AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG026146

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026146 MtrunA17_Chr5g0410711 98.254 802 13 1 1 802 1 801 0.0 1642
MsaG026146 MtrunA17_Chr6g0481041 47.613 733 360 13 61 779 1 723 0.0 661
MsaG026146 MtrunA17_Chr2g0322731 44.429 736 360 15 57 781 70 767 0.0 620
MsaG026146 MtrunA17_Chr8g0350321 44.027 745 386 16 49 779 79 806 0.0 615
MsaG026146 MtrunA17_Chr4g0024351 46.023 704 337 13 89 781 60 731 0.0 615
MsaG026146 MtrunA17_Chr7g0246631 44.307 729 367 16 66 779 70 774 0.0 604
MsaG026146 MtrunA17_Chr1g0203671 41.272 739 406 13 57 779 63 789 0.0 571
MsaG026146 MtrunA17_Chr6g0472471 41.791 737 396 17 63 779 42 765 1.70e-176 526
MsaG026146 MtrunA17_Chr2g0331581 38.205 691 379 10 93 779 143 789 1.65e-160 486
MsaG026146 MtrunA17_Chr7g0224451 41.529 484 215 9 89 565 16 438 1.85e-119 367
MsaG026146 MtrunA17_Chr5g0394111 36.346 509 295 12 282 779 156 646 2.64e-99 322
MsaG026146 MtrunA17_Chr5g0394111 45.390 141 72 3 71 211 18 153 4.09e-27 117
MsaG026146 MtrunA17_Chr2g0292731 34.369 515 297 14 282 779 58 548 2.71e-90 295
MsaG026146 MtrunA17_Chr2g0292741 33.528 513 299 15 282 779 50 535 6.23e-85 280
MsaG026146 MtrunA17_Chr7g0224481 40.789 380 163 7 89 462 16 339 7.03e-82 265
MsaG026146 MtrunA17_Chr7g0224491 42.902 317 155 8 465 779 1 293 1.49e-72 239
MsaG026146 MtrunA17_Chr2g0292761 33.090 547 290 21 170 705 3 484 3.91e-71 243
MsaG026146 MtrunA17_Chr5g0419821 50.806 124 59 2 88 210 32 154 8.92e-34 127
MsaG026146 MtrunA17_Chr2g0319671 32.710 214 127 5 341 553 104 301 1.60e-26 110
MsaG026146 MtrunA17_Chr2g0304191 34.307 137 78 3 295 425 1 131 1.50e-20 88.6
MsaG026146 MtrunA17_Chr2g0319691 39.024 123 71 2 401 523 1 119 3.01e-20 88.2
MsaG026146 MtrunA17_Chr2g0319681 27.615 239 155 5 545 779 1 225 7.40e-19 87.0
MsaG026146 MtrunA17_Chr7g0224431 33.129 163 66 3 310 472 1 120 6.88e-18 80.5
MsaG026146 MtrunA17_Chr1g0205981 34.359 195 96 8 144 329 1 172 1.49e-17 82.0
MsaG026146 MtrunA17_Chr2g0319701 28.151 238 153 8 545 779 1 223 3.12e-15 76.3
MsaG026146 MtrunA17_Chr7g0224461 38.333 120 67 2 660 779 2 114 5.00e-14 69.3
MsaG026146 MtrunA17_Chr4g0055851 35.115 131 84 1 48 177 75 205 7.48e-14 72.8
MsaG026146 MtrunA17_Chr7g0265521 36.937 111 64 2 77 184 51 158 1.94e-11 64.7
MsaG026146 MtrunA17_Chr4g0028991 34.694 98 56 2 92 181 28 125 2.31e-11 67.8
MsaG026146 MtrunA17_Chr7g0258831 31.250 128 85 1 64 188 129 256 5.84e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026146 AT5G46880.3 55.355 831 287 21 8 779 1 806 0.0 842
MsaG026146 AT5G46880.1 55.355 831 287 21 8 779 1 806 0.0 842
MsaG026146 AT5G46880.2 55.355 831 287 21 8 779 1 806 0.0 842
MsaG026146 AT4G17710.1 48.582 776 308 13 13 779 10 703 0.0 663
MsaG026146 AT4G04890.2 44.709 756 381 13 34 779 7 735 0.0 649
MsaG026146 AT4G04890.1 44.709 756 381 13 34 779 7 735 0.0 649
MsaG026146 AT1G05230.8 44.640 737 351 16 51 779 29 716 0.0 629
MsaG026146 AT1G05230.4 44.640 737 351 16 51 779 29 716 0.0 629
MsaG026146 AT1G05230.9 44.640 737 351 16 51 779 29 716 0.0 629
MsaG026146 AT1G05230.10 44.640 737 351 16 51 779 29 716 0.0 629
MsaG026146 AT1G05230.7 44.640 737 351 16 51 779 29 716 0.0 629
MsaG026146 AT1G05230.11 44.640 737 351 16 51 779 29 716 0.0 629
MsaG026146 AT1G05230.2 44.640 737 351 16 51 779 29 716 0.0 629
MsaG026146 AT1G05230.6 44.640 737 351 16 51 779 29 716 0.0 629
MsaG026146 AT1G05230.5 44.640 737 351 16 51 779 29 716 0.0 629
MsaG026146 AT1G05230.1 44.640 737 351 16 51 779 29 716 0.0 629
MsaG026146 AT1G05230.3 44.369 737 351 16 51 779 29 714 0.0 624
MsaG026146 AT4G21750.2 43.586 764 374 15 55 782 19 761 0.0 620
MsaG026146 AT4G21750.1 43.586 764 374 15 55 782 19 761 0.0 620
MsaG026146 AT4G21750.3 43.586 764 374 15 55 782 19 761 0.0 620
MsaG026146 AT4G21750.4 43.586 764 374 15 55 782 19 761 0.0 620
MsaG026146 AT1G73360.1 46.348 712 343 15 89 785 32 719 0.0 600
MsaG026146 AT4G00730.1 42.527 736 372 16 55 779 103 798 0.0 576
MsaG026146 AT1G17920.2 44.537 723 335 17 75 779 7 681 0.0 575
MsaG026146 AT1G17920.1 44.537 723 335 17 75 779 7 681 0.0 575
MsaG026146 AT3G61150.1 40.726 744 389 16 63 779 85 803 0.0 557
MsaG026146 AT1G05230.12 46.305 609 287 13 51 651 29 605 0.0 544
MsaG026146 AT2G32370.1 38.149 713 383 15 75 776 52 717 1.15e-156 474
MsaG026146 AT5G52170.3 38.986 690 346 18 93 779 61 678 9.30e-156 470
MsaG026146 AT5G52170.1 38.986 690 346 18 93 779 61 678 9.30e-156 470
MsaG026146 AT5G52170.2 38.728 692 350 18 93 779 61 683 1.91e-155 469
MsaG026146 AT1G79840.3 38.265 703 361 17 89 779 43 684 3.40e-150 456
MsaG026146 AT1G79840.6 38.265 703 361 17 89 779 43 684 3.40e-150 456
MsaG026146 AT1G79840.1 38.265 703 361 17 89 779 101 742 9.90e-150 457
MsaG026146 AT1G79840.2 38.265 703 361 17 89 779 130 771 7.62e-149 455
MsaG026146 AT3G03260.1 35.628 741 408 19 61 779 1 694 2.17e-137 423
MsaG026146 AT4G00730.2 45.607 478 225 10 55 524 103 553 1.31e-136 417
MsaG026146 AT5G17320.1 34.097 742 423 18 72 779 6 715 2.21e-126 395
MsaG026146 AT1G79840.4 38.624 567 291 13 89 645 101 620 1.79e-123 385
MsaG026146 AT1G34650.1 34.384 730 409 24 84 779 12 705 6.04e-115 365
MsaG026146 AT4G25530.1 31.215 708 417 13 83 779 34 682 4.19e-112 357
MsaG026146 AT3G03260.2 37.037 567 320 14 61 618 1 539 4.52e-111 349
MsaG026146 AT1G79840.5 40.045 447 218 11 82 523 94 495 4.58e-94 304
MsaG026146 AT5G07260.1 25.295 593 352 19 154 736 9 520 8.03e-38 149
MsaG026146 AT5G07260.2 25.126 593 348 20 154 736 9 515 3.12e-37 147
MsaG026146 AT4G26920.1 25.431 232 152 10 297 523 74 289 1.24e-11 68.2
MsaG026146 AT5G60690.1 35.714 98 55 2 92 181 25 122 1.81e-11 68.6

Find 184 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATACGCTTCCATTTCT+TGG 0.178284 5:-17420362 None:intergenic
AGAGGACCTCCTGCAGTATT+TGG 0.191744 5:-17423666 None:intergenic
TTCAGTCTTTGTTTATCATC+TGG 0.234084 5:-17420711 None:intergenic
ATGTATAATGGGACCTGATA+TGG 0.242844 5:+17421576 MsaT026146.1:CDS
AGAGGGTGGTCTCTTATTAA+TGG 0.264546 5:+17422695 MsaT026146.1:CDS
CAGATGGATGCACTTTCTAA+TGG 0.294055 5:+17424514 MsaT026146.1:intron
CATAGTTACTAAATATTTGA+TGG 0.294552 5:-17423863 None:intergenic
ATCAAGCTCTTTCGCGCTTC+CGG 0.296499 5:-17424082 None:intergenic
GGAACTTGCAGCTTCTTCTA+TGG 0.300980 5:+17422740 MsaT026146.1:CDS
ATCATAGCTTCAGGTGCTTC+TGG 0.303903 5:+17423136 MsaT026146.1:CDS
CACCACAAGTGGTCATATTA+AGG 0.320117 5:-17424138 None:intergenic
ACTGAAACTAAGCCATGATT+TGG 0.329620 5:+17420728 MsaT026146.1:CDS
GCTTGATGAAATTAGCAAAT+AGG 0.352169 5:+17424098 MsaT026146.1:CDS
TTGTGGTGGGCCATGTATAA+TGG 0.362098 5:+17421564 MsaT026146.1:CDS
CTCAGGTTGTCTTCTTATCA+TGG 0.367340 5:+17424960 MsaT026146.1:CDS
ATCATGGATCAAAGTGGTTC+AGG 0.367987 5:+17420237 MsaT026146.1:CDS
CTTGCTCTAGCAACAATTGT+TGG 0.385997 5:-17423103 None:intergenic
ATACATGGCCCACCACAATT+AGG 0.390584 5:-17421559 None:intergenic
TCAGTCTTTGTTTATCATCT+GGG 0.391749 5:-17420710 None:intergenic
ACTAACAGCAATTGCTTTGT+TGG 0.392291 5:+17425102 MsaT026146.1:CDS
AGATGGATGCACTTTCTAAT+GGG 0.399022 5:+17424515 MsaT026146.1:intron
CTCTTTCGCGCTTCCGGAGA+AGG 0.400542 5:-17424076 None:intergenic
ATGGTGGTGGTGCCATTGTT+TGG 0.412301 5:-17422551 None:intergenic
GTGATTCTAACGGTGTCTTC+TGG 0.415111 5:-17424190 None:intergenic
AATTATATGCAGTTTGTTCA+AGG 0.423365 5:+17420677 MsaT026146.1:intron
ATCACTCCACTGGGTTGGCC+AGG 0.423809 5:-17424232 None:intergenic
TGAAGTTGAAGTTAGAGTTA+TGG 0.424415 5:-17420063 None:intergenic
GAGGACCTCCTGCAGTATTT+GGG 0.424936 5:-17423665 None:intergenic
GACAAGGAGTGCACACTACT+TGG 0.425308 5:-17425157 None:intergenic
ACAATCATGGACTGCTATAT+CGG 0.426472 5:+17424162 MsaT026146.1:CDS
CTAACAGCAATTGCTTTGTT+GGG 0.426498 5:+17425103 MsaT026146.1:CDS
ATCTCTTATGCCACCATCAC+TGG 0.427634 5:+17422572 MsaT026146.1:CDS
TATTGCTAACGGTTCACATC+CGG 0.428988 5:+17424561 MsaT026146.1:CDS
CAAATTGAAGGTGCTCTTTG+TGG 0.429451 5:+17425075 MsaT026146.1:CDS
TGTACCCTTGAATAGCCATT+TGG 0.431448 5:-17423710 None:intergenic
TTTGAACAGAAAGAAGAAGA+AGG 0.433841 5:+17420192 MsaT026146.1:intron
TTACTAAATATTTGATGGAC+TGG 0.435592 5:-17423858 None:intergenic
TTTGGTGCACAACGTTGGTT+AGG 0.436385 5:+17423903 MsaT026146.1:CDS
AGAAGGGAAATGCAGTTTCC+CGG 0.438847 5:-17424580 None:intergenic
AAGACTTTGGTATGAGGATA+AGG 0.441439 5:-17424283 None:intergenic
AGAGATTAATAATGAACAAC+AGG 0.441688 5:+17420296 MsaT026146.1:CDS
AACTTACATTTATGCGAAGA+AGG 0.441914 5:-17424597 None:intergenic
TGATCATCAAAGCCCATATC+AGG 0.443548 5:-17421589 None:intergenic
TGCTCGTCAGATCCAAGAAA+TGG 0.444102 5:+17420350 MsaT026146.1:CDS
GATTGCAGTGATACCTTCTC+CGG 0.445102 5:+17424063 MsaT026146.1:intron
TTTAGTAACTATGTGTATAG+TGG 0.445206 5:+17423873 MsaT026146.1:CDS
GAAGAAGGAAAAGGTTGAAA+AGG 0.449348 5:-17420097 None:intergenic
GTTTGAAGAATGAGAACTAT+AGG 0.449425 5:+17421506 MsaT026146.1:CDS
TTAAGCAAAGGAATGAGTTA+AGG 0.450292 5:+17422880 MsaT026146.1:CDS
GCTAGAAACATATCAGATCT+TGG 0.453635 5:+17423966 MsaT026146.1:CDS
ATTCCAAGTTCTTTCTCCAT+TGG 0.455604 5:+17423526 MsaT026146.1:CDS
ATTTCCGTGCATGGTGGCAT+TGG 0.456028 5:-17422630 None:intergenic
CCGAAGAAAATGAGTCTCTC+TGG 0.461082 5:-17423555 None:intergenic
TTCCTTTGCTTAAGATTCAA+AGG 0.462942 5:-17422870 None:intergenic
GTCAAATGAACGAACCACTT+TGG 0.464244 5:+17422781 MsaT026146.1:CDS
AAATACTGCAGGAGGTCCTC+TGG 0.464331 5:+17423668 MsaT026146.1:CDS
ACTGTTCAAATCATAGCTTC+AGG 0.465215 5:+17423127 MsaT026146.1:CDS
TGTGGTGGGCCATGTATAAT+GGG 0.465808 5:+17421565 MsaT026146.1:CDS
CTGAAACTAAGCCATGATTT+GGG 0.466763 5:+17420729 MsaT026146.1:CDS
GAACAAGTTGAAGATAAGTC+AGG 0.474547 5:+17420264 MsaT026146.1:CDS
AACCACGCCAGGTTAAGTTC+TGG 0.478239 5:+17420757 MsaT026146.1:CDS
TCTTGAAAATGTGATGGTTC+AGG 0.479375 5:-17422675 None:intergenic
GTGGTTTCAGTCCCAAATCA+TGG 0.480470 5:-17420740 None:intergenic
TGACGAACGCCACAGATCTC+AGG 0.483891 5:+17424318 MsaT026146.1:CDS
GAGAGGTAAAGAAGAGATCA+TGG 0.485278 5:+17420221 MsaT026146.1:CDS
TTCGTAGCGGAGAGAATGAT+AGG 0.486066 5:+17422805 MsaT026146.1:CDS
AATGGCCCAGGTTCCTTCCT+CGG 0.487016 5:-17423594 None:intergenic
GAGCACCTTCAATTTGATGC+AGG 0.487171 5:-17425068 None:intergenic
AATTATGTATGCAGGTAACA+TGG 0.487587 5:+17423808 MsaT026146.1:intron
TGTATAATGGGACCTGATAT+GGG 0.487914 5:+17421577 MsaT026146.1:CDS
CCACTTGTGGTGAACAATCA+TGG 0.492598 5:+17424149 MsaT026146.1:CDS
ACATGCCAAATGGCTATTCA+AGG 0.495938 5:+17423705 MsaT026146.1:CDS
CTTTGTTTATCATCTGGGTG+TGG 0.497200 5:-17420705 None:intergenic
ATACCAATGGAGAAAGAACT+TGG 0.497986 5:-17423529 None:intergenic
GAGAGTTGCACAGATCAATC+TGG 0.500290 5:+17424745 MsaT026146.1:CDS
TTGGAGGGTGCACTAGTGGT+TGG 0.501171 5:-17425138 None:intergenic
AATACCCTGCATCAAATTGA+AGG 0.504038 5:+17425063 MsaT026146.1:CDS
TCGCCGCAGTTTCAACCACT+TGG 0.506037 5:+17424257 MsaT026146.1:CDS
GTGTATAGTGGAATGGCATT+TGG 0.507399 5:+17423885 MsaT026146.1:CDS
CACGACAGTCGATGTTGATT+CGG 0.508112 5:+17424783 MsaT026146.1:CDS
CGATCCAATGCCACCATGCA+CGG 0.512769 5:+17422626 MsaT026146.1:CDS
TGCGGCGAGAATCACTCCAC+TGG 0.516058 5:-17424242 None:intergenic
CTTGTCCACTCATAGCTAGT+TGG 0.519744 5:-17424807 None:intergenic
GGTGCTTCTGGCCATGCAAG+TGG 0.520606 5:+17423148 MsaT026146.1:CDS
ATTGCTAACGGTTCACATCC+GGG 0.521364 5:+17424562 MsaT026146.1:CDS
TGTCAAGTAATGTCAACAAT+GGG 0.522321 5:+17419995 MsaT026146.1:CDS
ACAACCTGAGTTAGCAGCAT+TGG 0.530947 5:-17424947 None:intergenic
TGCAAGTGGGACTCTTCAGC+TGG 0.531576 5:+17423162 MsaT026146.1:CDS
GATGGTTCAGGTGGTAACAT+AGG 0.532769 5:-17422663 None:intergenic
TCAGGTTGTCTTCTTATCAT+GGG 0.533492 5:+17424961 MsaT026146.1:CDS
ACTAGGAAAATCACTGAGCC+TGG 0.535391 5:+17424214 MsaT026146.1:CDS
AAAATGCCGAGGAAGGAACC+TGG 0.537052 5:+17423588 MsaT026146.1:CDS
CTACTTGGAGGGTGCACTAG+TGG 0.538012 5:-17425142 None:intergenic
AACTTCGATTTGAAAATGCC+CGG 0.538277 5:+17421614 MsaT026146.1:CDS
AGGAAAATCAACAATGGCCC+AGG 0.539512 5:-17423606 None:intergenic
ACAGGAATCATTTCCGTGCA+TGG 0.540403 5:-17422639 None:intergenic
AGATCAAAGACTTTGGTATG+AGG 0.540751 5:-17424289 None:intergenic
GGCCTTTGAATCTTAAGCAA+AGG 0.541375 5:+17422868 MsaT026146.1:CDS
TCAGGTGGTAACATAGGGAC+AGG 0.544013 5:-17422657 None:intergenic
TCATCTGAACAAGGTGTCAC+AGG 0.545128 5:+17424901 MsaT026146.1:CDS
ATTCTTGTCCCAAATACTGC+AGG 0.549607 5:+17423657 MsaT026146.1:CDS
AAGGAGTGCACACTACTTGG+AGG 0.551266 5:-17425154 None:intergenic
ACTTACATTTATGCGAAGAA+GGG 0.551867 5:-17424596 None:intergenic
TGGGAACTCATTAAATGAAG+TGG 0.552199 5:+17424534 MsaT026146.1:CDS
ATGGTTCAGGTGGTAACATA+GGG 0.552742 5:-17422662 None:intergenic
TTTGGGACTGAAACCACGCC+AGG 0.553427 5:+17420746 MsaT026146.1:CDS
TGAGAACTATAGGCTACAAT+CGG 0.553444 5:+17421516 MsaT026146.1:CDS
TAACTATGTGTATAGTGGAA+TGG 0.553607 5:+17423878 MsaT026146.1:CDS
AGCCTTAATATGACCACTTG+TGG 0.554303 5:+17424136 MsaT026146.1:CDS
GGCATTGGATCGAAGTGCCT+CGG 0.554413 5:-17422615 None:intergenic
TTTCCTAGTTGTGATTCTAA+CGG 0.554507 5:-17424200 None:intergenic
ATTACCATGGAAGAAGGAAA+AGG 0.555298 5:-17420106 None:intergenic
ACGAAGGAGATCAAAGACTT+TGG 0.555409 5:-17424296 None:intergenic
GAAGAAGAAGGAATATTGAG+AGG 0.555846 5:+17420204 MsaT026146.1:CDS
TTGAAGTTAGAGTTATGGAT+CGG 0.556544 5:-17420058 None:intergenic
TTTGCAGAGGCAATGTGAAA+GGG 0.557444 5:+17423929 MsaT026146.1:CDS
AGCACCTTCAATTTGATGCA+GGG 0.561062 5:-17425067 None:intergenic
TCAGCATTTGATGAAACCAT+TGG 0.561093 5:-17424874 None:intergenic
TGCATTGCACTTCTTCCACA+AGG 0.562490 5:+17424838 MsaT026146.1:CDS
CCATGATTGTTCACCACAAG+TGG 0.563738 5:-17424149 None:intergenic
AGGGTTCATGATAGTGCCAA+TGG 0.566530 5:+17424858 MsaT026146.1:CDS
GTGCTTCTGGCCATGCAAGT+GGG 0.568255 5:+17423149 MsaT026146.1:CDS
GAAAGGTTAAGCTTTGCAGA+AGG 0.572294 5:-17425009 None:intergenic
CAAGTAATGTCAACAATGGG+AGG 0.574313 5:+17419998 MsaT026146.1:CDS
AGTGCCAATGCTGCTAACTC+AGG 0.574318 5:+17424943 MsaT026146.1:CDS
CGAGAATCACTCCACTGGGT+TGG 0.576021 5:-17424237 None:intergenic
AACCAGAACTTAACCTGGCG+TGG 0.579376 5:-17420759 None:intergenic
GTCTCTCTGGTGGATACCAA+TGG 0.579467 5:-17423542 None:intergenic
GAAGTGGCTCATATTGCTAA+CGG 0.583975 5:+17424550 MsaT026146.1:CDS
GCTGAAGAGTCCCACTTGCA+TGG 0.585312 5:-17423159 None:intergenic
GAATCGTCGAACTCAGATGA+AGG 0.585722 5:+17420785 MsaT026146.1:CDS
TTGTCAAGTAATGTCAACAA+TGG 0.587069 5:+17419994 MsaT026146.1:CDS
ATATCAGGTCCCATTATACA+TGG 0.589693 5:-17421574 None:intergenic
GGTGGTGCCATTGTTTGGAG+TGG 0.590059 5:-17422546 None:intergenic
TGTCAGCAAAATGCCGAGGA+AGG 0.592338 5:+17423581 MsaT026146.1:CDS
ATTATGTATGCAGGTAACAT+GGG 0.592654 5:+17423809 MsaT026146.1:intron
TGAAAGGGTTGCAAGCCTCA+TGG 0.593187 5:+17423944 MsaT026146.1:CDS
AAATGCCGAGGAAGGAACCT+GGG 0.596467 5:+17423589 MsaT026146.1:CDS
ATCTGTGGCGTTCGTCACGA+AGG 0.596909 5:-17424312 None:intergenic
AGGAAAAGGTTGAAAAGGCA+TGG 0.599322 5:-17420092 None:intergenic
ATGTCTAAGTCCAGTGATGG+TGG 0.600221 5:-17422582 None:intergenic
AAATGCCCGGTTAAAAGAAG+AGG 0.603680 5:+17421627 MsaT026146.1:CDS
ATTGAAGGTGCTCTTTGTGG+CGG 0.608550 5:+17425078 MsaT026146.1:CDS
TTGGCACTAGTAGTAGATGA+TGG 0.610277 5:-17424928 None:intergenic
TTTGATGCAGGGTATTGCAA+AGG 0.610933 5:-17425056 None:intergenic
TGATTGCTGTGACACTTGAA+AGG 0.612049 5:-17425026 None:intergenic
AAGTAATGTCAACAATGGGA+GGG 0.615730 5:+17419999 MsaT026146.1:CDS
CCACCCTCTTGAAAATGTGA+TGG 0.615906 5:-17422681 None:intergenic
TGGCATTTGGTGCACAACGT+TGG 0.617325 5:+17423898 MsaT026146.1:CDS
GGCACTATCATGAACCCTTG+TGG 0.620159 5:-17424853 None:intergenic
TCGTAGCGGAGAGAATGATA+GGG 0.620825 5:+17422806 MsaT026146.1:CDS
AGGAGTGCACACTACTTGGA+GGG 0.620960 5:-17425153 None:intergenic
CATGCCAAATGGCTATTCAA+GGG 0.621111 5:+17423706 MsaT026146.1:CDS
GTGATTCAAGACATGCCAAA+TGG 0.625125 5:+17423695 MsaT026146.1:CDS
AAGAAAATGAGTCTCTCTGG+TGG 0.626409 5:-17423552 None:intergenic
TCTGAAACCAGAACTTAACC+TGG 0.629926 5:-17420764 None:intergenic
TGGTCGTCCACTCCAAACAA+TGG 0.632170 5:+17422539 MsaT026146.1:CDS
GCGGCGAGAATCACTCCACT+GGG 0.633627 5:-17424241 None:intergenic
CTTGTCCCAAATACTGCAGG+AGG 0.633782 5:+17423660 MsaT026146.1:CDS
TGTATAACATCAAGATACAC+TGG 0.638243 5:+17422519 MsaT026146.1:CDS
CTGATATGTTTCTAGCCATG+AGG 0.641439 5:-17423959 None:intergenic
AGAAACATATCAGATCTTGG+AGG 0.641979 5:+17423969 MsaT026146.1:CDS
TCGGTCCAACTAGCTATGAG+TGG 0.646383 5:+17424802 MsaT026146.1:CDS
GGAAGAAGTGCAATGCATGA+AGG 0.647602 5:-17424832 None:intergenic
ACACCGTTAGAATCACAACT+AGG 0.649395 5:+17424197 MsaT026146.1:CDS
TCTCCGCTACGAATCCAAAG+TGG 0.653060 5:-17422795 None:intergenic
AAGCCAAGTGGTTGAAACTG+CGG 0.657898 5:-17424260 None:intergenic
GCATTGCACTTCTTCCACAA+GGG 0.658072 5:+17424839 MsaT026146.1:CDS
ATCAACAGGAAAATCAACAA+TGG 0.660991 5:-17423612 None:intergenic
ACTGAGCCTGGCCAACCCAG+TGG 0.663353 5:+17424226 MsaT026146.1:CDS
TAGACATGAACATTTATCCG+AGG 0.669126 5:+17422598 MsaT026146.1:CDS
GTCTTGAATCACACAGCCAG+AGG 0.672117 5:-17423684 None:intergenic
GCTGATACATCATCTGAACA+AGG 0.673597 5:+17424892 MsaT026146.1:CDS
GAAGAGATCATGGATCAAAG+TGG 0.674817 5:+17420231 MsaT026146.1:CDS
TTCATGTCTAAGTCCAGTGA+TGG 0.677862 5:-17422585 None:intergenic
TGAAAATGTGATGGTTCAGG+TGG 0.678546 5:-17422672 None:intergenic
AGTGATGGTGGCATAAGAGA+TGG 0.687053 5:-17422570 None:intergenic
GGAATCATTTCCGTGCATGG+TGG 0.702254 5:-17422636 None:intergenic
GATGGTGGCATAAGAGATGG+TGG 0.704606 5:-17422567 None:intergenic
TCTGTGGTACCTGAGATCTG+TGG 0.717292 5:-17424327 None:intergenic
GGTGGCATAAGAGATGGTGG+TGG 0.719463 5:-17422564 None:intergenic
GTATTGTCAGCAAAATGCCG+AGG 0.721851 5:+17423577 MsaT026146.1:CDS
GAACCACTTTGGATTCGTAG+CGG 0.724325 5:+17422792 MsaT026146.1:CDS
ATGAGGATAAGGAAGCCAAG+TGG 0.750424 5:-17424272 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 17419983 17425164 17419983 ID=MsaG026146
Chr5 mRNA 17419983 17425164 17419983 ID=MsaT026146.1;Parent=MsaG026146
Chr5 exon 17419983 17420123 17419983 ID=MsaT026146.1.exon1;Parent=MsaT026146.1
Chr5 CDS 17419983 17420123 17419983 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Chr5 exon 17420201 17420376 17420201 ID=MsaT026146.1.exon2;Parent=MsaT026146.1
Chr5 CDS 17420201 17420376 17420201 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Chr5 exon 17420689 17420806 17420689 ID=MsaT026146.1.exon3;Parent=MsaT026146.1
Chr5 CDS 17420689 17420806 17420689 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Chr5 exon 17421457 17421648 17421457 ID=MsaT026146.1.exon4;Parent=MsaT026146.1
Chr5 CDS 17421457 17421648 17421457 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Chr5 exon 17422504 17422974 17422504 ID=MsaT026146.1.exon5;Parent=MsaT026146.1
Chr5 CDS 17422504 17422974 17422504 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Chr5 exon 17423082 17423183 17423082 ID=MsaT026146.1.exon6;Parent=MsaT026146.1
Chr5 CDS 17423082 17423183 17423082 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Chr5 exon 17423515 17423727 17423515 ID=MsaT026146.1.exon7;Parent=MsaT026146.1
Chr5 CDS 17423515 17423727 17423515 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Chr5 exon 17423822 17423990 17423822 ID=MsaT026146.1.exon8;Parent=MsaT026146.1
Chr5 CDS 17423822 17423990 17423822 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Chr5 exon 17424071 17424339 17424071 ID=MsaT026146.1.exon9;Parent=MsaT026146.1
Chr5 CDS 17424071 17424339 17424071 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Chr5 exon 17424517 17424612 17424517 ID=MsaT026146.1.exon10;Parent=MsaT026146.1
Chr5 CDS 17424517 17424612 17424517 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Chr5 exon 17424703 17425164 17424703 ID=MsaT026146.1.exon11;Parent=MsaT026146.1
Chr5 CDS 17424703 17425164 17424703 ID=cds.MsaT026146.1;Parent=MsaT026146.1
Gene Sequence

>MsaT026146.1

ATGTATGGTGATTGTCAAGTAATGTCAACAATGGGAGGGAATGTAGTAGTAAACTCAGAATCTCTCTTCTCTTCTCCGATCCATAACTCTAACTTCAACTTCATGTCAACCATGCCTTTTCAACCTTTTCCTTCTTCCATGAAAGAAGAAGAAGGAATATTGAGAGGTAAAGAAGAGATCATGGATCAAAGTGGTTCAGGAAGTGAACAAGTTGAAGATAAGTCAGGAAATGAACAAGAGATTAATAATGAACAACAGGCTACAAAGAAGAAACGTTATCATAGACACACTGCTCGTCAGATCCAAGAAATGGAAGCTTTGTTCAAGGAATGTCCACACCCAGATGATAAACAAAGACTGAAACTAAGCCATGATTTGGGACTGAAACCACGCCAGGTTAAGTTCTGGTTTCAGAATCGTCGAACTCAGATGAAGGCACAACAAGATCGTTCTGATAACGTGATACTGAGAGCAGAGAATGAGAGTTTGAAGAATGAGAACTATAGGCTACAATCGGCGTTACGTAATATTTTATGTCCTAATTGTGGTGGGCCATGTATAATGGGACCTGATATGGGCTTTGATGATCATCAACTTCGATTTGAAAATGCCCGGTTAAAAGAAGAGCTAGAACGTGTGTGTTGTATAACATCAAGATACACTGGTCGTCCACTCCAAACAATGGCACCACCACCATCTCTTATGCCACCATCACTGGACTTAGACATGAACATTTATCCGAGGCACTTCGATCCAATGCCACCATGCACGGAAATGATTCCTGTCCCTATGTTACCACCTGAACCATCACATTTTCAAGAGGGTGGTCTCTTATTAATGGAAGATGAAAAATCACTTGCAATGGAACTTGCAGCTTCTTCTATGGCTGAGCTTGTTAAGATGTGTCAAATGAACGAACCACTTTGGATTCGTAGCGGAGAGAATGATAGGGAAGTTCTTAATTTTGATGAGCATGCTAGGGTTTTTCAATGGCCTTTGAATCTTAAGCAAAGGAATGAGTTAAGGAATGAAGCTACACGTGATTCTGCTGTTGTTATCATGAATAGTGTCACTCTTGTTGATGCTTTTCTTGATGCTCAAAAATGGATGGAATTGTTTCCAACAATTGTTGCTAGAGCAAGAACTGTTCAAATCATAGCTTCAGGTGCTTCTGGCCATGCAAGTGGGACTCTTCAGCTGATGCACGCAGAATTCCAAGTTCTTTCTCCATTGGTATCCACCAGAGAGACTCATTTTCTTCGGTATTGTCAGCAAAATGCCGAGGAAGGAACCTGGGCCATTGTTGATTTTCCTGTTGATAGCTTCCACCAAAACTTTCAAAATTCTTGTCCCAAATACTGCAGGAGGTCCTCTGGCTGTGTGATTCAAGACATGCCAAATGGCTATTCAAGGGTAACATGGGTTGAGCATGCAAAAGTTGTAGAGAAACCAGTCCATCAAATATTTAGTAACTATGTGTATAGTGGAATGGCATTTGGTGCACAACGTTGGTTAGGAGTTTTGCAGAGGCAATGTGAAAGGGTTGCAAGCCTCATGGCTAGAAACATATCAGATCTTGGAGTGATACCTTCTCCGGAAGCGCGAAAGAGCTTGATGAAATTAGCAAATAGGATGATAAAAACTTTTAGCCTTAATATGACCACTTGTGGTGAACAATCATGGACTGCTATATCGGATTCACCAGAAGACACCGTTAGAATCACAACTAGGAAAATCACTGAGCCTGGCCAACCCAGTGGAGTGATTCTCGCCGCAGTTTCAACCACTTGGCTTCCTTATCCTCATACCAAAGTCTTTGATCTCCTTCGTGACGAACGCCACAGATCTCAGATGGATGCACTTTCTAATGGGAACTCATTAAATGAAGTGGCTCATATTGCTAACGGTTCACATCCGGGAAACTGCATTTCCCTTCTTCGCATAAATGTAGCAAGCAACTCATCTCAAAATGTAGAGCTAATGCTGCAAGAGAGTTGCACAGATCAATCTGGAAGCTTAGTAGTCTACACGACAGTCGATGTTGATTCGGTCCAACTAGCTATGAGTGGACAAGATCCTTCATGCATTGCACTTCTTCCACAAGGGTTCATGATAGTGCCAATGGTTTCATCAAATGCTGATACATCATCTGAACAAGGTGTCACAGGAACACCATCATCTACTACTAGTGCCAATGCTGCTAACTCAGGTTGTCTTCTTATCATGGGAATGCAAGTTTTAGCAAGCACAATTCCTTCTGCAAAGCTTAACCTTTCAAGTGTCACAGCAATCAATAATCACCTTTGCAATACCCTGCATCAAATTGAAGGTGCTCTTTGTGGCGGCACTAACAGCAATTGCTTTGTTGGGTCTTGCAATGAACCAACCACTAGTGCACCCTCCAAGTAG
Protein sequence

>MsaT026146.1

MYGDCQVMSTMGGNVVVNSESLFSSPIHNSNFNFMSTMPFQPFPSSMKEEEGILRGKEEIMDQSGSGSEQVEDKSGNEQEINNEQQATKKKRYHRHTARQIQEMEALFKECPHPDDKQRLKLSHDLGLKPRQVKFWFQNRRTQMKAQQDRSDNVILRAENESLKNENYRLQSALRNILCPNCGGPCIMGPDMGFDDHQLRFENARLKEELERVCCITSRYTGRPLQTMAPPPSLMPPSLDLDMNIYPRHFDPMPPCTEMIPVPMLPPEPSHFQEGGLLLMEDEKSLAMELAASSMAELVKMCQMNEPLWIRSGENDREVLNFDEHARVFQWPLNLKQRNELRNEATRDSAVVIMNSVTLVDAFLDAQKWMELFPTIVARARTVQIIASGASGHASGTLQLMHAEFQVLSPLVSTRETHFLRYCQQNAEEGTWAIVDFPVDSFHQNFQNSCPKYCRRSSGCVIQDMPNGYSRVTWVEHAKVVEKPVHQIFSNYVYSGMAFGAQRWLGVLQRQCERVASLMARNISDLGVIPSPEARKSLMKLANRMIKTFSLNMTTCGEQSWTAISDSPEDTVRITTRKITEPGQPSGVILAAVSTTWLPYPHTKVFDLLRDERHRSQMDALSNGNSLNEVAHIANGSHPGNCISLLRINVASNSSQNVELMLQESCTDQSGSLVVYTTVDVDSVQLAMSGQDPSCIALLPQGFMIVPMVSSNADTSSEQGVTGTPSSTTSANAANSGCLLIMGMQVLASTIPSAKLNLSSVTAINNHLCNTLHQIEGALCGGTNSNCFVGSCNEPTTSAPSK*