AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG025726


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025726 MtrunA17_Chr5g0405151 100.000 184 0 0 1 184 1 184 3.61e-138 385
MsaG025726 MtrunA17_Chr2g0314671 50.000 104 49 2 1 101 1 104 7.85e-26 102
MsaG025726 MtrunA17_Chr4g0033641 42.537 134 71 4 1 131 1 131 3.15e-24 97.1
MsaG025726 MtrunA17_Chr3g0145061 43.000 100 53 2 1 96 1 100 2.39e-23 94.4
MsaG025726 MtrunA17_Chr2g0329511 47.959 98 48 2 1 95 1 98 6.17e-23 93.6
MsaG025726 MtrunA17_Chr3g0142221 42.105 114 61 2 1 109 1 114 6.17e-23 93.2
MsaG025726 MtrunA17_Chr4g0035651 44.000 100 52 2 1 96 1 100 3.63e-22 91.3
MsaG025726 MtrunA17_Chr1g0155811 46.000 100 50 2 1 96 1 100 1.04e-20 88.6
MsaG025726 MtrunA17_Chr3g0136701 50.602 83 33 1 4 86 5 79 4.51e-20 87.0
MsaG025726 MtrunA17_Chr1g0150471 44.211 95 43 2 4 96 5 91 3.36e-18 82.0
MsaG025726 MtrunA17_Chr7g0220721 48.750 80 33 1 5 84 26 97 1.99e-17 80.9
MsaG025726 MtrunA17_Chr4g0072871 43.210 81 38 1 4 84 21 93 7.42e-16 76.3
MsaG025726 MtrunA17_Chr7g0268691 38.750 80 41 1 5 84 20 91 7.70e-13 67.4
MsaG025726 MtrunA17_Chr3g0120711 33.036 112 63 2 1 112 1 100 4.31e-11 62.4
MsaG025726 MtrunA17_Chr5g0429341 36.905 84 45 1 1 84 1 76 5.54e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025726 AT4G38960.1 60.870 184 44 3 1 184 1 156 5.77e-74 223
MsaG025726 AT4G38960.6 60.870 184 44 3 1 184 1 156 5.77e-74 223
MsaG025726 AT2G21320.1 60.326 184 46 3 1 184 1 157 3.88e-70 213
MsaG025726 AT4G38960.3 49.558 226 43 4 1 183 1 198 9.94e-66 204
MsaG025726 AT4G38960.4 55.263 152 40 3 34 185 1 124 1.43e-51 165
MsaG025726 AT4G38960.5 55.333 150 39 3 34 183 1 122 1.00e-50 163
MsaG025726 AT4G38960.2 55.333 150 39 3 34 183 1 122 1.00e-50 163
MsaG025726 AT1G78600.1 50.000 104 49 2 1 101 1 104 8.16e-27 105
MsaG025726 AT1G78600.2 50.000 104 49 2 1 101 1 104 9.97e-27 105
MsaG025726 AT1G06040.2 50.000 98 46 2 1 95 1 98 1.12e-23 94.4
MsaG025726 AT4G39070.1 46.000 100 50 2 1 96 1 100 3.02e-23 95.1
MsaG025726 AT1G75540.1 45.098 102 50 2 1 96 1 102 3.37e-23 96.7
MsaG025726 AT1G06040.1 50.000 98 46 2 1 95 1 98 3.96e-23 94.7
MsaG025726 AT2G24790.2 36.994 173 87 9 4 166 7 167 1.56e-20 87.0
MsaG025726 AT2G31380.1 38.732 142 69 3 1 124 1 142 2.41e-20 87.0
MsaG025726 AT2G24790.1 47.826 92 38 2 4 93 7 90 7.73e-20 86.7
MsaG025726 AT5G24930.1 48.148 81 34 1 4 84 5 77 9.16e-19 84.7
MsaG025726 AT5G15850.1 47.253 91 38 2 5 93 12 94 1.35e-18 84.0
MsaG025726 AT4G10240.1 42.718 103 50 2 1 96 1 101 1.56e-18 80.5
MsaG025726 AT3G02380.1 48.750 80 33 1 5 84 16 87 9.23e-18 81.6
MsaG025726 AT5G15840.1 50.000 80 32 1 5 84 20 91 2.19e-17 80.9
MsaG025726 AT2G47890.3 42.222 90 44 1 4 93 12 93 2.59e-17 80.1
MsaG025726 AT2G47890.1 42.222 90 44 1 4 93 12 93 2.67e-17 80.1
MsaG025726 AT5G15840.2 50.000 80 32 1 5 84 20 91 3.30e-17 79.3
MsaG025726 AT2G47890.2 42.222 90 44 1 4 93 12 93 6.01e-17 77.8
MsaG025726 AT5G57660.1 42.500 80 34 2 5 84 22 89 4.29e-13 68.6
MsaG025726 AT1G68190.1 38.710 93 49 1 1 93 10 94 1.03e-12 67.4
MsaG025726 AT1G68190.3 38.710 93 49 1 1 93 10 94 1.03e-12 67.4
MsaG025726 AT1G28050.1 42.169 83 40 1 2 84 6 80 1.22e-12 67.4
MsaG025726 AT1G68190.2 38.710 93 49 1 1 93 39 123 1.23e-12 67.0
MsaG025726 AT2G33500.2 40.000 80 40 1 5 84 12 83 4.18e-11 62.8
MsaG025726 AT2G33500.1 40.000 80 40 1 5 84 12 83 4.22e-11 62.8

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCATGTATTCTGTTGTT+AGG 0.314824 5:+11947715 None:intergenic
CTCTTTGTTTCATAGTTTCC+AGG 0.319859 5:-11947937 MsaT025726.1:intron
AATAATCAGGTTAGAGTTCA+TGG 0.327088 5:-11947685 MsaT025726.1:CDS
AGTTAGCTAGTAGACATGTT+AGG 0.334611 5:-11948533 MsaT025726.1:CDS
TTGCTTTGTAGCACGATCTA+TGG 0.342553 5:+11946826 None:intergenic
ATGGAAGATATCTTCTCTTT+AGG 0.344786 5:-11948282 MsaT025726.1:CDS
TTAGAATTCCCATCAGCATT+AGG 0.367971 5:+11947772 None:intergenic
TGAACATGCATCAAATAATC+AGG 0.417562 5:-11947698 MsaT025726.1:CDS
GCCGGGTTGTCTGCATTACT+AGG 0.455216 5:+11947907 None:intergenic
GAGAGAAGCAACAAAATCAT+AGG 0.461871 5:-11947819 MsaT025726.1:CDS
GGATCTAGAGGTTGTGAAGC+CGG 0.462473 5:+11947889 None:intergenic
AGTGAAATTGATAAACGTAT+TGG 0.464815 5:-11947575 MsaT025726.1:CDS
AACGTATTGGCTGTTGCTAC+TGG 0.479764 5:-11947562 MsaT025726.1:intron
TCAGCATTAGGTTCTGAAGT+TGG 0.480629 5:+11947784 None:intergenic
CAGCCATAGGTTGTGCTACA+AGG 0.481804 5:-11946886 MsaT025726.1:CDS
TCGCACCGCGGCACGTCACT+CGG 0.487301 5:+11948495 None:intergenic
CATATCACATTGCAAACAAA+GGG 0.494623 5:+11948333 None:intergenic
CTGCCTTGTAGCACAACCTA+TGG 0.498589 5:+11946883 None:intergenic
GTTAGCTAGTAGACATGTTA+GGG 0.499857 5:-11948532 MsaT025726.1:CDS
TTAGGTTCTGAAGTTGGAAC+CGG 0.500416 5:+11947790 None:intergenic
AAGAATTTGTTCTCTCATGT+AGG 0.509169 5:-11946793 MsaT025726.1:CDS
GCATTACTAGGTTTATCGCC+TGG 0.511209 5:+11947919 None:intergenic
TGCTGATGGGAATTCTAAGA+TGG 0.518367 5:-11947767 MsaT025726.1:CDS
GATCCAGGTGATATTAAAAG+AGG 0.532561 5:-11947871 MsaT025726.1:CDS
GATCTAGAGGTTGTGAAGCC+GGG 0.534120 5:+11947890 None:intergenic
TCATATCACATTGCAAACAA+AGG 0.534969 5:+11948332 None:intergenic
TTAATATCACCTGGATCTAG+AGG 0.539036 5:+11947877 None:intergenic
GCTTCACAACCTCTAGATCC+AGG 0.543997 5:-11947886 MsaT025726.1:CDS
TCTCTTTAGGCAAAGAGTTG+AGG 0.545750 5:-11948269 MsaT025726.1:intron
TTCTTCTATTGCGAAACAGA+TGG 0.548785 5:-11948361 MsaT025726.1:CDS
ACTTCAGAACCTAATGCTGA+TGG 0.548946 5:-11947781 MsaT025726.1:CDS
GCTAGTAGACATGTTAGGGT+TGG 0.553453 5:-11948528 MsaT025726.1:CDS
ACCTAGTAATGCAGACAACC+CGG 0.558690 5:-11947908 MsaT025726.1:CDS
CAAGGCAGTCTGATTCCGAC+AGG 0.563913 5:-11946868 MsaT025726.1:CDS
ACAAAATCATAGGATGCCTC+CGG 0.578363 5:-11947809 MsaT025726.1:CDS
GTTGGTGGTAAAAGAACGCA+TGG 0.582566 5:-11948301 MsaT025726.1:CDS
ATAATCAGGTTAGAGTTCAT+GGG 0.583574 5:-11947684 MsaT025726.1:CDS
CAAGTCCGAGTGACGTGCCG+CGG 0.583612 5:-11948500 MsaT025726.1:CDS
CTTCAGAACCTAATGCTGAT+GGG 0.588602 5:-11947780 MsaT025726.1:CDS
CTGTCGTGCTTGTGATGAGA+AGG 0.598560 5:-11949054 MsaT025726.1:intron
GGTTCTGAAGTTGGAACCGG+AGG 0.621508 5:+11947793 None:intergenic
GACATATGTGAGAATGCCCC+TGG 0.645262 5:-11948474 MsaT025726.1:intron
GATATGATAGTTCATGTTGG+TGG 0.666400 5:-11948316 MsaT025726.1:CDS
TGTGATATGATAGTTCATGT+TGG 0.669655 5:-11948319 MsaT025726.1:CDS
AGCAGCACAGAAAACTATAG+CGG 0.689026 5:+11949091 None:intergenic
TTTCTGATGTAAGATCCTGT+CGG 0.715499 5:+11946853 None:intergenic
TTCTCACATATGTCGCACCG+CGG 0.743597 5:+11948483 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 11946778 11949147 11946778 ID=MsaG025726
Chr5 mRNA 11946778 11949147 11946778 ID=MsaT025726.1;Parent=MsaG025726
Chr5 exon 11946778 11946905 11946778 ID=MsaT025726.1.exon6;Parent=MsaT025726.1
Chr5 CDS 11946778 11946905 11946778 ID=cds.MsaT025726.1;Parent=MsaT025726.1
Chr5 exon 11947563 11947618 11947563 ID=MsaT025726.1.exon5;Parent=MsaT025726.1
Chr5 CDS 11947563 11947618 11947563 ID=cds.MsaT025726.1;Parent=MsaT025726.1
Chr5 exon 11947682 11947944 11947682 ID=MsaT025726.1.exon4;Parent=MsaT025726.1
Chr5 CDS 11947682 11947944 11947682 ID=cds.MsaT025726.1;Parent=MsaT025726.1
Chr5 exon 11948270 11948385 11948270 ID=MsaT025726.1.exon3;Parent=MsaT025726.1
Chr5 CDS 11948270 11948385 11948270 ID=cds.MsaT025726.1;Parent=MsaT025726.1
Chr5 exon 11948475 11948571 11948475 ID=MsaT025726.1.exon2;Parent=MsaT025726.1
Chr5 CDS 11948475 11948571 11948475 ID=cds.MsaT025726.1;Parent=MsaT025726.1
Chr5 exon 11949055 11949147 11949055 ID=MsaT025726.1.exon1;Parent=MsaT025726.1
Chr5 CDS 11949055 11949147 11949055 ID=cds.MsaT025726.1;Parent=MsaT025726.1
Gene Sequence

>MsaT025726.1

ATGCGAACGCTTTGTGATGCTTGTGAGAGCGCTGCCGCTATAGTTTTCTGTGCTGCTGATGAGGCTGCACTCTGTCGTGCTTGTGATGAGAAGGTTCATATGTGCAATAAGTTAGCTAGTAGACATGTTAGGGTTGGTCTTGCAAGTCCGAGTGACGTGCCGCGGTGCGACATATGTGAGAATGCCCCTGCTTTCTTCTATTGCGAAACAGATGGAAGTTCCCTTTGTTTGCAATGTGATATGATAGTTCATGTTGGTGGTAAAAGAACGCATGGAAGATATCTTCTCTTTAGGCAAAGAGTTGAGTTTCCAGGCGATAAACCTAGTAATGCAGACAACCCGGCTTCACAACCTCTAGATCCAGGTGATATTAAAAGAGGACAAAGTCCACTTCCTAAACAAAAAATGGGAGAGAAGCAACAAAATCATAGGATGCCTCCGGTTCCAACTTCAGAACCTAATGCTGATGGGAATTCTAAGATGGAAAATAAATTGATTGATTTGAACATGAAGCCTAACAACAGAATACATGAACATGCATCAAATAATCAGGTTAGAGTTCATGGGAAATACTGCAAAATATACTTAAGAGTGAAATTGATAAACGTATTGGCTGTTGCTACTGCCATAGGTTGTGCTACAAGGCAGTCTGATTCCGACAGGATCTTACATCAGAAATCTACCATAGATCGTGCTACAAAGCAATGCGAGTCTAAAGAATTTGTTCTCTCATGTAGGAATTTAGTTTTTTAG

Protein sequence

>MsaT025726.1

MRTLCDACESAAAIVFCAADEAALCRACDEKVHMCNKLASRHVRVGLASPSDVPRCDICENAPAFFYCETDGSSLCLQCDMIVHVGGKRTHGRYLLFRQRVEFPGDKPSNADNPASQPLDPGDIKRGQSPLPKQKMGEKQQNHRMPPVPTSEPNADGNSKMENKLIDLNMKPNNRIHEHASNNQVRVHGKYCKIYLRVKLINVLAVATAIGCATRQSDSDRILHQKSTIDRATKQCESKEFVLSCRNLVF*