AlfalfaGEDB | MsaG025853

M. sativa ssp. caerulea / MsaG025853

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025853	AES95288.1	59.218	179	32	4	6	158	4	167	3.21e-56	184

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025853	tr\|G7K021\|G7K021_MEDTR	59.218	179	32	4	6	158	4	167	1.53e-56	184

TFs/TRs:

Gene ID	Type	Classification
MsaG025853	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000013	MsaG025853	0.806378	1.435147e-49	2.247253e-47
MsaG003754	MsaG025853	0.861799	1.572994e-63	1.309791e-60
MsaG003961	MsaG025853	0.823470	2.405822e-53	5.804820e-51
MsaG004525	MsaG025853	0.816671	8.517733e-52	1.717938e-49
MsaG005020	MsaG025853	0.832697	1.476170e-55	4.618583e-53
MsaG006279	MsaG025853	0.860244	4.648106e-63	3.648204e-60
MsaG006346	MsaG025853	0.828551	1.511126e-54	4.196923e-52
MsaG007445	MsaG025853	0.837450	9.465256e-57	3.413596e-54
MsaG008253	MsaG025853	0.824792	1.181074e-53	2.954066e-51
MsaG009109	MsaG025853	0.809622	2.950538e-50	4.992912e-48
MsaG010531	MsaG025853	0.818811	2.817134e-52	6.004824e-50
MsaG011561	MsaG025853	0.838896	4.032204e-57	1.520712e-54
MsaG012429	MsaG025853	0.816510	9.251583e-52	1.858288e-49
MsaG045313	MsaG025853	0.897757	2.347704e-76	1.055412e-72
MsaG045514	MsaG025853	0.871142	1.747367e-66	2.125142e-63
MsaG046573	MsaG025853	0.849085	7.692025e-60	4.041038e-57
MsaG046767	MsaG025853	0.818413	3.463414e-52	7.306395e-50
MsaG046825	MsaG025853	0.804677	3.251835e-49	4.893071e-47
MsaG025853	MsaG038129	0.801442	1.506037e-48	2.103703e-46
MsaG025853	MsaG035247	0.868040	1.770497e-65	1.891784e-62
MsaG016670	MsaG025853	0.826072	5.897377e-54	1.528184e-51
MsaG017883	MsaG025853	0.805838	1.862463e-49	2.879280e-47
MsaG017976	MsaG025853	0.827900	2.165692e-54	5.905793e-52
MsaG019271	MsaG025853	0.803232	6.471713e-49	9.417809e-47
MsaG020569	MsaG025853	0.878642	4.997589e-69	8.471235e-66
MsaG021424	MsaG025853	0.813542	4.184897e-51	7.798851e-49
MsaG021576	MsaG025853	0.807619	7.865205e-50	1.268261e-47
MsaG025713	MsaG025853	0.805318	2.391454e-49	3.652471e-47
MsaG025735	MsaG025853	0.811345	1.257492e-50	2.219170e-48
MsaG025778	MsaG025853	0.804140	4.201589e-49	6.244163e-47
MsaG028160	MsaG025853	0.908558	3.598493e-81	3.099397e-77
MsaG029363	MsaG025853	0.802124	1.092609e-48	1.550017e-46
MsaG029548	MsaG025853	0.815264	1.748898e-51	3.403523e-49
MsaG029619	MsaG025853	0.830146	6.223539e-55	1.808667e-52
MsaG029761	MsaG025853	0.860902	2.943401e-63	2.368297e-60
MsaG032487	MsaG025853	0.823176	2.815638e-53	6.739791e-51
MsaG033853	MsaG025853	0.818926	2.653440e-52	5.673126e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025853	MtrunA17_Chr5g0406781	70.543	129	21	3	6	130	4	119	5.75e-56	172

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 72 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTAGTTGGGTTGGTTTCTT+TGG	0.162115	5:-13502123	None:intergenic
TTCTTGGAAGGGTTAGGTTT+AGG	0.193850	5:-13502044	None:intergenic
TCTTGGAAGGGTTAGGTTTA+GGG	0.252279	5:-13502043	None:intergenic
GTAGTTGGGTTGGTTTCTTT+GGG	0.266039	5:-13502122	None:intergenic
AGGGGACTTGTTGGTGGTGG+GGG	0.282219	5:-13502072	None:intergenic
TTGAGGGGACTTGTTGGTGG+TGG	0.297192	5:-13502075	None:intergenic
GGCCTTCCTTCCTCTGGTCC+AGG	0.299430	5:-13502938	None:intergenic
GGGCTTCTTGATCGTCCTCT+TGG	0.324765	5:-13502960	None:intergenic
TGCTTATCAGTTGTATCGGC+TGG	0.335441	5:-13502981	None:intergenic
TAGTTGGGTTGGTTTCTTTG+GGG	0.344689	5:-13502121	None:intergenic
TGAGGGGACTTGTTGGTGGT+GGG	0.348447	5:-13502074	None:intergenic
GTGGAATTCTTGGAAGGGTT+AGG	0.374308	5:-13502050	None:intergenic
GGCTTCTTGATCGTCCTCTT+GGG	0.380085	5:-13502959	None:intergenic
GAGGGGACTTGTTGGTGGTG+GGG	0.390260	5:-13502073	None:intergenic
AAAGAACTCTGAAGGGTAGT+TGG	0.402173	5:-13502137	None:intergenic
TCAGAGTCCTCTATCTCTAT+TGG	0.410192	5:-13502866	None:intergenic
CTCTTGGGCCTTCCTTCCTC+TGG	0.410975	5:-13502944	None:intergenic
CAGGGTTACTGTTTGCACCT+TGG	0.424058	5:-13502919	None:intergenic
AGGGTCTTGAGGGGACTTGT+TGG	0.426514	5:-13502081	None:intergenic
AAGAACTCTGAAGGGTAGTT+GGG	0.428150	5:-13502136	None:intergenic
CATGGTTATTCAGCACCTCC+TGG	0.431962	5:+13502329	MsaT025853.1:CDS
TACAATGTCAAGTCAGGAAG+AGG	0.434728	5:+13502889	MsaT025853.1:CDS
TCTTTAAGAGCTCGTGAGAA+TGG	0.434908	5:+13502155	MsaT025853.1:CDS
ACCTCAACCTGATCAATATG+AGG	0.437745	5:+13502388	MsaT025853.1:CDS
GTCTTGAGGGGACTTGTTGG+TGG	0.443401	5:-13502078	None:intergenic
AGGTGGGGTGGCTGTTGATG+TGG	0.445640	5:-13502369	None:intergenic
CATCAGAGGGAGGGTCTTGA+GGG	0.445658	5:-13502091	None:intergenic
CCCTCATATTGATCAGGTTG+AGG	0.453934	5:-13502389	None:intergenic
GCAAACATGTGTTTCCCACT+TGG	0.463748	5:+13502258	MsaT025853.1:CDS
CTTTAAGAGCTCGTGAGAAT+GGG	0.465502	5:+13502156	MsaT025853.1:CDS
GCCTTCCTTCCTCTGGTCCA+GGG	0.471276	5:-13502937	None:intergenic
AAGTGATGGTCAAGCTATGA+AGG	0.473835	5:+13502205	MsaT025853.1:CDS
AAGCCAGCCAATAGAGATAG+AGG	0.474391	5:+13502859	MsaT025853.1:CDS
GCTGTTGATGTGGAAGGAAG+TGG	0.478632	5:-13502359	None:intergenic
CTCTGATACAATGTCAAGTC+AGG	0.478904	5:+13502883	MsaT025853.1:CDS
CATATTGATCAGGTTGAGGT+GGG	0.500892	5:-13502385	None:intergenic
AGTCCTCTATCTCTATTGGC+TGG	0.503791	5:-13502862	None:intergenic
TCATCAGAGGGAGGGTCTTG+AGG	0.508193	5:-13502092	None:intergenic
ACTCTGAAGGGTAGTTGGGT+TGG	0.513668	5:-13502132	None:intergenic
AGTTGGGTTGGTTTCTTTGG+GGG	0.515909	5:-13502120	None:intergenic
AGCTCGTGAGAATGGGAAGC+TGG	0.517710	5:+13502163	MsaT025853.1:CDS
ACCCTGGACCAGAGGAAGGA+AGG	0.521179	5:+13502936	MsaT025853.1:CDS
CAAACATGTGTTTCCCACTT+GGG	0.531651	5:+13502259	MsaT025853.1:CDS
GCTTATCAGTTGTATCGGCT+GGG	0.531837	5:-13502980	None:intergenic
TATATTTGTTGTCATCCTCA+TGG	0.532111	5:+13502311	MsaT025853.1:CDS
TTGATCAGGTTGAGGTGGGG+TGG	0.544768	5:-13502381	None:intergenic
TCATATTGATCAGGTTGAGG+TGG	0.558741	5:-13502386	None:intergenic
GAGCTGCTTATCAGTTGTAT+CGG	0.559761	5:-13502985	None:intergenic
GAGCTCTTAAAGAACTCTGA+AGG	0.564348	5:-13502145	None:intergenic
GGGGTGGCTGTTGATGTGGA+AGG	0.586960	5:-13502365	None:intergenic
ATATTGATCAGGTTGAGGTG+GGG	0.595131	5:-13502384	None:intergenic
CAGAGGAAGGAAGGCCCAAG+AGG	0.596615	5:+13502945	MsaT025853.1:CDS
CAGGAAGAGGGAAGGAACCA+AGG	0.600368	5:+13502902	MsaT025853.1:CDS
AGTGATGGTCAAGCTATGAA+GGG	0.603559	5:+13502206	MsaT025853.1:CDS
CAAGGTGCAAACAGTAACCC+TGG	0.607882	5:+13502920	MsaT025853.1:CDS
AATGGGAAGCTGGCAGCTGA+TGG	0.610197	5:+13502173	MsaT025853.1:CDS
GGGGATTCATCATCAGAGGG+AGG	0.610326	5:-13502101	None:intergenic
AAGCTGGCAGCTGATGGCGA+AGG	0.613924	5:+13502179	MsaT025853.1:CDS
AGTAACCCTGGACCAGAGGA+AGG	0.621380	5:+13502932	MsaT025853.1:CDS
AGCTCTTAAAGAACTCTGAA+GGG	0.626255	5:-13502144	None:intergenic
CTTCATCAAAGTAGCCCAAG+TGG	0.635469	5:-13502273	None:intergenic
ATGGTCAAGCTATGAAGGGG+AGG	0.636182	5:+13502210	MsaT025853.1:CDS
ACAATGTCAAGTCAGGAAGA+GGG	0.642713	5:+13502890	MsaT025853.1:CDS
CCTCAACCTGATCAATATGA+GGG	0.650750	5:+13502389	MsaT025853.1:CDS
ATCAGAGGGAGGGTCTTGAG+GGG	0.650850	5:-13502090	None:intergenic
AAACAGTAACCCTGGACCAG+AGG	0.653653	5:+13502928	MsaT025853.1:CDS
TTCATCAAAGTAGCCCAAGT+GGG	0.657037	5:-13502272	None:intergenic
TGTCAAGTCAGGAAGAGGGA+AGG	0.657401	5:+13502894	MsaT025853.1:CDS
GATGGCGAAGGAGTAAGTGA+TGG	0.658846	5:+13502191	MsaT025853.1:CDS
GTGATGGTCAAGCTATGAAG+GGG	0.659294	5:+13502207	MsaT025853.1:CDS
GGGATTCATCATCAGAGGGA+GGG	0.659890	5:-13502100	None:intergenic
GGAGGTGCTGAATAACCATG+AGG	0.763451	5:-13502326	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	13502035	13502997	13502035	ID=MsaG025853
Chr5	mRNA	13502035	13502997	13502035	ID=MsaT025853.1;Parent=MsaG025853
Chr5	exon	13502035	13502410	13502035	ID=MsaT025853.1.exon1;Parent=MsaT025853.1
Chr5	CDS	13502035	13502410	13502035	ID=cds.MsaT025853.1;Parent=MsaT025853.1
Chr5	exon	13502849	13502997	13502849	ID=MsaT025853.1.exon2;Parent=MsaT025853.1
Chr5	CDS	13502849	13502997	13502849	ID=cds.MsaT025853.1;Parent=MsaT025853.1

Gene Sequence

>MsaT025853.1

ATGCTGAACCCTAAACCTAACCCTTCCAAGAATTCCACCCCCACCACCAACAAGTCCCCTCAAGACCCTCCCTCTGATGATGAATCCCCCAAAGAAACCAACCCAACTACCCTTCAGAGTTCTTTAAGAGCTCGTGAGAATGGGAAGCTGGCAGCTGATGGCGAAGGAGTAAGTGATGGTCAAGCTATGAAGGGGAGGAAGAGTAATATTTGTTGTCATAGATGCAAACATGTGTTTCCCACTTGGGCTACTTTGATGAAGCATTATCAAGAACACTATATTTGTTGTCATCCTCATGGTTATTCAGCACCTCCTGGTTTTTCTCCACTTCCTTCCACATCAACAGCCACCCCACCTCAACCTGATCAATATGAGGGTGATGATCAAAGCCAGCCAATAGAGATAGAGGACTCTGATACAATGTCAAGTCAGGAAGAGGGAAGGAACCAAGGTGCAAACAGTAACCCTGGACCAGAGGAAGGAAGGCCCAAGAGGACGATCAAGAAGCCCAGCCGATACAACTGA

Protein sequence

>MsaT025853.1

MLNPKPNPSKNSTPTTNKSPQDPPSDDESPKETNPTTLQSSLRARENGKLAADGEGVSDGQAMKGRKSNICCHRCKHVFPTWATLMKHYQEHYICCHPHGYSAPPGFSPLPSTSTATPPQPDQYEGDDQSQPIEIEDSDTMSSQEEGRNQGANSNPGPEEGRPKRTIKKPSRYN*