Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026436 | AES96405.2 | 69.412 | 85 | 26 | 0 | 1 | 85 | 1 | 85 | 6.62e-36 | 126 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026436 | sp|Q9XIK6|SNL6_ARATH | 41.791 | 67 | 39 | 0 | 16 | 82 | 164 | 230 | 4.56e-14 | 68.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026436 | tr|G7K6X3|G7K6X3_MEDTR | 69.412 | 85 | 26 | 0 | 1 | 85 | 1 | 85 | 3.16e-36 | 126 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG026436 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026436 | MtrunA17_Chr5g0415081 | 63.095 | 84 | 29 | 1 | 1 | 82 | 1 | 84 | 6.86e-33 | 108 |
| MsaG026436 | MtrunA17_Chr5g0415071 | 71.875 | 64 | 18 | 0 | 1 | 64 | 1 | 64 | 1.04e-28 | 97.8 |
| MsaG026436 | MtrunA17_Chr7g0218881 | 54.930 | 71 | 31 | 1 | 6 | 75 | 1 | 71 | 1.38e-18 | 72.8 |
| MsaG026436 | MtrunA17_Chr6g0452021 | 39.759 | 83 | 42 | 1 | 3 | 85 | 118 | 192 | 2.73e-15 | 68.9 |
| MsaG026436 | MtrunA17_Chr6g0452001 | 41.429 | 70 | 41 | 0 | 13 | 82 | 142 | 211 | 5.18e-15 | 68.2 |
| MsaG026436 | MtrunA17_Chr5g0415031 | 38.554 | 83 | 44 | 1 | 2 | 84 | 118 | 193 | 9.29e-14 | 64.7 |
| MsaG026436 | MtrunA17_Chr8g0364231 | 40.299 | 67 | 40 | 0 | 16 | 82 | 124 | 190 | 2.05e-11 | 58.2 |
| MsaG026436 | MtrunA17_Chr7g0218921 | 50.847 | 59 | 28 | 1 | 28 | 85 | 1 | 59 | 3.33e-11 | 53.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026436 | AT1G10450.3 | 41.791 | 67 | 39 | 0 | 16 | 82 | 164 | 230 | 4.64e-15 | 68.9 |
| MsaG026436 | AT1G10450.2 | 41.791 | 67 | 39 | 0 | 16 | 82 | 113 | 179 | 5.21e-15 | 68.6 |
| MsaG026436 | AT1G10450.1 | 41.791 | 67 | 39 | 0 | 16 | 82 | 113 | 179 | 5.21e-15 | 68.6 |
| MsaG026436 | AT3G01320.1 | 40.000 | 70 | 42 | 0 | 13 | 82 | 135 | 204 | 1.35e-13 | 64.7 |
| MsaG026436 | AT3G01320.2 | 40.000 | 70 | 42 | 0 | 13 | 82 | 135 | 204 | 1.41e-13 | 64.7 |
| MsaG026436 | AT5G15020.2 | 41.429 | 70 | 41 | 0 | 13 | 82 | 129 | 198 | 3.75e-13 | 63.5 |
| MsaG026436 | AT1G70060.2 | 37.805 | 82 | 50 | 1 | 1 | 82 | 83 | 163 | 3.93e-13 | 63.2 |
| MsaG026436 | AT1G70060.1 | 37.805 | 82 | 50 | 1 | 1 | 82 | 83 | 163 | 3.93e-13 | 63.2 |
| MsaG026436 | AT5G15020.1 | 41.429 | 70 | 41 | 0 | 13 | 82 | 129 | 198 | 4.09e-13 | 63.2 |
| MsaG026436 | AT1G24190.3 | 43.284 | 67 | 38 | 0 | 16 | 82 | 96 | 162 | 1.23e-12 | 62.0 |
| MsaG026436 | AT1G24190.2 | 43.284 | 67 | 38 | 0 | 16 | 82 | 96 | 162 | 1.47e-12 | 61.6 |
| MsaG026436 | AT1G24190.1 | 43.284 | 67 | 38 | 0 | 16 | 82 | 96 | 162 | 1.47e-12 | 61.6 |
| MsaG026436 | AT1G59890.4 | 37.313 | 67 | 42 | 0 | 16 | 82 | 125 | 191 | 5.14e-12 | 60.1 |
| MsaG026436 | AT1G59890.3 | 37.313 | 67 | 42 | 0 | 16 | 82 | 125 | 191 | 5.25e-12 | 60.1 |
| MsaG026436 | AT1G59890.1 | 37.313 | 67 | 42 | 0 | 16 | 82 | 125 | 191 | 5.25e-12 | 60.1 |
| MsaG026436 | AT1G59890.2 | 37.313 | 67 | 42 | 0 | 16 | 82 | 125 | 191 | 5.25e-12 | 60.1 |
| MsaG026436 | AT1G70030.1 | 36.000 | 75 | 47 | 1 | 11 | 85 | 87 | 160 | 5.09e-11 | 55.5 |
Find 18 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGTGGTGGATTATGAATA+TGG | 0.290334 | 5:-22040983 | MsaT026436.1:CDS |
| AGAGTCTTGATGATGTCATT+TGG | 0.357491 | 5:-22040754 | MsaT026436.1:CDS |
| GAGTCTTGATGATGTCATTT+GGG | 0.414208 | 5:-22040753 | MsaT026436.1:CDS |
| GGCCATGATGATCTTATTGA+TGG | 0.460349 | 5:-22040611 | MsaT026436.1:CDS |
| GCCATGATGATCTTATTGAT+GGG | 0.565009 | 5:-22040610 | MsaT026436.1:CDS |
| GAGATATATAATGAAGGTGA+AGG | 0.576899 | 5:-22040957 | MsaT026436.1:intron |
| TTTATTGATTTCCTTCCGCA+AGG | 0.586502 | 5:-22040587 | MsaT026436.1:CDS |
| ATTCATAATCCACCACTGGC+CGG | 0.587375 | 5:+22040987 | None:intergenic |
| TGAAAAGAACATATGCTGAC+CGG | 0.588434 | 5:-22041006 | MsaT026436.1:CDS |
| ATATGCTGACCGGCCAGTGG+TGG | 0.590440 | 5:-22040996 | MsaT026436.1:CDS |
| ATCACTAGCCACCTTGCGGA+AGG | 0.593474 | 5:+22040576 | None:intergenic |
| TGGTTTGAGATATATAATGA+AGG | 0.601721 | 5:-22040963 | MsaT026436.1:CDS |
| ACCCATCAATAAGATCATCA+TGG | 0.602513 | 5:+22040609 | None:intergenic |
| CCATATTCATAATCCACCAC+TGG | 0.614448 | 5:+22040983 | None:intergenic |
| AACATATGCTGACCGGCCAG+TGG | 0.637326 | 5:-22040999 | MsaT026436.1:CDS |
| ATTGATTTCCTTCCGCAAGG+TGG | 0.643769 | 5:-22040584 | MsaT026436.1:CDS |
| TCTTGATGATGTCATTTGGG+CGG | 0.663888 | 5:-22040750 | MsaT026436.1:intron |
| ATATATCACTAGCCACCTTG+CGG | 0.674598 | 5:+22040572 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 22040580 | 22041044 | 22040580 | ID=MsaG026436 |
| Chr5 | mRNA | 22040580 | 22041044 | 22040580 | ID=MsaT026436.1;Parent=MsaG026436 |
| Chr5 | exon | 22040580 | 22040651 | 22040580 | ID=MsaT026436.1.exon3;Parent=MsaT026436.1 |
| Chr5 | CDS | 22040580 | 22040651 | 22040580 | ID=cds.MsaT026436.1;Parent=MsaT026436.1 |
| Chr5 | exon | 22040751 | 22040849 | 22040751 | ID=MsaT026436.1.exon2;Parent=MsaT026436.1 |
| Chr5 | CDS | 22040751 | 22040849 | 22040751 | ID=cds.MsaT026436.1;Parent=MsaT026436.1 |
| Chr5 | exon | 22040958 | 22041044 | 22040958 | ID=MsaT026436.1.exon1;Parent=MsaT026436.1 |
| Chr5 | CDS | 22040958 | 22041044 | 22040958 | ID=cds.MsaT026436.1;Parent=MsaT026436.1 |
| Gene Sequence |
| Protein sequence |
