Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024842 | AES93696.1 | 84.021 | 194 | 26 | 4 | 1 | 190 | 1 | 193 | 3.31e-107 | 315 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024842 | sp|P05332|YP20_BACLI | 31.492 | 181 | 111 | 5 | 15 | 188 | 2 | 176 | 2.36e-18 | 81.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024842 | tr|G7K3P3|G7K3P3_MEDTR | 84.021 | 194 | 26 | 4 | 1 | 190 | 1 | 193 | 1.58e-107 | 315 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG024842 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024842 | MtrunA17_Chr5g0394631 | 80.829 | 193 | 29 | 4 | 1 | 190 | 214 | 401 | 1.23e-101 | 298 |
| MsaG024842 | MtrunA17_Chr5g0394631 | 67.582 | 182 | 57 | 2 | 1 | 181 | 1 | 181 | 1.56e-79 | 242 |
| MsaG024842 | MtrunA17_Chr5g0394621 | 71.354 | 192 | 51 | 3 | 1 | 190 | 1 | 190 | 4.88e-90 | 261 |
| MsaG024842 | MtrunA17_Chr1g0204951 | 59.794 | 194 | 70 | 5 | 1 | 190 | 1 | 190 | 1.97e-73 | 219 |
| MsaG024842 | MtrunA17_Chr1g0204971 | 59.278 | 194 | 71 | 5 | 1 | 190 | 1 | 190 | 4.88e-73 | 218 |
| MsaG024842 | MtrunA17_Chr7g0223361 | 42.442 | 172 | 95 | 2 | 16 | 187 | 3 | 170 | 1.73e-38 | 130 |
| MsaG024842 | MtrunA17_Chr7g0223351 | 41.420 | 169 | 92 | 5 | 20 | 187 | 7 | 169 | 5.91e-36 | 124 |
| MsaG024842 | MtrunA17_Chr7g0273661 | 40.385 | 156 | 90 | 1 | 17 | 172 | 4 | 156 | 6.56e-34 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024842 | AT2G32020.1 | 52.047 | 171 | 71 | 6 | 20 | 187 | 18 | 180 | 1.36e-48 | 156 |
| MsaG024842 | AT2G32030.1 | 50.588 | 170 | 73 | 6 | 21 | 187 | 24 | 185 | 1.53e-48 | 156 |
| MsaG024842 | AT3G22560.1 | 36.571 | 175 | 104 | 3 | 16 | 187 | 2 | 172 | 2.13e-30 | 110 |
Find 38 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAAGTATGTATTCTTTAA+AGG | 0.161729 | 5:-1390315 | MsaT024842.1:CDS |
| AAAATCAAATGTGCAGAAAT+TGG | 0.173070 | 5:-1390519 | MsaT024842.1:CDS |
| ATGAAAAGGTAGCCAAGTTC+TGG | 0.273400 | 5:-1390686 | MsaT024842.1:CDS |
| CTCTTCATTGGCACATGGTT+TGG | 0.274557 | 5:+1390788 | None:intergenic |
| GCTTCAAGCCTTTCCAAGTA+TGG | 0.276638 | 5:+1390411 | None:intergenic |
| ATGTTCTAGGCTCTAAATAT+TGG | 0.292142 | 5:-1390494 | MsaT024842.1:CDS |
| TGTTCTAGGCTCTAAATATT+GGG | 0.316974 | 5:-1390493 | MsaT024842.1:CDS |
| TTGTTGATGTGGATAATTTA+TGG | 0.328127 | 5:-1390386 | MsaT024842.1:CDS |
| TGCCATAATGATCATGCTAT+TGG | 0.333891 | 5:-1390582 | MsaT024842.1:CDS |
| ACCCAATAGCATGATCATTA+TGG | 0.341789 | 5:+1390580 | None:intergenic |
| GCAGAAATTGGGTATGTTCT+AGG | 0.344662 | 5:-1390507 | MsaT024842.1:CDS |
| CAAATCAATGATCTCTTCAT+TGG | 0.363988 | 5:+1390776 | None:intergenic |
| CCTTCGTCTTTGCTAGTGTA+AGG | 0.376096 | 5:+1390657 | None:intergenic |
| GGCTCTAAATATTGGGGTAA+AGG | 0.406739 | 5:-1390486 | MsaT024842.1:CDS |
| ATTCAGTGAATTTCCATACT+TGG | 0.413337 | 5:-1390424 | MsaT024842.1:CDS |
| CACGTTTGCTGTGAAACAAA+TGG | 0.422199 | 5:-1390457 | MsaT024842.1:CDS |
| GCTCTAAATATTGGGGTAAA+GGG | 0.428389 | 5:-1390485 | MsaT024842.1:CDS |
| TTCTGACCTTGATGATGTAA+TGG | 0.440976 | 5:-1390724 | MsaT024842.1:CDS |
| CAAAGAGTAGTTGAGAAAGT+TGG | 0.450786 | 5:-1390360 | MsaT024842.1:CDS |
| GAAGATGTCGGAGTGTGATT+TGG | 0.453912 | 5:+1390751 | None:intergenic |
| AAATCAAATGTGCAGAAATT+GGG | 0.456981 | 5:-1390518 | MsaT024842.1:CDS |
| TGCTGTGAAACAAATGGTTA+AGG | 0.473285 | 5:-1390451 | MsaT024842.1:CDS |
| GCCATAATGATCATGCTATT+GGG | 0.478546 | 5:-1390581 | MsaT024842.1:CDS |
| TGAAAAGGTAGCCAAGTTCT+GGG | 0.482152 | 5:-1390685 | MsaT024842.1:CDS |
| GTGAATTTCCATACTTGGAA+AGG | 0.490341 | 5:-1390419 | MsaT024842.1:CDS |
| GCTTGAAGCTCTTGTTGATG+TGG | 0.580383 | 5:-1390397 | MsaT024842.1:CDS |
| GTGGCAGCAGACTGATGAAA+AGG | 0.594266 | 5:-1390700 | MsaT024842.1:CDS |
| GCCACACCATTACATCATCA+AGG | 0.594693 | 5:+1390718 | None:intergenic |
| ATGATCTCTTCATTGGCACA+TGG | 0.598564 | 5:+1390783 | None:intergenic |
| AAAAGGTAGCCAAGTTCTGG+GGG | 0.620200 | 5:-1390683 | MsaT024842.1:CDS |
| GAAAAGGTAGCCAAGTTCTG+GGG | 0.624494 | 5:-1390684 | MsaT024842.1:CDS |
| GTTCTAGGCTCTAAATATTG+GGG | 0.628624 | 5:-1390492 | MsaT024842.1:CDS |
| GGTCAGAAAGATGAAGATGT+CGG | 0.630507 | 5:+1390739 | None:intergenic |
| TAGATCATGACGTGAACTCG+AGG | 0.652639 | 5:+1390545 | None:intergenic |
| CATTTGTTTCACAGCAAACG+TGG | 0.659491 | 5:+1390458 | None:intergenic |
| ACCTTGATGATGTAATGGTG+TGG | 0.697032 | 5:-1390719 | MsaT024842.1:CDS |
| CCTTACACTAGCAAAGACGA+AGG | 0.715657 | 5:-1390657 | MsaT024842.1:CDS |
| AAAGGTAGCCAAGTTCTGGG+GGG | 0.779565 | 5:-1390682 | MsaT024842.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 1390260 | 1390832 | 1390260 | ID=MsaG024842 |
| Chr5 | mRNA | 1390260 | 1390832 | 1390260 | ID=MsaT024842.1;Parent=MsaG024842 |
| Chr5 | exon | 1390260 | 1390832 | 1390260 | ID=MsaT024842.1.exon1;Parent=MsaT024842.1 |
| Chr5 | CDS | 1390260 | 1390832 | 1390260 | ID=cds.MsaT024842.1;Parent=MsaT024842.1 |
| Gene Sequence |
| Protein sequence |
