AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG025472

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025472 MtrunA17_Chr5g0401921 96.640 863 21 3 1 855 1 863 0.0 1707
MsaG025472 MtrunA17_Chr1g0194631 50.204 737 301 12 147 853 55 755 0.0 692
MsaG025472 MtrunA17_Chr7g0235081 58.166 349 134 9 512 854 3 345 8.59e-127 384
MsaG025472 MtrunA17_Chr7g0270001 62.782 266 94 3 592 853 32 296 6.93e-109 336
MsaG025472 MtrunA17_Chr7g0235091 36.041 197 104 3 145 341 53 227 2.78e-33 131
MsaG025472 MtrunA17_Chr7g0270011 40.496 121 67 1 218 338 66 181 9.26e-27 112
MsaG025472 MtrunA17_Chr1g0147121 33.974 156 96 3 687 835 873 1028 4.49e-21 99.4
MsaG025472 MtrunA17_Chr2g0297191 41.129 124 70 2 702 822 1027 1150 6.71e-21 99.0
MsaG025472 MtrunA17_Chr1g0201621 35.714 168 93 3 665 823 42 203 3.61e-20 94.7
MsaG025472 MtrunA17_Chr1g0201311 35.714 168 93 3 665 823 42 203 6.63e-20 94.0
MsaG025472 MtrunA17_Chr3g0126701 36.029 136 80 3 707 835 922 1057 6.74e-20 95.5
MsaG025472 MtrunA17_Chr8g0347071 32.353 136 90 2 692 825 793 928 1.21e-18 91.3
MsaG025472 MtrunA17_Chr7g0221611 39.669 121 70 2 705 822 904 1024 1.50e-18 91.3
MsaG025472 MtrunA17_Chr7g0275611 34.559 136 82 3 707 835 823 958 4.25e-18 89.7
MsaG025472 MtrunA17_Chr1g0149581 33.071 127 79 1 702 822 1517 1643 2.42e-16 84.3
MsaG025472 MtrunA17_Chr1g0149611 32.283 127 80 1 702 822 359 485 5.22e-16 82.0
MsaG025472 MtrunA17_Chr2g0305371 31.707 123 82 1 702 822 329 451 1.08e-15 80.9
MsaG025472 MtrunA17_Chr6g0477001 33.077 130 85 2 695 822 101 230 4.52e-14 74.7
MsaG025472 MtrunA17_Chr5g0403241 28.188 149 90 2 692 823 1338 1486 2.14e-12 71.2
MsaG025472 MtrunA17_Chr3g0125251 26.020 196 106 3 629 822 1284 1442 1.93e-11 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025472 AT2G23380.1 60.989 910 267 15 15 855 12 902 0.0 998
MsaG025472 AT2G23380.2 62.832 678 174 12 247 855 7 675 0.0 773
MsaG025472 AT4G02020.1 46.929 863 361 20 42 853 36 852 0.0 674
MsaG025472 AT4G02020.4 45.274 804 345 19 42 794 36 795 0.0 593
MsaG025472 AT4G02020.3 43.830 778 342 19 42 768 36 769 0.0 544
MsaG025472 AT4G02020.2 43.830 778 342 19 42 768 36 769 0.0 544
MsaG025472 AT1G02580.1 47.439 371 163 11 487 841 326 680 6.54e-103 334
MsaG025472 AT1G02580.1 29.682 283 163 11 42 307 17 280 5.59e-15 79.7
MsaG025472 AT1G02580.2 43.373 249 112 9 487 721 326 559 6.06e-55 200
MsaG025472 AT1G02580.2 29.682 283 163 11 42 307 17 280 4.43e-15 79.7
MsaG025472 AT5G42400.7 34.416 154 97 2 690 839 1233 1386 4.28e-21 100
MsaG025472 AT5G42400.2 34.416 154 97 2 690 839 1233 1386 4.28e-21 100
MsaG025472 AT5G42400.8 34.416 154 97 2 690 839 1233 1386 4.28e-21 100
MsaG025472 AT5G42400.5 34.416 154 97 2 690 839 1233 1386 4.28e-21 100
MsaG025472 AT5G42400.4 34.416 154 97 2 690 839 1233 1386 4.28e-21 100
MsaG025472 AT5G42400.1 34.416 154 97 2 690 839 1233 1386 4.28e-21 100
MsaG025472 AT5G42400.3 34.416 154 97 2 690 839 1233 1386 4.28e-21 100
MsaG025472 AT5G42400.6 34.416 154 97 2 690 839 1233 1386 4.28e-21 100
MsaG025472 AT2G31650.1 41.322 121 68 2 705 822 897 1017 9.46e-20 95.5
MsaG025472 AT4G27910.1 37.313 134 82 2 696 827 875 1008 9.58e-20 95.5
MsaG025472 AT5G53430.1 35.252 139 83 3 704 835 899 1037 9.60e-20 95.5
MsaG025472 AT1G76710.4 37.405 131 80 1 695 823 78 208 1.03e-18 90.9
MsaG025472 AT1G76710.2 37.405 131 80 1 695 823 78 208 1.10e-18 90.9
MsaG025472 AT1G76710.1 37.405 131 80 1 695 823 78 208 1.10e-18 90.9
MsaG025472 AT1G76710.3 37.405 131 80 1 695 823 78 208 1.26e-18 90.9
MsaG025472 AT3G61740.2 37.903 124 74 3 707 827 840 963 1.85e-18 91.3
MsaG025472 AT3G61740.1 35.766 137 80 4 707 835 876 1012 2.19e-18 91.3
MsaG025472 AT4G30860.1 32.317 164 93 5 663 822 295 444 3.12e-18 89.4
MsaG025472 AT1G05830.2 38.843 121 71 2 705 822 918 1038 3.74e-17 87.0
MsaG025472 AT1G05830.4 38.843 121 71 2 705 822 918 1038 3.74e-17 87.0
MsaG025472 AT1G05830.1 38.843 121 71 2 705 822 918 1038 3.74e-17 87.0
MsaG025472 AT1G05830.3 38.843 121 71 2 705 822 918 1038 3.74e-17 87.0
MsaG025472 AT3G59960.3 34.454 119 76 2 706 822 111 229 2.86e-15 77.8
MsaG025472 AT3G59960.2 34.454 119 76 2 706 822 111 229 2.86e-15 77.8
MsaG025472 AT3G59960.4 34.454 119 76 2 706 822 111 229 2.86e-15 77.8
MsaG025472 AT3G59960.1 34.454 119 76 2 706 822 111 229 4.39e-15 77.8
MsaG025472 AT1G77300.1 33.077 130 85 1 695 822 1015 1144 4.99e-13 73.9
MsaG025472 AT1G77300.2 33.077 130 85 1 695 822 1015 1144 6.07e-13 73.6
MsaG025472 AT2G44150.1 32.787 122 80 2 703 822 113 234 2.11e-12 70.1

Find 205 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAAAAGAATCATCAATT+TGG 0.149068 5:-8800925 None:intergenic
CTTAAAATGGCAGGTTATTA+TGG 0.213950 5:+8808575 MsaT025472.1:intron
AGCATGACTATCAGAGAATT+TGG 0.217139 5:+8800597 MsaT025472.1:intron
AATACCTTCGCGTAACAATT+TGG 0.232791 5:-8808474 None:intergenic
TTTACTTCAGGTAATTTAAT+AGG 0.247061 5:-8797405 None:intergenic
CTCACCGTTCGGAAACAATT+TGG 0.267793 5:-8801263 None:intergenic
CACGACATCGGCGACAAAAT+AGG 0.286588 5:-8800985 None:intergenic
GAGTGTCCTTGTCTTCTAAA+TGG 0.291259 5:+8805469 MsaT025472.1:CDS
ATATGCCTTCAGGTTCTTCT+TGG 0.300169 5:+8808031 MsaT025472.1:intron
CGAATTTATTATGATCAAAA+TGG 0.303568 5:+8799715 MsaT025472.1:CDS
TTAAGAGATTCCTGGCTATT+AGG 0.306752 5:-8805059 None:intergenic
GGTTCTACGGCAGTACTTCT+AGG 0.311438 5:+8797351 MsaT025472.1:CDS
CAGAAAAGAATCATCAATTT+GGG 0.315554 5:-8800924 None:intergenic
CTCTCAGGATCTTGTCTTCC+CGG 0.320449 5:+8801119 MsaT025472.1:CDS
CCTGGACGGGATGCTGCTTC+AGG 0.332402 5:+8801548 MsaT025472.1:CDS
AAGCATTTCAAAACAAGAAT+TGG 0.346654 5:+8801962 MsaT025472.1:CDS
GACGGTCTGATGTGTCTGGC+TGG 0.348610 5:+8808053 MsaT025472.1:CDS
TTGCTATAACTAACCAGATA+TGG 0.351637 5:+8797175 MsaT025472.1:CDS
TGGCAGGTTATTATGGTTGA+TGG 0.362435 5:+8808582 MsaT025472.1:intron
AGCTGTGGCGATGGCACTCT+TGG 0.363331 5:+8806693 MsaT025472.1:intron
CCGCCGACGATTTCCATATC+TGG 0.367263 5:-8797188 None:intergenic
TGGCTGGGGAGCCTTCTTAA+AGG 0.368220 5:+8808069 MsaT025472.1:CDS
ATGATAGGCAGCAGATTTCC+AGG 0.368503 5:-8805362 None:intergenic
ATAGCCAGGAATCTCTTAAA+TGG 0.370443 5:+8805063 MsaT025472.1:CDS
TGGTTTATCTGATGTTGTTT+TGG 0.372618 5:+8800617 MsaT025472.1:CDS
GGGAAGTTGTCTGGTTCTAC+TGG 0.374845 5:+8805132 MsaT025472.1:CDS
TTCCTCGCTTATCTGCTTCC+CGG 0.375191 5:-8808251 None:intergenic
CTTCTTCACAATTTATGTAC+TGG 0.376007 5:-8805110 None:intergenic
CCTCTGGCGGTGCTCTATCT+AGG 0.376930 5:+8801426 MsaT025472.1:CDS
TCTCTTTAATCTGAATGATC+AGG 0.377333 5:+8808302 MsaT025472.1:CDS
ATCGATTGGAGGGTCGGATC+TGG 0.383063 5:-8796008 None:intergenic
ACAGAGGATCAATCAGTATT+AGG 0.386098 5:+8799685 MsaT025472.1:CDS
GGGGAGAGCAATGGTTATAA+TGG 0.386785 5:+8797318 MsaT025472.1:CDS
TGACCGCTCACTGAATGATA+TGG 0.390718 5:+8801878 MsaT025472.1:CDS
TTCTTTGCACGACCACTTGA+AGG 0.396983 5:-8808747 None:intergenic
TCTTGGAAAACTCTTGAAAA+GGG 0.398591 5:+8801996 MsaT025472.1:CDS
TAGACCATTTAAGAGATTCC+TGG 0.401226 5:-8805067 None:intergenic
GAAACGAATTGTTACGGTTA+AGG 0.404174 5:+8797008 MsaT025472.1:CDS
CATGGTACATCTTCAGTGTT+AGG 0.412969 5:-8801236 None:intergenic
TGCAGTTAAAGACGTGTTAG+TGG 0.414048 5:+8796954 MsaT025472.1:CDS
CCAGTGGGGAAAAGTGGAAT+TGG 0.416857 5:+8801599 MsaT025472.1:CDS
TTATGGTTGATGGAGATCAT+AGG 0.420973 5:+8808592 MsaT025472.1:CDS
GAAGACGGAGAAGGCGGAGG+CGG 0.423270 5:-8795980 None:intergenic
AGCCCGATAGAGCTCCTGCT+TGG 0.425317 5:+8808679 MsaT025472.1:CDS
ATCTTGGAAAACTCTTGAAA+AGG 0.427773 5:+8801995 MsaT025472.1:CDS
AGCCCAAGCAGGAGCTCTAT+CGG 0.428962 5:-8808682 None:intergenic
GCTCTCCCCATCATCATTAC+CGG 0.429977 5:-8797304 None:intergenic
GAAGCAATTGACATGCATAA+TGG 0.434157 5:+8797273 MsaT025472.1:CDS
CTGCTGCCTATCATTCAATC+AGG 0.434225 5:+8805371 MsaT025472.1:CDS
AATTGGCTGATAATAAATCT+TGG 0.434669 5:+8801979 MsaT025472.1:CDS
ACTCCTTTCCACAAGCTGAT+TGG 0.436728 5:-8805450 None:intergenic
TTTGGTAAATACCTTTAAGA+AGG 0.436866 5:-8808080 None:intergenic
TTCTAGTTTGCATGCAGAAA+CGG 0.439068 5:+8801654 MsaT025472.1:CDS
TGTGAAGAAGGGAAGTTGTC+TGG 0.440207 5:+8805123 MsaT025472.1:CDS
GCCCGATAGAGCTCCTGCTT+GGG 0.440520 5:+8808680 MsaT025472.1:CDS
TTCTAGAAAAGCACTGAGCC+AGG 0.444807 5:-8800644 None:intergenic
GATTGCCTCCTTACACTACA+TGG 0.447081 5:+8797430 MsaT025472.1:CDS
TTCGCGTAACAATTTGGAAC+AGG 0.451100 5:-8808468 None:intergenic
GAAAAGGATTACAGAGAGAA+AGG 0.452779 5:+8805393 MsaT025472.1:CDS
TGCCGACTTATAGGTACTTA+AGG 0.453927 5:+8801352 MsaT025472.1:intron
ACGGTCTGATGTGTCTGGCT+GGG 0.459402 5:+8808054 MsaT025472.1:CDS
GCTGTGGCGATGGCACTCTT+GGG 0.460661 5:+8806694 MsaT025472.1:intron
CAGATGTTTGTAGGAACTGT+TGG 0.461588 5:+8805806 MsaT025472.1:CDS
CGGAGAGTTGATTTCTCACC+GGG 0.462898 5:+8808233 MsaT025472.1:CDS
CAACAGTTCCTACAAACATC+TGG 0.464408 5:-8805805 None:intergenic
CGGTCTGATGTGTCTGGCTG+GGG 0.465379 5:+8808055 MsaT025472.1:CDS
ACATGGATTGTACTGTCGGC+AGG 0.466976 5:-8805422 None:intergenic
GCCCAAGCAGGAGCTCTATC+GGG 0.467735 5:-8808681 None:intergenic
CTTGGACGGTCTGATGTGTC+TGG 0.470501 5:+8808049 MsaT025472.1:CDS
CAGATTACATGGATGCTCTC+AGG 0.470637 5:+8801104 MsaT025472.1:CDS
AATGTGATCCAGATGTTTGT+AGG 0.470951 5:+8805797 MsaT025472.1:CDS
GTCTGGATTTGCTGAGTAAG+AGG 0.471656 5:+8797244 MsaT025472.1:CDS
CCTATCATTCAATCAGGAAA+AGG 0.472188 5:+8805377 MsaT025472.1:CDS
ATTGCAGCTGCCTAATAGCC+AGG 0.472413 5:+8805049 MsaT025472.1:CDS
GCCTTCAGGTTCTTCTTGGA+CGG 0.474437 5:+8808035 MsaT025472.1:intron
CAGTACATAAATTGTGAAGA+AGG 0.479210 5:+8805111 MsaT025472.1:CDS
TAAATGGTCTAAAGACATGT+TGG 0.479310 5:+8805079 MsaT025472.1:CDS
AGTGACTCGGAGAATGGTCC+TGG 0.482458 5:+8801530 MsaT025472.1:CDS
GTTCTTGATGCTTATCGAAA+GGG 0.482842 5:+8808417 MsaT025472.1:CDS
GAAGAAGACGGAGAAGGCGG+AGG 0.483507 5:-8795983 None:intergenic
ACTAAACCAGTGGGGAAAAG+TGG 0.485809 5:+8801593 MsaT025472.1:CDS
CACGAGGAAAGAAGAATTTG+TGG 0.489043 5:+8801935 MsaT025472.1:CDS
GTCCATGCTTTGCTGCAGAT+AGG 0.489705 5:+8805773 MsaT025472.1:CDS
GTAATAATGAAGTGAGAAGA+AGG 0.492938 5:+8805293 MsaT025472.1:CDS
TCCATGCTTTGCTGCAGATA+GGG 0.497600 5:+8805774 MsaT025472.1:CDS
TAATTATCCCCTCTTTGACT+AGG 0.498579 5:-8806720 None:intergenic
AGGCTGAGAGGCAACCTTTG+TGG 0.498683 5:+8801207 MsaT025472.1:intron
AAATGGTCTAAAGACATGTT+GGG 0.502582 5:+8805080 MsaT025472.1:CDS
TTTGTAGGAACTGTTGGGTC+AGG 0.504264 5:+8805812 MsaT025472.1:CDS
GTTCGTGCCGGTAATGATGA+TGG 0.504381 5:+8797297 MsaT025472.1:CDS
TATGGTTGATGGAGATCATA+GGG 0.508403 5:+8808593 MsaT025472.1:CDS
CGGAAACAATTTGGCCCACA+TGG 0.509205 5:-8801254 None:intergenic
TCTTCTCAAACAGCAGCAAA+AGG 0.513002 5:+8806764 MsaT025472.1:CDS
TTCGTGCCGGTAATGATGAT+GGG 0.514702 5:+8797298 MsaT025472.1:CDS
TGCCGATACAGATAGAGGTC+TGG 0.518433 5:+8797227 MsaT025472.1:CDS
TAGATGAAGCACTTTCACTT+TGG 0.519134 5:-8801484 None:intergenic
AGATGTTTGTAGGAACTGTT+GGG 0.521591 5:+8805807 MsaT025472.1:CDS
GCTAAGGAACGGATTAATGC+TGG 0.522196 5:+8808627 MsaT025472.1:CDS
AAGTATACGGAAGGATCGAT+TGG 0.522345 5:-8796022 None:intergenic
GGAAGTTGTCTGGTTCTACT+GGG 0.522601 5:+8805133 MsaT025472.1:CDS
TCTGGCTTTCGAGCCCAAGC+AGG 0.523287 5:-8808693 None:intergenic
CCTGAAGCAGCATCCCGTCC+AGG 0.528406 5:-8801548 None:intergenic
CTCGGAGAATGGTCCTGGAC+GGG 0.531208 5:+8801535 MsaT025472.1:CDS
TGGAGATGACCATGGTTCTA+CGG 0.533083 5:+8797338 MsaT025472.1:CDS
GCTCGAAAGCCAGATGCCCC+TGG 0.535983 5:+8808702 MsaT025472.1:CDS
TGTTCTTGATGCTTATCGAA+AGG 0.537694 5:+8808416 MsaT025472.1:CDS
TGCTGCGAAACGAATTGTTA+CGG 0.538271 5:+8797002 MsaT025472.1:CDS
GATGTTGAAGACAAATCCTC+TGG 0.541597 5:+8801410 MsaT025472.1:CDS
GAGAGTAGTGACTCGGAGAA+TGG 0.542871 5:+8801524 MsaT025472.1:CDS
ATATCATTCAGTGAGCGGTC+AGG 0.544326 5:-8801876 None:intergenic
ATCCAGACCTCTATCTGTAT+CGG 0.545353 5:-8797229 None:intergenic
TCCCTATCTGCAGCAAAGCA+TGG 0.547017 5:-8805775 None:intergenic
ATCCACATGGATTGTACTGT+CGG 0.548401 5:-8805426 None:intergenic
AACAATTTGGAACAGGAGAG+TGG 0.550411 5:-8808461 None:intergenic
AGTCTGAGTAGATGTGACTT+TGG 0.553936 5:-8801387 None:intergenic
TACAATGCAGACAAAGAAAG+TGG 0.557922 5:+8796918 MsaT025472.1:intron
ATGCTGTAAAGAATGCTGTT+CGG 0.558439 5:+8797382 MsaT025472.1:CDS
CCGGAGAGTTGATTTCTCAC+CGG 0.558582 5:+8808232 MsaT025472.1:CDS
TTGGGATTCCTAGTCAAAGA+GGG 0.559307 5:+8806712 MsaT025472.1:CDS
CCGGTGAGAAATCAACTCTC+CGG 0.562064 5:-8808232 None:intergenic
TATTGTAGCAGCTGTGGCGA+TGG 0.563391 5:+8806684 MsaT025472.1:intron
AAAGTGCTTCATCTAATGCT+AGG 0.564163 5:+8801492 MsaT025472.1:CDS
CCCACAGTACTTCTCACAGC+AGG 0.565727 5:-8805494 None:intergenic
AGTACATAAATTGTGAAGAA+GGG 0.566712 5:+8805112 MsaT025472.1:CDS
TAGGAATATTTGCTAAGGAA+CGG 0.567191 5:+8808616 MsaT025472.1:CDS
GAATACCTTGGAGAGTATAC+CGG 0.569151 5:+8808213 MsaT025472.1:CDS
ACTAACACGTCTTTAACTGC+AGG 0.569514 5:-8796952 None:intergenic
CAGGTTCCATTTAGAAGACA+AGG 0.572098 5:-8805475 None:intergenic
TCCGTCGTTTAAAGTATACC+TGG 0.574401 5:+8805344 MsaT025472.1:CDS
GGAAGGATCGATTGGAGGGT+CGG 0.575587 5:-8796014 None:intergenic
TCTTCCCCACTCGTAACATG+TGG 0.577728 5:-8801909 None:intergenic
CATATCTGAGAGTAGTGACT+CGG 0.582166 5:+8801517 MsaT025472.1:CDS
GGAACTAATTGACGAAGAAG+AGG 0.585064 5:+8799771 MsaT025472.1:CDS
CATGGATTGTACTGTCGGCA+GGG 0.586600 5:-8805421 None:intergenic
TATACGGAAGGATCGATTGG+AGG 0.587323 5:-8796019 None:intergenic
AGGCGGAGAGTTTGACGCCA+TGG 0.588142 5:-8795963 None:intergenic
CTGGAAGAAGAAGACGGAGA+AGG 0.588519 5:-8795989 None:intergenic
TCCAGGTATACTTTAAACGA+CGG 0.589735 5:-8805345 None:intergenic
CAAGCTGATTGGCATCCACA+TGG 0.591796 5:-8805439 None:intergenic
ATTTATTATGATCAAAATGG+TGG 0.591896 5:+8799718 MsaT025472.1:CDS
GCTACAAATTCTCTCGTGGA+AGG 0.592737 5:+8805159 MsaT025472.1:CDS
CCACCTAAAACTAAACCAGT+GGG 0.597750 5:+8801584 MsaT025472.1:CDS
TGTTCCAAATTGTTACGCGA+AGG 0.599246 5:+8808470 MsaT025472.1:CDS
ACTCTCCGGTATACTCTCCA+AGG 0.599359 5:-8808218 None:intergenic
TCTGGAAAAGGATCTTGAAG+CGG 0.601715 5:+8800943 MsaT025472.1:CDS
CTGACCCACATGTTACGAGT+GGG 0.602989 5:+8801904 MsaT025472.1:CDS
TAGGGTAGGAATATTTGCTA+AGG 0.603131 5:+8808611 MsaT025472.1:CDS
GTTTGTAAACATGAATACCT+TGG 0.604817 5:+8808201 MsaT025472.1:CDS
AGCATGGACATTGACGACTT+CGG 0.607089 5:-8805759 None:intergenic
ATGCATAATGGTGTTCGTGC+CGG 0.607974 5:+8797285 MsaT025472.1:CDS
CCTAGATAGAGCACCGCCAG+AGG 0.611003 5:-8801426 None:intergenic
TCGTCGGCGGAGAGGACGTG+TGG 0.611446 5:+8797201 MsaT025472.1:CDS
ACTCGGAGAATGGTCCTGGA+CGG 0.611892 5:+8801534 MsaT025472.1:CDS
GGATGCTACAAATTCTCTCG+TGG 0.612099 5:+8805155 MsaT025472.1:CDS
GTTGATGGAGATCATAGGGT+AGG 0.614383 5:+8808597 MsaT025472.1:CDS
CTTGGGATTCCTAGTCAAAG+AGG 0.614392 5:+8806711 MsaT025472.1:CDS
TATGGAAATCGTCGGCGGAG+AGG 0.615136 5:+8797193 MsaT025472.1:CDS
AATGATGATGGGGAGAGCAA+TGG 0.615333 5:+8797309 MsaT025472.1:CDS
GGCATTGCCGATACAGATAG+AGG 0.618454 5:+8797222 MsaT025472.1:CDS
GAGCTCTATCGGGCTCGTAA+CGG 0.619502 5:-8808671 None:intergenic
AGGATCAATCAGTATTAGGT+CGG 0.619655 5:+8799689 MsaT025472.1:CDS
GAAGAAGAAGACGGAGAAGG+CGG 0.621138 5:-8795986 None:intergenic
TGTGGATGCCAATCAGCTTG+TGG 0.621426 5:+8805442 MsaT025472.1:CDS
TCTCGTGGAAGGCTATTCCA+AGG 0.624684 5:+8805170 MsaT025472.1:CDS
GGAACGGATTAATGCTGGCG+AGG 0.624928 5:+8808632 MsaT025472.1:CDS
ATCAATCAGTATTAGGTCGG+AGG 0.625781 5:+8799692 MsaT025472.1:CDS
GAAGACGGTGCTCCTTCAAG+TGG 0.626003 5:+8808735 MsaT025472.1:CDS
CAATGAAGACAATAGTGGCG+AGG 0.626663 5:+8800871 MsaT025472.1:CDS
ATGCCAATCAGCTTGTGGAA+AGG 0.628895 5:+8805447 MsaT025472.1:CDS
ATACGGAAGGATCGATTGGA+GGG 0.630832 5:-8796018 None:intergenic
TTTGGAATAACAGAATAGAG+TGG 0.636263 5:+8797131 MsaT025472.1:intron
TGGGCCAAATTGTTTCCGAA+CGG 0.639399 5:+8801259 MsaT025472.1:CDS
AAAATCCATGTAGTGTAAGG+AGG 0.639629 5:-8797435 None:intergenic
GAAAGAATCTTACACGACAT+CGG 0.641819 5:-8800997 None:intergenic
TCTGACCCACATGTTACGAG+TGG 0.642977 5:+8801903 MsaT025472.1:CDS
CCCACCTAAAACTAAACCAG+TGG 0.643501 5:+8801583 MsaT025472.1:CDS
TCGGATCTGGAAGAAGAAGA+CGG 0.647493 5:-8795995 None:intergenic
CAGAAATCAAAGAATGACAG+AGG 0.651131 5:+8799669 MsaT025472.1:intron
GTGTTAGGAGGAGTCCACAA+AGG 0.652487 5:-8801221 None:intergenic
TATTTGCAGCGACAGTGAGG+AGG 0.654413 5:+8799750 MsaT025472.1:CDS
CTTCCCCACTCGTAACATGT+GGG 0.655437 5:-8801908 None:intergenic
GGAATAACAGAATAGAGTGG+AGG 0.658661 5:+8797134 MsaT025472.1:intron
AAACCATATCATTCAGTGAG+CGG 0.659543 5:-8801881 None:intergenic
GAAGTACTGCCGTAGAACCA+TGG 0.660327 5:-8797347 None:intergenic
GGTACATCTTCAGTGTTAGG+AGG 0.660539 5:-8801233 None:intergenic
TAACCAGATATGGAAATCGT+CGG 0.662231 5:+8797185 MsaT025472.1:CDS
TAAGAGAAACAGTAAGCGAG+TGG 0.662571 5:+8801622 MsaT025472.1:CDS
TGACCCACATGTTACGAGTG+GGG 0.665377 5:+8801905 MsaT025472.1:CDS
ACCTGCTGTGAGAAGTACTG+TGG 0.669440 5:+8805493 MsaT025472.1:CDS
CCTGCTGTGAGAAGTACTGT+GGG 0.671307 5:+8805494 MsaT025472.1:CDS
GGTTATAATGGAGATGACCA+TGG 0.672080 5:+8797330 MsaT025472.1:CDS
AATGCAGACAAAGAAAGTGG+AGG 0.674743 5:+8796921 MsaT025472.1:intron
CGAGTGGGGAAGACAACACG+AGG 0.677101 5:+8801919 MsaT025472.1:CDS
ACTTATTTGCAGCGACAGTG+AGG 0.677851 5:+8799747 MsaT025472.1:CDS
AACACTGAAGATGTACCATG+TGG 0.679817 5:+8801239 MsaT025472.1:CDS
TGGGATTCCTAGTCAAAGAG+GGG 0.679854 5:+8806713 MsaT025472.1:CDS
TGCCGACAGTACAATCCATG+TGG 0.680454 5:+8805424 MsaT025472.1:CDS
TCGTGCCGGTAATGATGATG+GGG 0.694352 5:+8797299 MsaT025472.1:CDS
TGGTTCATGAATAGGCTGAG+AGG 0.694664 5:+8801195 MsaT025472.1:intron
CACCGGGAAGCAGATAAGCG+AGG 0.695786 5:+8808249 MsaT025472.1:CDS
CCAGATATGGAAATCGTCGG+CGG 0.697154 5:+8797188 MsaT025472.1:CDS
GAAGTTGTCTGGTTCTACTG+GGG 0.701883 5:+8805134 MsaT025472.1:CDS
ACACTGAAGATGTACCATGT+GGG 0.703901 5:+8801240 MsaT025472.1:CDS
GTTGAAGACAAATCCTCTGG+CGG 0.737906 5:+8801413 MsaT025472.1:CDS
CACCTAAAACTAAACCAGTG+GGG 0.751699 5:+8801585 MsaT025472.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 8795965 8808779 8795965 ID=MsaG025472
Chr5 mRNA 8795965 8808779 8795965 ID=MsaT025472.1;Parent=MsaG025472
Chr5 exon 8795965 8796036 8795965 ID=MsaT025472.1.exon1;Parent=MsaT025472.1
Chr5 CDS 8795965 8796036 8795965 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8796928 8797029 8796928 ID=MsaT025472.1.exon2;Parent=MsaT025472.1
Chr5 CDS 8796928 8797029 8796928 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8797144 8797466 8797144 ID=MsaT025472.1.exon3;Parent=MsaT025472.1
Chr5 CDS 8797144 8797466 8797144 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8799672 8799833 8799672 ID=MsaT025472.1.exon4;Parent=MsaT025472.1
Chr5 CDS 8799672 8799833 8799672 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8800599 8800683 8800599 ID=MsaT025472.1.exon5;Parent=MsaT025472.1
Chr5 CDS 8800599 8800683 8800599 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8800851 8801006 8800851 ID=MsaT025472.1.exon6;Parent=MsaT025472.1
Chr5 CDS 8800851 8801006 8800851 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8801093 8801140 8801093 ID=MsaT025472.1.exon7;Parent=MsaT025472.1
Chr5 CDS 8801093 8801140 8801093 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8801209 8801280 8801209 ID=MsaT025472.1.exon8;Parent=MsaT025472.1
Chr5 CDS 8801209 8801280 8801209 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8801365 8801712 8801365 ID=MsaT025472.1.exon9;Parent=MsaT025472.1
Chr5 CDS 8801365 8801712 8801365 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8801867 8802017 8801867 ID=MsaT025472.1.exon10;Parent=MsaT025472.1
Chr5 CDS 8801867 8802017 8801867 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8805037 8805191 8805037 ID=MsaT025472.1.exon11;Parent=MsaT025472.1
Chr5 CDS 8805037 8805191 8805037 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8805292 8805515 8805292 ID=MsaT025472.1.exon12;Parent=MsaT025472.1
Chr5 CDS 8805292 8805515 8805292 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8805702 8805833 8805702 ID=MsaT025472.1.exon13;Parent=MsaT025472.1
Chr5 CDS 8805702 8805833 8805702 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8806695 8806785 8806695 ID=MsaT025472.1.exon14;Parent=MsaT025472.1
Chr5 CDS 8806695 8806785 8806695 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8808043 8808090 8808043 ID=MsaT025472.1.exon15;Parent=MsaT025472.1
Chr5 CDS 8808043 8808090 8808043 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8808195 8808323 8808195 ID=MsaT025472.1.exon16;Parent=MsaT025472.1
Chr5 CDS 8808195 8808323 8808195 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8808414 8808491 8808414 ID=MsaT025472.1.exon17;Parent=MsaT025472.1
Chr5 CDS 8808414 8808491 8808414 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Chr5 exon 8808588 8808779 8808588 ID=MsaT025472.1.exon18;Parent=MsaT025472.1
Chr5 CDS 8808588 8808779 8808588 ID=cds.MsaT025472.1;Parent=MsaT025472.1
Gene Sequence

>MsaT025472.1

ATGGCGTCAAACTCTCCGCCTCCGCCTTCTCCGTCTTCTTCTTCCAGATCCGACCCTCCAATCGATCCTTCCACAAAGAAAGTGGAGGAAACAAGTCCTGCAGTTAAAGACGTGTTAGTGGTTATTGAATCTTTGAAGAAGCAAGTTGCTGCGAAACGAATTGTTACGGTTAAGAATAGAGTGGAGGAAAATAGACAGAAGCTGATTGCTATAACTAACCAGATATGGAAATCGTCGGCGGAGAGGACGTGTGGCATTGCCGATACAGATAGAGGTCTGGATTTGCTGAGTAAGAGGCAAAAAGAAGCAATTGACATGCATAATGGTGTTCGTGCCGGTAATGATGATGGGGAGAGCAATGGTTATAATGGAGATGACCATGGTTCTACGGCAGTACTTCTAGGATCTAATTATGCTGTAAAGAATGCTGTTCGGCCTATTAAATTACCTGAAGTAAAAAGATTGCCTCCTTACACTACATGGATTTTTCTGGACAGAAATCAAAGAATGACAGAGGATCAATCAGTATTAGGTCGGAGGCGAATTTATTATGATCAAAATGGTGGTGAAGCACTTATTTGCAGCGACAGTGAGGAGGAACTAATTGACGAAGAAGAGGAAAAAAGAGAATTTGTAGAATCAGAAGATTTTATACTTCGCATGACTATCAGAGAATTTGGTTTATCTGATGTTGTTTTGGAGATCCTGGCTCAGTGCTTTTCTAGAAAAACTAGTGACATTAAGGCCAGATATGAAACTTTTTGCAATGAAGACAATAGTGGCGAGGATTCTAAAAATGGAGATGCACAAGATAATTCCCAAATTGATGATTCTTTTCTGGAAAAGGATCTTGAAGCGGCTCTTGACTCTTTTGACAACCTATTTTGTCGCCGATGTCGTGTTTTCGATTGCAGATTACATGGATGCTCTCAGGATCTTGTCTTCCCGGCTGAGAGGCAACCTTTGTGGACTCCTCCTAACACTGAAGATGTACCATGTGGGCCAAATTGTTTCCGAACGGTACTTAAGGCAGAAAAAATGGCCAAAGTCACATCTACTCAGACTGATGTTGAAGACAAATCCTCTGGCGGTGCTCTATCTAGGAAAAAGTCTTCCGGTAGAAGGCGTATAAAATGCAGCCAAAGTGAAAGTGCTTCATCTAATGCTAGGATCATATCTGAGAGTAGTGACTCGGAGAATGGTCCTGGACGGGATGCTGCTTCAGGCTCCCACTCAGCCCCACCTAAAACTAAACCAGTGGGGAAAAGTGGAATTGGTAAGAGAAACAGTAAGCGAGTGGCAGAACGCGTTCTAGTTTGCATGCAGAAACGGCAGAAGAAAACAGTAGCTTCTGATTCTGATTCCATTAGTGAAGCTCCTGACCGCTCACTGAATGATATGGTTTCTGACCCACATGTTACGAGTGGGGAAGACAACACGAGGAAAGAAGAATTTGTGGATGAAAGCATTTCAAAACAAGAATTGGCTGATAATAAATCTTGGAAAACTCTTGAAAAGGTAACTGATTTTTATTGCAGCTGCCTAATAGCCAGGAATCTCTTAAATGGTCTAAAGACATGTTGGGATGTTTTCCAGTACATAAATTGTGAAGAAGGGAAGTTGTCTGGTTCTACTGGGGATGCTACAAATTCTCTCGTGGAAGGCTATTCCAAGGGTAATAATGAAGTGAGAAGAAGGTCAAAATTTTTACGTAGAAGAGGAAGAGTCCGTCGTTTAAAGTATACCTGGAAATCTGCTGCCTATCATTCAATCAGGAAAAGGATTACAGAGAGAAAGGATCAGCCCTGCCGACAGTACAATCCATGTGGATGCCAATCAGCTTGTGGAAAGGAGTGTCCTTGTCTTCTAAATGGAACCTGCTGTGAGAAGTACTGTGGATGCCCCAAGAGTTGTAAAAATCGATTTCGAGGCTGTCATTGTGCGAAGAGTCAATGCCGAAGTCGTCAATGTCCATGCTTTGCTGCAGATAGGGAATGTGATCCAGATGTTTGTAGGAACTGTTGGGTCAGCTGTGGCGATGGCACTCTTGGGATTCCTAGTCAAAGAGGGGATAATTATGAATGTAGAAACATGAAGCTTCTTCTCAAACAGCAGCAAAAGGTTCTTCTTGGACGGTCTGATGTGTCTGGCTGGGGAGCCTTCTTAAAGAATAGCGTTTGTAAACATGAATACCTTGGAGAGTATACCGGAGAGTTGATTTCTCACCGGGAAGCAGATAAGCGAGGAAAGATATATGACCGTGAAAATTCATCTTTTCTCTTTAATCTGAATGATCAGTTTGTTCTTGATGCTTATCGAAAGGGTGATAAATTGAAGTTTGCAAACCACTCTCCTGTTCCAAATTGTTACGCGAAGGTTATTATGGTTGATGGAGATCATAGGGTAGGAATATTTGCTAAGGAACGGATTAATGCTGGCGAGGAGCTGTTTTATGATTACCGTTACGAGCCCGATAGAGCTCCTGCTTGGGCTCGAAAGCCAGATGCCCCTGGATCGAAAAAAGAAGACGGTGCTCCTTCAAGTGGTCGTGCAAAGAAGCTTGCTTAA
Protein sequence

>MsaT025472.1

MASNSPPPPSPSSSSRSDPPIDPSTKKVEETSPAVKDVLVVIESLKKQVAAKRIVTVKNRVEENRQKLIAITNQIWKSSAERTCGIADTDRGLDLLSKRQKEAIDMHNGVRAGNDDGESNGYNGDDHGSTAVLLGSNYAVKNAVRPIKLPEVKRLPPYTTWIFLDRNQRMTEDQSVLGRRRIYYDQNGGEALICSDSEEELIDEEEEKREFVESEDFILRMTIREFGLSDVVLEILAQCFSRKTSDIKARYETFCNEDNSGEDSKNGDAQDNSQIDDSFLEKDLEAALDSFDNLFCRRCRVFDCRLHGCSQDLVFPAERQPLWTPPNTEDVPCGPNCFRTVLKAEKMAKVTSTQTDVEDKSSGGALSRKKSSGRRRIKCSQSESASSNARIISESSDSENGPGRDAASGSHSAPPKTKPVGKSGIGKRNSKRVAERVLVCMQKRQKKTVASDSDSISEAPDRSLNDMVSDPHVTSGEDNTRKEEFVDESISKQELADNKSWKTLEKVTDFYCSCLIARNLLNGLKTCWDVFQYINCEEGKLSGSTGDATNSLVEGYSKGNNEVRRRSKFLRRRGRVRRLKYTWKSAAYHSIRKRITERKDQPCRQYNPCGCQSACGKECPCLLNGTCCEKYCGCPKSCKNRFRGCHCAKSQCRSRQCPCFAADRECDPDVCRNCWVSCGDGTLGIPSQRGDNYECRNMKLLLKQQQKVLLGRSDVSGWGAFLKNSVCKHEYLGEYTGELISHREADKRGKIYDRENSSFLFNLNDQFVLDAYRKGDKLKFANHSPVPNCYAKVIMVDGDHRVGIFAKERINAGEELFYDYRYEPDRAPAWARKPDAPGSKKEDGAPSSGRAKKLA*