AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG024523

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024523 MtrunA17_Chr8g0390841 85.872 453 39 3 1 430 71 521 0.0 781
MsaG024523 MtrunA17_Chr1g0208771 70.070 431 126 3 1 428 29 459 0.0 622
MsaG024523 MtrunA17_Chr1g0163071 49.477 287 128 8 1 274 18 300 1.85e-81 253
MsaG024523 MtrunA17_Chr7g0233561 37.500 280 136 9 3 262 52 312 3.01e-47 165
MsaG024523 MtrunA17_Chr7g0233541 36.559 279 144 10 1 262 18 280 9.43e-44 155
MsaG024523 MtrunA17_Chr3g0131891 36.301 292 149 9 3 280 53 321 6.93e-43 153
MsaG024523 MtrunA17_Chr7g0233531 36.620 284 116 9 3 262 20 263 2.26e-39 142
MsaG024523 MtrunA17_Chr7g0233521 36.131 274 135 9 1 262 18 263 3.67e-37 137
MsaG024523 MtrunA17_Chr1g0163181 27.689 437 259 16 1 425 37 428 1.38e-36 139
MsaG024523 MtrunA17_Chr1g0163201 26.233 446 219 16 1 425 35 391 7.85e-33 128
MsaG024523 MtrunA17_Chr3g0103181 30.325 277 138 9 1 261 42 279 1.09e-31 122
MsaG024523 MtrunA17_Chr3g0103181 39.583 96 56 2 332 426 185 279 2.80e-11 63.9
MsaG024523 MtrunA17_Chr3g0103191 30.453 243 121 9 1 228 42 251 6.56e-30 116
MsaG024523 MtrunA17_Chr3g0103171 28.986 276 149 8 1 261 22 265 1.64e-28 113
MsaG024523 MtrunA17_Chr3g0103171 39.583 144 75 6 287 426 130 265 4.15e-17 80.9
MsaG024523 MtrunA17_Chr3g0103201 28.405 257 122 7 27 261 4 220 2.33e-25 103
MsaG024523 MtrunA17_Chr1g0163251 31.104 299 167 13 1 288 37 307 5.85e-25 105
MsaG024523 MtrunA17_Chr7g0233571 31.937 191 107 4 1 184 42 216 3.67e-24 100
MsaG024523 MtrunA17_Chr3g0103151 39.655 116 63 1 1 109 42 157 1.57e-23 96.3
MsaG024523 MtrunA17_Chr4g0067431 30.258 271 154 12 19 270 66 320 3.23e-23 99.8
MsaG024523 MtrunA17_Chr1g0163151 27.536 276 132 7 161 425 2 220 3.03e-22 94.7
MsaG024523 MtrunA17_Chr1g0163111 28.777 278 133 9 161 425 2 227 8.76e-22 93.6
MsaG024523 MtrunA17_Chr5g0429931 27.820 266 134 9 3 262 29 242 5.52e-21 91.7
MsaG024523 MtrunA17_Chr1g0163261 28.782 271 152 8 6 265 46 286 6.24e-20 89.7
MsaG024523 MtrunA17_Chr3g0077451 26.568 271 128 7 3 265 30 237 2.99e-19 86.7
MsaG024523 MtrunA17_Chr1g0154041 21.495 535 301 21 3 425 49 576 4.68e-19 89.7
MsaG024523 MtrunA17_Chr4g0033961 33.333 177 106 6 107 272 224 399 1.43e-18 87.4
MsaG024523 MtrunA17_Chr4g0033961 31.061 132 85 1 3 128 40 171 9.12e-14 72.8
MsaG024523 MtrunA17_Chr5g0419201 35.294 136 76 3 3 129 50 182 1.96e-18 83.2
MsaG024523 MtrunA17_Chr1g0163221 26.592 267 161 10 19 275 62 303 1.35e-17 84.0
MsaG024523 MtrunA17_Chr3g0077411 24.632 272 141 8 3 261 296 516 4.02e-17 83.6
MsaG024523 MtrunA17_Chr3g0077431 26.054 261 122 7 13 265 1 198 5.40e-16 76.3
MsaG024523 MtrunA17_Chr3g0103161 40.952 105 52 4 325 425 24 122 9.83e-16 73.6
MsaG024523 MtrunA17_Chr1g0162441 37.391 115 65 4 160 268 285 398 1.19e-15 78.6
MsaG024523 MtrunA17_Chr1g0162441 43.939 66 37 0 3 68 41 106 9.65e-12 66.6
MsaG024523 MtrunA17_Chr1g0163231 26.165 279 145 11 1 260 41 277 4.30e-15 75.5
MsaG024523 MtrunA17_Chr1g0154161 24.286 280 168 12 3 268 64 313 1.77e-14 73.9
MsaG024523 MtrunA17_Chr1g0154191 24.756 307 184 12 3 266 56 358 2.69e-14 73.9
MsaG024523 MtrunA17_Chr1g0154111 24.919 309 181 13 3 266 60 362 2.54e-13 72.0
MsaG024523 MtrunA17_Chr1g0154131 24.231 260 166 8 3 249 216 457 3.86e-13 70.9
MsaG024523 MtrunA17_Chr1g0163091 36.220 127 68 6 157 275 10 131 1.17e-12 65.9
MsaG024523 MtrunA17_Chr1g0154171 24.585 301 181 13 3 266 56 347 1.44e-11 65.5
MsaG024523 MtrunA17_Chr1g0154151 22.185 302 175 11 3 266 38 317 3.05e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024523 AT3G18990.1 34.323 303 156 12 3 265 40 339 5.91e-40 146
MsaG024523 AT3G18990.2 33.993 303 157 12 3 265 40 339 5.87e-39 144
MsaG024523 AT1G49480.3 38.393 112 61 4 160 265 115 224 5.38e-18 83.2
MsaG024523 AT1G49480.1 38.393 112 61 4 160 265 115 224 5.38e-18 83.2
MsaG024523 AT1G49480.2 38.393 112 61 4 160 265 115 224 5.38e-18 83.2
MsaG024523 AT4G01580.1 44.928 69 38 0 3 71 64 132 3.00e-13 68.6
MsaG024523 AT3G18960.2 43.478 69 39 0 3 71 64 132 3.33e-13 68.6
MsaG024523 AT3G18960.3 43.478 69 39 0 3 71 27 95 5.29e-13 67.4
MsaG024523 AT3G18960.1 43.478 69 39 0 3 71 64 132 5.96e-13 68.2
MsaG024523 AT4G33280.1 26.087 276 175 10 3 262 57 319 3.92e-12 67.8
MsaG024523 AT1G49475.1 37.179 78 49 0 3 80 67 144 7.11e-12 64.7
MsaG024523 AT4G31640.1 25.794 252 149 8 186 430 25 245 2.34e-11 65.5

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAACTTGTCCAAGTTAA+AGG 0.118978 4:+100402352 MsaT024523.1:CDS
GAACAAACTTGTTGAAAATT+TGG 0.135205 4:+100402207 MsaT024523.1:CDS
CTTGATCATGCTTCACATTT+AGG 0.162093 4:-100402505 None:intergenic
TGTCATGACAACAGTGAAAA+AGG 0.224513 4:-100402597 None:intergenic
CCTTTAACTTGGACAAGTTT+AGG 0.279816 4:-100402352 None:intergenic
TTAATGGAGATGGAATTAAT+TGG 0.309089 4:+100403114 MsaT024523.1:CDS
GTTTGTAGAGGAAAATAAAT+TGG 0.310756 4:+100403924 MsaT024523.1:CDS
TACTTTAGTTCAAAATCTAC+TGG 0.361077 4:-100402779 None:intergenic
CTCAATTTCTTATGTGGTTG+AGG 0.369014 4:-100402280 None:intergenic
GGAAGTACATTTGACCAAAA+AGG 0.369637 4:+100402009 MsaT024523.1:CDS
CTTCACATTTAGGCATTTAG+TGG 0.383770 4:-100402495 None:intergenic
CAGAGTGGATGTGAATTGAT+TGG 0.396589 4:-100403511 None:intergenic
TAATGGAGATGGAATTAATT+GGG 0.397926 4:+100403115 MsaT024523.1:CDS
AAAACTATCATGTCACATAA+AGG 0.413575 4:+100403170 MsaT024523.1:CDS
CATATTTAACAAGAGTACTT+TGG 0.415158 4:+100402144 MsaT024523.1:CDS
ACTCAACTTGGTTGACGGTT+CGG 0.425773 4:-100403887 None:intergenic
TCTCATTATCCATGACTTGA+AGG 0.426650 4:-100402829 None:intergenic
CCAATTCTCAATTTCTTATG+TGG 0.430787 4:-100402286 None:intergenic
CTTGTGACACAAGTACTTCT+CGG 0.432076 4:-100403803 None:intergenic
AAACCTGTGGCATGTAAGAT+TGG 0.435151 4:+100403852 MsaT024523.1:CDS
ATATTTGGATTCACAAGGGT+TGG 0.435488 4:+100402038 MsaT024523.1:CDS
CCACATAAGAAATTGAGAAT+TGG 0.439783 4:+100402286 MsaT024523.1:CDS
AATTTATTATACCTTTAACT+TGG 0.446187 4:-100402363 None:intergenic
CCTGTACTCAACTTGGTTGA+CGG 0.457593 4:-100403892 None:intergenic
GATCTAGTCTTCTTGCATGT+AGG 0.464753 4:-100402238 None:intergenic
TTTGATAATAATTTGAAAGT+GGG 0.464811 4:+100402911 MsaT024523.1:CDS
TAGAATTACCTTGCGGTGGA+AGG 0.469307 4:-100403015 None:intergenic
CTTGGAATGTTAAACATTGT+GGG 0.475665 4:+100402855 MsaT024523.1:CDS
TTGGCATACCACAGGAAAGA+AGG 0.492460 4:+100403425 MsaT024523.1:intron
TCATGGATAATGAGAGAACT+TGG 0.492868 4:+100402837 MsaT024523.1:CDS
ATTTGATAATAATTTGAAAG+TGG 0.495131 4:+100402910 MsaT024523.1:CDS
CTTGAATCAGTTAATGGAGA+TGG 0.499186 4:+100403104 MsaT024523.1:intron
TCTGGAGTCTTGAGAAACAT+TGG 0.510455 4:-100401974 None:intergenic
ACTTGGAATGTTAAACATTG+TGG 0.512866 4:+100402854 MsaT024523.1:CDS
TGTGGGAGACATATTAGTAC+TGG 0.514312 4:+100402872 MsaT024523.1:CDS
AACTTGGTTGACGGTTCGGA+TGG 0.514973 4:-100403883 None:intergenic
CGTTATCACCCTTCAAGTCA+TGG 0.529744 4:+100402820 MsaT024523.1:CDS
ACTCCAACCTGTACTCAACT+TGG 0.532809 4:-100403899 None:intergenic
TGTAGAGGAAAATAAATTGG+AGG 0.533957 4:+100403927 MsaT024523.1:CDS
GAGGAAAATAAATTGGAGGA+TGG 0.537388 4:+100403931 MsaT024523.1:CDS
TCGCCAATCTTACATGCCAC+AGG 0.542280 4:-100403855 None:intergenic
CTCATTATCCATGACTTGAA+GGG 0.556492 4:-100402828 None:intergenic
AGAATTACCTTGCGGTGGAA+GGG 0.558988 4:-100403014 None:intergenic
TCATGCGGATGTTTACAAAC+CGG 0.561557 4:+100403569 MsaT024523.1:CDS
GAAACATCAGCATGTGTCCA+TGG 0.562484 4:-100402090 None:intergenic
CCGTCAACCAAGTTGAGTAC+AGG 0.579549 4:+100403892 MsaT024523.1:CDS
AGGTTGGAGTAAGTTTGTAG+AGG 0.581140 4:+100403912 MsaT024523.1:CDS
TGGTGATATTTGGATTCACA+AGG 0.584168 4:+100402033 MsaT024523.1:CDS
TTCACTTTGATAGTGAAATG+TGG 0.584688 4:-100403540 None:intergenic
CAACTAGAATTACCTTGCGG+TGG 0.590450 4:-100403019 None:intergenic
ACTTTAGTTCAAAATCTACT+GGG 0.593567 4:-100402778 None:intergenic
ATTGGCGCAAGTGAAGATGT+TGG 0.596112 4:+100402304 MsaT024523.1:CDS
TTCCTATGCTAATAAATACA+TGG 0.597714 4:+100402622 MsaT024523.1:CDS
GGAGACATATTAGTACTGGA+TGG 0.606406 4:+100402876 MsaT024523.1:CDS
CACCATGTATTTATTAGCAT+AGG 0.611640 4:-100402624 None:intergenic
CAACCAAGTTGAGTACAGGT+TGG 0.611957 4:+100403896 MsaT024523.1:CDS
AAACATTCCCTTCCACCGCA+AGG 0.615531 4:+100403007 MsaT024523.1:CDS
ACACATTATTCTATAGACCA+TGG 0.617727 4:+100402073 MsaT024523.1:CDS
TATGTAAACCTCAAAATGAG+AGG 0.618304 4:-100402125 None:intergenic
CAAAGTGAAATCAAGTCATG+CGG 0.640337 4:+100403554 MsaT024523.1:CDS
AAGTATTTGAAGGAAAAGCA+AGG 0.649480 4:+100402797 MsaT024523.1:CDS
GGTGATATTTGGATTCACAA+GGG 0.673550 4:+100402034 MsaT024523.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 100401977 100404029 100401977 ID=MsaG024523
Chr4 mRNA 100401977 100404029 100401977 ID=MsaT024523.1;Parent=MsaG024523
Chr4 exon 100401977 100402373 100401977 ID=MsaT024523.1.exon1;Parent=MsaT024523.1
Chr4 CDS 100401977 100402373 100401977 ID=cds.MsaT024523.1;Parent=MsaT024523.1
Chr4 exon 100402480 100402643 100402480 ID=MsaT024523.1.exon2;Parent=MsaT024523.1
Chr4 CDS 100402480 100402643 100402480 ID=cds.MsaT024523.1;Parent=MsaT024523.1
Chr4 exon 100402773 100403028 100402773 ID=MsaT024523.1.exon3;Parent=MsaT024523.1
Chr4 CDS 100402773 100403028 100402773 ID=cds.MsaT024523.1;Parent=MsaT024523.1
Chr4 exon 100403114 100403191 100403114 ID=MsaT024523.1.exon4;Parent=MsaT024523.1
Chr4 CDS 100403114 100403191 100403114 ID=cds.MsaT024523.1;Parent=MsaT024523.1
Chr4 exon 100403439 100403590 100403439 ID=MsaT024523.1.exon5;Parent=MsaT024523.1
Chr4 CDS 100403439 100403590 100403439 ID=cds.MsaT024523.1;Parent=MsaT024523.1
Chr4 exon 100403784 100404029 100403784 ID=MsaT024523.1.exon6;Parent=MsaT024523.1
Chr4 CDS 100403784 100404029 100403784 ID=cds.MsaT024523.1;Parent=MsaT024523.1
Gene Sequence

>MsaT024523.1

ATGTTTCTCAAGACTCCAGATGACAAAAAATGGGAAGTACATTTGACCAAAAAGGATGGTGATATTTGGATTCACAAGGGTTGGAATGAATTTGCTACACATTATTCTATAGACCATGGACACATGCTGATGTTTCAATATGAAAAAACCTCTCATTTTGAGGTTTACATATTTAACAAGAGTACTTTGGAAATTGAATATCACGTTGATAGTAATAATCAACATGAACAGAACAAACTTGTTGAAAATTTGGATGAAAAACCTACATGCAAGAAGACTAGATCAAAATCACAAATTTCAAGTCCTCAACCACATAAGAAATTGAGAATTGGCGCAAGTGAAGATGTTGGAACAAGCTCCAAATTGAAAAAAATTCCTAAACTTGTCCAAGTTAAAGAGGAGATTGAAGATACCACTAAATGCCTAAATGTGAAGCATGATCAAGAGCCGAATAATTCATCTTCAAAAATTGCTGAAGCTTTAAACAAAGCAAAAAACTACAAAACTAACAATCCCTTTTTCACTGTTGTCATGACATATTCCTATGCTAATAAATACATGTACATCCCAGTAGATTTTGAACTAAAGTATTTGAAGGAAAAGCAAGGCGTTATCACCCTTCAAGTCATGGATAATGAGAGAACTTGGAATGTTAAACATTGTGGGAGACATATTAGTACTGGATGGAAAAATTTTGCATTTGATAATAATTTGAAAGTGGGAGATGTTTGTCTTTTTGAGATGATCAAAAGCAATTCTTATGCTTTCAAAGTACTAATTTTTCGACTAGGCGAAAAACATTCCCTTCCACCGCAAGTTAATGGAGATGGAATTAATTGGGTTGAAACAGCAAGAATCCCTAGAGTTGAACATAAAACTATCATGTCACATAAAGGAAAGAAGGCAACTCAAAATTCACTGAATGCTAGTTCACGCTCTTTCAAAAGTTTTGAAGTTATGAAAGAATCCAATCAATTCACATCCACTCTGAAAAATCCACATTTCACTATCAAAGTGAAATCAAGTCATGCGGATGTTTACAAACCGCGTATACACAACTCGTTCTCCGAGAAGTACTTGTGTCACAAGAAGATTGTAACGTTACAGTTTAATAAAAACCTGTGGCATGTAAGATTGGCGAGCTGTCCATCCGAACCGTCAACCAAGTTGAGTACAGGTTGGAGTAAGTTTGTAGAGGAAAATAAATTGGAGGATGGAGATTCATGTGTTTTTGAGCTCGTCAATAAGGAAGATCTTGTGTTTGATGCTCATATTTTCAGAGGCTGCAATTAG
Protein sequence

>MsaT024523.1

MFLKTPDDKKWEVHLTKKDGDIWIHKGWNEFATHYSIDHGHMLMFQYEKTSHFEVYIFNKSTLEIEYHVDSNNQHEQNKLVENLDEKPTCKKTRSKSQISSPQPHKKLRIGASEDVGTSSKLKKIPKLVQVKEEIEDTTKCLNVKHDQEPNNSSSKIAEALNKAKNYKTNNPFFTVVMTYSYANKYMYIPVDFELKYLKEKQGVITLQVMDNERTWNVKHCGRHISTGWKNFAFDNNLKVGDVCLFEMIKSNSYAFKVLIFRLGEKHSLPPQVNGDGINWVETARIPRVEHKTIMSHKGKKATQNSLNASSRSFKSFEVMKESNQFTSTLKNPHFTIKVKSSHADVYKPRIHNSFSEKYLCHKKIVTLQFNKNLWHVRLASCPSEPSTKLSTGWSKFVEENKLEDGDSCVFELVNKEDLVFDAHIFRGCN*