Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG025857 | AES95290.1 | 74.408 | 211 | 24 | 3 | 1 | 201 | 1 | 191 | 4.27e-100 | 298 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG025857 | tr|G7K023|G7K023_MEDTR | 74.408 | 211 | 24 | 3 | 1 | 201 | 1 | 191 | 2.04e-100 | 298 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG025857 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG025857 | MtrunA17_Chr5g0406781 | 56.604 | 159 | 29 | 4 | 1 | 159 | 1 | 119 | 8.07e-51 | 160 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 68 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTAGTTGGGTTGGTTTCTT+TGG | 0.162115 | 5:-13546129 | None:intergenic |
| CCTTCAAAGTTCTTTAAGTA+TGG | 0.240152 | 5:+13546151 | MsaT025857.1:CDS |
| TTCTCCGAAGGGTTAGGTTT+AGG | 0.240433 | 5:-13546050 | None:intergenic |
| TCTCCGAAGGGTTAGGTTTA+GGG | 0.249699 | 5:-13546049 | None:intergenic |
| TGTTTGCATCTATAACAATT+TGG | 0.250606 | 5:-13546335 | None:intergenic |
| GTAGTTGGGTTGGTTTCTTT+GGG | 0.266039 | 5:-13546128 | None:intergenic |
| AATGGTTTGTCAGCAGCTTC+TGG | 0.287143 | 5:+13546422 | MsaT025857.1:CDS |
| TTGAGGGGACTTGTTGTTGG+TGG | 0.289994 | 5:-13546081 | None:intergenic |
| TCTGATGAAGGCGAAGGTTT+AGG | 0.328241 | 5:+13547001 | MsaT025857.1:CDS |
| ATACATCACTCTTCTTCATT+AGG | 0.332064 | 5:-13547046 | None:intergenic |
| GTCTTGAGGGGACTTGTTGT+TGG | 0.343036 | 5:-13546084 | None:intergenic |
| TAGTTGGGTTGGTTTCTTTG+GGG | 0.344689 | 5:-13546127 | None:intergenic |
| GTTGAATTCTCCGAAGGGTT+AGG | 0.378551 | 5:-13546056 | None:intergenic |
| CTCTTCCTCCTCTTCATAGC+TGG | 0.393408 | 5:-13546275 | None:intergenic |
| AAAGAACTTTGAAGGGTAGT+TGG | 0.393444 | 5:-13546143 | None:intergenic |
| TGAGGGGACTTGTTGTTGGT+GGG | 0.402381 | 5:-13546080 | None:intergenic |
| AGGGGACTTGTTGTTGGTGG+GGG | 0.408399 | 5:-13546078 | None:intergenic |
| AGGTGGGGTGGTTGTTGATG+TGG | 0.426505 | 5:-13546462 | None:intergenic |
| AAGAACTTTGAAGGGTAGTT+GGG | 0.431057 | 5:-13546142 | None:intergenic |
| GAGTTCAGAGCTCGTGAGAA+TGG | 0.435144 | 5:+13546215 | MsaT025857.1:CDS |
| GACTGCTTTGATGAACCATA+TGG | 0.435911 | 5:+13546373 | MsaT025857.1:CDS |
| ACCTCAACCTGATCAATATG+AGG | 0.437745 | 5:+13546481 | MsaT025857.1:CDS |
| TAACAATTTGGATATATAGA+AGG | 0.438362 | 5:-13546323 | None:intergenic |
| CATCAGAGGGAGGGTCTTGA+GGG | 0.445658 | 5:-13546097 | None:intergenic |
| CCATACTTAAAGAACTTTGA+AGG | 0.446994 | 5:-13546151 | None:intergenic |
| CCCTCATATTGATCAGGTTG+AGG | 0.453934 | 5:-13546482 | None:intergenic |
| GAAGCTGCTGACAAACCATT+AGG | 0.476282 | 5:-13546419 | None:intergenic |
| GCAAACATGTGTTTCCCACT+TGG | 0.483219 | 5:+13546351 | MsaT025857.1:CDS |
| ACTTTGAAGGGTAGTTGGGT+TGG | 0.489348 | 5:-13546138 | None:intergenic |
| GGTCCTCTATCTCCATTGGC+TGG | 0.500299 | 5:-13546934 | None:intergenic |
| CATATTGATCAGGTTGAGGT+GGG | 0.500892 | 5:-13546478 | None:intergenic |
| TCATCAGAGGGAGGGTCTTG+AGG | 0.508193 | 5:-13546098 | None:intergenic |
| AGTTCAGAGCTCGTGAGAAT+GGG | 0.512950 | 5:+13546216 | MsaT025857.1:CDS |
| AGCTCGTGAGAATGGGAAGC+TGG | 0.512973 | 5:+13546223 | MsaT025857.1:CDS |
| AGTTGGGTTGGTTTCTTTGG+GGG | 0.515909 | 5:-13546126 | None:intergenic |
| CATCACTCTTCTTCATTAGG+AGG | 0.517441 | 5:-13547043 | None:intergenic |
| GAGGGGTCCTCTATCTCCAT+TGG | 0.519182 | 5:-13546938 | None:intergenic |
| AAGCCAGCCAATGGAGATAG+AGG | 0.520626 | 5:+13546931 | MsaT025857.1:CDS |
| TTGATCAGGTTGAGGTGGGG+TGG | 0.536734 | 5:-13546474 | None:intergenic |
| GAACCCTAAACCTAACCCTT+CGG | 0.542964 | 5:+13546046 | MsaT025857.1:CDS |
| GAGGGGACTTGTTGTTGGTG+GGG | 0.544991 | 5:-13546079 | None:intergenic |
| AATGGGAAGCTGGCAGCTGA+TGG | 0.545369 | 5:+13546233 | MsaT025857.1:CDS |
| TCAAAGTTCTTTAAGTATGG+AGG | 0.546799 | 5:+13546154 | MsaT025857.1:CDS |
| CATACTTAAAGAACTTTGAA+GGG | 0.549185 | 5:-13546150 | None:intergenic |
| TCATATTGATCAGGTTGAGG+TGG | 0.558741 | 5:-13546479 | None:intergenic |
| TGATGATGAAAGCCAGCCAA+TGG | 0.578386 | 5:+13546922 | MsaT025857.1:CDS |
| GAGTAATATTCGTGAAGTGA+AGG | 0.580783 | 5:+13546295 | MsaT025857.1:CDS |
| CTGGCAGCTGATGGAGAAGG+TGG | 0.580844 | 5:+13546242 | MsaT025857.1:CDS |
| GGGGTGGTTGTTGATGTGGA+AGG | 0.586960 | 5:-13546458 | None:intergenic |
| CTCCATTAAAACTTCGAGAG+CGG | 0.588680 | 5:-13546195 | None:intergenic |
| ATATTGATCAGGTTGAGGTG+GGG | 0.595131 | 5:-13546477 | None:intergenic |
| ATGGTCCAGCTATGAAGAGG+AGG | 0.603822 | 5:+13546270 | MsaT025857.1:CDS |
| CTCGGATGCAACATCATCAG+AGG | 0.604023 | 5:-13546957 | None:intergenic |
| GGGGATTCATCATCAGAGGG+AGG | 0.610326 | 5:-13546107 | None:intergenic |
| TCGGATGCAACATCATCAGA+GGG | 0.613962 | 5:-13546956 | None:intergenic |
| GATGGAGAAGGTGGAAGTGA+TGG | 0.614959 | 5:+13546251 | MsaT025857.1:CDS |
| ACTGCATCTGAGTCTGATGA+AGG | 0.623088 | 5:+13546989 | MsaT025857.1:CDS |
| AATTTGGATATATAGAAGGA+TGG | 0.631395 | 5:-13546319 | None:intergenic |
| TCTGAGTCTGATGAAGGCGA+AGG | 0.633192 | 5:+13546995 | MsaT025857.1:CDS |
| GTGATGGTCCAGCTATGAAG+AGG | 0.637063 | 5:+13546267 | MsaT025857.1:CDS |
| AAGCTGGCAGCTGATGGAGA+AGG | 0.643145 | 5:+13546239 | MsaT025857.1:CDS |
| AGATGCAGTATCATCAGACT+CGG | 0.649465 | 5:-13546975 | None:intergenic |
| CCTCAACCTGATCAATATGA+GGG | 0.650750 | 5:+13546482 | MsaT025857.1:CDS |
| ATCAGAGGGAGGGTCTTGAG+GGG | 0.650850 | 5:-13546096 | None:intergenic |
| TTCATCAAAGCAGTCCAAGT+GGG | 0.656120 | 5:-13546365 | None:intergenic |
| GGGATTCATCATCAGAGGGA+GGG | 0.659890 | 5:-13546106 | None:intergenic |
| GTTCATCAAAGCAGTCCAAG+TGG | 0.685541 | 5:-13546366 | None:intergenic |
| CGGATGCAACATCATCAGAG+GGG | 0.768604 | 5:-13546955 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 13546041 | 13547063 | 13546041 | ID=MsaG025857 |
| Chr5 | mRNA | 13546041 | 13547063 | 13546041 | ID=MsaT025857.1;Parent=MsaG025857 |
| Chr5 | exon | 13546041 | 13546503 | 13546041 | ID=MsaT025857.1.exon1;Parent=MsaT025857.1 |
| Chr5 | CDS | 13546041 | 13546503 | 13546041 | ID=cds.MsaT025857.1;Parent=MsaT025857.1 |
| Chr5 | exon | 13546921 | 13547063 | 13546921 | ID=MsaT025857.1.exon2;Parent=MsaT025857.1 |
| Chr5 | CDS | 13546921 | 13547063 | 13546921 | ID=cds.MsaT025857.1;Parent=MsaT025857.1 |
| Gene Sequence |
| Protein sequence |
