AlfalfaGEDB | MsaG026335

M. sativa ssp. caerulea / MsaG026335

KEGG: -
GO: -
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026335	XP_024640069.1	96.279	215	7	1	1	215	1	214	4.32e-148	420

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026335	sp\|P0CB25\|BH131_ARATH	46.154	195	88	5	4	191	38	222	6.02e-47	158

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026335	tr\|A0A396HSP5\|A0A396HSP5_MEDTR	96.154	208	7	1	8	215	1	207	3.80e-143	407

TFs/TRs:

Gene ID	Type	Classification
MsaG026335	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG006633	MsaG026335	0.801516	1.454972e-48	2.035737e-46
MsaG009178	MsaG026335	0.801063	1.799201e-48	2.491627e-46
MsaG009532	MsaG026335	0.807644	7.769067e-50	1.253509e-47
MsaG011525	MsaG026335	0.806774	1.185229e-49	1.873328e-47
MsaG013156	MsaG026335	0.805214	2.514504e-49	3.831128e-47
MsaG013970	MsaG026335	0.805182	2.553242e-49	3.887307e-47
MsaG014484	MsaG026335	0.811774	1.015863e-50	1.811675e-48
MsaG021783	MsaG026335	0.810315	2.096460e-50	3.607751e-48
MsaG021890	MsaG026335	0.805391	2.309045e-49	3.532587e-47
MsaG024417	MsaG026335	0.816589	8.884930e-52	1.788287e-49
MsaG026335	MsaG026336	0.831324	3.210997e-55	9.652760e-53
MsaG026335	MsaG026465	0.801226	1.666907e-48	2.316939e-46
MsaG026335	MsaG027991	0.800389	2.465482e-48	3.362942e-46
MsaG026335	MsaG028417	0.807685	7.614466e-50	1.229812e-47
MsaG026335	MsaG029311	0.819442	2.027348e-52	4.393586e-50
MsaG026335	MsaG032634	0.811623	1.094946e-50	1.945512e-48
MsaG026335	MsaG032860	0.807156	9.848181e-50	1.570597e-47
MsaG026335	MsaG033563	0.800935	1.910321e-48	2.637924e-46
MsaG026335	MsaG041495	0.801973	1.173400e-48	1.658941e-46
MsaG026335	MsaG042575	0.812259	7.972927e-51	1.438946e-48
MsaG026335	MsaG042732	0.801196	1.690407e-48	2.348049e-46
MsaG026335	MsaG042799	0.806140	1.610272e-49	2.507228e-47
MsaG026335	MsaG044874	0.805232	2.492903e-49	3.799813e-47
MsaG026335	MsaG009150	0.834599	4.968670e-56	1.644532e-53
MsaG026335	MsaG013105	0.803304	6.254457e-49	9.116644e-47
MsaG026335	MsaG019154	0.807986	6.577814e-50	1.070106e-47
MsaG026335	MsaG032520	0.809694	2.848011e-50	4.827752e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026335	MtrunA17_Chr5g0412371	96.154	208	7	1	8	215	1	207	7.33e-147	407
MsaG026335	MtrunA17_Chr7g0255961	33.473	239	118	8	3	207	1	232	2.84e-26	101
MsaG026335	MtrunA17_Chr1g0198801	37.126	167	87	3	50	205	156	315	2.02e-25	101
MsaG026335	MtrunA17_Chr5g0427361	40.373	161	75	2	52	191	37	197	8.02e-25	97.8
MsaG026335	MtrunA17_Chr3g0113721	35.928	167	91	3	52	205	39	202	5.95e-24	95.1
MsaG026335	MtrunA17_Chr2g0325521	39.583	144	72	3	47	184	54	188	1.61e-20	86.3
MsaG026335	MtrunA17_Chr4g0041051	35.220	159	86	3	41	192	57	205	3.29e-18	80.5
MsaG026335	MtrunA17_Chr4g0045901	36.364	143	78	4	46	182	68	203	9.74e-12	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026335	AT4G38070.1	46.154	195	88	5	4	191	38	222	6.12e-48	158
MsaG026335	AT3G25710.1	43.478	138	68	3	50	184	130	260	4.34e-26	103
MsaG026335	AT1G68810.1	42.012	169	78	3	50	205	172	333	2.12e-25	102
MsaG026335	AT3G56770.1	33.908	174	92	6	47	207	39	202	6.37e-23	92.8
MsaG026335	AT2G41130.1	36.416	173	92	5	46	207	60	225	2.78e-20	86.3
MsaG026335	AT2G40200.1	38.235	136	66	4	53	179	64	190	4.37e-20	85.9

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGGGCGGACAAATTGAATT+TGG	0.240537	5:-20538857	MsaT026335.1:CDS
TGTCCGCCCCACTAGGAAAC+TGG	0.364856	5:+20538869	None:intergenic
TTGGACCCATAACACACTTC+TGG	0.378699	5:+20538690	None:intergenic
ACACTAACTTTGATCAAGTT+GGG	0.389149	5:+20539268	None:intergenic
AGGTGTTGAAAATGAAGGTT+TGG	0.393224	5:-20538668	MsaT026335.1:CDS
AATGAAAGCCAAAGTTGTTA+AGG	0.399548	5:-20538740	MsaT026335.1:CDS
GAAGAACCAGAAGTGTGTTA+TGG	0.408049	5:-20538696	MsaT026335.1:CDS
CATCTCAACCTTAACAACTT+TGG	0.408942	5:+20538732	None:intergenic
AAGAACCAGAAGTGTGTTAT+GGG	0.427563	5:-20538695	MsaT026335.1:CDS
AAAGGCAGAGAACAAATTAC+TGG	0.428363	5:-20539371	MsaT026335.1:CDS
GACACTAACTTTGATCAAGT+TGG	0.443559	5:+20539267	None:intergenic
GATGAGGCGGTATACTCAAT+AGG	0.446628	5:-20538596	None:intergenic
GGTGTTGAAAATGAAGGTTT+GGG	0.450241	5:-20538667	MsaT026335.1:CDS
GATATAGATGACATTAATCC+TGG	0.463604	5:+20538778	None:intergenic
TACCGCCTCATCTTAAAGCT+TGG	0.493542	5:+20538607	None:intergenic
TTGAGTTGTGAGGATAGGCC+AGG	0.504463	5:-20538796	MsaT026335.1:CDS
TTCAATTTGTCCGCCCCACT+AGG	0.507536	5:+20538862	None:intergenic
AGACGTAGAATGCGAATCAA+TGG	0.510341	5:-20539319	MsaT026335.1:CDS
CATGTTCGAAATTACTATAA+CGG	0.518424	5:-20539475	MsaT026335.1:CDS
GTTTGCGAGATGAATGAACT+TGG	0.518532	5:+20539424	None:intergenic
CAAAGTTGTTAAGGTTGAGA+TGG	0.542500	5:-20538731	MsaT026335.1:CDS
GTCCAAGGTGTTGAAAATGA+AGG	0.548376	5:-20538673	MsaT026335.1:CDS
AGGAAACTGGATCTCCTTTG+AGG	0.551448	5:+20538882	None:intergenic
CTACATTGAGTTGTGAGGAT+AGG	0.570986	5:-20538801	MsaT026335.1:CDS
GGAAAAGTGTAATGATGAGG+AGG	0.576454	5:-20538836	MsaT026335.1:CDS
AGAAGTGTGTTATGGGTCCA+AGG	0.587934	5:-20538688	MsaT026335.1:CDS
AGGAGATCCAGTTTCCTAGT+GGG	0.589469	5:-20538876	MsaT026335.1:CDS
CACTAACTTTGATCAAGTTG+GGG	0.594450	5:+20539269	None:intergenic
GTTGAGATGGTGACAGTGGG+TGG	0.609342	5:-20538718	MsaT026335.1:CDS
GAAAAGTGTAATGATGAGGA+GGG	0.610839	5:-20538835	MsaT026335.1:CDS
GGAGACTATCAAGCAAGTGA+AGG	0.617469	5:-20538950	MsaT026335.1:CDS
TAAGGTTGAGATGGTGACAG+TGG	0.619246	5:-20538722	MsaT026335.1:CDS
AGATAGCCATGGTACCTCAA+AGG	0.623032	5:-20538896	MsaT026335.1:CDS
TAACTTTGATCAAGTTGGGG+AGG	0.625634	5:+20539272	None:intergenic
TTTCTCGAAGCAAAGATCAA+AGG	0.627837	5:-20539389	MsaT026335.1:CDS
GGACAAGGCATCAATACTAG+TGG	0.628919	5:-20538971	MsaT026335.1:CDS
GGATCTCCTTTGAGGTACCA+TGG	0.636586	5:+20538890	None:intergenic
AAGGTTGAGATGGTGACAGT+GGG	0.645311	5:-20538721	MsaT026335.1:CDS
GGAGATCCAGTTTCCTAGTG+GGG	0.653099	5:-20538875	MsaT026335.1:CDS
AAGGAGATCCAGTTTCCTAG+TGG	0.655156	5:-20538877	MsaT026335.1:CDS
AGCCAAGCTTTAAGATGAGG+CGG	0.655183	5:-20538609	MsaT026335.1:CDS
AGTTGGGGAGGATACTGCGG+AGG	0.655539	5:+20539284	None:intergenic
ATAAGCCAAGCTTTAAGATG+AGG	0.659617	5:-20538612	MsaT026335.1:CDS
AAACTAGAAGAAGATAGCCA+TGG	0.662686	5:-20538907	MsaT026335.1:CDS
TATTGATTAACAGAAGGACA+AGG	0.666615	5:-20538986	MsaT026335.1:intron
GATCCAGTTTCCTAGTGGGG+CGG	0.671647	5:-20538872	MsaT026335.1:CDS
TTTGGAAAAGTGTAATGATG+AGG	0.678742	5:-20538839	MsaT026335.1:CDS
GAAAGCTACATTGAGTTGTG+AGG	0.689461	5:-20538806	MsaT026335.1:CDS
TCAAGTTGGGGAGGATACTG+CGG	0.696028	5:+20539281	None:intergenic
GTTGGGGAGGATACTGCGGA+GGG	0.717546	5:+20539285	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	20538597	20539602	20538597	ID=MsaG026335
Chr5	mRNA	20538597	20539602	20538597	ID=MsaT026335.1;Parent=MsaG026335
Chr5	exon	20538597	20538995	20538597	ID=MsaT026335.1.exon3;Parent=MsaT026335.1
Chr5	CDS	20538597	20538995	20538597	ID=cds.MsaT026335.1;Parent=MsaT026335.1
Chr5	exon	20539276	20539506	20539276	ID=MsaT026335.1.exon2;Parent=MsaT026335.1
Chr5	CDS	20539276	20539506	20539276	ID=cds.MsaT026335.1;Parent=MsaT026335.1
Chr5	exon	20539585	20539602	20539585	ID=MsaT026335.1.exon1;Parent=MsaT026335.1
Chr5	CDS	20539585	20539602	20539585	ID=cds.MsaT026335.1;Parent=MsaT026335.1

Gene Sequence

>MsaT026335.1

ATGGTTTTACTTCGACAGGGCATGCAGCATGTTCGAAATTACTATAACGGAGAAGCTCAAATGCTAAGAAGCAACTTTCCAAGTTCATTCATCTCGCAAACATTCAAAAGCAGTTTCTCGAAGCAAAGATCAAAGGCAGAGAACAAATTACTGGCAGCTAAGAAACATAGTGAAGCAGAAAAGAGACGTAGAATGCGAATCAATGGTCAATATGACACCCTCCGCAGTATCCTCCCCAACTTGATCAAAAAGGACAAGGCATCAATACTAGTGGAGACTATCAAGCAAGTGAAGGAGTTGAAGAAAAAAGCATCAAAACTAGAAGAAGATAGCCATGGTACCTCAAAGGAGATCCAGTTTCCTAGTGGGGCGGACAAATTGAATTTGGAAAAGTGTAATGATGAGGAGGGTCTAGTGAAAGCTACATTGAGTTGTGAGGATAGGCCAGGATTAATGTCATCTATATCGAGAGCACTATTGTCAATGAAAGCCAAAGTTGTTAAGGTTGAGATGGTGACAGTGGGTGGAAGAACCAGAAGTGTGTTATGGGTCCAAGGTGTTGAAAATGAAGGTTTGGGAATGCTCAAGAGTACTTTGAAAGTTGTTATGCATAAGCCAAGCTTTAAGATGAGGCGGTATACTCAATAG

Protein sequence

>MsaT026335.1

MVLLRQGMQHVRNYYNGEAQMLRSNFPSSFISQTFKSSFSKQRSKAENKLLAAKKHSEAEKRRRMRINGQYDTLRSILPNLIKKDKASILVETIKQVKELKKKASKLEEDSHGTSKEIQFPSGADKLNLEKCNDEEGLVKATLSCEDRPGLMSSISRALLSMKAKVVKVEMVTVGGRTRSVLWVQGVENEGLGMLKSTLKVVMHKPSFKMRRYTQ*