AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG025403


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025403 MtrunA17_Chr5g0401281 97.554 368 9 0 3 370 8 375 0.0 753
MsaG025403 MtrunA17_Chr4g0058071 44.781 297 159 5 63 355 17 312 5.75e-83 256
MsaG025403 MtrunA17_Chr3g0116501 38.725 204 124 1 131 334 1 203 1.46e-50 168
MsaG025403 MtrunA17_Chr4g0000031 34.426 122 66 4 243 364 1598 1705 3.63e-15 77.4
MsaG025403 MtrunA17_Chr3g0125321 29.412 153 101 3 76 226 97 244 5.51e-13 68.2
MsaG025403 MtrunA17_Chr5g0438991 38.356 73 44 1 273 345 886 957 1.30e-12 69.3
MsaG025403 MtrunA17_Chr3g0138411 39.506 81 47 2 274 353 1119 1198 4.42e-12 67.8
MsaG025403 MtrunA17_Chr6g0456861 38.889 72 43 1 274 345 692 762 6.86e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025403 AT5G67480.1 65.000 360 114 2 11 369 20 368 9.68e-178 498
MsaG025403 AT5G67480.3 65.000 360 114 2 11 369 20 368 9.68e-178 498
MsaG025403 AT5G67480.2 65.000 360 114 2 11 369 31 379 1.58e-177 498
MsaG025403 AT4G37610.1 60.976 328 126 2 41 367 34 360 8.77e-150 427
MsaG025403 AT1G05690.1 49.861 361 167 4 4 360 1 351 1.62e-110 327
MsaG025403 AT3G48360.1 43.789 322 163 8 56 361 28 347 2.28e-79 248
MsaG025403 AT5G63160.1 43.770 313 163 7 61 361 24 335 8.93e-76 239
MsaG025403 AT3G48360.2 45.862 290 142 6 56 331 28 316 1.60e-73 232
MsaG025403 AT5G63160.2 46.099 282 140 6 61 331 24 304 2.72e-71 225
MsaG025403 AT5G63160.3 45.020 251 127 7 120 361 2 250 8.63e-60 194
MsaG025403 AT3G48360.3 44.033 243 122 7 131 361 1 241 1.34e-58 191
MsaG025403 AT1G79000.3 29.577 142 82 4 223 364 1546 1669 6.49e-15 77.0
MsaG025403 AT1G79000.2 29.577 142 82 4 223 364 1570 1693 6.53e-15 77.0
MsaG025403 AT1G79000.1 29.577 142 82 4 223 364 1570 1693 6.56e-15 77.0
MsaG025403 AT1G16710.2 28.873 142 83 4 223 364 1550 1673 9.89e-13 70.5
MsaG025403 AT1G16710.3 28.873 142 83 4 223 364 1553 1676 1.01e-12 70.5
MsaG025403 AT1G16710.10 28.873 142 83 4 223 364 1553 1676 1.01e-12 70.5
MsaG025403 AT1G16710.8 28.873 142 83 4 223 364 1553 1676 1.01e-12 70.5
MsaG025403 AT1G16710.7 28.873 142 83 4 223 364 1553 1676 1.01e-12 70.5
MsaG025403 AT1G16710.6 28.873 142 83 4 223 364 1514 1637 1.04e-12 70.1
MsaG025403 AT1G16710.4 28.873 142 83 4 223 364 1514 1637 1.04e-12 70.1
MsaG025403 AT1G16710.5 28.873 142 83 4 223 364 1514 1637 1.04e-12 70.1
MsaG025403 AT1G16710.1 28.873 142 83 4 223 364 1579 1702 1.08e-12 70.1
MsaG025403 AT3G12980.1 36.471 85 50 2 276 360 1581 1661 4.84e-11 65.1
MsaG025403 AT3G12980.2 36.471 85 50 2 276 360 1500 1580 4.84e-11 65.1

Find 74 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAATTAGATGGACAATTT+TGG 0.186306 5:+7911180 MsaT025403.1:CDS
GGCGATCGATCTCAATCTTT+GGG 0.258443 5:+7909810 MsaT025403.1:CDS
TGGCGATCGATCTCAATCTT+TGG 0.276993 5:+7909809 MsaT025403.1:CDS
TTCGATATTTGTATTCTTCC+TGG 0.281886 5:+7909861 MsaT025403.1:CDS
ATCTTGAAAACAAGAGGAAT+TGG 0.329795 5:+7911211 MsaT025403.1:CDS
AAGCAATTCAAGCCCTTTGC+AGG 0.347578 5:-7910900 None:intergenic
TTGGGATTGCACCATGCTTT+CGG 0.361459 5:+7911230 MsaT025403.1:CDS
AAAGAACTTCAAAACTGTTT+CGG 0.374386 5:+7910521 MsaT025403.1:CDS
AGTTCTAGAAAAGGAAATTC+TGG 0.390373 5:+7910581 MsaT025403.1:CDS
CGCATACTCGCAAGGATAAC+CGG 0.402463 5:-7909198 None:intergenic
GCAAGAAAGACGAAATTAGA+TGG 0.406485 5:+7911170 MsaT025403.1:CDS
AAAGGAACTCTACAGAAATC+TGG 0.408140 5:-7911025 None:intergenic
ATGTGAACAGAAGCTAGAAT+TGG 0.410891 5:+7910404 MsaT025403.1:CDS
AGAGATGGTTGTCGAACTAT+TGG 0.415706 5:+7910830 MsaT025403.1:CDS
GGTTGCAAGTTGAGAGTCCC+TGG 0.418562 5:+7910938 MsaT025403.1:CDS
CTAGTTCACATTTGTAGAGA+TGG 0.440223 5:+7910815 MsaT025403.1:CDS
CGAGGCGAAGGAGGTGGTAT+CGG 0.446905 5:-7909164 None:intergenic
TCTGTAGAGTTCCTTTGTGC+AGG 0.450851 5:+7911032 MsaT025403.1:CDS
TCTTGAAAACAAGAGGAATT+GGG 0.451569 5:+7911212 MsaT025403.1:CDS
TTCAGCATGCCTCTCAATAC+AGG 0.459183 5:-7909764 None:intergenic
TGAGATCGATCGCCACCGAT+TGG 0.468351 5:-7909801 None:intergenic
GGAATTTGTCCTACATTTGT+TGG 0.476702 5:+7910341 MsaT025403.1:CDS
GAGTCACCCAGTTCTAGAAA+AGG 0.485308 5:+7910572 MsaT025403.1:CDS
GTTTGCATTAATACTGATAA+CGG 0.495992 5:+7909320 MsaT025403.1:CDS
TGTGAACAGAAGCTAGAATT+GGG 0.501782 5:+7910405 MsaT025403.1:CDS
ACACAGCTGCTTCGCCCTCT+CGG 0.509722 5:-7909239 None:intergenic
TTACGAGAAAGAAGAAATGA+AGG 0.513436 5:+7910320 MsaT025403.1:CDS
TTCTGCAACAAGAGATCTGT+GGG 0.515476 5:+7909265 MsaT025403.1:CDS
TCGCCTCGATCAGCAGCAAC+CGG 0.515743 5:+7909179 MsaT025403.1:CDS
GTTGCAAGTTGAGAGTCCCT+GGG 0.520761 5:+7910939 MsaT025403.1:CDS
CACATTCACGCTTCAAGTGA+GGG 0.529306 5:-7910386 None:intergenic
GTGCTCATTGCAAGAGGATG+TGG 0.529329 5:+7910966 MsaT025403.1:CDS
ATAAGCTGATGGCTAACATT+CGG 0.529455 5:+7909123 None:intergenic
CAAGAGGATGTGGCAACTGT+TGG 0.537744 5:+7910976 MsaT025403.1:CDS
ATTGATTGATCAGAGTAAGA+AGG 0.539090 5:+7910730 MsaT025403.1:intron
CTTTGAAGTCTTTATCATGC+GGG 0.542449 5:-7910853 None:intergenic
AAAACTGTTTCGGAGTCAGA+AGG 0.543714 5:+7910531 MsaT025403.1:CDS
GAGAAAGCACCAACAAATGT+AGG 0.546221 5:-7910350 None:intergenic
AGCAAGCAAATCGTTCCAAT+CGG 0.549306 5:+7909786 MsaT025403.1:CDS
GTTGCTGCTGATCGAGGCGA+AGG 0.550246 5:-7909176 None:intergenic
TTTGCAATTATACGATGCAA+TGG 0.552736 5:+7910787 MsaT025403.1:CDS
GTAGGTATACTTCCTGCAAA+GGG 0.554458 5:+7910888 MsaT025403.1:CDS
GCTGATCGAGGCGAAGGAGG+TGG 0.554736 5:-7909170 None:intergenic
ATCAGTATTAATGCAAACAT+CGG 0.559796 5:-7909315 None:intergenic
AGGAAGTATACCTACACGGC+TGG 0.561333 5:-7910880 None:intergenic
GAAAAGATCTTGAAAACAAG+AGG 0.566400 5:+7911205 MsaT025403.1:CDS
GCTTTGAAGTCTTTATCATG+CGG 0.567781 5:-7910854 None:intergenic
TTCATCGCCGCATACTCGCA+AGG 0.575928 5:-7909206 None:intergenic
ATGAATAATTTATCACCGAG+AGG 0.575964 5:+7909224 MsaT025403.1:CDS
GCTGCTGATCGAGGCGAAGG+AGG 0.588376 5:-7909173 None:intergenic
TGTAGGTATACTTCCTGCAA+AGG 0.593266 5:+7910887 MsaT025403.1:CDS
TTGATCAGAGTAAGAAGGAG+AGG 0.595421 5:+7910735 MsaT025403.1:intron
TCACATTCACGCTTCAAGTG+AGG 0.596344 5:-7910387 None:intergenic
CGGTTATCCTTGCGAGTATG+CGG 0.602040 5:+7909199 MsaT025403.1:CDS
CTGTTTCGGAGTCAGAAGGA+TGG 0.605228 5:+7910535 MsaT025403.1:CDS
AAGCAAATCGTTCCAATCGG+TGG 0.607764 5:+7909789 MsaT025403.1:CDS
TCAAAGCAAACCAGCCGTGT+AGG 0.611970 5:+7910870 MsaT025403.1:CDS
TGTCATTGTGGCCGAAAGCA+TGG 0.612906 5:-7911241 None:intergenic
TAACCGGTTGCTGCTGATCG+AGG 0.614451 5:-7909182 None:intergenic
GGGGTTGTGCTCATTGCAAG+AGG 0.622565 5:+7910960 MsaT025403.1:CDS
AAAACTCGTACTGCATCATG+TGG 0.623793 5:-7909836 None:intergenic
GTGGCTTCTCCTGTATTGAG+AGG 0.626268 5:+7909755 MsaT025403.1:CDS
TACATCAACTAGATTATCCA+CGG 0.627170 5:-7910436 None:intergenic
GCGATCGATCTCAATCTTTG+GGG 0.627705 5:+7909811 MsaT025403.1:CDS
CTTCTGCAACAAGAGATCTG+TGG 0.636785 5:+7909264 MsaT025403.1:CDS
TGAATAATTTATCACCGAGA+GGG 0.647099 5:+7909225 MsaT025403.1:CDS
TGCATTAATACTGATAACGG+TGG 0.661014 5:+7909323 MsaT025403.1:CDS
TTGCAAGTTGAGAGTCCCTG+GGG 0.661286 5:+7910940 MsaT025403.1:CDS
TAATATTGTTACCTGCACAA+AGG 0.667930 5:-7911043 None:intergenic
AGAATTGGGTTTACTCACCG+TGG 0.691467 5:+7910419 MsaT025403.1:CDS
TTGCAGGAAGTATACCTACA+CGG 0.699708 5:-7910884 None:intergenic
TGCAAGTTGAGAGTCCCTGG+GGG 0.717689 5:+7910941 MsaT025403.1:CDS
CATTACTGTGTGATGCTCCG+CGG 0.722450 5:+7910469 MsaT025403.1:CDS
TGACAAATGAGACTGAGCCG+CGG 0.747022 5:-7910486 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 7909131 7911261 7909131 ID=MsaG025403
Chr5 mRNA 7909131 7911261 7909131 ID=MsaT025403.1;Parent=MsaG025403
Chr5 exon 7909131 7909373 7909131 ID=MsaT025403.1.exon1;Parent=MsaT025403.1
Chr5 CDS 7909131 7909373 7909131 ID=cds.MsaT025403.1;Parent=MsaT025403.1
Chr5 exon 7909752 7909882 7909752 ID=MsaT025403.1.exon2;Parent=MsaT025403.1
Chr5 CDS 7909752 7909882 7909752 ID=cds.MsaT025403.1;Parent=MsaT025403.1
Chr5 exon 7910320 7910626 7910320 ID=MsaT025403.1.exon3;Parent=MsaT025403.1
Chr5 CDS 7910320 7910626 7910320 ID=cds.MsaT025403.1;Parent=MsaT025403.1
Chr5 exon 7910743 7911053 7910743 ID=MsaT025403.1.exon4;Parent=MsaT025403.1
Chr5 CDS 7910743 7911053 7910743 ID=cds.MsaT025403.1;Parent=MsaT025403.1
Chr5 exon 7911141 7911261 7911141 ID=MsaT025403.1.exon5;Parent=MsaT025403.1
Chr5 CDS 7911141 7911261 7911141 ID=cds.MsaT025403.1;Parent=MsaT025403.1
Gene Sequence

>MsaT025403.1

ATGGCTAACATTCGGAATGCTAAGAAATTCATTCCGATACCACCTCCTTCGCCTCGATCAGCAGCAACCGGTTATCCTTGCGAGTATGCGGCGATGAATAATTTATCACCGAGAGGGCGAAGCAGCTGTGTCTCTTCTGCAACAAGAGATCTGTGGGAGCGTCTTTTCAATGAAGGGTATAAAGCCGATGTTTGCATTAATACTGATAACGGTGGCATTGTTTATGCTCATTCTAACATTATTGCGGTGGCTTCTCCTGTATTGAGAGGCATGCTGAAGCAAGCAAATCGTTCCAATCGGTGGCGATCGATCTCAATCTTTGGGGTTCCACATGATGCAGTACGAGTTTTTATTCGATATTTGTATTCTTCCTGTTACGAGAAAGAAGAAATGAAGGAATTTGTCCTACATTTGTTGGTGCTTTCTCATGTATACGCAGTCCCTCACTTGAAGCGTGAATGTGAACAGAAGCTAGAATTGGGTTTACTCACCGTGGATAATCTAGTTGATGTATTTCAACTTGCATTACTGTGTGATGCTCCGCGGCTCAGTCTCATTTGTCACCGTAAAATACTAAAGAACTTCAAAACTGTTTCGGAGTCAGAAGGATGGAAAGCAATGAAGCAGAGTCACCCAGTTCTAGAAAAGGAAATTCTGGAATCAATGATTGAAGAAGAAAATAGTAAGAAGGAGAGGATCAGAAAAATGAATGAAAGAGAGGTCTATTTGCAATTATACGATGCAATGGAAGCTCTAGTTCACATTTGTAGAGATGGTTGTCGAACTATTGGCCCGCATGATAAAGACTTCAAAGCAAACCAGCCGTGTAGGTATACTTCCTGCAAAGGGCTTGAATTGCTTGTTCGTCATTTTGCTGGTTGCAAGTTGAGAGTCCCTGGGGGTTGTGCTCATTGCAAGAGGATGTGGCAACTGTTGGAGCTGCATTCTCGACTGTGTGCTGATCCAGATTTCTGTAGAGTTCCTTTGTGCAGGAACTTTAAGCAGAGAATAGCAAAACAAAGCAAGAAAGACGAAATTAGATGGACAATTTTGGTTGAAAAGATCTTGAAAACAAGAGGAATTGGGATTGCACCATGCTTTCGGCCACAATGA

Protein sequence

>MsaT025403.1

MANIRNAKKFIPIPPPSPRSAATGYPCEYAAMNNLSPRGRSSCVSSATRDLWERLFNEGYKADVCINTDNGGIVYAHSNIIAVASPVLRGMLKQANRSNRWRSISIFGVPHDAVRVFIRYLYSSCYEKEEMKEFVLHLLVLSHVYAVPHLKRECEQKLELGLLTVDNLVDVFQLALLCDAPRLSLICHRKILKNFKTVSESEGWKAMKQSHPVLEKEILESMIEEENSKKERIRKMNEREVYLQLYDAMEALVHICRDGCRTIGPHDKDFKANQPCRYTSCKGLELLVRHFAGCKLRVPGGCAHCKRMWQLLELHSRLCADPDFCRVPLCRNFKQRIAKQSKKDEIRWTILVEKILKTRGIGIAPCFRPQ*