Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024685 | XP_003593904.2 | 85.263 | 95 | 13 | 1 | 1 | 94 | 40 | 134 | 2.10e-49 | 162 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024685 | sp|Q8LBM2|TIF5A_ARATH | 44.565 | 92 | 44 | 3 | 4 | 93 | 42 | 128 | 8.33e-16 | 70.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024685 | tr|G7IP70|G7IP70_MEDTR | 85.263 | 95 | 13 | 1 | 1 | 94 | 40 | 134 | 1.00e-49 | 162 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG024685 | TF | Tify |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024685 | MtrunA17_Chr2g0285821 | 85.263 | 95 | 13 | 1 | 1 | 94 | 40 | 134 | 1.93e-53 | 162 |
| MsaG024685 | MtrunA17_Chr8g0393051 | 84.043 | 94 | 4 | 2 | 1 | 94 | 36 | 118 | 2.05e-47 | 147 |
| MsaG024685 | MtrunA17_Chr8g0376421 | 58.696 | 92 | 32 | 2 | 2 | 93 | 39 | 124 | 1.37e-27 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024685 | AT1G30135.1 | 44.565 | 92 | 44 | 3 | 4 | 93 | 42 | 128 | 8.47e-17 | 70.1 |
| MsaG024685 | AT2G34600.1 | 42.857 | 91 | 49 | 3 | 4 | 93 | 57 | 145 | 9.24e-16 | 68.2 |
Find 23 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTGATGTCACAGAGTTTC+AGG | 0.192920 | 4:-102221026 | MsaT024685.1:intron |
| TGCCAGCATTAAGATGGATT+TGG | 0.344981 | 4:+102220892 | None:intergenic |
| CATCTTCCCATCATAGAATA+TGG | 0.389620 | 4:+102221054 | None:intergenic |
| CCTCATCAATTGTATAGCCC+TGG | 0.405940 | 4:-102220794 | MsaT024685.1:CDS |
| GATGGTATGGAGATGCTTCT+TGG | 0.408900 | 4:+102220711 | None:intergenic |
| TCTACAGAAGAGAAAATATA+GGG | 0.416172 | 4:-102220735 | MsaT024685.1:CDS |
| GGAAAGCCCAATGCAAAATC+AGG | 0.420902 | 4:-102221089 | None:intergenic |
| TTTATTTGCCAGCATTAAGA+TGG | 0.422633 | 4:+102220886 | None:intergenic |
| CCGCTGACCATATTCTATGA+TGG | 0.457336 | 4:-102221061 | MsaT024685.1:CDS |
| TTCTACAGAAGAGAAAATAT+AGG | 0.461958 | 4:-102220736 | MsaT024685.1:CDS |
| CTCATCAATTGTATAGCCCT+GGG | 0.470700 | 4:-102220793 | MsaT024685.1:CDS |
| TGAGGAGGCTGCACTATTGT+TGG | 0.477785 | 4:+102220812 | None:intergenic |
| CAATTGTATAGCCCTGGGTC+TGG | 0.482257 | 4:-102220788 | MsaT024685.1:CDS |
| CGCTGACCATATTCTATGAT+GGG | 0.509614 | 4:-102221060 | MsaT024685.1:CDS |
| GGCCAAATCCATCTTAATGC+TGG | 0.519970 | 4:-102220894 | MsaT024685.1:CDS |
| GCAAAGATCTCTTCATTGAA+AGG | 0.520559 | 4:+102220765 | None:intergenic |
| TTCATTGAAAGGCCAGACCC+AGG | 0.532257 | 4:+102220776 | None:intergenic |
| GTTGTTCGCTCTGACCCTCT+CGG | 0.542596 | 4:+102220836 | None:intergenic |
| CAATGAAGAGATCTTTGCAA+CGG | 0.550495 | 4:-102220760 | MsaT024685.1:CDS |
| GGGCTATACAATTGATGAGG+AGG | 0.602793 | 4:+102220797 | None:intergenic |
| TCATTGAAAGGCCAGACCCA+GGG | 0.615225 | 4:+102220777 | None:intergenic |
| CCAGGGCTATACAATTGATG+AGG | 0.668057 | 4:+102220794 | None:intergenic |
| CCATCATAGAATATGGTCAG+CGG | 0.671722 | 4:+102221061 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 102220706 | 102221101 | 102220706 | ID=MsaG024685 |
| Chr4 | mRNA | 102220706 | 102221101 | 102220706 | ID=MsaT024685.1;Parent=MsaG024685 |
| Chr4 | exon | 102220706 | 102220915 | 102220706 | ID=MsaT024685.1.exon2;Parent=MsaT024685.1 |
| Chr4 | CDS | 102220706 | 102220915 | 102220706 | ID=cds.MsaT024685.1;Parent=MsaT024685.1 |
| Chr4 | exon | 102221027 | 102221101 | 102221027 | ID=MsaT024685.1.exon1;Parent=MsaT024685.1 |
| Chr4 | CDS | 102221027 | 102221101 | 102221027 | ID=cds.MsaT024685.1;Parent=MsaT024685.1 |
| Gene Sequence |
| Protein sequence |
