AlfalfaGEDB | MsaG025281

M. sativa ssp. caerulea / MsaG025281

KEGG: -
GO: GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001085 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00320.30

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025281	XP_003611383.2	94.389	499	11	2	1	482	1	499	0.0	968

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025281	sp\|Q8W4H1\|GAT26_ARATH	49.126	515	199	14	1	459	1	508	3.33e-132	395

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025281	tr\|G7JYK7\|G7JYK7_MEDTR	94.389	499	11	2	1	482	1	499	0.0	968

TFs/TRs:

Gene ID	Type	Classification
MsaG025281	TF	C2C2-GATA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025281	MtrunA17_Chr4g0055611	63.842	531	126	9	1	468	1	528	0.0	622
MsaG025281	MtrunA17_Chr5g0399781	98.394	249	4	0	1	249	1	249	0.0	518
MsaG025281	MtrunA17_Chr5g0399791	92.181	243	8	1	251	482	12	254	1.59e-160	454

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025281	AT4G17570.3	49.709	515	200	13	1	459	1	512	6.67e-138	407
MsaG025281	AT4G17570.1	49.126	515	199	14	1	459	1	508	3.39e-133	395
MsaG025281	AT4G17570.2	48.703	501	198	13	15	459	27	524	1.01e-125	376
MsaG025281	AT5G47140.1	47.658	491	202	14	1	459	1	468	1.67e-118	356

Find 90 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTCTTTGTGATAATTCTTT+TGG	0.201366	5:-6060492	None:intergenic
TATCTTCATCAAAAGCCTTT+TGG	0.231803	5:-6057858	None:intergenic
AGCTCTCTTTCATTTGATAA+TGG	0.271694	5:+6059031	MsaT025281.1:CDS
ACCAAATTGTGCACAGCTTT+CGG	0.284475	5:-6057920	None:intergenic
AGTGACGCATATTCTTATTC+TGG	0.322140	5:+6059067	MsaT025281.1:CDS
CATCAGGAGGCAAAGGAAAT+AGG	0.324121	5:-6060532	None:intergenic
TCCGAAAGCTGTGCACAATT+TGG	0.330008	5:+6057919	MsaT025281.1:CDS
GACCTGCTCAGTCAGTGGTC+TGG	0.355510	5:+6058759	MsaT025281.1:CDS
AACCACTATAATGTAGTATC+TGG	0.362580	5:+6057805	MsaT025281.1:CDS
ACCTGCTCAGTCAGTGGTCT+GGG	0.368180	5:+6058760	MsaT025281.1:CDS
TTGTTGTTTGTGCAGCCTTA+AGG	0.373323	5:+6059759	MsaT025281.1:intron
ATGAGTTGTCAAAGTCGAAA+TGG	0.378385	5:+6059911	MsaT025281.1:CDS
TAAGAATATGCGTCACTCAT+TGG	0.378524	5:-6059061	None:intergenic
AGCAGAAATCAGAACTTTCC+AGG	0.401146	5:+6060139	MsaT025281.1:CDS
AAGAATATGCGTCACTCATT+GGG	0.411487	5:-6059060	None:intergenic
TTGCATAGTATTGGCTTCTC+TGG	0.427042	5:-6057622	None:intergenic
GCACACCACTTTGGCGCAAT+GGG	0.430676	5:+6057596	MsaT025281.1:CDS
CCACATGCATTGCATAGTAT+TGG	0.431658	5:-6057631	None:intergenic
ATGAGTAATCGATCAAGTTC+AGG	0.432484	5:+6057880	MsaT025281.1:CDS
CCAATGGTCTCTGTCGAGAT+AGG	0.442024	5:+6058947	MsaT025281.1:CDS
TTACACACCTTTACATGCTC+GGG	0.442559	5:+6057687	MsaT025281.1:CDS
CTGCACCCAACTTCATACTT+TGG	0.442894	5:+6060659	MsaT025281.1:CDS
TTTCAGCAGCTTCTTGCAAA+AGG	0.446745	5:+6059823	MsaT025281.1:CDS
TCTCTTCACATTTGCAACAT+TGG	0.446975	5:-6060107	None:intergenic
ATGCATGTGGTTCGCGGTGG+AGG	0.452122	5:+6057644	MsaT025281.1:CDS
GTGAGCTTCTCAACAGAAGA+TGG	0.452294	5:-6058839	None:intergenic
AGCAAGTGTGAGCTTGCATC+AGG	0.455435	5:-6060548	None:intergenic
TGCAGCCTTAAGGTCATGTT+CGG	0.457133	5:+6059769	MsaT025281.1:intron
AAGAAGTGAACTTGCTCAAA+CGG	0.458776	5:+6057776	MsaT025281.1:CDS
ATTACACACCTTTACATGCT+CGG	0.466186	5:+6057686	MsaT025281.1:CDS
GGCACACCACTTTGGCGCAA+TGG	0.466586	5:+6057595	MsaT025281.1:intron
TGCGGAAAAGAATTATTAGA+AGG	0.469116	5:-6060626	None:intergenic
CTGGAAAGAGTACAAATCCT+TGG	0.472378	5:+6059338	MsaT025281.1:CDS
TTGATGATCAAAGGGCTTCA+AGG	0.472955	5:+6057725	MsaT025281.1:CDS
GTTCGGTAGCTCTCAATTCA+AGG	0.476255	5:+6059786	MsaT025281.1:CDS
ACAGATGCTAGTGATCTAAC+AGG	0.476473	5:+6057946	MsaT025281.1:CDS
TGAGCACCAAAGTATGAAGT+TGG	0.490384	5:-6060665	None:intergenic
GAGTAGAATTCTAACCCATC+AGG	0.493607	5:-6059632	None:intergenic
TTGCAACAGGCACACCACTT+TGG	0.494644	5:+6057587	MsaT025281.1:intron
TGAGTTGTCAAAGTCGAAAT+GGG	0.496810	5:+6059912	MsaT025281.1:CDS
GATGCTATACCAGCAGATCC+TGG	0.497891	5:-6060082	None:intergenic
TCGGATGTTCCAGGATCTGC+TGG	0.499260	5:+6060073	MsaT025281.1:CDS
TTTCAGTGACAAGTCTCAGC+TGG	0.499546	5:+6059319	MsaT025281.1:intron
CGATGCAGAGATGTAAAAGT+AGG	0.503787	5:+6060041	MsaT025281.1:intron
GCGCCTTTGATATCTCTTTG+CGG	0.508397	5:+6059845	MsaT025281.1:CDS
GACATGGAAGTGTTCTCATT+AGG	0.509357	5:+6058969	MsaT025281.1:CDS
CATAGTATTGGCTTCTCTGG+TGG	0.517941	5:-6057619	None:intergenic
GAAAGCTGTGCACAATTTGG+TGG	0.523222	5:+6057922	MsaT025281.1:CDS
AGCTACCGAACATGACCTTA+AGG	0.523689	5:-6059774	None:intergenic
CTCCAGATACTACATTATAG+TGG	0.528306	5:-6057807	None:intergenic
TGCTGAGCTACTACTAGTGC+TGG	0.529471	5:-6060690	None:intergenic
ATTATTGAAGTTTCTTCCTG+TGG	0.538673	5:+6059592	MsaT025281.1:CDS
TTACAACCAGGGATTCCAAA+AGG	0.539474	5:+6057843	MsaT025281.1:CDS
GAGCACCAAAGTATGAAGTT+GGG	0.542626	5:-6060664	None:intergenic
TCGTCGCGAGCTACATAACT+AGG	0.544739	5:-6058995	None:intergenic
TTCTGAGACATGGGTAAACA+AGG	0.546162	5:+6056210	None:intergenic
TTATGAAAGTGAAGCACCAA+TGG	0.548282	5:+6058931	MsaT025281.1:CDS
GTTTCAGCCCGAGCATGTAA+AGG	0.551747	5:-6057694	None:intergenic
GGTTCGCGGTGGAGGACAAA+GGG	0.557202	5:+6057652	MsaT025281.1:CDS
GATACTGCTAAACTTCCTGA+TGG	0.560155	5:+6059617	MsaT025281.1:CDS
TCCCAGACCACTGACTGAGC+AGG	0.563264	5:-6058761	None:intergenic
TGGTTCGCGGTGGAGGACAA+AGG	0.567138	5:+6057651	MsaT025281.1:CDS
TTTAGGACCTGCTCAGTCAG+TGG	0.572891	5:+6058754	MsaT025281.1:intron
AGTTTAGCAGTATCAACCAC+AGG	0.576633	5:-6059608	None:intergenic
CAGAGATGAAGACAATAATG+AGG	0.584067	5:+6060465	MsaT025281.1:intron
CACTTGTAACTCCACAGTGA+TGG	0.587666	5:-6056239	None:intergenic
GAGCTTGCATCAGGAGGCAA+AGG	0.591960	5:-6060539	None:intergenic
ATTTAAATCTACAAAACACA+AGG	0.595356	5:-6059375	None:intergenic
GATGCAGAGATGTAAAAGTA+GGG	0.597722	5:+6060042	MsaT025281.1:intron
GTCTCTGTCGAGATAGGACA+TGG	0.606101	5:+6058953	MsaT025281.1:CDS
ATACTGCTAAACTTCCTGAT+GGG	0.607368	5:+6059618	MsaT025281.1:CDS
AGCTCGCGACGAAGAGTCTG+AGG	0.607702	5:+6059006	MsaT025281.1:CDS
TATGCAATGCATGTGGTTCG+CGG	0.611941	5:+6057638	MsaT025281.1:CDS
CCTATCTCGACAGAGACCAT+TGG	0.618260	5:-6058947	None:intergenic
GTAACTCCACAGTGATGGCA+AGG	0.624432	5:-6056234	None:intergenic
CTTGCATCTGATTACAACCA+GGG	0.633133	5:+6057832	MsaT025281.1:CDS
CACTATAATGTAGTATCTGG+AGG	0.635640	5:+6057808	MsaT025281.1:CDS
AGGGTGAATCATGATTTCCA+AGG	0.637136	5:-6059355	None:intergenic
AAGTGTGAGCTTGCATCAGG+AGG	0.639748	5:-6060545	None:intergenic
ACTTGCATCTGATTACAACC+AGG	0.649715	5:+6057831	MsaT025281.1:CDS
GTTGTCAAAGTCGAAATGGG+TGG	0.657876	5:+6059915	MsaT025281.1:CDS
CATCCGCAAAGAGATATCAA+AGG	0.674445	5:-6059848	None:intergenic
CCAATACTATGCAATGCATG+TGG	0.675078	5:+6057631	MsaT025281.1:CDS
GGTGGCCCATTGCGCCAAAG+TGG	0.675260	5:-6057601	None:intergenic
TTTAAATCTACAAAACACAA+GGG	0.680510	5:-6059374	None:intergenic
GGGTGCAGAAGTTCTGCCTG+CGG	0.683148	5:-6060644	None:intergenic
GGTGGAACACTATCATAACA+AGG	0.694043	5:+6059933	MsaT025281.1:CDS
GCAATGCATGTGGTTCGCGG+TGG	0.695010	5:+6057641	MsaT025281.1:CDS
CAAGGACCTTGCCATCACTG+TGG	0.727560	5:+6056228	MsaT025281.1:CDS
TAAACTATGAAGAATTTACG+AGG	0.736561	5:+6059543	MsaT025281.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	6056219	6060736	6056219	ID=MsaG025281
Chr5	mRNA	6056219	6060736	6056219	ID=MsaT025281.1;Parent=MsaG025281
Chr5	exon	6056219	6056258	6056219	ID=MsaT025281.1.exon1;Parent=MsaT025281.1
Chr5	CDS	6056219	6056258	6056219	ID=cds.MsaT025281.1;Parent=MsaT025281.1
Chr5	exon	6057596	6057967	6057596	ID=MsaT025281.1.exon2;Parent=MsaT025281.1
Chr5	CDS	6057596	6057967	6057596	ID=cds.MsaT025281.1;Parent=MsaT025281.1
Chr5	exon	6058759	6059093	6058759	ID=MsaT025281.1.exon3;Parent=MsaT025281.1
Chr5	CDS	6058759	6059093	6058759	ID=cds.MsaT025281.1;Parent=MsaT025281.1
Chr5	exon	6059332	6059397	6059332	ID=MsaT025281.1.exon4;Parent=MsaT025281.1
Chr5	CDS	6059332	6059397	6059332	ID=cds.MsaT025281.1;Parent=MsaT025281.1
Chr5	exon	6059536	6059639	6059536	ID=MsaT025281.1.exon5;Parent=MsaT025281.1
Chr5	CDS	6059536	6059639	6059536	ID=cds.MsaT025281.1;Parent=MsaT025281.1
Chr5	exon	6059774	6059869	6059774	ID=MsaT025281.1.exon6;Parent=MsaT025281.1
Chr5	CDS	6059774	6059869	6059774	ID=cds.MsaT025281.1;Parent=MsaT025281.1
Chr5	exon	6059900	6059954	6059900	ID=MsaT025281.1.exon7;Parent=MsaT025281.1
Chr5	CDS	6059900	6059954	6059900	ID=cds.MsaT025281.1;Parent=MsaT025281.1
Chr5	exon	6060049	6060160	6060049	ID=MsaT025281.1.exon8;Parent=MsaT025281.1
Chr5	CDS	6060049	6060160	6060049	ID=cds.MsaT025281.1;Parent=MsaT025281.1
Chr5	exon	6060468	6060736	6060468	ID=MsaT025281.1.exon9;Parent=MsaT025281.1
Chr5	CDS	6060468	6060736	6060468	ID=cds.MsaT025281.1;Parent=MsaT025281.1

Gene Sequence

>MsaT025281.1

ATGGGTAAACAAGGACCTTGCCATCACTGTGGAGTTACAAGCACACCACTTTGGCGCAATGGGCCACCAGAGAAGCCAATACTATGCAATGCATGTGGTTCGCGGTGGAGGACAAAGGGAACACTTGCAAATTACACACCTTTACATGCTCGGGCTGAAACTGATGATTTTGATGATCAAAGGGCTTCAAGGACGAAGAGCATATCGTTAAATAAGAACAAAGAAGTGAACTTGCTCAAACGGAAGCAGAACCACTATAATGTAGTATCTGGAGGACTTGCATCTGATTACAACCAGGGATTCCAAAAGGCTTTTGATGAAGATATGAGTAATCGATCAAGTTCAGGATCAGCATTGTCTAACTCCGAAAGCTGTGCACAATTTGGTGGCACAGATGCTAGTGATCTAACAGGACCTGCTCAGTCAGTGGTCTGGGATACCACTCCCACAGTGCCTTCAAAAAAGAGGACATGTGTTGGTCGTGCAAAGCAGCCATCTTCTGTTGAGAAGCTCACAAAAGATTTATGCACTATTCTTCATGAACAACAATCTTGTTTTTCTGAATCTTCTGATGGGGATCTTCTTTATGAAAGTGAAGCACCAATGGTCTCTGTCGAGATAGGACATGGAAGTGTTCTCATTAGGCATCCTAGTTATGTAGCTCGCGACGAAGAGTCTGAGGCTAGCTCTCTTTCATTTGATAATGGACTATACCCAATGAGTGACGCATATTCTTATTCTGGCGCCTCTCAGCTGGAAAGAGTACAAATCCTTGGAAATCATGATTCACCCTTGTGTTTTGTAGATTTAAATGATGTAGTAAACTATGAAGAATTTACGAGGAACTTGTCATATGAAGAGCAGCAACAATTATTGAAGTTTCTTCCTGTGGTTGATACTGCTAAACTTCCTGATGGCCTTAAGGTCATGTTCGGTAGCTCTCAATTCAAGGAGAACTTAACTAATTTTCAGCAGCTTCTTGCAAAAGGCGCCTTTGATATCTCTTTGCGGATATATGCACTAAATGAGTTGTCAAAGTCGAAATGGGTGGAACACTATCATAACAAGAGATGTAAAAGTAGGGCTGAAAAATCGGATGTTCCAGGATCTGCTGGTATAGCATCAACCAATGTTGCAAATGTGAAGAGAATGCGTGATAGCAGAAATCAGAACTTTCCAGAGATGAAGACAATAATGAGGAGCCCCAAAAGAATTATCACAAAGACTAGTTATAATCCAAAAAGCCTATTTCCTTTGCCTCCTGATGCAAGCTCACACTTGCTAGATTCTTTTAACTTTGTTGAGGAGAGTTCTGATCAAGATCTGCTGCTAGAAGTGCCTTCTAATAATTCTTTTCCGCAGGCAGAACTTCTGCACCCAACTTCATACTTTGGTGCTCAAGCCAGCACTAGTAGTAGCTCAGCATACTCACATTTTGTGAACCATTAA

Protein sequence

>MsaT025281.1

MGKQGPCHHCGVTSTPLWRNGPPEKPILCNACGSRWRTKGTLANYTPLHARAETDDFDDQRASRTKSISLNKNKEVNLLKRKQNHYNVVSGGLASDYNQGFQKAFDEDMSNRSSSGSALSNSESCAQFGGTDASDLTGPAQSVVWDTTPTVPSKKRTCVGRAKQPSSVEKLTKDLCTILHEQQSCFSESSDGDLLYESEAPMVSVEIGHGSVLIRHPSYVARDEESEASSLSFDNGLYPMSDAYSYSGASQLERVQILGNHDSPLCFVDLNDVVNYEEFTRNLSYEEQQQLLKFLPVVDTAKLPDGLKVMFGSSQFKENLTNFQQLLAKGAFDISLRIYALNELSKSKWVEHYHNKRCKSRAEKSDVPGSAGIASTNVANVKRMRDSRNQNFPEMKTIMRSPKRIITKTSYNPKSLFPLPPDASSHLLDSFNFVEESSDQDLLLEVPSNNSFPQAELLHPTSYFGAQASTSSSSAYSHFVNH*