Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG025043 | PNY03639.1 | 65.217 | 897 | 187 | 19 | 1 | 791 | 1 | 878 | 0.0 | 1085 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG025043 | sp|P25848|PHY1_CERPU | 36.220 | 127 | 71 | 4 | 236 | 355 | 1039 | 1162 | 1.45e-11 | 72.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG025043 | tr|A0A2K3NKW9|A0A2K3NKW9_TRIPR | 65.217 | 897 | 187 | 19 | 1 | 791 | 1 | 878 | 0.0 | 1085 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG025043 | MtrunA17_Chr5g0396901 | 89.019 | 428 | 3 | 1 | 1 | 384 | 25 | 452 | 0.0 | 791 |
| MsaG025043 | MtrunA17_Chr5g0396901 | 95.699 | 372 | 16 | 0 | 420 | 791 | 546 | 917 | 0.0 | 694 |
| MsaG025043 | MtrunA17_Chr8g0347481 | 40.893 | 582 | 244 | 11 | 1 | 493 | 1 | 571 | 9.94e-133 | 408 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG025043 | AT1G64300.2 | 43.664 | 726 | 277 | 17 | 1 | 621 | 1 | 699 | 0.0 | 542 |
| MsaG025043 | AT1G64300.1 | 43.664 | 726 | 277 | 17 | 1 | 621 | 1 | 699 | 0.0 | 542 |
| MsaG025043 | AT5G41730.2 | 41.997 | 731 | 285 | 15 | 1 | 624 | 1 | 699 | 3.11e-180 | 534 |
| MsaG025043 | AT5G41730.1 | 41.997 | 731 | 285 | 15 | 1 | 624 | 1 | 699 | 3.11e-180 | 534 |
| MsaG025043 | AT1G01450.1 | 38.636 | 308 | 126 | 8 | 241 | 492 | 67 | 367 | 1.35e-49 | 182 |
Find 142 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTCCTGCTTTGATATTT+TGG | 0.080066 | 5:+3354040 | None:intergenic |
| CTCTCAGATTCTGAGACTTT+TGG | 0.146039 | 5:+3353202 | None:intergenic |
| ATCAAACTCACAAGATATTT+TGG | 0.164131 | 5:+3353994 | None:intergenic |
| TTGATTCCCTTTGTTAATAT+TGG | 0.196770 | 5:+3353174 | None:intergenic |
| CAAGCAAAAGTTGTTGGATT+TGG | 0.204645 | 5:-3354321 | MsaT025043.1:CDS |
| GAGATTTGGCAAACAATATT+TGG | 0.205668 | 5:-3354992 | MsaT025043.1:CDS |
| AATTGCTTGCAAATTTCTTT+TGG | 0.212048 | 5:+3354967 | None:intergenic |
| GCTTATAAAGTTGTTGAAAA+AGG | 0.240086 | 5:-3353723 | MsaT025043.1:CDS |
| GTGTCATCAGAAATTGATTT+TGG | 0.241755 | 5:+3353528 | None:intergenic |
| GTAGGATATTACAAGGATTA+AGG | 0.243595 | 5:+3354376 | None:intergenic |
| TATTCCTACTATAGATAATA+AGG | 0.252638 | 5:-3353667 | MsaT025043.1:CDS |
| AAGAGATTAACCCTATAATT+TGG | 0.257480 | 5:-3354227 | MsaT025043.1:CDS |
| GTCCTCTTAGAGAGCTTTCC+AGG | 0.263161 | 5:-3355275 | MsaT025043.1:CDS |
| AACTGACCTTGGTCTTTCTT+TGG | 0.265277 | 5:+3353438 | None:intergenic |
| GGTTACCAGATCTTTAATTA+TGG | 0.266121 | 5:+3353554 | None:intergenic |
| CATAGATATTGCAATATGTT+TGG | 0.282076 | 5:+3354594 | None:intergenic |
| TCAGGTGCATACCAAATTAT+AGG | 0.292831 | 5:+3354216 | None:intergenic |
| TTTGTCTTTAACCTGTTAAA+TGG | 0.297516 | 5:+3353049 | None:intergenic |
| CGGCACATTGGTGAGGTTCT+TGG | 0.303933 | 5:-3355462 | MsaT025043.1:CDS |
| TATGGTCATGTCTCAGATTA+AGG | 0.304997 | 5:-3352722 | None:intergenic |
| TGGAAGTCTAAAAGCGCTTA+TGG | 0.306735 | 5:-3355442 | MsaT025043.1:CDS |
| TGAAACATGGCAGCATCTTC+TGG | 0.314429 | 5:+3352892 | None:intergenic |
| AAAAGAAATTTGCAAGCAAT+TGG | 0.319977 | 5:-3354965 | MsaT025043.1:CDS |
| GGATTGTCATCTGTTGAAAA+TGG | 0.333696 | 5:-3354300 | MsaT025043.1:CDS |
| ATTCTTTGAAGAAGTGAGTT+TGG | 0.334226 | 5:+3354879 | None:intergenic |
| GTCCTTTGATTGCTCACATA+TGG | 0.339488 | 5:+3352806 | None:intergenic |
| AGCATTGTGAGGATGCAAAA+AGG | 0.341134 | 5:-3352956 | MsaT025043.1:CDS |
| CATTATCTGGTCGGGTTTCA+AGG | 0.346406 | 5:-3353288 | MsaT025043.1:CDS |
| ATTATACATTAAGCATTGTT+TGG | 0.350263 | 5:-3355235 | MsaT025043.1:CDS |
| TTGACACGTCTTATTCTTGT+TGG | 0.355333 | 5:+3352992 | None:intergenic |
| GAAATTGATGAATGGTAATT+CGG | 0.363355 | 5:-3354827 | MsaT025043.1:CDS |
| TCGGGTTTCAAGGACAAATA+AGG | 0.364030 | 5:-3353278 | MsaT025043.1:CDS |
| GAGACCAATCACCATTTAAC+AGG | 0.371640 | 5:-3353060 | MsaT025043.1:CDS |
| ATTGTTTGGATTCAAAAGAT+TGG | 0.378122 | 5:-3355221 | MsaT025043.1:CDS |
| GTGAGAAAGTGATAACAAAT+TGG | 0.378799 | 5:+3354617 | None:intergenic |
| TTCTTTGGCTTCATTTGTGA+TGG | 0.379395 | 5:+3353453 | None:intergenic |
| TTAACCTGTTAAATGGTGAT+TGG | 0.382051 | 5:+3353056 | None:intergenic |
| ATAGATATTGCAATATGTTT+GGG | 0.387928 | 5:+3354595 | None:intergenic |
| AGTTCAAGCTGATTTAGTTC+AGG | 0.389760 | 5:+3353858 | None:intergenic |
| ATCTCATGCTTCAAATGGCC+AGG | 0.399868 | 5:-3354440 | MsaT025043.1:CDS |
| TTGAAGACAGAACTACAAAA+TGG | 0.399911 | 5:-3355311 | MsaT025043.1:CDS |
| ATTGTAACATTCTTCTTTGA+CGG | 0.401100 | 5:+3353483 | None:intergenic |
| TTGGATTCAAAAGATTGGTC+TGG | 0.401687 | 5:-3355216 | MsaT025043.1:CDS |
| GGGTTAATCTCTTCACCAAT+TGG | 0.403263 | 5:+3354237 | None:intergenic |
| CCATATGTTGTTCTTACATA+AGG | 0.409101 | 5:+3352740 | None:intergenic |
| ACAGTAGCCACATCCTTTGT+TGG | 0.413590 | 5:+3353630 | None:intergenic |
| TCATTACCACCAACATTATC+TGG | 0.424357 | 5:-3353301 | MsaT025043.1:CDS |
| ATGCTGCCATGTTTCAGATA+TGG | 0.424537 | 5:-3352885 | MsaT025043.1:CDS |
| GAGAATGCTGGGGAGATGTC+AGG | 0.427765 | 5:-3355102 | MsaT025043.1:CDS |
| TGGATTCAAAAGATTGGTCT+GGG | 0.432597 | 5:-3355215 | MsaT025043.1:CDS |
| TTATAAGCAAACATTTCATA+AGG | 0.434328 | 5:+3353738 | None:intergenic |
| CATCTTCACAAACTGTGCTT+TGG | 0.436780 | 5:+3353586 | None:intergenic |
| TGCATCCATAATTAAAGATC+TGG | 0.439192 | 5:-3353559 | MsaT025043.1:CDS |
| TTGTAACATTCTTCTTTGAC+GGG | 0.441892 | 5:+3353484 | None:intergenic |
| CTCAAAGGGCTCAATGCTAA+TGG | 0.444928 | 5:+3352764 | None:intergenic |
| CTCAAAGGACTCAATGGAAA+TGG | 0.446507 | 5:+3352830 | None:intergenic |
| ATTCCTACTATAGATAATAA+GGG | 0.450157 | 5:-3353666 | MsaT025043.1:CDS |
| TGAAAATGATTATCCTCAAA+AGG | 0.452553 | 5:+3354078 | None:intergenic |
| GCAAGCAATTGGAGAATGCT+TGG | 0.459665 | 5:-3354954 | MsaT025043.1:CDS |
| GAGAACAAAATCTGTCTCCT+TGG | 0.464084 | 5:+3354480 | None:intergenic |
| GGAGATGTCAGGGTTGGATC+AGG | 0.464317 | 5:-3355091 | MsaT025043.1:CDS |
| ACCACCAACATTATCTGGTC+GGG | 0.478543 | 5:-3353296 | MsaT025043.1:CDS |
| CACCGCCAATGAGTCCTTCA+AGG | 0.481111 | 5:-3352922 | MsaT025043.1:CDS |
| CAGGTGCATACCAAATTATA+GGG | 0.486379 | 5:+3354217 | None:intergenic |
| TCACCAACACATAATCCAAT+TGG | 0.489553 | 5:-3354252 | MsaT025043.1:CDS |
| TTCACCTTCTTTGAATACCC+TGG | 0.491717 | 5:+3355257 | None:intergenic |
| TACAAGGATTAAGGTTTCCA+TGG | 0.498240 | 5:+3354385 | None:intergenic |
| TATGGACTCAAAGGACTCAA+TGG | 0.498751 | 5:+3352824 | None:intergenic |
| CTACCCTTATTATCTATAGT+AGG | 0.503856 | 5:+3353663 | None:intergenic |
| ACCAACCAAAATATCAAAGC+AGG | 0.507591 | 5:-3354045 | MsaT025043.1:CDS |
| GTTCTTACATAAGGACTCAA+AGG | 0.508350 | 5:+3352749 | None:intergenic |
| ATTGAAGCGATCGAGAATGC+TGG | 0.508806 | 5:-3355114 | MsaT025043.1:CDS |
| CTTTCCAGGGTATTCAAAGA+AGG | 0.511780 | 5:-3355261 | MsaT025043.1:CDS |
| TTGAAGCGATCGAGAATGCT+GGG | 0.515187 | 5:-3355113 | MsaT025043.1:CDS |
| CTTCTGGTGTATGACCTTGA+AGG | 0.519264 | 5:+3352908 | None:intergenic |
| AGAATGCTTGGAGAGAAGAT+AGG | 0.519476 | 5:-3354942 | MsaT025043.1:CDS |
| AGTTGATGAACAAGGATTTG+TGG | 0.524686 | 5:-3354527 | MsaT025043.1:CDS |
| TGCTGGGGAGATGTCAGGGT+TGG | 0.526191 | 5:-3355097 | MsaT025043.1:CDS |
| GGAAATCCAATATTAACAAA+GGG | 0.528711 | 5:-3353180 | MsaT025043.1:CDS |
| CCTTATGTAAGAACAACATA+TGG | 0.529072 | 5:-3352740 | MsaT025043.1:CDS |
| AGGAAATCCAATATTAACAA+AGG | 0.532942 | 5:-3353181 | MsaT025043.1:CDS |
| TATGACCTTGAAGGACTCAT+TGG | 0.538589 | 5:+3352917 | None:intergenic |
| CAAGAAAATATGATGTGGAA+TGG | 0.539842 | 5:-3355038 | MsaT025043.1:CDS |
| ACACAATCGCAGATGAGATA+AGG | 0.544064 | 5:-3355347 | MsaT025043.1:CDS |
| CCATAACCCCAGTTAAAAGA+AGG | 0.545666 | 5:-3353228 | MsaT025043.1:CDS |
| GTGCCGGAATGGAACAGCTC+CGG | 0.546196 | 5:-3355482 | None:intergenic |
| TTTGTTCTCTATACCTGTGG+TGG | 0.547282 | 5:-3354466 | MsaT025043.1:CDS |
| GTAGATTGTTGTGACATTGA+AGG | 0.548073 | 5:-3353822 | MsaT025043.1:CDS |
| ATATTATATGTCCTCTCCAT+AGG | 0.548736 | 5:+3353780 | None:intergenic |
| ATGCTTGGAGAGAAGATAGG+TGG | 0.548742 | 5:-3354939 | MsaT025043.1:CDS |
| TATAATTTGGTATGCACCTG+AGG | 0.550991 | 5:-3354214 | MsaT025043.1:intron |
| TTCTTACATAAGGACTCAAA+GGG | 0.551714 | 5:+3352750 | None:intergenic |
| GGTTGATCTCATGCTTCAAA+TGG | 0.555778 | 5:-3354445 | MsaT025043.1:CDS |
| AACATATTGCAATATCTATG+TGG | 0.560041 | 5:-3354591 | MsaT025043.1:CDS |
| ACCCTGGAAAGCTCTCTAAG+AGG | 0.560256 | 5:+3355273 | None:intergenic |
| ATGGAACAGCTCCGGCACAT+TGG | 0.561500 | 5:-3355474 | MsaT025043.1:CDS |
| AGCAATCAAAGGACTAGAAG+AGG | 0.562315 | 5:-3352797 | MsaT025043.1:CDS |
| GGCGAGCGATATGGAAACAA+AGG | 0.564210 | 5:+3354015 | None:intergenic |
| CAAGAACCTCACCAATGTGC+CGG | 0.564531 | 5:+3355463 | None:intergenic |
| GTGGAGATATTCAATGCCCC+TGG | 0.567677 | 5:+3354422 | None:intergenic |
| TCAATGTCACAACAATCTAC+AGG | 0.568956 | 5:+3353825 | None:intergenic |
| GACCTTGAAGGACTCATTGG+CGG | 0.570717 | 5:+3352920 | None:intergenic |
| TTGTTGAAGAAATTGATGAA+TGG | 0.570718 | 5:-3354835 | MsaT025043.1:CDS |
| TCACCAATTGGATTATGTGT+TGG | 0.571772 | 5:+3354249 | None:intergenic |
| TACCACCAACATTATCTGGT+CGG | 0.572730 | 5:-3353297 | MsaT025043.1:CDS |
| GTCTGAGCCAACAAAGGATG+TGG | 0.574953 | 5:-3353637 | MsaT025043.1:CDS |
| TTGCTCACATATGGACTCAA+AGG | 0.582014 | 5:+3352815 | None:intergenic |
| TCTCATGCTTCAAATGGCCA+GGG | 0.583448 | 5:-3354439 | MsaT025043.1:CDS |
| TCGATCGCTTCAATAACCGC+AGG | 0.587690 | 5:+3355123 | None:intergenic |
| GAGGCTCATAGAAGCACTCA+AGG | 0.595114 | 5:-3354917 | MsaT025043.1:CDS |
| GTCCATATGTGAGCAATCAA+AGG | 0.596259 | 5:-3352808 | MsaT025043.1:CDS |
| AAAGCTAGAAACTCACAAGA+AGG | 0.600879 | 5:-3354351 | MsaT025043.1:CDS |
| AGAATGCTGGGGAGATGTCA+GGG | 0.601811 | 5:-3355101 | MsaT025043.1:CDS |
| TGAGAGAGAATTGTGGTCCA+AGG | 0.604253 | 5:-3354497 | MsaT025043.1:CDS |
| TCCTCTTAGAGAGCTTTCCA+GGG | 0.606681 | 5:-3355274 | MsaT025043.1:CDS |
| AACTTGATGTCAATGATGAC+TGG | 0.610334 | 5:+3353251 | None:intergenic |
| ACCCGACCAGATAATGTTGG+TGG | 0.618092 | 5:+3353295 | None:intergenic |
| AGCATGAGATCAACCACCAC+AGG | 0.618436 | 5:+3354453 | None:intergenic |
| CTTGGAGAGAAGATAGGTGG+AGG | 0.618932 | 5:-3354936 | MsaT025043.1:CDS |
| AAAATATCAAAGCAGGAGAG+AGG | 0.620693 | 5:-3354038 | MsaT025043.1:CDS |
| GAAACCCGACCAGATAATGT+TGG | 0.622199 | 5:+3353292 | None:intergenic |
| AACTATCCATATCTGAAACA+TGG | 0.625924 | 5:+3352879 | None:intergenic |
| ATGTCACAACAATCTACAGG+TGG | 0.639302 | 5:+3353828 | None:intergenic |
| ATGCTAGAGTGAATATGTCG+TGG | 0.640538 | 5:+3355373 | None:intergenic |
| AGGATTGATCAAAACATACT+CGG | 0.640765 | 5:+3353878 | None:intergenic |
| CAAAAGTCTCAGAATCTGAG+AGG | 0.650694 | 5:-3353201 | MsaT025043.1:CDS |
| TCTCTAAGAGGACATTCAAG+AGG | 0.651164 | 5:+3355285 | None:intergenic |
| ACAGCTCCGGCACATTGGTG+AGG | 0.651948 | 5:-3355469 | MsaT025043.1:CDS |
| GCTTGCAAGAAAATATGATG+TGG | 0.653459 | 5:-3355043 | MsaT025043.1:CDS |
| TAGTGAGTCTGAGCCAACAA+AGG | 0.653550 | 5:-3353643 | MsaT025043.1:CDS |
| CGATCGCTTCAATAACCGCA+GGG | 0.654532 | 5:+3355124 | None:intergenic |
| AATTGGATTATGTGTTGGTG+AGG | 0.655784 | 5:+3354254 | None:intergenic |
| TGTGACGGAGTTGATGAACA+AGG | 0.656381 | 5:-3354535 | MsaT025043.1:CDS |
| CTCATGCTTCAAATGGCCAG+GGG | 0.668271 | 5:-3354438 | MsaT025043.1:CDS |
| ATGAAGCCAAAGAAAGACCA+AGG | 0.681190 | 5:-3353444 | MsaT025043.1:intron |
| TGTAACATTCTTCTTTGACG+GGG | 0.687811 | 5:+3353485 | None:intergenic |
| GTACTAACAACAGCATTGTG+AGG | 0.690491 | 5:-3352967 | MsaT025043.1:CDS |
| GATAGTTCTTTGAAGATACG+AGG | 0.692687 | 5:-3352863 | MsaT025043.1:CDS |
| TGTCACAACAATCTACAGGT+GGG | 0.697110 | 5:+3353829 | None:intergenic |
| TGAAGCGATCGAGAATGCTG+GGG | 0.704654 | 5:-3355112 | MsaT025043.1:CDS |
| TTTGCTTTGCTATTTCCCTG+CGG | 0.707457 | 5:-3355139 | MsaT025043.1:CDS |
| ACATACATGAGAGAGAATTG+TGG | 0.732943 | 5:-3354504 | MsaT025043.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 3352724 | 3355496 | 3352724 | ID=MsaG025043 |
| Chr5 | mRNA | 3352724 | 3355496 | 3352724 | ID=MsaT025043.1;Parent=MsaG025043 |
| Chr5 | exon | 3352724 | 3353349 | 3352724 | ID=MsaT025043.1.exon5;Parent=MsaT025043.1 |
| Chr5 | CDS | 3352724 | 3353349 | 3352724 | ID=cds.MsaT025043.1;Parent=MsaT025043.1 |
| Chr5 | exon | 3353445 | 3353938 | 3353445 | ID=MsaT025043.1.exon4;Parent=MsaT025043.1 |
| Chr5 | CDS | 3353445 | 3353938 | 3353445 | ID=cds.MsaT025043.1;Parent=MsaT025043.1 |
| Chr5 | exon | 3354005 | 3354108 | 3354005 | ID=MsaT025043.1.exon3;Parent=MsaT025043.1 |
| Chr5 | CDS | 3354005 | 3354108 | 3354005 | ID=cds.MsaT025043.1;Parent=MsaT025043.1 |
| Chr5 | exon | 3354215 | 3354664 | 3354215 | ID=MsaT025043.1.exon2;Parent=MsaT025043.1 |
| Chr5 | CDS | 3354215 | 3354664 | 3354215 | ID=cds.MsaT025043.1;Parent=MsaT025043.1 |
| Chr5 | exon | 3354795 | 3355496 | 3354795 | ID=MsaT025043.1.exon1;Parent=MsaT025043.1 |
| Chr5 | CDS | 3354795 | 3355496 | 3354795 | ID=cds.MsaT025043.1;Parent=MsaT025043.1 |
| Gene Sequence |
| Protein sequence |