AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG025500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025500 MtrunA17_Chr5g0402161 91.633 251 15 2 1 249 1 247 4.00e-140 393
MsaG025500 MtrunA17_Chr8g0375121 57.519 266 80 10 1 249 1 250 1.58e-65 204
MsaG025500 MtrunA17_Chr3g0134001 54.545 209 76 7 25 214 40 248 1.58e-58 186
MsaG025500 MtrunA17_Chr8g0384141 75.824 91 22 0 112 202 87 177 7.74e-47 156
MsaG025500 MtrunA17_Chr2g0330641 56.923 130 48 3 83 205 228 356 2.66e-31 119
MsaG025500 MtrunA17_Chr8g0341421 63.636 99 32 1 111 205 296 394 3.26e-29 114
MsaG025500 MtrunA17_Chr7g0243041 68.966 58 18 0 145 202 32 89 3.52e-23 92.0
MsaG025500 MtrunA17_Chr5g0435021 55.696 79 33 1 125 201 202 280 1.74e-20 88.6
MsaG025500 MtrunA17_Chr1g0207301 53.731 67 31 0 135 201 284 350 1.65e-17 80.9
MsaG025500 MtrunA17_Chr1g0152651 62.963 54 20 0 148 201 283 336 3.06e-17 80.1
MsaG025500 MtrunA17_Chr5g0393721 62.963 54 20 0 148 201 233 286 4.62e-17 79.3
MsaG025500 MtrunA17_Chr1g0207311 51.948 77 32 2 130 201 182 258 7.28e-17 78.2
MsaG025500 MtrunA17_Chr3g0134741 55.556 63 28 0 139 201 229 291 2.64e-16 77.0
MsaG025500 MtrunA17_Chr4g0074161 54.286 70 27 1 146 210 213 282 8.19e-15 73.2
MsaG025500 MtrunA17_Chr1g0190731 51.429 70 34 0 132 201 186 255 8.60e-15 72.8
MsaG025500 MtrunA17_Chr1g0179061 42.169 83 42 1 139 215 170 252 3.32e-13 67.4
MsaG025500 MtrunA17_Chr2g0311101 46.667 75 33 1 135 202 158 232 7.65e-13 67.0
MsaG025500 MtrunA17_Chr4g0014771 50.769 65 31 1 140 203 119 183 4.40e-12 63.2
MsaG025500 MtrunA17_Chr3g0095391 39.437 71 43 0 150 220 320 390 6.66e-11 61.6
MsaG025500 MtrunA17_Chr2g0323091 48.148 54 28 0 149 202 146 199 6.66e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025500 AT3G50330.1 55.046 218 67 7 14 222 4 199 7.26e-51 166
MsaG025500 AT5G67060.1 57.292 192 68 4 17 203 4 186 1.30e-50 166
MsaG025500 AT5G67060.2 56.831 183 66 3 25 203 1 174 1.71e-47 157
MsaG025500 AT4G00120.1 67.708 96 28 1 114 206 87 182 1.21e-39 136
MsaG025500 AT3G21330.1 65.957 94 32 0 111 204 241 334 6.17e-38 136
MsaG025500 AT5G09750.1 76.667 90 21 0 112 201 92 181 2.31e-35 126
MsaG025500 AT5G01305.1 69.492 59 18 0 140 198 38 96 7.97e-22 89.4
MsaG025500 AT5G43175.1 50.725 69 34 0 133 201 126 194 8.25e-19 82.4
MsaG025500 AT4G33880.1 59.677 62 25 0 140 201 267 328 1.65e-18 84.0
MsaG025500 AT5G37800.1 56.164 73 25 1 136 201 191 263 4.20e-18 82.0
MsaG025500 AT1G27740.1 51.471 68 33 0 134 201 162 229 5.91e-18 80.9
MsaG025500 AT1G66470.1 67.273 55 18 0 147 201 203 257 6.43e-18 81.6
MsaG025500 AT2G14760.1 58.065 62 26 0 140 201 238 299 1.79e-17 80.5
MsaG025500 AT4G30980.1 43.434 99 55 1 103 201 95 192 1.01e-16 78.2
MsaG025500 AT2G24260.2 52.703 74 29 1 134 201 24 97 1.43e-16 77.0
MsaG025500 AT4G30980.2 43.434 99 55 1 103 201 143 240 2.52e-16 77.8
MsaG025500 AT2G24260.1 52.703 74 29 1 134 201 127 200 3.64e-16 77.0
MsaG025500 AT5G58010.1 54.545 66 30 0 136 201 96 161 9.32e-15 72.8
MsaG025500 AT2G14760.3 48.000 75 26 1 140 201 238 312 3.91e-14 71.2

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGTGGTTTAATGGTTTC+TGG 0.032783 5:-9012122 None:intergenic
CTTTCTCTTTGGTGGTTTAA+TGG 0.129321 5:-9012129 None:intergenic
ACAAACAATGATTTCCCTTA+TGG 0.235794 5:+9011873 MsaT025500.1:CDS
ATCTTCACATTCTTTCTCTT+TGG 0.246196 5:-9012140 None:intergenic
AATTATTCTTCTTTGCTTAG+AGG 0.274535 5:+9012401 MsaT025500.1:CDS
AAAGAATTAGTGAAAGAATA+AGG 0.285456 5:+9012204 MsaT025500.1:CDS
GAGATTGAGAGTGTTGTTAT+TGG 0.293893 5:-9011844 None:intergenic
TTATTGGTATTGCTATAAAA+AGG 0.306430 5:-9011828 None:intergenic
TTGGTGGTTTAATGGTTTCT+GGG 0.314323 5:-9012121 None:intergenic
TGTCTTGCTGCTACACTTTG+TGG 0.337683 5:-9012173 None:intergenic
TTCACATTCTTTCTCTTTGG+TGG 0.352271 5:-9012137 None:intergenic
TGAAGCGACGGCGGTGTCAT+TGG 0.364263 5:-9011966 None:intergenic
TTCAAACGTTGGAACAAGTT+GGG 0.370508 5:+9012321 MsaT025500.1:CDS
TTGAATGTTGTTGGGTTTCA+AGG 0.375435 5:+9012356 MsaT025500.1:CDS
GTTTCTGGGTCTATGTTAAT+CGG 0.384832 5:-9012107 None:intergenic
TGATGGGATTTGAACTCTGT+TGG 0.387374 5:-9011991 None:intergenic
GCCATTGAATTCTTCTTCTC+CGG 0.399282 5:-9012044 None:intergenic
ACTAAGCATTTGTTTGGAAG+TGG 0.402015 5:-9012453 None:intergenic
AATAGACCTTTGAATGTTGT+TGG 0.403761 5:+9012347 MsaT025500.1:CDS
CATTTCTTCATTCTCCTATC+CGG 0.405852 5:+9012025 MsaT025500.1:CDS
GTTACATTTGAGTTTCCATA+AGG 0.409196 5:-9011888 None:intergenic
TGAGGACATACTGAAAACTT+TGG 0.409321 5:+9011734 MsaT025500.1:CDS
TGGAAGTGGGACCCACCATT+TGG 0.414540 5:-9012439 None:intergenic
GATGGGATTTGAACTCTGTT+GGG 0.418955 5:-9011990 None:intergenic
GTGTCAACTAAGCATTTGTT+TGG 0.421668 5:-9012459 None:intergenic
TTCATCACCTTGCCAAATGG+TGG 0.447650 5:+9012427 MsaT025500.1:CDS
GAGGACATACTGAAAACTTT+GGG 0.447841 5:+9011735 MsaT025500.1:CDS
ATAGACCTTTGAATGTTGTT+GGG 0.458742 5:+9012348 MsaT025500.1:CDS
TTACATTTGAGTTTCCATAA+GGG 0.484501 5:-9011887 None:intergenic
GTTCAAACGTTGGAACAAGT+TGG 0.486495 5:+9012320 MsaT025500.1:CDS
GATGACAATGATGATGCAAA+TGG 0.498844 5:+9011782 MsaT025500.1:CDS
TGAATTCTTCTTCTCCGGAT+AGG 0.501360 5:-9012039 None:intergenic
AAATGTTGGTTCTTCCTGAT+GGG 0.503530 5:-9012007 None:intergenic
TTGTTCATCACCTTGCCAAA+TGG 0.505893 5:+9012424 MsaT025500.1:CDS
AACAGAGTTCAAATCCCATC+AGG 0.513806 5:+9011993 MsaT025500.1:CDS
TGTTGTTGGGTTTCAAGGAG+TGG 0.515205 5:+9012361 MsaT025500.1:CDS
GAAATGTTGGTTCTTCCTGA+TGG 0.537381 5:-9012008 None:intergenic
TCTGAAAACATTCATGGATG+AGG 0.543834 5:+9011716 None:intergenic
CTAAGCATTTGTTTGGAAGT+GGG 0.548242 5:-9012452 None:intergenic
GGTGTCATTGGTTGTTGAAG+AGG 0.548657 5:-9011954 None:intergenic
ATAGGAGAATGAAGAAATGT+TGG 0.554824 5:-9012021 None:intergenic
GAAGAAACAAGTTCAAACGT+TGG 0.554879 5:+9012310 MsaT025500.1:CDS
TCATCACCTTGCCAAATGGT+GGG 0.555921 5:+9012428 MsaT025500.1:CDS
TGAAACCCAACAACATTCAA+AGG 0.560330 5:-9012353 None:intergenic
TCAAACGTTGGAACAAGTTG+GGG 0.569007 5:+9012322 MsaT025500.1:CDS
ACTCTGTTGGGTTGAAGCGA+CGG 0.575367 5:-9011978 None:intergenic
AAAGAATGTGAAGATTTCAA+AGG 0.590303 5:+9012148 MsaT025500.1:CDS
TGAAGAGGATAAGACAATGG+TGG 0.592428 5:-9011939 None:intergenic
TCCGGAGAAGAAGAATTCAA+TGG 0.605617 5:+9012043 MsaT025500.1:CDS
AGAGATGATATTTCGTATAG+CGG 0.621555 5:+9012076 MsaT025500.1:CDS
AATGGTGGATTGATCAATGA+AGG 0.629442 5:-9011924 None:intergenic
GGGAAATGTTGAAAGTAACA+TGG 0.631569 5:+9011755 MsaT025500.1:CDS
GTGGGACCCACCATTTGGCA+AGG 0.634945 5:-9012434 None:intergenic
CTGTTGGGTTGAAGCGACGG+CGG 0.671897 5:-9011975 None:intergenic
ATATTACAAAGATTAGTACC+TGG 0.683168 5:+9012227 MsaT025500.1:CDS
TTACAAAGATTAGTACCTGG+TGG 0.689802 5:+9012230 MsaT025500.1:CDS
TGTTGAAGAGGATAAGACAA+TGG 0.700529 5:-9011942 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 9011729 9012478 9011729 ID=MsaG025500
Chr5 mRNA 9011729 9012478 9011729 ID=MsaT025500.1;Parent=MsaG025500
Chr5 exon 9011729 9012478 9011729 ID=MsaT025500.1.exon1;Parent=MsaT025500.1
Chr5 CDS 9011729 9012478 9011729 ID=cds.MsaT025500.1;Parent=MsaT025500.1
Gene Sequence

>MsaT025500.1

ATGGATGAGGACATACTGAAAACTTTGGGAAATGTTGAAAGTAACATGGACATGATGACAATGATGATGCAAATGGAAAAGTTCCCTGATTCATATGAACCTTTTTATAGCAATACCAATAACAACACTCTCAATCTCAATCTCACAAACAATGATTTCCCTTATGGAAACTCAAATGTAACTTCATCACTCACACCTTCATTGATCAATCCACCATTGTCTTATCCTCTTCAACAACCAATGACACCGCCGTCGCTTCAACCCAACAGAGTTCAAATCCCATCAGGAAGAACCAACATTTCTTCATTCTCCTATCCGGAGAAGAAGAATTCAATGGCAGCAATGAGAGAGATGATATTTCGTATAGCGGTTATGCAACCGATTAACATAGACCCAGAAACCATTAAACCACCAAAGAGAAAGAATGTGAAGATTTCAAAGGATCCACAAAGTGTAGCAGCAAGACATAGAAGAGAAAGAATTAGTGAAAGAATAAGGATATTACAAAGATTAGTACCTGGTGGAACAAAAATGGACACTGCTTCTATGTTAGATGAAGCAATACATTATGTGAAGTTTTTGAAGAAACAAGTTCAAACGTTGGAACAAGTTGGGGCAAATAGACCTTTGAATGTTGTTGGGTTTCAAGGAGTGGTTTCTAATGCTAATTTTAATTATTCTTCTTTGCTTAGAGGTTGTTCATCACCTTGCCAAATGGTGGGTCCCACTTCCAAACAAATGCTTAGTTGA

Protein sequence

>MsaT025500.1

MDEDILKTLGNVESNMDMMTMMMQMEKFPDSYEPFYSNTNNNTLNLNLTNNDFPYGNSNVTSSLTPSLINPPLSYPLQQPMTPPSLQPNRVQIPSGRTNISSFSYPEKKNSMAAMREMIFRIAVMQPINIDPETIKPPKRKNVKISKDPQSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIHYVKFLKKQVQTLEQVGANRPLNVVGFQGVVSNANFNYSSLLRGCSSPCQMVGPTSKQMLS*