AlfalfaGEDB | MsaG025902

M. sativa ssp. caerulea / MsaG025902

KEGG: -
GO: GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032991 GO:0033558 GO:0035601 GO:0036211 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140096 GO:0140097 GO:1901363 GO:1901564 GO:1902494 GO:1903506 GO:1904949 GO:2000112 GO:2001141
PFAM: PF16135.8

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025902	XP_003612379.1	77.103	428	40	4	1	370	1	428	0.0	637

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025902	sp\|F4IXE7\|IDM1_ARATH	28.205	429	224	7	3	367	621	1029	1.20e-44	168

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025902	tr\|G7K198\|G7K198_MEDTR	77.103	428	40	4	1	370	1	428	0.0	637

TFs/TRs:

Gene ID	Type	Classification
MsaG025902	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG004086	MsaG025902	0.815483	1.564520e-51	3.061463e-49
MsaG004712	MsaG025902	0.809024	3.959241e-50	6.603874e-48
MsaG006476	MsaG025902	0.800898	1.943953e-48	2.682097e-46
MsaG009871	MsaG025902	0.813149	5.100456e-51	9.411981e-49
MsaG012396	MsaG025902	0.818210	3.849678e-52	8.078860e-50
MsaG018437	MsaG025902	0.801056	1.805419e-48	2.499821e-46
MsaG018682	MsaG025902	0.805915	1.795045e-49	2.780051e-47
MsaG023997	MsaG025902	0.803260	6.386929e-49	9.300302e-47
MsaG025902	MsaG028585	0.817395	5.868289e-52	1.205911e-49
MsaG025902	MsaG029403	0.811746	1.029776e-50	1.835251e-48
MsaG025902	MsaG030337	0.804533	3.482931e-49	5.223449e-47
MsaG025902	MsaG030353	0.807405	8.726416e-50	1.399974e-47
MsaG025902	MsaG030665	0.803225	6.492822e-49	9.446990e-47
MsaG025902	MsaG035553	0.803604	5.423557e-49	7.960470e-47
MsaG025902	MsaG045923	0.811407	1.219140e-50	2.154774e-48
MsaG025902	MsaG045994	0.814916	2.088388e-51	4.028459e-49
MsaG025902	MsaG046278	0.804931	2.879844e-49	4.358982e-47
MsaG025902	MsaG046755	0.806794	1.173914e-49	1.856268e-47
MsaG025902	MsaG000821	0.800905	1.936958e-48	2.672910e-46
MsaG025902	MsaG006009	0.809830	2.663321e-50	4.529620e-48
MsaG025902	MsaG038133	0.808109	6.192699e-50	1.010440e-47
MsaG025902	MsaG036344	0.819940	1.561662e-52	3.429223e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025902	MtrunA17_Chr5g0407211	77.103	428	40	4	1	370	1	428	0.0	637
MsaG025902	MtrunA17_Chr5g0407251	75.935	428	45	4	1	370	1	428	0.0	627
MsaG025902	MtrunA17_Chr3g0134811	56.897	464	102	7	3	370	339	800	1.42e-171	498
MsaG025902	MtrunA17_Chr3g0134821	56.036	439	101	4	2	370	425	841	1.14e-160	471
MsaG025902	MtrunA17_Chr7g0270101	57.783	424	112	6	3	370	384	796	1.51e-157	462
MsaG025902	MtrunA17_Chr5g0430361	37.762	429	178	8	3	363	652	1059	1.10e-86	283
MsaG025902	MtrunA17_Chr3g0134851	37.762	429	131	7	2	363	107	466	2.80e-76	243
MsaG025902	MtrunA17_Chr3g0105291	37.073	410	207	12	1	361	601	1008	6.93e-71	239
MsaG025902	MtrunA17_Chr2g0316251	29.274	427	212	7	3	361	762	1166	1.16e-51	185
MsaG025902	MtrunA17_Chr1g0205451	30.025	403	190	8	3	335	606	986	4.40e-41	154
MsaG025902	MtrunA17_Chr7g0259981	28.409	264	136	5	125	340	13	271	3.69e-33	125
MsaG025902	MtrunA17_Chr1g0185491	32.558	215	133	3	138	340	985	1199	4.71e-29	119
MsaG025902	MtrunA17_Chr1g0185491	30.952	168	92	4	3	166	812	959	7.60e-14	73.2
MsaG025902	MtrunA17_Chr1g0179841	22.770	426	223	16	37	363	386	804	1.36e-18	87.4
MsaG025902	MtrunA17_Chr7g0265211	24.373	279	168	7	127	368	678	950	1.64e-18	87.4
MsaG025902	MtrunA17_Chr2g0320451	30.556	180	116	4	187	365	852	1023	2.39e-18	87.0
MsaG025902	MtrunA17_Chr1g0182481	22.633	433	242	15	4	361	478	892	4.30e-18	86.3
MsaG025902	MtrunA17_Chr4g0025251	29.605	152	104	2	215	364	554	704	3.41e-13	70.9
MsaG025902	MtrunA17_Chr4g0028111	31.783	129	87	1	208	336	435	562	9.59e-13	69.3
MsaG025902	MtrunA17_Chr3g0139561	28.947	190	103	7	153	332	188	355	1.07e-11	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025902	AT5G58610.1	37.284	405	187	7	3	361	590	973	1.44e-71	241
MsaG025902	AT5G58610.6	37.284	405	187	7	3	361	590	973	1.45e-71	241
MsaG025902	AT5G58610.4	34.483	435	184	11	3	361	368	777	1.72e-63	217
MsaG025902	AT5G58610.8	34.483	435	184	11	3	361	599	1008	6.85e-63	217
MsaG025902	AT5G58610.2	34.483	435	184	11	3	361	599	1008	6.85e-63	217
MsaG025902	AT5G58610.5	34.483	435	184	11	3	361	590	999	8.90e-63	217
MsaG025902	AT5G58610.3	34.483	435	184	11	3	361	599	1008	1.00e-62	217
MsaG025902	AT5G58610.7	34.483	435	184	11	3	361	590	999	1.35e-62	216
MsaG025902	AT5G63900.1	33.642	324	152	7	94	363	228	542	1.45e-48	172
MsaG025902	AT5G58610.10	33.420	383	154	11	3	309	599	956	2.96e-48	175
MsaG025902	AT5G58610.9	33.420	383	154	11	3	309	590	947	3.39e-48	175
MsaG025902	AT1G05380.3	29.577	426	213	8	3	361	520	925	5.50e-46	169
MsaG025902	AT1G05380.1	29.577	426	213	8	3	361	520	925	5.50e-46	169
MsaG025902	AT1G05380.2	29.577	426	213	8	3	361	520	925	5.50e-46	169
MsaG025902	AT3G14980.3	28.205	429	224	7	3	367	621	1029	8.75e-46	168
MsaG025902	AT3G14980.6	28.205	429	224	7	3	367	621	1029	1.22e-45	168
MsaG025902	AT3G14980.1	28.205	429	224	7	3	367	621	1029	1.22e-45	168
MsaG025902	AT3G14980.2	28.205	429	224	7	3	367	621	1029	1.22e-45	168
MsaG025902	AT3G14980.5	28.205	429	224	7	3	367	576	984	1.29e-45	167
MsaG025902	AT5G58610.11	34.302	344	127	9	3	271	590	909	3.13e-44	163
MsaG025902	AT4G14920.2	27.166	427	226	6	3	364	580	986	5.80e-43	160
MsaG025902	AT4G14920.1	27.166	427	226	6	3	364	580	986	5.80e-43	160
MsaG025902	AT4G14920.3	27.166	427	226	6	3	364	580	986	6.44e-43	160
MsaG025902	AT5G36740.5	26.824	425	225	5	3	361	545	949	2.90e-41	155
MsaG025902	AT5G36740.1	26.824	425	225	5	3	361	545	949	2.90e-41	155
MsaG025902	AT5G36670.4	24.943	441	227	7	3	361	545	963	3.72e-33	131
MsaG025902	AT5G36670.5	24.943	441	227	7	3	361	545	963	3.72e-33	131
MsaG025902	AT5G36670.2	25.886	367	186	5	3	303	545	891	2.35e-31	126
MsaG025902	AT5G36740.2	25.886	367	186	5	3	303	545	891	2.35e-31	126
MsaG025902	AT5G36740.3	25.886	367	186	5	3	303	545	891	2.35e-31	126
MsaG025902	AT5G36740.4	34.158	202	127	1	166	361	605	806	1.52e-30	124
MsaG025902	AT5G36740.4	30.814	172	95	3	3	170	392	543	3.30e-17	84.0
MsaG025902	AT3G14980.4	26.048	334	163	7	3	272	621	934	1.09e-24	106
MsaG025902	AT5G36670.3	29.817	218	129	3	166	361	605	820	2.68e-22	99.4
MsaG025902	AT5G36670.3	30.814	172	95	3	3	170	392	543	2.53e-17	84.3
MsaG025902	AT5G36670.1	29.817	218	129	3	166	361	758	973	4.31e-22	98.6
MsaG025902	AT5G36670.1	31.056	161	89	2	3	161	545	685	3.55e-17	84.0
MsaG025902	AT2G36720.1	26.718	262	157	10	133	365	718	973	1.66e-19	90.9
MsaG025902	AT2G36720.2	26.718	262	157	10	133	365	718	973	1.66e-19	90.9
MsaG025902	AT2G37520.1	27.092	251	144	7	140	359	570	812	5.95e-18	86.3
MsaG025902	AT3G53680.2	40.336	119	66	2	221	336	691	807	1.71e-17	84.7
MsaG025902	AT3G53680.1	40.336	119	66	2	221	336	689	805	1.86e-17	84.7
MsaG025902	AT2G27980.1	29.487	156	107	2	210	364	907	1060	1.88e-16	81.6

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATTATCTGCAACCTTAAT+TGG	0.181951	5:+14197992	None:intergenic
GTTTGTTCAGTTTGTGGTTT+TGG	0.236361	5:-14198320	MsaT025902.1:CDS
TGGTTTCTATAATGTGATTT+TGG	0.241530	5:-14197777	MsaT025902.1:CDS
GTGGCAACTATAAGAATTTA+TGG	0.245404	5:-14197731	MsaT025902.1:CDS
ATCAACTCTCTTCCTGATAA+AGG	0.280267	5:+14197851	None:intergenic
GCATTCCACCTCCGGCGTTT+AGG	0.305435	5:-14198263	MsaT025902.1:CDS
GTTGATAAAGAATCAAGTTT+TGG	0.324712	5:-14198684	MsaT025902.1:CDS
TTATGTCTAAAGAAGTTGTT+AGG	0.347395	5:-14198019	MsaT025902.1:CDS
AAAGGAGAGTAAGTTGAATA+TGG	0.354999	5:-14197915	MsaT025902.1:CDS
AAACGATGGGTATTGTTTCT+TGG	0.373623	5:-14198706	None:intergenic
GATAGTGTTTGTTCAGTTTG+TGG	0.378867	5:-14198326	MsaT025902.1:CDS
CTGGGTTCGCATGATCATAT+AGG	0.387880	5:-14198455	MsaT025902.1:CDS
GGATTGTCAAAGGGAAGCTC+TGG	0.400874	5:-14198474	MsaT025902.1:CDS
AACACTTGGACAAGATCATT+TGG	0.403595	5:-14197560	MsaT025902.1:CDS
TGAAGAGGAGTTTAATCAAA+AGG	0.404213	5:-14197933	MsaT025902.1:CDS
AAGCACTGAAAAGACCTATA+TGG	0.410177	5:-14197454	MsaT025902.1:CDS
GTCCGTCTGCATTCCACCTC+CGG	0.420321	5:-14198271	MsaT025902.1:CDS
GGCTCTTGGTGTATTGTATG+AGG	0.423950	5:-14197894	MsaT025902.1:CDS
ACCATCCACAAGTATCTTGT+TGG	0.424383	5:-14198516	MsaT025902.1:CDS
GTTGTTAGGAAAACCAATTA+AGG	0.435095	5:-14198005	MsaT025902.1:CDS
TATAAGAATTTATGGTCAAA+AGG	0.441015	5:-14197723	MsaT025902.1:CDS
AGTAACAAAGTTGTCAAAAG+AGG	0.441397	5:-14198632	MsaT025902.1:CDS
ATCGAGTTCCGCTTGGTGAA+TGG	0.464422	5:-14198235	MsaT025902.1:intron
CAAGCAATGCAAGGCCATAT+AGG	0.465235	5:+14197440	None:intergenic
CCTGATAAAGGATCAAAAGT+TGG	0.477884	5:+14197863	None:intergenic
ACAAGTATCTTGTTGGAAGA+TGG	0.480189	5:-14198509	MsaT025902.1:CDS
TTGTCTTCGATACTGTTCCT+TGG	0.482098	5:+14197676	None:intergenic
TGAGACCTAAACGCCGGAGG+TGG	0.487780	5:+14198258	None:intergenic
GGAAGATGGAAGGTCGTTGT+TGG	0.488980	5:-14198495	MsaT025902.1:CDS
TGGGTTCGCATGATCATATA+GGG	0.493127	5:-14198454	MsaT025902.1:CDS
GCTCTTGGTGTATTGTATGA+GGG	0.493509	5:-14197893	MsaT025902.1:CDS
GAGAAACAGTTAACTCGCTT+AGG	0.495020	5:-14197623	MsaT025902.1:CDS
AGGAATGTGTCATTTGTTGA+TGG	0.496868	5:-14197654	MsaT025902.1:CDS
CAGAGGATGCTATGAACACT+TGG	0.500216	5:-14197574	MsaT025902.1:CDS
GTCGTTGTTGGATTGTCAAA+GGG	0.517227	5:-14198483	MsaT025902.1:CDS
TCTTCGATACTGTTCCTTGG+TGG	0.524421	5:+14197679	None:intergenic
GGTCGTTGTTGGATTGTCAA+AGG	0.528790	5:-14198484	MsaT025902.1:CDS
AGTAAGTTGAATATGGCTCT+TGG	0.540429	5:-14197908	MsaT025902.1:CDS
ATCTTCCAACAAGATACTTG+TGG	0.550941	5:+14198511	None:intergenic
GGTCTCAATCGAGTTCCGCT+TGG	0.551276	5:-14198242	MsaT025902.1:CDS
TGTGAATAGTGTTGATGAAG+AGG	0.557314	5:-14197948	MsaT025902.1:CDS
CACCGGCTTCGAGTCTCATG+CGG	0.565673	5:-14198555	MsaT025902.1:CDS
ACCGGCTTCGAGTCTCATGC+GGG	0.580291	5:-14198554	MsaT025902.1:CDS
GTAACAAAGTTGTCAAAAGA+GGG	0.581494	5:-14198631	MsaT025902.1:CDS
GATTGTCAAAGGGAAGCTCT+GGG	0.597706	5:-14198473	MsaT025902.1:CDS
TTTCAGTGCTTTGAGACACA+TGG	0.604056	5:+14197466	None:intergenic
AGATAATCTAACATGGACAT+TGG	0.607457	5:-14197978	MsaT025902.1:CDS
CTCCAACATCAAGCAATGCA+AGG	0.609626	5:+14197431	None:intergenic
TCCAACAAGATACTTGTGGA+TGG	0.616702	5:+14198515	None:intergenic
CGCCGGAGGTGGAATGCAGA+CGG	0.624832	5:+14198269	None:intergenic
ACCCGCATGAGACTCGAAGC+CGG	0.625739	5:+14198553	None:intergenic
GTATCTTGTTGGAAGATGGA+AGG	0.629827	5:-14198505	MsaT025902.1:CDS
ATTACTTCTGCATTCCTCAG+AGG	0.633654	5:-14197591	MsaT025902.1:CDS
AATGGGTGAAGTGATTTCAG+TGG	0.642896	5:-14197750	MsaT025902.1:CDS
AAATCACATTATAGAAACCA+CGG	0.660195	5:+14197780	None:intergenic
AGGTTGCAGATAATCTAACA+TGG	0.665981	5:-14197985	MsaT025902.1:CDS
CTCGATTGAGACCTAAACGC+CGG	0.666414	5:+14198252	None:intergenic
GGACAAAACCATTCACCAAG+CGG	0.675521	5:+14198227	None:intergenic
AAGGAACAGTATCGAAGACA+AGG	0.678537	5:-14197674	MsaT025902.1:CDS
ACTACCCTGACAGAAAACAT+TGG	0.690433	5:+14198652	None:intergenic
GATTGAGACCTAAACGCCGG+AGG	0.716742	5:+14198255	None:intergenic
AGTGTTCATAGCATCCTCTG+AGG	0.736105	5:+14197577	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	14197439	14198723	14197439	ID=MsaG025902
Chr5	mRNA	14197439	14198723	14197439	ID=MsaT025902.1;Parent=MsaG025902
Chr5	exon	14197439	14198063	14197439	ID=MsaT025902.1.exon2;Parent=MsaT025902.1
Chr5	CDS	14197439	14198063	14197439	ID=cds.MsaT025902.1;Parent=MsaT025902.1
Chr5	exon	14198236	14198723	14198236	ID=MsaT025902.1.exon1;Parent=MsaT025902.1
Chr5	CDS	14198236	14198723	14198236	ID=cds.MsaT025902.1;Parent=MsaT025902.1

Gene Sequence

>MsaT025902.1

ATGGGTATTGTTTCTTGGTTGATAAAGAATCAAGTTTTGGTTTCAGGGACCAATGTTTTCTGTCAGGGTAGTAACAAAGTTGTCAAAAGAGGGAGTTTGTTTTCTGATGCTATAGTCTGTGATTGTTGTCGTGTTACTTTTACTATCACCGGCTTCGAGTCTCATGCGGGTTGTACAAGACATAGACCATCCACAAGTATCTTGTTGGAAGATGGAAGGTCGTTGTTGGATTGTCAAAGGGAAGCTCTGGGTTCGCATGATCATATAGGGAATCATTGTGTTGTCAAAGAAAATCAAAAGAAGAATCATTCTATTGTCAAAGAAAATCGAAAGAAGAATCATTGTGCTGTCAAAGAAAAATCTGAAGCAAATAACGATAGTGTTTGTTCAGTTTGTGGTTTTGGAGGTGACTTGGCACTATGCGATCGATGTCCGTCTGCATTCCACCTCCGGCGTTTAGGTCTCAATCGAGTTCCGCTTGGTGAATGTGTAGTTTGTGGGAACATGTTCTTATGTCTAAAGAAGTTGTTAGGAAAACCAATTAAGGTTGCAGATAATCTAACATGGACATTGGTGAAGAATGTGAATAGTGTTGATGAAGAGGAGTTTAATCAAAAGGAGAGTAAGTTGAATATGGCTCTTGGTGTATTGTATGAGGGTTTTAATCCAACTTTTGATCCTTTATCAGGAAGAGAGTTGATTAAAGATGTTGTTTTTAGTAGAGAATCAGAACAAAAACGCTTAAACTTCCGTGGTTTCTATAATGTGATTTTGGAGAAAATGGGTGAAGTGATTTCAGTGGCAACTATAAGAATTTATGGTCAAAAGGTTGCAGAGATAGTTTTCGTGGCCACCAAGGAACAGTATCGAAGACAAGGAATGTGTCATTTGTTGATGGATGAGATTGAGAAACAGTTAACTCGCTTAGGAGTTGAGAAATTACTTCTGCATTCCTCAGAGGATGCTATGAACACTTGGACAAGATCATTTGGTTTTGCAAGGATGAATAGTAAAGATAAGTGTCAGTTTATTGATCATACTTTTTTAGAATTTGAGAATGGTACCATGTGTCTCAAAGCACTGAAAAGACCTATATGGCCTTGCATTGCTTGA

Protein sequence

>MsaT025902.1

MGIVSWLIKNQVLVSGTNVFCQGSNKVVKRGSLFSDAIVCDCCRVTFTITGFESHAGCTRHRPSTSILLEDGRSLLDCQREALGSHDHIGNHCVVKENQKKNHSIVKENRKKNHCAVKEKSEANNDSVCSVCGFGGDLALCDRCPSAFHLRRLGLNRVPLGECVVCGNMFLCLKKLLGKPIKVADNLTWTLVKNVNSVDEEEFNQKESKLNMALGVLYEGFNPTFDPLSGRELIKDVVFSRESEQKRLNFRGFYNVILEKMGEVISVATIRIYGQKVAEIVFVATKEQYRRQGMCHLLMDEIEKQLTRLGVEKLLLHSSEDAMNTWTRSFGFARMNSKDKCQFIDHTFLEFENGTMCLKALKRPIWPCIA*