Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026136 | XP_004502721.1 | 91.489 | 47 | 4 | 0 | 6 | 52 | 178 | 224 | 7.80e-20 | 91.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026136 | sp|Q9LJL9|CAMK2_ARATH | 76.596 | 47 | 11 | 0 | 6 | 52 | 180 | 226 | 1.71e-18 | 79.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026136 | tr|A0A1S2YDS2|A0A1S2YDS2_CICAR | 91.489 | 47 | 4 | 0 | 6 | 52 | 178 | 224 | 3.73e-20 | 91.7 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026136 | MtrunA17_Chr3g0127571 | 91.489 | 47 | 4 | 0 | 6 | 52 | 174 | 220 | 1.15e-23 | 91.3 |
| MsaG026136 | MtrunA17_Chr4g0045261 | 76.596 | 47 | 11 | 0 | 6 | 52 | 173 | 219 | 2.99e-19 | 78.6 |
| MsaG026136 | MtrunA17_Chr5g0402551 | 74.468 | 47 | 12 | 0 | 6 | 52 | 170 | 216 | 3.14e-19 | 78.6 |
| MsaG026136 | MtrunA17_Chr7g0276111 | 74.468 | 47 | 12 | 0 | 6 | 52 | 180 | 226 | 4.25e-19 | 78.2 |
| MsaG026136 | MtrunA17_Chr8g0374701 | 68.085 | 47 | 15 | 0 | 6 | 52 | 164 | 210 | 4.12e-18 | 75.5 |
| MsaG026136 | MtrunA17_Chr7g0256001 | 65.957 | 47 | 16 | 0 | 6 | 52 | 177 | 223 | 8.73e-18 | 74.7 |
| MsaG026136 | MtrunA17_Chr5g0405421 | 57.447 | 47 | 20 | 0 | 6 | 52 | 132 | 178 | 1.14e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026136 | AT3G19100.1 | 76.596 | 47 | 11 | 0 | 6 | 52 | 180 | 226 | 1.73e-19 | 79.7 |
| MsaG026136 | AT3G56760.1 | 72.340 | 47 | 13 | 0 | 6 | 52 | 160 | 206 | 7.62e-19 | 77.8 |
| MsaG026136 | AT2G41140.1 | 72.340 | 47 | 13 | 0 | 6 | 52 | 159 | 205 | 1.49e-18 | 77.0 |
| MsaG026136 | AT5G24430.1 | 68.085 | 47 | 15 | 0 | 6 | 52 | 179 | 225 | 2.04e-18 | 76.6 |
| MsaG026136 | AT1G49580.1 | 72.340 | 47 | 13 | 0 | 6 | 52 | 186 | 232 | 2.34e-18 | 76.6 |
| MsaG026136 | AT2G46700.1 | 72.340 | 47 | 13 | 0 | 6 | 52 | 179 | 225 | 3.81e-18 | 75.9 |
| MsaG026136 | AT3G49370.1 | 68.085 | 47 | 15 | 0 | 6 | 52 | 178 | 224 | 4.96e-18 | 75.5 |
| MsaG026136 | AT3G50530.1 | 72.340 | 47 | 13 | 0 | 6 | 52 | 184 | 230 | 7.92e-18 | 75.1 |
| MsaG026136 | AT3G50530.2 | 72.340 | 47 | 13 | 0 | 6 | 52 | 184 | 230 | 7.93e-18 | 75.1 |
| MsaG026136 | AT2G46700.2 | 75.000 | 44 | 11 | 0 | 9 | 52 | 1 | 44 | 6.26e-17 | 72.4 |
| MsaG026136 | AT2G46700.3 | 75.000 | 44 | 11 | 0 | 9 | 52 | 1 | 44 | 6.26e-17 | 72.4 |
| MsaG026136 | AT2G17890.1 | 63.830 | 47 | 17 | 0 | 6 | 52 | 141 | 187 | 1.41e-15 | 68.6 |
| MsaG026136 | AT4G36070.1 | 57.447 | 47 | 20 | 0 | 6 | 52 | 104 | 150 | 1.04e-12 | 60.5 |
| MsaG026136 | AT4G36070.2 | 57.447 | 47 | 20 | 0 | 6 | 52 | 104 | 150 | 1.04e-12 | 60.5 |
| MsaG026136 | AT5G66210.6 | 55.319 | 47 | 21 | 0 | 6 | 52 | 95 | 141 | 9.95e-12 | 57.8 |
| MsaG026136 | AT5G66210.4 | 55.319 | 47 | 21 | 0 | 6 | 52 | 95 | 141 | 9.95e-12 | 57.8 |
| MsaG026136 | AT5G66210.1 | 55.319 | 47 | 21 | 0 | 6 | 52 | 95 | 141 | 1.07e-11 | 57.8 |
| MsaG026136 | AT5G66210.2 | 55.319 | 47 | 21 | 0 | 6 | 52 | 95 | 141 | 1.07e-11 | 57.8 |
| MsaG026136 | AT5G66210.3 | 55.319 | 47 | 21 | 0 | 6 | 52 | 95 | 141 | 1.16e-11 | 57.4 |
| MsaG026136 | AT5G66210.5 | 55.319 | 47 | 21 | 0 | 6 | 52 | 95 | 141 | 1.17e-11 | 57.4 |
Find 14 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTATCTGGTCATAGGAATT+TGG | 0.265283 | 5:-17289730 | MsaT026136.1:CDS |
| AAAATGTTGAAGGCCTTATC+TGG | 0.375299 | 5:-17289744 | MsaT026136.1:CDS |
| ATTGAAGATGTGAGGAGAGA+GGG | 0.443048 | 5:-17289768 | MsaT026136.1:CDS |
| TGAAGGCCTTATCTGGTCAT+AGG | 0.469096 | 5:-17289737 | MsaT026136.1:CDS |
| CAAATTCCTATGACCAGATA+AGG | 0.477339 | 5:+17289731 | None:intergenic |
| TTGAAGATGTGAGGAGAGAG+GGG | 0.515894 | 5:-17289767 | MsaT026136.1:CDS |
| CGCTGAGAACGGAATATGGC+CGG | 0.531363 | 5:-17290356 | None:intergenic |
| GAGAGAGGGGAAAATGTTGA+AGG | 0.555863 | 5:-17289754 | MsaT026136.1:CDS |
| AATTGAAGATGTGAGGAGAG+AGG | 0.561921 | 5:-17289769 | MsaT026136.1:CDS |
| CACCTCTGCTCTACCGTCAC+CGG | 0.565843 | 5:+17290337 | None:intergenic |
| GAACGGAATATGGCCGGTGA+CGG | 0.573883 | 5:-17290350 | None:intergenic |
| GGCCGGTGACGGTAGAGCAG+AGG | 0.618426 | 5:-17290339 | MsaT026136.1:intron |
| GAATAACGTCTACATTGTGA+TGG | 0.632991 | 5:-17289679 | MsaT026136.1:CDS |
| CAATAACAATTGAAGATGTG+AGG | 0.635983 | 5:-17289776 | MsaT026136.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 17289668 | 17290363 | 17289668 | ID=MsaG026136 |
| Chr5 | mRNA | 17289668 | 17290363 | 17289668 | ID=MsaT026136.1;Parent=MsaG026136 |
| Chr5 | exon | 17289668 | 17289808 | 17289668 | ID=MsaT026136.1.exon2;Parent=MsaT026136.1 |
| Chr5 | CDS | 17289668 | 17289808 | 17289668 | ID=cds.MsaT026136.1;Parent=MsaT026136.1 |
| Chr5 | exon | 17290340 | 17290363 | 17290340 | ID=MsaT026136.1.exon1;Parent=MsaT026136.1 |
| Chr5 | CDS | 17290340 | 17290363 | 17290340 | ID=cds.MsaT026136.1;Parent=MsaT026136.1 |
| Gene Sequence |
| Protein sequence |
