AlfalfaGEDB | MsaG025628

M. sativa ssp. caerulea / MsaG025628

KEGG: K09338
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0007275 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009889 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022622 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0048364 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00046.32

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025628	XP_003611950.1	90.865	208	14	2	1	204	1	207	4.76e-127	367

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025628	sp\|Q9ZU70\|ATB21_ARATH	45.575	226	97	8	1	206	1	220	1.09e-55	179

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025628	tr\|G7KC76\|G7KC76_MEDTR	90.865	208	14	2	1	204	1	207	2.28e-127	367

TFs/TRs:

Gene ID	Type	Classification
MsaG025628	TF	HB-other

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025628	MtrunA17_Chr5g0403701	90.865	208	14	2	1	204	1	207	4.38e-131	367
MsaG025628	MtrunA17_Chr5g0403691	78.378	222	31	3	1	206	1	221	5.68e-118	334
MsaG025628	MtrunA17_Chr4g0060031	38.776	147	73	3	31	160	62	208	7.10e-23	94.0
MsaG025628	MtrunA17_Chr3g0123691	46.087	115	55	1	54	168	60	167	1.65e-22	92.4
MsaG025628	MtrunA17_Chr6g0450991	42.446	139	65	3	54	185	67	197	3.75e-22	91.3
MsaG025628	MtrunA17_Chr8g0381741	56.250	80	35	0	45	124	45	124	8.85e-22	90.5
MsaG025628	MtrunA17_Chr6g0453021	50.505	99	49	0	45	143	87	185	1.10e-20	87.0
MsaG025628	MtrunA17_Chr5g0415941	60.563	71	28	0	54	124	87	157	2.48e-20	87.0
MsaG025628	MtrunA17_Chr8g0362151	44.898	98	54	0	46	143	69	166	2.95e-20	86.3
MsaG025628	MtrunA17_Chr5g0416391	44.737	114	58	1	46	159	69	177	5.61e-20	85.5
MsaG025628	MtrunA17_Chr3g0127511	47.959	98	44	1	56	153	40	130	6.79e-20	84.3
MsaG025628	MtrunA17_Chr8g0362751	58.571	70	29	0	54	123	89	158	9.66e-20	84.7
MsaG025628	MtrunA17_Chr7g0265521	45.918	98	53	0	48	145	57	154	4.65e-19	82.0
MsaG025628	MtrunA17_Chr1g0179581	51.250	80	39	0	54	133	28	107	5.29e-19	78.6
MsaG025628	MtrunA17_Chr3g0119291	44.660	103	57	0	51	153	56	158	9.95e-19	81.6
MsaG025628	MtrunA17_Chr8g0346251	63.793	58	21	0	58	115	45	102	2.40e-17	77.8
MsaG025628	MtrunA17_Chr7g0217111	47.778	90	47	0	54	143	80	169	8.89e-16	73.9
MsaG025628	MtrunA17_Chr4g0044091	43.564	101	43	1	54	154	65	151	9.33e-14	67.0
MsaG025628	MtrunA17_Chr4g0055851	43.564	101	43	1	54	154	120	206	1.82e-13	67.4
MsaG025628	MtrunA17_Chr8g0335131	45.263	95	47	1	54	143	146	240	5.71e-13	66.2
MsaG025628	MtrunA17_Chr1g0175651	38.938	113	55	1	42	154	142	240	1.04e-12	65.5
MsaG025628	MtrunA17_Chr2g0308611	38.017	121	61	1	34	154	180	286	1.10e-12	65.9
MsaG025628	MtrunA17_Chr4g0054421	45.263	95	47	1	54	143	127	221	1.15e-12	65.1
MsaG025628	MtrunA17_Chr1g0151971	42.574	101	44	1	54	154	130	216	1.34e-12	65.1
MsaG025628	MtrunA17_Chr7g0258831	40.566	106	49	1	49	154	155	246	7.39e-12	63.2
MsaG025628	MtrunA17_Chr5g0400781	45.263	95	47	1	54	143	123	217	1.27e-11	62.0
MsaG025628	MtrunA17_Chr3g0135331	43.836	73	41	0	52	124	143	215	4.55e-11	60.8
MsaG025628	MtrunA17_Chr5g0399491	43.158	95	49	1	54	143	136	230	5.00e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025628	AT4G36740.2	50.237	211	91	7	7	206	10	217	1.03e-56	179
MsaG025628	AT2G18550.1	45.575	226	97	8	1	206	1	220	1.11e-56	179
MsaG025628	AT4G36740.1	50.711	211	89	8	7	206	10	216	4.27e-56	177
MsaG025628	AT5G66700.1	47.727	220	97	5	5	206	9	228	9.06e-47	154
MsaG025628	AT3G01470.1	33.333	207	104	5	2	174	13	219	2.37e-24	97.1
MsaG025628	AT1G69780.1	38.961	154	80	3	2	143	23	174	1.89e-23	95.1
MsaG025628	AT4G40060.1	63.380	71	26	0	54	124	59	129	7.52e-22	90.9
MsaG025628	AT2G22430.1	64.789	71	25	0	54	124	62	132	7.62e-22	91.3
MsaG025628	AT5G65310.2	57.143	77	33	0	43	119	43	119	2.93e-21	89.4
MsaG025628	AT5G65310.1	57.143	77	33	0	43	119	61	137	3.24e-21	89.7
MsaG025628	AT5G53980.1	37.313	134	84	0	45	178	2	135	2.29e-20	84.0
MsaG025628	AT2G46680.2	48.000	100	51	1	48	146	26	125	5.07e-20	85.5
MsaG025628	AT5G03790.1	39.683	126	75	1	14	139	38	162	5.35e-20	84.7
MsaG025628	AT2G46680.1	45.370	108	52	1	48	155	26	126	8.35e-20	84.7
MsaG025628	AT5G03790.2	40.496	121	71	1	19	139	43	162	9.53e-20	83.2
MsaG025628	AT1G26960.1	41.176	136	71	2	54	180	71	206	3.18e-19	83.2
MsaG025628	AT2G36610.1	45.370	108	51	2	46	145	63	170	5.44e-19	81.3
MsaG025628	AT3G61890.1	44.444	99	55	0	57	155	33	131	3.85e-18	80.1
MsaG025628	AT3G01220.1	51.852	81	38	1	45	124	77	157	4.65e-18	80.5
MsaG025628	AT5G15150.1	58.209	67	28	0	54	120	115	181	8.21e-18	80.1
MsaG025628	AT1G27045.1	42.149	121	57	1	52	159	66	186	3.20e-17	77.4
MsaG025628	AT1G27045.2	42.149	121	57	1	52	159	15	135	3.21e-17	76.3
MsaG025628	AT1G27045.5	42.149	121	57	1	52	159	23	143	3.27e-17	76.3
MsaG025628	AT1G27045.3	42.149	121	57	1	52	159	23	143	3.27e-17	76.3
MsaG025628	AT1G27045.4	55.882	68	30	0	52	119	66	133	2.88e-16	75.1
MsaG025628	AT2G22800.1	45.161	93	46	1	54	141	113	205	1.17e-12	65.5
MsaG025628	AT2G01430.1	36.975	119	57	2	54	168	139	243	2.43e-12	64.7
MsaG025628	AT4G16780.1	41.905	105	51	2	44	143	124	223	2.74e-12	64.7
MsaG025628	AT5G47370.1	35.659	129	78	1	20	143	96	224	8.25e-12	63.2
MsaG025628	AT4G37790.1	44.086	93	47	1	54	141	126	218	1.52e-11	62.4
MsaG025628	AT4G17460.1	40.385	104	57	1	45	143	126	229	1.58e-11	62.4
MsaG025628	AT5G06710.1	36.667	120	62	1	35	154	171	276	3.26e-11	62.0

Find 37 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCTTGTTGAGAAATTATT+TGG	0.079303	5:-10784345	None:intergenic
TCCACCTAGCCCTTCGATTC+TGG	0.220585	5:-10784670	None:intergenic
TGATGAACAAATGAAAATTT+TGG	0.274227	5:+10784550	MsaT025628.1:CDS
GAACCAAACTGCAACTTGTC+TGG	0.307826	5:-10784648	None:intergenic
GAAGTACTAGAGCTACTATT+TGG	0.364392	5:-10785757	None:intergenic
ATCCCAGACAAGTTGCAGTT+TGG	0.404850	5:+10784645	MsaT025628.1:CDS
TTCTTACTACGCTTGCGTCT+TGG	0.426044	5:-10784470	None:intergenic
TATAAAGATTAATCCATTCC+TGG	0.431532	5:-10785885	None:intergenic
ACCTTGTTGAGAAATTATTT+GGG	0.435398	5:-10784344	None:intergenic
AGAGGTATACCTTGGTTTCA+AGG	0.440743	5:-10784766	None:intergenic
ATTTGGGTGTATTCACAAGC+TGG	0.458844	5:-10784328	None:intergenic
ACTGAGACATAAGCATCATT+TGG	0.463969	5:-10784300	None:intergenic
TCAAATTCATCAAGTAATTG+TGG	0.464011	5:-10785799	None:intergenic
GATAGACTTGCAATGGAGCT+TGG	0.475779	5:+10784617	MsaT025628.1:CDS
AAGGAGACACTTCTCAAGAA+TGG	0.492858	5:-10784747	None:intergenic
CAAATTCATCAAGTAATTGT+GGG	0.494706	5:-10785798	None:intergenic
TAAACTTGAGTCAGAGAAGA+AGG	0.499532	5:+10784595	MsaT025628.1:CDS
AACCAAACTGCAACTTGTCT+GGG	0.501567	5:-10784647	None:intergenic
CAGTTTGGTTCCAGAATCGA+AGG	0.504708	5:+10784660	MsaT025628.1:CDS
TTTGGGTGTATTCACAAGCT+GGG	0.519334	5:-10784327	None:intergenic
GGTTCCAGAATCGAAGGGCT+AGG	0.526167	5:+10784666	MsaT025628.1:CDS
ATTACTTCAACGGCCAGGAA+TGG	0.529342	5:+10785872	MsaT025628.1:CDS
TTGAAGTAATATGTCGCATC+CGG	0.533058	5:-10785859	None:intergenic
GCAGCTTTCTGAGGCAGAGA+AGG	0.540038	5:+10785714	MsaT025628.1:CDS
GAAGAAGGATAGACTTGCAA+TGG	0.545678	5:+10784610	MsaT025628.1:CDS
TACTTCATCAATTTCAGTAG+TGG	0.546036	5:+10785774	MsaT025628.1:CDS
AGCTACTATTTGGCTCTCGA+AGG	0.571565	5:-10785747	None:intergenic
GATGCGACATATTACTTCAA+CGG	0.573154	5:+10785862	MsaT025628.1:CDS
GCTAACAGAGCAGCTTTCTG+AGG	0.593252	5:+10785705	MsaT025628.1:CDS
CGTAGTAAGAAGAACAAAGG+TGG	0.606299	5:+10784482	MsaT025628.1:CDS
GAGACACTTCTCAAGAATGG+TGG	0.632257	5:-10784744	None:intergenic
GAAGTGTCTCCTTGAAACCA+AGG	0.638232	5:+10784757	MsaT025628.1:CDS
GACATATTACTTCAACGGCC+AGG	0.639581	5:+10785867	MsaT025628.1:CDS
ACCCAAATAATTTCTCAACA+AGG	0.645027	5:+10784343	MsaT025628.1:CDS
TCCAGAATCGAAGGGCTAGG+TGG	0.655135	5:+10784669	MsaT025628.1:CDS
AAGCGTAGTAAGAAGAACAA+AGG	0.668285	5:+10784479	MsaT025628.1:CDS
AGTTTGGTTCCAGAATCGAA+GGG	0.690392	5:+10784661	MsaT025628.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	10784286	10785912	10784286	ID=MsaG025628
Chr5	mRNA	10784286	10785912	10784286	ID=MsaT025628.1;Parent=MsaG025628
Chr5	exon	10784286	10784364	10784286	ID=MsaT025628.1.exon1;Parent=MsaT025628.1
Chr5	CDS	10784286	10784364	10784286	ID=cds.MsaT025628.1;Parent=MsaT025628.1
Chr5	exon	10784453	10784778	10784453	ID=MsaT025628.1.exon2;Parent=MsaT025628.1
Chr5	CDS	10784453	10784778	10784453	ID=cds.MsaT025628.1;Parent=MsaT025628.1
Chr5	exon	10785697	10785912	10785697	ID=MsaT025628.1.exon3;Parent=MsaT025628.1
Chr5	CDS	10785697	10785912	10785697	ID=cds.MsaT025628.1;Parent=MsaT025628.1

Gene Sequence

>MsaT025628.1

ATGAATCATCAAGACCAAATGATGCTTATGTCTCAGTTATTCCCAGCTTGTGAATACACCCAAATAATTTCTCAACAAGGGGAGAAGAAGCTAAAGCCAAGACGCAAGCGTAGTAAGAAGAACAAAGGTGGAGAAAACAGCGCTAACCAAATATTGAACAAAAAGAGGAAATTGAGTGATGAACAAATGAAAATTTTGGAAGAAAATTTTGGAAATGAACATAAACTTGAGTCAGAGAAGAAGGATAGACTTGCAATGGAGCTTGGTTTAGATCCCAGACAAGTTGCAGTTTGGTTCCAGAATCGAAGGGCTAGGTGGAAGAACAAGAAACTTGAAGAAGAGTACTTAACTCTTAAAAAAATTCATGAATCCACCATTCTTGAGAAGTGTCTCCTTGAAACCAAGATGTTGAAGCTAACAGAGCAGCTTTCTGAGGCAGAGAAGGAAATTCAACGCCTTCGAGAGCCAAATAGTAGCTCTAGTACTTCATCAATTTCAGTAGTGGACCCACAATTACTTGATGAATTTGAAGTTAATCATATATATAATGATGATGTGTTTTTCATGCCGGATGCGACATATTACTTCAACGGCCAGGAATGGATTAATCTTTATATCTAA

Protein sequence

>MsaT025628.1

MNHQDQMMLMSQLFPACEYTQIISQQGEKKLKPRRKRSKKNKGGENSANQILNKKRKLSDEQMKILEENFGNEHKLESEKKDRLAMELGLDPRQVAVWFQNRRARWKNKKLEEEYLTLKKIHESTILEKCLLETKMLKLTEQLSEAEKEIQRLREPNSSSSTSSISVVDPQLLDEFEVNHIYNDDVFFMPDATYYFNGQEWINLYI*