AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG025750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025750 MtrunA17_Chr5g0405351 96.466 283 8 2 1 283 22 302 0.0 568
MsaG025750 MtrunA17_Chr4g0043891 67.164 67 21 1 5 71 4 69 6.98e-26 102
MsaG025750 MtrunA17_Chr8g0392991 70.769 65 18 1 7 71 94 157 1.08e-25 104
MsaG025750 MtrunA17_Chr5g0436661 59.459 74 26 2 9 79 33 105 1.50e-24 97.4
MsaG025750 MtrunA17_Chr7g0238431 67.692 65 20 1 7 71 6 69 5.09e-24 96.7
MsaG025750 MtrunA17_Chr4g0031341 68.254 63 19 1 9 71 25 86 9.55e-24 97.1
MsaG025750 MtrunA17_Chr1g0153171 64.062 64 22 1 8 71 89 151 1.57e-22 93.2
MsaG025750 MtrunA17_Chr8g0385441 78.723 47 10 0 25 71 97 143 1.84e-19 83.2
MsaG025750 MtrunA17_Chr3g0125111 76.596 47 11 0 25 71 95 141 3.27e-19 82.4
MsaG025750 MtrunA17_Chr7g0223471 48.438 64 32 1 9 72 47 109 4.87e-15 74.7
MsaG025750 MtrunA17_Chr2g0283141 44.928 69 37 1 3 71 49 116 3.37e-14 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG025750 AT2G17950.1 41.699 259 103 9 4 236 33 269 1.52e-47 160
MsaG025750 AT3G18010.1 70.769 65 18 1 7 71 74 137 1.00e-25 104
MsaG025750 AT5G59340.1 69.697 66 19 1 6 71 11 75 1.08e-25 102
MsaG025750 AT2G28610.1 65.152 66 22 1 6 71 5 69 2.64e-23 95.9
MsaG025750 AT3G11260.1 65.079 63 21 1 9 71 24 85 4.03e-23 94.0
MsaG025750 AT5G05770.1 62.319 69 25 1 9 77 29 96 5.22e-23 91.7
MsaG025750 AT1G46480.2 64.062 64 22 1 8 71 89 151 1.25e-22 92.8
MsaG025750 AT1G46480.1 64.062 64 22 1 8 71 89 151 7.87e-22 92.0
MsaG025750 AT2G01500.1 61.017 59 22 1 6 64 58 115 5.30e-19 84.7
MsaG025750 AT5G45980.1 52.381 63 29 1 9 71 55 116 5.36e-15 74.3
MsaG025750 AT2G33880.2 45.714 70 37 1 3 72 49 117 1.12e-14 73.9
MsaG025750 AT2G33880.1 45.714 70 37 1 3 72 49 117 1.18e-14 73.6
MsaG025750 AT1G20700.2 39.130 69 41 1 9 77 33 100 7.67e-11 59.7

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCCTAAGTGAGGGTTTA+TGG 0.166513 5:+12284642 None:intergenic
AATGGGAGGGTTTCAATTTC+AGG 0.198475 5:+12284505 None:intergenic
CTGTTATTGCTGCTGCTAAT+TGG 0.280712 5:-12285483 MsaT025750.1:CDS
CAAATTTAATTAGCATAATC+TGG 0.300562 5:+12284322 None:intergenic
TTTGCATAAGCTGAAGAATA+TGG 0.309877 5:+12284598 None:intergenic
TCTTCCATGATTTCTTCATT+TGG 0.323131 5:+12284556 None:intergenic
CTCTGAATCTGTTCTGCACT+TGG 0.338799 5:+12285658 None:intergenic
TATGAACAAGGACAAGAAAA+TGG 0.342235 5:-12284532 MsaT025750.1:CDS
ATTCTTATCTGATCTGTTGT+TGG 0.355808 5:+12285718 None:intergenic
CAGATTGGTAACTATGGATA+TGG 0.369373 5:-12285230 MsaT025750.1:CDS
TCAGCATGCATAGGGAATAA+TGG 0.370597 5:+12284487 None:intergenic
AAATCAAATTCATCTAACTA+TGG 0.374371 5:-12284436 MsaT025750.1:CDS
CAGCATGCATAGGGAATAAT+GGG 0.394880 5:+12284488 None:intergenic
GCAATAACAGTATTGTTGTT+TGG 0.399113 5:+12285496 None:intergenic
GTAGGCCAGATTGGTAACTA+TGG 0.413013 5:-12285236 MsaT025750.1:CDS
TCTGCTAGGTTAAGACAGTA+TGG 0.419676 5:-12285631 MsaT025750.1:CDS
TGTTGTTGTTCATGTTGATC+AGG 0.421893 5:+12285457 None:intergenic
GCCTCAACTCTTACGGTTGT+AGG 0.423475 5:-12284348 MsaT025750.1:CDS
AATTACAACATTTCATCTGC+TGG 0.424173 5:-12285403 MsaT025750.1:intron
TGGTTCTCGTACTACTTCCT+TGG 0.429314 5:-12284377 MsaT025750.1:CDS
ATGAAATGTTGTAATTAGAA+TGG 0.431892 5:+12285410 None:intergenic
CTAGAGAAAGACAGAAGAAA+AGG 0.441919 5:-12285561 MsaT025750.1:CDS
GTTTGGTGCTCTTTGAATGA+TGG 0.443486 5:+12285513 None:intergenic
CATATCCATAGTTACCAATC+TGG 0.452599 5:+12285231 None:intergenic
TTTGGTGCTCTTTGAATGAT+GGG 0.453249 5:+12285514 None:intergenic
GAACTTAGCCTCAACTCTTA+CGG 0.457853 5:-12284355 MsaT025750.1:CDS
AAGACCAAATGAAGAAATCA+TGG 0.463541 5:-12284560 MsaT025750.1:CDS
ATGGTTACTGTAGGCCAGAT+TGG 0.471455 5:-12285245 MsaT025750.1:CDS
AGTTAGATGAATTTGATTTG+AGG 0.476215 5:+12284440 None:intergenic
AGATCAGATAAGAATATTGA+AGG 0.488670 5:-12285710 MsaT025750.1:CDS
TTCATGTTCTTCTGCTGAGA+TGG 0.491391 5:-12285264 MsaT025750.1:CDS
AACCCTCACTTAGGATGGAT+TGG 0.497436 5:-12284637 MsaT025750.1:CDS
AAGGATCTTTACTACAACAA+TGG 0.504008 5:-12285691 MsaT025750.1:CDS
AGACAGTATGGTAAGATTGA+AGG 0.507784 5:-12285619 MsaT025750.1:CDS
GGATGTAGCAGTAGTCAAGT+TGG 0.507987 5:-12284670 MsaT025750.1:CDS
TAGGAGTGCACAATATCAGC+TGG 0.529304 5:-12284691 MsaT025750.1:CDS
ATGCATGCTGAAGACATTCA+TGG 0.531289 5:-12284475 MsaT025750.1:CDS
TGGTATCAAGCTGAAGATGC+AGG 0.538672 5:-12284409 MsaT025750.1:CDS
CCATAAACCCTCACTTAGGA+TGG 0.538741 5:-12284642 MsaT025750.1:CDS
GAAGATGCAGGATTCATGTA+TGG 0.540471 5:-12284397 MsaT025750.1:CDS
GAATGTCTTCAGCATGCATA+GGG 0.542541 5:+12284479 None:intergenic
AATATGGATCAACATGATGA+TGG 0.557603 5:+12284614 None:intergenic
AGAGTTGAGGCTAAGTTCCA+AGG 0.568194 5:+12284360 None:intergenic
TGAATGTCTTCAGCATGCAT+AGG 0.569037 5:+12284478 None:intergenic
TCTGCTGAGATGGTTACTGT+AGG 0.577564 5:-12285254 MsaT025750.1:CDS
ATTCATCTAACTATGGTGGT+TGG 0.584464 5:-12284429 MsaT025750.1:CDS
TCAAATTCATCTAACTATGG+TGG 0.598577 5:-12284433 MsaT025750.1:CDS
AGTACCATAAACCCTCACTT+AGG 0.599826 5:-12284646 MsaT025750.1:CDS
TGGATATGGATCTGTCCCAA+TGG 0.601538 5:-12285216 MsaT025750.1:CDS
AGATTCAGAGAATCTCTGCT+AGG 0.608727 5:-12285645 MsaT025750.1:CDS
ACCTACAACCGTAAGAGTTG+AGG 0.617544 5:+12284347 None:intergenic
GGCCAATCCATCCTAAGTGA+GGG 0.618647 5:+12284635 None:intergenic
GCAGCAGACAAAGTAGTACA+AGG 0.645018 5:-12285747 MsaT025750.1:CDS
TGGCCAATCCATCCTAAGTG+AGG 0.646941 5:+12284634 None:intergenic
CATGCATAGGGAATAATGGG+AGG 0.647449 5:+12284491 None:intergenic
ATGCATAGGGAATAATGGGA+GGG 0.659824 5:+12284492 None:intergenic
ATCATGGAAGAATATGAACA+AGG 0.690834 5:-12284544 MsaT025750.1:CDS
GCAGACAAAGTAGTACAAGG+TGG 0.749876 5:-12285744 MsaT025750.1:CDS
CATGCTGAAGACATTCATGG+TGG 0.790217 5:-12284472 MsaT025750.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 12284327 12285773 12284327 ID=MsaG025750
Chr5 mRNA 12284327 12285773 12284327 ID=MsaT025750.1;Parent=MsaG025750
Chr5 exon 12284327 12284710 12284327 ID=MsaT025750.1.exon3;Parent=MsaT025750.1
Chr5 CDS 12284327 12284710 12284327 ID=cds.MsaT025750.1;Parent=MsaT025750.1
Chr5 exon 12285202 12285299 12285202 ID=MsaT025750.1.exon2;Parent=MsaT025750.1
Chr5 CDS 12285202 12285299 12285202 ID=cds.MsaT025750.1;Parent=MsaT025750.1
Chr5 exon 12285404 12285773 12285404 ID=MsaT025750.1.exon1;Parent=MsaT025750.1
Chr5 CDS 12285404 12285773 12285404 ID=cds.MsaT025750.1;Parent=MsaT025750.1
Gene Sequence

>MsaT025750.1

ATGGGCAGCAGACAAAGTAGTACAAGGTGGACACCAACAACAGATCAGATAAGAATATTGAAGGATCTTTACTACAACAATGGAATTAGATCACCAAGTGCAGAACAGATTCAGAGAATCTCTGCTAGGTTAAGACAGTATGGTAAGATTGAAGGCAAAAATGTCTTTTATTGGTTTCAAAATCACAAAGCTAGAGAAAGACAGAAGAAAAGGTTCACTTCTGATGTTAATGTTGTTCCCATCATTCAAAGAGCACCAAACAACAATACTGTTATTGCTGCTGCTAATTGGAAACCTGATCAACATGAACAACAACAACAAAACATTAATCTTCACACAAACCATTCTAATTACAACATTTCATCTGCTGGGCTTTCTTCTGCTTCATGTTCTTCTGCTGAGATGGTTACTGTAGGCCAGATTGGTAACTATGGATATGGATCTGTCCCAATGGAAAAGAGTTTTAGGGAGTGCACAATATCAGCTGGATGTAGCAGTAGTCAAGTTGGAAGTACCATAAACCCTCACTTAGGATGGATTGGCCATCATCATGTTGATCCATATTCTTCAGCTTATGCAAACTTATTTGAAAAAATAAGACCAAATGAAGAAATCATGGAAGAATATGAACAAGGACAAGAAAATGGTTCACCTGAAATTGAAACCCTCCCATTATTCCCTATGCATGCTGAAGACATTCATGGTGGCTATTGTAACCTCAAATCAAATTCATCTAACTATGGTGGTTGGTATCAAGCTGAAGATGCAGGATTCATGTATGGTTCTCGTACTACTTCCTTGGAACTTAGCCTCAACTCTTACGGTTGTAGGTCACCAGATTATGCTAATTAA

Protein sequence

>MsaT025750.1

MGSRQSSTRWTPTTDQIRILKDLYYNNGIRSPSAEQIQRISARLRQYGKIEGKNVFYWFQNHKARERQKKRFTSDVNVVPIIQRAPNNNTVIAAANWKPDQHEQQQQNINLHTNHSNYNISSAGLSSASCSSAEMVTVGQIGNYGYGSVPMEKSFRECTISAGCSSSQVGSTINPHLGWIGHHHVDPYSSAYANLFEKIRPNEEIMEEYEQGQENGSPEIETLPLFPMHAEDIHGGYCNLKSNSSNYGGWYQAEDAGFMYGSRTTSLELSLNSYGCRSPDYAN*