AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG026195

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026195 MtrunA17_Chr5g0411341 95.168 683 21 3 1 677 1 677 0.0 1330
MsaG026195 MtrunA17_Chr8g0366751 60.838 692 207 17 2 677 3 646 0.0 751
MsaG026195 MtrunA17_Chr5g0411331 81.277 235 43 1 21 254 4 238 1.12e-137 404
MsaG026195 MtrunA17_Chr8g0346161 50.000 202 90 4 474 674 272 463 8.84e-54 191
MsaG026195 MtrunA17_Chr8g0346161 32.796 186 92 2 44 225 25 181 3.53e-30 124
MsaG026195 MtrunA17_Chr8g0346151 51.980 202 88 4 474 673 260 454 2.09e-53 190
MsaG026195 MtrunA17_Chr8g0346151 30.319 188 83 3 58 236 36 184 4.49e-25 108
MsaG026195 MtrunA17_Chr7g0260841 43.541 209 79 3 466 670 328 501 1.44e-41 158
MsaG026195 MtrunA17_Chr7g0260841 28.723 188 118 5 44 225 49 226 2.99e-14 75.9
MsaG026195 MtrunA17_Chr7g0275821 39.834 241 119 6 441 677 200 418 1.42e-35 139
MsaG026195 MtrunA17_Chr1g0185621 35.789 190 106 6 45 225 11 193 1.97e-27 118
MsaG026195 MtrunA17_Chr1g0185621 31.217 189 116 4 471 655 456 634 1.34e-18 90.1
MsaG026195 MtrunA17_Chr8g0386921 35.260 173 97 5 60 222 19 186 5.64e-25 110
MsaG026195 MtrunA17_Chr8g0386921 46.377 69 35 1 489 555 402 470 1.02e-11 68.2
MsaG026195 MtrunA17_Chr5g0400671 34.810 158 85 3 482 639 147 286 3.37e-18 86.3
MsaG026195 MtrunA17_Chr2g0333641 25.911 247 156 5 395 640 51 271 7.02e-17 82.0
MsaG026195 MtrunA17_Chr4g0033391 31.707 164 89 5 480 640 137 280 1.15e-16 81.6
MsaG026195 MtrunA17_Chr2g0280161 27.313 227 128 7 421 639 116 313 1.42e-16 82.0
MsaG026195 MtrunA17_Chr3g0131691 31.000 200 106 6 491 674 296 479 3.77e-16 81.6
MsaG026195 MtrunA17_Chr4g0028071 30.488 164 100 4 483 645 142 292 3.97e-16 80.1
MsaG026195 MtrunA17_Chr7g0225901 31.056 161 100 2 493 653 88 237 5.23e-16 78.6
MsaG026195 MtrunA17_Chr4g0054581 30.481 187 106 4 479 659 190 358 1.42e-15 79.3
MsaG026195 MtrunA17_Chr4g0033291 27.753 227 127 5 414 640 99 288 2.15e-15 78.2
MsaG026195 MtrunA17_Chr4g0049331 31.579 152 87 2 491 639 133 270 2.29e-15 77.8
MsaG026195 MtrunA17_Chr4g0033281 28.155 206 113 4 435 640 115 285 3.11e-15 77.4
MsaG026195 MtrunA17_Chr0c01g0489461 26.906 223 122 8 470 663 170 380 3.79e-15 78.2
MsaG026195 MtrunA17_Chr2g0307131 30.539 167 98 3 476 640 165 315 5.13e-15 77.0
MsaG026195 MtrunA17_Chr2g0307141 29.333 150 91 2 493 640 183 319 1.77e-14 75.5
MsaG026195 MtrunA17_Chr4g0054571 30.000 170 92 3 469 616 105 269 2.13e-14 75.1
MsaG026195 MtrunA17_Chr5g0400621 30.723 166 100 4 476 640 142 293 3.07e-14 74.7
MsaG026195 MtrunA17_Chr2g0320291 30.435 161 95 5 497 655 137 282 4.09e-14 73.9
MsaG026195 MtrunA17_Chr4g0002781 29.878 164 99 3 481 640 162 313 4.42e-14 74.3
MsaG026195 MtrunA17_Chr2g0333621 27.684 177 108 3 469 640 114 275 7.45e-14 73.2
MsaG026195 MtrunA17_Chr1g0211081 27.650 217 132 6 472 669 301 511 1.14e-13 73.9
MsaG026195 MtrunA17_Chr4g0002801 30.286 175 87 5 479 640 142 294 1.19e-13 72.8
MsaG026195 MtrunA17_Chr5g0400641 30.872 149 92 2 497 645 2 139 1.29e-13 69.3
MsaG026195 MtrunA17_Chr2g0298751 31.373 153 97 3 494 641 53 202 1.54e-13 70.9
MsaG026195 MtrunA17_Chr6g0468491 32.903 155 79 4 493 640 137 273 1.76e-13 72.0
MsaG026195 MtrunA17_Chr4g0054561 31.765 170 88 7 474 640 140 284 2.56e-13 71.6
MsaG026195 MtrunA17_Chr5g0400661 26.009 223 134 6 463 670 115 321 3.79e-13 71.2
MsaG026195 MtrunA17_Chr8g0366551 28.409 176 113 3 492 659 124 294 7.31e-13 70.1
MsaG026195 MtrunA17_Chr5g0426851 27.273 209 135 7 477 669 78 285 8.92e-13 69.7
MsaG026195 MtrunA17_Chr8g0365711 29.282 181 107 6 475 649 234 399 9.61e-13 70.9
MsaG026195 MtrunA17_Chr7g0270871 29.016 193 133 2 352 543 73 262 1.27e-12 70.1
MsaG026195 MtrunA17_Chr7g0252041 55.769 52 23 0 491 542 284 335 2.85e-12 69.7
MsaG026195 MtrunA17_Chr8g0336681 26.816 179 111 3 470 640 133 299 4.41e-12 68.2
MsaG026195 MtrunA17_Chr6g0468461 55.556 54 24 0 493 546 173 226 8.88e-12 67.4
MsaG026195 MtrunA17_Chr4g0052821 26.263 198 126 4 487 676 284 469 9.83e-12 67.8
MsaG026195 MtrunA17_Chr4g0054551 31.788 151 79 6 493 640 155 284 1.45e-11 66.2
MsaG026195 MtrunA17_Chr5g0411541 28.814 177 99 4 481 652 141 295 4.27e-11 64.7
MsaG026195 MtrunA17_Chr2g0333611 29.605 152 84 4 494 640 46 179 4.51e-11 63.2
MsaG026195 MtrunA17_Chr4g0054621 28.859 149 76 4 493 635 172 296 4.87e-11 64.7
MsaG026195 MtrunA17_Chr4g0052841 28.796 191 116 5 494 676 293 471 6.09e-11 65.5
MsaG026195 MtrunA17_Chr1g0158941 26.289 194 108 5 492 656 87 274 7.08e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026195 AT1G32640.1 52.192 707 212 21 1 674 10 623 0.0 617
MsaG026195 AT5G46760.1 50.511 685 222 18 3 675 11 590 0.0 538
MsaG026195 AT4G17880.1 64.552 268 64 6 410 676 351 588 4.96e-105 332
MsaG026195 AT4G17880.1 58.303 271 99 5 2 263 22 287 1.29e-93 301
MsaG026195 AT5G46830.1 54.974 191 71 3 484 673 334 510 1.36e-54 195
MsaG026195 AT5G46830.1 46.218 238 107 6 5 231 4 231 1.78e-54 195
MsaG026195 AT1G01260.1 40.741 243 99 5 433 670 373 575 1.00e-41 160
MsaG026195 AT1G01260.1 31.169 231 150 3 3 225 7 236 1.77e-29 124
MsaG026195 AT1G01260.2 40.741 243 99 5 433 670 373 575 1.00e-41 160
MsaG026195 AT1G01260.2 31.169 231 150 3 3 225 7 236 1.77e-29 124
MsaG026195 AT1G01260.3 40.741 243 99 5 433 670 373 575 1.00e-41 160
MsaG026195 AT1G01260.3 31.169 231 150 3 3 225 7 236 1.77e-29 124
MsaG026195 AT2G46510.1 42.534 221 89 4 459 677 368 552 3.02e-41 159
MsaG026195 AT2G46510.1 33.476 233 143 4 4 225 8 239 2.85e-27 117
MsaG026195 AT4G16430.1 41.799 189 76 2 466 654 293 447 1.00e-37 147
MsaG026195 AT4G16430.1 29.348 184 120 4 45 225 50 226 7.66e-14 74.7
MsaG026195 AT4G00870.1 44.828 203 92 5 478 669 224 417 1.10e-35 140
MsaG026195 AT4G00870.1 31.148 183 98 4 44 225 34 189 3.90e-20 94.0
MsaG026195 AT4G09820.1 33.333 204 115 6 33 225 8 201 2.44e-26 114
MsaG026195 AT4G09820.1 31.373 153 74 3 490 642 360 481 2.13e-15 80.1
MsaG026195 AT1G63650.3 33.831 201 106 6 43 225 11 202 4.05e-23 104
MsaG026195 AT1G63650.3 26.455 189 131 3 489 669 401 589 1.44e-14 77.8
MsaG026195 AT1G63650.1 33.831 201 106 6 43 225 11 202 4.05e-23 104
MsaG026195 AT1G63650.1 26.455 189 131 3 489 669 401 589 1.44e-14 77.8
MsaG026195 AT1G63650.2 33.831 201 106 6 43 225 11 202 4.05e-23 104
MsaG026195 AT1G63650.2 26.455 189 131 3 489 669 401 589 1.44e-14 77.8
MsaG026195 AT5G41315.1 30.097 206 124 6 38 225 3 206 2.22e-20 96.3
MsaG026195 AT5G41315.1 25.758 198 130 4 487 669 435 630 1.07e-12 71.6
MsaG026195 AT5G41315.2 30.097 206 124 6 38 225 11 214 2.32e-20 96.3
MsaG026195 AT5G41315.2 25.758 198 130 4 487 669 443 638 9.79e-13 72.0
MsaG026195 AT5G41315.3 30.097 206 124 6 38 225 8 211 2.68e-20 95.9
MsaG026195 AT5G41315.3 25.758 198 130 4 487 669 440 635 1.16e-12 71.6
MsaG026195 AT4G00480.3 32.240 183 100 4 60 222 36 214 1.90e-19 92.8
MsaG026195 AT4G00480.1 32.240 183 100 4 60 222 36 214 2.22e-19 92.4
MsaG026195 AT4G00480.2 32.240 183 100 4 60 222 36 214 3.09e-19 92.4
MsaG026195 AT4G37850.2 32.973 185 92 6 471 640 123 290 1.21e-17 85.1
MsaG026195 AT4G37850.1 32.973 185 92 6 471 640 123 290 1.21e-17 85.1
MsaG026195 AT5G57150.1 30.526 190 110 6 494 674 55 231 2.20e-16 79.7
MsaG026195 AT2G28160.1 32.877 146 88 4 497 641 135 271 4.97e-16 80.1
MsaG026195 AT2G28160.2 32.877 146 88 4 497 641 135 271 6.60e-16 79.7
MsaG026195 AT5G57150.3 29.651 172 101 5 494 656 56 216 9.74e-16 77.4
MsaG026195 AT5G57150.2 29.651 172 101 5 494 656 56 216 1.37e-15 77.0
MsaG026195 AT5G57150.5 29.825 171 100 5 494 655 55 214 1.88e-15 76.6
MsaG026195 AT5G57150.4 29.545 176 104 5 494 660 56 220 2.01e-15 77.4
MsaG026195 AT2G22750.4 31.288 163 104 3 479 640 140 295 3.31e-14 74.7
MsaG026195 AT3G24140.1 25.592 211 132 6 475 663 177 384 3.62e-14 75.5
MsaG026195 AT2G22750.3 31.288 163 104 3 479 640 140 295 3.62e-14 74.7
MsaG026195 AT2G22750.5 30.488 164 104 4 479 640 136 291 3.68e-14 74.7
MsaG026195 AT2G22750.1 31.707 164 102 4 479 640 112 267 3.76e-14 74.3
MsaG026195 AT2G22750.2 31.707 164 102 4 479 640 112 267 4.21e-14 74.3
MsaG026195 AT3G24140.2 25.592 211 132 6 475 663 177 384 5.67e-14 74.7
MsaG026195 AT4G29930.6 32.867 143 78 3 494 635 55 180 6.58e-14 72.0
MsaG026195 AT3G26744.6 30.899 178 108 6 491 662 305 473 9.03e-14 74.7
MsaG026195 AT4G29930.5 32.168 143 76 3 494 635 55 177 2.12e-13 70.9
MsaG026195 AT4G29930.3 28.662 157 92 3 494 635 55 206 2.33e-13 71.2
MsaG026195 AT4G29930.7 28.662 157 92 3 494 635 55 206 2.33e-13 71.2
MsaG026195 AT3G26744.1 31.707 164 99 5 491 648 305 461 2.34e-13 73.6
MsaG026195 AT3G26744.4 31.707 164 99 5 491 648 305 461 2.34e-13 73.6
MsaG026195 AT3G26744.2 31.707 164 99 5 491 648 305 461 2.34e-13 73.6
MsaG026195 AT1G12860.2 30.769 169 90 4 491 648 265 417 2.69e-13 72.8
MsaG026195 AT1G12860.1 29.442 197 110 5 491 674 265 445 3.24e-13 72.8
MsaG026195 AT4G29930.2 32.168 143 76 3 494 635 55 177 3.26e-13 70.1
MsaG026195 AT4G29930.1 28.662 157 92 3 494 635 55 206 4.02e-13 70.5
MsaG026195 AT4G29930.4 44.000 75 42 0 495 569 56 130 8.60e-13 67.8
MsaG026195 AT2G16910.1 33.136 169 76 6 497 641 318 473 1.03e-12 71.6
MsaG026195 AT2G22770.1 26.486 185 120 4 487 659 123 303 5.25e-12 68.2
MsaG026195 AT2G22760.2 26.638 229 121 8 420 640 69 258 8.89e-12 67.0
MsaG026195 AT2G22760.1 28.780 205 99 8 420 616 69 234 9.47e-12 67.0
MsaG026195 AT5G65640.1 30.000 200 116 7 479 674 166 345 2.68e-11 66.2

Find 164 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATCGATGTTAAGATTAT+AGG 0.170893 5:+18374138 MsaT026195.1:CDS
CATTTCATTTCATGAATCTT+TGG 0.223803 5:+18372336 None:intergenic
ACAACAAGCAACCGTGAAAA+TGG 0.224565 5:+18374290 MsaT026195.1:CDS
TGATTCGAAGAGGGTTTATT+TGG 0.231042 5:-18374099 None:intergenic
AGTTCTTATGTTGCTAATAA+TGG 0.260796 5:+18373463 MsaT026195.1:CDS
CAACCTTCTTACGACTATTC+AGG 0.266356 5:+18372545 MsaT026195.1:CDS
GAAATGAATCTTTCTGATTA+TGG 0.280620 5:+18373367 MsaT026195.1:CDS
GATGGATAAAGCTTCACTTT+TGG 0.289543 5:+18373909 MsaT026195.1:CDS
CACAAAATCTCACCGGATTC+CGG 0.303867 5:-18373421 None:intergenic
AGGTTCTTCGTTACTTGGTT+GGG 0.304524 5:+18372565 MsaT026195.1:CDS
TATTCAGGTTCTTCGTTACT+TGG 0.315314 5:+18372560 MsaT026195.1:CDS
ACCATCGGCTGACGGTGTTT+TGG 0.319919 5:+18372934 MsaT026195.1:CDS
TGTTCTGTTGTTGGTGCTGC+TGG 0.321937 5:-18373207 None:intergenic
TTGAACATCCCCGACTTTAG+AGG 0.323506 5:-18374355 None:intergenic
AGAATGATCCTTCTTCGATT+TGG 0.325148 5:+18373086 MsaT026195.1:CDS
CCACCCGGCAGCAAGGTTAA+TGG 0.328981 5:+18374200 MsaT026195.1:CDS
TTCTTCTGTAACCGGATTAC+CGG 0.334316 5:-18372705 None:intergenic
TTTGGATCTTCTTGGCAGTT+AGG 0.342187 5:+18373043 MsaT026195.1:CDS
CACTGGCATGGTGCACTTCT+AGG 0.343448 5:-18374242 None:intergenic
TATTGTTATTGTTCTGTTGT+TGG 0.349299 5:-18373216 None:intergenic
CCTCTGATTTATCAACCTTA+TGG 0.351613 5:+18372399 MsaT026195.1:CDS
GGTTCCTCTCTTCCGTTTGC+CGG 0.358134 5:-18373796 None:intergenic
CTCCGGTGTTCTTGTTCAGC+AGG 0.359735 5:-18372650 None:intergenic
ACAGAACAATAACAATAATC+AGG 0.363674 5:+18373222 MsaT026195.1:CDS
TTATCCATCTTTGAAACATT+AGG 0.366548 5:-18373895 None:intergenic
TGGGGTGATGGTTACTATAA+AGG 0.374935 5:+18372584 MsaT026195.1:CDS
AAGGTTAATGGCAGCGTTAA+TGG 0.379124 5:+18374212 MsaT026195.1:CDS
TCGTTACTTGGTTGGGGTGA+TGG 0.379647 5:+18372572 MsaT026195.1:CDS
CAGGTTCTTCGTTACTTGGT+TGG 0.382764 5:+18372564 MsaT026195.1:CDS
TTGAAATTCTCTGGTGGTAC+TGG 0.385403 5:+18373667 MsaT026195.1:CDS
TGTTGGTGCTGCTGGTGACT+TGG 0.388845 5:-18373199 None:intergenic
TTCATTAATCTCCGGTAATC+CGG 0.396662 5:+18372694 MsaT026195.1:CDS
TCAGCTCCGGTGTGGTTAAC+CGG 0.398598 5:+18372833 MsaT026195.1:CDS
TCGAATTCGAATTTCTTCTC+TGG 0.399599 5:+18373490 MsaT026195.1:CDS
TTATTGCTTCCCCTCGAATT+CGG 0.403187 5:-18373586 None:intergenic
ACAGGAGATTCTTCTGTAAC+CGG 0.415426 5:-18372713 None:intergenic
TTAAAGCTTGAAGACGTTGT+TGG 0.415771 5:-18372481 None:intergenic
TGTTCCTAATGTTTCAAAGA+TGG 0.422462 5:+18373891 MsaT026195.1:CDS
CAACAAGCAACCGTGAAAAT+GGG 0.427408 5:+18374291 MsaT026195.1:CDS
GCGTCGAATTTGAAATTCTC+TGG 0.430033 5:+18373658 MsaT026195.1:CDS
TCAACAGAATCTCTAGCTTC+AGG 0.431024 5:-18373118 None:intergenic
GTTGTGGTGGCTGATGAGAC+TGG 0.431357 5:-18374053 None:intergenic
GTGGTTTGTGGTGGTTGTGA+CGG 0.435088 5:-18372434 None:intergenic
GGTGGTTGTGACGGTGGTGC+TGG 0.437264 5:-18372425 None:intergenic
TTGAATCCGGTGGTGGTTTG+TGG 0.438183 5:-18372446 None:intergenic
GAAGAGGGTTTATTTGGTAC+TGG 0.440122 5:-18374093 None:intergenic
TCAGTAACTTCTTCATCAAC+AGG 0.444377 5:-18372731 None:intergenic
TTCTCTGGTGGTACTGGTGG+TGG 0.450615 5:+18373673 MsaT026195.1:CDS
GGCTTCCTCCCTCTCTTCCT+CGG 0.451066 5:-18373775 None:intergenic
GAAAAGAAGCCGAGGAAGAG+AGG 0.453055 5:+18373766 MsaT026195.1:CDS
GAGGAAGAGAGGGAGGAAGC+CGG 0.453227 5:+18373777 MsaT026195.1:CDS
GTCAACGGAACTGGGCTGCC+TGG 0.453582 5:+18372794 MsaT026195.1:CDS
TGGTACTGGTGGTGGTGATT+CGG 0.453669 5:+18373681 MsaT026195.1:CDS
GAGAGTGTCTTGGTTGAATC+CGG 0.454531 5:-18372459 None:intergenic
TCCTGGTTCGAGTACTTTAA+CGG 0.455187 5:+18373285 MsaT026195.1:CDS
CGGTGGTGCTGGTGGCCATA+AGG 0.456488 5:-18372414 None:intergenic
TCGAAGGCGCAAAAGAAGTC+TGG 0.457045 5:+18372504 MsaT026195.1:CDS
ACAACAATGGGAACGGGAAT+GGG 0.462203 5:+18373545 MsaT026195.1:CDS
CGTTTGTTGAAGCCGGAATC+CGG 0.465479 5:+18373409 MsaT026195.1:CDS
TGGTGGTGATTCGGACCATT+CGG 0.468581 5:+18373690 MsaT026195.1:CDS
TGTTGTTGTTGCTGCTGTTG+TGG 0.469442 5:-18374069 None:intergenic
TGATTCGGACCATTCGGATC+TGG 0.469684 5:+18373696 MsaT026195.1:CDS
CGGCATTGTCCTCTAAAGTC+GGG 0.471070 5:+18374346 MsaT026195.1:CDS
AACAACAATGGGAACGGGAA+TGG 0.482475 5:+18373544 MsaT026195.1:CDS
CCATTCGGATCTGGAGGCTT+CGG 0.482750 5:+18373705 MsaT026195.1:CDS
TAGGCTGGGATGCAATGATA+AGG 0.484555 5:+18374157 MsaT026195.1:CDS
CGCCCTCTCACAAGCTGAGA+GGG 0.484643 5:-18372870 None:intergenic
GGTTCTTCGTTACTTGGTTG+GGG 0.488569 5:+18372566 MsaT026195.1:CDS
AAAACTGAATCCAATAAAGA+TGG 0.488902 5:+18373976 MsaT026195.1:CDS
TCTGGAGGCTTCGGTGGTGA+AGG 0.499262 5:+18373714 MsaT026195.1:CDS
AATGGGAACGGGAATGGGAA+TGG 0.501146 5:+18373550 MsaT026195.1:CDS
AATTTCAAATTCGACGCCCC+TGG 0.511045 5:-18373652 None:intergenic
CGATGTTAAGATTATAGGCT+GGG 0.511425 5:+18374143 MsaT026195.1:CDS
GGCTTCGGTGGTGAAGGAGG+TGG 0.511799 5:+18373720 MsaT026195.1:CDS
AAAAGAAGCCGAGGAAGAGA+GGG 0.514449 5:+18373767 MsaT026195.1:CDS
AGGGGAAGCAATAATGATGA+TGG 0.515247 5:+18373595 MsaT026195.1:CDS
CGCCCTCTCAGCTTGTGAGA+GGG 0.515362 5:+18372868 MsaT026195.1:CDS
CTGCCATTAACCTTGCTGCC+GGG 0.517345 5:-18374203 None:intergenic
AGCTTATTATAATTCAGCTC+CGG 0.519595 5:+18372820 MsaT026195.1:CDS
AGAGGAGATCACCGAATTCG+AGG 0.519707 5:+18373575 MsaT026195.1:CDS
AGAGGGAGGAAGCCGGCAAA+CGG 0.519792 5:+18373784 MsaT026195.1:CDS
CTTGAAACATGAACAACACT+AGG 0.520830 5:-18373313 None:intergenic
GAACTTAATTCATTAATCTC+CGG 0.523787 5:+18372686 MsaT026195.1:CDS
ACGGGAATGGGAATGGAAAG+AGG 0.524874 5:+18373557 MsaT026195.1:CDS
AGGCTGGGATGCAATGATAA+GGG 0.527237 5:+18374158 MsaT026195.1:CDS
AGGAGAACAACAATGGGAAC+GGG 0.528523 5:+18373539 MsaT026195.1:CDS
GTCTTGGTTGAATCCGGTGG+TGG 0.529478 5:-18372453 None:intergenic
GCTGCCATTAACCTTGCTGC+CGG 0.530316 5:-18374204 None:intergenic
AGGATCATTCTCACCATGAT+GGG 0.531136 5:-18373074 None:intergenic
TCTGGTGTAATTGTTCCACC+AGG 0.531186 5:+18373634 MsaT026195.1:CDS
TCAGCAACGTTTGTTGAAGC+CGG 0.534264 5:+18373402 MsaT026195.1:CDS
GAGGAGATCACCGAATTCGA+GGG 0.535922 5:+18373576 MsaT026195.1:CDS
TGTTGTTGCTGCTGTTGTGG+TGG 0.537319 5:-18374066 None:intergenic
AAGGATCATTCTCACCATGA+TGG 0.538289 5:-18373075 None:intergenic
CGACGATAACTCTTCAGTAA+TGG 0.538731 5:+18372361 MsaT026195.1:CDS
TTGCTGAGGAGAACAACAAT+GGG 0.545102 5:+18373533 MsaT026195.1:CDS
GACGTTGTTGGAGAGTGTCT+TGG 0.546035 5:-18372469 None:intergenic
TGGTTCGAGTACTTTAACGG+AGG 0.547059 5:+18373288 MsaT026195.1:CDS
AATCTTTCTGATTATGGTGG+TGG 0.549706 5:+18373373 MsaT026195.1:CDS
TCGTTAATCCAAATCGAAGA+AGG 0.550466 5:-18373094 None:intergenic
ATCATTTACCACAGACACAC+TGG 0.550984 5:-18374259 None:intergenic
TTCAAATTCGACGCCCCTGG+TGG 0.552724 5:-18373649 None:intergenic
TCGCCCTCTCAGCTTGTGAG+AGG 0.553021 5:+18372867 MsaT026195.1:CDS
AAATTCTCTGGTGGTACTGG+TGG 0.553450 5:+18373670 MsaT026195.1:CDS
GAACCTGAATAGTCGTAAGA+AGG 0.553990 5:-18372548 None:intergenic
TCGATGTTAAGATTATAGGC+TGG 0.555177 5:+18374142 MsaT026195.1:CDS
GTTTGTGGTGGTTGTGACGG+TGG 0.556522 5:-18372431 None:intergenic
GGAGGCTTCGGTGGTGAAGG+AGG 0.561351 5:+18373717 MsaT026195.1:CDS
ACGATGCAATTTGAAACTCC+TGG 0.564466 5:+18373268 MsaT026195.1:CDS
GAATTATAATAAGCTTGTCC+AGG 0.566444 5:-18372812 None:intergenic
GGTTGTGACGGTGGTGCTGG+TGG 0.566453 5:-18372422 None:intergenic
TTCGGATCTGGAGGCTTCGG+TGG 0.573741 5:+18373708 MsaT026195.1:CDS
ATTAGAGCTTGATTCGAAGA+GGG 0.573791 5:-18374108 None:intergenic
GAAGCCGGCAAACGGAAGAG+AGG 0.578591 5:+18373792 MsaT026195.1:CDS
CAACCACCACAAACCACCAC+CGG 0.580431 5:+18372440 MsaT026195.1:CDS
TGATGAAGAAGTTACTGATA+CGG 0.580523 5:+18372736 MsaT026195.1:CDS
GCGGCATTGTCCTCTAAAGT+CGG 0.582204 5:+18374345 MsaT026195.1:CDS
AGAAGCCGAGGAAGAGAGGG+AGG 0.582977 5:+18373770 MsaT026195.1:CDS
ACAGTTAGTTTCCGTTGCTG+AGG 0.584195 5:+18373519 MsaT026195.1:CDS
TCGCCCTCTCACAAGCTGAG+AGG 0.584834 5:-18372871 None:intergenic
CGTCTTCAAGCTTTAATCGA+AGG 0.585450 5:+18372488 MsaT026195.1:CDS
CACTCAGGAGCAGCTTCGAG+CGG 0.590406 5:+18374326 MsaT026195.1:CDS
TCAGCTTCAACATGATTCAA+CGG 0.591097 5:-18373817 None:intergenic
CAGAACAATAACAATAATCA+GGG 0.591184 5:+18373223 MsaT026195.1:CDS
AATCCGGTGGTGGTTTGTGG+TGG 0.592017 5:-18372443 None:intergenic
GTTGCTGAGGAGAACAACAA+TGG 0.596228 5:+18373532 MsaT026195.1:CDS
CCATTAACCTTGCTGCCGGG+TGG 0.597585 5:-18374200 None:intergenic
TGGTGTAATTGTTCCACCAG+GGG 0.598619 5:+18373636 MsaT026195.1:CDS
CTGGTGTAATTGTTCCACCA+GGG 0.599733 5:+18373635 MsaT026195.1:CDS
ACCAAAACACAAAATCTCAC+CGG 0.600817 5:-18373428 None:intergenic
CGATAACTCTTCAGTAATGG+AGG 0.601389 5:+18372364 MsaT026195.1:CDS
TCCGTTAAAGTACTCGAACC+AGG 0.602105 5:-18373286 None:intergenic
ATTGTTGTTCTCCTCAGCAA+CGG 0.603161 5:-18373530 None:intergenic
TTCGGACCATTCGGATCTGG+AGG 0.603525 5:+18373699 MsaT026195.1:CDS
GGTGGATAGTAGTCGTGTGG+TGG 0.606483 5:+18373738 MsaT026195.1:CDS
GAGGAGAACAACAATGGGAA+CGG 0.611347 5:+18373538 MsaT026195.1:CDS
TTAGGTAATTCTGCCCATCA+TGG 0.611875 5:+18373061 MsaT026195.1:CDS
TCCAAAACACCGTCAGCCGA+TGG 0.615737 5:-18372935 None:intergenic
GATTAGAGCTTGATTCGAAG+AGG 0.620386 5:-18374109 None:intergenic
TCAGCTCCGGTTAACCACAC+CGG 0.622205 5:-18372839 None:intergenic
GCGAGACAAGGTCAAGAACA+TGG 0.625791 5:+18372890 MsaT026195.1:CDS
AACAAGCAACCGTGAAAATG+GGG 0.625969 5:+18374292 MsaT026195.1:CDS
GCTTGTGAGAGGGCGAGACA+AGG 0.630681 5:+18372878 MsaT026195.1:CDS
TCGAATTTGAAATTCTCTGG+TGG 0.634544 5:+18373661 MsaT026195.1:CDS
ATTATAATTCAGCTCCGGTG+TGG 0.637766 5:+18372825 MsaT026195.1:CDS
CACCTGCTGAACAAGAACAC+CGG 0.639706 5:+18372648 MsaT026195.1:CDS
GGCATTGTCCTCTAAAGTCG+GGG 0.642951 5:+18374347 MsaT026195.1:CDS
GCGTGTATACCATCGGCTGA+CGG 0.644460 5:+18372926 MsaT026195.1:CDS
GACGCTGGCGTGTATACCAT+CGG 0.650932 5:+18372919 MsaT026195.1:CDS
TGAATCATGTTGAAGCTGAG+AGG 0.655301 5:+18373821 MsaT026195.1:CDS
AGTGTCTTGGTTGAATCCGG+TGG 0.657627 5:-18372456 None:intergenic
TTACCACAGACACACTGGCA+TGG 0.658103 5:-18374254 None:intergenic
ATGAATCTTTCTGATTATGG+TGG 0.662194 5:+18373370 MsaT026195.1:CDS
GCACCATGCCAGTGTGTCTG+TGG 0.666701 5:+18374251 MsaT026195.1:CDS
AGGTTGCCAGAAAATAGCGT+AGG 0.667613 5:-18372528 None:intergenic
AGGAGATCACCGAATTCGAG+GGG 0.671847 5:+18373577 MsaT026195.1:CDS
TAAGGTTGATAAATCAGAGG+AGG 0.672550 5:-18372396 None:intergenic
CCATAAGGTTGATAAATCAG+AGG 0.672761 5:-18372399 None:intergenic
AGAACATGGTATACAGACGC+TGG 0.675420 5:+18372904 MsaT026195.1:CDS
GGAGGTGGATAGTAGTCGTG+TGG 0.676666 5:+18373735 MsaT026195.1:CDS
CCGAAGCCTCCAGATCCGAA+TGG 0.681912 5:-18373705 None:intergenic
TCTTGTTACAACAACAACAA+CGG 0.685083 5:+18373150 MsaT026195.1:CDS
TGGAGCCCGAAAAGAAGCCG+AGG 0.695389 5:+18373758 MsaT026195.1:CDS
AAGAAGTTACTGATACGGAG+TGG 0.724771 5:+18372741 MsaT026195.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 18372347 18374380 18372347 ID=MsaG026195
Chr5 mRNA 18372347 18374380 18372347 ID=MsaT026195.1;Parent=MsaG026195
Chr5 exon 18372347 18374380 18372347 ID=MsaT026195.1.exon1;Parent=MsaT026195.1
Chr5 CDS 18372347 18374380 18372347 ID=cds.MsaT026195.1;Parent=MsaT026195.1
Gene Sequence

>MsaT026195.1

ATGAATCTTTGGAGCGACGATAACTCTTCAGTAATGGAGGCTTTCATGACCTCCTCTGATTTATCAACCTTATGGCCACCAGCACCACCGTCACAACCACCACAAACCACCACCGGATTCAACCAAGACACTCTCCAACAACGTCTTCAAGCTTTAATCGAAGGCGCAAAAGAAGTCTGGACCTACGCTATTTTCTGGCAACCTTCTTACGACTATTCAGGTTCTTCGTTACTTGGTTGGGGTGATGGTTACTATAAAGGCGAAGAAGACAAAACAAAAGCGAAGAAATCAAAAGTTACTTCACCTGCTGAACAAGAACACCGGAGAAAAGTTCTTAGAGAACTTAATTCATTAATCTCCGGTAATCCGGTTACAGAAGAATCTCCTGTTGATGAAGAAGTTACTGATACGGAGTGGTTCTTTTTAGTTTCTATGACGCAGTCTTTTGTCAACGGAACTGGGCTGCCTGGACAAGCTTATTATAATTCAGCTCCGGTGTGGTTAACCGGAGCTGAAAATCTCGCCCTCTCAGCTTGTGAGAGGGCGAGACAAGGTCAAGAACATGGTATACAGACGCTGGCGTGTATACCATCGGCTGACGGTGTTTTGGAGCTTGGATCTACTGAGTTGATTTATCAGAATAATGATTTGATGAATAAAGTGAAGATGCTTTTCAATTTTAACAATAATTTTGATTTTGGATCTTCTTGGCAGTTAGGTAATTCTGCCCATCATGGTGAGAATGATCCTTCTTCGATTTGGATTAACGATCCTGAAGCTAGAGATTCTGTTGATAATAATTCTCTTGTTACAACAACAACAACGGCAGCAACAAATGCTTCAATTTCAATTCCAAGTCACCAGCAGCACCAACAACAGAACAATAACAATAATCAGGGTTTGAGTGTGAGTGTGACGAAGACGATGCAATTTGAAACTCCTGGTTCGAGTACTTTAACGGAGGTTCCTAGTGTTGTTCATGTTTCAAGCAAGCAGAATAATCAAAGTTTCTTTTCTAAGGAAATGAATCTTTCTGATTATGGTGGTGGTAACAATCAGCAACGTTTGTTGAAGCCGGAATCCGGTGAGATTTTGTGTTTTGGTGAGAGTAAAAAGAGTTCTTATGTTGCTAATAATGGAAACTCGAATTCGAATTTCTTCTCTGGTCAATCACAGTTAGTTTCCGTTGCTGAGGAGAACAACAATGGGAACGGGAATGGGAATGGAAAGAGGAGATCACCGAATTCGAGGGGAAGCAATAATGATGATGGAATGCTATCTTTTACTTCTGGTGTAATTGTTCCACCAGGGGCGTCGAATTTGAAATTCTCTGGTGGTACTGGTGGTGGTGATTCGGACCATTCGGATCTGGAGGCTTCGGTGGTGAAGGAGGTGGATAGTAGTCGTGTGGTGGAGCCCGAAAAGAAGCCGAGGAAGAGAGGGAGGAAGCCGGCAAACGGAAGAGAGGAACCGTTGAATCATGTTGAAGCTGAGAGGCAAAGAAGAGAGAAGCTGAATCAAAGATTCTATGCACTTCGAGCAGTTGTTCCTAATGTTTCAAAGATGGATAAAGCTTCACTTTTGGGTGATGCTATATCTTACATTACTGAGTTGAAAACAAAGCTTCAGAAAACTGAATCCAATAAAGATGGATTAGAAAAGCAACTTGATGCGATGAAGAATGAGATTCAGAAAATCAACGAAAACCAGTCTCATCAGCCACCACAACAGCAGCAACAACAACAGCCAGTACCAAATAAACCCTCTTCGAATCAAGCTCTAATCGATTTAGATATCGATGTTAAGATTATAGGCTGGGATGCAATGATAAGGGTCCAATGCAGTAAGAAAAACCACCCGGCAGCAAGGTTAATGGCAGCGTTAATGGAGCTTGACCTAGAAGTGCACCATGCCAGTGTGTCTGTGGTAAATGATTTGATGATACAACAAGCAACCGTGAAAATGGGGAGTCGTTTTTACACTCAGGAGCAGCTTCGAGCGGCATTGTCCTCTAAAGTCGGGGATGTTCAATAA
Protein sequence

>MsaT026195.1

MNLWSDDNSSVMEAFMTSSDLSTLWPPAPPSQPPQTTTGFNQDTLQQRLQALIEGAKEVWTYAIFWQPSYDYSGSSLLGWGDGYYKGEEDKTKAKKSKVTSPAEQEHRRKVLRELNSLISGNPVTEESPVDEEVTDTEWFFLVSMTQSFVNGTGLPGQAYYNSAPVWLTGAENLALSACERARQGQEHGIQTLACIPSADGVLELGSTELIYQNNDLMNKVKMLFNFNNNFDFGSSWQLGNSAHHGENDPSSIWINDPEARDSVDNNSLVTTTTTAATNASISIPSHQQHQQQNNNNNQGLSVSVTKTMQFETPGSSTLTEVPSVVHVSSKQNNQSFFSKEMNLSDYGGGNNQQRLLKPESGEILCFGESKKSSYVANNGNSNSNFFSGQSQLVSVAEENNNGNGNGNGKRRSPNSRGSNNDDGMLSFTSGVIVPPGASNLKFSGGTGGGDSDHSDLEASVVKEVDSSRVVEPEKKPRKRGRKPANGREEPLNHVEAERQRREKLNQRFYALRAVVPNVSKMDKASLLGDAISYITELKTKLQKTESNKDGLEKQLDAMKNEIQKINENQSHQPPQQQQQQQPVPNKPSSNQALIDLDIDVKIIGWDAMIRVQCSKKNHPAARLMAALMELDLEVHHASVSVVNDLMIQQATVKMGSRFYTQEQLRAALSSKVGDVQ*