AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG025337


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025337 MtrunA17_Chr5g0400461 98.077 52 1 0 1 52 1 52 5.67e-32 114
MsaG025337 MtrunA17_Chr4g0054791 90.385 52 5 0 1 52 1 52 3.09e-29 107
MsaG025337 MtrunA17_Chr3g0109821 67.925 53 17 0 1 53 1 53 3.84e-22 89.7
MsaG025337 MtrunA17_Chr6g0481091 66.667 51 17 0 1 51 1 51 3.72e-20 81.6
MsaG025337 MtrunA17_Chr5g0433781 64.151 53 19 0 1 53 1 53 3.87e-20 84.0
MsaG025337 MtrunA17_Chr2g0309891 59.615 52 21 0 1 52 1 52 3.61e-18 77.8
MsaG025337 MtrunA17_Chr5g0417001 52.542 59 26 1 8 64 16 74 2.92e-17 75.5
MsaG025337 MtrunA17_Chr6g0453131 50.820 61 28 1 6 64 15 75 4.71e-17 74.7
MsaG025337 MtrunA17_Chr8g0361731 50.847 59 27 1 8 64 16 74 5.64e-17 74.7
MsaG025337 MtrunA17_Chr4g0047841 61.111 54 20 1 6 59 15 67 7.20e-17 73.9
MsaG025337 MtrunA17_Chr5g0442771 50.847 59 27 1 8 64 20 78 1.89e-16 72.8
MsaG025337 MtrunA17_Chr1g0200561 56.250 48 21 0 4 51 17 64 1.96e-16 72.8
MsaG025337 MtrunA17_Chr3g0130731 58.824 51 21 0 2 52 3 53 2.72e-16 72.0
MsaG025337 MtrunA17_Chr2g0318101 57.143 49 21 0 5 53 14 62 3.14e-16 72.4
MsaG025337 MtrunA17_Chr8g0384721 58.824 51 21 0 2 52 3 53 3.26e-16 71.6
MsaG025337 MtrunA17_Chr4g0076731 58.696 46 19 0 8 53 56 101 4.86e-16 72.0
MsaG025337 MtrunA17_Chr4g0056541 56.250 48 21 0 4 51 5 52 5.44e-16 71.6
MsaG025337 MtrunA17_Chr5g0405211 56.250 48 21 0 4 51 6 53 5.67e-16 71.6
MsaG025337 MtrunA17_Chr5g0417791 58.824 51 21 0 6 56 3 53 1.09e-15 70.1
MsaG025337 MtrunA17_Chr7g0214071 53.333 60 28 0 2 61 11 70 1.22e-15 69.7
MsaG025337 MtrunA17_Chr8g0372121 54.902 51 23 0 1 51 1 51 1.41e-15 70.5
MsaG025337 MtrunA17_Chr2g0318081 64.583 48 17 0 5 52 15 62 1.76e-15 70.5
MsaG025337 MtrunA17_Chr4g0018391 54.167 48 22 0 4 51 5 52 1.94e-15 70.1
MsaG025337 MtrunA17_Chr3g0124761 60.417 48 19 0 6 53 7 54 2.31e-15 69.3
MsaG025337 MtrunA17_Chr8g0359921 65.306 49 17 0 5 53 13 61 5.34e-15 68.9
MsaG025337 MtrunA17_Chr3g0131911 48.333 60 31 0 2 61 10 69 5.41e-15 67.8
MsaG025337 MtrunA17_Chr2g0311651 53.846 52 23 1 2 52 17 68 5.88e-15 68.6
MsaG025337 MtrunA17_Chr5g0398971 50.000 48 24 0 4 51 5 52 7.47e-15 68.6
MsaG025337 MtrunA17_Chr7g0252971 51.020 49 24 0 5 53 7 55 8.63e-15 68.2
MsaG025337 MtrunA17_Chr4g0042561 54.902 51 23 0 2 52 5 55 9.72e-15 67.8
MsaG025337 MtrunA17_Chr2g0311661 57.778 45 19 0 8 52 23 67 1.03e-14 67.8
MsaG025337 MtrunA17_Chr4g0050701 53.704 54 25 0 6 59 11 64 4.92e-14 64.7
MsaG025337 MtrunA17_Chr2g0309331 52.000 50 24 0 2 51 6 55 6.99e-14 65.9
MsaG025337 MtrunA17_Chr1g0197351 58.333 48 20 0 4 51 40 87 9.88e-14 65.5
MsaG025337 MtrunA17_Chr8g0346001 50.000 50 25 0 2 51 11 60 1.50e-13 65.1
MsaG025337 MtrunA17_Chr2g0326361 52.083 48 23 0 5 52 13 60 1.63e-13 64.7
MsaG025337 MtrunA17_Chr8g0385991 51.923 52 25 0 2 53 10 61 1.74e-13 63.5
MsaG025337 MtrunA17_Chr7g0263931 57.447 47 20 0 6 52 11 57 2.71e-13 63.9
MsaG025337 MtrunA17_Chr1g0197361 58.696 46 18 1 8 52 10 55 3.51e-13 63.9
MsaG025337 MtrunA17_Chr1g0201021 60.000 45 18 0 8 52 21 65 3.64e-13 63.9
MsaG025337 MtrunA17_Chr6g0484441 51.020 49 24 0 5 53 4 52 4.11e-13 63.5
MsaG025337 MtrunA17_Chr8g0345711 41.758 91 46 3 5 89 11 100 7.66e-13 61.2
MsaG025337 MtrunA17_Chr4g0017921 54.348 46 21 0 6 51 8 53 7.92e-13 62.8
MsaG025337 MtrunA17_Chr1g0194791 56.522 46 19 1 8 52 48 93 1.82e-12 58.5
MsaG025337 MtrunA17_Chr1g0147421 54.000 50 22 1 5 53 26 75 1.89e-12 62.0
MsaG025337 MtrunA17_Chr3g0092671 56.522 46 19 1 8 52 7 52 3.88e-12 61.2
MsaG025337 MtrunA17_Chr2g0316971 55.102 49 22 0 4 52 21 69 4.73e-12 60.8
MsaG025337 MtrunA17_Chr3g0128151 56.250 48 21 0 5 52 21 68 4.81e-12 60.8
MsaG025337 MtrunA17_Chr3g0130101 55.556 45 20 0 8 52 9 53 5.85e-12 60.5
MsaG025337 MtrunA17_Chr3g0139331 55.102 49 22 0 5 53 10 58 1.14e-11 59.7
MsaG025337 MtrunA17_Chr3g0091921 43.939 66 35 1 6 69 16 81 1.43e-11 59.3
MsaG025337 MtrunA17_Chr4g0060931 53.191 47 22 0 6 52 17 63 1.88e-11 58.9
MsaG025337 MtrunA17_Chr5g0428991 53.191 47 22 0 6 52 25 71 2.76e-11 58.5
MsaG025337 MtrunA17_Chr4g0026141 45.902 61 27 2 6 65 5 60 3.86e-11 57.8
MsaG025337 MtrunA17_Chr8g0384201 51.923 52 25 0 1 52 1 52 5.84e-11 57.8
MsaG025337 MtrunA17_Chr1g0183741 46.000 50 27 0 3 52 7 56 6.17e-11 57.4
MsaG025337 MtrunA17_Chr7g0261411 48.000 50 26 0 3 52 7 56 6.36e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025337 AT4G17980.1 86.792 53 7 0 1 53 1 53 7.05e-29 105
MsaG025337 AT4G17980.2 86.792 53 7 0 1 53 1 53 8.58e-29 105
MsaG025337 AT5G46590.2 86.538 52 7 0 1 52 1 52 7.25e-27 100
MsaG025337 AT5G46590.1 86.538 52 7 0 1 52 1 52 7.66e-27 100
MsaG025337 AT1G32510.1 75.000 52 13 0 1 52 1 52 2.77e-23 90.9
MsaG025337 AT3G03200.1 73.585 53 14 0 1 53 1 53 3.00e-23 92.8
MsaG025337 AT5G17260.1 71.698 53 15 0 1 53 1 53 9.96e-23 91.3
MsaG025337 AT1G65910.1 67.925 53 17 0 1 53 1 53 1.52e-21 88.2
MsaG025337 AT1G54330.1 67.308 52 17 0 1 52 1 52 2.61e-20 83.2
MsaG025337 AT3G17730.1 64.706 51 18 0 1 51 1 51 1.32e-19 80.5
MsaG025337 AT2G43000.1 59.322 59 22 1 8 64 20 78 3.70e-18 77.4
MsaG025337 AT2G33480.2 55.224 67 30 0 2 68 11 77 4.73e-17 74.3
MsaG025337 AT2G33480.1 55.224 67 30 0 2 68 11 77 5.05e-17 73.9
MsaG025337 AT5G66300.1 61.224 49 19 0 3 51 9 57 5.64e-17 74.3
MsaG025337 AT2G18060.3 56.863 51 22 0 1 51 4 54 7.70e-17 74.3
MsaG025337 AT2G18060.1 56.863 51 22 0 1 51 4 54 7.70e-17 74.3
MsaG025337 AT4G27410.3 64.706 51 18 0 5 55 13 63 1.25e-16 73.6
MsaG025337 AT1G61110.1 57.692 52 22 0 2 53 12 63 1.33e-16 73.6
MsaG025337 AT4G36160.1 60.000 50 20 0 5 54 9 58 1.40e-16 73.9
MsaG025337 AT4G36160.3 60.000 50 20 0 5 54 9 58 1.40e-16 73.9
MsaG025337 AT5G22290.1 59.615 52 21 0 1 52 16 67 1.50e-16 73.6
MsaG025337 AT4G36160.2 60.000 50 20 0 5 54 13 62 1.50e-16 73.9
MsaG025337 AT2G02450.2 60.870 46 18 0 8 53 53 98 1.56e-16 73.9
MsaG025337 AT2G02450.1 60.870 46 18 0 8 53 53 98 1.93e-16 73.6
MsaG025337 AT3G61910.1 52.000 50 24 0 2 51 7 56 2.92e-16 72.8
MsaG025337 AT4G27410.2 66.667 48 16 0 5 52 13 60 5.27e-16 71.6
MsaG025337 AT1G01720.1 62.500 48 18 0 6 53 7 54 1.16e-15 70.9
MsaG025337 AT3G44290.2 64.444 45 16 0 8 52 16 60 1.28e-15 69.7
MsaG025337 AT1G71930.2 56.250 48 21 0 4 51 7 54 1.31e-15 70.9
MsaG025337 AT1G71930.1 56.250 48 21 0 4 51 7 54 1.31e-15 70.9
MsaG025337 AT1G26870.1 59.574 47 19 0 6 52 16 62 1.34e-15 71.2
MsaG025337 AT1G69490.1 57.143 49 21 0 4 52 7 55 1.43e-15 70.1
MsaG025337 AT3G04070.1 58.000 50 21 0 4 53 8 57 2.01e-15 70.5
MsaG025337 AT3G04070.2 58.000 50 21 0 4 53 8 57 2.04e-15 70.5
MsaG025337 AT3G44290.1 64.444 45 16 0 8 52 16 60 2.06e-15 70.5
MsaG025337 AT1G12260.1 56.250 48 21 0 4 51 5 52 3.21e-15 70.1
MsaG025337 AT2G46770.1 52.000 50 24 0 2 51 12 61 3.80e-15 69.7
MsaG025337 AT3G12910.1 50.000 66 28 3 8 69 23 87 4.34e-15 69.3
MsaG025337 AT1G12260.2 56.250 48 21 0 4 51 5 52 4.38e-15 69.7
MsaG025337 AT5G39820.1 57.778 45 19 0 8 52 22 66 5.13e-15 69.3
MsaG025337 AT2G27300.1 59.184 49 20 0 4 52 12 60 5.85e-15 69.3
MsaG025337 AT5G64530.2 52.727 55 26 0 5 59 2 56 7.69e-15 66.2
MsaG025337 AT1G33060.2 57.143 49 21 0 5 53 23 71 9.27e-15 68.9
MsaG025337 AT1G33060.1 57.143 49 21 0 5 53 23 71 9.54e-15 68.9
MsaG025337 AT1G52890.1 62.500 48 18 0 5 52 13 60 1.22e-14 68.2
MsaG025337 AT3G10480.3 54.902 51 23 0 1 51 22 72 1.26e-14 68.6
MsaG025337 AT3G10480.1 54.902 51 23 0 1 51 22 72 1.32e-14 68.6
MsaG025337 AT3G10480.2 54.902 51 23 0 1 51 22 72 1.35e-14 68.6
MsaG025337 AT5G64530.1 52.727 55 26 0 5 59 2 56 1.59e-14 66.2
MsaG025337 AT1G34190.1 59.615 52 21 0 2 53 12 63 1.86e-14 68.2
MsaG025337 AT3G10490.1 57.143 49 21 0 3 51 24 72 1.87e-14 67.0
MsaG025337 AT5G13180.1 49.254 67 34 0 2 68 10 76 2.02e-14 67.0
MsaG025337 AT3G15500.1 62.500 48 18 0 5 52 13 60 2.47e-14 67.4
MsaG025337 AT5G04410.1 59.184 49 20 0 4 52 7 55 2.54e-14 67.8
MsaG025337 AT3G10490.2 57.143 49 21 0 3 51 24 72 2.67e-14 67.4
MsaG025337 AT5G64060.1 55.769 52 23 0 1 52 1 52 2.68e-14 67.4
MsaG025337 AT4G10350.1 53.704 54 22 1 1 51 1 54 2.82e-14 67.4
MsaG025337 AT3G10500.1 58.333 48 20 0 5 52 8 55 3.39e-14 67.4
MsaG025337 AT1G79580.3 58.000 50 21 0 2 51 13 62 3.91e-14 67.0
MsaG025337 AT1G79580.5 58.000 50 21 0 2 51 13 62 3.91e-14 67.0
MsaG025337 AT1G79580.4 58.000 50 21 0 2 51 13 62 3.91e-14 67.0
MsaG025337 AT1G79580.2 58.000 50 21 0 2 51 13 62 3.91e-14 67.0
MsaG025337 AT1G79580.1 58.000 50 21 0 2 51 13 62 3.91e-14 67.0
MsaG025337 AT5G08790.1 60.417 48 19 0 5 52 6 53 4.55e-14 66.2
MsaG025337 AT1G32770.1 50.000 50 25 0 2 51 12 61 6.32e-14 66.2
MsaG025337 AT5G04395.1 56.250 48 21 0 4 51 26 73 8.47e-14 64.3
MsaG025337 AT5G63790.1 58.333 48 20 0 5 52 49 96 1.06e-13 65.5
MsaG025337 AT1G62700.1 52.083 48 23 0 4 51 5 52 1.08e-13 65.9
MsaG025337 AT1G62700.2 52.083 48 23 0 4 51 5 52 1.08e-13 65.9
MsaG025337 AT5G63790.2 58.333 48 20 0 5 52 59 106 1.16e-13 65.5
MsaG025337 AT5G62380.2 52.083 48 23 0 4 51 5 52 1.67e-13 65.1
MsaG025337 AT5G62380.1 52.083 48 23 0 4 51 5 52 1.67e-13 65.1
MsaG025337 AT1G34180.2 59.574 47 19 0 8 54 18 64 2.36e-13 65.1
MsaG025337 AT1G34180.4 59.574 47 19 0 8 54 18 64 2.52e-13 64.7
MsaG025337 AT1G52880.1 56.818 44 19 0 10 53 21 64 2.69e-13 64.3
MsaG025337 AT5G09330.5 53.846 52 24 0 1 52 1 52 2.70e-13 64.7
MsaG025337 AT5G09330.2 53.846 52 24 0 1 52 1 52 2.70e-13 64.7
MsaG025337 AT5G09330.4 53.846 52 24 0 1 52 1 52 2.70e-13 64.7
MsaG025337 AT5G09330.3 53.846 52 24 0 1 52 1 52 2.70e-13 64.7
MsaG025337 AT5G09330.1 53.846 52 24 0 1 52 1 52 2.70e-13 64.7
MsaG025337 AT1G34180.1 60.870 46 18 0 8 53 18 63 3.34e-13 64.3
MsaG025337 AT1G34180.3 60.870 46 18 0 8 53 18 63 3.85e-13 64.3
MsaG025337 AT1G33280.1 56.522 46 20 0 6 51 8 53 5.36e-13 63.5
MsaG025337 AT4G35580.3 53.061 49 23 0 5 53 8 56 6.42e-13 63.5
MsaG025337 AT4G35580.2 53.061 49 23 0 5 53 8 56 6.55e-13 63.5
MsaG025337 AT3G15510.1 56.818 44 19 0 10 53 21 64 9.62e-13 63.2
MsaG025337 AT5G24590.2 55.102 49 22 0 4 52 11 59 2.25e-12 62.0
MsaG025337 AT4G35580.1 53.061 49 23 0 5 53 8 56 2.26e-12 62.0
MsaG025337 AT3G49530.1 54.167 48 22 0 5 52 12 59 2.33e-12 62.0
MsaG025337 AT1G77450.1 52.830 53 23 1 2 52 4 56 2.40e-12 61.2
MsaG025337 AT2G17040.1 56.522 46 20 0 8 53 8 53 2.99e-12 61.2
MsaG025337 AT3G49530.2 54.167 48 22 0 5 52 12 59 3.91e-12 61.2
MsaG025337 AT2G24430.2 54.167 48 22 0 5 52 15 62 4.29e-12 61.2
MsaG025337 AT2G24430.1 54.167 48 22 0 5 52 15 62 4.29e-12 61.2
MsaG025337 AT3G18400.1 51.020 49 24 0 5 53 4 52 6.15e-12 60.5
MsaG025337 AT3G15170.1 55.319 47 21 0 6 52 20 66 6.19e-12 60.5
MsaG025337 AT5G07680.2 57.447 47 20 0 6 52 3 49 6.62e-12 60.5
MsaG025337 AT5G53950.1 55.556 54 22 1 2 53 11 64 6.67e-12 60.8
MsaG025337 AT5G07680.1 57.447 47 20 0 6 52 17 63 7.04e-12 60.5
MsaG025337 AT5G61430.1 55.319 47 21 0 6 52 16 62 7.91e-12 60.5
MsaG025337 AT3G04060.1 42.424 66 36 1 6 69 20 85 3.28e-11 58.5

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCGTTCCCATTGGTTGAAA+TGG 0.287030 5:-6828287 None:intergenic
AATTCTACAAATTTGATCCT+TGG 0.356563 5:+6827082 MsaT025337.1:CDS
ATTCTACAAATTTGATCCTT+GGG 0.375015 5:+6827083 MsaT025337.1:CDS
AATTTGTAGAATTCAATAAC+AGG 0.424548 5:-6827072 None:intergenic
TGGGAACGAGGTTTCTTGTC+AGG 0.433403 5:+6828300 MsaT025337.1:CDS
TCGAAGGACAAGGAGGAAAG+AGG 0.464828 5:+6828239 MsaT025337.1:CDS
CCAACCGATGAAGAGTTGAT+AGG 0.477569 5:+6826997 MsaT025337.1:CDS
GCAATTCCATTTCAACCAAT+GGG 0.481299 5:+6828281 MsaT025337.1:CDS
AAGAGGGTTAGAGCTAATGA+TGG 0.484892 5:+6828256 MsaT025337.1:CDS
AGCAATTCCATTTCAACCAA+TGG 0.488117 5:+6828280 MsaT025337.1:CDS
GGAGGAGCTTCACTGCCTCC+AGG 0.489497 5:+6826961 MsaT025337.1:CDS
CCTATCAACTCTTCATCGGT+TGG 0.504532 5:-6826997 None:intergenic
TCAACTCTTCATCGGTTGGA+TGG 0.522689 5:-6826993 None:intergenic
ACAAGAAACCTCGTTCCCAT+TGG 0.531913 5:-6828296 None:intergenic
AGACATTTACCTCCGAGCCT+TGG 0.532346 5:-6828067 None:intergenic
TTTGATCCTTGGGAGTTACC+TGG 0.560186 5:+6827093 MsaT025337.1:CDS
GAAATTGAAGATGACCTACC+AGG 0.568955 5:-6827111 None:intergenic
ATATCCTATCAACTCTTCAT+CGG 0.573542 5:-6827001 None:intergenic
CATTTCAACCAATGGGAACG+AGG 0.580432 5:+6828288 MsaT025337.1:CDS
CGAAGGACAAGGAGGAAAGA+GGG 0.583348 5:+6828240 MsaT025337.1:CDS
GTTGGATGGAAACGAAAACC+TGG 0.583973 5:-6826979 None:intergenic
TGAGAAGGCAAATGATTCGA+AGG 0.585286 5:+6828223 MsaT025337.1:CDS
TGATGATCCTAGCCAAGGCT+CGG 0.588446 5:+6828055 MsaT025337.1:intron
ATCCTTGGGAGTTACCTGGT+AGG 0.625230 5:+6827097 MsaT025337.1:CDS
ACATAGAAATATAGAATGGG+AGG 0.627145 5:+6826943 None:intergenic
AGAGGGTTAGAGCTAATGAT+GGG 0.630099 5:+6828257 MsaT025337.1:CDS
GGCAAATGATTCGAAGGACA+AGG 0.640965 5:+6828229 MsaT025337.1:CDS
GACCTACCAGGTAACTCCCA+AGG 0.644578 5:-6827099 None:intergenic
TGATCCTAGCCAAGGCTCGG+AGG 0.696143 5:+6828058 MsaT025337.1:intron
AAATGATTCGAAGGACAAGG+AGG 0.713594 5:+6828232 MsaT025337.1:CDS
GGATGGAAACGAAAACCTGG+AGG 0.767680 5:-6826976 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 6826958 6828337 6826958 ID=MsaG025337
Chr5 mRNA 6826958 6828337 6826958 ID=MsaT025337.1;Parent=MsaG025337
Chr5 exon 6826958 6827118 6826958 ID=MsaT025337.1.exon1;Parent=MsaT025337.1
Chr5 CDS 6826958 6827118 6826958 ID=cds.MsaT025337.1;Parent=MsaT025337.1
Chr5 exon 6828067 6828079 6828067 ID=MsaT025337.1.exon2;Parent=MsaT025337.1
Chr5 CDS 6828067 6828079 6828067 ID=cds.MsaT025337.1;Parent=MsaT025337.1
Chr5 exon 6828188 6828337 6828188 ID=MsaT025337.1.exon3;Parent=MsaT025337.1
Chr5 CDS 6828188 6828337 6828188 ID=cds.MsaT025337.1;Parent=MsaT025337.1
Gene Sequence

>MsaT025337.1

ATGGGAGGAGCTTCACTGCCTCCAGGTTTTCGTTTCCATCCAACCGATGAAGAGTTGATAGGATATTACCTAAAAAGAAAAGTTGAGGGACTTGAAATTGAGCTTGAAGTTATACCTGTTATTGAATTCTACAAATTTGATCCTTGGGAGTTACCTGGTAGCCAAGGCTCGGAGGGTGCTTTTGCTTTATGCCGAGTTATTAAAAAGAATGAGAAGGCAAATGATTCGAAGGACAAGGAGGAAAGAGGGTTAGAGCTAATGATGGGAGCAATTCCATTTCAACCAATGGGAACGAGGTTTCTTGTCAGGCAAGTCAACTATTGA

Protein sequence

>MsaT025337.1

MGGASLPPGFRFHPTDEELIGYYLKRKVEGLEIELEVIPVIEFYKFDPWELPGSQGSEGAFALCRVIKKNEKANDSKDKEERGLELMMGAIPFQPMGTRFLVRQVNY*