AlfalfaGEDB | MsaG025623

M. sativa ssp. caerulea / MsaG025623

KEGG: K09338
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0007275 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009889 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022622 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0048364 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00046.32

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025623	XP_003611946.1	89.640	222	15	2	1	215	1	221	4.88e-129	372

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025623	sp\|O23208\|ATB40_ARATH	53.302	212	91	7	7	215	10	216	1.19e-60	192

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025623	tr\|G7KC72\|G7KC72_MEDTR	89.640	222	15	2	1	215	1	221	2.33e-129	372

TFs/TRs:

Gene ID	Type	Classification
MsaG025623	TF	HB-other

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025623	MtrunA17_Chr5g0403691	89.640	222	15	2	1	215	1	221	4.49e-133	372
MsaG025623	MtrunA17_Chr5g0403701	81.690	213	33	2	1	213	1	207	1.01e-113	323
MsaG025623	MtrunA17_Chr4g0060031	44.275	131	58	3	34	149	67	197	2.28e-23	95.5
MsaG025623	MtrunA17_Chr6g0450991	49.107	112	46	3	54	157	67	175	3.16e-23	94.4
MsaG025623	MtrunA17_Chr3g0123691	60.811	74	29	0	54	127	60	133	8.93e-22	90.9
MsaG025623	MtrunA17_Chr8g0381741	52.475	101	39	1	54	145	54	154	1.76e-21	90.1
MsaG025623	MtrunA17_Chr6g0453021	50.495	101	50	0	43	143	85	185	7.14e-21	87.8
MsaG025623	MtrunA17_Chr7g0265521	34.857	175	90	4	48	205	57	224	7.99e-21	87.0
MsaG025623	MtrunA17_Chr8g0362751	46.087	115	54	3	54	160	89	203	2.77e-20	86.7
MsaG025623	MtrunA17_Chr8g0362151	43.966	116	58	2	46	160	69	178	3.05e-20	86.3
MsaG025623	MtrunA17_Chr1g0179581	51.807	83	40	0	54	136	28	110	5.84e-20	81.6
MsaG025623	MtrunA17_Chr5g0416391	42.609	115	61	1	46	160	69	178	8.44e-20	85.1
MsaG025623	MtrunA17_Chr5g0415941	59.155	71	29	0	54	124	87	157	1.02e-19	85.5
MsaG025623	MtrunA17_Chr3g0127511	50.000	92	39	1	56	140	40	131	4.48e-19	82.4
MsaG025623	MtrunA17_Chr8g0346251	44.954	109	53	2	58	164	45	148	2.30e-18	80.5
MsaG025623	MtrunA17_Chr3g0119291	40.141	142	72	3	51	179	56	197	7.90e-18	79.3
MsaG025623	MtrunA17_Chr7g0217111	47.778	90	47	0	54	143	80	169	1.37e-15	73.6
MsaG025623	MtrunA17_Chr2g0308611	35.616	146	88	2	34	173	180	325	6.86e-15	72.4
MsaG025623	MtrunA17_Chr4g0055851	42.727	110	57	2	39	143	106	214	3.43e-14	69.7
MsaG025623	MtrunA17_Chr8g0335131	45.263	95	47	1	54	143	146	240	1.21e-13	68.6
MsaG025623	MtrunA17_Chr4g0044091	53.731	67	31	0	54	120	65	131	2.18e-13	66.2
MsaG025623	MtrunA17_Chr4g0054421	45.263	95	47	1	54	143	127	221	3.05e-13	67.0
MsaG025623	MtrunA17_Chr1g0151971	33.775	151	93	2	54	197	130	280	2.32e-12	64.7
MsaG025623	MtrunA17_Chr1g0175651	38.053	113	65	1	34	141	134	246	2.43e-12	64.7
MsaG025623	MtrunA17_Chr5g0400781	45.263	95	47	1	54	143	123	217	3.01e-12	64.3
MsaG025623	MtrunA17_Chr7g0258831	37.500	112	65	1	35	141	141	252	4.71e-12	63.9
MsaG025623	MtrunA17_Chr5g0399491	43.158	95	49	1	54	143	136	230	6.36e-12	63.5
MsaG025623	MtrunA17_Chr3g0135331	43.836	73	41	0	52	124	143	215	2.82e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025623	AT4G36740.2	52.830	212	93	6	7	215	10	217	5.24e-62	192
MsaG025623	AT4G36740.1	53.302	212	91	7	7	215	10	216	1.21e-61	192
MsaG025623	AT2G18550.1	47.345	226	102	8	1	215	1	220	1.62e-61	191
MsaG025623	AT5G66700.1	45.333	225	104	7	5	215	9	228	5.52e-44	147
MsaG025623	AT3G01470.1	49.505	101	44	1	50	143	64	164	9.34e-24	95.9
MsaG025623	AT1G69780.1	38.816	152	83	3	2	143	23	174	1.02e-22	93.6
MsaG025623	AT4G40060.1	57.317	82	35	0	43	124	48	129	9.13e-22	90.9
MsaG025623	AT1G26960.1	44.355	124	64	1	54	177	71	189	1.48e-21	89.7
MsaG025623	AT2G22430.1	64.789	71	25	0	54	124	62	132	1.51e-21	90.9
MsaG025623	AT5G03790.1	50.000	94	46	1	46	139	70	162	5.63e-21	87.8
MsaG025623	AT5G65310.2	65.152	66	23	0	54	119	54	119	1.11e-20	88.2
MsaG025623	AT5G65310.1	58.108	74	31	0	46	119	64	137	1.17e-20	88.2
MsaG025623	AT5G03790.2	48.936	94	47	1	46	139	70	162	1.67e-20	85.5
MsaG025623	AT2G46680.2	47.748	111	51	1	48	151	26	136	1.02e-19	84.7
MsaG025623	AT2G36610.1	46.296	108	50	2	46	145	63	170	1.21e-19	83.2
MsaG025623	AT2G46680.1	40.945	127	68	1	48	174	26	145	2.41e-19	84.0
MsaG025623	AT3G01220.1	50.000	86	43	0	39	124	72	157	9.64e-19	82.8
MsaG025623	AT5G53980.1	44.444	99	55	0	45	143	2	100	1.58e-18	79.3
MsaG025623	AT5G15150.1	53.750	80	37	0	41	120	102	181	1.96e-18	82.4
MsaG025623	AT1G27045.5	57.353	68	29	0	52	119	23	90	9.02e-17	75.5
MsaG025623	AT1G27045.3	57.353	68	29	0	52	119	23	90	9.02e-17	75.5
MsaG025623	AT3G61890.1	40.336	119	65	1	57	169	33	151	9.19e-17	76.6
MsaG025623	AT1G27045.2	57.353	68	29	0	52	119	15	82	1.07e-16	75.1
MsaG025623	AT1G27045.1	57.353	68	29	0	52	119	66	133	1.27e-16	75.9
MsaG025623	AT1G27045.4	57.353	68	29	0	52	119	66	133	1.78e-16	75.9
MsaG025623	AT2G22800.1	45.161	93	46	1	54	141	113	205	1.78e-13	68.2
MsaG025623	AT4G16780.1	40.741	108	54	2	41	143	121	223	6.16e-13	66.6
MsaG025623	AT4G17460.1	38.760	129	72	3	45	166	126	254	1.57e-12	65.5
MsaG025623	AT4G37790.1	44.086	93	47	1	54	141	126	218	1.89e-12	65.1
MsaG025623	AT2G01430.1	37.008	127	65	2	54	179	139	251	5.70e-12	63.9
MsaG025623	AT5G47370.1	33.766	154	95	3	33	179	109	262	8.79e-12	63.5
MsaG025623	AT5G06710.1	39.048	105	64	0	35	139	171	275	1.81e-11	62.8
MsaG025623	AT3G60390.1	40.000	105	58	1	44	143	152	256	2.26e-11	62.4

Find 42 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCTTGTTGAGGAATTATT+TGG	0.121044	5:-10740963	None:intergenic
TCCACCTAGCTCTTCGATTC+TGG	0.214518	5:-10741288	None:intergenic
TGATGAACAGATGAAAATTT+TGG	0.274813	5:+10741168	MsaT025623.1:CDS
TAAGCATCATTTGGTCTTGA+TGG	0.320716	5:-10740909	None:intergenic
GAACCATACTGCAACTTGTC+TGG	0.324204	5:-10741266	None:intergenic
TTGTAGTAACATGTTTCATT+AGG	0.345702	5:-10742436	None:intergenic
CTACGCTTGCGTCTCGGCTT+TGG	0.362052	5:-10741082	None:intergenic
CTGCATGTTACCCTTTCAAT+TGG	0.385057	5:-10742307	None:intergenic
ATCCCAGACAAGTTGCAGTA+TGG	0.391231	5:+10741263	MsaT025623.1:CDS
TTCTTACTACGCTTGCGTCT+CGG	0.430545	5:-10741088	None:intergenic
ACCTTGTTGAGGAATTATTT+GGG	0.434434	5:-10740962	None:intergenic
AGAGGTATACCTTGGTTTCA+AGG	0.439510	5:-10741384	None:intergenic
ACTGAGACATAAGCATCATT+TGG	0.463969	5:-10740918	None:intergenic
CGTAGTAAGAAGAACAAAAG+TGG	0.482800	5:+10741100	MsaT025623.1:CDS
GATAGGCTTGCAATGGAGCT+TGG	0.487004	5:+10741235	MsaT025623.1:CDS
AAGGAGATACTTCTCAAGAA+TGG	0.497377	5:-10741365	None:intergenic
TAAACTTGAGTCAGAGAAGA+AGG	0.499532	5:+10741213	MsaT025623.1:CDS
GTCTCAGTCAGTGGAAGTTG+TGG	0.499926	5:+10742351	MsaT025623.1:CDS
GGTGAATTTGGTGTTGATAA+CGG	0.514478	5:+10742388	MsaT025623.1:CDS
ACACCAAATTCACCAAGAAA+AGG	0.517876	5:-10742379	None:intergenic
AACCATACTGCAACTTGTCT+GGG	0.517968	5:-10741265	None:intergenic
TTGAGTCAGAGAAGAAGGAT+AGG	0.537241	5:+10741218	MsaT025623.1:CDS
ATTTGGGTGTATTCACAAGC+GGG	0.547719	5:-10740946	None:intergenic
GCTTCGAGAGCCAATTGAAA+GGG	0.560533	5:+10742297	MsaT025623.1:CDS
CCAAATTCACCAAGAAAAGG+TGG	0.566129	5:-10742376	None:intergenic
GTTACTACAATGGCATGGAA+TGG	0.566673	5:+10742449	MsaT025623.1:CDS
TGCTTCGAGAGCCAATTGAA+AGG	0.569118	5:+10742296	MsaT025623.1:CDS
AACTTCCACTGACTGAGACA+TGG	0.581765	5:-10742347	None:intergenic
TTCATCCATGTCTCAGTCAG+TGG	0.582296	5:+10742342	MsaT025623.1:CDS
GAAGAAGGATAGGCTTGCAA+TGG	0.584642	5:+10741228	MsaT025623.1:CDS
AATGAAACATGTTACTACAA+TGG	0.596613	5:+10742439	MsaT025623.1:CDS
TGTATAGGTGTTGAAGCTAA+CGG	0.597602	5:+10742240	MsaT025623.1:intron
GGTTCCAGAATCGAAGAGCT+AGG	0.598892	5:+10741284	MsaT025623.1:CDS
TATTTGGGTGTATTCACAAG+CGG	0.604533	5:-10740947	None:intergenic
ACTTGATACTAACCTTGTTG+AGG	0.606044	5:-10740973	None:intergenic
GAGATACTTCTCAAGAATGG+TGG	0.612212	5:-10741362	None:intergenic
AACATGTTACTACAATGGCA+TGG	0.616964	5:+10742444	MsaT025623.1:CDS
GAAGTATCTCCTTGAAACCA+AGG	0.625666	5:+10741375	MsaT025623.1:CDS
CAAATTCACCAAGAAAAGGT+GGG	0.668413	5:-10742375	None:intergenic
TTTGGGTGTATTCACAAGCG+GGG	0.669434	5:-10740945	None:intergenic
ACCCAAATAATTCCTCAACA+AGG	0.682907	5:+10740961	MsaT025623.1:CDS
TCCAGAATCGAAGAGCTAGG+TGG	0.707809	5:+10741287	MsaT025623.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	10740904	10742489	10740904	ID=MsaG025623
Chr5	mRNA	10740904	10742489	10740904	ID=MsaT025623.1;Parent=MsaG025623
Chr5	exon	10740904	10740982	10740904	ID=MsaT025623.1.exon1;Parent=MsaT025623.1
Chr5	CDS	10740904	10740982	10740904	ID=cds.MsaT025623.1;Parent=MsaT025623.1
Chr5	exon	10741071	10741396	10741071	ID=MsaT025623.1.exon2;Parent=MsaT025623.1
Chr5	CDS	10741071	10741396	10741071	ID=cds.MsaT025623.1;Parent=MsaT025623.1
Chr5	exon	10742247	10742489	10742247	ID=MsaT025623.1.exon3;Parent=MsaT025623.1
Chr5	CDS	10742247	10742489	10742247	ID=cds.MsaT025623.1;Parent=MsaT025623.1

Gene Sequence

>MsaT025623.1

ATGAACCATCAAGACCAAATGATGCTTATGTCTCAGTTATTCCCCGCTTGTGAATACACCCAAATAATTCCTCAACAAGGGGAGAAGAAGCCAAAGCCGAGACGCAAGCGTAGTAAGAAGAACAAAAGTGGAGAAAACAGTGCTAACCAAATATTGAACAAAAAGAGGAAATTGAGTGATGAACAGATGAAAATTTTGGAGGAAAATTTTGGAAATGAACATAAACTTGAGTCAGAGAAGAAGGATAGGCTTGCAATGGAGCTTGGTTTAGATCCCAGACAAGTTGCAGTATGGTTCCAGAATCGAAGAGCTAGGTGGAAGAACAAGAAACTTGAAGAAGAGTACTTAAGTCTTAAAAAAATTCATGAATCCACCATTCTTGAGAAGTATCTCCTTGAAACCAAGGTGTTGAAGCTAACGGAGCAACTCTCAGAATCTGAGAAAGAAAATCAAATGCTTCGAGAGCCAATTGAAAGGGTAACATGCAGCAGTTCAAGTACTTCATCCATGTCTCAGTCAGTGGAAGTTGTGGACCCACCTTTTCTTGGTGAATTTGGTGTTGATAACGGATATGACGATGATGTGTTTTTCATGCCTAATGAAACATGTTACTACAATGGCATGGAATGGATAAGTTTGTACATGTAG

Protein sequence

>MsaT025623.1

MNHQDQMMLMSQLFPACEYTQIIPQQGEKKPKPRRKRSKKNKSGENSANQILNKKRKLSDEQMKILEENFGNEHKLESEKKDRLAMELGLDPRQVAVWFQNRRARWKNKKLEEEYLSLKKIHESTILEKYLLETKVLKLTEQLSESEKENQMLREPIERVTCSSSSTSSMSQSVEVVDPPFLGEFGVDNGYDDDVFFMPNETCYYNGMEWISLYM*