Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024501 | XP_004503437.2 | 98.413 | 63 | 1 | 0 | 18 | 80 | 180 | 242 | 1.21e-33 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024501 | sp|Q3E9B4|OFP8_ARATH | 71.429 | 35 | 10 | 0 | 49 | 83 | 154 | 188 | 3.56e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024501 | tr|A0A1S2YE06|A0A1S2YE06_CICAR | 98.413 | 63 | 1 | 0 | 18 | 80 | 180 | 242 | 5.79e-34 | 127 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG024501 | TF | OFP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024501 | MtrunA17_Chr8g0390531 | 94.231 | 52 | 3 | 0 | 32 | 83 | 241 | 292 | 6.18e-29 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024501 | AT5G19650.1 | 71.429 | 35 | 10 | 0 | 49 | 83 | 154 | 188 | 3.62e-12 | 59.7 |
Find 17 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCACGCACGCACGTTTAA+AGG | 0.272537 | 4:+100187520 | MsaT024501.1:CDS |
| ATGTCGGCGGCGAGACTTAG+AGG | 0.357232 | 4:-100188416 | None:intergenic |
| AAACGTGCGTGCGTGACCAC+CGG | 0.443819 | 4:-100187515 | None:intergenic |
| CTTCTCGCCGGAAAACTGAC+CGG | 0.509033 | 4:+100188440 | MsaT024501.1:CDS |
| ATGGGTGTTCTTCCGTTGAA+CGG | 0.516099 | 4:+100188472 | MsaT024501.1:CDS |
| GAAGTTCTAAAATCATTGTA+CGG | 0.539349 | 4:-100188538 | None:intergenic |
| AACGTGCGTGCGTGACCACC+GGG | 0.546888 | 4:-100187514 | None:intergenic |
| GCCGCCGACATTCTTCTCGC+CGG | 0.549096 | 4:+100188428 | MsaT024501.1:CDS |
| TTAAAGGCAAAGGCAGAAGA+AGG | 0.559345 | 4:+100187536 | MsaT024501.1:CDS |
| AACTGACCGGAGTTCCGACA+TGG | 0.569932 | 4:+100188453 | MsaT024501.1:CDS |
| GAACACCCATGTCGGAACTC+CGG | 0.589971 | 4:-100188459 | None:intergenic |
| CAACGGAAGAACACCCATGT+CGG | 0.598602 | 4:-100188467 | None:intergenic |
| GCACGCACGTTTAAAGGCAA+AGG | 0.606289 | 4:+100187526 | MsaT024501.1:CDS |
| AAAAGTCAAAGACACTTTCG+CGG | 0.636990 | 4:+100188495 | MsaT024501.1:CDS |
| TCCGGCGAGAAGAATGTCGG+CGG | 0.657252 | 4:-100188429 | None:intergenic |
| ACTGACCGGAGTTCCGACAT+GGG | 0.670070 | 4:+100188454 | MsaT024501.1:CDS |
| AGTCAAAGACACTTTCGCGG+TGG | 0.716882 | 4:+100188498 | MsaT024501.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 100187506 | 100192337 | 100187506 | ID=MsaG024501 |
| Chr4 | mRNA | 100187506 | 100192337 | 100187506 | ID=MsaT024501.1;Parent=MsaG024501 |
| Chr4 | exon | 100187506 | 100187557 | 100187506 | ID=MsaT024501.1.exon1;Parent=MsaT024501.1 |
| Chr4 | CDS | 100187506 | 100187557 | 100187506 | ID=cds.MsaT024501.1;Parent=MsaT024501.1 |
| Chr4 | exon | 100188413 | 100188601 | 100188413 | ID=MsaT024501.1.exon2;Parent=MsaT024501.1 |
| Chr4 | CDS | 100188413 | 100188601 | 100188413 | ID=cds.MsaT024501.1;Parent=MsaT024501.1 |
| Chr4 | exon | 100192327 | 100192337 | 100192327 | ID=MsaT024501.1.exon3;Parent=MsaT024501.1 |
| Chr4 | CDS | 100192327 | 100192337 | 100192327 | ID=cds.MsaT024501.1;Parent=MsaT024501.1 |
| Gene Sequence |
| Protein sequence |
