Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024340 | KHN06707.1 | 86.667 | 60 | 8 | 0 | 1 | 60 | 103 | 162 | 4.34e-31 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024340 | sp|Q9C9F5|C3H15_ARATH | 83.636 | 55 | 9 | 0 | 3 | 57 | 217 | 271 | 1.85e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024340 | tr|A0A072VQ63|A0A072VQ63_MEDTR | 85.000 | 60 | 9 | 0 | 1 | 60 | 266 | 325 | 4.05e-31 | 120 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG024340 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG024340 | MsaG026339 | 0.932254 | 3.109312e-94 | 1.523972e-89 |
| MsaG024340 | MsaG026340 | 0.900426 | 1.708949e-77 | 8.967450e-74 |
| MsaG024340 | MsaG013377 | 0.801737 | 1.311096e-48 | 1.843698e-46 |
| MsaG024340 | MsaG023319 | 0.850903 | 2.398059e-60 | 1.340842e-57 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024340 | MtrunA17_Chr1g0209041 | 85.000 | 60 | 9 | 0 | 1 | 60 | 266 | 325 | 7.79e-35 | 120 |
| MsaG024340 | MtrunA17_Chr5g0442111 | 68.519 | 54 | 8 | 1 | 7 | 60 | 24 | 68 | 1.22e-19 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024340 | AT1G68200.2 | 83.636 | 55 | 9 | 0 | 3 | 57 | 216 | 270 | 1.56e-30 | 108 |
| MsaG024340 | AT1G68200.6 | 83.636 | 55 | 9 | 0 | 3 | 57 | 216 | 270 | 1.56e-30 | 108 |
| MsaG024340 | AT1G68200.1 | 83.636 | 55 | 9 | 0 | 3 | 57 | 217 | 271 | 1.88e-30 | 108 |
| MsaG024340 | AT1G68200.5 | 83.636 | 55 | 9 | 0 | 3 | 57 | 217 | 271 | 1.88e-30 | 108 |
| MsaG024340 | AT1G66810.1 | 72.222 | 54 | 15 | 0 | 3 | 56 | 227 | 280 | 7.34e-24 | 90.9 |
| MsaG024340 | AT1G66810.3 | 72.222 | 54 | 15 | 0 | 3 | 56 | 227 | 280 | 7.34e-24 | 90.9 |
Find 21 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAATTCGGTCACGACATT+GGG | 0.357280 | 4:-98425568 | MsaT024340.1:CDS |
| GAAAGTGGCACATGCCCTTA+TGG | 0.379603 | 4:-98425599 | MsaT024340.1:CDS |
| TATGGAGACCACTGTCAATT+CGG | 0.443498 | 4:-98425581 | MsaT024340.1:CDS |
| AATTGACAGTGGTCTCCATA+AGG | 0.458812 | 4:+98425584 | None:intergenic |
| TAGCGTGTGTGGCGGATCAC+TGG | 0.477964 | 4:+98425536 | None:intergenic |
| TGTCAATTCGGTCACGACAT+TGG | 0.489982 | 4:-98425569 | MsaT024340.1:CDS |
| GGTCCTTGCTTGTGTTGTGA+TGG | 0.515967 | 4:-98425492 | MsaT024340.1:CDS |
| AGACTGAGCTCTATAACAAA+TGG | 0.516543 | 4:-98425625 | MsaT024340.1:CDS |
| ATTGACAGTGGTCTCCATAA+GGG | 0.520085 | 4:+98425585 | None:intergenic |
| CTTGTGTTGTGATGGAGTGA+TGG | 0.547288 | 4:-98425484 | None:intergenic |
| GCTCAGTCTTGAACATCCCC+TGG | 0.557484 | 4:+98425639 | None:intergenic |
| TATAACAAATGGCAGGAAAG+TGG | 0.573891 | 4:-98425614 | MsaT024340.1:CDS |
| TGAGCTCTATAACAAATGGC+AGG | 0.577438 | 4:-98425621 | MsaT024340.1:CDS |
| CAAAACTGAGGTCTGCAAGA+TGG | 0.578924 | 4:-98425513 | MsaT024340.1:CDS |
| TGAGATGATTGTGTACAACC+AGG | 0.606228 | 4:-98425657 | None:intergenic |
| GAGATGATTGTGTACAACCA+GGG | 0.627071 | 4:-98425656 | MsaT024340.1:CDS |
| TCAATTCGGTCACGACATTG+GGG | 0.661919 | 4:-98425567 | MsaT024340.1:CDS |
| ACTCCATCACAACACAAGCA+AGG | 0.664459 | 4:+98425489 | None:intergenic |
| TGTCGTGACCGAATTGACAG+TGG | 0.697203 | 4:+98425573 | None:intergenic |
| CCACACACGCTACAAAACTG+AGG | 0.721664 | 4:-98425525 | MsaT024340.1:CDS |
| AGATGATTGTGTACAACCAG+GGG | 0.741581 | 4:-98425655 | MsaT024340.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 98425487 | 98425675 | 98425487 | ID=MsaG024340 |
| Chr4 | mRNA | 98425487 | 98425675 | 98425487 | ID=MsaT024340.1;Parent=MsaG024340 |
| Chr4 | exon | 98425487 | 98425675 | 98425487 | ID=MsaT024340.1.exon1;Parent=MsaT024340.1 |
| Chr4 | CDS | 98425487 | 98425675 | 98425487 | ID=cds.MsaT024340.1;Parent=MsaT024340.1 |
| Gene Sequence |
| Protein sequence |
