AlfalfaGEDB | MsaG025542

M. sativa ssp. caerulea / MsaG025542

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025542	RHN54067.1	95.062	324	16	0	2	325	24	347	0.0	571

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025542	sp\|F4JRB0\|HHO5_ARATH	39.295	369	156	12	6	329	12	357	1.43e-56	190

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025542	tr\|A0A396HTM4\|A0A396HTM4_MEDTR	95.062	324	16	0	2	325	24	347	0.0	571

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TFs/TRs:

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Gene ID	Type	Classification
MsaG025542	TF	GARP-G2-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000015	MsaG025542	0.802584	8.794260e-49	1.260844e-46
MsaG000030	MsaG025542	0.823140	2.870526e-53	6.864518e-51
MsaG000045	MsaG025542	0.872105	8.409106e-67	1.065889e-63
MsaG000052	MsaG025542	0.818472	3.359319e-52	7.097601e-50
MsaG000070	MsaG025542	0.829816	7.481889e-55	2.153884e-52
MsaG000083	MsaG025542	0.886712	5.842790e-72	1.456289e-68
MsaG000113	MsaG025542	0.879195	3.196046e-69	5.558851e-66
MsaG000151	MsaG025542	0.864714	1.989844e-64	1.858576e-61
MsaG000153	MsaG025542	0.852325	9.527955e-61	5.598412e-58
MsaG000188	MsaG025542	0.837688	8.231719e-57	2.990158e-54

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025542	MtrunA17_Chr5g0402631	95.062	324	16	0	2	325	24	347	0.0	571
MsaG025542	MtrunA17_Chr4g0045361	39.268	410	149	13	5	324	8	407	1.49e-65	212
MsaG025542	MtrunA17_Chr4g0062921	35.505	307	139	9	9	263	6	305	2.58e-40	146
MsaG025542	MtrunA17_Chr5g0422621	36.398	261	102	8	39	253	17	259	5.59e-36	133
MsaG025542	MtrunA17_Chr1g0198601	33.333	330	143	14	34	293	2	324	9.15e-36	133
MsaG025542	MtrunA17_Chr3g0137101	39.200	125	64	5	191	306	197	318	1.68e-14	74.3
MsaG025542	MtrunA17_Chr2g0291021	55.714	70	30	1	192	261	49	117	2.06e-14	73.2
MsaG025542	MtrunA17_Chr7g0254981	63.636	55	20	0	192	246	50	104	2.99e-14	72.0
MsaG025542	MtrunA17_Chr5g0417521	60.000	55	22	0	192	246	15	69	3.92e-14	72.4
MsaG025542	MtrunA17_Chr1g0197171	58.621	58	24	0	189	246	41	98	1.37e-13	70.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025542	AT4G37180.1	40.055	362	156	11	6	329	12	350	1.92e-60	197
MsaG025542	AT4G37180.2	39.295	369	156	12	6	329	12	357	1.46e-57	190
MsaG025542	AT4G37180.3	43.985	266	103	8	6	242	12	260	1.47e-51	172
MsaG025542	AT2G03500.1	38.112	286	133	8	9	250	6	291	1.73e-42	152
MsaG025542	AT1G68670.1	35.461	282	118	7	31	256	6	279	2.77e-40	145
MsaG025542	AT1G25550.1	35.636	275	116	7	31	256	10	272	4.50e-40	144
MsaG025542	AT1G49560.1	56.154	130	37	4	180	290	171	299	6.79e-35	130
MsaG025542	AT1G25550.2	34.483	261	111	6	31	242	10	259	1.13e-33	125
MsaG025542	AT1G13300.1	85.000	60	9	0	191	250	180	239	3.98e-29	115
MsaG025542	AT1G49560.2	70.833	72	12	1	180	242	171	242	4.18e-26	105

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Find 72 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTGGGTTGATGAAAA+TGG	0.274484	5:-9732038	MsaT025542.1:CDS
AAAATTAGTTACCATCATTA+AGG	0.265474	5:+9732177	None:intergenic
AACCTTTGCAGCAGAGTTCC+AGG	0.347695	5:-9731617	MsaT025542.1:CDS
AAGAGTGGTGATGGTAGTTC+TGG	0.342533	5:-9731687	MsaT025542.1:CDS
AAGATGAGCAATCAGATTGC+AGG	0.425444	5:-9730580	MsaT025542.1:CDS
AAGCCATGGAAGAAACAGTA+TGG	0.440126	5:-9730609	MsaT025542.1:CDS
AAGTTTCTGAGACATGTCGT+TGG	0.646359	5:+9732289	None:intergenic
AATGGTGGAGGGGTATTTAT+GGG	0.317942	5:-9731789	MsaT025542.1:CDS
AATTAGGGAAAAGATGCAAG+TGG	0.615575	5:-9730983	MsaT025542.1:CDS
ACAAAGGAGGTGCTGGTCGT+CGG	0.559002	5:-9731592	MsaT025542.1:CDS

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	9730538	9732396	9730538	ID=MsaG025542
Chr5	mRNA	9730538	9732396	9730538	ID=MsaT025542.1;Parent=MsaG025542
Chr5	exon	9730538	9730813	9730538	ID=MsaT025542.1.exon5;Parent=MsaT025542.1
Chr5	CDS	9730538	9730813	9730538	ID=cds.MsaT025542.1;Parent=MsaT025542.1
Chr5	exon	9730945	9731021	9730945	ID=MsaT025542.1.exon4;Parent=MsaT025542.1
Chr5	CDS	9730945	9731021	9730945	ID=cds.MsaT025542.1;Parent=MsaT025542.1
Chr5	exon	9731541	9731853	9731541	ID=MsaT025542.1.exon3;Parent=MsaT025542.1
Chr5	CDS	9731541	9731853	9731541	ID=cds.MsaT025542.1;Parent=MsaT025542.1
Chr5	exon	9731961	9732088	9731961	ID=MsaT025542.1.exon2;Parent=MsaT025542.1
Chr5	CDS	9731961	9732088	9731961	ID=cds.MsaT025542.1;Parent=MsaT025542.1
Chr5	exon	9732189	9732396	9732189	ID=MsaT025542.1.exon1;Parent=MsaT025542.1
Chr5	CDS	9732189	9732396	9732189	ID=cds.MsaT025542.1;Parent=MsaT025542.1

Gene Sequence

>MsaT025542.1

ATGGAACAACAACAATTGAACTTAGATTTGACTTTAGCCTCTGTTCCAAAAACCGTTTCTCATTTCCTCAACAACGTTGTTCAAACCAACGACATGTCTCAGAAACTTTCAATGCTTGATGACTTAGTTCATAGCTTGGAAGAAGAAATGAAGAAAGTTCTTGCTTTCAAACGTGAACTCCCTCTTTCCATACTCCTCCTTAATGATGCTATAGCAAGATTGAATGATGAAAAAGAAAAAGTTGGGTTGATGAAAATGGAAGATTTGAAAGAAAACTGTGATAATAAGAAGAATTGGATGAGTTCAGCTCAGCTATGGACTAATCAAACAAAAACGAACAATGAAGGTGATGATGATAGGATTGTGTTACAGAAGAGTAATGGTGGAGGGGTATTTATGGGATTTAATGAAAATCTGTTGAAGGAGGTTTCACAAGCTAAGAGCTTTAGTTTGGTGTCTGAAGTGAGTCATGGAAATTCAAAGAGTGGTGATGGTAGTTCTGGATCGTCTTTGTTGCGTGTTGAGATACAGAATCAACCTCAGCCGCCGCAACCTTTGCAGCAGAGTTCCAGGAAACAAAGGAGGTGCTGGTCGTCGGAGCTTCATCGCCGATTTGTTGATGCACTTCAACAGCTTGGTGGAGCACATGCAGCCACTCCTAAGCAAATTAGGGAAAAGATGCAAGTGGATGGATTAACAAATGATGAAGTGAAAAGCCATTTGCAAAAGTATAGGCTTCATGTTAGAAGATTCCCAGTTTCGTCAATTCAGGAGGCTAATAAGTTAGCCTTGTATATGGCTCAAGATCAATGTGAAGAAGATACATCAAAGGGAAACTTCTCAGAGTCTGTTTCTCCACAGGGTCCTCTTACTCCTCTTCTCCTTGGTGGATCTGTTCAAGGTCTTTCAAGCCATGGAAGAAACAGTATGGATGCTGAAGATGAGCAATCAGATTGCAGGAATTGGAAAAGTGATCAGGAGCAGCAGCATGAAGCTGAATAA

Protein sequence

>MsaT025542.1

MEQQQLNLDLTLASVPKTVSHFLNNVVQTNDMSQKLSMLDDLVHSLEEEMKKVLAFKRELPLSILLLNDAIARLNDEKEKVGLMKMEDLKENCDNKKNWMSSAQLWTNQTKTNNEGDDDRIVLQKSNGGGVFMGFNENLLKEVSQAKSFSLVSEVSHGNSKSGDGSSGSSLLRVEIQNQPQPPQPLQQSSRKQRRCWSSELHRRFVDALQQLGGAHAATPKQIREKMQVDGLTNDEVKSHLQKYRLHVRRFPVSSIQEANKLALYMAQDQCEEDTSKGNFSESVSPQGPLTPLLLGGSVQGLSSHGRNSMDAEDEQSDCRNWKSDQEQQHEAE*