AlfalfaGEDB | MsaG025928

M. sativa ssp. caerulea / MsaG025928

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02042.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025928	XP_003612350.1	93.248	311	21	0	1	311	20	330	0.0	595

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025928	sp\|O81791\|RKD5_ARATH	50.485	309	125	8	1	294	59	354	1.10e-85	265

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025928	tr\|G7K041\|G7K041_MEDTR	93.248	311	21	0	1	311	20	330	0.0	595

TFs/TRs:

Gene ID	Type	Classification
MsaG025928	TF	RWP-RK

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000671	MsaG025928	0.807228	9.510968e-50	1.519362e-47
MsaG007554	MsaG025928	0.809017	3.972201e-50	6.624501e-48
MsaG018781	MsaG025928	0.803477	5.760426e-49	8.430165e-47
MsaG019578	MsaG025928	0.801154	1.724475e-48	2.393058e-46
MsaG020946	MsaG025928	0.812039	8.898916e-51	1.597391e-48
MsaG023067	MsaG025928	0.801031	1.826475e-48	2.527604e-46
MsaG024694	MsaG025928	0.800395	2.458769e-48	3.354250e-46
MsaG025928	MsaG029878	0.824133	1.685179e-53	4.139924e-51
MsaG025928	MsaG046426	0.811579	1.119349e-50	1.986744e-48
MsaG025928	MsaG043095	0.818012	4.264264e-52	8.903559e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025928	MtrunA17_Chr5g0406901	93.248	311	21	0	1	311	20	330	0.0	595
MsaG025928	MtrunA17_Chr3g0092141	36.296	135	78	2	162	295	38	165	9.18e-22	90.9
MsaG025928	MtrunA17_Chr4g0060761	36.090	133	81	1	172	304	145	273	1.28e-21	92.4
MsaG025928	MtrunA17_Chr3g0127001	37.600	125	60	2	171	295	148	254	2.84e-19	85.5
MsaG025928	MtrunA17_Chr1g0182631	37.594	133	63	3	163	295	115	227	3.67e-19	84.7
MsaG025928	MtrunA17_Chr3g0136081	47.126	87	41	2	180	264	414	497	4.81e-13	69.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG025928	AT4G35590.2	50.485	309	125	8	1	294	9	304	1.08e-87	265
MsaG025928	AT4G35590.1	50.485	309	125	8	1	294	59	354	1.12e-86	265
MsaG025928	AT1G74480.1	45.217	115	58	2	180	292	131	242	3.79e-22	94.7
MsaG025928	AT1G18790.1	43.802	121	62	3	175	293	120	236	4.95e-22	93.6
MsaG025928	AT5G66990.1	42.727	110	59	2	187	295	164	270	2.22e-20	89.4
MsaG025928	AT5G53040.1	42.609	115	53	1	181	295	147	248	3.17e-20	88.6
MsaG025928	AT5G16100.2	50.000	64	32	0	174	237	346	409	1.90e-13	70.9

Find 58 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATTACTTTATCAGATT+TGG	0.267461	5:+14451811	MsaT025928.1:CDS
AAAAGGAAGTGCAGAGAATT+TGG	0.277802	5:+14451899	MsaT025928.1:CDS
TGAAAGCTACTCCAATTCTT+AGG	0.296460	5:+14449883	MsaT025928.1:CDS
TTTCTCCTCCATCACATTCA+TGG	0.321493	5:-14449809	None:intergenic
CAGTCTCATTCATGATCTTC+AGG	0.329225	5:+14451961	MsaT025928.1:CDS
TTTGGAATGGCCTGAAGCTT+TGG	0.353863	5:-14451276	None:intergenic
AGAAGACGAGTCTGAGTTAC+TGG	0.356355	5:+14451467	MsaT025928.1:CDS
TTTCAGGAATCCAAAGCTTC+AGG	0.357774	5:+14451266	MsaT025928.1:intron
GAGCTCTGCAAGGGAAGAAT+TGG	0.370187	5:+14451365	MsaT025928.1:CDS
ATACCTTGTAACCAACAAAA+AGG	0.370413	5:-14450018	None:intergenic
GATTTCAACAACCTAAGAAT+TGG	0.374799	5:-14449894	None:intergenic
TGGAGATCATTAAGCAGGTT+TGG	0.377748	5:-14451294	None:intergenic
GTATTCCTCGCTGGCCTCAT+AGG	0.379146	5:+14451921	MsaT025928.1:CDS
AAGGGTATAAGAATGGAGTT+TGG	0.381031	5:+14449940	MsaT025928.1:CDS
AGATGCTAATTGCTAGGTTT+AGG	0.384292	5:-14452725	None:intergenic
AGTCTTGCCTGTGCTTAATC+AGG	0.396249	5:+14451422	MsaT025928.1:CDS
TCTTCCCTTGCAGAGCTCTC+TGG	0.396713	5:-14451360	None:intergenic
GAGTTTGTGTTTCCTGAGAA+TGG	0.398574	5:+14449846	MsaT025928.1:CDS
CATAGAGAAGAGGCTCTTCA+TGG	0.402183	5:+14452607	MsaT025928.1:CDS
CGTCAAAGAATCTAAATGTT+GGG	0.449206	5:+14451864	MsaT025928.1:CDS
TTGCCTTCTTATCACTGCCA+TGG	0.457172	5:-14452558	None:intergenic
AAAGATGTTGGAAAGTGAAA+AGG	0.459498	5:+14452580	MsaT025928.1:CDS
ACAGGCAAGACTTTCTTTAG+TGG	0.459764	5:-14451411	None:intergenic
AATTGGCATATCAAAGTACT+TGG	0.464269	5:-14451834	None:intergenic
TTGGGCTTACTGTTCTCAAA+AGG	0.466425	5:+14451882	MsaT025928.1:CDS
AACATCTTGTCTGAATCTCT+TGG	0.478887	5:-14452645	None:intergenic
GCAAGACTTTCTTTAGTGGT+TGG	0.481995	5:-14451407	None:intergenic
TCAGACTCGTCTTCTTCAAA+AGG	0.484709	5:-14451459	None:intergenic
GAGAATTTGGTATTCCTCGC+TGG	0.487057	5:+14451912	MsaT025928.1:CDS
TCAACATAAGAACCATTCTC+AGG	0.492545	5:-14449858	None:intergenic
CAGCCATGGCAGTGATAAGA+AGG	0.499451	5:+14452555	MsaT025928.1:CDS
GCAGGAAGAGGCAAAACATC+AGG	0.500010	5:+14452514	MsaT025928.1:intron
TGAATGTGATGGAGGAGAAA+AGG	0.500666	5:+14449812	MsaT025928.1:CDS
GATAAGAAGGCAAAAGATGT+TGG	0.505266	5:+14452568	MsaT025928.1:CDS
ATCATTAAGCAGGTTTGGAA+TGG	0.506660	5:-14451289	None:intergenic
GCGTCAAAGAATCTAAATGT+TGG	0.518286	5:+14451863	MsaT025928.1:CDS
TCATGTGGAGATCATTAAGC+AGG	0.525296	5:-14451299	None:intergenic
AATCTTTGCAACATGGTCAC+TGG	0.527182	5:-14451795	None:intergenic
TTGGAATGTGAGAAAATTGA+GGG	0.529395	5:-14449995	None:intergenic
ATACAAGTTGGAACCGAAGA+AGG	0.529415	5:+14451323	MsaT025928.1:CDS
AGAGGCAAAACATCAGGAAA+GGG	0.535237	5:+14452520	MsaT025928.1:CDS
ATCAGAAGATGCTAATTGCT+AGG	0.535738	5:-14452731	None:intergenic
GTTGGAATGTGAGAAAATTG+AGG	0.552092	5:-14449996	None:intergenic
TCTCCACATGATATACAAGT+TGG	0.555482	5:+14451311	MsaT025928.1:CDS
AAGAGGCAAAACATCAGGAA+AGG	0.561722	5:+14452519	MsaT025928.1:CDS
GTTCCAACTTGTATATCATG+TGG	0.569302	5:-14451314	None:intergenic
AAAAGGAAAACATAGAGAAG+AGG	0.578123	5:+14452597	MsaT025928.1:CDS
GGAGGATAAAGATGCAGCCA+TGG	0.600209	5:+14452541	MsaT025928.1:CDS
AGTGATTTGATCTTCCTATG+AGG	0.604696	5:-14451935	None:intergenic
TTGAGATCCTGATTAAGCAC+AGG	0.605314	5:-14451429	None:intergenic
GGCAAAACATCAGGAAAGGG+AGG	0.624378	5:+14452523	MsaT025928.1:CDS
TACACCAGAGAGCTCTGCAA+GGG	0.628005	5:+14451356	MsaT025928.1:CDS
ATACACCAGAGAGCTCTGCA+AGG	0.645197	5:+14451355	MsaT025928.1:CDS
CATGTGTACCATGAATGTGA+TGG	0.662518	5:+14449801	MsaT025928.1:CDS
ATAAAGTAATCTTTGCAACA+TGG	0.664575	5:-14451802	None:intergenic
ATCTTCCTATGAGGCCAGCG+AGG	0.669880	5:-14451926	None:intergenic
TCTGCAAGGGAAGAATTGGA+AGG	0.677605	5:+14451369	MsaT025928.1:CDS
GTGTACCATGAATGTGATGG+AGG	0.680494	5:+14449804	MsaT025928.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	14449789	14452742	14449789	ID=MsaG025928
Chr5	mRNA	14449789	14452742	14449789	ID=MsaT025928.1;Parent=MsaG025928
Chr5	exon	14449789	14450036	14449789	ID=MsaT025928.1.exon1;Parent=MsaT025928.1
Chr5	CDS	14449789	14450036	14449789	ID=cds.MsaT025928.1;Parent=MsaT025928.1
Chr5	exon	14451272	14451519	14451272	ID=MsaT025928.1.exon2;Parent=MsaT025928.1
Chr5	CDS	14451272	14451519	14451272	ID=cds.MsaT025928.1;Parent=MsaT025928.1
Chr5	exon	14451768	14451982	14451768	ID=MsaT025928.1.exon3;Parent=MsaT025928.1
Chr5	CDS	14451768	14451982	14451768	ID=cds.MsaT025928.1;Parent=MsaT025928.1
Chr5	exon	14452518	14452742	14452518	ID=MsaT025928.1.exon4;Parent=MsaT025928.1
Chr5	CDS	14452518	14452742	14452518	ID=cds.MsaT025928.1;Parent=MsaT025928.1

Gene Sequence

>MsaT025928.1

ATGAGAAGCGTGCATGTGTACCATGAATGTGATGGAGGAGAAAAGGAAGTTGAAAGAGAGTTTGTGTTTCCTGAGAATGGTTCTTATGTTGAAATGAAAGCTACTCCAATTCTTAGGTTGTTGAAATCTTTTGTTTCTGTAGAGATTTTTGAAGGGTATAAGAATGGAGTTTGGCTTTGTGTTTTTGCTTTTCATGCTTGTAATGTCCCTCAATTTTCTCACATTCCAACCTTTTTGTTGGTTACAAGGAATCCAAAGCTTCAGGCCATTCCAAACCTGCTTAATGATCTCCACATGATATACAAGTTGGAACCGAAGAAGGAACGAAAAGATACACCAGAGAGCTCTGCAAGGGAAGAATTGGAAGGAAACAACAATGATTTCCAACCACTAAAGAAAGTCTTGCCTGTGCTTAATCAGGATCTCAATTGTCTTCCTTTTGAAGAAGACGAGTCTGAGTTACTGGATGATGAGACAGATTTTGAAAGTTCAGCAGGTTTTTTAGGAAAGAAAAAGAGGGCAGCCAGTGACCATGTTGCAAAGATTACTTTATCAGATTTGGCCAAGTACTTTGATATGCCAATTGCAAAAGCGTCAAAGAATCTAAATGTTGGGCTTACTGTTCTCAAAAGGAAGTGCAGAGAATTTGGTATTCCTCGCTGGCCTCATAGGAAGATCAAATCACTCGACAGTCTCATTCATGATCTTCAGGAAGAGGCAAAACATCAGGAAAGGGAGGATAAAGATGCAGCCATGGCAGTGATAAGAAGGCAAAAGATGTTGGAAAGTGAAAAGGAAAACATAGAGAAGAGGCTCTTCATGGACATTCAGATTGAGACCAAGAGATTCAGACAAGATGTTTTCAAGAGAAGGCACAGAGCTAGAGTTATTGAAAACAGAATTCTACAGTTGCAAGCACCTAAACCTAGCAATTAG

Protein sequence

>MsaT025928.1

MRSVHVYHECDGGEKEVEREFVFPENGSYVEMKATPILRLLKSFVSVEIFEGYKNGVWLCVFAFHACNVPQFSHIPTFLLVTRNPKLQAIPNLLNDLHMIYKLEPKKERKDTPESSAREELEGNNNDFQPLKKVLPVLNQDLNCLPFEEDESELLDDETDFESSAGFLGKKKRAASDHVAKITLSDLAKYFDMPIAKASKNLNVGLTVLKRKCREFGIPRWPHRKIKSLDSLIHDLQEEAKHQEREDKDAAMAVIRRQKMLESEKENIEKRLFMDIQIETKRFRQDVFKRRHRARVIENRILQLQAPKPSN*