AlfalfaGEDB | MsaG025393

M. sativa ssp. caerulea / MsaG025393

KEGG: -
GO: GO:0003002 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008356 GO:0009889 GO:0009956 GO:0009987 GO:0010468 GO:0010556 GO:0010817 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032350 GO:0032501 GO:0032502 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045786 GO:0045930 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051171 GO:0051252 GO:0051301 GO:0051726 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060255 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098771 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03514.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025393	XP_003611578.1	98.045	358	5	1	1	356	134	491	0.0	729

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025393	sp\|Q9SZF7\|SHR_ARATH	68.835	369	98	6	1	356	166	530	3.03e-180	513

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025393	tr\|G7K2I5\|G7K2I5_MEDTR	98.045	358	5	1	1	356	134	491	0.0	729

TFs/TRs:

Gene ID	Type	Classification
MsaG025393	TF	GRAS

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000479	MsaG025393	0.818196	3.876521e-52	8.132300e-50
MsaG000842	MsaG025393	0.832283	1.867398e-55	5.772828e-53
MsaG002209	MsaG025393	0.830714	4.525773e-55	1.336746e-52
MsaG002405	MsaG025393	0.831023	3.804011e-55	1.133634e-52
MsaG002484	MsaG025393	0.817214	6.441674e-52	1.317620e-49
MsaG002972	MsaG025393	0.812883	5.831640e-51	1.068993e-48
MsaG003305	MsaG025393	0.810425	1.984618e-50	3.424584e-48
MsaG004805	MsaG025393	0.849857	4.698444e-60	2.533903e-57
MsaG004820	MsaG025393	0.832597	1.562218e-55	4.873632e-53
MsaG005850	MsaG025393	0.812063	8.791669e-51	1.579034e-48
MsaG005928	MsaG025393	0.820405	1.223820e-52	2.720639e-50
MsaG006552	MsaG025393	0.836104	2.078561e-56	7.196931e-54
MsaG006925	MsaG025393	0.838677	4.592899e-57	1.720267e-54
MsaG009772	MsaG025393	0.801092	1.774751e-48	2.459397e-46
MsaG012174	MsaG025393	0.859268	9.115510e-63	6.893553e-60
MsaG015833	MsaG025393	0.847274	2.420822e-59	1.196403e-56
MsaG016119	MsaG025393	0.851901	1.255821e-60	7.270159e-58
MsaG016770	MsaG025393	0.816293	1.034024e-51	2.065541e-49
MsaG018847	MsaG025393	0.823213	2.760390e-53	6.614140e-51
MsaG019621	MsaG025393	0.802489	9.199168e-49	1.316009e-46
MsaG019629	MsaG025393	0.821139	8.309346e-53	1.883518e-50
MsaG020206	MsaG025393	0.800754	2.079515e-48	2.859914e-46
MsaG020335	MsaG025393	0.810806	1.643547e-50	2.862449e-48
MsaG022216	MsaG025393	0.801688	1.341599e-48	1.884492e-46
MsaG022478	MsaG025393	0.803788	4.969997e-49	7.325869e-47
MsaG024150	MsaG025393	0.808834	4.345651e-50	7.215383e-48
MsaG024152	MsaG025393	0.818287	3.698379e-52	7.776515e-50
MsaG025393	MsaG025404	0.843732	2.183923e-58	9.600554e-56
MsaG025393	MsaG026649	0.807168	9.792235e-50	1.562123e-47
MsaG025393	MsaG026651	0.807920	6.791128e-50	1.103090e-47
MsaG025393	MsaG026770	0.809250	3.542471e-50	5.940770e-48
MsaG025393	MsaG027631	0.804886	2.941579e-49	4.447870e-47
MsaG025393	MsaG028939	0.808623	4.818264e-50	7.959388e-48
MsaG025393	MsaG030080	0.814156	3.070504e-51	5.810678e-49
MsaG025393	MsaG034278	0.803880	4.757096e-49	7.026982e-47
MsaG025393	MsaG036511	0.812352	7.609876e-51	1.376642e-48
MsaG025393	MsaG039176	0.831998	2.194561e-55	6.728738e-53
MsaG025393	MsaG040327	0.800155	2.750293e-48	3.731538e-46
MsaG025393	MsaG041118	0.806358	1.449011e-49	2.267886e-47
MsaG025393	MsaG041277	0.827743	2.361718e-54	6.412037e-52
MsaG025393	MsaG042750	0.825195	9.493393e-54	2.400887e-51
MsaG025393	MsaG043344	0.809535	3.078980e-50	5.199536e-48
MsaG025393	MsaG045261	0.824117	1.699136e-53	4.172465e-51
MsaG025393	MsaG045766	0.803300	6.267126e-49	9.134150e-47
MsaG025393	MsaG046532	0.807867	6.971059e-50	1.130835e-47
MsaG025393	MsaG046425	0.842646	4.239615e-58	1.800030e-55
MsaG025393	MsaG046732	0.810756	1.685132e-50	2.931244e-48
MsaG025393	MsaG001553	0.817905	4.507893e-52	9.385833e-50
MsaG025393	MsaG001996	0.818304	3.666403e-52	7.712685e-50
MsaG025393	MsaG003316	0.824214	1.612629e-53	3.970381e-51
MsaG025393	MsaG007187	0.862971	6.886081e-64	6.003067e-61
MsaG025393	MsaG025840	0.809826	2.667888e-50	4.537003e-48
MsaG025393	MsaG029509	0.810796	1.651586e-50	2.875751e-48
MsaG025393	MsaG027119	0.813110	5.202423e-51	9.590560e-49
MsaG025393	MsaG027196	0.813731	3.804299e-51	7.123292e-49
MsaG025393	MsaG030868	0.814727	2.298958e-51	4.413378e-49
MsaG025393	MsaG031297	0.820410	1.220323e-52	2.713241e-50
MsaG025393	MsaG035504	0.841582	8.083076e-58	3.316834e-55
MsaG025393	MsaG041203	0.800991	1.861061e-48	2.573151e-46
MsaG025393	MsaG043311	0.860101	5.132096e-63	4.005756e-60

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025393	MtrunA17_Chr5g0401131	98.045	358	5	1	1	356	134	491	0.0	729
MsaG025393	MtrunA17_Chr2g0329591	55.676	370	139	6	1	356	94	452	3.34e-138	400
MsaG025393	MtrunA17_Chr1g0159461	31.653	357	227	8	4	355	248	592	2.95e-53	185
MsaG025393	MtrunA17_Chr2g0319091	32.571	350	217	7	12	355	243	579	2.52e-51	179
MsaG025393	MtrunA17_Chr5g0447691	29.945	364	231	8	2	355	195	544	5.19e-51	178
MsaG025393	MtrunA17_Chr5g0445331	30.685	365	231	8	2	355	179	532	7.19e-51	177
MsaG025393	MtrunA17_Chr3g0125811	31.373	357	228	8	4	355	226	570	4.73e-49	173
MsaG025393	MtrunA17_Chr3g0101141	30.435	368	224	10	2	355	193	542	1.90e-48	171
MsaG025393	MtrunA17_Chr2g0290241	29.444	360	233	8	3	355	80	425	6.10e-48	167
MsaG025393	MtrunA17_Chr4g0053901	29.040	396	236	9	1	356	79	469	4.69e-46	163
MsaG025393	MtrunA17_Chr7g0224271	31.200	375	217	11	1	355	320	673	2.57e-45	164
MsaG025393	MtrunA17_Chr4g0054071	79.070	86	18	0	1	86	153	238	3.51e-44	152
MsaG025393	MtrunA17_Chr4g0076091	29.752	363	231	9	2	354	194	542	2.15e-43	157
MsaG025393	MtrunA17_Chr5g0401421	28.040	403	233	11	1	356	55	447	3.37e-42	152
MsaG025393	MtrunA17_Chr8g0376381	32.667	300	189	6	59	355	233	522	3.70e-42	154
MsaG025393	MtrunA17_Chr8g0357601	30.141	355	232	9	7	355	208	552	1.14e-40	150
MsaG025393	MtrunA17_Chr2g0306641	30.205	341	220	7	17	355	163	487	1.23e-40	149
MsaG025393	MtrunA17_Chr4g0057291	28.729	362	236	11	4	356	315	663	1.66e-40	151
MsaG025393	MtrunA17_Chr3g0110971	29.096	354	217	6	16	355	254	587	6.10e-40	149
MsaG025393	MtrunA17_Chr4g0031741	29.834	362	234	9	1	356	304	651	5.92e-39	146
MsaG025393	MtrunA17_Chr7g0245601	29.330	358	223	13	4	356	461	793	1.31e-38	146
MsaG025393	MtrunA17_Chr8g0344101	30.688	378	219	13	1	355	243	600	1.66e-37	142
MsaG025393	MtrunA17_Chr4g0039661	30.579	363	218	11	4	355	216	555	2.01e-37	141
MsaG025393	MtrunA17_Chr4g0038761	28.771	358	229	13	4	356	107	443	2.16e-37	139
MsaG025393	MtrunA17_Chr5g0396491	26.392	413	230	9	4	355	79	478	9.35e-37	138
MsaG025393	MtrunA17_Chr4g0031711	27.298	359	253	4	1	356	381	734	1.07e-34	134
MsaG025393	MtrunA17_Chr2g0328391	29.577	355	234	7	8	356	326	670	1.08e-34	134
MsaG025393	MtrunA17_Chr2g0328341	27.147	361	244	10	3	356	291	639	4.61e-34	132
MsaG025393	MtrunA17_Chr4g0031701	28.746	327	221	7	4	325	277	596	8.64e-34	131
MsaG025393	MtrunA17_Chr4g0031721	27.577	359	248	5	1	356	336	685	2.54e-33	130
MsaG025393	MtrunA17_Chr4g0057891	26.757	370	237	11	3	355	168	520	6.68e-33	128
MsaG025393	MtrunA17_Chr4g0031731	28.746	327	221	7	4	325	277	596	2.14e-32	127
MsaG025393	MtrunA17_Chr7g0238891	25.348	359	256	5	1	356	392	741	7.40e-32	126
MsaG025393	MtrunA17_Chr2g0328381	26.558	369	236	10	3	356	294	642	1.19e-31	125
MsaG025393	MtrunA17_Chr2g0328411	25.915	355	248	6	3	354	311	653	2.57e-31	124
MsaG025393	MtrunA17_Chr3g0085681	23.821	403	242	9	4	355	48	436	8.16e-31	121
MsaG025393	MtrunA17_Chr2g0328431	26.479	355	240	7	8	356	316	655	3.44e-30	121
MsaG025393	MtrunA17_Chr2g0328401	27.640	322	214	7	8	324	317	624	3.52e-28	115
MsaG025393	MtrunA17_Chr3g0114841	28.496	379	219	14	4	354	145	499	1.32e-27	113
MsaG025393	MtrunA17_Chr1g0183641	26.891	357	229	13	4	354	146	476	5.89e-26	108
MsaG025393	MtrunA17_Chr1g0200401	26.738	374	219	13	12	355	111	459	6.18e-25	105
MsaG025393	MtrunA17_Chr4g0038851	32.338	201	126	4	7	206	74	265	6.05e-24	102
MsaG025393	MtrunA17_Chr2g0328361	24.234	359	232	9	3	356	293	616	8.53e-24	102
MsaG025393	MtrunA17_Chr3g0085361	22.899	345	204	7	61	355	18	350	4.38e-23	98.6
MsaG025393	MtrunA17_Chr2g0323111	32.967	182	116	4	176	356	131	307	4.78e-23	97.8
MsaG025393	MtrunA17_Chr8g0383981	26.781	351	216	16	17	356	177	497	9.21e-23	99.4
MsaG025393	MtrunA17_Chr2g0294471	25.683	366	242	12	4	355	227	576	1.28e-22	99.0
MsaG025393	MtrunA17_Chr5g0424131	27.596	366	230	11	7	354	150	498	1.35e-22	98.6
MsaG025393	MtrunA17_Chr7g0236851	26.829	287	196	6	73	354	379	656	3.82e-21	94.7
MsaG025393	MtrunA17_Chr1g0194621	25.208	361	249	10	3	355	171	518	3.85e-20	91.7
MsaG025393	MtrunA17_Chr2g0328371	26.104	249	174	6	61	307	8	248	5.43e-20	88.2
MsaG025393	MtrunA17_Chr2g0294461	23.770	366	258	8	1	355	230	585	6.27e-20	91.3
MsaG025393	MtrunA17_Chr7g0270031	24.286	350	252	6	8	355	216	554	4.42e-18	85.5
MsaG025393	MtrunA17_Chr6g0468561	25.895	363	234	11	4	355	324	662	4.74e-17	82.4
MsaG025393	MtrunA17_Chr4g0012071	21.229	358	251	9	4	355	219	551	7.13e-14	72.8
MsaG025393	MtrunA17_Chr7g0230771	21.563	371	235	9	4	355	398	731	4.61e-12	67.4
MsaG025393	MtrunA17_Chr7g0230731	21.294	371	236	9	4	355	395	728	5.12e-12	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025393	AT4G37650.1	68.835	369	98	6	1	356	166	530	0.0	513
MsaG025393	AT2G04890.1	31.844	358	225	8	2	355	71	413	3.55e-57	191
MsaG025393	AT1G21450.1	32.877	365	222	8	1	355	242	593	3.56e-50	177
MsaG025393	AT5G48150.1	29.050	358	242	6	1	355	142	490	8.32e-49	171
MsaG025393	AT5G48150.4	29.050	358	242	6	1	355	142	490	8.32e-49	171
MsaG025393	AT5G48150.2	29.050	358	242	6	1	355	142	490	8.32e-49	171
MsaG025393	AT5G48150.3	29.050	358	242	6	1	355	142	490	8.32e-49	171
MsaG025393	AT1G50600.3	30.874	366	225	10	2	355	177	526	1.95e-48	171
MsaG025393	AT3G49950.1	32.162	370	227	9	1	356	50	409	2.65e-48	168
MsaG025393	AT1G50600.1	30.874	366	225	10	2	355	248	597	4.80e-48	171
MsaG025393	AT1G50600.2	30.874	366	225	10	2	355	252	601	5.32e-48	171
MsaG025393	AT3G03450.1	31.487	343	215	7	17	355	219	545	3.35e-47	168
MsaG025393	AT1G66350.1	33.239	352	206	11	7	355	181	506	9.98e-47	166
MsaG025393	AT5G17490.1	33.138	341	206	8	17	355	196	516	1.13e-46	166
MsaG025393	AT2G01570.1	30.029	343	217	10	16	355	259	581	3.46e-40	149
MsaG025393	AT1G14920.1	30.233	344	215	10	16	355	207	529	2.49e-39	146
MsaG025393	AT1G50420.1	25.854	410	236	6	4	355	80	479	2.36e-38	143
MsaG025393	AT4G17230.1	26.316	361	245	8	3	356	180	526	1.26e-37	142
MsaG025393	AT4G17230.2	26.316	361	245	8	3	356	180	526	1.26e-37	142
MsaG025393	AT3G54220.1	29.363	361	222	14	4	356	316	651	1.66e-36	140
MsaG025393	AT1G63100.2	29.863	365	227	11	6	354	302	653	1.96e-35	137
MsaG025393	AT1G63100.1	29.863	365	227	11	6	354	277	628	2.48e-35	136
MsaG025393	AT5G41920.1	29.805	359	221	14	4	355	66	400	2.83e-34	130
MsaG025393	AT1G55580.1	28.198	383	228	11	10	355	73	445	2.49e-33	129
MsaG025393	AT5G66770.1	28.846	364	232	10	1	355	239	584	2.82e-33	130
MsaG025393	AT2G29065.1	26.575	365	252	9	1	356	272	629	4.14e-32	127
MsaG025393	AT3G46600.2	27.731	357	243	8	4	356	27	372	1.61e-31	122
MsaG025393	AT3G13840.1	29.539	369	216	13	3	355	170	510	2.07e-31	124
MsaG025393	AT5G52510.1	28.412	359	219	9	15	355	302	640	4.70e-31	124
MsaG025393	AT1G07530.1	26.331	357	250	6	4	356	419	766	4.94e-31	124
MsaG025393	AT2G37650.1	26.705	352	244	6	8	356	374	714	5.78e-31	124
MsaG025393	AT2G37650.2	26.705	352	244	6	8	356	362	702	6.34e-31	124
MsaG025393	AT3G46600.3	27.731	357	243	8	4	356	203	548	3.08e-30	121
MsaG025393	AT3G46600.1	27.731	357	243	8	4	356	235	580	6.12e-30	120
MsaG025393	AT3G50650.1	29.640	361	213	11	12	355	206	542	6.25e-29	117
MsaG025393	AT1G07520.2	25.989	354	251	7	9	356	182	530	9.84e-29	117
MsaG025393	AT1G07520.4	25.989	354	251	7	9	356	182	530	9.84e-29	117
MsaG025393	AT1G07520.1	25.989	354	251	7	9	356	346	694	2.27e-28	116
MsaG025393	AT1G07520.3	25.989	354	251	7	9	356	347	695	2.52e-28	116
MsaG025393	AT2G29060.2	25.627	359	252	8	4	356	307	656	3.30e-28	116
MsaG025393	AT2G29060.1	25.627	359	252	8	4	356	344	693	4.03e-28	115
MsaG025393	AT5G59450.1	26.836	354	246	6	8	356	254	599	1.07e-27	114
MsaG025393	AT4G08250.1	27.759	299	202	9	61	354	187	476	1.06e-23	102
MsaG025393	AT4G00150.1	23.514	370	217	13	5	355	232	554	3.36e-12	68.2
MsaG025393	AT3G60630.1	24.919	309	199	12	61	355	333	622	3.56e-12	68.2

Find 89 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGGAGGCTGATTTAAATTT+TGG	0.142361	5:-7763278	MsaT025393.1:CDS
TGAATAACGTTGAATTTAAA+TGG	0.226774	5:+7763488	None:intergenic
AAGAGTGTTTGAGATGGTTT+AGG	0.270052	5:-7763223	MsaT025393.1:CDS
GGCTTTGTTGAGGAGGTATA+AGG	0.272310	5:-7762985	MsaT025393.1:CDS
TTCGGTCATTCGAGAGAATA+AGG	0.284815	5:+7763868	None:intergenic
ACCTTCGAAAGCTTCTAAAA+TGG	0.330622	5:+7763709	None:intergenic
TGGGAGAGGGATAGTTGATT+TGG	0.342461	5:-7763129	MsaT025393.1:CDS
AGGAAGTTTGTGATGATGTT+AGG	0.369294	5:-7763007	MsaT025393.1:CDS
GAAGGTTCTGGTACCGGCTT+CGG	0.378697	5:+7763850	None:intergenic
AGAGTGTTTGAGATGGTTTA+GGG	0.380120	5:-7763222	MsaT025393.1:CDS
TAGTCCATGGACTAACTTCT+TGG	0.385254	5:+7763759	None:intergenic
CGTTGATCGCGTTGCGGCCG+AGG	0.391480	5:-7763319	MsaT025393.1:CDS
CTTCCACGTCAAGAATATTC+CGG	0.409628	5:+7762932	None:intergenic
GTTAGTCCATGGACTACTTT+CGG	0.413327	5:-7763752	MsaT025393.1:CDS
AGCTAGGGTTCTGAAGGTTC+TGG	0.415747	5:+7763838	None:intergenic
GAAGGAAATTGGTGCTAGAA+TGG	0.434419	5:-7763537	MsaT025393.1:CDS
ATGATACGGTGTAATGTTGC+CGG	0.444868	5:-7762951	MsaT025393.1:CDS
AACGAACTAAGCTCCCCTTA+TGG	0.445036	5:-7763929	MsaT025393.1:CDS
TTCTCTCGAATGACCGAAGC+CGG	0.449633	5:-7763863	MsaT025393.1:CDS
TCACGGCGTTTGCATGGTGG+AGG	0.454306	5:-7763053	MsaT025393.1:CDS
TTGATGCTTGAAAGGGAAGC+TGG	0.455749	5:-7763149	MsaT025393.1:CDS
CAGTGACGATGGTGGTTAAA+CGG	0.455896	5:+7763585	None:intergenic
ATGTTAGGGCTTTGTTGAGG+AGG	0.458311	5:-7762992	MsaT025393.1:CDS
TTCGGCCATGTTGCATGCAA+TGG	0.459991	5:-7763734	MsaT025393.1:CDS
TTTGTTCTATGTCACCATAA+GGG	0.462505	5:+7763915	None:intergenic
CGGTGGTGTGGGCCAGTGCA+TGG	0.470323	5:-7762902	MsaT025393.1:CDS
CATTCAGTAACAGTTGGTAA+TGG	0.475130	5:-7763377	MsaT025393.1:CDS
CATGCACTGGCCCACACCAC+CGG	0.476107	5:+7762903	None:intergenic
GGAAGTTTGTGATGATGTTA+GGG	0.478484	5:-7763006	MsaT025393.1:CDS
CACGGCGTTTGCATGGTGGA+GGG	0.480736	5:-7763052	MsaT025393.1:CDS
GGTTCTGAAGGTTCTGGTAC+CGG	0.480940	5:+7763844	None:intergenic
AAACGCTCGTTACTCGTCTT+CGG	0.484291	5:+7763170	None:intergenic
ATGGTTTAGGGTTTACTTTG+AGG	0.492134	5:-7763210	MsaT025393.1:CDS
GATGGATATGAATTTGTGAA+TGG	0.492688	5:-7763251	MsaT025393.1:CDS
GTAACAGTTGGTAATGGTAA+TGG	0.498845	5:-7763371	MsaT025393.1:CDS
GCATGGTGGAGGGTTTAATA+CGG	0.512491	5:-7763042	MsaT025393.1:CDS
CGGAATATTCTTGACGTGGA+AGG	0.512741	5:-7762931	MsaT025393.1:CDS
TGATTTGGTGGCATGTGCTC+CGG	0.516469	5:-7763114	MsaT025393.1:CDS
TTATCAAGGCCTCCATGCAC+TGG	0.517596	5:+7762890	None:intergenic
GGAAGGAACAACCGGTGGTG+TGG	0.520705	5:-7762914	MsaT025393.1:CDS
GAGAGGGATAGTTGATTTGG+TGG	0.522738	5:-7763126	MsaT025393.1:CDS
CTTGACGTGGAAGGAACAAC+CGG	0.523785	5:-7762922	MsaT025393.1:CDS
AGGAAGGGTGGTCGATGATA+CGG	0.526878	5:-7762965	MsaT025393.1:CDS
CTCCGTTCAATCGACTCTGC+CGG	0.527978	5:+7763095	None:intergenic
ACGAGCGTTTGATGCTTGAA+AGG	0.528089	5:-7763157	MsaT025393.1:CDS
ATTCAACGTTATTCATCACT+CGG	0.529452	5:-7763480	MsaT025393.1:CDS
GAAGGAACAACCGGTGGTGT+GGG	0.535300	5:-7762913	MsaT025393.1:CDS
GTCGATTGAACGGAGAGAAA+CGG	0.535584	5:-7763087	MsaT025393.1:CDS
TCAACCACTGTTACTATCCT+CGG	0.540676	5:+7763302	None:intergenic
TTGTTCTATGTCACCATAAG+GGG	0.542773	5:+7763916	None:intergenic
CTTGAAAGGGAAGCTGGGAG+AGG	0.543069	5:-7763143	MsaT025393.1:CDS
CGAGCGTTTGATGCTTGAAA+GGG	0.543796	5:-7763156	MsaT025393.1:CDS
GGAGAGAAACGGCGGTGAGA+TGG	0.549109	5:-7763076	MsaT025393.1:CDS
TTAACCACCATCGTCACTGC+TGG	0.551969	5:-7763581	MsaT025393.1:CDS
GATAGTAACAGTGGTTGAAG+AGG	0.553960	5:-7763297	MsaT025393.1:CDS
TGCTGAAGCTAGGGTTCTGA+AGG	0.554762	5:+7763832	None:intergenic
GCTTTGCATTCAGTAACAGT+TGG	0.557029	5:-7763383	MsaT025393.1:CDS
AGATGGTCACGGCGTTTGCA+TGG	0.559260	5:-7763059	MsaT025393.1:CDS
AATTCCAAGAAGTTAGTCCA+TGG	0.567956	5:-7763763	MsaT025393.1:CDS
ACCACCATCGTCACTGCTGG+TGG	0.581472	5:-7763578	MsaT025393.1:CDS
AGTAACAGTGGTTGAAGAGG+AGG	0.584206	5:-7763294	MsaT025393.1:CDS
CTCCGGCAGAGTCGATTGAA+CGG	0.585476	5:-7763097	MsaT025393.1:CDS
TGATGCTTGAAAGGGAAGCT+GGG	0.585745	5:-7763148	MsaT025393.1:CDS
TGTTGAGGAGGTATAAGGAA+GGG	0.588341	5:-7762980	MsaT025393.1:CDS
TTGTTGAGGAGGTATAAGGA+AGG	0.589974	5:-7762981	MsaT025393.1:CDS
CGGAGTATCATCAGCACGAG+TGG	0.595472	5:+7763613	None:intergenic
TCATTCGAGAGAATAAGGCT+TGG	0.601622	5:+7763873	None:intergenic
TTGAAAGGGAAGCTGGGAGA+GGG	0.603420	5:-7763142	MsaT025393.1:CDS
TTCAACGTTATTCATCACTC+GGG	0.607328	5:-7763479	MsaT025393.1:CDS
ATGATGTTAGGGCTTTGTTG+AGG	0.607674	5:-7762995	MsaT025393.1:CDS
ACATGGCCGAAAGTAGTCCA+TGG	0.613056	5:+7763746	None:intergenic
AAGCTATCAACGCGTCGCGA+CGG	0.617261	5:+7763342	None:intergenic
TGGCGCCATTGCATGCAACA+TGG	0.621243	5:+7763729	None:intergenic
TGAGGAGGTATAAGGAAGGG+TGG	0.622191	5:-7762977	MsaT025393.1:CDS
TAGCTTCGTTGATCGCGTTG+CGG	0.632402	5:-7763325	MsaT025393.1:CDS
TTGCCGGAATATTCTTGACG+TGG	0.639599	5:-7762935	MsaT025393.1:CDS
TGGTCACGGCGTTTGCATGG+TGG	0.640251	5:-7763056	MsaT025393.1:CDS
AAACGGCGGTGAGATGGTCA+CGG	0.645102	5:-7763070	MsaT025393.1:CDS
TAATACGGTGGCGTTTAGTG+AGG	0.648084	5:-7763027	MsaT025393.1:CDS
AGAACCACCAGCAGTGACGA+TGG	0.652328	5:+7763574	None:intergenic
TGGTGGAGGGTTTAATACGG+TGG	0.662791	5:-7763039	MsaT025393.1:CDS
GTAACACTTATTGCACTCAG+TGG	0.674128	5:-7763658	MsaT025393.1:CDS
ATGGTGGTTAAACGGAGGTG+CGG	0.674756	5:+7763593	None:intergenic
TGGTGTGGGCCAGTGCATGG+AGG	0.695562	5:-7762899	MsaT025393.1:CDS
GACGTGGAAGGAACAACCGG+TGG	0.699506	5:-7762919	MsaT025393.1:CDS
GCGGCCGAGGATAGTAACAG+TGG	0.704672	5:-7763306	MsaT025393.1:CDS
ACCACCAGCAGTGACGATGG+TGG	0.722652	5:+7763577	None:intergenic
GATTGAACGGAGAGAAACGG+CGG	0.736491	5:-7763084	MsaT025393.1:CDS
TGACGATGGTGGTTAAACGG+AGG	0.736521	5:+7763588	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	7762893	7763963	7762893	ID=MsaG025393
Chr5	mRNA	7762893	7763963	7762893	ID=MsaT025393.1;Parent=MsaG025393
Chr5	exon	7762893	7763963	7762893	ID=MsaT025393.1.exon1;Parent=MsaT025393.1
Chr5	CDS	7762893	7763963	7762893	ID=cds.MsaT025393.1;Parent=MsaT025393.1

Gene Sequence

>MsaT025393.1

ATGTGGATGCTCAACGAACTAAGCTCCCCTTATGGTGACATAGAACAAAAACTAGCTGCATATTTTCTCCAAGCCTTATTCTCTCGAATGACCGAAGCCGGTACCAGAACCTTCAGAACCCTAGCTTCAGCATCAGAAAAAACTTGCTCATTCGAATCAACACGAAAAACAGTCCTAAAATTCCAAGAAGTTAGTCCATGGACTACTTTCGGCCATGTTGCATGCAATGGCGCCATTTTAGAAGCTTTCGAAGGTGATTCTAAGTTGCACATAATTGATATTAGTAACACTTATTGCACTCAGTGGCCAACGCTTTTTGAAGCGTTGGCCACTCGTGCTGATGATACTCCGCACCTCCGTTTAACCACCATCGTCACTGCTGGTGGTTCTGTTCAAAAAGTTATGAAGGAAATTGGTGCTAGAATGGAAAAATTCGCTAGACTTATGGGAGTTCCATTTAAATTCAACGTTATTCATCACTCGGGCGATCTTTCTGATTTAAATTTTTTGGATTTAGATATTAAAGAAGATGAAGCTTTAGCTGTTAATTGTGTTAATGCTTTGCATTCAGTAACAGTTGGTAATGGTAATGGTAATAACCGTCGCGACGCGTTGATAGCTTCGTTGATCGCGTTGCGGCCGAGGATAGTAACAGTGGTTGAAGAGGAGGCTGATTTAAATTTTGGTAATGATGGATATGAATTTGTGAATGGTTTTGAAGAGTGTTTGAGATGGTTTAGGGTTTACTTTGAGGCATTAGAAGAGAGTTTTCCGAAGACGAGTAACGAGCGTTTGATGCTTGAAAGGGAAGCTGGGAGAGGGATAGTTGATTTGGTGGCATGTGCTCCGGCAGAGTCGATTGAACGGAGAGAAACGGCGGTGAGATGGTCACGGCGTTTGCATGGTGGAGGGTTTAATACGGTGGCGTTTAGTGAGGAAGTTTGTGATGATGTTAGGGCTTTGTTGAGGAGGTATAAGGAAGGGTGGTCGATGATACGGTGTAATGTTGCCGGAATATTCTTGACGTGGAAGGAACAACCGGTGGTGTGGGCCAGTGCATGGAGGCCTTGA

Protein sequence

>MsaT025393.1

MWMLNELSSPYGDIEQKLAAYFLQALFSRMTEAGTRTFRTLASASEKTCSFESTRKTVLKFQEVSPWTTFGHVACNGAILEAFEGDSKLHIIDISNTYCTQWPTLFEALATRADDTPHLRLTTIVTAGGSVQKVMKEIGARMEKFARLMGVPFKFNVIHHSGDLSDLNFLDLDIKEDEALAVNCVNALHSVTVGNGNGNNRRDALIASLIALRPRIVTVVEEEADLNFGNDGYEFVNGFEECLRWFRVYFEALEESFPKTSNERLMLEREAGRGIVDLVACAPAESIERRETAVRWSRRLHGGGFNTVAFSEEVCDDVRALLRRYKEGWSMIRCNVAGIFLTWKEQPVVWASAWRP*