AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG025081


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025081 MtrunA17_Chr5g0397501 97.640 339 5 1 1 339 1 336 0.0 694
MsaG025081 MtrunA17_Chr1g0189211 58.108 74 27 1 23 92 145 218 2.62e-18 86.7
MsaG025081 MtrunA17_Chr2g0303101 52.703 74 31 1 23 92 47 120 3.31e-15 77.0
MsaG025081 MtrunA17_Chr8g0380981 50.000 74 33 1 23 92 38 111 5.19e-15 76.6
MsaG025081 MtrunA17_Chr7g0261401 46.512 86 40 2 28 107 117 202 5.60e-15 76.3
MsaG025081 MtrunA17_Chr3g0129871 50.943 53 26 0 240 292 15 67 9.83e-13 63.2
MsaG025081 MtrunA17_Chr1g0146731 35.443 79 49 1 245 321 26 104 3.62e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025081 AT1G63490.2 64.897 339 109 4 1 339 5 333 4.05e-147 451
MsaG025081 AT1G63490.4 64.897 339 109 4 1 339 5 333 4.11e-147 451
MsaG025081 AT1G63490.1 74.684 79 20 0 260 338 1 79 3.45e-36 140
MsaG025081 AT1G30810.3 48.864 88 39 2 11 92 40 127 2.20e-17 84.0
MsaG025081 AT1G30810.2 48.864 88 39 2 11 92 40 127 2.20e-17 84.0
MsaG025081 AT1G30810.1 48.864 88 39 2 11 92 40 127 2.20e-17 84.0
MsaG025081 AT2G34880.1 54.054 74 30 1 23 92 56 129 4.53e-17 83.2
MsaG025081 AT4G20400.1 48.649 74 34 1 23 92 51 124 4.10e-15 77.4
MsaG025081 AT3G01460.1 35.455 110 61 3 228 336 1269 1369 1.19e-13 73.2
MsaG025081 AT2G38950.1 55.102 49 22 0 24 72 104 152 3.04e-11 65.1
MsaG025081 AT5G24330.1 50.000 46 23 0 247 292 34 79 6.55e-11 63.5
MsaG025081 AT1G08620.2 54.054 74 30 1 23 92 141 214 8.29e-11 63.9
MsaG025081 AT1G08620.4 54.054 74 30 1 23 92 141 214 8.29e-11 63.9
MsaG025081 AT1G08620.1 54.054 74 30 1 23 92 141 214 8.29e-11 63.9
MsaG025081 AT1G08620.3 54.054 74 30 1 23 92 155 228 8.39e-11 63.9

Find 93 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAATTCCGTTGGGAAAT+TGG 0.191435 5:+3760655 MsaT025081.1:CDS
GGTATAGTTGGAGATGAAAT+TGG 0.198735 5:-3759871 None:intergenic
TGTTGATGATATGAAAATTA+AGG 0.230101 5:+3760485 MsaT025081.1:CDS
GAATTGTTCCACCGAATAAT+TGG 0.232815 5:+3759992 MsaT025081.1:CDS
ATTCCATAGGGTTCAGCTTC+AGG 0.265228 5:-3759964 None:intergenic
AACAACCCCATGATGCTTCT+TGG 0.283845 5:-3760466 None:intergenic
GATTTGTGTAAGGTGTTTAA+TGG 0.297746 5:+3760201 MsaT025081.1:CDS
TTTAATGGTGTTAAGAGGTT+TGG 0.341660 5:+3760216 MsaT025081.1:CDS
AAAGGAGGTTTCCAATTATT+CGG 0.343099 5:-3760003 None:intergenic
AAGACGTTTGAGCTGGAGTA+TGG 0.349029 5:+3760111 MsaT025081.1:CDS
TTGTGTGATAGGTGTGATAA+AGG 0.350074 5:+3760600 MsaT025081.1:CDS
TTTGATTTACTCTTAAATCT+TGG 0.365507 5:-3760834 None:intergenic
TGTAAGAGTAAGAGCGATCC+AGG 0.376652 5:+3760417 MsaT025081.1:CDS
AGGTTGTTGATGGGAAGAAA+TGG 0.380956 5:+3760250 MsaT025081.1:CDS
TCTATTTGCACCCTTGAAAC+AGG 0.385704 5:-3760801 None:intergenic
GGTTGTTGATGGGAAGAAAT+GGG 0.397367 5:+3760251 MsaT025081.1:CDS
TTGGACAGGGACCTGTTTCA+AGG 0.397459 5:+3760790 MsaT025081.1:CDS
TTGTTTCAATTGTTTGAGTT+CGG 0.399072 5:+3760680 MsaT025081.1:CDS
CAAGGTTTGTGAGGTGTAAT+GGG 0.402131 5:+3760283 MsaT025081.1:CDS
TCTTCAGTTGGATAATAAAC+AGG 0.414313 5:-3759901 None:intergenic
GGAGGATTTAGATTTGTGTA+AGG 0.419324 5:+3760191 MsaT025081.1:CDS
GAGGTTTGGTGGTTATGATA+AGG 0.422831 5:+3760230 MsaT025081.1:CDS
GGTTATGATAAGGTTGTTGA+TGG 0.423265 5:+3760240 MsaT025081.1:CDS
GTATAGTTGGAGATGAAATT+GGG 0.425199 5:-3759870 None:intergenic
CGATCCAGGGGTGGAGAGTT+CGG 0.426328 5:+3760431 MsaT025081.1:CDS
AGAGCATCTTTATGATTATG+AGG 0.428209 5:+3760350 MsaT025081.1:CDS
GCAAGGTTTGTGAGGTGTAA+TGG 0.433345 5:+3760282 MsaT025081.1:CDS
AGGTGATGCTATTGTGTGAT+AGG 0.443170 5:+3760589 MsaT025081.1:CDS
AGGTGTTTAATGGTGTTAAG+AGG 0.447558 5:+3760211 MsaT025081.1:CDS
GAGGTGTAATGGGAAGATTT+CGG 0.453749 5:+3760293 MsaT025081.1:CDS
AATGGTGTTAAGAGGTTTGG+TGG 0.457247 5:+3760219 MsaT025081.1:CDS
AGTTCGGATAGGGAGAGCTT+TGG 0.463981 5:+3760696 MsaT025081.1:CDS
AGGTCGAGGAGGAGATGGTT+TGG 0.465503 5:+3760771 MsaT025081.1:CDS
ACAGGTGCTGAAGGTATAGT+TGG 0.465671 5:-3759883 None:intergenic
GAGGGATTATTGTGGGAAGA+AGG 0.471748 5:+3760143 MsaT025081.1:CDS
CCCAACGGAATTTGCTTCAA+CGG 0.478420 5:-3760648 None:intergenic
TCCAGCGAATATTGCTTCCC+CGG 0.480546 5:-3760726 None:intergenic
CAATTGTTTGAGTTCGGATA+GGG 0.482205 5:+3760686 MsaT025081.1:CDS
GAATCTAGATCCAATGCAAA+AGG 0.495870 5:-3760021 None:intergenic
ACAGGCGATTCATAAGCTTC+AGG 0.496534 5:+3760065 MsaT025081.1:CDS
AATGTAAAAGTGGGTTGCAT+GGG 0.496716 5:+3760565 MsaT025081.1:CDS
ATCTTATGAATAAATTCTAA+AGG 0.499809 5:-3759937 None:intergenic
CGGTATCCAAGAAGCATCAT+GGG 0.500771 5:+3760460 MsaT025081.1:CDS
TCGGTATCCAAGAAGCATCA+TGG 0.501410 5:+3760459 MsaT025081.1:CDS
TGGACAGGGACCTGTTTCAA+GGG 0.502224 5:+3760791 MsaT025081.1:CDS
GGGAGGTTGCAAGGTTTGTG+AGG 0.502755 5:+3760274 MsaT025081.1:CDS
ATTTGTGAGCAATGTAAAAG+TGG 0.504631 5:+3760555 MsaT025081.1:CDS
GGTGGAGAGTTCGGTCAGTT+CGG 0.506687 5:+3760440 MsaT025081.1:CDS
GCCGTTGAAGCAAATTCCGT+TGG 0.507654 5:+3760647 MsaT025081.1:CDS
TCAATTGTTTGAGTTCGGAT+AGG 0.511648 5:+3760685 MsaT025081.1:CDS
AGACCTGAAGCTGAACCCTA+TGG 0.512319 5:+3759961 MsaT025081.1:CDS
CGTTTGAGCTGGAGTATGGT+AGG 0.512739 5:+3760115 MsaT025081.1:CDS
AGTAAAGGTACGAGCACGAG+TGG 0.514944 5:+3760390 MsaT025081.1:CDS
CAGATAGGTCGAGGAGGAGA+TGG 0.517677 5:+3760766 MsaT025081.1:CDS
CCGTTGAAGCAAATTCCGTT+GGG 0.524050 5:+3760648 MsaT025081.1:CDS
GAGGAGGAGATGGTTTGGAC+AGG 0.528649 5:+3760776 MsaT025081.1:CDS
TTGTTGATGGGAAGAAATGG+GGG 0.530821 5:+3760253 MsaT025081.1:CDS
AACAATTCTACAAATTCCAT+AGG 0.532031 5:-3759977 None:intergenic
AGGATTTGAAAGTGAAGGAT+CGG 0.532408 5:+3760505 MsaT025081.1:CDS
TGATTCGAAGACGTTTGAGC+TGG 0.533945 5:+3760104 MsaT025081.1:CDS
TCTTTGAATTCATCTTCAGT+TGG 0.535029 5:-3759913 None:intergenic
AGTGAAGGATCGGAAAGTAA+AGG 0.542799 5:+3760515 MsaT025081.1:CDS
GCCGGGGAAGCAATATTCGC+TGG 0.545338 5:+3760725 MsaT025081.1:CDS
CTGACCGAACTCTCCACCCC+TGG 0.545867 5:-3760435 None:intergenic
GACGCATTGCAGATAGGTCG+AGG 0.551708 5:+3760757 MsaT025081.1:CDS
TGTGTGATAGGTGTGATAAA+GGG 0.553046 5:+3760601 MsaT025081.1:CDS
AATTAAGGATTTGAAAGTGA+AGG 0.561224 5:+3760500 MsaT025081.1:CDS
GGATAATAAACAGGTGCTGA+AGG 0.564496 5:-3759892 None:intergenic
GTTGTTGATGGGAAGAAATG+GGG 0.565480 5:+3760252 MsaT025081.1:CDS
ACAATTCTACAAATTCCATA+GGG 0.569821 5:-3759976 None:intergenic
CAATGTAAAAGTGGGTTGCA+TGG 0.573025 5:+3760564 MsaT025081.1:CDS
GTCTTCGAATCACACGCAGC+AGG 0.578760 5:-3760093 None:intergenic
GGAGGTTTCCAATTATTCGG+TGG 0.582620 5:-3760000 None:intergenic
TGTTGATGGGAAGAAATGGG+GGG 0.588740 5:+3760254 MsaT025081.1:CDS
GTAAGAGTAAGAGCGATCCA+GGG 0.591679 5:+3760418 MsaT025081.1:CDS
GAGTAAGAGCGATCCAGGGG+TGG 0.597004 5:+3760422 MsaT025081.1:CDS
ATTGCTCACAAATCTGATCA+CGG 0.602209 5:-3760545 None:intergenic
GTTATGATAAGGTTGTTGAT+GGG 0.606122 5:+3760241 MsaT025081.1:CDS
AACGGAATTTGCTTCAACGG+CGG 0.606689 5:-3760645 None:intergenic
TAAAAGTGGGTTGCATGGGG+AGG 0.608073 5:+3760569 MsaT025081.1:CDS
TGATGGGGAAAGGAAACCCT+AGG 0.615366 5:+3759815 None:intergenic
GTAACAACAGTGATGGGGAA+AGG 0.616940 5:+3759805 None:intergenic
AAACAATACCAATTTCCCAA+CGG 0.622144 5:-3760663 None:intergenic
TCTAGATCCAATGCAAAAGG+AGG 0.631627 5:-3760018 None:intergenic
AGGAGGAGATGGTTTGGACA+GGG 0.639029 5:+3760777 MsaT025081.1:CDS
ATGTAAAAGTGGGTTGCATG+GGG 0.639965 5:+3760566 MsaT025081.1:CDS
TGAATCGCCTGTGTCTTCGT+CGG 0.640852 5:-3760054 None:intergenic
TAAGAGTAAGAGCGATCCAG+GGG 0.676576 5:+3760419 MsaT025081.1:CDS
GTAAAGGTACGAGCACGAGT+GGG 0.681686 5:+3760391 MsaT025081.1:CDS
TTTGTGAGCAATGTAAAAGT+GGG 0.690357 5:+3760556 MsaT025081.1:CDS
GCATTGCAGATAGGTCGAGG+AGG 0.691576 5:+3760760 MsaT025081.1:CDS
GTGATAGGTGTGATAAAGGG+TGG 0.717925 5:+3760604 MsaT025081.1:CDS
GGTATCCAAGAAGCATCATG+GGG 0.744312 5:+3760461 MsaT025081.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 3759817 3760893 3759817 ID=MsaG025081
Chr5 mRNA 3759817 3760893 3759817 ID=MsaT025081.1;Parent=MsaG025081
Chr5 exon 3759817 3760893 3759817 ID=MsaT025081.1.exon1;Parent=MsaT025081.1
Chr5 CDS 3759817 3760893 3759817 ID=cds.MsaT025081.1;Parent=MsaT025081.1
Gene Sequence

>MsaT025081.1

ATGGGGAAAGGAAACCCTAGGTCTGTAGAAAAACGTGTATTAGGTCAAAATCTCCCAATTTCATCTCCAACTATACCTTCAGCACCTGTTTATTATCCAACTGAAGATGAATTCAAAGATCCTTTAGAATTTATTCATAAGATTAGACCTGAAGCTGAACCCTATGGAATTTGTAGAATTGTTCCACCGAATAATTGGAAACCTCCTTTTGCATTGGATCTAGATTCTTTCACTTTTCCGACGAAGACACAGGCGATTCATAAGCTTCAGGTTCGTCCTGCTGCGTGTGATTCGAAGACGTTTGAGCTGGAGTATGGTAGGTTTTTGAGGGATTATTGTGGGAAGAAGGTTAAGAAGAGAGTTATTTTTGAAGGGGAGGATTTAGATTTGTGTAAGGTGTTTAATGGTGTTAAGAGGTTTGGTGGTTATGATAAGGTTGTTGATGGGAAGAAATGGGGGGAGGTTGCAAGGTTTGTGAGGTGTAATGGGAAGATTTCGGATTGTGCTAAGCATGTTTTGTGTCAGTTGTATAGAGAGCATCTTTATGATTATGAGGTTTTTTGTAATAAGTTTAGTAAAGGTACGAGCACGAGTGGGAGCTGTAAGAGTAAGAGCGATCCAGGGGTGGAGAGTTCGGTCAGTTCGGTATCCAAGAAGCATCATGGGGTTGTTGATGATATGAAAATTAAGGATTTGAAAGTGAAGGATCGGAAAGTAAAGGAAGAAAGCCGTGATCAGATTTGTGAGCAATGTAAAAGTGGGTTGCATGGGGAGGTGATGCTATTGTGTGATAGGTGTGATAAAGGGTGGCATATTTATTGTCTTTCACCGCCGTTGAAGCAAATTCCGTTGGGAAATTGGTATTGTTTCAATTGTTTGAGTTCGGATAGGGAGAGCTTTGGTTTTGTGCCGGGGAAGCAATATTCGCTGGAAACTTTTAGACGCATTGCAGATAGGTCGAGGAGGAGATGGTTTGGACAGGGACCTGTTTCAAGGGTGCAAATAGAGAAGAAATTGCCAAGATTTAAGAGTAAATCAAAATTACATTCATGTGATAATTGCAATGTAAAAGAGTAA

Protein sequence

>MsaT025081.1

MGKGNPRSVEKRVLGQNLPISSPTIPSAPVYYPTEDEFKDPLEFIHKIRPEAEPYGICRIVPPNNWKPPFALDLDSFTFPTKTQAIHKLQVRPAACDSKTFELEYGRFLRDYCGKKVKKRVIFEGEDLDLCKVFNGVKRFGGYDKVVDGKKWGEVARFVRCNGKISDCAKHVLCQLYREHLYDYEVFCNKFSKGTSTSGSCKSKSDPGVESSVSSVSKKHHGVVDDMKIKDLKVKDRKVKEESRDQICEQCKSGLHGEVMLLCDRCDKGWHIYCLSPPLKQIPLGNWYCFNCLSSDRESFGFVPGKQYSLETFRRIADRSRRRWFGQGPVSRVQIEKKLPRFKSKSKLHSCDNCNVKE*