715 4:+98409710 MsaT024337.1:CDS
CTTTGCCTTCAGAGCTGAAG+GGG 0.557451 4:-98407596 None:intergenic
TCTGAAAACAATGAAGTGCC+AGG 0.557715 4:+98409906 MsaT024337.1:CDS
CGATGAAAATATTCGAAGGA+AGG 0.557810 4:+98410304 MsaT024337.1:CDS
AGATGCCAACCCCGAACTAT+CGG 0.558854 4:+98407126 MsaT024337.1:CDS
TCTGATTGATCAGTTTGCAA+GGG 0.559641 4:-98409537 None:intergenic
GGATAATTTGGACGTTGAAG+GGG 0.562397 4:+98401234 MsaT024337.1:intron
GGACACAAATAATCAGCTGC+AGG 0.564443 4:+98405069 MsaT024337.1:CDS
GATAAATTACGTACAAGCAA+AGG 0.571388 4:-98408300 None:intergenic
TTGGATAGTGCAGACTGTAA+GGG 0.571843 4:+98409882 MsaT024337.1:CDS
TCAATAAGCGTTCCGTCAGG+CGG 0.572262 4:-98409311 None:intergenic
AACTGTGTCACCGATCATGA+AGG 0.573404 4:+98405105 MsaT024337.1:CDS
AAGTCTTCTGGAATCGAGCA+CGG 0.575721 4:-98405507 None:intergenic
TGCAAGGGTGATGATGAGGT+TGG 0.580649 4:-98409522 None:intergenic
TCTGTGTCCATTGCTGATAC+GGG 0.582687 4:+98404923 MsaT024337.1:CDS
ATCAGGCCATTGTGCAAGAG+TGG 0.583842 4:+98410736 MsaT024337.1:CDS
TGAAGGTATATGATACCATA+GGG 0.583986 4:+98401865 MsaT024337.1:intron
GGTTTGACATCCTCTTTCCG+TGG 0.585428 4:-98407901 None:intergenic
ACTAGTCCAAAATCTAAGGA+AGG 0.586333 4:+98407447 MsaT024337.1:CDS
TTGGAAGATGATGATTACAT+AGG 0.587979 4:+98409759 MsaT024337.1:CDS
TCGGTCCCGGATGTATTAGG+AGG 0.590138 4:-98410284 None:intergenic
TCTGGACTCCTACCATGTGA+AGG 0.590540 4:+98410017 MsaT024337.1:CDS
GCAGTTTCTAAAGAAACAGA+TGG 0.591651 4:+98404827 MsaT024337.1:CDS
GTTTGACATCCTCTTTCCGT+GGG 0.592195 4:-98407900 None:intergenic
TCACAGAGATGCAGTCCAGA+AGG 0.593323 4:+98409953 MsaT024337.1:CDS
TGATATGCTGACACTCTCAC+AGG 0.594250 4:+98401043 MsaT024337.1:CDS
TATGGATATGGTTATTGACA+GGG 0.594947 4:+98401273 MsaT024337.1:CDS
AACACCGGTCTCATATCAGT+TGG 0.595062 4:-98408421 None:intergenic
GCAAGTTCGTATAAAAGTTG+AGG 0.595408 4:+98407099 MsaT024337.1:CDS
GTGACAGTTTGGTGAGCAAG+AGG 0.595565 4:-98409934 None:intergenic
GCCAATCTCTGCATTAATGG+TGG 0.596293 4:-98409979 None:intergenic
ATTAGTGTCAGCAAGTCCCT+TGG 0.597733 4:-98407329 None:intergenic
ATTGCCTCCTGAAGTTGATG+GGG 0.598756 4:+98405015 MsaT024337.1:CDS
GACGTTGAAGGGGAAACATG+TGG 0.600689 4:+98401244 MsaT024337.1:CDS
GACGAACCGTAAGCTTTCCA+AGG 0.600753 4:+98407312 MsaT024337.1:CDS
TGTCTCTCCCGTATCAGCAA+TGG 0.601856 4:-98404930 None:intergenic
AAGAAGTCAATGTTGCAGAA+AGG 0.602008 4:+98407616 MsaT024337.1:CDS
ACAGTACGTGGTTATGCCCA+AGG 0.604432 4:+98403768 MsaT024337.1:CDS
ACTAACAGTGAATGTCATGC+CGG 0.606011 4:+98404893 MsaT024337.1:CDS
TGGATAGTGCAGACTGTAAG+GGG 0.606342 4:+98409883 MsaT024337.1:CDS
AATTAAGCAGTCACTCAGGA+GGG 0.606780 4:+98408094 MsaT024337.1:CDS
AACTGATATGAGACCGGTGT+TGG 0.607099 4:+98408423 MsaT024337.1:CDS
ATTACAATCATTACATGGAG+AGG 0.607432 4:+98410124 MsaT024337.1:CDS
CAACAGCACCTTCACATGGT+AGG 0.607744 4:-98410025 None:intergenic
TTACAATCATTACATGGAGA+GGG 0.608719 4:+98410125 MsaT024337.1:CDS
TAGAACTGCAGACACAGAGT+TGG 0.609167 4:+98409359 MsaT024337.1:CDS
TGGCGACAAGGTGCCAACAC+CGG 0.609390 4:-98408436 None:intergenic
TGTACTTCTAGTCGATAATG+TGG 0.609798 4:+98405449 MsaT024337.1:intron
TGGTAGAAGAAAGCGTGCAT+GGG 0.610649 4:+98407684 MsaT024337.1:CDS
TCTTTGCCTTCAGAGCTGAA+GGG 0.611101 4:-98407597 None:intergenic
CAATTAAGCAGTCACTCAGG+AGG 0.619637 4:+98408093 MsaT024337.1:CDS
TTGGTTCCATCAAAGCTACT+CGG 0.620668 4:-98405220 None:intergenic
CTTTAAATCGCTATGCACGA+AGG 0.621604 4:-98405170 None:intergenic
ATTGCCAACTGATATGAGAC+CGG 0.622238 4:+98408417 MsaT024337.1:CDS
GTACAATTAAGCAGTCACTC+AGG 0.623035 4:+98408090 MsaT024337.1:CDS
GTGCAAGAGTGGTGTGATCA+CGG 0.623081 4:+98410747 MsaT024337.1:CDS
TCAACGCTATTTGAACTGCA+GGG 0.626482 4:-98409587 None:intergenic
TGGTGCCAAAGTTGGCACAA+AGG 0.626604 4:+98408137 MsaT024337.1:CDS
GACACAGCTGTACCGCCTGA+CGG 0.626722 4:+98409299 MsaT024337.1:intron
ATTAAGCAGTCACTCAGGAG+GGG 0.627001 4:+98408095 MsaT024337.1:CDS
GCAAACCTCCTAATACATCC+GGG 0.627613 4:+98410279 MsaT024337.1:CDS
ATGATTGCAGAGATGATGAC+TGG 0.628430 4:+98402302 MsaT024337.1:intron
CAGATGGCAATTCAATAAAG+GGG 0.633431 4:+98404843 MsaT024337.1:CDS
AAAAGAAAGAAAACATGACT+GGG 0.634095 4:+98407371 MsaT024337.1:CDS
AGTTTATAGAGGATTTGTTG+CGG 0.634669 4:-98407486 None:intergenic
TGAGCCATGTAAGTTAATGT+CGG 0.634802 4:+98405048 MsaT024337.1:CDS
CAAGACTATTAAAGCAACAG+AGG 0.636817 4:+98407987 MsaT024337.1:CDS
CCTTTAGGGCTGCTATAACA+GGG 0.637214 4:+98407545 MsaT024337.1:CDS
ACTGTGTCACCGATCATGAA+GGG 0.639740 4:+98405106 MsaT024337.1:CDS
AAGTGGGCAGAGATTAGTGA+TGG 0.639850 4:-98401583 None:intergenic
GGTGAATTACAATCATTACA+TGG 0.640283 4:+98410119 MsaT024337.1:CDS
AATGGTGCATTACCTTCCAA+AGG 0.646787 4:-98405531 None:intergenic
TGTGTGGGATTTGATACTGA+AGG 0.648912 4:+98403737 MsaT024337.1:CDS
GTAATGAAAAGGAAGTGTCA+GGG 0.649835 4:+98409670 MsaT024337.1:CDS
ATTCCAGAAACAAGTGAGAG+AGG 0.650251 4:+98404989 MsaT024337.1:CDS
ATGGATATGGTTATTGACAG+GGG 0.650966 4:+98401274 MsaT024337.1:CDS
GCTACAGCTTACCTGCCTCA+CGG 0.652429 4:-98408476 None:intergenic
GGACCGATGAAAATATTCGA+AGG 0.652969 4:+98410300 MsaT024337.1:CDS
GGATCAACAGCACCTTCACA+TGG 0.653448 4:-98410029 None:intergenic
ATCACACCACTCTTGCACAA+TGG 0.654773 4:-98410742 None:intergenic
TCACGGCTGAACAGTACAGG+TGG 0.655166 4:+98410764 MsaT024337.1:CDS
AGATCCCCATCAACTTCAGG+AGG 0.655605 4:-98405019 None:intergenic
ACAGGTAGAAGCCTACATCA+AGG 0.658247 4:+98410708 MsaT024337.1:intron
CACGGCTGAACAGTACAGGT+GGG 0.658297 4:+98410765 MsaT024337.1:CDS
AGATGGCAATTCAATAAAGG+GGG 0.669533 4:+98404844 MsaT024337.1:CDS
AAGTACGTGAAAATCTAACA+AGG 0.671379 4:+98407641 MsaT024337.1:CDS
ACGAACCGTAAGCTTTCCAA+GGG 0.671646 4:+98407313 MsaT024337.1:CDS
AGTTTGCAAGGGTGATGATG+AGG 0.672466 4:-98409526 None:intergenic
ATTACATGGAGAGGGCAAGA+CGG 0.675282 4:+98410133 MsaT024337.1:CDS
AGGAGTCCAGAAGAAACGGA+AGG 0.678183 4:-98410005 None:intergenic
AAGCTTCAACCGATAGTTCG+GGG 0.688218 4:-98407135 None:intergenic
TTGTGATGACAGTGCCAGAG+TGG 0.696980 4:+98405715 MsaT024337.1:CDS
TGAGAAAGTAAAGAAACTCG+CGG 0.702852 4:+98410864 MsaT024337.1:CDS
ATAAAATTCCTGTATCCGTG+AGG 0.706166 4:+98408461 MsaT024337.1:CDS
AAGGTACATCTGACAGTACG+TGG 0.709384 4:+98403756 MsaT024337.1:CDS
AATGTTTAGAGCTAATGACT+CGG 0.710307 4:+98407189 MsaT024337.1:CDS
AAAGTAGATAAGATTGTACG+TGG 0.730669 4:+98408054 MsaT024337.1:CDS
GAAAACAATGAAGTGCCAGG+AGG 0.758156 4:+98409909 MsaT024337.1:CDS
TGATACGGGAGAGACAGCTG+TGG 0.814386 4:+98404937 MsaT024337.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 98401017 98410927 98401017 ID=MsaG024337
Chr4 mRNA 98401017 98410927 98401017 ID=MsaT024337.1;Parent=MsaG024337
Chr4 exon 98401017 98401091 98401017 ID=MsaT024337.1.exon1;Parent=MsaT024337.1
Chr4 CDS 98401017 98401091 98401017 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98401235 98401320 98401235 ID=MsaT024337.1.exon2;Parent=MsaT024337.1
Chr4 CDS 98401235 98401320 98401235 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98401551 98401641 98401551 ID=MsaT024337.1.exon3;Parent=MsaT024337.1
Chr4 CDS 98401551 98401641 98401551 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98401870 98401919 98401870 ID=MsaT024337.1.exon4;Parent=MsaT024337.1
Chr4 CDS 98401870 98401919 98401870 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98402312 98402381 98402312 ID=MsaT024337.1.exon5;Parent=MsaT024337.1
Chr4 CDS 98402312 98402381 98402312 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98403087 98403196 98403087 ID=MsaT024337.1.exon6;Parent=MsaT024337.1
Chr4 CDS 98403087 98403196 98403087 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98403724 98403789 98403724 ID=MsaT024337.1.exon7;Parent=MsaT024337.1
Chr4 CDS 98403724 98403789 98403724 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98404814 98405337 98404814 ID=MsaT024337.1.exon8;Parent=MsaT024337.1
Chr4 CDS 98404814 98405337 98404814 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98405460 98405540 98405460 ID=MsaT024337.1.exon9;Parent=MsaT024337.1
Chr4 CDS 98405460 98405540 98405460 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98405693 98405768 98405693 ID=MsaT024337.1.exon10;Parent=MsaT024337.1
Chr4 CDS 98405693 98405768 98405693 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98407093 98408308 98407093 ID=MsaT024337.1.exon11;Parent=MsaT024337.1
Chr4 CDS 98407093 98408308 98407093 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98408412 98408486 98408412 ID=MsaT024337.1.exon12;Parent=MsaT024337.1
Chr4 CDS 98408412 98408486 98408412 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98409300 98410385 98409300 ID=MsaT024337.1.exon13;Parent=MsaT024337.1
Chr4 CDS 98409300 98410385 98409300 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Chr4 exon 98410712 98410927 98410712 ID=MsaT024337.1.exon14;Parent=MsaT024337.1
Chr4 CDS 98410712 98410927 98410712 ID=cds.MsaT024337.1;Parent=MsaT024337.1
Gene Sequence

>MsaT024337.1

ATGGAATCAGATTTCGAGACGAACGATGATATGCTGACACTCTCACAGGATCCATTTGATGATGCCAACGACAATGATAATTTGGACGTTGAAGGGGAAACATGTGGAATATGTATGGATATGGTTATTGACAGGGGAGTATTGGATTGCTGTCAGCACTGGTTCTGCTTTGTATGTATTGACAACTGGGCTACCATCACTAATCTCTGCCCACTTTGCCAGAATGAGTTTCAGTTGATCACTTGTGTTCCAGTATATGATACCATAGGGAGCAATAAAGTTGAAGATGGCTCATTCCTCAGAGATGATGACTGGTCGATTGACGGGAAGAATAACAGTCTGTCATTTCCCTCTTACTACATTGATGAAAATGCAGTTACTTGCTTGGATGGTGATGGCTGCAAAATTCGAAATGGATTTGCAAGTGTTGAAGAAGATTCTGGTCTTGATACATCGATTGCTTGCGATTCATGTGATCTATGGTATCACGCCTTTTGTGTGGGATTTGATACTGAAGGTACATCTGACAGTACGTGGTTATGCCCAAGATGTGTGGTTGATGCAGTTTCTAAAGAAACAGATGGCAATTCAATAAAGGGGGCTTCCCTGGAATTTAACCCAGATAATACTAACAGTGAATGTCATGCCGGAATAGTGTCTGTGTCCATTGCTGATACGGGAGAGACAGCTGTGGTTGTCTCAATGGTTGACCGAAACAAATGGATTCCAGAAACAAGTGAGAGAGGCCTATTGCCTCCTGAAGTTGATGGGGATCTGTTGAATGAGCCATGTAAGTTAATGTCGGACACAAATAATCAGCTGCAGGCAACAGAGAAGACAACTGTGTCACCGATCATGAAGGGAGAAGAACTAGAGCTTTCATTATCACACCCAACTTCTAAATCCTTCGTGCATAGCGATTTAAAGGAAAGTGATAATGGAACAAGACGTGAACCGAGTAGCTTTGATGGAACCAAGTTATTTGATGAGTCCAATGCCAAAACTAGTCCATGCAAAATTGAATCTGATATGGGTCTTCATCTTGGTTTATCGGTGGGCACTTTTTTAACTGTCGATAATGTGGACAAGAGCGAATCAAAAGATCAAGCAACTGATCTCCCGTGCTCGATTCCAGAAGACTTTCCTTTGGAAGGAGATAAACTCGAAATCAATGCTTGTGATGACAGTGCCAGAGTGGCTGGTAGAAAGAGGAAGCATTTTGATTACAGTCATGAGCAAGTTCGTATAAAAGTTGAGGATGAAGATGCCAACCCCGAACTATCGGTTGAAGCTTCACAAAAGAAAACCAGAGCAGCTGCTAGTCAAATGTTTAGAGCTAATGACTCGGTCGATGTTCAACTTTCTGACAATGCTAAGAAAAGTCCCATTTTAAAACATTCTCCAACAAAGGAGATTGCAGCATCAGATATAATGAATATAGTTAAAGGGACGAACCGTAAGCTTTCCAAGGGACTTGCTGACACTAATGCTAATAAAAAGTTAGCTGAAAAGAAAGAAAACATGACTGGGTTGAGGGTGAAAAAGATTATGAAGAGAGTTTCTGATGATGGAGAATCATCCTCACTAGTCCAAAATCTAAGGAAGGAAATTAGAGAAGCTGTCCGCAACAAATCCTCTATAAACTTTGAGGAAAACCATTTTGATAAAAATCTTCTCGAAGCCTTTAGGGCTGCTATAACAGGGCCTAAAACTGAACTTGTAAATAAGTTATCCCCTTCAGCTCTGAAGGCAAAGAAGTCAATGTTGCAGAAAGGAAAAGTACGTGAAAATCTAACAAGGAAAATTTTTGGGACATCCAATGGTAGAAGAAAGCGTGCATGGGATAGGGATTGTGAAATTGAGTTTTGGAAATATCGTTGCATGAGAGCTTCAAAACCTGAAAAGGTCGAAACTTTAAAATCAGTTCTTGATCTTCTAAGAAAAAGTTCAGAGAACCCAGAATCAGAGCGAGCTCCCGAATGTCAGGCCAAGAATCCAATTCTTTCCAGATTGTATATAGCAGATACATCTGTTTTCCCACGGAAAGAGGATGTCAAACCTTTCTCGGAACAGACTAAACAAAATAATCCGTCAGCAAAAGGACCAAATCAATCTGTTGATGACAAGACTATTAAAGCAACAGAGGTAAATAATCTCTTATCAATGACCAAAGTTTGTTTATCTGAGAAAAAAGTAGATAAGATTGTACGTGGTCCAGCTGGTAAAGTACAATTAAGCAGTCACTCAGGAGGGGCATCAGTTTCTTCTTCAGCTGGTGCCAAAGTTGGCACAAAGGAATCTGGCCTTAAATCTGATTCTGTGAAAGGTGATAAAAGAAAATGGGCCTTGGAGGTTCTTGCGAGAAAAACAGCTGTTGCAAGTAAGAACACAGCAAATGAAAGTCAGGAAGACGATGCAATTTTTAAGGGAAATTATCCTTTGCTTGCCCAATTGCCAACTGATATGAGACCGGTGTTGGCACCTTGTCGCCACAATAAAATTCCTGTATCCGTGAGGCAGACACAGCTGTACCGCCTGACGGAACGCTTATTGAAGAACACAAATCTGCCAATCATTCGTAGAACTGCAGACACAGAGTTGGCTGTTGCAGATGCTATTAATATTGAAAAGGAGGTTGCTGACAGATCAAAAAGCAAGCTAGTTTATCTGAATCTTTGTTCACAAGAGCTACTGCATCGCACAAATAACACAAAATCCAACATAAACACAGACACAACTCTACCAACCTCATCATCACCCTTGCAAACTGATCAATCAGAACTAAATACTGATGATCTTTTGACTGACCCTGCAGTTCAAATAGCGTTGAAAAATGCTGGTTTGTTTTCTGATTCACCTCCAAGCAGTCCCCACAAGAACAATGAAATATGTAATGAAAAGGAAGTGTCAGGGCCTGATGATATACTTGAACTAGAATCTCATCCCGAGCTGGATATTTTTGGTGATTTTGAGTATGATTTGGAAGATGATGATTACATAGGTGCCAGTGTTACAAAGATCCCTAACCTAAAAGAAGAGCAGAGTGAATCAAAAGTGAAACTCGTTTTCTCCACCACGAGCTTGAAGAAAACAAATAATGCTTTGGATAGTGCAGACTGTAAGGGGTCTGAAAACAATGAAGTGCCAGGAGGTGCCTCTTGCTCACCAAACTGTCACAGAGATGCAGTCCAGAAGGATTCCACCATTAATGCAGAGATTGGCCAGCCTTCCGTTTCTTCTGGACTCCTACCATGTGAAGGTGCTGTTGATCCAGTTGATTCAGAATTTGAAGATTTATACGGCCCTGATAAAGAACCACTTATAAAAAAGTTTCCAGATGGTGAATTACAATCATTACATGGAGAGGGCAAGACGGAAACTCAATGCGAGAATAATGATTGCCATAAGGAAGAAGAACGCGACTTAGACAAAGCAGTAAATGGTGCTGAAGTAGGGAATGAGAATCTCACAGAAAATGTGTTTGTCCCTACCATCACTAGCAAACCTCCTAATACATCCGGGACCGATGAAAATATTCGAAGGAAGGAAGAGAGATCTGACATCCCTGCCCAGAAGTCAAACAATGAAAATCAAGTAGTGAAGAAGGTAGAAGCCTACATCAAGGAGCATATCAGGCCATTGTGCAAGAGTGGTGTGATCACGGCTGAACAGTACAGGTGGGCTGTTGCAAAAACAACTGAAAAGGTTATGAAGTATCATTGCAAATCAAAGAATGCTAATTTCCTCATAAAGGAAGGTGAGAAAGTAAAGAAACTCGCGGAGCAGTATGTTGAAACAGCCCAACAAAATAGAAAAAGTTGA
Protein sequence

>MsaT024337.1

MESDFETNDDMLTLSQDPFDDANDNDNLDVEGETCGICMDMVIDRGVLDCCQHWFCFVCIDNWATITNLCPLCQNEFQLITCVPVYDTIGSNKVEDGSFLRDDDWSIDGKNNSLSFPSYYIDENAVTCLDGDGCKIRNGFASVEEDSGLDTSIACDSCDLWYHAFCVGFDTEGTSDSTWLCPRCVVDAVSKETDGNSIKGASLEFNPDNTNSECHAGIVSVSIADTGETAVVVSMVDRNKWIPETSERGLLPPEVDGDLLNEPCKLMSDTNNQLQATEKTTVSPIMKGEELELSLSHPTSKSFVHSDLKESDNGTRREPSSFDGTKLFDESNAKTSPCKIESDMGLHLGLSVGTFLTVDNVDKSESKDQATDLPCSIPEDFPLEGDKLEINACDDSARVAGRKRKHFDYSHEQVRIKVEDEDANPELSVEASQKKTRAAASQMFRANDSVDVQLSDNAKKSPILKHSPTKEIAASDIMNIVKGTNRKLSKGLADTNANKKLAEKKENMTGLRVKKIMKRVSDDGESSSLVQNLRKEIREAVRNKSSINFEENHFDKNLLEAFRAAITGPKTELVNKLSPSALKAKKSMLQKGKVRENLTRKIFGTSNGRRKRAWDRDCEIEFWKYRCMRASKPEKVETLKSVLDLLRKSSENPESERAPECQAKNPILSRLYIADTSVFPRKEDVKPFSEQTKQNNPSAKGPNQSVDDKTIKATEVNNLLSMTKVCLSEKKVDKIVRGPAGKVQLSSHSGGASVSSSAGAKVGTKESGLKSDSVKGDKRKWALEVLARKTAVASKNTANESQEDDAIFKGNYPLLAQLPTDMRPVLAPCRHNKIPVSVRQTQLYRLTERLLKNTNLPIIRRTADTELAVADAINIEKEVADRSKSKLVYLNLCSQELLHRTNNTKSNINTDTTLPTSSSPLQTDQSELNTDDLLTDPAVQIALKNAGLFSDSPPSSPHKNNEICNEKEVSGPDDILELESHPELDIFGDFEYDLEDDDYIGASVTKIPNLKEEQSESKVKLVFSTTSLKKTNNALDSADCKGSENNEVPGGASCSPNCHRDAVQKDSTINAEIGQPSVSSGLLPCEGAVDPVDSEFEDLYGPDKEPLIKKFPDGELQSLHGEGKTETQCENNDCHKEEERDLDKAVNGAEVGNENLTENVFVPTITSKPPNTSGTDENIRRKEERSDIPAQKSNNENQVVKKVEAYIKEHIRPLCKSGVITAEQYRWAVAKTTEKVMKYHCKSKNANFLIKEGEKVKKLAEQYVETAQQNRKS*