Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024845 | XP_024639963.1 | 92.632 | 190 | 14 | 0 | 1 | 190 | 1 | 190 | 9.71e-128 | 367 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024845 | sp|P05332|YP20_BACLI | 32.353 | 170 | 104 | 3 | 21 | 184 | 8 | 172 | 1.25e-19 | 84.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024845 | tr|A0A396HL91|A0A396HL91_MEDTR | 68.557 | 194 | 51 | 5 | 1 | 190 | 214 | 401 | 2.22e-78 | 248 |
| MsaG024845 | tr|A0A396HL91|A0A396HL91_MEDTR | 92.818 | 181 | 13 | 0 | 1 | 181 | 1 | 181 | 3.69e-119 | 352 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG024845 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024845 | MtrunA17_Chr5g0394631 | 92.818 | 181 | 13 | 0 | 1 | 181 | 1 | 181 | 7.10e-123 | 352 |
| MsaG024845 | MtrunA17_Chr5g0394631 | 68.557 | 194 | 51 | 5 | 1 | 190 | 214 | 401 | 4.28e-82 | 248 |
| MsaG024845 | MtrunA17_Chr5g0394621 | 78.421 | 190 | 41 | 0 | 1 | 190 | 1 | 190 | 1.18e-107 | 306 |
| MsaG024845 | MtrunA17_Chr1g0204951 | 61.458 | 192 | 70 | 2 | 1 | 190 | 1 | 190 | 4.66e-83 | 244 |
| MsaG024845 | MtrunA17_Chr1g0204971 | 59.375 | 192 | 74 | 2 | 1 | 190 | 1 | 190 | 4.91e-80 | 236 |
| MsaG024845 | MtrunA17_Chr7g0273661 | 41.667 | 156 | 88 | 1 | 17 | 172 | 4 | 156 | 2.59e-42 | 139 |
| MsaG024845 | MtrunA17_Chr7g0223361 | 39.080 | 174 | 96 | 3 | 17 | 187 | 4 | 170 | 2.65e-37 | 127 |
| MsaG024845 | MtrunA17_Chr7g0223351 | 39.286 | 168 | 97 | 3 | 20 | 187 | 7 | 169 | 5.08e-36 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG024845 | AT2G32030.1 | 51.190 | 168 | 75 | 3 | 21 | 187 | 24 | 185 | 2.54e-58 | 181 |
| MsaG024845 | AT2G32020.1 | 46.561 | 189 | 84 | 4 | 2 | 187 | 6 | 180 | 3.26e-54 | 171 |
| MsaG024845 | AT3G22560.1 | 40.237 | 169 | 96 | 2 | 21 | 187 | 7 | 172 | 3.12e-37 | 127 |
Find 38 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTAAGAATTCCTTCCTTC+TGG | 0.242611 | 5:+1402558 | None:intergenic |
| ACAGAACTTGCCTATGTTCT+AGG | 0.250597 | 5:-1402731 | MsaT024845.1:CDS |
| GTTGATGCCTTGTTCTTTGC+TGG | 0.260002 | 5:+1402874 | None:intergenic |
| ATGTTCTAGGATCTAAATAT+TGG | 0.302360 | 5:-1402718 | MsaT024845.1:CDS |
| CCTTGTTCTTTGCTGGTGTA+TGG | 0.318147 | 5:+1402881 | None:intergenic |
| TGTTCTAGGATCTAAATATT+GGG | 0.318462 | 5:-1402717 | MsaT024845.1:CDS |
| TGCCTTAATGATCGTGCTAT+TGG | 0.357294 | 5:-1402806 | MsaT024845.1:CDS |
| ATATACCAAACAAATTTCTA+TGG | 0.388477 | 5:-1402841 | MsaT024845.1:CDS |
| TCCAGAAGGAAGGAATTCTT+AGG | 0.397920 | 5:-1402559 | MsaT024845.1:CDS |
| GGCCAATAGCACGATCATTA+AGG | 0.403869 | 5:+1402804 | None:intergenic |
| TTGCACCATAGAAATTTGTT+TGG | 0.404833 | 5:+1402836 | None:intergenic |
| TTGTTGATGTTGAAAATGTT+GGG | 0.408191 | 5:-1402610 | MsaT024845.1:CDS |
| GAAAAGGTCGGAGTGCGATT+TGG | 0.410859 | 5:+1402975 | None:intergenic |
| CCAAACAAATAAATCATAGT+TGG | 0.419913 | 5:+1402471 | None:intergenic |
| TTCTGACCTTGATGAGCTCC+TGG | 0.433077 | 5:-1402948 | MsaT024845.1:CDS |
| TTTGTTGATGTTGAAAATGT+TGG | 0.442167 | 5:-1402611 | MsaT024845.1:CDS |
| AGTGGCCAAGTTCTGCTCTT+GGG | 0.462852 | 5:-1402906 | MsaT024845.1:CDS |
| AAGTGGCCAAGTTCTGCTCT+TGG | 0.474319 | 5:-1402907 | MsaT024845.1:CDS |
| GGATCTAAATATTGGGGTAA+AGG | 0.475846 | 5:-1402710 | MsaT024845.1:CDS |
| AGGAAGTATTTGTTCATGAA+AGG | 0.475944 | 5:-1402539 | MsaT024845.1:CDS |
| TTTCATCAGCTCTCCATACC+AGG | 0.488476 | 5:+1402930 | None:intergenic |
| CAAAGAGTGTTGGAGAAAGC+TGG | 0.497277 | 5:-1402584 | MsaT024845.1:CDS |
| TCAAGGTCAGAAAGATGAAA+AGG | 0.513299 | 5:+1402959 | None:intergenic |
| ATATTTAGATCCTAGAACAT+AGG | 0.519203 | 5:+1402721 | None:intergenic |
| ACCTTGATGAGCTCCTGGTA+TGG | 0.539184 | 5:-1402943 | MsaT024845.1:CDS |
| GATCACCTCATGATAAAAGC+AGG | 0.555612 | 5:-1402763 | MsaT024845.1:CDS |
| TCCATACCAGGAGCTCATCA+AGG | 0.558283 | 5:+1402942 | None:intergenic |
| TGTTGGGTCTCAAAGAGTGT+TGG | 0.566749 | 5:-1402594 | MsaT024845.1:CDS |
| TGGTTCCCAAGAGCAGAACT+TGG | 0.574822 | 5:+1402901 | None:intergenic |
| GTTCTAGGATCTAAATATTG+GGG | 0.601221 | 5:-1402716 | MsaT024845.1:CDS |
| CCATACACCAGCAAAGAACA+AGG | 0.601334 | 5:-1402881 | MsaT024845.1:CDS |
| GGAGAAAGCTGGTTTCCAGA+AGG | 0.617484 | 5:-1402573 | MsaT024845.1:CDS |
| AACTTGTTTCACAACATACG+TGG | 0.623050 | 5:+1402682 | None:intergenic |
| AAAGCTGGTTTCCAGAAGGA+AGG | 0.623258 | 5:-1402569 | MsaT024845.1:CDS |
| GGTCAGAAAGATGAAAAGGT+CGG | 0.638104 | 5:+1402963 | None:intergenic |
| TGTTGTGAAACAAGTTGTCA+AGG | 0.644645 | 5:-1402675 | MsaT024845.1:CDS |
| TTTATTTCTTCCAAACCAGG+TGG | 0.661410 | 5:-1403022 | MsaT024845.1:CDS |
| ATCTTGATTTCAGAGAAACA+CGG | 0.706701 | 5:+1402783 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 1402484 | 1403056 | 1402484 | ID=MsaG024845 |
| Chr5 | mRNA | 1402484 | 1403056 | 1402484 | ID=MsaT024845.1;Parent=MsaG024845 |
| Chr5 | exon | 1402484 | 1403056 | 1402484 | ID=MsaT024845.1.exon1;Parent=MsaT024845.1 |
| Chr5 | CDS | 1402484 | 1403056 | 1402484 | ID=cds.MsaT024845.1;Parent=MsaT024845.1 |
| Gene Sequence |
| Protein sequence |
