Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024843 | XP_039690105.1 | 93.617 | 188 | 12 | 0 | 1 | 188 | 1 | 188 | 1.10e-127 | 367 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024843 | sp|P05332|YP20_BACLI | 33.143 | 175 | 106 | 4 | 15 | 182 | 2 | 172 | 1.60e-19 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024843 | tr|G7K3P4|G7K3P4_MEDTR | 93.617 | 188 | 12 | 0 | 1 | 188 | 1 | 188 | 5.24e-128 | 367 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG024843 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024843 | MtrunA17_Chr5g0394621 | 78.947 | 190 | 38 | 1 | 1 | 188 | 1 | 190 | 1.12e-104 | 298 |
| MsaG024843 | MtrunA17_Chr5g0394631 | 75.691 | 181 | 42 | 1 | 1 | 179 | 1 | 181 | 7.39e-95 | 281 |
| MsaG024843 | MtrunA17_Chr5g0394631 | 76.503 | 183 | 35 | 5 | 12 | 188 | 221 | 401 | 5.29e-90 | 269 |
| MsaG024843 | MtrunA17_Chr1g0204951 | 65.263 | 190 | 64 | 1 | 1 | 188 | 1 | 190 | 9.84e-87 | 253 |
| MsaG024843 | MtrunA17_Chr1g0204971 | 63.158 | 190 | 68 | 1 | 1 | 188 | 1 | 190 | 7.43e-85 | 248 |
| MsaG024843 | MtrunA17_Chr7g0223361 | 43.529 | 170 | 94 | 2 | 16 | 185 | 3 | 170 | 1.07e-42 | 140 |
| MsaG024843 | MtrunA17_Chr7g0273661 | 43.506 | 154 | 86 | 1 | 17 | 170 | 4 | 156 | 7.58e-41 | 135 |
| MsaG024843 | MtrunA17_Chr7g0223351 | 41.566 | 166 | 94 | 2 | 20 | 185 | 7 | 169 | 8.99e-39 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024843 | AT2G32020.1 | 54.491 | 167 | 71 | 3 | 20 | 185 | 18 | 180 | 2.99e-57 | 178 |
| MsaG024843 | AT2G32030.1 | 51.381 | 181 | 75 | 4 | 6 | 185 | 17 | 185 | 1.66e-56 | 177 |
| MsaG024843 | AT3G22560.1 | 38.953 | 172 | 102 | 2 | 16 | 185 | 2 | 172 | 3.30e-36 | 124 |
Find 38 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATCAATGATCTCTTCTT+TGG | 0.196407 | 5:+1393003 | None:intergenic |
| GGGAGTTCTTAGGAAGTATT+TGG | 0.241791 | 5:-1392558 | MsaT024843.1:CDS |
| CTCTTCTTTGGCACATGGTT+TGG | 0.246122 | 5:+1393015 | None:intergenic |
| ATAATCTAAGCTCTATATAT+TGG | 0.258828 | 5:-1392727 | MsaT024843.1:CDS |
| TCCTAAGAACTCCCTCCTTT+TGG | 0.260104 | 5:+1392567 | None:intergenic |
| ATCAAGGTCAGAAAGATTAA+AGG | 0.290557 | 5:+1392961 | None:intergenic |
| TAATCTAAGCTCTATATATT+GGG | 0.291812 | 5:-1392726 | MsaT024843.1:CDS |
| GTTGATGCCATCTTCTTTGC+TGG | 0.294680 | 5:+1392877 | None:intergenic |
| AGGTTCCCATGAGCAGAATT+TGG | 0.303996 | 5:+1392904 | None:intergenic |
| TGCTGTGAAACAAATTATTA+AGG | 0.312252 | 5:-1392684 | MsaT024843.1:CDS |
| CCATCTTCTTTGCTGGTGTA+AGG | 0.323803 | 5:+1392884 | None:intergenic |
| AAAATGAAACTTATTAAAGT+TGG | 0.351635 | 5:+1392480 | None:intergenic |
| TCTCCAAGTATGAAAATTCA+TGG | 0.373432 | 5:+1392654 | None:intergenic |
| TCCAAAAGGAGGGAGTTCTT+AGG | 0.394554 | 5:-1392568 | MsaT024843.1:CDS |
| TAAAGGGTCGGAGTGTGATT+TGG | 0.396804 | 5:+1392978 | None:intergenic |
| TGCATTGATGATCGTGCTAT+TGG | 0.407515 | 5:-1392809 | MsaT024843.1:CDS |
| AGGAAACAATCTGCAGAACT+TGG | 0.436712 | 5:-1392752 | MsaT024843.1:CDS |
| TTCTGACCTTGATGATGTAA+TGG | 0.440976 | 5:-1392951 | MsaT024843.1:CDS |
| TCAAGGTCAGAAAGATTAAA+GGG | 0.477441 | 5:+1392962 | None:intergenic |
| CTTGTCGATGTTGAAAATGT+TGG | 0.484592 | 5:-1392620 | MsaT024843.1:CDS |
| GCTCTATATATTGGGGCAAA+GGG | 0.493310 | 5:-1392718 | MsaT024843.1:CDS |
| CAAAGAGTGTTGGAGAAAGC+TGG | 0.499760 | 5:-1392593 | MsaT024843.1:CDS |
| AGCTCTATATATTGGGGCAA+AGG | 0.501996 | 5:-1392719 | MsaT024843.1:CDS |
| TTGTCGATGTTGAAAATGTT+GGG | 0.503194 | 5:-1392619 | MsaT024843.1:CDS |
| AAGTGGCCAAATTCTGCTCA+TGG | 0.506987 | 5:-1392910 | MsaT024843.1:CDS |
| AGGAAGTATTTGGTCATGAA+AGG | 0.520639 | 5:-1392548 | MsaT024843.1:CDS |
| ATGATCTCTTCTTTGGCACA+TGG | 0.535452 | 5:+1393010 | None:intergenic |
| GGAGAAAGCTGGTTTCCAAA+AGG | 0.547990 | 5:-1392582 | MsaT024843.1:CDS |
| CCTTACACCAGCAAAGAAGA+TGG | 0.560893 | 5:-1392884 | MsaT024843.1:CDS |
| GAAAGCTGGTTTCCAAAAGG+AGG | 0.562242 | 5:-1392579 | MsaT024843.1:CDS |
| TGTTGGGTCACAAAGAGTGT+TGG | 0.566749 | 5:-1392603 | MsaT024843.1:CDS |
| AGTGGCCAAATTCTGCTCAT+GGG | 0.587068 | 5:-1392909 | MsaT024843.1:CDS |
| AATCTAAGCTCTATATATTG+GGG | 0.593409 | 5:-1392725 | MsaT024843.1:CDS |
| GCCACACCATTACATCATCA+AGG | 0.594693 | 5:+1392945 | None:intergenic |
| GGTCAGAAAGATTAAAGGGT+CGG | 0.596865 | 5:+1392966 | None:intergenic |
| TCACACCTGAAGATAAAAGC+AGG | 0.598275 | 5:-1392772 | MsaT024843.1:CDS |
| AAAGCTGGTTTCCAAAAGGA+GGG | 0.657724 | 5:-1392578 | MsaT024843.1:CDS |
| ACCTTGATGATGTAATGGTG+TGG | 0.686805 | 5:-1392946 | MsaT024843.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 1392493 | 1393059 | 1392493 | ID=MsaG024843 |
| Chr5 | mRNA | 1392493 | 1393059 | 1392493 | ID=MsaT024843.1;Parent=MsaG024843 |
| Chr5 | exon | 1392493 | 1393059 | 1392493 | ID=MsaT024843.1.exon1;Parent=MsaT024843.1 |
| Chr5 | CDS | 1392493 | 1393059 | 1392493 | ID=cds.MsaT024843.1;Parent=MsaT024843.1 |
| Gene Sequence |
| Protein sequence |
