Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026435 | AES96408.1 | 93.023 | 86 | 6 | 0 | 1 | 86 | 1 | 86 | 2.94e-52 | 168 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026435 | tr|G7K6X6|G7K6X6_MEDTR | 93.023 | 86 | 6 | 0 | 1 | 86 | 1 | 86 | 1.41e-52 | 168 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG026435 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026435 | MtrunA17_Chr5g0415081 | 93.023 | 86 | 6 | 0 | 1 | 86 | 1 | 86 | 2.71e-56 | 168 |
| MsaG026435 | MtrunA17_Chr5g0415071 | 59.091 | 66 | 25 | 1 | 1 | 66 | 1 | 64 | 8.36e-22 | 80.5 |
| MsaG026435 | MtrunA17_Chr7g0218881 | 50.000 | 78 | 36 | 2 | 6 | 82 | 1 | 76 | 1.26e-19 | 75.5 |
| MsaG026435 | MtrunA17_Chr6g0452021 | 46.377 | 69 | 35 | 1 | 16 | 84 | 123 | 189 | 3.45e-14 | 65.9 |
| MsaG026435 | MtrunA17_Chr6g0452001 | 43.056 | 72 | 39 | 1 | 13 | 84 | 142 | 211 | 2.24e-13 | 63.5 |
| MsaG026435 | MtrunA17_Chr5g0415031 | 42.029 | 69 | 38 | 1 | 16 | 84 | 125 | 191 | 8.50e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026435 | AT3G01320.2 | 48.387 | 62 | 30 | 1 | 23 | 84 | 60 | 119 | 1.37e-11 | 58.9 |
| MsaG026435 | AT3G01320.2 | 38.889 | 72 | 42 | 1 | 13 | 84 | 135 | 204 | 3.67e-11 | 57.8 |
| MsaG026435 | AT3G01320.1 | 48.387 | 62 | 30 | 1 | 23 | 84 | 60 | 119 | 1.45e-11 | 58.9 |
| MsaG026435 | AT3G01320.1 | 38.889 | 72 | 42 | 1 | 13 | 84 | 135 | 204 | 3.78e-11 | 57.8 |
| MsaG026435 | AT5G15020.1 | 40.278 | 72 | 41 | 1 | 13 | 84 | 129 | 198 | 2.21e-11 | 58.5 |
| MsaG026435 | AT5G15020.2 | 40.278 | 72 | 41 | 1 | 13 | 84 | 129 | 198 | 2.28e-11 | 58.5 |
Find 15 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGCTATGTGCGCGAGGTGA+AGG | 0.474033 | 5:-22009711 | MsaT026435.1:intron |
| GGCCATGATGACCTCATTGA+TGG | 0.490730 | 5:-22009001 | MsaT026435.1:CDS |
| TGAAAAGAATTTACGCTGAC+CGG | 0.518590 | 5:-22009760 | MsaT026435.1:CDS |
| ACCCATCAATGAGGTCATCA+TGG | 0.540419 | 5:+22008999 | None:intergenic |
| TTACGCTGACCGGCCAGTGG+TGG | 0.569040 | 5:-22009750 | MsaT026435.1:CDS |
| TGTTGGTAATGTTATTAGTG+AGG | 0.580980 | 5:-22009362 | MsaT026435.1:intron |
| ATAGAAAGAGAAAAGACTGT+TGG | 0.583807 | 5:-22009379 | MsaT026435.1:CDS |
| GCCATGATGACCTCATTGAT+GGG | 0.591592 | 5:-22009000 | MsaT026435.1:CDS |
| ACTGATAATCCACCACTGGC+CGG | 0.602964 | 5:+22009741 | None:intergenic |
| ATATACTGATAATCCACCAC+TGG | 0.603993 | 5:+22009737 | None:intergenic |
| AATTTACGCTGACCGGCCAG+TGG | 0.644697 | 5:-22009753 | MsaT026435.1:CDS |
| ACAAATTTCATTCCTCTACG+TGG | 0.649296 | 5:-22008974 | MsaT026435.1:CDS |
| CATATACACTAGCCACGTAG+AGG | 0.658315 | 5:+22008962 | None:intergenic |
| AATTTGTAAACCCATCAATG+AGG | 0.691671 | 5:+22008990 | None:intergenic |
| TATTTCGAGCTATGTGCGCG+AGG | 0.697429 | 5:-22009717 | MsaT026435.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 22008970 | 22009798 | 22008970 | ID=MsaG026435 |
| Chr5 | mRNA | 22008970 | 22009798 | 22008970 | ID=MsaT026435.1;Parent=MsaG026435 |
| Chr5 | exon | 22008970 | 22009041 | 22008970 | ID=MsaT026435.1.exon3;Parent=MsaT026435.1 |
| Chr5 | CDS | 22008970 | 22009041 | 22008970 | ID=cds.MsaT026435.1;Parent=MsaT026435.1 |
| Chr5 | exon | 22009363 | 22009467 | 22009363 | ID=MsaT026435.1.exon2;Parent=MsaT026435.1 |
| Chr5 | CDS | 22009363 | 22009467 | 22009363 | ID=cds.MsaT026435.1;Parent=MsaT026435.1 |
| Chr5 | exon | 22009712 | 22009798 | 22009712 | ID=MsaT026435.1.exon1;Parent=MsaT026435.1 |
| Chr5 | CDS | 22009712 | 22009798 | 22009712 | ID=cds.MsaT026435.1;Parent=MsaT026435.1 |
| Gene Sequence |
| Protein sequence |
