AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG024592

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024592 MtrunA17_Chr8g0391911 80.545 257 36 3 1 250 1 250 8.38e-130 382
MsaG024592 MtrunA17_Chr6g0456631 88.889 171 18 1 1 170 1 171 6.52e-105 315
MsaG024592 MtrunA17_Chr4g0009851 41.463 246 118 3 9 254 11 230 4.07e-58 198
MsaG024592 MtrunA17_Chr4g0009901 42.353 255 120 4 1 254 1 229 2.11e-56 192
MsaG024592 MtrunA17_Chr1g0161431 39.773 264 133 6 8 253 10 265 2.00e-50 185
MsaG024592 MtrunA17_Chr3g0123131 38.672 256 147 5 8 257 12 263 2.12e-48 178
MsaG024592 MtrunA17_Chr8g0376881 38.492 252 149 4 9 257 25 273 1.64e-45 169
MsaG024592 MtrunA17_Chr2g0294911 39.592 245 144 3 7 250 27 268 2.18e-45 170
MsaG024592 MtrunA17_Chr4g0067981 41.200 250 135 8 7 250 29 272 2.25e-44 168
MsaG024592 MtrunA17_Chr3g0137101 35.836 293 175 6 6 288 5 294 1.35e-43 166
MsaG024592 MtrunA17_Chr6g0471041 39.662 237 113 6 14 248 19 227 2.19e-42 158
MsaG024592 MtrunA17_Chr1g0149851 33.533 334 190 7 6 309 2 333 1.14e-41 159
MsaG024592 MtrunA17_Chr8g0383961 53.333 135 61 2 4 137 9 142 2.05e-40 148
MsaG024592 MtrunA17_Chr1g0149841 38.000 250 145 5 7 250 3 248 2.67e-40 154
MsaG024592 MtrunA17_Chr7g0275891 34.520 281 160 6 6 269 2 275 2.22e-39 148
MsaG024592 MtrunA17_Chr2g0304841 36.434 258 147 6 7 250 16 270 3.83e-36 144
MsaG024592 MtrunA17_Chr4g0074401 35.409 257 146 6 5 250 13 260 8.31e-36 142
MsaG024592 MtrunA17_Chr4g0009981 37.438 203 101 3 52 254 1 177 4.86e-35 133
MsaG024592 MtrunA17_Chr4g0074411 33.716 261 134 6 9 250 18 258 1.52e-34 139
MsaG024592 MtrunA17_Chr7g0275401 31.818 286 162 7 5 271 1 272 7.77e-34 130
MsaG024592 MtrunA17_Chr1g0149831 37.313 201 90 6 67 250 1 182 4.65e-28 112
MsaG024592 MtrunA17_Chr4g0074421 35.079 191 118 4 58 247 2 187 3.67e-26 106
MsaG024592 MtrunA17_Chr4g0011071 34.810 158 79 2 54 211 35 168 1.00e-25 104
MsaG024592 MtrunA17_Chr3g0134461 34.016 244 107 7 1 242 1 192 1.03e-25 105
MsaG024592 MtrunA17_Chr4g0010001 48.750 80 41 0 9 88 10 89 3.84e-22 91.3
MsaG024592 MtrunA17_Chr5g0423031 50.000 80 40 0 9 88 10 89 8.98e-20 87.4
MsaG024592 MtrunA17_Chr3g0134481 36.220 127 79 2 5 130 4 129 1.46e-19 87.0
MsaG024592 MtrunA17_Chr8g0350761 25.795 283 156 8 11 257 19 283 1.32e-18 89.4
MsaG024592 MtrunA17_Chr4g0009991 36.620 142 64 3 101 242 1 116 2.00e-16 75.9
MsaG024592 MtrunA17_Chr6g0473731 30.899 178 80 5 89 254 10 156 1.19e-14 76.6
MsaG024592 MtrunA17_Chr7g0255711 53.030 66 29 1 187 252 87 150 2.52e-13 71.2
MsaG024592 MtrunA17_Chr4g0031961 51.351 74 29 2 179 252 124 190 1.69e-12 68.9
MsaG024592 MtrunA17_Chr5g0422621 37.037 108 64 2 143 250 153 256 4.01e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024592 AT1G67710.1 38.462 260 137 5 10 256 8 257 4.01e-47 174
MsaG024592 AT2G01760.1 38.235 272 141 8 5 258 3 265 2.14e-45 166
MsaG024592 AT3G16857.1 36.940 268 137 7 9 250 33 294 2.96e-41 160
MsaG024592 AT3G16857.2 36.940 268 137 7 9 250 33 294 4.13e-41 159
MsaG024592 AT2G01760.2 34.667 300 141 9 5 258 3 293 2.29e-39 150
MsaG024592 AT2G25180.1 36.948 249 145 6 7 250 11 252 6.36e-39 152
MsaG024592 AT4G31920.1 35.249 261 121 7 7 250 11 240 6.81e-36 142
MsaG024592 AT5G58080.1 32.754 345 189 9 7 323 12 341 1.79e-35 142
MsaG024592 AT1G67710.2 39.604 202 100 4 67 256 1 192 2.80e-35 139
MsaG024592 AT3G16857.3 37.555 229 111 7 48 250 2 224 9.72e-33 134
MsaG024592 AT5G49240.1 33.466 251 156 7 5 249 34 279 7.21e-31 122
MsaG024592 AT2G01760.3 38.756 209 102 7 67 258 1 200 7.23e-31 123
MsaG024592 AT1G49190.1 31.698 265 162 7 4 256 25 282 2.20e-29 121
MsaG024592 AT3G62670.1 31.741 293 180 8 14 301 42 319 2.61e-29 121
MsaG024592 AT1G67710.3 37.766 188 94 4 83 256 5 183 1.24e-27 116
MsaG024592 AT5G07210.2 24.096 332 204 9 13 312 16 331 1.54e-17 87.0
MsaG024592 AT1G49190.2 33.588 131 85 2 4 133 25 154 5.65e-17 85.1
MsaG024592 AT1G68210.1 29.412 272 153 9 8 245 8 274 3.99e-16 82.8
MsaG024592 AT5G07210.1 23.496 349 207 11 13 321 16 344 3.11e-15 79.7
MsaG024592 AT5G05090.1 49.383 81 31 2 170 250 68 138 1.08e-13 72.0
MsaG024592 AT3G10760.1 53.030 66 29 1 187 252 101 164 1.08e-13 73.2
MsaG024592 AT5G59570.2 53.030 66 29 1 187 252 137 200 1.48e-13 72.4
MsaG024592 AT5G59570.1 53.030 66 29 1 187 252 137 200 1.48e-13 72.4
MsaG024592 AT2G40970.1 42.718 103 46 3 176 275 98 190 2.83e-13 70.5
MsaG024592 AT3G46640.2 53.030 66 29 1 187 252 140 203 3.57e-13 71.6
MsaG024592 AT3G46640.1 53.030 66 29 1 187 252 140 203 3.57e-13 71.6
MsaG024592 AT3G46640.3 53.030 66 29 1 187 252 140 203 4.87e-13 71.2

Find 189 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCATCTCTTGCCCTTATT+TGG 0.189913 4:-101245833 None:intergenic
TTCTTTGTCCGGGGTGGTTC+GGG 0.278029 4:-101245906 None:intergenic
CTTCTTTGTCCGGGGTGGTT+CGG 0.314627 4:-101245907 None:intergenic
GGATTGAGGGTAGCCAAATA+AGG 0.317246 4:+101245820 MsaT024592.1:CDS
GGTGACTGGTCCTCAGGCTC+AGG 0.325098 4:+101247944 MsaT024592.1:CDS
AACGATGTACCATCGGAAAA+AGG 0.331149 4:+101245870 MsaT024592.1:CDS
AATAATACTGTAACAGATTT+CGG 0.331250 4:+101247315 MsaT024592.1:CDS
ATAATGAAGGCAATCGAAAA+TGG 0.339395 4:+101245681 MsaT024592.1:CDS
TCTTCTTTGTCTTCTTTGTC+CGG 0.340590 4:-101245917 None:intergenic
CAATTAGTATATCTTGTGTT+AGG 0.345112 4:-101248044 None:intergenic
AAGGGTGGTGGTTGGTCCTC+AGG 0.351815 4:+101247422 MsaT024592.1:CDS
AAGGGTGGTGGCTGGTCCTC+AGG 0.380509 4:+101247818 MsaT024592.1:CDS
AAAGGTGGTGGCTGCTCGTC+AGG 0.385388 4:+101247500 MsaT024592.1:CDS
CTTCTTTGTCTTCTTTGTCC+GGG 0.397717 4:-101245916 None:intergenic
GCAGGTAGCTAGTCATCTTC+AGG 0.404754 4:+101246345 MsaT024592.1:CDS
CTTGAATTTGATAGCACAAA+AGG 0.421977 4:+101245427 MsaT024592.1:CDS
CTCAGCCTCAAGAGCTAGGA+AGG 0.422711 4:+101247565 MsaT024592.1:CDS
CATGATCATGCAGTTGGTCT+TGG 0.425207 4:+101247153 MsaT024592.1:CDS
GAGCTGGGAAGGGTGGTGGC+TGG 0.425258 4:+101247378 MsaT024592.1:CDS
AAGGATGGCGGCTGGTCCTC+AGG 0.425842 4:+101247464 MsaT024592.1:CDS
GCAAGTGGAGGTTGGTCCTC+AGG 0.426953 4:+101247350 MsaT024592.1:CDS
ACAAGAACTTTAACATTGGC+TGG 0.429953 4:-101245209 None:intergenic
AAGGGTGGTGGCTTGTCCTC+AGG 0.433345 4:+101247704 MsaT024592.1:CDS
CTCAGCCTCAAGAGCTAGGA+AGG 0.439395 4:+101248003 MsaT024592.1:CDS
AAGGGTGGTGACTGGTCCTC+AGG 0.440118 4:+101247584 MsaT024592.1:CDS
ATATGTGGCATCATGCTGTT+AGG 0.443627 4:+101245751 MsaT024592.1:CDS
CTCAGGCTCAAGAGCTAGGA+AGG 0.446172 4:+101247961 MsaT024592.1:CDS
AAGGGTGGTTGCTGCTCCAC+AGG 0.447691 4:+101247662 MsaT024592.1:CDS
CTCAGACTCGAAAGCTAAGA+AGG 0.449485 4:+101247799 MsaT024592.1:CDS
CAACAATATCCAATGGCATA+TGG 0.455357 4:+101246997 MsaT024592.1:CDS
AAATAAACATCAACAAGAAC+AGG 0.463567 4:+101246885 MsaT024592.1:CDS
AACACAAGATATACTAATTG+AGG 0.466931 4:+101248047 MsaT024592.1:CDS
CTCAGCCTCAAGAGCTAGGA+AGG 0.474289 4:+101247445 MsaT024592.1:CDS
TCAAGAGCTGGGAAGGGTGG+TGG 0.474375 4:+101247374 MsaT024592.1:CDS
CTCAGGTTCAAGAGCTGGGA+AGG 0.474845 4:+101247367 MsaT024592.1:CDS
CTCAGCCTCAAAAGCTAGGA+AGG 0.475835 4:+101247919 MsaT024592.1:CDS
CTCAACCTCAAAAGCTAGGA+AGG 0.475887 4:+101247685 MsaT024592.1:CDS
CTCAAGATCAAGAGCTAGGA+AGG 0.478408 4:+101247403 MsaT024592.1:CDS
CTCAACATCAAGAGCTAGGA+AGG 0.478408 4:+101247523 MsaT024592.1:CDS
CTCAGGCTCAAGAGCTAGGA+AGG 0.478532 4:+101247601 MsaT024592.1:CDS
CTCAACCTCAAGAGCTAGGA+AGG 0.478532 4:+101247643 MsaT024592.1:CDS
CTCAGGCTCAAGAGCTAGGA+AGG 0.478532 4:+101247721 MsaT024592.1:CDS
CTCAGGCTCAAGAGCTAGGA+AGG 0.478532 4:+101247835 MsaT024592.1:CDS
GATTGAGGGTAGCCAAATAA+GGG 0.479725 4:+101245821 MsaT024592.1:CDS
TAAAGAATGCGTACTGGAAG+TGG 0.480432 4:-101246024 None:intergenic
AACAATTCTTGAACATATGA+AGG 0.483635 4:+101246303 MsaT024592.1:CDS
TATATTTACTTACTAATGAC+AGG 0.483959 4:-101245539 None:intergenic
TGGTCCTCAGGTTCAAGAGC+TGG 0.485261 4:+101247362 MsaT024592.1:CDS
AATGAGCAATTGGGGATTGA+GGG 0.485917 4:+101245807 MsaT024592.1:CDS
TACAGCAGCATGTGAACTGT+TGG 0.487233 4:-101246932 None:intergenic
CAATGAGCAATTGGGGATTG+AGG 0.491122 4:+101245806 MsaT024592.1:CDS
CCATGATGCAGGTTCGTGTT+GGG 0.494378 4:-101247075 None:intergenic
TGTTCTGAAGGATCAACAAA+TGG 0.496320 4:+101245657 MsaT024592.1:CDS
GAAGAGAAAGAAGACGAAGA+AGG 0.499056 4:+101245935 MsaT024592.1:CDS
TTTCCCCTTCCTAGCTCTTG+AGG 0.499640 4:-101248008 None:intergenic
AGCTCTTGAGGCTGAGCTTG+AGG 0.500477 4:-101247558 None:intergenic
AGCTCTTGAGGCTGAGCTTG+AGG 0.500477 4:-101247756 None:intergenic
AAGGGTGGTGGCTACTCCAC+AGG 0.505062 4:+101247980 MsaT024592.1:CDS
TCCATGATGCAGGTTCGTGT+TGG 0.506466 4:-101247076 None:intergenic
ATCAACAAATGGAATAATGA+AGG 0.507137 4:+101245668 MsaT024592.1:CDS
CTTCCTAGCTCTTGATCTTG+AGG 0.508572 4:-101247402 None:intergenic
CGAAGAAGGAGCGGGTGGTA+TGG 0.509910 4:+101245949 MsaT024592.1:CDS
TGAAGCTGAAATGCCGGACA+TGG 0.511513 4:+101245472 MsaT024592.1:CDS
AAGAGCTAGGAAGGGGAAAG+AGG 0.511686 4:+101248012 MsaT024592.1:CDS
GAGCTAGGAATGGTGGTGGC+TGG 0.512918 4:+101247774 MsaT024592.1:CDS
TTCATCTGAGGAACATGCAT+TGG 0.513254 4:-101247243 None:intergenic
GGAGCAAGCTCTCATGGTTG+TGG 0.514199 4:+101246906 MsaT024592.1:CDS
GCCTAGACTTACAAGGGAGC+AGG 0.518181 4:+101246327 MsaT024592.1:CDS
AAAGAAGACGAAGAAGGAGC+GGG 0.518595 4:+101245941 MsaT024592.1:CDS
CAAGCTCAGCCTCAAGAGCT+AGG 0.520411 4:+101247561 MsaT024592.1:CDS
GGTCCTCAGGCTCAAGAGCT+AGG 0.520740 4:+101247477 MsaT024592.1:CDS
GGTCCTCAGGCTCAAGAGCT+AGG 0.520740 4:+101247597 MsaT024592.1:CDS
TGTCCTCAGGCTCAAGAGCT+AGG 0.520740 4:+101247717 MsaT024592.1:CDS
GGTCCTCAGGCTCAAGAGCT+AGG 0.520740 4:+101247831 MsaT024592.1:CDS
CAAGCTCAGCCTCAAAAGCT+AGG 0.520799 4:+101247915 MsaT024592.1:CDS
AGCTCTTGAGGCTGAGCCTG+TGG 0.521609 4:-101247996 None:intergenic
AGCTCTTGAGGTTGAGCATG+TGG 0.521665 4:-101247636 None:intergenic
CAGGCTCAGGCTCAAGAGCT+AGG 0.522151 4:+101247957 MsaT024592.1:CDS
CAAGCTCAGCCTCAAGAGCT+AGG 0.524513 4:+101247759 MsaT024592.1:CDS
AAAATCAAGTAAAGTATATG+TGG 0.524588 4:+101245736 MsaT024592.1:CDS
AGTTGGTCTTGGAAACTTGA+TGG 0.528215 4:+101247164 MsaT024592.1:CDS
AATCACAAGAACTTTAACAT+TGG 0.529369 4:-101245213 None:intergenic
CAGGCTCAGCCTCAAGAGCT+AGG 0.529994 4:+101247441 MsaT024592.1:CDS
CAGGCTCAGCCTCAAGAGCT+AGG 0.529994 4:+101247999 MsaT024592.1:CDS
AGAACAGGAGCAAGCTCTCA+TGG 0.536290 4:+101246900 MsaT024592.1:CDS
GAGCTAGGAAGGGTGGTGAC+TGG 0.538059 4:+101247576 MsaT024592.1:CDS
GCCTCAAGAGCTAGGAAGGA+TGG 0.540507 4:+101247449 MsaT024592.1:CDS
TCCACGATGTTGTCCACACT+CGG 0.540582 4:-101247288 None:intergenic
GAGCTAGGAAGGGTGGTGGC+TGG 0.541935 4:+101247534 MsaT024592.1:CDS
GAGCTAGGAAGGGTGGTGGC+TGG 0.541935 4:+101247732 MsaT024592.1:CDS
ACACAAGATATACTAATTGA+GGG 0.543203 4:+101248048 MsaT024592.1:CDS
GGTCCTCAGGTTCAAGAGCT+GGG 0.544277 4:+101247363 MsaT024592.1:CDS
TTCTTTGTCTTCTTTGTCCG+GGG 0.544906 4:-101245915 None:intergenic
AAATCAAATGAATCCATGTC+CGG 0.545425 4:-101245485 None:intergenic
AGCTCTTGAGGCTGAGCCTG+AGG 0.546926 4:-101247438 None:intergenic
AGCTCTTGAGCCTGAGCCTG+AGG 0.546926 4:-101247954 None:intergenic
ACATGCATTGGTGATTGTTG+TGG 0.548414 4:-101247231 None:intergenic
GGTTCAAGAGCTGGGAAGGG+TGG 0.548919 4:+101247371 MsaT024592.1:CDS
CCCAACACGAACCTGCATCA+TGG 0.549931 4:+101247075 MsaT024592.1:CDS
AAGCTAAGAAGGGTGGTGGC+TGG 0.555418 4:+101247810 MsaT024592.1:CDS
ATGTTGCATATCCATGATGC+AGG 0.557330 4:-101247086 None:intergenic
CAGGCTCAACATCAAGAGCT+AGG 0.558850 4:+101247519 MsaT024592.1:CDS
CAGGCTCAACCTCAAAAGCT+AGG 0.561577 4:+101247681 MsaT024592.1:CDS
TTTGTCTTCTTTGTCCGGGG+TGG 0.562381 4:-101245912 None:intergenic
CAGCCTCAAAAGCTAGGAAG+GGG 0.562802 4:+101247921 MsaT024592.1:CDS
CATGCTCAACCTCAAGAGCT+AGG 0.564867 4:+101247639 MsaT024592.1:CDS
AAGCTAGGAAGGGTGGTGGC+TGG 0.568049 4:+101247888 MsaT024592.1:CDS
GAAAGAAGACGAAGAAGGAG+CGG 0.568269 4:+101245940 MsaT024592.1:CDS
GGCTGATGAGGAAAATGCCT+TGG 0.570075 4:-101247054 None:intergenic
TTTCCTAGCTCTTGAGCCTG+AGG 0.573303 4:-101247480 None:intergenic
GAGCTAGGAAGGATGGCGGC+TGG 0.575246 4:+101247456 MsaT024592.1:CDS
TGAAGGTGCCTAGACTTACA+AGG 0.576956 4:+101246320 MsaT024592.1:CDS
ACCTGCTCCCTTGTAAGTCT+AGG 0.577890 4:-101246328 None:intergenic
GGCTCAAGAGCTAGGAAGGG+TGG 0.577942 4:+101247839 MsaT024592.1:CDS
TGCATCATGGATATGCAACA+TGG 0.578384 4:+101247088 MsaT024592.1:CDS
ACCACCCTTCCTAGCTCTTG+AGG 0.578654 4:-101247648 None:intergenic
GGCTCAAGAGCTAGGAAAGG+TGG 0.579566 4:+101247485 MsaT024592.1:CDS
GCCTCAAGAGCTAGGAATGG+TGG 0.580235 4:+101247767 MsaT024592.1:CDS
CAGCCTCAAGAGCTAGGAAG+GGG 0.581740 4:+101248005 MsaT024592.1:CDS
GGTCCTCAAGATCAAGAGCT+AGG 0.581757 4:+101247399 MsaT024592.1:CDS
TTCCCAGAAAATTGATACCA+AGG 0.582328 4:+101247037 MsaT024592.1:CDS
ACCACCCTTCCTAGCTCTTG+AGG 0.582587 4:-101247570 None:intergenic
GCCTCAAGAGCTAGGAAGGG+TGG 0.582674 4:+101247569 MsaT024592.1:CDS
GGCTCAAGAGCTAGGAAGGG+TGG 0.582674 4:+101247605 MsaT024592.1:CDS
GGCTCAAGAGCTAGGAAGGG+TGG 0.582674 4:+101247725 MsaT024592.1:CDS
GGCTCAAGAGCTAGGAAGGG+TGG 0.582674 4:+101247965 MsaT024592.1:CDS
CTTCCTAGCTCTTGAGCCTG+AGG 0.585618 4:-101247600 None:intergenic
CTTCCTAGCTCTTGAGCCTG+AGG 0.585618 4:-101247834 None:intergenic
ACTACTTGAAGCTGAAATGC+CGG 0.587583 4:+101245466 MsaT024592.1:CDS
GAGCTAGGAAGGGTGGTGGT+TGG 0.591794 4:+101247414 MsaT024592.1:CDS
TCAAAAGCTAGGAAGGGTGG+TGG 0.595324 4:+101247692 MsaT024592.1:CDS
TTATGATGTTCTTGTTGCTG+AGG 0.596660 4:-101247207 None:intergenic
TGGTCTCAATTCAATTACCA+AGG 0.596923 4:+101245275 MsaT024592.1:CDS
GCCTCAAAAGCTAGGAAGGG+GGG 0.598080 4:+101247923 MsaT024592.1:CDS
GCTGTTAGGAAAATGATGAA+AGG 0.599623 4:+101245765 MsaT024592.1:CDS
ACGCTTAAACGATGTACCAT+CGG 0.602369 4:+101245863 MsaT024592.1:CDS
GAACTGCAACCATATGCCAT+TGG 0.602482 4:-101247006 None:intergenic
CATGATCATGATCATGCAGT+TGG 0.605467 4:+101247147 MsaT024592.1:CDS
TCAGCCTCAAAAGCTAGGAA+GGG 0.606989 4:+101247920 MsaT024592.1:CDS
GCTACAATGCAAGTGGAGGT+TGG 0.607441 4:+101247342 MsaT024592.1:CDS
GCCATCCTTCCTAGCTCTTG+AGG 0.607945 4:-101247450 None:intergenic
TCGAAAGCTAAGAAGGGTGG+TGG 0.609238 4:+101247806 MsaT024592.1:CDS
ACCTCAAAAGCTAGGAAGGG+TGG 0.610885 4:+101247689 MsaT024592.1:CDS
CGGTGAAGCTACAATGCAAG+TGG 0.610968 4:+101247335 MsaT024592.1:CDS
CTTCCCAGCTCTTGAACCTG+AGG 0.611183 4:-101247366 None:intergenic
TTGACTGCATTATTCATCTG+AGG 0.611554 4:-101247255 None:intergenic
TCAAGAGCTAGGAAGGGTGG+TGG 0.612518 4:+101247530 MsaT024592.1:CDS
TCAGTTATATAATATTCACT+TGG 0.614757 4:-101247123 None:intergenic
GACTCGAAAGCTAAGAAGGG+TGG 0.616097 4:+101247803 MsaT024592.1:CDS
ACCACCATTCCTAGCTCTTG+AGG 0.617365 4:-101247768 None:intergenic
ACCTCAAGAGCTAGGAAGGG+TGG 0.617706 4:+101247647 MsaT024592.1:CDS
TCAGCCTCAAGAGCTAGGAA+GGG 0.618998 4:+101248004 MsaT024592.1:CDS
TCAAGAGCTAGGAAGGGTGG+TGG 0.619733 4:+101247410 MsaT024592.1:CDS
CTTCCTAGCTCTTGAGCCTG+AGG 0.620806 4:-101247720 None:intergenic
TCAGGCTCAAGAGCTAGGAA+GGG 0.624572 4:+101247962 MsaT024592.1:CDS
AAGATATACCCGAACCACCC+CGG 0.626205 4:+101245898 MsaT024592.1:CDS
TGCAGAACAACAATATCCAA+TGG 0.629974 4:+101246990 MsaT024592.1:CDS
GGCTGGAAACTCATTCCCAA+AGG 0.633277 4:-101245192 None:intergenic
CAATGATGTTGTACCGAGTG+TGG 0.635078 4:+101247275 MsaT024592.1:CDS
TCAGGTTCAAGAGCTGGGAA+GGG 0.636064 4:+101247368 MsaT024592.1:CDS
TCAAGAGCTAGGAAGGGTGG+TGG 0.636286 4:+101247608 MsaT024592.1:CDS
TCAAGAGCTAGGAAGGGTGG+TGG 0.636286 4:+101247728 MsaT024592.1:CDS
TCAAGAGCTAGGAAAGGTGG+TGG 0.637451 4:+101247488 MsaT024592.1:CDS
TCAAGAGCTAGGAATGGTGG+TGG 0.637451 4:+101247770 MsaT024592.1:CDS
TCAACCTCAAAAGCTAGGAA+GGG 0.637506 4:+101247686 MsaT024592.1:CDS
TCAAGAGCTAGGAAGGGTGG+TGG 0.638725 4:+101247968 MsaT024592.1:CDS
TCAGACTCGAAAGCTAAGAA+GGG 0.638931 4:+101247800 MsaT024592.1:CDS
AGCCTCAAAAGCTAGGAAGG+GGG 0.642823 4:+101247922 MsaT024592.1:CDS
TCAGCCTCAAGAGCTAGGAA+TGG 0.643593 4:+101247764 MsaT024592.1:CDS
TCAATGCTATTGAGAAAACA+TGG 0.646799 4:+101245255 MsaT024592.1:CDS
AGATCAAGAGCTAGGAAGGG+TGG 0.650678 4:+101247407 MsaT024592.1:CDS
ACATCAAGAGCTAGGAAGGG+TGG 0.650678 4:+101247527 MsaT024592.1:CDS
TCAGCCTCAAGAGCTAGGAA+GGG 0.652611 4:+101247566 MsaT024592.1:CDS
AGGTTCGTGTTGGGCTGATG+AGG 0.653657 4:-101247066 None:intergenic
TCAGGCTCAAGAGCTAGGAA+AGG 0.654595 4:+101247482 MsaT024592.1:CDS
GCATCATGGATATGCAACAT+GGG 0.656904 4:+101247089 MsaT024592.1:CDS
GAAGGTGCCTAGACTTACAA+GGG 0.661563 4:+101246321 MsaT024592.1:CDS
TGAAGCTACAATGCAAGTGG+AGG 0.663678 4:+101247338 MsaT024592.1:CDS
GAAGACGAAGAAGGAGCGGG+TGG 0.670517 4:+101245944 MsaT024592.1:CDS
ACCGAGTGTGGACAACATCG+TGG 0.681310 4:+101247287 MsaT024592.1:CDS
TCAGGCTCAAGAGCTAGGAA+GGG 0.685952 4:+101247602 MsaT024592.1:CDS
TCAGGCTCAAGAGCTAGGAA+GGG 0.685952 4:+101247722 MsaT024592.1:CDS
TCAGGCTCAAGAGCTAGGAA+GGG 0.685952 4:+101247836 MsaT024592.1:CDS
GCAGTGACGATGTGTTCTGA+AGG 0.690924 4:+101245645 MsaT024592.1:intron
TCAACATCAAGAGCTAGGAA+GGG 0.691703 4:+101247524 MsaT024592.1:CDS
TCAACCTCAAGAGCTAGGAA+GGG 0.693140 4:+101247644 MsaT024592.1:CDS
TCAAGATCAAGAGCTAGGAA+GGG 0.696103 4:+101247404 MsaT024592.1:CDS
CTGTATTGAAATCATCACAG+TGG 0.740386 4:+101246950 MsaT024592.1:CDS
TCAAGAGCTAGGAAGGATGG+CGG 0.754981 4:+101247452 MsaT024592.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 101245182 101248067 101245182 ID=MsaG024592
Chr4 mRNA 101245182 101248067 101245182 ID=MsaT024592.1;Parent=MsaG024592
Chr4 exon 101245182 101245296 101245182 ID=MsaT024592.1.exon1;Parent=MsaT024592.1
Chr4 CDS 101245182 101245296 101245182 ID=cds.MsaT024592.1;Parent=MsaT024592.1
Chr4 exon 101245399 101245548 101245399 ID=MsaT024592.1.exon2;Parent=MsaT024592.1
Chr4 CDS 101245399 101245548 101245399 ID=cds.MsaT024592.1;Parent=MsaT024592.1
Chr4 exon 101245649 101246032 101245649 ID=MsaT024592.1.exon3;Parent=MsaT024592.1
Chr4 CDS 101245649 101246032 101245649 ID=cds.MsaT024592.1;Parent=MsaT024592.1
Chr4 exon 101246290 101246366 101246290 ID=MsaT024592.1.exon4;Parent=MsaT024592.1
Chr4 CDS 101246290 101246366 101246290 ID=cds.MsaT024592.1;Parent=MsaT024592.1
Chr4 exon 101246868 101248067 101246868 ID=MsaT024592.1.exon5;Parent=MsaT024592.1
Chr4 CDS 101246868 101248067 101246868 ID=cds.MsaT024592.1;Parent=MsaT024592.1
Gene Sequence

>MsaT024592.1

ATGGATTTTTCCTTTGGGAATGAGTTTCCAGCCAATGTTAAAGTTCTTGTGATTGATCATGATATCGATCTTCTCAATGCTATTGAGAAAACATGGTCTCAATTCAATTACCAAGTTACTACATGTTCGACAGTTTCTTCAGCCTTGAATTTGATAGCACAAAAGGTGCATTTTGATTTGGTACTACTTGAAGCTGAAATGCCGGACATGGATTCATTTGATTTCTTGCAAAAAATTACACAACAAATCGATATTCCTGTCATTATGACGATGTGTTCTGAAGGATCAACAAATGGAATAATGAAGGCAATCGAAAATGGAGCTTGTGACTGTTTTGTCAAGCCATTCACTGAAAATCAAGTAAAGTATATGTGGCATCATGCTGTTAGGAAAATGATGAAAGGAAATAAAAAACAAAAAATCAATGAGCAATTGGGGATTGAGGGTAGCCAAATAAGGGCAAGAGATGATTCAAATTTACGCTTAAACGATGTACCATCGGAAAAAGGTGACGAAGATATACCCGAACCACCCCGGACAAAGAAGACAAAGAAGAGAAAGAAGACGAAGAAGGAGCGGGTGGTATGGTCTTTAGAATTACATGAGAAATTCATGAATGCTGTTAAAGAACTTGATGCTTCCACTTCCAAGGCGGTACCAAAAACAATTCTTGAACATATGAAGGTGCCTAGACTTACAAGGGAGCAGGTAGCTAGTCATCTTCAGAAGTATAGACTGTATTTAAATAAACATCAACAAGAACAGGAGCAAGCTCTCATGGTTGTGGAATCCAACAGTTCACATGCTGCTGTATTGAAATCATCACAGTGGTCTCACAATTCTCATGTTGCAGAACAACAATATCCAATGGCATATGGTTGCAGTTCTCAACAAGTTCCCAGAAAATTGATACCAAGGCATTTTCCTCATCAGCCCAACACGAACCTGCATCATGGATATGCAACATGGGAGCAGCAATTTCCAAGTGAATATTATATAACTGATCATGATCATGATCATGCAGTTGGTCTTGGAAACTTGATGGCGAATAATAGTAATGCATTGCCTCAGCAACAAGAACATCATAATCCACAACAATCACCAATGCATGTTCCTCAGATGAATAATGCAGTCAATGATGTTGTACCGAGTGTGGACAACATCGTGGAAGTGAATAATACTGTAACAGATTTCGGTGAAGCTACAATGCAAGTGGAGGTTGGTCCTCAGGTTCAAGAGCTGGGAAGGGTGGTGGCTGGTCCTCAAGATCAAGAGCTAGGAAGGGTGGTGGTTGGTCCTCAGGCTCAGCCTCAAGAGCTAGGAAGGATGGCGGCTGGTCCTCAGGCTCAAGAGCTAGGAAAGGTGGTGGCTGCTCGTCAGGCTCAACATCAAGAGCTAGGAAGGGTGGTGGCTGGTCCTCAAGCTCAGCCTCAAGAGCTAGGAAGGGTGGTGACTGGTCCTCAGGCTCAAGAGCTAGGAAGGGTGGTGGCTGCTCCACATGCTCAACCTCAAGAGCTAGGAAGGGTGGTTGCTGCTCCACAGGCTCAACCTCAAAAGCTAGGAAGGGTGGTGGCTTGTCCTCAGGCTCAAGAGCTAGGAAGGGTGGTGGCTGGTCCTCAAGCTCAGCCTCAAGAGCTAGGAATGGTGGTGGCTGGTTCTCAGACTCGAAAGCTAAGAAGGGTGGTGGCTGGTCCTCAGGCTCAAGAGCTAGGAAGGGTGGCTGCTGCTCCACATGCTCAACCTAAAAAGCTAGGAAGGGTGGTGGCTGGTCCCCAAGCTCAGCCTCAAAAGCTAGGAAGGGGGGTGACTGGTCCTCAGGCTCAGGCTCAAGAGCTAGGAAGGGTGGTGGCTACTCCACAGGCTCAGCCTCAAGAGCTAGGAAGGGGAAAGAGGTATAAGAAGCCTAACACAAGATATACTAATTGA
Protein sequence

>MsaT024592.1

MDFSFGNEFPANVKVLVIDHDIDLLNAIEKTWSQFNYQVTTCSTVSSALNLIAQKVHFDLVLLEAEMPDMDSFDFLQKITQQIDIPVIMTMCSEGSTNGIMKAIENGACDCFVKPFTENQVKYMWHHAVRKMMKGNKKQKINEQLGIEGSQIRARDDSNLRLNDVPSEKGDEDIPEPPRTKKTKKRKKTKKERVVWSLELHEKFMNAVKELDASTSKAVPKTILEHMKVPRLTREQVASHLQKYRLYLNKHQQEQEQALMVVESNSSHAAVLKSSQWSHNSHVAEQQYPMAYGCSSQQVPRKLIPRHFPHQPNTNLHHGYATWEQQFPSEYYITDHDHDHAVGLGNLMANNSNALPQQQEHHNPQQSPMHVPQMNNAVNDVVPSVDNIVEVNNTVTDFGEATMQVEVGPQVQELGRVVAGPQDQELGRVVVGPQAQPQELGRMAAGPQAQELGKVVAARQAQHQELGRVVAGPQAQPQELGRVVTGPQAQELGRVVAAPHAQPQELGRVVAAPQAQPQKLGRVVACPQAQELGRVVAGPQAQPQELGMVVAGSQTRKLRRVVAGPQAQELGRVAAAPHAQPKKLGRVVAGPQAQPQKLGRGVTGPQAQAQELGRVVATPQAQPQELGRGKRYKKPNTRYTN*