AlfalfaGEDB | MsaG026054

M. sativa ssp. caerulea / MsaG026054

KEGG: -
GO: -
PFAM: PF08880.14

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026054	XP_003612622.1	90.090	333	24	1	1	333	1	324	0.0	586

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026054	sp\|Q6AWY4\|GRF5_ORYSJ	35.650	331	157	9	17	319	25	327	1.73e-44	158

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026054	tr\|G7K6W0\|G7K6W0_MEDTR	90.090	333	24	1	1	333	1	324	0.0	586

TFs/TRs:

Gene ID	Type	Classification
MsaG026054	TF	GRF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000200	MsaG026054	0.802991	7.254463e-49	1.049832e-46
MsaG000583	MsaG026054	0.807973	6.620035e-50	1.076618e-47
MsaG002065	MsaG026054	0.848455	1.148066e-59	5.902778e-57
MsaG003155	MsaG026054	0.802409	9.555766e-49	1.364526e-46
MsaG003896	MsaG026054	0.812874	5.856837e-51	1.073377e-48
MsaG004186	MsaG026054	0.835825	2.444475e-56	8.392904e-54
MsaG004993	MsaG026054	0.860519	3.841231e-63	3.046604e-60
MsaG005836	MsaG026054	0.803913	4.681427e-49	6.920668e-47
MsaG005942	MsaG026054	0.818954	2.614961e-52	5.595006e-50
MsaG006136	MsaG026054	0.880048	1.596604e-69	2.887669e-66
MsaG006731	MsaG026054	0.806551	1.320211e-49	2.075740e-47
MsaG006909	MsaG026054	0.804917	2.898359e-49	4.385673e-47
MsaG008080	MsaG026054	0.827021	3.511165e-54	9.341383e-52
MsaG008148	MsaG026054	0.850936	2.346880e-60	1.313705e-57
MsaG009004	MsaG026054	0.833780	7.955247e-56	2.569483e-53
MsaG009891	MsaG026054	0.800443	2.403993e-48	3.283119e-46
MsaG011587	MsaG026054	0.809304	3.449454e-50	5.792306e-48
MsaG012425	MsaG026054	0.807077	1.023174e-49	1.628752e-47
MsaG013177	MsaG026054	0.825711	7.176955e-54	1.841196e-51
MsaG017163	MsaG026054	0.803808	4.921336e-49	7.257688e-47
MsaG017248	MsaG026054	0.850896	2.408778e-60	1.346516e-57
MsaG017267	MsaG026054	0.815460	1.582808e-51	3.095564e-49
MsaG017679	MsaG026054	0.832683	1.487363e-55	4.651765e-53
MsaG018305	MsaG026054	0.801117	1.754430e-48	2.432619e-46
MsaG018468	MsaG026054	0.813932	3.437476e-51	6.469168e-49
MsaG018427	MsaG026054	0.801292	1.616056e-48	2.249672e-46
MsaG018642	MsaG026054	0.874298	1.555653e-67	2.169437e-64
MsaG020567	MsaG026054	0.820596	1.106705e-52	2.472658e-50
MsaG022756	MsaG026054	0.814311	2.837703e-51	5.391244e-49
MsaG023633	MsaG026054	0.853564	4.230580e-61	2.596516e-58
MsaG024311	MsaG026054	0.813114	5.191063e-51	9.570732e-49
MsaG024983	MsaG026054	0.805474	2.219377e-49	3.401865e-47
MsaG025074	MsaG026054	0.822135	4.903745e-53	1.141345e-50
MsaG025779	MsaG026054	0.807476	8.430015e-50	1.354720e-47
MsaG026054	MsaG026603	0.819702	1.769285e-52	3.860550e-50
MsaG026054	MsaG027001	0.869695	5.187055e-66	5.934697e-63
MsaG026054	MsaG027046	0.809858	2.625906e-50	4.469027e-48
MsaG026054	MsaG027329	0.840343	1.702628e-57	6.718695e-55
MsaG026054	MsaG027808	0.831174	3.494196e-55	1.045845e-52
MsaG026054	MsaG027970	0.851104	2.105741e-60	1.185569e-57
MsaG026054	MsaG028941	0.809081	3.848333e-50	6.427805e-48
MsaG026054	MsaG030113	0.811014	1.482473e-50	2.595038e-48
MsaG026054	MsaG030171	0.803846	4.833745e-49	7.134666e-47
MsaG026054	MsaG030366	0.830712	4.532211e-55	1.338562e-52
MsaG026054	MsaG030367	0.873516	2.849841e-67	3.839911e-64
MsaG026054	MsaG030368	0.841682	7.608542e-58	3.132032e-55
MsaG026054	MsaG031143	0.876914	1.992614e-68	3.121213e-65
MsaG026054	MsaG031479	0.802771	8.050727e-49	1.159149e-46
MsaG026054	MsaG031593	0.817523	5.492518e-52	1.132406e-49
MsaG026054	MsaG033125	0.813024	5.432804e-51	9.994187e-49
MsaG026054	MsaG034350	0.837409	9.698437e-57	3.493268e-54
MsaG026054	MsaG034529	0.802109	1.100440e-48	1.560575e-46
MsaG026054	MsaG035557	0.818623	3.105906e-52	6.588139e-50
MsaG026054	MsaG035582	0.912148	6.633166e-83	7.212997e-79
MsaG026054	MsaG035560	0.826555	4.530747e-54	1.189876e-51
MsaG026054	MsaG035563	0.806092	1.648164e-49	2.563321e-47
MsaG026054	MsaG035564	0.827352	2.928269e-54	7.863266e-52
MsaG026054	MsaG035567	0.828493	1.560830e-54	4.327937e-52
MsaG026054	MsaG035568	0.806656	1.255177e-49	1.978314e-47
MsaG026054	MsaG035572	0.825448	8.280499e-54	2.108758e-51
MsaG026054	MsaG036529	0.803080	6.956045e-49	1.008701e-46
MsaG026054	MsaG037763	0.828983	1.189368e-54	3.343441e-52
MsaG026054	MsaG039745	0.820155	1.395079e-52	3.081038e-50
MsaG026054	MsaG039830	0.815142	1.861079e-51	3.610489e-49
MsaG026054	MsaG040443	0.818487	3.333219e-52	7.045272e-50
MsaG026054	MsaG041765	0.832480	1.669450e-55	5.190557e-53
MsaG026054	MsaG041770	0.817339	6.040064e-52	1.239412e-49
MsaG026054	MsaG041772	0.805716	1.975229e-49	3.044898e-47
MsaG026054	MsaG043829	0.818350	3.579222e-52	7.538353e-50
MsaG026054	MsaG044807	0.813830	3.619226e-51	6.793863e-49
MsaG026054	MsaG044963	0.801712	1.326882e-48	1.864790e-46
MsaG026054	MsaG045283	0.802357	9.788814e-49	1.396181e-46
MsaG026054	MsaG045800	0.803497	5.706804e-49	8.355543e-47
MsaG026054	MsaG046156	0.808691	4.660813e-50	7.712308e-48
MsaG026054	MsaG046600	0.811523	1.150793e-50	2.039739e-48
MsaG026054	MsaG046616	0.846580	3.741816e-59	1.806622e-56
MsaG026054	MsaG046618	0.828146	1.890510e-54	5.191173e-52
MsaG026054	MsaG047020	0.804813	3.046695e-49	4.599010e-47
MsaG026054	MsaG004765	0.803194	6.589135e-49	9.580375e-47
MsaG026054	MsaG004834	0.806078	1.658726e-49	2.578924e-47
MsaG026054	MsaG002499	0.834752	4.550390e-56	1.513029e-53
MsaG026054	MsaG008748	0.800165	2.736914e-48	3.714222e-46
MsaG026054	MsaG013383	0.803244	6.433667e-49	9.365050e-47
MsaG026054	MsaG020544	0.803439	5.865015e-49	8.575807e-47
MsaG026054	MsaG028085	0.816583	8.913363e-52	1.793716e-49
MsaG026054	MsaG026617	0.845206	8.802425e-59	4.060651e-56
MsaG026054	MsaG026351	0.838781	4.316575e-57	1.622090e-54
MsaG026054	MsaG033538	0.818833	2.784835e-52	5.939456e-50
MsaG026054	MsaG032246	0.806035	1.693808e-49	2.630747e-47
MsaG026054	MsaG030191	0.800170	2.730439e-48	3.705847e-46
MsaG026054	MsaG031899	0.857883	2.349911e-62	1.687101e-59
MsaG026054	MsaG037043	0.834240	6.109910e-56	2.000521e-53
MsaG026054	MsaG037187	0.826616	4.382301e-54	1.152844e-51
MsaG026054	MsaG037387	0.809603	2.978008e-50	5.037091e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026054	MtrunA17_Chr5g0409471	90.090	333	24	1	1	333	1	324	0.0	586
MsaG026054	MtrunA17_Chr4g0021261	48.113	212	74	9	16	214	25	213	5.20e-49	167
MsaG026054	MtrunA17_Chr4g0070591	34.058	276	147	6	16	261	9	279	1.91e-43	153
MsaG026054	MtrunA17_Chr2g0300511	60.177	113	29	2	16	112	7	119	1.29e-40	145
MsaG026054	MtrunA17_Chr8g0343881	58.333	108	33	2	17	112	12	119	2.09e-39	142
MsaG026054	MtrunA17_Chr1g0152191	56.897	116	34	2	16	115	144	259	9.23e-37	139
MsaG026054	MtrunA17_Chr7g0265931	59.130	115	35	4	17	119	79	193	9.88e-36	133
MsaG026054	MtrunA17_Chr3g0127641	50.000	110	47	2	14	122	110	212	3.55e-27	111
MsaG026054	MtrunA17_Chr8g0351521	36.585	164	72	4	15	171	53	191	6.98e-20	89.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026054	AT3G13960.1	57.273	110	35	2	15	112	15	124	1.03e-38	142
MsaG026054	AT4G37740.1	40.580	207	96	6	16	203	164	362	1.05e-37	141
MsaG026054	AT3G52910.1	56.250	112	33	3	17	112	83	194	2.96e-33	127
MsaG026054	AT2G36400.1	54.783	115	37	3	17	116	77	191	3.63e-33	127
MsaG026054	AT2G22840.1	54.128	109	36	2	16	112	133	239	5.84e-31	122
MsaG026054	AT2G06200.2	50.400	125	39	3	9	110	38	162	1.66e-30	117
MsaG026054	AT2G06200.1	52.066	121	35	3	13	110	3	123	2.50e-30	115
MsaG026054	AT4G24150.3	46.610	118	47	2	17	118	112	229	3.96e-24	102
MsaG026054	AT4G24150.2	46.610	118	47	2	17	118	150	267	7.59e-24	102
MsaG026054	AT5G53660.1	49.485	97	44	1	16	112	59	150	1.35e-22	97.4
MsaG026054	AT4G24150.1	37.762	143	48	1	17	118	150	292	2.12e-21	95.1
MsaG026054	AT2G06200.3	72.917	48	11	1	65	110	4	51	6.22e-17	77.8
MsaG026054	AT2G45480.2	39.815	108	51	2	15	109	23	129	6.34e-17	80.5
MsaG026054	AT2G45480.1	39.815	108	51	2	15	109	21	127	2.05e-16	80.1
MsaG026054	AT2G45480.1	33.043	115	64	3	21	135	269	370	3.49e-13	70.5
MsaG026054	AT2G45480.3	39.815	108	51	2	15	109	23	129	2.09e-16	80.1
MsaG026054	AT2G45480.3	33.043	115	64	3	21	135	271	372	3.64e-13	70.5

Find 75 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCTATGCTTCTTCCTCTTC+AGG	0.174652	5:-16178612	MsaT026054.1:CDS
CTTCTACACCTACCTGGTTC+TGG	0.179105	5:+16178965	None:intergenic
TTTGTTTCAGTGGGTTATTA+TGG	0.331715	5:-16179001	MsaT026054.1:intron
TTCTACACCTACCTGGTTCT+GGG	0.343240	5:+16178966	None:intergenic
TTCCTAGATCCTTCTGTAAG+AGG	0.359958	5:+16176798	None:intergenic
GAAGGTTTAAGTTTCAATCT+TGG	0.362165	5:-16178656	MsaT026054.1:CDS
CCTATGCTTCTTCCTCTTCA+GGG	0.369011	5:-16178611	MsaT026054.1:CDS
AAGGTTTAAGTTTCAATCTT+GGG	0.373864	5:-16178655	MsaT026054.1:CDS
GATGTTCTAGCTCTTGCCAT+TGG	0.387285	5:+16179193	None:intergenic
TTTCAATCAACATTGACATC+TGG	0.399287	5:+16176452	None:intergenic
CCTCCTCTGCAATGGAGTAT+AGG	0.408064	5:-16176499	MsaT026054.1:CDS
TCCAAGGACATTGTCTTTAA+CGG	0.410437	5:-16176599	MsaT026054.1:CDS
ATGTTCTAGCTCTTGCCATT+GGG	0.417081	5:+16179194	None:intergenic
CTCTCTTCAGTGACTGGCAG+TGG	0.424103	5:-16176535	MsaT026054.1:CDS
GTTCTCTTGTTGTTCGCTGT+TGG	0.429758	5:+16176651	None:intergenic
AGGGTTCTAAGAAACCTTAT+AGG	0.432493	5:-16178592	MsaT026054.1:intron
CTCAGTCTCTCTTCAGTGAC+TGG	0.441726	5:-16176541	MsaT026054.1:CDS
CCAGCTAGGTAGTTTGAAAC+AGG	0.443147	5:-16176576	MsaT026054.1:CDS
CTTGGTGCTGGTGGTTCTAG+TGG	0.451681	5:-16178758	MsaT026054.1:CDS
ACACTTCCTTTGCATACAAA+TGG	0.453684	5:-16178686	MsaT026054.1:CDS
CGGTCACAGTACTTAGAATC+AGG	0.455848	5:+16178902	None:intergenic
GGTTATGATTTCACTTCAGA+TGG	0.470541	5:-16176761	MsaT026054.1:CDS
CAAACACTGTTTCAGAATCA+AGG	0.474671	5:-16176706	MsaT026054.1:CDS
ATCTTCAAGTATCTCAAAGC+TGG	0.478992	5:-16179162	MsaT026054.1:CDS
CGAATAGGTAAAAGGAGGTC+AGG	0.485852	5:+16179126	None:intergenic
GCCGTTAAAGACAATGTCCT+TGG	0.486308	5:+16176598	None:intergenic
TTGGTGTTAAAATCCTTGCT+TGG	0.489894	5:+16176476	None:intergenic
ATCAAGGAATTGTTCTTCCA+TGG	0.491166	5:-16176690	MsaT026054.1:CDS
ATCAACATTGACATCTGGAT+TGG	0.498766	5:+16176457	None:intergenic
AACCTCTTACAGAAGGATCT+AGG	0.500686	5:-16176800	MsaT026054.1:CDS
CTAGGAACTGTGAAGTATCA+AGG	0.513671	5:-16176782	MsaT026054.1:CDS
ATTCCACAGGCTTTCTTGAA+CGG	0.519412	5:+16178858	None:intergenic
CCCTGAAGAGGAAGAAGCAT+AGG	0.524973	5:+16178611	None:intergenic
CCTGTTTCAAACTACCTAGC+TGG	0.525039	5:+16176576	None:intergenic
GCATCGAGGTTAAACAGCTC+AGG	0.529759	5:+16176629	None:intergenic
ATTGTCTTTAACGGCCAGCT+AGG	0.531339	5:-16176590	MsaT026054.1:CDS
ACTTCACAGCAACTTGGTGC+TGG	0.533655	5:-16178770	MsaT026054.1:CDS
CATACAAATGGTACTCGTGA+AGG	0.534280	5:-16178674	MsaT026054.1:CDS
ATTTCACTTCAGATGGGATG+TGG	0.539855	5:-16176754	MsaT026054.1:CDS
TCTGTTCTTCTACACCTACC+TGG	0.541473	5:+16178959	None:intergenic
CGAGTACCATTTGTATGCAA+AGG	0.542113	5:+16178680	None:intergenic
TCACAGCAACTTGGTGCTGG+TGG	0.545124	5:-16178767	MsaT026054.1:CDS
TAATAGGTTTGGACTGAACT+CGG	0.548879	5:-16176837	MsaT026054.1:intron
CCTATACTCCATTGCAGAGG+AGG	0.551750	5:+16176499	None:intergenic
TGGGAACACCATGCCCCACA+TGG	0.552810	5:-16178636	MsaT026054.1:CDS
AGTACTACTTCACAGCAACT+TGG	0.553505	5:-16178776	MsaT026054.1:CDS
CATAGGGTCCATGTGGGGCA+TGG	0.558053	5:+16178628	None:intergenic
AGATTTAGCCTCCTCTGCAA+TGG	0.559132	5:-16176507	MsaT026054.1:CDS
TTCAACAACCGCCGTTGATG+TGG	0.565403	5:-16179245	MsaT026054.1:CDS
TGCCATTGGGAGACTGTGAA+CGG	0.571539	5:+16179207	None:intergenic
GTTATGATTTCACTTCAGAT+GGG	0.573421	5:-16176760	MsaT026054.1:CDS
GAAGAAGAAGAAGATTCCAC+AGG	0.592735	5:+16178845	None:intergenic
CCTCGATGCTGCAAGATCCA+AGG	0.599199	5:-16176615	MsaT026054.1:CDS
TGGACTGAACTCGGAAGCAG+AGG	0.606816	5:-16176828	MsaT026054.1:CDS
CACCGTTCACAGTCTCCCAA+TGG	0.611493	5:-16179209	MsaT026054.1:CDS
GGAGAACAACCTCTTACAGA+AGG	0.617644	5:-16176807	MsaT026054.1:CDS
ACTGTGAACGGTGGATGAGT+TGG	0.621139	5:+16179219	None:intergenic
GGTAGGTGTAGAAGAACAGA+TGG	0.627953	5:-16178956	MsaT026054.1:CDS
GGTTTCTTAGAACCCTGAAG+AGG	0.629209	5:+16178599	None:intergenic
CCTGAAGAGGAAGAAGCATA+GGG	0.630158	5:+16178612	None:intergenic
GTCACTGAAGAGAGACTGAG+AGG	0.630803	5:+16176544	None:intergenic
AAGAAGCATAGGGTCCATGT+GGG	0.631241	5:+16178622	None:intergenic
GAAGAAGCATAGGGTCCATG+TGG	0.633928	5:+16178621	None:intergenic
TGAGTTGGTGACCACATCAA+CGG	0.634344	5:+16179234	None:intergenic
GAAGTTGTAGTTGTCAACCA+TGG	0.647831	5:+16176673	None:intergenic
TTACCGTTCAAGAAAGCCTG+TGG	0.651222	5:-16178861	MsaT026054.1:CDS
AATGGAGTATAGGCCAAGCA+AGG	0.658290	5:-16176489	MsaT026054.1:CDS
AAATTGACCCAGAACCAGGT+AGG	0.671324	5:-16178973	MsaT026054.1:CDS
TGGCCTATACTCCATTGCAG+AGG	0.676725	5:+16176496	None:intergenic
CCTTGGATCTTGCAGCATCG+AGG	0.684738	5:+16176615	None:intergenic
ATAGGTAAAAGGAGGTCAGG+AGG	0.695933	5:+16179129	None:intergenic
AACGACGTCGAATCATATGA+CGG	0.698475	5:+16178882	None:intergenic
AGAAGCATAGGGTCCATGTG+GGG	0.720767	5:+16178623	None:intergenic
CATTGGGAGACTGTGAACGG+TGG	0.744499	5:+16179210	None:intergenic
GTTGGTGACCACATCAACGG+CGG	0.749963	5:+16179237	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	16176454	16179274	16176454	ID=MsaG026054
Chr5	mRNA	16176454	16179274	16176454	ID=MsaT026054.1;Parent=MsaG026054
Chr5	exon	16176454	16176853	16176454	ID=MsaT026054.1.exon3;Parent=MsaT026054.1
Chr5	CDS	16176454	16176853	16176454	ID=cds.MsaT026054.1;Parent=MsaT026054.1
Chr5	exon	16178593	16179013	16178593	ID=MsaT026054.1.exon2;Parent=MsaT026054.1
Chr5	CDS	16178593	16179013	16178593	ID=cds.MsaT026054.1;Parent=MsaT026054.1
Chr5	exon	16179094	16179274	16179094	ID=MsaT026054.1.exon1;Parent=MsaT026054.1
Chr5	CDS	16179094	16179274	16179094	ID=cds.MsaT026054.1;Parent=MsaT026054.1

Gene Sequence

>MsaT026054.1

ATGAGCATTCAACAACCGCCGTTGATGTGGTCACCAACTCATCCACCGTTCACAGTCTCCCAATGGCAAGAGCTAGAACATCAAGCTCTCATCTTCAAGTATCTCAAAGCTGGTTTATCTGTTCCTCCTGACCTCCTTTTACCTATTCGGAAAAGTCTTCAATTCATGTCTCATCCATCTCTGGGTTATTATGGAAAAAAAATTGACCCAGAACCAGGTAGGTGTAGAAGAACAGATGGCAAAAAATGGAGATGTGCTAGGGATGCACATCCTGATTCTAAGTACTGTGACCGTCATATGATTCGACGTCGTTACCGTTCAAGAAAGCCTGTGGAATCTTCTTCTTCTTCTTCTTCTTGTTCACAATCTTCATCTTCAGTTTCAGTTTCTACTACTAGTACTACTTCACAGCAACTTGGTGCTGGTGGTTCTAGTGGAAGTACTAGTACTGTTTCTGCTTCTGTTACTGCTGCTTCAACTTTTCATACACTTCCTTTGCATACAAATGGTACTCGTGAAGGTTTAAGTTTCAATCTTGGGAACACCATGCCCCACATGGACCCTATGCTTCTTCCTCTTCAGGGTTCTAAGAAACCTTATAGGTTTGGACTGAACTCGGAAGCAGAGGAGAACAACCTCTTACAGAAGGATCTAGGAACTGTGAAGTATCAAGGTTATGATTTCACTTCAGATGGGATGTGGTATAATAACATGTCTCAGATTTCATCAAACACTGTTTCAGAATCAAGGAATTGTTCTTCCATGGTTGACAACTACAACTTCCAACAGCGAACAACAAGAGAACCTGAGCTGTTTAACCTCGATGCTGCAAGATCCAAGGACATTGTCTTTAACGGCCAGCTAGGTAGTTTGAAACAGGAATATCAATCCTCTCAGTCTCTCTTCAGTGACTGGCAGTGGAAGAAAGATTTAGCCTCCTCTGCAATGGAGTATAGGCCAAGCAAGGATTTTAACACCAATCCAGATGTCAATGTTGATTGA

Protein sequence

>MsaT026054.1

MSIQQPPLMWSPTHPPFTVSQWQELEHQALIFKYLKAGLSVPPDLLLPIRKSLQFMSHPSLGYYGKKIDPEPGRCRRTDGKKWRCARDAHPDSKYCDRHMIRRRYRSRKPVESSSSSSSCSQSSSSVSVSTTSTTSQQLGAGGSSGSTSTVSASVTAASTFHTLPLHTNGTREGLSFNLGNTMPHMDPMLLPLQGSKKPYRFGLNSEAEENNLLQKDLGTVKYQGYDFTSDGMWYNNMSQISSNTVSESRNCSSMVDNYNFQQRTTREPELFNLDAARSKDIVFNGQLGSLKQEYQSSQSLFSDWQWKKDLASSAMEYRPSKDFNTNPDVNVD*