AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG025251


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025251 MtrunA17_Chr5g0399491 59.848 132 19 1 9 106 56 187 8.25e-43 141
MsaG025251 MtrunA17_Chr4g0055851 48.120 133 30 6 9 106 43 171 4.53e-20 82.0
MsaG025251 MtrunA17_Chr8g0335131 37.594 133 34 4 23 106 65 197 5.19e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025251 AT4G17460.1 40.458 131 31 3 23 106 56 186 2.08e-16 72.8

Find 23 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATTGAAAAGGAAGATTT+TGG 0.211828 5:+5758139 MsaT025251.1:CDS
AAAGCAGACTGTTGGAAGTT+TGG 0.310732 5:+5758958 MsaT025251.1:CDS
CGATGAATCGAGTTACCTTC+TGG 0.337157 5:+5758571 MsaT025251.1:CDS
ATTGGCACTTGCAAAACAAT+TGG 0.337338 5:+5758929 MsaT025251.1:CDS
AAGAAGGAAAGAGAAGTTAC+AGG 0.389370 5:+5758661 MsaT025251.1:CDS
TTTACATGCAGATCGGGATT+CGG 0.433835 5:+5758505 MsaT025251.1:intron
ACACTTGAAACGGTGCTGTT+TGG 0.443597 5:-5758629 None:intergenic
TTGGCACTTGCAAAACAATT+GGG 0.452760 5:+5758930 MsaT025251.1:CDS
TTTACCACTCACACTTGAAA+CGG 0.484021 5:-5758639 None:intergenic
GATGAATCGAGTTACCTTCT+GGG 0.506572 5:+5758572 MsaT025251.1:CDS
GGGACTTCAAAGCAGACTGT+TGG 0.521714 5:+5758950 MsaT025251.1:CDS
AACTCGATTCATCGATTCCT+CGG 0.525093 5:-5758562 None:intergenic
TCGATTCCTCGGAGAAGTAA+AGG 0.525636 5:-5758551 None:intergenic
CAAGTGTGAGTGGTAAAAGA+AGG 0.534931 5:+5758645 MsaT025251.1:CDS
ATGGAAAGAGATTGTTCAAG+AGG 0.555724 5:+5758698 MsaT025251.1:CDS
GGTTTCAAAATCGTAGAGCA+AGG 0.583437 5:+5758979 MsaT025251.1:CDS
GCTGATTGTGAAGAAGAAGC+AGG 0.600333 5:+5758596 MsaT025251.1:CDS
TGGAAAGAGATTGTTCAAGA+GGG 0.616887 5:+5758699 MsaT025251.1:CDS
CTTCACAATCAGCACCCAGA+AGG 0.634945 5:-5758586 None:intergenic
AGCACCGTTTCAAGTGTGAG+TGG 0.674395 5:+5758635 MsaT025251.1:CDS
AGACATGCAGAATCGAAGAA+CGG 0.674956 5:+5758528 MsaT025251.1:CDS
GAACGGCCTTTACTTCTCCG+AGG 0.691005 5:+5758545 MsaT025251.1:CDS
ACAGGTAGAAGATCTTGACA+TGG 0.729649 5:+5758679 MsaT025251.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 5758136 5759004 5758136 ID=MsaG025251
Chr5 mRNA 5758136 5759004 5758136 ID=MsaT025251.1;Parent=MsaG025251
Chr5 exon 5758136 5758160 5758136 ID=MsaT025251.1.exon1;Parent=MsaT025251.1
Chr5 CDS 5758136 5758160 5758136 ID=cds.MsaT025251.1;Parent=MsaT025251.1
Chr5 exon 5758516 5758727 5758516 ID=MsaT025251.1.exon2;Parent=MsaT025251.1
Chr5 CDS 5758516 5758727 5758516 ID=cds.MsaT025251.1;Parent=MsaT025251.1
Chr5 exon 5758921 5759004 5758921 ID=MsaT025251.1.exon3;Parent=MsaT025251.1
Chr5 CDS 5758921 5759004 5758921 ID=cds.MsaT025251.1;Parent=MsaT025251.1
Gene Sequence

>MsaT025251.1

ATGAGTATTGAAAAGGAAGATTTTGATCGGGATTCGGAGACATGCAGAATCGAAGAACGGCCTTTACTTCTCCGAGGAATCGATGAATCGAGTTACCTTCTGGGTGCTGATTGTGAAGAAGAAGCAGGAGTTTCATCACCAAACAGCACCGTTTCAAGTGTGAGTGGTAAAAGAAGGAAAGAGAAGTTACAGGTAGAAGATCTTGACATGGAAAGAGATTGTTCAAGAGGGAAATCAAAACAAAAATTGGCACTTGCAAAACAATTGGGACTTCAAAGCAGACTGTTGGAAGTTTGGTTTCAAAATCGTAGAGCAAGGTTT

Protein sequence

>MsaT025251.1

MSIEKEDFDRDSETCRIEERPLLLRGIDESSYLLGADCEEEAGVSSPNSTVSSVSGKRRKEKLQVEDLDMERDCSRGKSKQKLALAKQLGLQSRLLEVWFQNRRARF