AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG025588

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025588 MtrunA17_Chr5g0403291 77.996 459 50 2 125 539 1 452 0.0 727
MsaG025588 MtrunA17_Chr5g0402671 75.803 467 100 4 1 461 1 460 0.0 706
MsaG025588 MtrunA17_Chr5g0402771 70.664 467 131 1 1 461 1 467 0.0 657
MsaG025588 MtrunA17_Chr5g0402721 65.227 463 144 4 1 461 1 448 0.0 572
MsaG025588 MtrunA17_Chr5g0402681 63.715 463 150 6 1 461 1 447 0.0 554
MsaG025588 MtrunA17_Chr8g0373781 75.325 231 43 3 467 692 157 378 1.69e-120 370
MsaG025588 MtrunA17_Chr5g0403261 74.528 212 19 1 468 644 155 366 4.50e-109 335
MsaG025588 MtrunA17_Chr5g0402761 59.868 304 99 6 1 296 1 289 2.18e-106 325
MsaG025588 MtrunA17_Chr1g0154501 62.667 225 69 2 468 688 108 321 1.25e-99 314
MsaG025588 MtrunA17_Chr8g0374551 41.314 472 252 10 1 461 1 458 1.39e-97 318
MsaG025588 MtrunA17_Chr8g0374301 40.295 474 255 14 8 459 4 471 1.27e-96 316
MsaG025588 MtrunA17_Chr8g0374561 40.890 472 262 8 1 461 1 466 2.20e-95 312
MsaG025588 MtrunA17_Chr8g0374461 53.049 328 145 5 149 468 94 420 4.15e-95 311
MsaG025588 MtrunA17_Chr8g0374291 41.685 463 254 11 8 459 7 464 9.77e-94 308
MsaG025588 MtrunA17_Chr8g0374471 43.653 449 229 7 14 459 16 443 1.89e-92 305
MsaG025588 MtrunA17_Chr8g0374541 43.497 469 239 11 1 459 1 453 3.22e-90 299
MsaG025588 MtrunA17_Chr8g0374351 50.888 338 155 6 140 468 69 404 3.81e-90 297
MsaG025588 MtrunA17_Chr8g0374511 45.735 422 180 10 42 459 43 419 5.94e-89 295
MsaG025588 MtrunA17_Chr8g0374531 42.826 453 226 10 16 459 17 445 4.09e-87 290
MsaG025588 MtrunA17_Chr5g0436051 57.018 228 84 3 469 692 253 470 4.94e-85 280
MsaG025588 MtrunA17_Chr3g0112601 57.921 202 81 1 469 666 143 344 7.07e-81 266
MsaG025588 MtrunA17_Chr5g0402701 51.014 296 129 2 1 293 1 283 2.19e-75 251
MsaG025588 MtrunA17_Chr5g0403301 69.481 154 17 1 539 692 32 155 2.79e-64 215
MsaG025588 MtrunA17_Chr1g0153981 27.237 503 302 16 11 471 9 489 8.83e-40 157
MsaG025588 MtrunA17_Chr5g0403281 100.000 69 0 0 1 69 1 69 1.37e-39 140
MsaG025588 MtrunA17_Chr1g0153991 27.865 445 269 13 62 471 59 486 2.54e-34 140
MsaG025588 MtrunA17_Chr5g0402751 65.306 98 32 2 344 441 88 183 4.41e-33 125
MsaG025588 MtrunA17_Chr5g0402751 68.421 76 23 1 133 207 12 87 6.71e-24 99.8
MsaG025588 MtrunA17_Chr1g0159111 23.246 456 278 16 12 428 5 427 4.51e-16 82.4
MsaG025588 MtrunA17_Chr1g0189351 27.928 333 208 13 157 462 167 494 5.96e-15 79.0
MsaG025588 MtrunA17_Chr3g0105571 30.503 318 192 13 126 423 57 365 7.19e-15 78.2
MsaG025588 MtrunA17_Chr8g0343631 27.211 294 177 11 137 415 191 462 1.33e-14 77.8
MsaG025588 MtrunA17_Chr5g0439121 25.879 313 187 8 170 456 161 454 1.98e-14 77.8
MsaG025588 MtrunA17_Chr3g0105551 30.818 318 191 13 126 423 103 411 3.29e-14 77.0
MsaG025588 MtrunA17_Chr1g0189271 28.013 307 193 12 157 440 7 308 5.59e-14 73.6
MsaG025588 MtrunA17_Chr8g0343611 24.573 293 186 9 137 415 6 277 6.07e-14 75.1
MsaG025588 MtrunA17_Chr1g0160951 30.337 267 158 8 173 423 59 313 1.58e-13 74.7
MsaG025588 MtrunA17_Chr3g0107211 30.303 264 163 9 175 423 162 419 8.42e-13 72.0
MsaG025588 MtrunA17_Chr8g0374491 46.591 88 45 2 373 459 4 90 1.59e-12 70.5
MsaG025588 MtrunA17_Chr5g0409971 28.909 339 199 17 157 464 158 485 7.78e-12 68.9
MsaG025588 MtrunA17_Chr5g0410021 28.698 338 202 16 157 464 154 482 9.12e-12 68.6
MsaG025588 MtrunA17_Chr5g0409991 28.402 338 198 17 157 464 157 480 9.95e-12 68.6
MsaG025588 MtrunA17_Chr3g0097631 27.338 278 175 11 188 446 1 270 1.59e-11 67.0
MsaG025588 MtrunA17_Chr3g0081941 23.362 351 225 14 55 376 25 360 1.63e-11 68.2
MsaG025588 MtrunA17_Chr8g0360991 25.092 271 168 10 173 423 163 418 1.86e-11 67.8
MsaG025588 MtrunA17_Chr3g0107181 23.881 469 280 18 126 529 95 551 1.89e-11 67.8
MsaG025588 MtrunA17_Chr3g0101221 27.405 343 192 16 158 462 175 498 2.50e-11 67.4
MsaG025588 MtrunA17_Chr3g0100971 30.612 294 163 17 157 421 167 448 2.91e-11 67.0
MsaG025588 MtrunA17_Chr2g0313461 26.336 262 169 11 131 377 118 370 3.37e-11 67.0
MsaG025588 MtrunA17_Chr3g0100761 29.010 293 169 15 157 421 127 408 4.04e-11 66.2
MsaG025588 MtrunA17_Chr3g0101251 26.667 285 159 13 149 418 160 409 6.96e-11 65.9
MsaG025588 MtrunA17_Chr3g0100831 28.028 289 172 16 157 421 170 446 7.68e-11 65.9
MsaG025588 MtrunA17_Chr3g0099591 24.151 265 186 7 169 423 161 420 7.71e-11 65.9
MsaG025588 MtrunA17_Chr3g0101181 28.028 289 171 14 158 421 180 456 8.27e-11 65.5
MsaG025588 MtrunA17_Chr3g0101331 27.083 336 205 16 157 458 172 501 9.92e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025588 AT4G36860.2 72.926 229 49 2 468 692 187 406 2.15e-118 362
MsaG025588 AT4G36860.1 72.926 229 49 2 468 692 187 406 8.24e-118 364
MsaG025588 AT4G36860.3 72.926 229 49 2 468 692 211 430 2.00e-117 364
MsaG025588 AT1G19270.1 67.980 203 61 1 468 666 169 371 1.45e-102 324
MsaG025588 AT1G19270.2 67.980 203 61 1 468 666 169 371 1.45e-102 324
MsaG025588 AT2G39830.4 55.702 228 87 3 469 692 160 377 1.74e-84 273
MsaG025588 AT2G39830.3 55.702 228 87 3 469 692 151 368 3.69e-84 275
MsaG025588 AT2G39830.1 55.702 228 87 3 469 692 160 377 5.14e-84 275
MsaG025588 AT2G39830.2 55.702 228 87 3 469 692 160 377 6.37e-84 275
MsaG025588 AT5G66620.2 46.903 226 102 5 470 692 276 486 6.30e-62 215
MsaG025588 AT5G66620.1 46.903 226 102 5 470 692 285 495 3.80e-61 216
MsaG025588 AT5G66620.3 46.903 226 102 5 470 692 276 486 8.10e-61 215
MsaG025588 AT5G66620.4 46.903 226 102 5 470 692 276 486 8.10e-61 215
MsaG025588 AT5G66900.3 32.403 466 296 12 5 459 55 512 3.67e-58 207
MsaG025588 AT5G66610.1 39.414 307 160 8 370 666 103 393 1.22e-57 205
MsaG025588 AT5G66610.3 43.077 260 128 6 417 666 17 266 1.41e-57 201
MsaG025588 AT5G66610.2 39.414 307 160 8 370 666 103 393 1.45e-57 205
MsaG025588 AT5G66900.2 32.403 466 296 12 5 459 55 512 8.01e-57 208
MsaG025588 AT5G66900.1 32.403 466 296 12 5 459 7 464 8.32e-57 207
MsaG025588 AT5G17890.1 48.768 203 97 3 471 667 1240 1441 1.24e-52 197
MsaG025588 AT5G66910.1 30.947 475 301 10 7 465 12 475 2.22e-50 189
MsaG025588 AT5G66630.1 44.279 201 100 6 470 667 346 537 4.05e-48 181
MsaG025588 AT5G66630.1 33.754 317 203 5 1 312 6 320 4.35e-46 175
MsaG025588 AT5G66640.1 44.565 184 95 3 488 666 89 270 1.21e-44 166
MsaG025588 AT5G66640.7 44.565 184 95 3 488 666 89 270 1.21e-44 166
MsaG025588 AT5G66640.6 44.565 184 95 3 488 666 121 302 2.91e-44 166
MsaG025588 AT5G66640.2 44.565 184 95 3 488 666 120 301 3.06e-44 166
MsaG025588 AT5G66640.5 44.565 184 95 3 488 666 121 302 3.92e-44 166
MsaG025588 AT5G66640.4 44.565 184 95 3 488 666 120 301 4.37e-44 166
MsaG025588 AT5G66640.3 48.052 154 75 2 518 666 10 163 5.45e-42 156
MsaG025588 AT5G04720.1 26.543 486 307 15 1 459 1 463 1.97e-31 131
MsaG025588 AT5G47280.1 28.920 287 185 7 176 459 5 275 8.06e-28 119
MsaG025588 AT4G33300.2 26.096 479 305 13 10 458 8 467 1.42e-27 119
MsaG025588 AT4G33300.1 26.096 479 305 13 10 458 8 467 1.42e-27 119
MsaG025588 AT1G33560.1 26.214 412 239 15 73 461 73 442 3.93e-26 114
MsaG025588 AT1G12280.1 28.571 287 183 8 156 428 152 430 2.15e-17 87.4
MsaG025588 AT1G12220.3 28.125 288 184 9 156 428 31 310 2.97e-16 83.6
MsaG025588 AT1G12220.1 28.125 288 184 9 156 428 152 431 3.20e-16 83.6
MsaG025588 AT1G12220.2 28.125 288 184 9 156 428 152 431 3.20e-16 83.6
MsaG025588 AT1G59620.2 27.852 298 187 13 145 421 11 301 1.88e-15 80.9
MsaG025588 AT1G61310.1 27.108 332 220 12 156 470 151 477 1.10e-13 75.5
MsaG025588 AT1G63350.4 28.826 281 173 12 158 421 150 420 1.16e-13 75.1
MsaG025588 AT1G63350.3 28.826 281 173 12 158 421 150 420 1.31e-13 75.1
MsaG025588 AT1G63350.2 28.826 281 173 12 158 421 150 420 1.31e-13 75.1
MsaG025588 AT1G63350.1 28.826 281 173 12 158 421 150 420 1.37e-13 75.1
MsaG025588 AT1G12210.1 27.500 280 181 8 156 421 152 423 2.17e-13 74.3
MsaG025588 AT1G12210.3 27.500 280 181 8 156 421 110 381 2.30e-13 74.3
MsaG025588 AT1G12210.2 27.500 280 181 8 156 421 110 381 2.30e-13 74.3
MsaG025588 AT1G61190.4 26.644 289 186 11 156 428 150 428 5.98e-13 72.8
MsaG025588 AT1G61190.2 26.644 289 186 11 156 428 150 428 6.96e-13 72.8
MsaG025588 AT1G61190.3 26.644 289 186 11 156 428 150 428 6.96e-13 72.8
MsaG025588 AT1G61190.1 26.644 289 186 11 156 428 150 428 7.92e-13 72.8
MsaG025588 AT1G61180.1 28.169 284 188 11 156 428 149 427 2.49e-12 70.9
MsaG025588 AT1G61180.2 28.169 284 188 11 156 428 149 427 3.75e-12 70.5
MsaG025588 AT1G61300.2 27.740 292 194 11 150 428 29 316 6.66e-12 69.3
MsaG025588 AT1G61300.1 27.740 292 194 11 150 428 29 316 8.13e-12 69.3
MsaG025588 AT1G58400.3 25.746 268 172 11 177 423 181 442 5.81e-11 66.6
MsaG025588 AT1G58400.2 25.746 268 172 11 177 423 181 442 5.81e-11 66.6

Find 155 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTTAGAGTTCATCCTTTC+TGG 0.143060 5:+10257316 MsaT025588.1:CDS
TATGACATTAATCAAATTTC+TGG 0.159337 5:-10255823 None:intergenic
CATCGACGGGTCAGTTTATA+AGG 0.209815 5:-10258137 None:intergenic
TGAAGGTTTAAATATGAAAA+TGG 0.245113 5:+10257723 MsaT025588.1:CDS
GCCCTCTAGTTTCAGGTAAA+TGG 0.252523 5:-10257919 None:intergenic
GTGTCTTGACTCGGCAATTA+TGG 0.256992 5:+10257654 MsaT025588.1:CDS
AATGTCAGATTATAAAATTT+TGG 0.265234 5:+10254917 MsaT025588.1:CDS
TTTGGAAGCTTTGGCTCCTT+TGG 0.266951 5:+10253126 MsaT025588.1:CDS
GGGGCAAGTTCCTATGCTTT+TGG 0.268355 5:+10257747 MsaT025588.1:CDS
GGGTTCACCGCTGGTGATTC+AGG 0.274549 5:+10255512 MsaT025588.1:CDS
CATTGAAACTTTGGAAGCTT+TGG 0.286764 5:+10253117 MsaT025588.1:CDS
TCAACAAGGCCTTCTGAGTT+AGG 0.306709 5:-10254879 None:intergenic
TTGTTACCTTCAACAAAGTT+TGG 0.309146 5:+10255638 MsaT025588.1:CDS
TGAGAATAGAAGGGATTTGA+TGG 0.317102 5:+10253357 MsaT025588.1:CDS
CCCATGCATACAGGCTCTTC+AGG 0.325696 5:-10253469 None:intergenic
ACAGGAATCCTCTGGTCTTC+AGG 0.327301 5:-10255714 None:intergenic
AGTTTGATAGGAATCAAATA+AGG 0.327311 5:+10253431 MsaT025588.1:CDS
CATAAAATTCTAGTATTTCT+CGG 0.334425 5:-10257702 None:intergenic
AGAAATTTGATTAATGTCAT+AGG 0.338056 5:+10255825 MsaT025588.1:CDS
TCAACAAAGTTTGGATATAT+CGG 0.360110 5:+10255647 MsaT025588.1:CDS
CTATTACTACCTTTGATTTG+TGG 0.365008 5:-10253613 None:intergenic
CTGGTGATTCAGGTTATTGC+TGG 0.374523 5:+10255522 MsaT025588.1:CDS
TGAAACCAGCATTGAAACTT+TGG 0.382289 5:+10253108 MsaT025588.1:CDS
AAGCAGAGCCCTCTAGTTTC+AGG 0.382892 5:-10257926 None:intergenic
CTTTCTTCTTAAGCTTTCCT+TGG 0.383446 5:-10253294 None:intergenic
ATGTTCAAGTTGAGAATAGA+AGG 0.387869 5:+10253347 MsaT025588.1:CDS
TAAGTTGCATGGGAGGTGTC+TGG 0.392885 5:+10256501 MsaT025588.1:CDS
TGAAAATGGATCAGTTTGAT+AGG 0.393405 5:+10253419 MsaT025588.1:CDS
GAATGGTTTCAACGATTTGT+TGG 0.393705 5:+10253159 MsaT025588.1:CDS
TTCTTGCAAACATCGCATCT+TGG 0.397900 5:-10256809 None:intergenic
TCTCCCTGTAGCAGGACTTA+TGG 0.399080 5:-10256778 None:intergenic
TTGACTGGTTTGAGTGGATC+TGG 0.404456 5:+10253556 MsaT025588.1:CDS
AGTTCTGCCGGCAGATCAAT+GGG 0.404875 5:-10255744 None:intergenic
GTTTGATAGGAATCAAATAA+GGG 0.407774 5:+10253432 MsaT025588.1:CDS
CTCTTGCTAAGAAGCTCTGC+TGG 0.410471 5:+10253587 MsaT025588.1:CDS
TCTTGCAAACATCGCATCTT+GGG 0.413124 5:-10256808 None:intergenic
CAGTTCTGCCGGCAGATCAA+TGG 0.419863 5:-10255745 None:intergenic
TGCACAATGTTCTTCAAGTT+GGG 0.421332 5:-10254726 None:intergenic
AATGGTTTCAACGATTTGTT+GGG 0.430601 5:+10253160 MsaT025588.1:CDS
GCAGCACATACATGCCTGAT+AGG 0.436491 5:+10255048 MsaT025588.1:CDS
ATATATCCAAACTTTGTTGA+AGG 0.447490 5:-10255644 None:intergenic
CTGCACAATGTTCTTCAAGT+TGG 0.449800 5:-10254727 None:intergenic
TGCCGTCAAGCTCATGAGTT+GGG 0.454821 5:-10257353 None:intergenic
CAAGTGGATCCAACTCGAAT+GGG 0.458395 5:-10254974 None:intergenic
CTTTCACAGCTACAACAACT+TGG 0.459035 5:-10257378 None:intergenic
CCTTGGTAACGTGGGAAAGA+AGG 0.460904 5:-10253277 None:intergenic
AAAAGAGTGCTTCATGGATA+CGG 0.465073 5:+10255683 MsaT025588.1:CDS
AGCTTCCAAAGTTTCAATGC+TGG 0.465355 5:-10253113 None:intergenic
ACAGATAGTGAAAGGCTGTA+AGG 0.465465 5:+10255491 MsaT025588.1:intron
ACGGGTCAGTTTATAAGGCT+TGG 0.467747 5:-10258132 None:intergenic
ATTTGAACACTGTGGATGTC+GGG 0.469451 5:+10254755 MsaT025588.1:CDS
CTAACCTCAAAATCAGTGAT+TGG 0.470214 5:-10256560 None:intergenic
AATCTCTCTATCTCTTCTCT+TGG 0.472775 5:-10253187 None:intergenic
TCACGGACAAGATTCCTATC+AGG 0.473862 5:-10255062 None:intergenic
CTATTAAGTGGAGGTGCTGT+TGG 0.475176 5:+10253013 MsaT025588.1:CDS
GTGATGCTTCTCCCTGTAGC+AGG 0.482197 5:-10256786 None:intergenic
GGTGATTAAATCGTTGTCAA+TGG 0.485925 5:-10255802 None:intergenic
TCAATCTTCAACTTATTCAA+AGG 0.487246 5:-10253502 None:intergenic
CCTTTGGTTGCAGAAATGAA+TGG 0.490038 5:+10253142 MsaT025588.1:CDS
AAATCCAATACTATTAGTCT+TGG 0.490727 5:+10254845 MsaT025588.1:CDS
AAGCTTAAGAAGAAAGATGA+AGG 0.493473 5:+10253301 MsaT025588.1:CDS
AAGATTGAATTGATGAAAGA+TGG 0.496149 5:+10253517 MsaT025588.1:CDS
TGAGAAGTGGCAAAATATGA+AGG 0.496940 5:+10255566 MsaT025588.1:CDS
GACGATGTCATTAACCGATT+GGG 0.505523 5:+10254798 MsaT025588.1:CDS
TTCAATGCTGGTTTCAAGTG+TGG 0.506107 5:-10253101 None:intergenic
TCATCCATTCCCATGCATAC+AGG 0.511272 5:-10253478 None:intergenic
GTCAGTTTATAAGGCTTGGT+TGG 0.513453 5:-10258128 None:intergenic
AAAACTTGAATTTGGAAGCA+AGG 0.513888 5:-10257983 None:intergenic
TCAATGCTGGTTTCAAGTGT+GGG 0.517607 5:-10253100 None:intergenic
TCCTGAAGAGCCTGTATGCA+TGG 0.520250 5:+10253468 MsaT025588.1:CDS
TCGGTTAATGACATCGTCAT+CGG 0.520259 5:-10254793 None:intergenic
TGACGATGTCATTAACCGAT+TGG 0.520859 5:+10254797 MsaT025588.1:CDS
TGATTAATGTCATAGGTTCA+AGG 0.527019 5:+10255832 MsaT025588.1:CDS
ACCGATTGGGTCTTCTGTTG+AGG 0.528164 5:+10254811 MsaT025588.1:CDS
TTTGTATACAAAATCTGTGC+GGG 0.529190 5:+10256446 MsaT025588.1:CDS
TCATCCAAGACTAATAGTAT+TGG 0.531730 5:-10254849 None:intergenic
CAGATAGTGAAAGGCTGTAA+GGG 0.531994 5:+10255492 MsaT025588.1:intron
AAATCTTCCATGGCAAATCT+CGG 0.533441 5:-10256477 None:intergenic
GCAGAGCTTCTTAGCAAGAG+TGG 0.533470 5:-10253584 None:intergenic
TTAAGTGGAGGTGCTGTTGG+AGG 0.533913 5:+10253016 MsaT025588.1:CDS
TCGTCATCGGTTTGAAACTC+AGG 0.534795 5:-10254780 None:intergenic
TATTTGAACACTGTGGATGT+CGG 0.535776 5:+10254754 MsaT025588.1:CDS
CTTACCATAAGTCCTGCTAC+AGG 0.538626 5:+10256774 MsaT025588.1:CDS
TCAAGTGGATCCAACTCGAA+TGG 0.539353 5:-10254975 None:intergenic
ATTTGGTACCCCATTCGAGT+TGG 0.540077 5:+10254965 MsaT025588.1:CDS
AGCACTAAAAGAGGCCATAG+AGG 0.541584 5:+10257780 MsaT025588.1:CDS
TGCCCAACTCATGAGCTTGA+CGG 0.543153 5:+10257351 MsaT025588.1:CDS
GTATTTCTCGGTAGAGAGGT+TGG 0.543209 5:-10257690 None:intergenic
AAAGAGTGCTTCATGGATAC+GGG 0.546487 5:+10255684 MsaT025588.1:CDS
TTGTTGTAGCTGTGAAAGAA+TGG 0.548998 5:+10257383 MsaT025588.1:CDS
CAAGCATGGCAGACGAAGCA+TGG 0.548999 5:-10256530 None:intergenic
TGTTCAAGTTGAGAATAGAA+GGG 0.550405 5:+10253348 MsaT025588.1:CDS
GGTCTTCTGTTGAGGCAAGT+TGG 0.550993 5:+10254819 MsaT025588.1:CDS
CTGTAGCAGGACTTATGGTA+AGG 0.553104 5:-10256773 None:intergenic
GGGCAGTAACAGGAATCCTC+TGG 0.559280 5:-10255722 None:intergenic
TGATTGGCAGATGGCAAGCA+TGG 0.564914 5:-10256544 None:intergenic
GTGCCGTCAAGCTCATGAGT+TGG 0.570183 5:-10257354 None:intergenic
TGCATGGCGGATCTATTAAG+TGG 0.570389 5:+10253001 None:intergenic
TCTAGTATTTCTCGGTAGAG+AGG 0.572580 5:-10257694 None:intergenic
TTAGAGAAACACATAAGAGA+AGG 0.572972 5:+10253208 MsaT025588.1:CDS
GGAAAGACAAGCACTAAAAG+AGG 0.575269 5:+10257771 MsaT025588.1:CDS
AGGCTGTAAGGGTTCACCGC+TGG 0.576228 5:+10255503 MsaT025588.1:CDS
GAATAGAAGGGATTTGATGG+AGG 0.577512 5:+10253360 MsaT025588.1:CDS
TTAAGCTTTCCTTGGTAACG+TGG 0.577842 5:-10253286 None:intergenic
GGTTTAAATATGAAAATGGA+GGG 0.578948 5:+10257727 MsaT025588.1:CDS
TCTGAGTTATGCAGTTCTGC+CGG 0.578981 5:-10255756 None:intergenic
CTCAGAAGAAGATGGTGCAA+AGG 0.581165 5:+10255773 MsaT025588.1:CDS
TGCAATGCCGAGATTTGCCA+TGG 0.581684 5:+10256470 MsaT025588.1:CDS
TGTCTTTCCACCAAAAGCAT+AGG 0.584605 5:-10257757 None:intergenic
CCATTCATTTCTGCAACCAA+AGG 0.585398 5:-10253142 None:intergenic
CCTTCTTTCCCACGTTACCA+AGG 0.586461 5:+10253277 MsaT025588.1:CDS
TTCTATGCACAGATAGTGAA+AGG 0.587153 5:+10255483 MsaT025588.1:intron
CTGCATAACTCAGAAGAAGA+TGG 0.590966 5:+10255765 MsaT025588.1:CDS
ACCATTTACCTGAAACTAGA+GGG 0.591314 5:+10257918 MsaT025588.1:CDS
GCCTCAACAGAAGACCCAAT+CGG 0.592079 5:-10254812 None:intergenic
AGGTTTAAATATGAAAATGG+AGG 0.594562 5:+10257726 MsaT025588.1:CDS
GAACTAACTGTCACCACCGT+AGG 0.595078 5:+10257956 MsaT025588.1:CDS
TGCTGGGATCCACAAATCAA+AGG 0.596374 5:+10253604 MsaT025588.1:CDS
AGAGCCTGTATGCATGGGAA+TGG 0.597515 5:+10253474 MsaT025588.1:CDS
CAAAATCAGTGATTGGCAGA+TGG 0.598645 5:-10256553 None:intergenic
TACCATTTACCTGAAACTAG+AGG 0.599700 5:+10257917 MsaT025588.1:CDS
TCTTGCTAAGAAGCTCTGCT+GGG 0.599802 5:+10253588 MsaT025588.1:CDS
TTTGATAGGAATCAAATAAG+GGG 0.602155 5:+10253433 MsaT025588.1:CDS
TAAGCTTTCCTTGGTAACGT+GGG 0.602459 5:-10253285 None:intergenic
GTAAGGACTATTGCTAGACA+TGG 0.605124 5:-10256756 None:intergenic
GATGAACTCTAAACTCAATG+AGG 0.605371 5:-10257308 None:intergenic
ACTGCATGATTACGATCAAG+TGG 0.605604 5:-10254990 None:intergenic
GATGTTAAGCCTAACTCAGA+AGG 0.607034 5:+10254870 MsaT025588.1:CDS
AACTAACTGTCACCACCGTA+GGG 0.609828 5:+10257957 MsaT025588.1:CDS
AAAGCAAGAAGCTTACTCGA+TGG 0.613136 5:+10253248 MsaT025588.1:CDS
TTCTGCCGGCAGATCAATGG+GGG 0.614894 5:-10255742 None:intergenic
GCACTAAAAGAGGCCATAGA+GGG 0.619837 5:+10257781 MsaT025588.1:CDS
GGAATTTGAACTTCTCAACA+AGG 0.619958 5:-10254893 None:intergenic
AATCAAATAAGGGGTTTGTG+TGG 0.623843 5:+10253442 MsaT025588.1:CDS
GATTCAATTGAGCAAAGTGA+TGG 0.624063 5:-10255022 None:intergenic
ATGTCTTGAGTGTCTTGACT+CGG 0.625398 5:+10257645 MsaT025588.1:CDS
CCTGAAGAGCCTGTATGCAT+GGG 0.631595 5:+10253469 MsaT025588.1:CDS
GACTATTTCCTGAAGACCAG+AGG 0.631906 5:+10255706 MsaT025588.1:CDS
TTACCATAAGTCCTGCTACA+GGG 0.636079 5:+10256775 MsaT025588.1:CDS
AGCTTAAGAAGAAAGATGAA+GGG 0.648390 5:+10253302 MsaT025588.1:CDS
GTTTAAATATGAAAATGGAG+GGG 0.650220 5:+10257728 MsaT025588.1:CDS
TGGTTATCATGTCTGTGACT+CGG 0.666269 5:-10258108 None:intergenic
TTTAAGAAGATCAATTATGG+GGG 0.667690 5:+10258078 MsaT025588.1:CDS
ATTTGTATACAAAATCTGTG+CGG 0.668443 5:+10256445 MsaT025588.1:CDS
AGTGATATTAGACTCAAGAA+CGG 0.673387 5:-10255607 None:intergenic
AAGTGGATCCAACTCGAATG+GGG 0.677606 5:-10254973 None:intergenic
TTGGAAGCAAGGAATCCCTA+CGG 0.678547 5:-10257972 None:intergenic
GCATGGCAGACGAAGCATGG+TGG 0.685519 5:-10256527 None:intergenic
GTTCTGCCGGCAGATCAATG+GGG 0.689050 5:-10255743 None:intergenic
ATGGCGGATCTATTAAGTGG+AGG 0.698581 5:+10253004 MsaT025588.1:CDS
TGATAGGAATCTTGTCCGTG+AGG 0.725852 5:+10255064 MsaT025588.1:CDS
TTGTAGCTGTGAAAGAATGG+AGG 0.727987 5:+10257386 MsaT025588.1:CDS
GCAATAACCTGAATCACCAG+CGG 0.734403 5:-10255519 None:intergenic
GAAGCAAGGAATCCCTACGG+TGG 0.739116 5:-10257969 None:intergenic
CAGACACTATTTGAACACTG+TGG 0.785580 5:+10254747 MsaT025588.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 10253004 10258144 10253004 ID=MsaG025588
Chr5 mRNA 10253004 10258144 10253004 ID=MsaT025588.1;Parent=MsaG025588
Chr5 exon 10253004 10253625 10253004 ID=MsaT025588.1.exon1;Parent=MsaT025588.1
Chr5 CDS 10253004 10253625 10253004 ID=cds.MsaT025588.1;Parent=MsaT025588.1
Chr5 exon 10254682 10255085 10254682 ID=MsaT025588.1.exon2;Parent=MsaT025588.1
Chr5 CDS 10254682 10255085 10254682 ID=cds.MsaT025588.1;Parent=MsaT025588.1
Chr5 exon 10255495 10255853 10255495 ID=MsaT025588.1.exon3;Parent=MsaT025588.1
Chr5 CDS 10255495 10255853 10255495 ID=cds.MsaT025588.1;Parent=MsaT025588.1
Chr5 exon 10256436 10256577 10256436 ID=MsaT025588.1.exon4;Parent=MsaT025588.1
Chr5 CDS 10256436 10256577 10256436 ID=cds.MsaT025588.1;Parent=MsaT025588.1
Chr5 exon 10256752 10256839 10256752 ID=MsaT025588.1.exon5;Parent=MsaT025588.1
Chr5 CDS 10256752 10256839 10256752 ID=cds.MsaT025588.1;Parent=MsaT025588.1
Chr5 exon 10257304 10257407 10257304 ID=MsaT025588.1.exon6;Parent=MsaT025588.1
Chr5 CDS 10257304 10257407 10257304 ID=cds.MsaT025588.1;Parent=MsaT025588.1
Chr5 exon 10257601 10257813 10257601 ID=MsaT025588.1.exon7;Parent=MsaT025588.1
Chr5 CDS 10257601 10257813 10257601 ID=cds.MsaT025588.1;Parent=MsaT025588.1
Chr5 exon 10257911 10258000 10257911 ID=MsaT025588.1.exon8;Parent=MsaT025588.1
Chr5 CDS 10257911 10258000 10257911 ID=cds.MsaT025588.1;Parent=MsaT025588.1
Chr5 exon 10258076 10258144 10258076 ID=MsaT025588.1.exon9;Parent=MsaT025588.1
Chr5 CDS 10258076 10258144 10258076 ID=cds.MsaT025588.1;Parent=MsaT025588.1
Gene Sequence

>MsaT025588.1

ATGGCGGATCTATTAAGTGGAGGTGCTGTTGGAGGTGTGATGAGTGAAATATTGAAACAAGCTCTTCAAACAATAAAAAATGGTCGTGAATTCGGTCCCACACTTGAAACCAGCATTGAAACTTTGGAAGCTTTGGCTCCTTTGGTTGCAGAAATGAATGGTTTCAACGATTTGTTGGGTCGACCAAGAGAAGAGATAGAGAGATTAGAGAAACACATAAGAGAAGGTAAAGAGCTTGTGCAAAAAAGCAAGAAGCTTACTCGATGGAACTTTCCTTCTTTCCCACGTTACCAAGGAAAGCTTAAGAAGAAAGATGAAGGGCTAAAGAGACATTTGAATGTTAATGTTCAAGTTGAGAATAGAAGGGATTTGATGGAGGTTTTGACTAAGGTGAATGGAATTCTTGAGATTTTGATGAAAATGGATCAGTTTGATAGGAATCAAATAAGGGGTTTGTGTGGTGCTCCTGAAGAGCCTGTATGCATGGGAATGGATGAACCTTTGAATAAGTTGAAGATTGAATTGATGAAAGATGGTGTTTCTGTGCTTGTTTTGACTGGTTTGAGTGGATCTGGTAAAACCACTCTTGCTAAGAAGCTCTGCTGGGATCCACAAATCAAAGGCAAGTTTGGTGAAAATATCTTCCTTGTCACCGTCTCAAAAACTCCCAACTTGAAGAACATTGTGCAGACACTATTTGAACACTGTGGATGTCGGGTTCCTGAGTTTCAAACCGATGACGATGTCATTAACCGATTGGGTCTTCTGTTGAGGCAAGTTGGTAGAAATCCAATACTATTAGTCTTGGATGATGTTAAGCCTAACTCAGAAGGCCTTGTTGAGAAGTTCAAATTCCAAATGTCAGATTATAAAATTTTGGTGACGTCGAGGGTTGCTTTTAGAAGATTTGGTACCCCATTCGAGTTGGATCCACTTGATCGTAATCATGCAGTGTCTCTATTCCATCACTTTGCTCAATTGAATCACAGCAGCACATACATGCCTGATAGGAATCTTGTCCGTGAGATAGTGAAAGGCTGTAAGGGTTCACCGCTGGTGATTCAGGTTATTGCTGGATCACTATGCAAGCAGCCTTTTGAGAAGTGGCAAAATATGAAGGAACGTTTAAAGAGTAAAGCCGTTCTTGAGTCTAATATCACTGACTTGCTTTGTTACCTTCAACAAAGTTTGGATATATCGGACGATATCAATGAAAAAGAGTGCTTCATGGATACGGGACTATTTCCTGAAGACCAGAGGATTCCTGTTACTGCCCCCATTGATCTGCCGGCAGAACTGCATAACTCAGAAGAAGATGGTGCAAAGGCAATCGCCATTGACAACGATTTAATCACCAGAAATTTGATTAATGTCATAGGTTCAAGTTCTGAAAAATTTGTATACAAAATCTGTGCGGGATGCAATGCCGAGATTTGCCATGGAAGATTTTTAAGTTGCATGGGAGGTGTCTGGCATCCACCATGCTTCGTCTGCCATGCTTGCCATCTGCCAATCACTGATTTTGAGTTTTCCATGTCTAGCAATAGTCCTTACCATAAGTCCTGCTACAGGGAGAAGCATCACCCAAGATGCGATGTTTGCAAGAATTTTGTAACTGGCCTCATTGAGTTTAGAGTTCATCCTTTCTGGATTCAAAAGTACTGCCCAACTCATGAGCTTGACGGCACTCCAAGTTGTTGTAGCTGTGAAAGAATGGAGCCAAAGGATTCAAAATATCTTTTGCTTGATGATGGTCGAAAGCTATGTCTTGAGTGTCTTGACTCGGCAATTATGGATAATGATGAATGCCAACCTCTCTACCGAGAAATACTAGAATTTTATGAAGGTTTAAATATGAAAATGGAGGGGCAAGTTCCTATGCTTTTGGTGGAAAGACAAGCACTAAAAGAGGCCATAGAGGGAGAAAAGAATGGTCATTACCATTTACCTGAAACTAGAGGGCTCTGCTTGTCAGAAGAACTAACTGTCACCACCGTAGGGATTCCTTGCTTCCAAATTCAAATTTTAAGAAGATCAATTATGGGGGCAGGATACCGAGTCACAGACATGATAACCAACCAAGCCTTATAA
Protein sequence

>MsaT025588.1

MADLLSGGAVGGVMSEILKQALQTIKNGREFGPTLETSIETLEALAPLVAEMNGFNDLLGRPREEIERLEKHIREGKELVQKSKKLTRWNFPSFPRYQGKLKKKDEGLKRHLNVNVQVENRRDLMEVLTKVNGILEILMKMDQFDRNQIRGLCGAPEEPVCMGMDEPLNKLKIELMKDGVSVLVLTGLSGSGKTTLAKKLCWDPQIKGKFGENIFLVTVSKTPNLKNIVQTLFEHCGCRVPEFQTDDDVINRLGLLLRQVGRNPILLVLDDVKPNSEGLVEKFKFQMSDYKILVTSRVAFRRFGTPFELDPLDRNHAVSLFHHFAQLNHSSTYMPDRNLVREIVKGCKGSPLVIQVIAGSLCKQPFEKWQNMKERLKSKAVLESNITDLLCYLQQSLDISDDINEKECFMDTGLFPEDQRIPVTAPIDLPAELHNSEEDGAKAIAIDNDLITRNLINVIGSSSEKFVYKICAGCNAEICHGRFLSCMGGVWHPPCFVCHACHLPITDFEFSMSSNSPYHKSCYREKHHPRCDVCKNFVTGLIEFRVHPFWIQKYCPTHELDGTPSCCSCERMEPKDSKYLLLDDGRKLCLECLDSAIMDNDECQPLYREILEFYEGLNMKMEGQVPMLLVERQALKEAIEGEKNGHYHLPETRGLCLSEELTVTTVGIPCFQIQILRRSIMGAGYRVTDMITNQAL*