AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG025015

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025015 MtrunA17_Chr5g0396491 99.376 481 3 0 1 481 1 481 0.0 990
MsaG025015 MtrunA17_Chr4g0038851 75.783 479 108 6 4 481 1 472 0.0 742
MsaG025015 MtrunA17_Chr3g0085681 54.139 447 193 5 34 480 4 438 6.01e-170 486
MsaG025015 MtrunA17_Chr3g0085361 55.056 356 148 5 125 480 9 352 1.03e-133 390
MsaG025015 MtrunA17_Chr7g0224271 34.658 453 209 7 45 478 289 673 4.55e-83 270
MsaG025015 MtrunA17_Chr5g0447691 30.254 433 233 8 53 478 174 544 8.97e-60 205
MsaG025015 MtrunA17_Chr8g0357601 30.455 440 236 10 49 479 175 553 2.12e-59 204
MsaG025015 MtrunA17_Chr1g0159461 28.605 430 240 7 55 478 224 592 7.45e-54 190
MsaG025015 MtrunA17_Chr2g0306641 29.571 443 232 10 47 479 116 488 5.03e-53 186
MsaG025015 MtrunA17_Chr3g0125811 28.140 430 242 7 55 478 202 570 1.89e-52 186
MsaG025015 MtrunA17_Chr4g0031721 28.009 432 237 7 56 477 316 683 7.12e-47 172
MsaG025015 MtrunA17_Chr4g0031701 25.959 443 260 6 42 475 240 623 4.57e-46 169
MsaG025015 MtrunA17_Chr4g0057891 27.563 439 249 13 50 479 143 521 6.48e-46 167
MsaG025015 MtrunA17_Chr4g0031711 27.189 434 248 7 53 477 358 732 1.10e-45 169
MsaG025015 MtrunA17_Chr4g0031741 26.620 432 247 7 53 477 281 649 3.18e-45 167
MsaG025015 MtrunA17_Chr1g0207361 92.079 101 8 0 282 382 64 164 9.84e-45 154
MsaG025015 MtrunA17_Chr1g0207361 90.741 54 5 0 90 143 1 54 2.39e-27 107
MsaG025015 MtrunA17_Chr2g0290241 27.635 427 247 10 57 479 58 426 2.39e-43 158
MsaG025015 MtrunA17_Chr4g0031731 25.734 443 261 7 42 475 240 623 3.00e-43 161
MsaG025015 MtrunA17_Chr7g0238891 27.040 429 247 7 56 478 372 740 2.28e-42 160
MsaG025015 MtrunA17_Chr2g0328341 26.531 441 258 9 42 477 258 637 2.51e-42 159
MsaG025015 MtrunA17_Chr4g0076091 26.512 430 246 10 56 477 175 542 3.44e-42 157
MsaG025015 MtrunA17_Chr1g0183641 28.899 436 236 11 48 480 115 479 1.01e-41 155
MsaG025015 MtrunA17_Chr2g0328381 25.792 442 262 9 41 477 260 640 2.84e-41 156
MsaG025015 MtrunA17_Chr7g0245601 41.146 192 108 2 60 249 442 630 1.86e-39 152
MsaG025015 MtrunA17_Chr7g0245601 38.255 149 87 3 333 478 646 792 2.38e-23 103
MsaG025015 MtrunA17_Chr4g0039661 26.147 436 242 9 53 478 190 555 3.15e-38 146
MsaG025015 MtrunA17_Chr2g0294471 27.056 462 256 12 33 478 180 576 1.10e-37 145
MsaG025015 MtrunA17_Chr4g0057291 33.023 215 140 1 49 259 285 499 1.56e-37 145
MsaG025015 MtrunA17_Chr4g0057291 32.692 156 98 5 328 477 507 661 2.19e-19 91.3
MsaG025015 MtrunA17_Chr3g0110971 34.685 222 131 3 44 260 207 419 2.21e-36 142
MsaG025015 MtrunA17_Chr3g0110971 37.805 164 89 3 328 478 424 587 8.11e-30 122
MsaG025015 MtrunA17_Chr8g0376381 34.375 224 128 5 44 260 157 368 1.43e-33 133
MsaG025015 MtrunA17_Chr8g0376381 35.526 152 95 2 328 478 373 522 9.26e-29 119
MsaG025015 MtrunA17_Chr2g0328401 26.893 383 209 8 56 431 290 608 6.88e-33 132
MsaG025015 MtrunA17_Chr2g0319091 35.238 210 129 2 53 256 209 417 8.99e-32 128
MsaG025015 MtrunA17_Chr2g0319091 41.611 149 86 1 329 477 431 578 2.28e-29 121
MsaG025015 MtrunA17_Chr5g0445331 28.972 214 144 3 53 258 158 371 2.82e-29 120
MsaG025015 MtrunA17_Chr5g0445331 36.879 141 88 1 338 478 393 532 5.25e-24 105
MsaG025015 MtrunA17_Chr4g0038761 32.124 193 128 2 60 249 88 280 1.07e-28 118
MsaG025015 MtrunA17_Chr4g0038761 39.456 147 87 2 332 478 298 442 3.19e-21 96.3
MsaG025015 MtrunA17_Chr2g0328371 25.597 293 156 6 139 428 13 246 3.36e-28 112
MsaG025015 MtrunA17_Chr2g0328361 23.409 440 248 10 42 477 260 614 1.29e-27 116
MsaG025015 MtrunA17_Chr3g0101141 27.645 293 187 7 53 329 172 455 7.82e-27 113
MsaG025015 MtrunA17_Chr3g0101141 36.170 141 89 1 338 478 403 542 2.49e-25 108
MsaG025015 MtrunA17_Chr2g0328391 29.048 210 142 2 56 258 299 508 1.47e-26 113
MsaG025015 MtrunA17_Chr2g0328391 28.188 149 107 0 329 477 520 668 1.90e-18 88.6
MsaG025015 MtrunA17_Chr7g0270031 25.877 456 256 13 36 478 168 554 7.53e-26 110
MsaG025015 MtrunA17_Chr5g0401421 24.658 438 287 11 56 477 35 445 9.88e-26 109
MsaG025015 MtrunA17_Chr5g0424131 26.350 463 245 15 41 477 106 498 1.00e-25 110
MsaG025015 MtrunA17_Chr4g0053901 23.729 472 285 12 38 477 39 467 2.28e-25 108
MsaG025015 MtrunA17_Chr2g0328411 31.100 209 134 2 56 258 289 493 5.24e-25 108
MsaG025015 MtrunA17_Chr2g0328411 30.201 149 104 0 329 477 505 653 8.18e-18 86.7
MsaG025015 MtrunA17_Chr2g0328431 31.100 209 134 2 56 258 289 493 1.38e-24 107
MsaG025015 MtrunA17_Chr2g0328431 29.530 149 105 0 329 477 505 653 1.90e-16 82.0
MsaG025015 MtrunA17_Chr7g0236851 27.586 348 176 10 141 477 374 656 8.32e-24 105
MsaG025015 MtrunA17_Chr5g0401131 30.769 221 137 5 50 258 108 324 8.60e-24 103
MsaG025015 MtrunA17_Chr5g0401131 29.032 155 103 4 329 478 338 490 3.98e-11 65.1
MsaG025015 MtrunA17_Chr2g0294461 34.615 156 93 4 328 478 434 585 2.90e-22 100
MsaG025015 MtrunA17_Chr2g0294461 29.032 248 160 6 33 264 186 433 2.99e-20 94.0
MsaG025015 MtrunA17_Chr8g0383981 24.598 435 243 16 53 477 136 495 6.06e-22 98.6
MsaG025015 MtrunA17_Chr2g0329591 26.512 215 148 4 47 252 65 278 1.24e-20 94.4
MsaG025015 MtrunA17_Chr6g0468561 23.570 437 249 12 57 478 296 662 7.76e-20 92.8
MsaG025015 MtrunA17_Chr1g0200401 27.839 273 159 9 47 288 71 336 1.06e-19 91.7
MsaG025015 MtrunA17_Chr1g0200401 29.143 175 106 4 323 479 286 460 6.09e-19 89.4
MsaG025015 MtrunA17_Chr4g0012071 22.664 428 254 12 57 479 197 552 6.75e-19 89.7
MsaG025015 MtrunA17_Chr3g0114841 28.163 245 136 8 41 255 105 339 2.51e-18 87.8
MsaG025015 MtrunA17_Chr3g0114841 31.707 164 98 5 321 477 343 499 8.17e-14 73.6
MsaG025015 MtrunA17_Chr1g0194621 29.570 186 117 5 297 478 343 518 5.96e-18 86.7
MsaG025015 MtrunA17_Chr1g0194621 29.388 245 154 9 36 263 124 366 1.86e-13 72.4
MsaG025015 MtrunA17_Chr3g0087711 52.542 59 28 0 207 265 1 59 8.93e-14 65.9
MsaG025015 MtrunA17_Chr2g0323111 26.846 149 107 2 329 477 159 305 1.05e-13 72.0
MsaG025015 MtrunA17_Chr7g0230731 28.205 195 134 2 33 223 349 541 3.01e-13 72.4
MsaG025015 MtrunA17_Chr7g0230771 30.303 165 113 1 33 197 352 514 5.33e-13 71.6
MsaG025015 MtrunA17_Chr8g0344101 23.409 440 245 16 50 466 217 587 8.75e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025015 AT1G50420.1 65.914 487 155 7 1 481 1 482 0.0 650
MsaG025015 AT3G54220.1 33.258 442 218 10 45 480 282 652 1.11e-60 210
MsaG025015 AT1G50600.3 29.476 458 251 10 31 478 131 526 6.69e-55 192
MsaG025015 AT1G50600.1 29.476 458 251 10 31 478 202 597 2.24e-54 192
MsaG025015 AT1G50600.2 29.476 458 251 10 31 478 206 601 2.25e-54 192
MsaG025015 AT1G21450.1 29.002 431 238 5 55 478 224 593 2.89e-54 192
MsaG025015 AT4G17230.1 26.622 447 259 7 39 478 141 525 7.53e-47 170
MsaG025015 AT4G17230.2 26.622 447 259 7 39 478 141 525 7.53e-47 170
MsaG025015 AT1G07520.2 24.099 444 268 6 45 477 143 528 8.17e-44 162
MsaG025015 AT1G07520.4 24.099 444 268 6 45 477 143 528 8.17e-44 162
MsaG025015 AT1G07520.3 24.099 444 268 6 45 477 308 693 4.47e-43 162
MsaG025015 AT1G07520.1 24.099 444 268 6 45 477 307 692 4.61e-43 162
MsaG025015 AT3G46600.2 26.097 433 253 6 55 481 3 374 3.49e-41 151
MsaG025015 AT1G07530.1 25.408 429 253 7 56 477 396 764 1.78e-40 155
MsaG025015 AT5G59450.1 25.688 436 253 9 53 478 224 598 3.07e-40 153
MsaG025015 AT3G46600.3 26.097 433 253 6 55 481 179 550 3.80e-40 152
MsaG025015 AT3G46600.1 26.097 433 253 6 55 481 211 582 6.98e-40 152
MsaG025015 AT2G29065.1 25.513 439 247 10 56 477 252 627 8.57e-39 149
MsaG025015 AT1G66350.1 35.616 219 132 4 44 260 143 354 2.84e-36 140
MsaG025015 AT1G66350.1 37.179 156 93 2 327 481 358 509 4.47e-31 126
MsaG025015 AT1G14920.1 36.161 224 129 4 44 260 160 376 1.08e-34 136
MsaG025015 AT1G14920.1 34.868 152 95 2 328 478 381 529 9.32e-27 113
MsaG025015 AT2G01570.1 35.683 227 126 5 44 260 212 428 1.87e-34 136
MsaG025015 AT2G01570.1 37.500 152 91 3 328 478 433 581 5.01e-27 114
MsaG025015 AT5G17490.1 34.404 218 132 4 47 260 151 361 2.51e-34 135
MsaG025015 AT5G17490.1 38.562 153 92 2 327 478 365 516 2.14e-27 115
MsaG025015 AT5G48150.1 28.975 283 189 4 57 329 123 403 7.01e-34 134
MsaG025015 AT5G48150.1 36.709 158 99 1 321 478 334 490 9.63e-27 113
MsaG025015 AT5G48150.4 28.975 283 189 4 57 329 123 403 7.01e-34 134
MsaG025015 AT5G48150.4 36.709 158 99 1 321 478 334 490 9.63e-27 113
MsaG025015 AT5G48150.2 28.975 283 189 4 57 329 123 403 7.01e-34 134
MsaG025015 AT5G48150.2 36.709 158 99 1 321 478 334 490 9.63e-27 113
MsaG025015 AT5G48150.3 28.975 283 189 4 57 329 123 403 7.01e-34 134
MsaG025015 AT5G48150.3 36.709 158 99 1 321 478 334 490 9.63e-27 113
MsaG025015 AT5G66770.1 27.397 438 236 13 53 478 217 584 4.10e-33 132
MsaG025015 AT3G03450.1 32.287 223 142 4 44 260 171 390 1.25e-32 130
MsaG025015 AT3G03450.1 39.355 155 92 2 325 478 392 545 2.42e-31 127
MsaG025015 AT1G63100.1 32.110 218 139 3 48 259 244 458 2.32e-32 130
MsaG025015 AT1G63100.1 36.196 163 91 3 328 477 466 628 9.66e-25 108
MsaG025015 AT1G63100.2 32.110 218 139 3 48 259 269 483 2.89e-32 130
MsaG025015 AT1G63100.2 36.196 163 91 3 328 477 491 653 1.29e-24 107
MsaG025015 AT5G41920.1 32.338 201 132 3 53 249 40 240 1.55e-31 125
MsaG025015 AT5G41920.1 36.774 155 91 3 327 480 254 402 1.94e-20 93.6
MsaG025015 AT2G37650.1 24.186 430 256 7 56 478 347 713 3.67e-31 127
MsaG025015 AT2G37650.2 24.186 430 256 7 56 478 335 701 5.11e-31 127
MsaG025015 AT2G04890.1 33.491 212 136 3 56 267 53 259 9.64e-31 123
MsaG025015 AT2G04890.1 40.000 150 89 1 329 478 265 413 9.14e-28 115
MsaG025015 AT5G52510.1 25.397 441 233 11 61 478 273 640 2.88e-30 124
MsaG025015 AT1G55580.1 36.184 152 96 1 328 478 294 445 3.81e-28 116
MsaG025015 AT1G55580.1 24.375 320 197 7 52 329 40 356 7.76e-15 77.0
MsaG025015 AT3G49950.1 24.831 443 255 12 52 477 26 407 5.31e-25 107
MsaG025015 AT2G29060.1 29.245 212 141 3 56 259 321 531 3.42e-24 106
MsaG025015 AT2G29060.1 27.273 154 103 3 329 477 542 691 2.11e-15 79.3
MsaG025015 AT2G29060.2 29.245 212 141 3 56 259 284 494 3.87e-24 106
MsaG025015 AT2G29060.2 27.273 154 103 3 329 477 505 654 1.71e-15 79.7
MsaG025015 AT4G37650.1 26.432 227 135 4 56 258 146 364 1.72e-22 100
MsaG025015 AT4G37650.1 26.415 159 107 3 329 478 372 529 6.45e-11 65.1
MsaG025015 AT4G08250.1 29.596 223 139 7 47 255 98 316 3.62e-20 93.6
MsaG025015 AT3G50650.1 30.876 217 133 4 58 267 177 383 6.22e-20 93.2
MsaG025015 AT3G50650.1 28.859 149 99 3 337 478 394 542 1.80e-13 73.2

Find 92 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATTCTTGTGGAATTTA+AGG 0.089377 5:+3006680 None:intergenic
TATGCTTTCTGAAGAAATTA+TGG 0.230947 5:-3006658 MsaT025015.1:CDS
TTTGAGTTTCTTGACATAAT+AGG 0.254589 5:+3006210 None:intergenic
TGCAGCATTGTTTGATTGTT+TGG 0.259936 5:-3005920 MsaT025015.1:CDS
CAGCTTCTTCAGAAAGCTTA+TGG 0.264058 5:+3006400 None:intergenic
TCATCAGCAGAAAGAGGTTT+TGG 0.271986 5:-3006433 MsaT025015.1:CDS
AACATACCATAATAGCTTAA+AGG 0.277960 5:+3005730 None:intergenic
ACAATGCTGCATAAGAATAT+AGG 0.281076 5:+3005932 None:intergenic
TCAAGCTATTGTTGAATCTA+TGG 0.285430 5:-3006577 MsaT025015.1:CDS
TGCTAAGAACAGGGTTGAAT+TGG 0.312189 5:+3006358 None:intergenic
CTCAAATGAGGAGGACCTTC+AGG 0.312197 5:+3006468 None:intergenic
TTGTGGAATTTAAGGCTCTA+TGG 0.321651 5:+3006688 None:intergenic
ATCTATGGAAGGTGAGAAAA+TGG 0.335503 5:-3006562 MsaT025015.1:CDS
TCTGCAGTTACATTCCCTTT+TGG 0.339325 5:-3006268 MsaT025015.1:CDS
AACATCCCTAGAGAGATTAA+AGG 0.354098 5:-3005881 MsaT025015.1:CDS
AAGCTATTATGGTATGTTGC+AGG 0.357915 5:-3005725 MsaT025015.1:CDS
AATCACCTAATGATGTGTTT+TGG 0.364016 5:+3006154 None:intergenic
TAGTAACTTGCTGGCAAGAT+AGG 0.366004 5:-3005639 MsaT025015.1:CDS
CAATGCTGCATAAGAATATA+GGG 0.366018 5:+3005933 None:intergenic
GACAAACTTCGTGTGAAAAC+CGG 0.371883 5:-3006315 MsaT025015.1:CDS
AATGTGCCTTTAAGCTATTA+TGG 0.377946 5:-3005736 MsaT025015.1:CDS
AGACTTGATTCGTGTGGATT+TGG 0.381293 5:-3005760 MsaT025015.1:CDS
ATGAACCAAAACACATCATT+AGG 0.381360 5:-3006159 MsaT025015.1:CDS
TGTGCAAATCATGTAGCTTC+TGG 0.411195 5:-3006846 MsaT025015.1:CDS
TCACCTAGCATAGGAGGAAT+AGG 0.416008 5:-3006945 MsaT025015.1:CDS
TATAGAATGAGAGAAGAAAA+TGG 0.416830 5:-3005667 MsaT025015.1:CDS
CTGATAGGATACTTAAAACT+TGG 0.427400 5:-3006719 MsaT025015.1:CDS
AGAAGGTTTCTACAGAGTTA+TGG 0.432910 5:-3005700 MsaT025015.1:CDS
TCACTGAAGCACTTGCTGAT+AGG 0.433040 5:-3006734 MsaT025015.1:CDS
ATGGTTGTGTAGTAACTTGC+TGG 0.448692 5:-3005648 MsaT025015.1:CDS
TCGGTTACAACCATGACTTT+TGG 0.460163 5:+3006006 None:intergenic
TCACAACGGTTCAACATTGA+TGG 0.461983 5:-3005971 MsaT025015.1:CDS
TTGCACAAGTGATTAACAAA+TGG 0.462673 5:+3006862 None:intergenic
CAACAACAGCTTGGAGAGCT+AGG 0.464849 5:-3005606 MsaT025015.1:CDS
TCTCAACCTTTAATCTCTCT+AGG 0.466126 5:+3005875 None:intergenic
ATTCATCATCCAAGGCCAAA+AGG 0.470579 5:+3006253 None:intergenic
GATGATGTTGAATCGGGACT+TGG 0.470973 5:+3006099 None:intergenic
GTTGTGATTCGAGTCCTGTT+CGG 0.477667 5:+3005987 None:intergenic
GAATCTATGTTACAAGAAGA+AGG 0.480629 5:-3007020 MsaT025015.1:CDS
AGAATTGAAATCTGAGGAAA+GGG 0.483022 5:-3006898 MsaT025015.1:CDS
ATATATGCCACCTTCAAGAA+AGG 0.483743 5:+3006609 None:intergenic
ATTATGTCAAGAAACTCAAA+TGG 0.483850 5:-3006207 MsaT025015.1:CDS
CCTCCTCATTTGAGAATCAC+AGG 0.490720 5:-3006459 MsaT025015.1:CDS
CTCTGCATAGTATCTCCATC+AGG 0.491525 5:+3006771 None:intergenic
AAGGTTGAGAAGATGCTGTT+TGG 0.498109 5:-3005862 MsaT025015.1:CDS
AGAAAGCATAATAATTCTTG+TGG 0.500494 5:+3006671 None:intergenic
AGTCATGGTTGTAACCGAAC+AGG 0.503090 5:-3006001 MsaT025015.1:CDS
CGACGATGATGATGTTGAAT+CGG 0.503259 5:+3006092 None:intergenic
GCTAGGTGAAACTGACATCA+TGG 0.505670 5:+3006959 None:intergenic
GAGAATTGAAATCTGAGGAA+AGG 0.506938 5:-3006899 MsaT025015.1:CDS
TCTCTACTAAGCCATGGATA+AGG 0.507017 5:+3006918 None:intergenic
AGGATACTTAAAACTTGGCC+TGG 0.515401 5:-3006714 MsaT025015.1:CDS
CTACAGAGTTATGGTTGTGA+GGG 0.516522 5:-3005691 MsaT025015.1:CDS
TCACAGCTTGCAACTCCTGA+TGG 0.516747 5:-3006786 MsaT025015.1:CDS
TTCATCATCCAAGGCCAAAA+GGG 0.519127 5:+3006254 None:intergenic
TTCAATTCTCTACTAAGCCA+TGG 0.520245 5:+3006912 None:intergenic
CTCAACCTTTAATCTCTCTA+GGG 0.522051 5:+3005876 None:intergenic
TATATGCCACCTTCAAGAAA+GGG 0.525767 5:+3006610 None:intergenic
TTGTGAAGGAGCTGAAAGAA+AGG 0.532875 5:-3005815 MsaT025015.1:CDS
AAAAGAGCAATCCATTGTGC+AGG 0.535750 5:+3006510 None:intergenic
AGGGATTCATCAGCAGAAAG+AGG 0.542783 5:-3006439 MsaT025015.1:CDS
TTCATCTGCTTCAATGAATG+TGG 0.545313 5:-3006061 MsaT025015.1:CDS
TTTAAGGCTCTATGGAGTCC+AGG 0.546774 5:+3006696 None:intergenic
ACTAATTGCAAGCGCCTCGC+CGG 0.552940 5:+3006296 None:intergenic
GACGATGATGATGTTGAATC+GGG 0.555408 5:+3006093 None:intergenic
ATGGTATGTTGCAGGCAAGA+AGG 0.557823 5:-3005717 MsaT025015.1:CDS
GATCCTATTCCTCCTATGCT+AGG 0.562234 5:+3006942 None:intergenic
TCAGTTTCACCTAGCATAGG+AGG 0.562988 5:-3006951 MsaT025015.1:CDS
AGAACAGGGTTGAATTGGAA+TGG 0.565131 5:+3006363 None:intergenic
TCTACAGAGTTATGGTTGTG+AGG 0.567967 5:-3005692 MsaT025015.1:CDS
ATTGAAGCAGATGAAGATGC+TGG 0.572949 5:+3006069 None:intergenic
GAGTTTGTCACCAAAAGTCA+TGG 0.575658 5:-3006016 MsaT025015.1:CDS
AAGAACATAATTGCTTGTGA+AGG 0.580873 5:-3005829 MsaT025015.1:CDS
ATCCCTGTGATTCTCAAATG+AGG 0.584689 5:+3006456 None:intergenic
TGAGGATGATTCATCATCCA+AGG 0.589137 5:+3006245 None:intergenic
AATCTATGTTACAAGAAGAA+GGG 0.598643 5:-3007019 MsaT025015.1:CDS
GAATAGGATCACCTTATCCA+TGG 0.599511 5:-3006929 MsaT025015.1:CDS
GCTATTGTTGAATCTATGGA+AGG 0.607832 5:-3006573 MsaT025015.1:CDS
TGAGAAGATGCTGTTTGGTG+AGG 0.611876 5:-3005857 MsaT025015.1:CDS
ATGTCAGTTTCACCTAGCAT+AGG 0.614550 5:-3006954 MsaT025015.1:CDS
ATCATGGAAAATAACTGTAG+AGG 0.617197 5:+3006975 None:intergenic
CTCCTCATTTGAGAATCACA+GGG 0.617281 5:-3006458 MsaT025015.1:CDS
TTTCTAGCTTGCTAAGAACA+GGG 0.622441 5:+3006349 None:intergenic
ACGGTTCAACATTGATGGAG+AGG 0.639461 5:-3005966 MsaT025015.1:CDS
TAGTAGAGAATTGAAATCTG+AGG 0.644490 5:-3006904 MsaT025015.1:CDS
TGGATGATGAATCATCCTCA+AGG 0.648643 5:-3006242 MsaT025015.1:CDS
ATGCTGCTGAACCTGCACAA+TGG 0.650823 5:-3006521 MsaT025015.1:CDS
GAATTGAAATCTGAGGAAAG+GGG 0.651416 5:-3006897 MsaT025015.1:CDS
TTTATGAGACTTGATTCGTG+TGG 0.658284 5:-3005766 MsaT025015.1:CDS
CCTGTGATTCTCAAATGAGG+AGG 0.663636 5:+3006459 None:intergenic
ACTTCGTGTGAAAACCGGCG+AGG 0.733150 5:-3006310 MsaT025015.1:CDS
GAACAGGACTCGAATCACAA+CGG 0.767714 5:-3005985 MsaT025015.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 3005600 3007045 3005600 ID=MsaG025015
Chr5 mRNA 3005600 3007045 3005600 ID=MsaT025015.1;Parent=MsaG025015
Chr5 exon 3005600 3007045 3005600 ID=MsaT025015.1.exon1;Parent=MsaT025015.1
Chr5 CDS 3005600 3007045 3005600 ID=cds.MsaT025015.1;Parent=MsaT025015.1
Gene Sequence

>MsaT025015.1

ATGGAATCTATGTTACAAGAAGAAGGGTCTTCATCAGTAACTTGTTCACCTCTACAGTTATTTTCCATGATGTCAGTTTCACCTAGCATAGGAGGAATAGGATCACCTTATCCATGGCTTAGTAGAGAATTGAAATCTGAGGAAAGGGGTTTGTATTTGATCCATTTGTTAATCACTTGTGCAAATCATGTAGCTTCTGGTAGTCTTGAAAGTGCAAACACAACACTTGAACAGATTTCACAGCTTGCAACTCCTGATGGAGATACTATGCAGAGAATTGCTGCTTATTTCACTGAAGCACTTGCTGATAGGATACTTAAAACTTGGCCTGGACTCCATAGAGCCTTAAATTCCACAAGAATTATTATGCTTTCTGAAGAAATTATGGTTCAGAAGTTTTTCTTTGAGCTTTTCCCTTTCTTGAAGGTGGCATATATTTTGACAAATCAAGCTATTGTTGAATCTATGGAAGGTGAGAAAATGGTTCATATTATTGATCTTAATGCTGCTGAACCTGCACAATGGATTGCTCTTTTACAAGTTTTGAGTGCAAGACCTGAAGGTCCTCCTCATTTGAGAATCACAGGGATTCATCAGCAGAAAGAGGTTTTGGAGCAAATGGGCCATAAGCTTTCTGAAGAAGCTGAGAAATTAGATATACCATTCCAATTCAACCCTGTTCTTAGCAAGCTAGAAAATCTTGATTTCGACAAACTTCGTGTGAAAACCGGCGAGGCGCTTGCAATTAGTTCTATTCTGCAGTTACATTCCCTTTTGGCCTTGGATGATGAATCATCCTCAAGGAGAAAAACTCCTATTATGTCAAGAAACTCAAATGGACTTCACCTGCAAAAAGCCATGCTTATGAACCAAAACACATCATTAGGTGATTTACTTGAAAAAGATATGGTTAATGGCTATAGTCCAAGTCCCGATTCAACATCATCATCGTCGCCAGCATCTTCATCTGCTTCAATGAATGTGGAGAGTTTTCTCAATGCTTTATGGAGTTTGTCACCAAAAGTCATGGTTGTAACCGAACAGGACTCGAATCACAACGGTTCAACATTGATGGAGAGGCTGCTTGAAGCCCTATATTCTTATGCAGCATTGTTTGATTGTTTGGAGTCAACTGTATCAAGAACATCCCTAGAGAGATTAAAGGTTGAGAAGATGCTGTTTGGTGAGGAAATCAAGAACATAATTGCTTGTGAAGGAGCTGAAAGAAAGGAAAGACATGAAAAACTTGATAAATGGTTTATGAGACTTGATTCGTGTGGATTTGGTAATGTGCCTTTAAGCTATTATGGTATGTTGCAGGCAAGAAGGTTTCTACAGAGTTATGGTTGTGAGGGATATAGAATGAGAGAAGAAAATGGTTGTGTAGTAACTTGCTGGCAAGATAGGTCTTTGTTTTCAACAACAGCTTGGAGAGCTAGGAAGTAA
Protein sequence

>MsaT025015.1

MESMLQEEGSSSVTCSPLQLFSMMSVSPSIGGIGSPYPWLSRELKSEERGLYLIHLLITCANHVASGSLESANTTLEQISQLATPDGDTMQRIAAYFTEALADRILKTWPGLHRALNSTRIIMLSEEIMVQKFFFELFPFLKVAYILTNQAIVESMEGEKMVHIIDLNAAEPAQWIALLQVLSARPEGPPHLRITGIHQQKEVLEQMGHKLSEEAEKLDIPFQFNPVLSKLENLDFDKLRVKTGEALAISSILQLHSLLALDDESSSRRKTPIMSRNSNGLHLQKAMLMNQNTSLGDLLEKDMVNGYSPSPDSTSSSSPASSSASMNVESFLNALWSLSPKVMVVTEQDSNHNGSTLMERLLEALYSYAALFDCLESTVSRTSLERLKVEKMLFGEEIKNIIACEGAERKERHEKLDKWFMRLDSCGFGNVPLSYYGMLQARRFLQSYGCEGYRMREENGCVVTCWQDRSLFSTTAWRARK*