AlfalfaGEDB | MsaG025254

M. sativa ssp. caerulea / MsaG025254

KEGG: K09338
GO: GO:0000003 GO:0000902 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008283 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009641 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009735 GO:0009755 GO:0009791 GO:0009826 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009987 GO:0010016 GO:0010017 GO:0010022 GO:0010033 GO:0010073 GO:0010154 GO:0010218 GO:0010468 GO:0010556 GO:0010558 GO:0010582 GO:0010605 GO:0010629 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0040007 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043621 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048364 GO:0048367 GO:0048438 GO:0048467 GO:0048507 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070887 GO:0071214 GO:0071310 GO:0071365 GO:0071478 GO:0071482 GO:0071489 GO:0071495 GO:0071840 GO:0080090 GO:0080127 GO:0090567 GO:0097159 GO:0099402 GO:0104004 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1905328 GO:2000112 GO:2000113 GO:2001141
PFAM: PF04618.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025254	XP_003611347.1	98.311	296	5	0	1	296	1	296	0.0	598

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025254	sp\|Q05466\|HAT4_ARATH	65.101	298	88	7	1	296	1	284	5.54e-108	318

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025254	tr\|B7FJ62\|B7FJ62_MEDTR	98.311	296	5	0	1	296	1	296	0.0	598

TFs/TRs:

Gene ID	Type	Classification
MsaG025254	TF	HB-HD-ZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG003998	MsaG025254	0.804593	3.385504e-49	5.084255e-47
MsaG004534	MsaG025254	0.801120	1.751920e-48	2.429313e-46
MsaG006994	MsaG025254	0.850473	3.163836e-60	1.742811e-57
MsaG025254	MsaG040736	0.811834	9.856278e-51	1.760405e-48
MsaG025254	MsaG045689	0.811735	1.035435e-50	1.844848e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025254	MtrunA17_Chr5g0399491	98.311	296	5	0	1	296	1	296	0.0	598
MsaG025254	MtrunA17_Chr4g0055851	72.333	300	54	9	1	293	2	279	2.57e-138	392
MsaG025254	MtrunA17_Chr8g0335131	56.958	309	101	10	4	288	3	303	3.00e-94	281
MsaG025254	MtrunA17_Chr7g0258831	64.516	186	58	4	72	250	89	273	9.58e-68	213
MsaG025254	MtrunA17_Chr1g0175651	60.204	196	54	4	71	251	82	268	1.13e-66	210
MsaG025254	MtrunA17_Chr2g0308611	73.026	152	35	4	112	260	175	323	1.17e-65	209
MsaG025254	MtrunA17_Chr4g0054421	78.049	123	26	1	127	249	118	239	5.60e-61	194
MsaG025254	MtrunA17_Chr5g0400781	65.972	144	46	2	127	268	114	256	4.47e-60	192
MsaG025254	MtrunA17_Chr1g0151971	51.667	180	73	5	96	263	88	265	1.19e-51	171
MsaG025254	MtrunA17_Chr4g0044091	62.097	124	42	1	130	248	59	182	1.93e-45	152
MsaG025254	MtrunA17_Chr2g0299031	57.627	118	50	0	136	253	82	199	3.07e-41	142
MsaG025254	MtrunA17_Chr3g0135331	65.217	115	38	2	135	249	144	256	4.61e-39	139
MsaG025254	MtrunA17_Chr4g0071411	61.404	114	41	1	138	248	90	203	1.19e-38	135
MsaG025254	MtrunA17_Chr7g0217111	42.537	134	63	3	97	225	49	173	2.53e-20	88.2
MsaG025254	MtrunA17_Chr5g0415941	39.535	172	77	6	78	228	20	185	1.59e-19	87.0
MsaG025254	MtrunA17_Chr8g0362151	41.463	123	70	2	100	222	43	163	5.92e-19	84.7
MsaG025254	MtrunA17_Chr6g0450991	43.590	117	65	1	136	252	67	182	1.55e-18	83.6
MsaG025254	MtrunA17_Chr4g0060031	52.632	95	38	1	134	221	93	187	4.84e-18	83.2
MsaG025254	MtrunA17_Chr6g0453021	31.401	207	106	5	18	223	12	183	5.39e-18	82.0
MsaG025254	MtrunA17_Chr5g0416391	39.516	124	75	0	100	223	41	164	1.12e-17	81.3
MsaG025254	MtrunA17_Chr3g0119291	38.926	149	65	4	97	223	9	153	3.33e-17	79.3
MsaG025254	MtrunA17_Chr7g0265521	45.455	88	48	0	136	223	63	150	9.34e-17	77.8
MsaG025254	MtrunA17_Chr3g0123691	40.625	128	68	2	128	247	52	179	2.03e-16	77.8
MsaG025254	MtrunA17_Chr8g0381741	47.475	99	45	1	132	223	50	148	2.34e-16	77.8
MsaG025254	MtrunA17_Chr1g0179581	48.193	83	43	0	136	218	28	110	5.17e-16	72.8
MsaG025254	MtrunA17_Chr8g0362751	46.809	94	43	1	136	222	89	182	1.17e-15	75.5
MsaG025254	MtrunA17_Chr8g0346251	42.683	82	47	0	140	221	45	126	1.20e-11	63.5
MsaG025254	MtrunA17_Chr5g0410711	31.690	142	91	1	80	221	42	177	2.26e-11	64.3
MsaG025254	MtrunA17_Chr5g0403691	41.905	105	59	1	128	230	45	149	9.66e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025254	AT4G16780.1	65.101	298	88	7	1	296	1	284	5.63e-109	318
MsaG025254	AT5G47370.1	59.936	312	80	13	1	296	1	283	7.82e-107	312
MsaG025254	AT4G17460.1	59.032	310	85	11	1	296	1	282	2.93e-105	308
MsaG025254	AT3G60390.1	59.060	298	84	14	17	287	23	309	1.33e-91	275
MsaG025254	AT2G44910.1	53.165	316	115	12	4	288	3	316	3.19e-84	256
MsaG025254	AT5G06710.1	61.421	197	64	6	91	277	135	329	2.91e-67	213
MsaG025254	AT4G37790.1	59.358	187	57	5	105	273	86	271	3.56e-62	198
MsaG025254	AT2G22800.1	72.308	130	32	2	123	249	97	225	2.69e-58	188
MsaG025254	AT2G01430.1	52.299	174	65	4	92	256	95	259	5.51e-44	151
MsaG025254	AT1G70920.1	60.976	123	37	2	135	257	68	179	8.85e-38	133
MsaG025254	AT1G70920.2	60.976	123	37	2	135	257	38	149	9.10e-38	132
MsaG025254	AT5G06710.4	58.879	107	34	4	91	187	135	241	4.60e-27	107
MsaG025254	AT1G69780.1	41.221	131	67	2	98	223	47	172	4.93e-20	88.2
MsaG025254	AT2G01430.2	47.059	119	49	3	92	204	95	205	5.82e-19	84.0
MsaG025254	AT1G26960.1	46.341	123	61	4	103	223	39	158	1.78e-18	83.2
MsaG025254	AT3G01470.1	48.387	93	41	1	136	221	68	160	6.16e-17	79.3
MsaG025254	AT5G03790.1	37.313	134	83	1	136	269	77	209	4.44e-16	76.3
MsaG025254	AT5G15150.1	38.983	118	65	1	106	223	92	202	2.64e-15	75.5
MsaG025254	AT1G27045.4	47.748	111	45	3	127	224	53	163	3.64e-15	73.9
MsaG025254	AT3G01220.1	45.745	94	47	1	136	225	87	180	4.97e-15	74.3
MsaG025254	AT1G27045.1	54.321	81	31	1	127	201	53	133	9.36e-15	72.4
MsaG025254	AT1G27045.2	59.091	66	27	0	136	201	17	82	1.03e-14	71.2
MsaG025254	AT1G27045.5	59.091	66	27	0	136	201	25	90	1.08e-14	71.2
MsaG025254	AT1G27045.3	59.091	66	27	0	136	201	25	90	1.08e-14	71.2
MsaG025254	AT5G65310.1	35.417	144	84	3	133	269	69	210	1.87e-14	72.8
MsaG025254	AT4G40060.1	44.330	97	47	1	134	223	57	153	1.96e-14	72.8
MsaG025254	AT5G65310.2	35.417	144	84	3	133	269	51	192	2.40e-14	72.4
MsaG025254	AT2G22430.1	44.792	96	49	1	134	225	60	155	4.51e-14	71.6
MsaG025254	AT5G03790.2	43.956	91	51	0	136	226	77	167	3.94e-13	67.4
MsaG025254	AT2G46680.1	41.053	95	52	1	139	229	35	129	4.87e-13	68.2
MsaG025254	AT2G46680.2	40.909	88	52	0	139	226	35	122	5.39e-13	67.8
MsaG025254	AT2G36610.1	39.796	98	51	1	135	224	70	167	1.14e-12	65.9
MsaG025254	AT4G36740.1	43.678	87	49	0	136	222	55	141	6.28e-12	64.3
MsaG025254	AT4G36740.2	43.678	87	49	0	136	222	56	142	6.43e-12	64.3
MsaG025254	AT5G53980.1	42.391	92	53	0	131	222	6	97	4.46e-11	60.5
MsaG025254	AT2G18550.1	43.678	87	49	0	136	222	61	147	6.09e-11	61.6

Find 63 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTATTGAAAAGGAAGATTT+TGG	0.211828	5:+5805787	MsaT025254.1:CDS
TAGAATAATTGATTGGTCTT+TGG	0.254387	5:-5806421	None:intergenic
AGATTAAGGTATTGAGGATT+TGG	0.288551	5:-5805844	None:intergenic
GGAAATGGACGTGGGTGATT+TGG	0.317565	5:-5807194	None:intergenic
TTGAAGAAGACATAAGATTA+AGG	0.336048	5:-5805858	None:intergenic
ATTGGCACTTGCAAAACAAT+TGG	0.337338	5:+5806575	MsaT025254.1:CDS
GCCCATGGTCCCACAGGAAA+TGG	0.346020	5:-5807209	None:intergenic
TTTGAGGGAAGCTCAAACTT+AGG	0.385146	5:-5805816	None:intergenic
GTGCTAGACAAGTTGAAGTT+TGG	0.390504	5:+5806604	MsaT025254.1:CDS
AAGAAGACGCTGAAACTTCA+AGG	0.402562	5:+5806382	MsaT025254.1:CDS
GACATAAGATTAAGGTATTG+AGG	0.421167	5:-5805850	None:intergenic
TCTCTTCTAGAATAATTGAT+TGG	0.421188	5:-5806428	None:intergenic
GATGTGAATCGGTTACCTTC+TGG	0.421492	5:+5806222	MsaT025254.1:CDS
TTTACATGCAGATCGGGATT+CGG	0.433835	5:+5806155	MsaT025254.1:intron
ACACTTGAAACGGTGCTGTT+TGG	0.443597	5:-5806279	None:intergenic
TTGGCACTTGCAAAACAATT+GGG	0.452760	5:+5806576	MsaT025254.1:CDS
GGGCACATGGTAAGAGTTGT+TGG	0.457834	5:-5807104	None:intergenic
GTCCATTTCCTGTGGGACCA+TGG	0.458770	5:+5807207	MsaT025254.1:CDS
TGAAAATAGACGGTTACAAA+AGG	0.474850	5:+5807022	MsaT025254.1:CDS
TTTACCACTCACACTTGAAA+CGG	0.484021	5:-5806289	None:intergenic
TCCATTTCCTGTGGGACCAT+GGG	0.490692	5:+5807208	MsaT025254.1:CDS
TTAAGGTATTGAGGATTTGG+TGG	0.491711	5:-5805841	None:intergenic
AGAAGCGAAAGAGAAGTTAC+AGG	0.494456	5:+5806312	MsaT025254.1:CDS
GCAGCATCAACGGCAGATGA+TGG	0.502736	5:-5807149	None:intergenic
ATTGAAAGTAGAAGGAGAAG+AGG	0.503918	5:-5805887	None:intergenic
ATCCGTACAAGTTAAGATCT+AGG	0.515404	5:-5807275	None:intergenic
TCATTTGCATGTAGAATTGT+GGG	0.520585	5:-5807076	None:intergenic
GTCATTTGCATGTAGAATTG+TGG	0.521859	5:-5807077	None:intergenic
TCGATTCCTCGGAGAAGTAA+AGG	0.525636	5:-5806201	None:intergenic
TCCGAGGAATCGATGTGAAT+CGG	0.527394	5:+5806211	MsaT025254.1:CDS
GCTGTGGCCCATGGTCCCAC+AGG	0.538822	5:-5807215	None:intergenic
TCAAAGGTCCTATGTTGGAT+AGG	0.542840	5:-5807242	None:intergenic
ACGACGCGTGGCAGCATCAA+CGG	0.548952	5:-5807159	None:intergenic
ATGGAAAGAGATTGTTCAAG+AGG	0.550270	5:+5806348	MsaT025254.1:CDS
TGCCACGCGTCGTCATCCTA+TGG	0.554799	5:+5807169	MsaT025254.1:CDS
ACCGATTCACATCGATTCCT+CGG	0.556594	5:-5806212	None:intergenic
CGTGGGTGATTTGGAGCCAT+AGG	0.560215	5:-5807185	None:intergenic
CACATGGTAAGAGTTGTTGG+TGG	0.564954	5:-5807101	None:intergenic
ATAGGAGCAGCTGTGGCCCA+TGG	0.566808	5:-5807224	None:intergenic
GGTCCCACAGGAAATGGACG+TGG	0.582860	5:-5807203	None:intergenic
GGTTTCAAAATCGTAGAGCA+AGG	0.583437	5:+5806625	MsaT025254.1:CDS
ACAGCAACACGCTCACAAGA+GGG	0.586849	5:-5807125	None:intergenic
ATGTTGGATAGGAGCAGCTG+TGG	0.588541	5:-5807231	None:intergenic
CAGCTGCTCCTATCCAACAT+AGG	0.588914	5:+5807234	MsaT025254.1:CDS
GTGTATCAAAGGTCCTATGT+TGG	0.591308	5:-5807247	None:intergenic
CACCCACGTCCATTTCCTGT+GGG	0.600865	5:+5807200	MsaT025254.1:CDS
AACAGCAACACGCTCACAAG+AGG	0.612400	5:-5807126	None:intergenic
ATCTAACGGATGAAAATAGA+CGG	0.614300	5:+5807012	MsaT025254.1:CDS
TCACCCACGTCCATTTCCTG+TGG	0.615645	5:+5807199	MsaT025254.1:CDS
TCTAGGACGGAGTGTATCAA+AGG	0.615968	5:-5807258	None:intergenic
TCTTCACAATCAGCACCAGA+AGG	0.621185	5:-5806237	None:intergenic
ACGCTCACAAGAGGGGCACA+TGG	0.624285	5:-5807117	None:intergenic
GCTGATTGTGAAGAAGAAGC+AGG	0.645646	5:+5806246	MsaT025254.1:CDS
GTACAAGTTAAGATCTAGGA+CGG	0.655097	5:-5807271	None:intergenic
AGCCATAGGATGACGACGCG+TGG	0.657092	5:-5807171	None:intergenic
TACAGGTGAAGATCTTGACA+TGG	0.657130	5:+5806329	MsaT025254.1:CDS
CAGCAACACGCTCACAAGAG+GGG	0.670133	5:-5807124	None:intergenic
AGCACCGTTTCAAGTGTGAG+TGG	0.674395	5:+5806285	MsaT025254.1:CDS
AGACATGCAGAATCGAAGAA+CGG	0.674956	5:+5806178	MsaT025254.1:CDS
GAACGGCCTTTACTTCTCCG+AGG	0.691005	5:+5806195	MsaT025254.1:CDS
GTCCCACAGGAAATGGACGT+GGG	0.703771	5:-5807202	None:intergenic
CATTTGCATGTAGAATTGTG+GGG	0.706166	5:-5807075	None:intergenic
AAGATGCTGTGAGAATCTAA+CGG	0.718794	5:+5806998	MsaT025254.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	5805784	5807286	5805784	ID=MsaG025254
Chr5	mRNA	5805784	5807286	5805784	ID=MsaT025254.1;Parent=MsaG025254
Chr5	exon	5805784	5805949	5805784	ID=MsaT025254.1.exon1;Parent=MsaT025254.1
Chr5	CDS	5805784	5805949	5805784	ID=cds.MsaT025254.1;Parent=MsaT025254.1
Chr5	exon	5806166	5806479	5806166	ID=MsaT025254.1.exon2;Parent=MsaT025254.1
Chr5	CDS	5806166	5806479	5806166	ID=cds.MsaT025254.1;Parent=MsaT025254.1
Chr5	exon	5806567	5806646	5806567	ID=MsaT025254.1.exon3;Parent=MsaT025254.1
Chr5	CDS	5806567	5806646	5806567	ID=cds.MsaT025254.1;Parent=MsaT025254.1
Chr5	exon	5806956	5807286	5806956	ID=MsaT025254.1.exon4;Parent=MsaT025254.1
Chr5	CDS	5806956	5807286	5806956	ID=cds.MsaT025254.1;Parent=MsaT025254.1

Gene Sequence

>MsaT025254.1

ATGAGTATTGAAAAGGAAGATTTTGGTTTGAGCCTAAGTTTGAGCTTCCCTCAAAATCCACCAAATCCTCAATACCTTAATCTTATGTCTTCTTCAACTCATTCCTCTTCTCCTTCTACTTTCAATCCTCAAAAACCTTCTTGGAATGATGTTTTTACTTCTTCAGATCGGGATTCGGAGACATGCAGAATCGAAGAACGGCCTTTACTTCTCCGAGGAATCGATGTGAATCGGTTACCTTCTGGTGCTGATTGTGAAGAAGAAGCAGGAGTTTCATCACCAAACAGCACCGTTTCAAGTGTGAGTGGTAAAAGAAGCGAAAGAGAAGTTACAGGTGAAGATCTTGACATGGAAAGAGATTGTTCAAGAGGAATCAGTGATGAAGAAGACGCTGAAACTTCAAGGAAAAAACTTAGACTCACCAAAGACCAATCAATTATTCTAGAAGAGAGTTTCAAAGAACACAACACTCTTAACCCCAAACAAAAATTGGCACTTGCAAAACAATTGGGACTTCGTGCTAGACAAGTTGAAGTTTGGTTTCAAAATCGTAGAGCAAGGACTAAGTTGAAGCAAACAGAAGTAGATTGTGAGTTTTTGAAAAGATGCTGTGAGAATCTAACGGATGAAAATAGACGGTTACAAAAGGAAGTGCAAGAGTTAAGAGCATTGAAACTTTCCCCACAATTCTACATGCAAATGACACCACCAACAACTCTTACCATGTGCCCCTCTTGTGAGCGTGTTGCTGTTCCATCATCTGCCGTTGATGCTGCCACGCGTCGTCATCCTATGGCTCCAAATCACCCACGTCCATTTCCTGTGGGACCATGGGCCACAGCTGCTCCTATCCAACATAGGACCTTTGATACACTCCGTCCTAGATCTTAA

Protein sequence

>MsaT025254.1

MSIEKEDFGLSLSLSFPQNPPNPQYLNLMSSSTHSSSPSTFNPQKPSWNDVFTSSDRDSETCRIEERPLLLRGIDVNRLPSGADCEEEAGVSSPNSTVSSVSGKRSEREVTGEDLDMERDCSRGISDEEDAETSRKKLRLTKDQSIILEESFKEHNTLNPKQKLALAKQLGLRARQVEVWFQNRRARTKLKQTEVDCEFLKRCCENLTDENRRLQKEVQELRALKLSPQFYMQMTPPTTLTMCPSCERVAVPSSAVDAATRRHPMAPNHPRPFPVGPWATAAPIQHRTFDTLRPRS*