AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG024344

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024344 MtrunA17_Chr8g0388451 98.274 869 15 0 1 869 1 869 0.0 1784
MsaG024344 MtrunA17_Chr3g0102561 87.805 82 7 3 165 245 17 96 3.89e-33 124
MsaG024344 MtrunA17_Chr3g0104521 30.855 269 166 7 493 754 80 335 3.09e-27 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024344 AT4G14700.1 66.086 746 209 10 130 869 81 788 0.0 973
MsaG024344 AT4G12620.1 70.606 677 180 6 197 869 131 792 0.0 961
MsaG024344 AT1G07270.2 28.707 317 210 10 490 799 110 417 1.89e-30 126
MsaG024344 AT1G07270.1 28.707 317 210 10 490 799 110 417 2.62e-30 126
MsaG024344 AT2G29680.2 27.469 324 207 11 489 799 112 420 1.23e-26 115
MsaG024344 AT2G29680.1 25.581 344 197 12 489 788 112 440 3.04e-21 99.4

Find 214 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGATGAATCTGGCGATTT+TGG 0.133439 4:+98467745 None:intergenic
GTTAGTTACCGACTGGTTTC+TGG 0.202474 4:+98464200 None:intergenic
TGAAGCTGCTGATAATTATA+TGG 0.208304 4:+98463782 None:intergenic
CTGCACAAGGGACGGTTCTT+TGG 0.215974 4:-98465014 MsaT024344.1:CDS
CGCCCAACAGAAGTCTCTTC+TGG 0.247491 4:+98466989 None:intergenic
TCATCGGTTTGCATCATTAA+TGG 0.257487 4:+98460720 None:intergenic
CTCTATATTCACGGTGTTCC+GGG 0.266627 4:-98464709 MsaT024344.1:CDS
TGGCAGCTGAAGAAGTAATA+AGG 0.285779 4:+98460740 None:intergenic
CCTCTATATTCACGGTGTTC+CGG 0.288739 4:-98464710 MsaT024344.1:CDS
GTCATATATGAAGCATTAAA+TGG 0.289669 4:-98464348 MsaT024344.1:CDS
CTTCAGGTTGCAGCTATATC+AGG 0.290038 4:-98463578 MsaT024344.1:intron
TTTACAGATTTCGATGAATC+TGG 0.295754 4:+98467736 None:intergenic
AACCTACATTCCTCCACTTC+TGG 0.299054 4:+98467418 None:intergenic
CCGATTCTTCTCCCTCTTCT+CGG 0.303926 4:+98467928 None:intergenic
CTAGCTTTGGCATACTCCTT+AGG 0.308499 4:+98465528 None:intergenic
ATCTCCCTCATTGCTAGCTT+TGG 0.313313 4:+98465515 None:intergenic
TTCTTACCCTTCGGCGGCTT+TGG 0.317699 4:+98467238 None:intergenic
GGAGATCAATGGTCTGAAAT+TGG 0.328776 4:-98464481 MsaT024344.1:CDS
GGGAGATTGAAGAAGCTTCT+TGG 0.333604 4:+98468089 None:intergenic
AAAGAATTCCAGAGCATATA+AGG 0.339598 4:-98464971 MsaT024344.1:CDS
ATACCTGCAGCAACATTATC+AGG 0.340644 4:+98463476 None:intergenic
TTTGTAGAAGGGAAGAAAAC+TGG 0.345764 4:-98464279 MsaT024344.1:CDS
CTAAATGAGAGGTTTGTAGA+AGG 0.346073 4:-98464291 MsaT024344.1:CDS
ATTGAGTGTGATAGTTGTTT+AGG 0.355173 4:-98467367 MsaT024344.1:CDS
TGAGATTCGGCAACGAGATT+GGG 0.367135 4:+98467967 None:intergenic
TTGATCTCCACGAAACAGTA+TGG 0.382365 4:+98464495 None:intergenic
CCACATATCCAATCCCCTTC+AGG 0.385042 4:+98467301 None:intergenic
GCTGAATTGAAGAAGAGAAA+TGG 0.386079 4:-98467685 MsaT024344.1:CDS
TAGCTTTGGCATACTCCTTA+GGG 0.393164 4:+98465529 None:intergenic
TTTCAACTCACTTGAATATG+AGG 0.394384 4:+98462102 None:intergenic
TTGAGATTCGGCAACGAGAT+TGG 0.395152 4:+98467966 None:intergenic
TATTTCTGACAATCAATGTC+TGG 0.396034 4:-98464740 MsaT024344.1:CDS
ATAAGTCTTACCCAACTTGC+AGG 0.400447 4:+98461046 None:intergenic
AGAGTGACGGTGACGAAGAC+TGG 0.402154 4:-98465237 MsaT024344.1:CDS
AGGAGATGCACGCCGTGCTT+TGG 0.412726 4:-98463558 MsaT024344.1:CDS
GGAGATTGAAGAAGCTTCTT+GGG 0.412877 4:+98468090 None:intergenic
CTAACAAGCTTCTTACCCTT+CGG 0.416592 4:+98467229 None:intergenic
GCTATATCGGACCAACGATT+TGG 0.417826 4:-98466942 MsaT024344.1:CDS
TGAGAATTGTGAACGGAGGT+TGG 0.424141 4:+98468056 None:intergenic
AAAACGGTTGTTACAAGAGC+TGG 0.428477 4:-98467568 MsaT024344.1:CDS
GAAGCTGCTGATAATTATAT+GGG 0.430667 4:+98463783 None:intergenic
ATTTGAGCAGAGAGCTATAT+CGG 0.433378 4:-98466955 MsaT024344.1:CDS
TTCTTTGGACTTCAGAAGAT+AGG 0.436400 4:-98464999 MsaT024344.1:CDS
ATGATATCCTCCTGCAAGTT+GGG 0.437262 4:-98461056 MsaT024344.1:intron
AGCAATGGTGAAGTGTTTCC+TGG 0.439086 4:-98461081 MsaT024344.1:CDS
GAGCTTTACAAAACAGGAAT+GGG 0.441059 4:-98461530 MsaT024344.1:CDS
TCCGACGTCGCCGGATCAAT+CGG 0.442884 4:-98467632 MsaT024344.1:CDS
GACGGCGGTGAATTTGAAGT+TGG 0.443655 4:-98467493 MsaT024344.1:CDS
GGGCGTTGCCTCTATATTCA+CGG 0.455755 4:-98464718 MsaT024344.1:CDS
GGCTTCAATCAAGTGATTTG+TGG 0.457739 4:-98467192 MsaT024344.1:CDS
CATTCCAAGCTAATTGTGAT+AGG 0.457837 4:-98464054 MsaT024344.1:intron
ATCATTTCGAGTCGCCTCAA+TGG 0.464299 4:-98463755 MsaT024344.1:CDS
ACGGCGGTGAATTTGAAGTT+GGG 0.465132 4:-98467492 MsaT024344.1:CDS
CTTACCTATCACAATTAGCT+TGG 0.465486 4:+98464050 None:intergenic
AAGATGTTCCTGAAGGGGAT+TGG 0.466368 4:-98467309 MsaT024344.1:CDS
TCGGAGCGAATGAAACCTTA+AGG 0.466715 4:+98467650 None:intergenic
GGCAGCTGAAGAAGTAATAA+GGG 0.468817 4:+98460741 None:intergenic
GTGCACGAGCTTTACAAAAC+AGG 0.469313 4:-98461536 MsaT024344.1:CDS
TGATTGTTGAATTTGAGATT+CGG 0.470559 4:+98467954 None:intergenic
CATCAAACCATACTGTTTCG+TGG 0.472974 4:-98464502 MsaT024344.1:CDS
TTTCTACAAGGTAAAGACTT+AGG 0.474900 4:+98464891 None:intergenic
TTGTGAGACCTTATATGCTC+TGG 0.474919 4:+98464963 None:intergenic
TTCTTAGTTTCCGATTGATC+CGG 0.476110 4:+98467622 None:intergenic
CGAGGTCAAGGTTGAGAAAA+CGG 0.476473 4:-98467584 MsaT024344.1:CDS
GCCCAACAGAAGTCTCTTCT+GGG 0.476671 4:+98466990 None:intergenic
ATACGAGGAAGTAACTTCTC+TGG 0.476939 4:+98463842 None:intergenic
GCTTCAATCAAGTGATTTGT+GGG 0.480427 4:-98467191 MsaT024344.1:CDS
CCTGAAGGGGATTGGATATG+TGG 0.480446 4:-98467301 MsaT024344.1:CDS
TGGGAAGGGAGATTCCCTTA+AGG 0.483741 4:-98467665 MsaT024344.1:CDS
CTGAATTGAAGAAGAGAAAT+GGG 0.485254 4:-98467684 MsaT024344.1:CDS
GAGTGTGATAGTTGTTTAGG+TGG 0.490466 4:-98467364 MsaT024344.1:CDS
TCATGTCTCTGCGGCAGCAA+TGG 0.493304 4:-98461096 MsaT024344.1:CDS
TGGTCTGAAATTGGCTTCAC+CGG 0.494433 4:-98464472 MsaT024344.1:CDS
TTCTGTACAATATTCTTGAC+TGG 0.495175 4:-98464089 MsaT024344.1:CDS
GCAGCTACTGGTGTCGGGTA+CGG 0.495197 4:-98467024 MsaT024344.1:CDS
TAAATGAGAGGTTTGTAGAA+GGG 0.497183 4:-98464290 MsaT024344.1:CDS
ATTCACGGTGTTCCGGGCAC+TGG 0.498438 4:-98464703 MsaT024344.1:CDS
AACAATGCAGTTGGCAATGA+CGG 0.500318 4:-98461121 MsaT024344.1:intron
GTGGCACAGTTTCAAACGCT+TGG 0.500569 4:-98465457 MsaT024344.1:CDS
GTTACAAGAGCTGGGAGAAA+AGG 0.500757 4:-98467559 MsaT024344.1:CDS
AAAGCCTCTGGATGCCCATT+CGG 0.503391 4:+98463813 None:intergenic
GGATGGCAGCTACTGGTGTC+GGG 0.503839 4:-98467029 MsaT024344.1:CDS
TTCACCTTGTAAATATTCTC+CGG 0.504027 4:+98464453 None:intergenic
CCGAGAAGAGGGAGAAGAAT+CGG 0.504286 4:-98467928 MsaT024344.1:CDS
GCAGCTAACTGATGGCTTGT+CGG 0.504655 4:+98465130 None:intergenic
TTCATTCGCTCCGACGTCGC+CGG 0.505449 4:-98467641 MsaT024344.1:CDS
ATCTGTGAGGGTCGTAAGAT+GGG 0.506846 4:-98467277 MsaT024344.1:CDS
CGGAGCGAATGAAACCTTAA+GGG 0.510307 4:+98467651 None:intergenic
CCTCGTATATCAAGCCGAAT+GGG 0.511380 4:-98463827 MsaT024344.1:CDS
GATAGTACCTTGCCAGTGCC+CGG 0.512164 4:+98464691 None:intergenic
ATCCCAGAAGAGACTTCTGT+TGG 0.512492 4:-98466992 MsaT024344.1:CDS
TCAAGACGAGATTTCGCAGC+TGG 0.512532 4:+98467892 None:intergenic
CGGAAACTAAGAAGAGAAAG+AGG 0.512657 4:-98467612 MsaT024344.1:CDS
GATCTGTGAGGGTCGTAAGA+TGG 0.515072 4:-98467278 MsaT024344.1:CDS
TTGAAGTCAGAAGTTGATGC+AGG 0.515204 4:-98464528 MsaT024344.1:CDS
ATTTCTGACAATCAATGTCT+GGG 0.516051 4:-98464739 MsaT024344.1:CDS
AGCTTGGAATGTGGCTTGGT+TGG 0.517458 4:+98464066 None:intergenic
CAAATATATCAATTCCATTG+AGG 0.517649 4:+98463741 None:intergenic
TGGAGCAAATGCAATTTCAT+CGG 0.518476 4:+98467765 None:intergenic
TACTGTTTCGTGGAGATCAA+TGG 0.518500 4:-98464492 MsaT024344.1:CDS
GATCCTGATAATGTTGCTGC+AGG 0.521500 4:-98463479 MsaT024344.1:intron
TACAAGAAAGTTGTTTATGA+CGG 0.522606 4:-98467511 MsaT024344.1:CDS
GCATTAAATGGACACAGGGT+TGG 0.523201 4:-98464336 MsaT024344.1:CDS
GGTAAGGAATGAGGAGTGAG+AGG 0.524682 4:+98468033 None:intergenic
CAAACCTCTCATTTAGCAAA+CGG 0.525477 4:+98464298 None:intergenic
ATGGGGCAAAGCAAAGCCTC+TGG 0.529383 4:+98463801 None:intergenic
AGCGTTTGAAACTGTGCCAC+TGG 0.529464 4:+98465460 None:intergenic
TCAACATACTTATTTCTACA+AGG 0.530757 4:+98464879 None:intergenic
TTGAAGAAGAGAAATGGGAA+GGG 0.532289 4:-98467679 MsaT024344.1:CDS
GCTCGTGCACCATAGCTGTC+AGG 0.532743 4:+98461549 None:intergenic
CGTGGATCTACGTGTAGGTA+AGG 0.533392 4:+98468017 None:intergenic
CTCGATGTTGGCCAAATCGT+TGG 0.533909 4:+98466931 None:intergenic
TGGATGGCAGCTACTGGTGT+CGG 0.533932 4:-98467030 MsaT024344.1:CDS
ATCTGTAAAGAAGAGGAAGC+AGG 0.534383 4:-98467722 MsaT024344.1:CDS
TCACTTGAATATGAGGTGCT+TGG 0.534848 4:+98462109 None:intergenic
CGAGCTTTACAAAACAGGAA+TGG 0.537464 4:-98461531 MsaT024344.1:CDS
AATTAGCTTGGAATGTGGCT+TGG 0.540690 4:+98464062 None:intergenic
CGGTGTTCCGGGCACTGGCA+AGG 0.541081 4:-98464698 MsaT024344.1:intron
ATCCAGAAGTGGAGGAATGT+AGG 0.542570 4:-98467420 MsaT024344.1:CDS
GAGATTCGGCAACGAGATTG+GGG 0.543738 4:+98467968 None:intergenic
TAAATGGACACAGGGTTGGA+TGG 0.543748 4:-98464332 MsaT024344.1:CDS
GTGAAGCAGGTGCCAAGCAC+AGG 0.543759 4:-98460807 MsaT024344.1:CDS
AGTGTGATAGTTGTTTAGGT+GGG 0.547500 4:-98467363 MsaT024344.1:CDS
CATGATATCCTCCTGCAAGT+TGG 0.553301 4:-98461057 MsaT024344.1:CDS
CCGGAACACCGTGAATATAG+AGG 0.553353 4:+98464710 None:intergenic
CATCGAAATCTGTAAAGAAG+AGG 0.553381 4:-98467729 MsaT024344.1:CDS
TCCTCGTATATCAAGCCGAA+TGG 0.555101 4:-98463828 MsaT024344.1:CDS
TTCTTGGGAGTTGAAGCCAT+TGG 0.556223 4:+98468105 None:intergenic
GGGATGATGTTTATGTGAAG+AGG 0.559397 4:-98467471 MsaT024344.1:CDS
AAAGAGGAAGAACGAGGTCA+AGG 0.561799 4:-98467596 MsaT024344.1:CDS
TGCAGGAGGATATCATGTCC+AGG 0.562882 4:+98461063 None:intergenic
AACAATCAACTCCGAGAAGA+GGG 0.566074 4:-98467939 MsaT024344.1:CDS
AGCTCTCTGCTCAAATTATG+TGG 0.566453 4:+98466962 None:intergenic
TTCATACTGCAGCTAACTGA+TGG 0.566543 4:+98465122 None:intergenic
ATTGAAGAAGAGAAATGGGA+AGG 0.568468 4:-98467680 MsaT024344.1:CDS
TTGGATATGTGGGATCTGTG+AGG 0.570339 4:-98467290 MsaT024344.1:CDS
ACAATCCATCACCTCGATGT+TGG 0.575437 4:+98466919 None:intergenic
CTCTCCGTTTGCTAAATGAG+AGG 0.575524 4:-98464302 MsaT024344.1:CDS
TGTGGGCTGCTCGCATTGAG+AGG 0.577481 4:-98467174 MsaT024344.1:intron
AGGGAAGAAAACTGGAGAAG+AGG 0.577588 4:-98464271 MsaT024344.1:CDS
TCCCAGAAGAGACTTCTGTT+GGG 0.577921 4:-98466991 MsaT024344.1:CDS
AAGAAGTGGATGGCAGCTAC+TGG 0.578137 4:-98467036 MsaT024344.1:CDS
GGGCTTTGAGAAATAACCTC+GGG 0.578651 4:+98460761 None:intergenic
CGTTGAAAGATGTTCCTGAA+GGG 0.581179 4:-98467315 MsaT024344.1:CDS
TCCGATTGATCCGGCGACGT+CGG 0.582582 4:+98467631 None:intergenic
TATGCCAAAGCTAGCAATGA+GGG 0.585809 4:-98465519 MsaT024344.1:CDS
TGAGAGGTGAGAATTGTGAA+CGG 0.585848 4:+98468049 None:intergenic
TTGCTATGTCATGATCCCTA+AGG 0.591823 4:-98465544 MsaT024344.1:CDS
TTGTTAGAACAATGCGTCAG+AGG 0.591921 4:-98467213 MsaT024344.1:CDS
CCGTTGAAAGATGTTCCTGA+AGG 0.592540 4:-98467316 MsaT024344.1:CDS
CTGAAGGGGATTGGATATGT+GGG 0.593658 4:-98467300 MsaT024344.1:CDS
ACACAGGGTTGGATGGAAAG+AGG 0.594099 4:-98464325 MsaT024344.1:CDS
AGGAAAGGGACTTGTTTGCA+TGG 0.594820 4:-98462163 MsaT024344.1:CDS
ACAAGCTTCTTACCCTTCGG+CGG 0.596059 4:+98467232 None:intergenic
CAACAATCAACTCCGAGAAG+AGG 0.596926 4:-98467940 MsaT024344.1:CDS
TAGCAGTATATGGAAAGAAG+TGG 0.597595 4:-98467050 MsaT024344.1:intron
AAACGGTTGTTACAAGAGCT+GGG 0.597984 4:-98467567 MsaT024344.1:CDS
GTTGAAAGATGTTCCTGAAG+GGG 0.598838 4:-98467314 MsaT024344.1:CDS
ATGAAGCATTAAATGGACAC+AGG 0.600808 4:-98464341 MsaT024344.1:CDS
TACAGAATCTGCACAAGGGA+CGG 0.603574 4:-98465022 MsaT024344.1:intron
GCTGTAAGTTGGGTGAGTGT+AGG 0.604738 4:-98460840 MsaT024344.1:CDS
GACTAATCTTGAAAGAGCAA+AGG 0.606188 4:-98464937 MsaT024344.1:CDS
GTCTGCATATTTCCAAAGCA+CGG 0.606454 4:+98463546 None:intergenic
TGGATATGTGGGATCTGTGA+GGG 0.607873 4:-98467289 MsaT024344.1:CDS
TTCACCGGAGAATATTTACA+AGG 0.608665 4:-98464457 MsaT024344.1:intron
TTCAGGGATAGCAAATACAA+TGG 0.610395 4:-98463870 MsaT024344.1:intron
AATTTCTGAAGAAGAGAAGT+CGG 0.611112 4:-98467798 MsaT024344.1:CDS
CGGCGGTGAATTTGAAGTTG+GGG 0.617237 4:-98467491 MsaT024344.1:CDS
CCTTCAGGAACATCTTTCAA+CGG 0.617438 4:+98467316 None:intergenic
AGAAGATGCTACTTCTGATG+AGG 0.621654 4:-98467446 MsaT024344.1:CDS
ACAAGAGCTGGGAGAAAAGG+TGG 0.622286 4:-98467556 MsaT024344.1:CDS
TCATGATTTCGTCCCCAGAA+CGG 0.622364 4:+98467389 None:intergenic
TCGTCAATGAGCAAAATACA+TGG 0.624876 4:+98464240 None:intergenic
TGAGGAAGATCCAGAAGTGG+AGG 0.628093 4:-98467428 MsaT024344.1:CDS
GTATGCCAAAGCTAGCAATG+AGG 0.629062 4:-98465520 MsaT024344.1:CDS
CTACGTGTAGGTAAGGAATG+AGG 0.635826 4:+98468024 None:intergenic
CAATGAGCAAAATACATGGT+CGG 0.635961 4:+98464244 None:intergenic
CCCATTCGGCTTGATATACG+AGG 0.638148 4:+98463827 None:intergenic
TGAAGCATTAAATGGACACA+GGG 0.640411 4:-98464340 MsaT024344.1:CDS
AGTATCGGAGTTCAAGCGCG+TGG 0.641574 4:+98467999 None:intergenic
AAGCGCGTGGATCTACGTGT+AGG 0.642274 4:+98468012 None:intergenic
GCTACTGGTGTCGGGTACGG+TGG 0.642777 4:-98467021 MsaT024344.1:CDS
GCTAACTGATGGCTTGTCGG+TGG 0.645325 4:+98465133 None:intergenic
TGATGAGGAAGATCCAGAAG+TGG 0.647235 4:-98467431 MsaT024344.1:CDS
AGGGCTTTGAGAAATAACCT+CGG 0.647966 4:+98460760 None:intergenic
ATCACAATTAGCTTGGAATG+TGG 0.648587 4:+98464057 None:intergenic
AGTATATGGAAAGAAGTGGA+TGG 0.649668 4:-98467046 MsaT024344.1:CDS
AGATTCGGCAACGAGATTGG+GGG 0.651554 4:+98467969 None:intergenic
TCATGACATAGCAATGTCGA+AGG 0.658256 4:+98465553 None:intergenic
AAGAAAGTTGTTTATGACGG+CGG 0.658330 4:-98467508 MsaT024344.1:CDS
GAGATAACGACATTCATCAA+AGG 0.658884 4:-98464766 MsaT024344.1:CDS
TGAGTGTACTCTCAGTGATG+AGG 0.663135 4:-98464554 MsaT024344.1:CDS
TCTTGTAACCAGAAACCAGT+CGG 0.664804 4:-98464208 MsaT024344.1:intron
CGAAGACTGGAACATCGACA+AGG 0.665993 4:-98465224 MsaT024344.1:CDS
AAGCTGCTGATAATTATATG+GGG 0.670932 4:+98463784 None:intergenic
GAGGTGAGAATTGTGAACGG+AGG 0.673704 4:+98468052 None:intergenic
TGACATTGATGATGAAAGAG+AGG 0.674539 4:-98465433 MsaT024344.1:intron
AGTCTTACCCAACTTGCAGG+AGG 0.676386 4:+98461049 None:intergenic
TCAGGAACATCTTTCAACGG+TGG 0.680289 4:+98467319 None:intergenic
GCTTTACAAAACAGGAATGG+GGG 0.682677 4:-98461528 MsaT024344.1:CDS
AGCTTTACAAAACAGGAATG+GGG 0.683635 4:-98461529 MsaT024344.1:CDS
AAAAGGTGGAAAGATTGCGA+AGG 0.684724 4:-98467542 MsaT024344.1:CDS
AATGATACAGAATCTGCACA+AGG 0.686949 4:-98465027 MsaT024344.1:intron
GTAAAGAAGAGGAAGCAGGA+AGG 0.690620 4:-98467718 MsaT024344.1:CDS
AGTTGCAGCTCAATTTCCCG+AGG 0.692840 4:-98460777 MsaT024344.1:CDS
TGAAGATGTTGAATATGATG+AGG 0.693036 4:-98465182 MsaT024344.1:CDS
CAACGATTTGGCCAACATCG+AGG 0.693338 4:-98466930 MsaT024344.1:intron
GAAGAGAAAGAGGAAGAACG+AGG 0.698607 4:-98467602 MsaT024344.1:CDS
TTTCTGACAATCAATGTCTG+GGG 0.698842 4:-98464738 MsaT024344.1:CDS
CAAGCCGAATGGGCATCCAG+AGG 0.706358 4:-98463817 MsaT024344.1:CDS
AATATGAATATGACATCCAG+TGG 0.707312 4:-98465476 MsaT024344.1:CDS
ATGACGGTTTCATGTCTCTG+CGG 0.719836 4:-98461105 MsaT024344.1:CDS
ATGATACAGAATCTGCACAA+GGG 0.728041 4:-98465026 MsaT024344.1:intron
TAGAATTCTGATGAGAGTGA+CGG 0.741239 4:-98465250 MsaT024344.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 98460720 98468124 98460720 ID=MsaG024344
Chr4 mRNA 98460720 98468124 98460720 ID=MsaT024344.1;Parent=MsaG024344
Chr4 exon 98460720 98460861 98460720 ID=MsaT024344.1.exon15;Parent=MsaT024344.1
Chr4 CDS 98460720 98460861 98460720 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98461057 98461133 98461057 ID=MsaT024344.1.exon14;Parent=MsaT024344.1
Chr4 CDS 98461057 98461133 98461057 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98461511 98461606 98461511 ID=MsaT024344.1.exon13;Parent=MsaT024344.1
Chr4 CDS 98461511 98461606 98461511 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98462113 98462183 98462113 ID=MsaT024344.1.exon12;Parent=MsaT024344.1
Chr4 CDS 98462113 98462183 98462113 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98463480 98463594 98463480 ID=MsaT024344.1.exon11;Parent=MsaT024344.1
Chr4 CDS 98463480 98463594 98463480 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98463697 98463887 98463697 ID=MsaT024344.1.exon10;Parent=MsaT024344.1
Chr4 CDS 98463697 98463887 98463697 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98464055 98464112 98464055 ID=MsaT024344.1.exon9;Parent=MsaT024344.1
Chr4 CDS 98464055 98464112 98464055 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98464209 98464370 98464209 ID=MsaT024344.1.exon8;Parent=MsaT024344.1
Chr4 CDS 98464209 98464370 98464209 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98464458 98464580 98464458 ID=MsaT024344.1.exon7;Parent=MsaT024344.1
Chr4 CDS 98464458 98464580 98464458 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98464699 98464798 98464699 ID=MsaT024344.1.exon6;Parent=MsaT024344.1
Chr4 CDS 98464699 98464798 98464699 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98464888 98465039 98464888 ID=MsaT024344.1.exon5;Parent=MsaT024344.1
Chr4 CDS 98464888 98465039 98464888 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98465129 98465269 98465129 ID=MsaT024344.1.exon4;Parent=MsaT024344.1
Chr4 CDS 98465129 98465269 98465129 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98465434 98465586 98465434 ID=MsaT024344.1.exon3;Parent=MsaT024344.1
Chr4 CDS 98465434 98465586 98465434 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98466931 98467066 98466931 ID=MsaT024344.1.exon2;Parent=MsaT024344.1
Chr4 CDS 98466931 98467066 98466931 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Chr4 exon 98467175 98468124 98467175 ID=MsaT024344.1.exon1;Parent=MsaT024344.1
Chr4 CDS 98467175 98468124 98467175 ID=cds.MsaT024344.1;Parent=MsaT024344.1
Gene Sequence

>MsaT024344.1

ATGGCTTCAACTCCCAAGAAGCTTCTTCAATCTCCCTCCTCAAAATCCAACCTCCGTTCACAATTCTCACCTCTCACTCCTCATTCCTTACCTACACGTAGATCCACGCGCTTGAACTCCGATACTCCCATAACCCCCAATCTCGTTGCCGAATCTCAAATTCAACAATCAACTCCGAGAAGAGGGAGAAGAATCGGTGAGAATTCTGTTCCAGCTGCGAAATCTCGTCTTGATTTCACTCGAAAAGATAAAGCTGTAACTCACAATAGTTCAATTAAAGTAGAAAATGAAATTGATTCTGCTCAATTTCTGAAGAAGAGAAGTCGGAAAGCTGAATCCGATGAAATTGCATTTGCTCCAAAATCGCCAGATTCATCGAAATCTGTAAAGAAGAGGAAGCAGGAAGGTGAAAAAACGGCTGAATTGAAGAAGAGAAATGGGAAGGGAGATTCCCTTAAGGTTTCATTCGCTCCGACGTCGCCGGATCAATCGGAAACTAAGAAGAGAAAGAGGAAGAACGAGGTCAAGGTTGAGAAAACGGTTGTTACAAGAGCTGGGAGAAAAGGTGGAAAGATTGCGAAGGTGCAGTATTACAAGAAAGTTGTTTATGACGGCGGTGAATTTGAAGTTGGGGATGATGTTTATGTGAAGAGGAGAGAAGATGCTACTTCTGATGAGGAAGATCCAGAAGTGGAGGAATGTAGGTTTTGTTTCCGTTCTGGGGACGAAATCATGATTGAGTGTGATAGTTGTTTAGGTGGGTTTCATTTGAAATGTTTGACACCACCGTTGAAAGATGTTCCTGAAGGGGATTGGATATGTGGGATCTGTGAGGGTCGTAAGATGGGTAAAGATGTTGATTTTCCAAAGCCGCCGAAGGGTAAGAAGCTTGTTAGAACAATGCGTCAGAGGCTTCAATCAAGTGATTTGTGGGCTGCTCGCATTGAGAGTATATGGAAAGAAGTGGATGGCAGCTACTGGTGTCGGGTACGGTGGTATATGATCCCAGAAGAGACTTCTGTTGGGCGACAACCACATAATTTGAGCAGAGAGCTATATCGGACCAACGATTTGGCCAACATCGAGATGGAATCTGTCCTTCGACATTGCTATGTCATGATCCCTAAGGAGTATGCCAAAGCTAGCAATGAGGGAGATGATGTTTTCTTGTGTGAATATGAATATGACATCCAGTGGCACAGTTTCAAACGCTTGGCTGACATTGATGATGAAAGAGAGAATTCTGATGAGAGTGACGGTGACGAAGACTGGAACATCGACAAGGAATCAGACTCTGACACAGATGAAGATGTTGAATATGATGAGGAAAATATTAAAATTTCACAATCGCAACCACCGACAAGCCATCAGTTAGCTGCAAATCTGCACAAGGGACGGTTCTTTGGACTTCAGAAGATAGGCACAAAAAGAATTCCAGAGCATATAAGGTCTCACAAACAGACTAATCTTGAAAGAGCAAAGGCATCATTGCTGTTAGCTTCGCTGCCTAAGTCTTTACCTTGTAGAAATAAAGAAATGGACGAGATAACGACATTCATCAAAGGTGCTATTTCTGACAATCAATGTCTGGGGCGTTGCCTCTATATTCACGGTGTTCCGGGCACTGGCAAGACAATGAGTGTACTCTCAGTGATGAGGAGTTTGAAGTCAGAAGTTGATGCAGGAAACATCAAACCATACTGTTTCGTGGAGATCAATGGTCTGAAATTGGCTTCACCGGAGAATATTTACAAGGTCATATATGAAGCATTAAATGGACACAGGGTTGGATGGAAAGAGGCTCTCCGTTTGCTAAATGAGAGGTTTGTAGAAGGGAAGAAAACTGGAGAAGAGGCTGACCGACCATGTATTTTGCTCATTGACGAACTTGATCTTCTTGTAACCAGAAACCAGTCGGTTCTGTACAATATTCTTGACTGGCCAACCAAGCCACATTCCAAGCTAATTGTGATAGGGATAGCAAATACAATGGATCTTCCAGAGAAGTTACTTCCTCGTATATCAAGCCGAATGGGCATCCAGAGGCTTTGCTTTGCCCCATATAATTATCAGCAGCTTCAAGAAATCATTTCGAGTCGCCTCAATGGAATTGATATATTTGAAAAACAAGCTATGGAATTTGCTTCAAGAAAGTTTAGTCTCCAAGTTGCAGCTATATCAGGAGATGCACGCCGTGCTTTGGAAATATGCAGACGTGCAGCTGAAATTGCAGATTATCGAATGAAGAAGCTAGCTTCAGATCCTGATAATGTTGCTGCAGGAAAGGGACTTGTTTGCATGGCAGACGTTGAATCAGCTATTCAAGAAATGTTCCAAGCACCTCATATTCAAGTGATGAAAAACTGTTCCAGACTTGGCAAAATTTTCCTGACAGCTATGGTGCACGAGCTTTACAAAACAGGAATGGGGGAAACCACTTTTGAAAAGTTGGCAATGACGGTTTCATGTCTCTGCGGCAGCAATGGTGAAGTGTTTCCTGGACATGATATCCTCCTGCAAGTTGGCTGTAAGTTGGGTGAGTGTAGGATCATTTTGTGTGAAGCAGGTGCCAAGCACAGGTTGCAAAAGTTGCAGCTCAATTTCCCGAGGTTATTTCTCAAAGCCCTTATTACTTCTTCAGCTGCCATTAATGATGCAAACCGATGA
Protein sequence

>MsaT024344.1

MASTPKKLLQSPSSKSNLRSQFSPLTPHSLPTRRSTRLNSDTPITPNLVAESQIQQSTPRRGRRIGENSVPAAKSRLDFTRKDKAVTHNSSIKVENEIDSAQFLKKRSRKAESDEIAFAPKSPDSSKSVKKRKQEGEKTAELKKRNGKGDSLKVSFAPTSPDQSETKKRKRKNEVKVEKTVVTRAGRKGGKIAKVQYYKKVVYDGGEFEVGDDVYVKRREDATSDEEDPEVEECRFCFRSGDEIMIECDSCLGGFHLKCLTPPLKDVPEGDWICGICEGRKMGKDVDFPKPPKGKKLVRTMRQRLQSSDLWAARIESIWKEVDGSYWCRVRWYMIPEETSVGRQPHNLSRELYRTNDLANIEMESVLRHCYVMIPKEYAKASNEGDDVFLCEYEYDIQWHSFKRLADIDDERENSDESDGDEDWNIDKESDSDTDEDVEYDEENIKISQSQPPTSHQLAANLHKGRFFGLQKIGTKRIPEHIRSHKQTNLERAKASLLLASLPKSLPCRNKEMDEITTFIKGAISDNQCLGRCLYIHGVPGTGKTMSVLSVMRSLKSEVDAGNIKPYCFVEINGLKLASPENIYKVIYEALNGHRVGWKEALRLLNERFVEGKKTGEEADRPCILLIDELDLLVTRNQSVLYNILDWPTKPHSKLIVIGIANTMDLPEKLLPRISSRMGIQRLCFAPYNYQQLQEIISSRLNGIDIFEKQAMEFASRKFSLQVAAISGDARRALEICRRAAEIADYRMKKLASDPDNVAAGKGLVCMADVESAIQEMFQAPHIQVMKNCSRLGKIFLTAMVHELYKTGMGETTFEKLAMTVSCLCGSNGEVFPGHDILLQVGCKLGECRIILCEAGAKHRLQKLQLNFPRLFLKALITSSAAINDANR*