AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG024532

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024532 MtrunA17_Chr8g0390971 97.356 416 11 0 1 416 1 416 0.0 847
MsaG024532 MtrunA17_Chr1g0209861 42.222 450 187 17 11 412 9 433 4.24e-83 261
MsaG024532 MtrunA17_Chr5g0431351 40.288 139 55 4 274 400 94 216 4.60e-18 82.4
MsaG024532 MtrunA17_Chr4g0071811 47.170 106 40 3 297 400 293 384 6.97e-17 82.0
MsaG024532 MtrunA17_Chr7g0226451 38.235 170 55 8 276 400 187 351 2.24e-16 80.1
MsaG024532 MtrunA17_Chr4g0072871 53.623 69 30 1 333 401 275 341 1.03e-12 69.3
MsaG024532 MtrunA17_Chr3g0136701 70.455 44 13 0 358 401 232 275 1.24e-11 65.1
MsaG024532 MtrunA17_Chr7g0220721 70.455 44 13 0 358 401 327 370 1.73e-11 65.5
MsaG024532 MtrunA17_Chr1g0150471 68.182 44 14 0 358 401 255 298 2.02e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024532 AT1G25440.1 42.466 438 164 16 11 400 6 403 9.92e-84 262
MsaG024532 AT1G68520.1 41.981 424 186 14 11 402 6 401 1.36e-82 259
MsaG024532 AT1G73870.1 38.303 436 188 17 1 402 1 389 4.34e-67 219
MsaG024532 AT1G49130.1 44.755 143 58 5 16 151 22 150 3.64e-27 110
MsaG024532 AT1G49130.1 48.598 107 34 4 295 401 236 321 5.40e-18 84.7
MsaG024532 AT1G49130.2 44.755 143 58 5 16 151 15 143 4.30e-27 110
MsaG024532 AT1G49130.2 48.598 107 34 4 295 401 229 314 5.74e-18 84.7
MsaG024532 AT5G57180.2 43.704 135 55 3 269 400 309 425 3.02e-21 95.5
MsaG024532 AT1G07050.1 38.286 175 80 5 235 400 38 193 3.81e-20 88.2
MsaG024532 AT4G25990.1 47.458 118 42 4 285 400 284 383 1.32e-19 90.5
MsaG024532 AT5G57180.1 41.860 129 54 3 269 394 309 419 1.30e-17 84.7
MsaG024532 AT5G14370.1 42.593 108 59 2 294 400 231 336 2.06e-16 80.5
MsaG024532 AT4G25990.2 42.105 133 42 5 285 400 284 398 2.96e-16 80.5
MsaG024532 AT4G25990.3 45.536 112 41 4 285 394 284 377 5.07e-16 79.7
MsaG024532 AT1G07050.2 36.095 169 80 5 235 394 38 187 7.08e-16 76.3
MsaG024532 AT5G57660.1 70.455 44 13 0 358 401 285 328 1.94e-11 65.5
MsaG024532 AT5G24930.1 70.455 44 13 0 358 401 295 338 2.34e-11 65.5
MsaG024532 AT2G24790.1 70.455 44 13 0 358 401 229 272 3.77e-11 63.9
MsaG024532 AT2G24790.1 53.030 66 27 1 16 77 2 67 5.30e-11 63.5
MsaG024532 AT2G24790.2 53.030 66 27 1 16 77 2 67 3.78e-11 63.2

Find 63 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAAACGTCCTTTCATTCT+TGG 0.215353 4:-100534858 None:intergenic
TGATTCATGGCAAAACTTAT+TGG 0.234314 4:+100534417 MsaT024532.1:CDS
TTGTCATGGTTGTGACAATT+TGG 0.309873 4:+100533562 MsaT024532.1:CDS
ATCACATGCTCTTGCTGTCT+TGG 0.344245 4:-100533486 None:intergenic
ATGGAGAAGTAAGAAGCTTA+AGG 0.355490 4:+100534703 MsaT024532.1:CDS
AAATGAAATGTTAAAGGTTA+AGG 0.373350 4:+100534111 MsaT024532.1:CDS
TCTTCATCACCTCCGAGTTC+AGG 0.373850 4:-100533794 None:intergenic
CAGGGTTCAAGTGGTGTAGA+TGG 0.388893 4:+100534684 MsaT024532.1:intron
TCATGTCAGTGGTAATTAGT+TGG 0.390665 4:-100534921 None:intergenic
CTTCCACTTGTTCCTGAACT+CGG 0.406110 4:+100533782 MsaT024532.1:CDS
TGGAGAAGTAAGAAGCTTAA+GGG 0.411931 4:+100534704 MsaT024532.1:CDS
TCAATTGTGATGATGATTCA+TGG 0.426751 4:+100534404 MsaT024532.1:CDS
GATGCAGATGCAGGTGCCTT+GGG 0.430470 4:+100533461 MsaT024532.1:CDS
AAAGGGCTGACATCGAAAAC+TGG 0.442133 4:-100533881 None:intergenic
CCTTAGCAGACGCGGTTTGA+AGG 0.445349 4:-100533628 None:intergenic
AGAAGATAAGATATGAAGTA+AGG 0.448594 4:+100534814 MsaT024532.1:CDS
CCTTCAAACCGCGTCTGCTA+AGG 0.473927 4:+100533628 MsaT024532.1:CDS
GTTGAAAGTTTACTTGGTGT+TGG 0.483990 4:+100534013 MsaT024532.1:CDS
TTAATGGGAAGTGGTGGAGA+TGG 0.488245 4:+100534732 MsaT024532.1:CDS
GAGATTCGAGAGAATTCATC+AGG 0.489115 4:+100534043 MsaT024532.1:CDS
TCTTGCTGTCTTGGCTCCCA+AGG 0.495634 4:-100533477 None:intergenic
TTATAAACAAGAGAATGAAA+TGG 0.496628 4:+100534078 MsaT024532.1:CDS
TCATCACAATTGAACTTAGG+AGG 0.497589 4:-100534394 None:intergenic
CCTTTCAGTAATAGTAGTGT+TGG 0.498513 4:+100534247 MsaT024532.1:CDS
TTGAAAGTTTACTTGGTGTT+GGG 0.498767 4:+100534014 MsaT024532.1:CDS
TGATGCAGATGCAGGTGCCT+TGG 0.503435 4:+100533460 MsaT024532.1:CDS
GCTAATGTTGAAAGTTTACT+TGG 0.513191 4:+100534007 MsaT024532.1:CDS
GGAGGGTTTGCAGTAGTCCA+TGG 0.516903 4:-100534376 None:intergenic
ATGGGAAGTGGTGGAGATGG+AGG 0.526179 4:+100534735 MsaT024532.1:CDS
ATGAAGAGGTTATTACTGAA+TGG 0.526407 4:+100534332 MsaT024532.1:CDS
TACAAGTCTCTAAATCGAAA+GGG 0.527173 4:-100533898 None:intergenic
GATGATGCTTTCCTTTGTCA+TGG 0.527738 4:+100533548 MsaT024532.1:CDS
GGAGAAGTAAGAAGCTTAAG+GGG 0.532873 4:+100534705 MsaT024532.1:CDS
ATTAGCAAGTAGACACGAAA+GGG 0.537189 4:+100533601 MsaT024532.1:CDS
TCATCATCACAATTGAACTT+AGG 0.539550 4:-100534397 None:intergenic
GTACAAGTCTCTAAATCGAA+AGG 0.540349 4:-100533899 None:intergenic
CATCACAATTGAACTTAGGA+GGG 0.544271 4:-100534393 None:intergenic
GAAGAGAAGCAAGAGTATCA+AGG 0.553110 4:+100534757 MsaT024532.1:CDS
TTTGAGACTAAACTATGAAG+AGG 0.554254 4:+100534318 MsaT024532.1:CDS
GAAAAGAGACCAAGAATGAA+AGG 0.557120 4:+100534849 MsaT024532.1:CDS
GGCAAGGTTCTCCTTCTCCA+TGG 0.557589 4:+100534359 MsaT024532.1:CDS
ATTACTGAATGGAGCAGGCA+AGG 0.558438 4:+100534343 MsaT024532.1:CDS
AGGTTATTACTGAATGGAGC+AGG 0.563538 4:+100534338 MsaT024532.1:CDS
TATTGATTTCAGGGTTCAAG+TGG 0.571240 4:+100534675 MsaT024532.1:intron
ATGAAAGGTGATGCAGATGC+AGG 0.575851 4:+100533452 MsaT024532.1:CDS
ACAACAACAGCAACGTCTCA+AGG 0.580637 4:+100533733 MsaT024532.1:CDS
GGGCAATTAATGGGAAGTGG+TGG 0.598711 4:+100534726 MsaT024532.1:CDS
CCTCCGAGTTCAGGAACAAG+TGG 0.605265 4:-100533785 None:intergenic
TATTAGCAAGTAGACACGAA+AGG 0.608632 4:+100533600 MsaT024532.1:CDS
TTTGCAGTAGTCCATGGAGA+AGG 0.610228 4:-100534370 None:intergenic
CCAACACTACTATTACTGAA+AGG 0.612166 4:-100534247 None:intergenic
TAACAACAACAGCACAAGCA+TGG 0.618954 4:+100533651 MsaT024532.1:CDS
TGATATTGATGAGGCTGATG+AGG 0.627860 4:+100533838 MsaT024532.1:CDS
ACTACTTGTTGATATTGATG+AGG 0.630146 4:+100533829 MsaT024532.1:CDS
AATTGTCACAACCATGACAA+AGG 0.630486 4:-100533559 None:intergenic
TGTTGTTACCTTAGCAGACG+CGG 0.639555 4:-100533636 None:intergenic
CATGGCACAGTGGATTCACA+AGG 0.649315 4:+100533669 MsaT024532.1:CDS
TCTATGATTACAGACATGAA+AGG 0.650216 4:+100533437 None:intergenic
CTCGGAGGTGATGAAGAACA+AGG 0.666390 4:+100533800 MsaT024532.1:CDS
CCACTTGTTCCTGAACTCGG+AGG 0.668175 4:+100533785 MsaT024532.1:CDS
GTTTAAGAAGAAGAGCACGT+TGG 0.700628 4:+100533513 MsaT024532.1:CDS
GAGAAGTAAGAAGCTTAAGG+GGG 0.726133 4:+100534706 MsaT024532.1:CDS
ACAGCACAAGCATGGCACAG+TGG 0.751381 4:+100533659 MsaT024532.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 100533440 100534938 100533440 ID=MsaG024532
Chr4 mRNA 100533440 100534938 100533440 ID=MsaT024532.1;Parent=MsaG024532
Chr4 exon 100533440 100534438 100533440 ID=MsaT024532.1.exon1;Parent=MsaT024532.1
Chr4 CDS 100533440 100534438 100533440 ID=cds.MsaT024532.1;Parent=MsaT024532.1
Chr4 exon 100534687 100534938 100534687 ID=MsaT024532.1.exon2;Parent=MsaT024532.1
Chr4 CDS 100534687 100534938 100534687 ID=cds.MsaT024532.1;Parent=MsaT024532.1
Gene Sequence

>MsaT024532.1

ATGATTACAGACATGAAAGGTGATGCAGATGCAGGTGCCTTGGGAGCCAAGACAGCAAGAGCATGTGATAGCTGTTTAAGAAGAAGAGCACGTTGGTTTTGTGCTGCAGATGATGCTTTCCTTTGTCATGGTTGTGACAATTTGGTTCACTCTGCAAATCTATTAGCAAGTAGACACGAAAGGGTTCGCCTTCAAACCGCGTCTGCTAAGGTAACAACAACAGCACAAGCATGGCACAGTGGATTCACAAGGAAAGCAAGAACGCCCCGTCACAACAAAAATTCGTCGATTCAACAACAACAGCAACGTCTCAAGGAAAAAGTTCTTTTCAATACTAGTTTTCTTCCACTTGTTCCTGAACTCGGAGGTGATGAAGAACAAGGACAAGAACTACTTGTTGATATTGATGAGGCTGATGAGGAGCAGTTGCTGTGTCGTGTGCCAGTTTTCGATGTCAGCCCTTTCGATTTAGAGACTTGTACTGTGAAAAATGATGCTGTTGATTTTGAGGAAATGTGTGACTTGGATAGTTTCTGTGAGTTTGATGTTGATCTTGCTGAATTTGCAGCTAATGTTGAAAGTTTACTTGGTGTTGGGAGTAGTGAGATTCGAGAGAATTCATCAGGACAAGTGTTTGATTATAAACAAGAGAATGAAATGGATGCATCCAAAAATGAAATGTTAAAGGTTAAGGATGAAGAACTTGATGATTTAGAATCAGTTTTTGACATGACAAGTGATGAGGTTTTTCATTGGAATATTGATCATAATGATGTATCACTAGCACAACAAGAGAAAGAGTTTATGCCTTTCAGTAATAGTAGTGTTGGTTACAGTGAGAGTGTTATTACTAAAGAAGAAACAAAGAGAGAGAGATTTTTGAGACTAAACTATGAAGAGGTTATTACTGAATGGAGCAGGCAAGGTTCTCCTTCTCCATGGACTACTGCAAACCCTCCTAAGTTCAATTGTGATGATGATTCATGGCAAAACTTATTGGGTTCAAGTGGTGTAGATGGAGAAGTAAGAAGCTTAAGGGGGCAATTAATGGGAAGTGGTGGAGATGGAGGAAGAGAAGCAAGAGTATCAAGGTACAGAGAGAAAAGAAGAACACGTTTATTTGCAAAGAAGATAAGATATGAAGTAAGGAAATTGAATGCAGAAAAGAGACCAAGAATGAAAGGACGTTTTGTTAAGAGGACAGCATGTTTTGCTGGAGGAGCTACTTCTTTTCCAACTAATTACCACTGA
Protein sequence

>MsaT024532.1

MITDMKGDADAGALGAKTARACDSCLRRRARWFCAADDAFLCHGCDNLVHSANLLASRHERVRLQTASAKVTTTAQAWHSGFTRKARTPRHNKNSSIQQQQQRLKEKVLFNTSFLPLVPELGGDEEQGQELLVDIDEADEEQLLCRVPVFDVSPFDLETCTVKNDAVDFEEMCDLDSFCEFDVDLAEFAANVESLLGVGSSEIRENSSGQVFDYKQENEMDASKNEMLKVKDEELDDLESVFDMTSDEVFHWNIDHNDVSLAQQEKEFMPFSNSSVGYSESVITKEETKRERFLRLNYEEVITEWSRQGSPSPWTTANPPKFNCDDDSWQNLLGSSGVDGEVRSLRGQLMGSGGDGGREARVSRYREKRRTRLFAKKIRYEVRKLNAEKRPRMKGRFVKRTACFAGGATSFPTNYH*