AlfalfaGEDB | MsaG025290

M. sativa ssp. caerulea / MsaG025290

KEGG: K13464
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003712 GO:0003714 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009791 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010556 GO:0010558 GO:0010605 GO:0010646 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045934 GO:0048229 GO:0048367 GO:0048519 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0080134 GO:0090567 GO:0098542 GO:0140110 GO:1900067 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000022 GO:2001141
PFAM: PF06200.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025290	XP_013453448.3	79.137	139	28	1	152	289	6	144	3.45e-71	227
MsaG025290	XP_013453448.3	87.037	108	13	1	31	137	6	113	1.11e-59	198

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025290	sp\|Q84R94\|TI10A_ORYSJ	39.735	151	79	4	150	289	65	214	8.21e-22	95.1
MsaG025290	sp\|Q84R94\|TI10A_ORYSJ	42.188	128	54	4	26	137	58	181	1.18e-17	83.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025290	tr\|A0A396HSW0\|A0A396HSW0_MEDTR	79.137	139	28	1	152	289	6	144	2.26e-71	227
MsaG025290	tr\|A0A396HSW0\|A0A396HSW0_MEDTR	87.037	108	13	1	31	137	6	113	7.43e-60	197

TFs/TRs:

Gene ID	Type	Classification
MsaG025290	TF	Tify

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000982	MsaG025290	0.810628	1.795144e-50	3.112881e-48
MsaG004462	MsaG025290	0.803986	4.522789e-49	6.697425e-47
MsaG004760	MsaG025290	0.807294	9.208917e-50	1.473476e-47
MsaG005401	MsaG025290	0.812115	8.564697e-51	1.540267e-48
MsaG006561	MsaG025290	0.840836	1.266895e-57	5.076991e-55
MsaG011528	MsaG025290	0.826469	4.749652e-54	1.244463e-51
MsaG018225	MsaG025290	0.813813	3.650383e-51	6.849378e-49
MsaG018813	MsaG025290	0.833212	1.100505e-55	3.495997e-53
MsaG018988	MsaG025290	0.800256	2.623496e-48	3.567654e-46
MsaG019303	MsaG025290	0.836698	1.470540e-56	5.183476e-54
MsaG021325	MsaG025290	0.802708	8.296328e-49	1.192772e-46
MsaG023971	MsaG025290	0.800996	1.856160e-48	2.566696e-46
MsaG025290	MsaG030955	0.806210	1.556864e-49	2.428213e-47
MsaG025290	MsaG034875	0.800103	2.817830e-48	3.818701e-46
MsaG025290	MsaG036047	0.849846	4.730519e-60	2.550330e-57
MsaG025290	MsaG039160	0.803927	4.651178e-49	6.878195e-47
MsaG025290	MsaG041112	0.820677	1.060346e-52	2.374122e-50
MsaG025290	MsaG045487	0.827636	2.504165e-54	6.778434e-52
MsaG025290	MsaG046512	0.801213	1.677108e-48	2.330435e-46
MsaG025290	MsaG046546	0.811984	9.146168e-51	1.639588e-48
MsaG025290	MsaG046807	0.804044	4.399871e-49	6.524481e-47
MsaG025290	MsaG001986	0.831334	3.193656e-55	9.603373e-53
MsaG025290	MsaG003742	0.824194	1.630370e-53	4.011922e-51
MsaG025290	MsaG000974	0.807929	6.763570e-50	1.098831e-47
MsaG025290	MsaG015507	0.844300	1.540072e-58	6.896443e-56
MsaG025290	MsaG030315	0.838142	6.298930e-57	2.320326e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025290	MtrunA17_Chr5g0399851	79.137	139	28	1	152	289	6	144	4.35e-75	227
MsaG025290	MtrunA17_Chr5g0399851	87.037	108	13	1	31	137	6	113	1.43e-63	197
MsaG025290	MtrunA17_Chr5g0399861	71.739	138	30	4	1	137	1	130	6.63e-62	194
MsaG025290	MtrunA17_Chr5g0399861	65.132	152	45	3	147	297	24	168	2.66e-61	193
MsaG025290	MtrunA17_Chr5g0399881	72.464	138	29	4	1	137	1	130	8.15e-62	194
MsaG025290	MtrunA17_Chr5g0399881	66.438	146	41	3	147	290	24	163	6.20e-58	184
MsaG025290	MtrunA17_Chr2g0301131	41.935	155	53	5	156	284	102	245	2.69e-27	107
MsaG025290	MtrunA17_Chr2g0301131	43.066	137	43	4	27	137	99	226	3.27e-26	104
MsaG025290	MtrunA17_Chr4g0070271	37.719	114	48	3	157	270	91	181	1.02e-15	74.7
MsaG025290	MtrunA17_Chr4g0070271	39.796	98	36	2	40	137	95	169	8.89e-15	72.0
MsaG025290	MtrunA17_Chr4g0069721	32.479	117	61	3	163	265	41	153	1.55e-11	62.4
MsaG025290	MtrunA17_Chr8g0344481	30.000	130	87	1	147	272	85	214	3.34e-11	62.4
MsaG025290	MtrunA17_Chr2g0302041	34.821	112	63	4	167	268	58	169	8.93e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025290	AT3G43440.1	31.140	228	106	7	46	269	45	225	1.22e-21	92.0
MsaG025290	AT1G19180.2	40.000	140	72	5	156	287	49	184	1.19e-19	85.5
MsaG025290	AT1G19180.2	45.133	113	51	5	39	145	53	160	1.64e-18	82.4
MsaG025290	AT1G74950.1	41.129	124	58	4	156	266	108	229	2.90e-19	85.9
MsaG025290	AT1G74950.1	38.333	120	62	3	39	147	112	230	2.88e-17	80.1
MsaG025290	AT1G19180.1	40.000	140	72	6	156	287	115	250	6.58e-19	84.7
MsaG025290	AT1G19180.1	43.966	116	54	5	39	148	119	229	8.27e-18	81.6
MsaG025290	AT1G19180.3	40.000	140	72	6	156	287	115	250	6.58e-19	84.7
MsaG025290	AT1G19180.3	43.966	116	54	5	39	148	119	229	8.27e-18	81.6
MsaG025290	AT1G74950.2	39.091	110	56	2	39	137	112	221	6.15e-17	78.6
MsaG025290	AT1G74950.2	39.655	116	55	4	156	258	108	221	5.54e-15	73.2
MsaG025290	AT5G20900.1	39.370	127	67	5	153	269	41	167	2.01e-14	70.9

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTACAAGAGGTTTCAAATTT+AGG	0.093614	5:+6169372	MsaT025290.1:CDS
CCATCTACACCTGTTATTTA+TGG	0.119392	5:+6173341	MsaT025290.1:CDS
TCATCCACACCTGTTATTTA+TGG	0.190028	5:+6169727	MsaT025290.1:CDS
TTGTAGTCTTGTGGTGATTT+TGG	0.235832	5:-6181170	None:intergenic
ATTCATCAATTAGCTTATTA+AGG	0.237455	5:-6181124	None:intergenic
GGCTTCACTTCATCGGTTCT+TGG	0.289096	5:+6176388	MsaT025290.1:CDS
AGCTTCACTTCATCGGTTCT+TGG	0.292747	5:+6169881	MsaT025290.1:CDS
ATTGTCATTTGGGCACTTCT+AGG	0.305926	5:-6173134	None:intergenic
TTAGGGTTACTTGTTTGATA+TGG	0.321660	5:-6181098	None:intergenic
AAGCTACAAAGACATATTCT+AGG	0.321804	5:+6186984	MsaT025290.1:CDS
TTTCAAATTTAGGAAATTCC+AGG	0.358166	5:+6169382	MsaT025290.1:CDS
TTGAGAGTTAGTAGTTCCTT+TGG	0.375053	5:-6169617	None:intergenic
GGGGTGACTCTGAGCAAATC+TGG	0.380764	5:-6173276	None:intergenic
AGTGAAGCTTTCCTAGTCAT+TGG	0.425048	5:-6169867	None:intergenic
TCGAGAGGTAGTAGTTCCTT+TGG	0.433772	5:-6173231	None:intergenic
GAATTTCTAGCAAGAAATGT+TGG	0.438384	5:-6169682	None:intergenic
ATTCAGATTTATCAATTACT+AGG	0.446451	5:+6176363	MsaT025290.1:intron
CCATAAATAACAGGTGTAGA+TGG	0.461679	5:-6173341	None:intergenic
ATTGTCATTTGTGCACATCT+AGG	0.463250	5:-6169520	None:intergenic
TTCATCGGTTCTTGGAGAAA+AGG	0.463618	5:+6169889	MsaT025290.1:CDS
CATTCTGATTTGTAGTCTTG+TGG	0.466825	5:-6181179	None:intergenic
TTCATCGGTTCTTGGAAAGA+AGG	0.486928	5:+6176396	MsaT025290.1:CDS
ATATAATCGTTGTCGCTACC+AGG	0.492558	5:+6171803	MsaT025290.1:CDS
AGAGTTAGTAGTTCCTTTGG+AGG	0.509053	5:-6169614	None:intergenic
ATCTATGTCATGTCTCTCCT+TGG	0.510141	5:+6181145	MsaT025290.1:CDS
AGATTTATCAATTACTAGGA+AGG	0.513206	5:+6176367	MsaT025290.1:intron
AATTTCTAGCAAGAAATGTT+GGG	0.519439	5:-6169681	None:intergenic
CTAGGAAGGCTTCACTTCAT+CGG	0.521487	5:+6176381	MsaT025290.1:CDS
CTAGGAAAGCTTCACTTCAT+CGG	0.529148	5:+6169874	MsaT025290.1:CDS
TGATACTTGAAGAGAATTGT+TGG	0.532943	5:-6169707	None:intergenic
AATGAAGACTAATATTATCA+AGG	0.534533	5:+6173106	MsaT025290.1:CDS
TGGTATTTGAGCTGAATTGT+TGG	0.539227	5:-6173321	None:intergenic
GAAAATTCAACATAGGTGTA+CGG	0.542214	5:-6169326	None:intergenic
TCGGTTCTTGGAAAGAAGGA+TGG	0.543639	5:+6176400	MsaT025290.1:CDS
TGAAAGCATAGTTATTGTTG+TGG	0.546555	5:-6169642	None:intergenic
AATGAAGGCTAATATTAAGA+AGG	0.546955	5:+6169492	MsaT025290.1:CDS
AGCTACAAAGACATATTCTA+GGG	0.555201	5:+6186985	MsaT025290.1:CDS
AAAATTTCTAGCAAGAAATG+AGG	0.558270	5:-6173297	None:intergenic
GTTTCTGCAGATTTACTGCA+AGG	0.559640	5:+6181072	MsaT025290.1:intron
TCTTGGAGAAAAGGAAGGAT+AGG	0.567398	5:+6169898	MsaT025290.1:CDS
ACATGTTGAAAATTCAACAT+AGG	0.573945	5:-6169333	None:intergenic
AAATTTCTAGCAAGAAATGA+GGG	0.575092	5:-6173296	None:intergenic
TCTTGGAAAGAAGGATGGAT+AGG	0.575095	5:+6176405	MsaT025290.1:CDS
TCCTCATCTCTCTCTACAAG+AGG	0.582418	5:+6169359	MsaT025290.1:CDS
TCGGTTCTTGGAGAAAAGGA+AGG	0.585705	5:+6169893	MsaT025290.1:CDS
TTTACCATAAATAACAGGTG+TGG	0.596443	5:-6169731	None:intergenic
TTTCTGCAGATTTACTGCAA+GGG	0.601135	5:+6181073	MsaT025290.1:intron
TGGAAGCGTAGTTATTGCTG+TGG	0.608871	5:-6173256	None:intergenic
GTTATTGCTGTGGTTTCGAG+AGG	0.614841	5:-6173246	None:intergenic
ATACAGATTTACCAATGACT+AGG	0.627962	5:+6169856	MsaT025290.1:intron
TTCTAGCAAGAAATGTTGGG+TGG	0.637884	5:-6169678	None:intergenic
ACCTCTTGTAGAGAGAGATG+AGG	0.644344	5:-6169360	None:intergenic
AATTTCTAGCAAGAAATGAG+GGG	0.713360	5:-6173295	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	6169312	6187005	6169312	ID=MsaG025290
Chr5	mRNA	6169312	6187005	6169312	ID=MsaT025290.1;Parent=MsaG025290
Chr5	exon	6169312	6169403	6169312	ID=MsaT025290.1.exon1;Parent=MsaT025290.1
Chr5	CDS	6169312	6169403	6169312	ID=cds.MsaT025290.1;Parent=MsaT025290.1
Chr5	exon	6169489	6169748	6169489	ID=MsaT025290.1.exon2;Parent=MsaT025290.1
Chr5	CDS	6169489	6169748	6169489	ID=cds.MsaT025290.1;Parent=MsaT025290.1
Chr5	exon	6169862	6169919	6169862	ID=MsaT025290.1.exon3;Parent=MsaT025290.1
Chr5	CDS	6169862	6169919	6169862	ID=cds.MsaT025290.1;Parent=MsaT025290.1
Chr5	exon	6171780	6171824	6171780	ID=MsaT025290.1.exon4;Parent=MsaT025290.1
Chr5	CDS	6171780	6171824	6171780	ID=cds.MsaT025290.1;Parent=MsaT025290.1
Chr5	exon	6173103	6173362	6173103	ID=MsaT025290.1.exon5;Parent=MsaT025290.1
Chr5	CDS	6173103	6173362	6173103	ID=cds.MsaT025290.1;Parent=MsaT025290.1
Chr5	exon	6176369	6176426	6176369	ID=MsaT025290.1.exon6;Parent=MsaT025290.1
Chr5	CDS	6176369	6176426	6176369	ID=cds.MsaT025290.1;Parent=MsaT025290.1
Chr5	exon	6181082	6181200	6181082	ID=MsaT025290.1.exon7;Parent=MsaT025290.1
Chr5	CDS	6181082	6181200	6181082	ID=cds.MsaT025290.1;Parent=MsaT025290.1
Chr5	exon	6182892	6182917	6182892	ID=MsaT025290.1.exon8;Parent=MsaT025290.1
Chr5	CDS	6182892	6182917	6182892	ID=cds.MsaT025290.1;Parent=MsaT025290.1
Chr5	exon	6186970	6187005	6186970	ID=MsaT025290.1.exon9;Parent=MsaT025290.1
Chr5	CDS	6186970	6187005	6186970	ID=cds.MsaT025290.1;Parent=MsaT025290.1

Gene Sequence

>MsaT025290.1

ATGAATCATTACAACCGTACACCTATGTTGAATTTTCAACATGTATATCCTCATCTCTCTCTACAAGAGGTTTCAAATTTAGGAAATTCCAGTGAAATGAAGGCTAATATTAAGAAGGAAGAGCCTAGATGTGCACAAATGACAATTTTTTATGATGGGAAAGTAATAGTGTTTGATGATATTCCAGCTGAAAAAGTCAAGGACATTATGGTCTTTTCCTCCAAAGGAACTACTAACTCTCAAAACCACAACAATAACTATGCTTTCAGATTTGCTCAGAGCCACCCAACATTTCTTGCTAGAAATTCTGCCAACAATTCTCTTCAAGTATCATCCACACCTGTTATTTATGATTTACCAATGACTAGGAAAGCTTCACTTCATCGGTTCTTGGAGAAAAGGAAGGATAGGGATTTGAGCAGCGAGCATGCGTATATAATCGTTGTCGCTACCAGTGTAATGAAGACTAATATTATCAAGGAAGAGCCTAGAAGTGCCCAAATGACAATTTTTTATGATGGGAAAGTAATTGTGTTTGATGATATTCCAGCTGAAAAAGTCAACGATATCATGGTCTTTTGCACCAAAGGAACTACTACCTCTCGAAACCACAGCAATAACTACGCTTCCAGATTTGCTCAGAGTCACCCCTCATTTCTTGCTAGAAATTTTGCCAACAATTCAGCTCAAATACCATCTACACCTGTTATTTATGATTTATCAATTACTAGGAAGGCTTCACTTCATCGGTTCTTGGAAAGAAGGATGGATAGATTTACTGCAAGGGCGCCATATCAAACAAGTAACCCTAAAAACCTTAATAAGCTAATTGATGAATCTATGTCATGTCTCTCCTTGGCACCAAAATCACCACAAGACTACAAATCAGAATCACTACAAGTTTTTTTTTTCCTTACGGGAATGTATGTTGAAAGCTACAAAGACATATTCTAG

Protein sequence

>MsaT025290.1

MNHYNRTPMLNFQHVYPHLSLQEVSNLGNSSEMKANIKKEEPRCAQMTIFYDGKVIVFDDIPAEKVKDIMVFSSKGTTNSQNHNNNYAFRFAQSHPTFLARNSANNSLQVSSTPVIYDLPMTRKASLHRFLEKRKDRDLSSEHAYIIVVATSVMKTNIIKEEPRSAQMTIFYDGKVIVFDDIPAEKVNDIMVFCTKGTTTSRNHSNNYASRFAQSHPSFLARNFANNSAQIPSTPVIYDLSITRKASLHRFLERRMDRFTARAPYQTSNPKNLNKLIDESMSCLSLAPKSPQDYKSESLQVFFFLTGMYVESYKDIF*