AlfalfaGEDB | MsaG026437

M. sativa ssp. caerulea / MsaG026437

KEGG: K11644
GO: -
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026437	AES96405.2	77.586	58	13	0	96	153	28	85	7.49e-23	96.7
MsaG026437	AES96405.2	82.353	85	15	0	1	85	1	85	3.21e-42	145

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026437	sp\|Q9LFQ3\|SNL2_ARATH	40.000	145	67	4	24	150	56	198	8.65e-22	94.0
MsaG026437	sp\|Q9LFQ3\|SNL2_ARATH	47.887	71	37	0	12	82	128	198	1.05e-13	70.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026437	tr\|G7K6X3\|G7K6X3_MEDTR	77.586	58	13	0	96	153	28	85	3.58e-23	96.7
MsaG026437	tr\|G7K6X3\|G7K6X3_MEDTR	82.353	85	15	0	1	85	1	85	1.53e-42	145

TFs/TRs:

Gene ID	Type	Classification
MsaG026437	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026437	MtrunA17_Chr5g0415081	69.767	86	24	1	1	84	1	86	5.60e-36	119
MsaG026437	MtrunA17_Chr5g0415081	58.904	73	28	1	82	152	14	86	1.22e-19	78.2
MsaG026437	MtrunA17_Chr5g0415071	77.778	72	12	1	1	72	1	68	7.83e-34	113
MsaG026437	MtrunA17_Chr5g0415071	68.889	45	10	1	96	140	28	68	2.01e-14	64.3
MsaG026437	MtrunA17_Chr6g0452001	40.136	147	66	4	24	150	67	211	1.16e-23	95.9
MsaG026437	MtrunA17_Chr6g0452001	50.000	70	35	0	13	82	142	211	7.33e-16	73.6
MsaG026437	MtrunA17_Chr5g0415031	39.456	147	67	4	24	150	47	191	1.29e-22	93.2
MsaG026437	MtrunA17_Chr5g0415031	50.746	67	33	0	16	82	125	191	2.23e-14	69.3
MsaG026437	MtrunA17_Chr6g0452021	37.063	143	74	3	24	150	47	189	1.68e-21	89.4
MsaG026437	MtrunA17_Chr6g0452021	49.254	67	34	0	16	82	123	189	8.41e-15	70.5
MsaG026437	MtrunA17_Chr8g0364231	36.301	146	73	3	24	150	46	190	1.83e-21	89.7
MsaG026437	MtrunA17_Chr8g0364231	44.776	67	37	0	16	82	124	190	2.77e-12	63.5
MsaG026437	MtrunA17_Chr7g0218881	55.263	76	33	1	6	80	1	76	2.30e-21	82.4
MsaG026437	MtrunA17_Chr1g0206771	29.710	138	86	2	24	150	20	157	1.62e-14	67.4
MsaG026437	MtrunA17_Chr7g0218921	54.237	59	26	1	96	153	1	59	2.29e-13	61.2
MsaG026437	MtrunA17_Chr7g0218921	50.847	59	28	1	28	85	1	59	4.70e-12	57.8
MsaG026437	MtrunA17_Chr7g0218901	49.091	55	28	0	96	150	1	55	1.35e-12	58.9
MsaG026437	MtrunA17_Chr7g0218901	45.455	55	30	0	28	82	1	55	2.05e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026437	AT5G15020.2	40.000	145	67	4	24	150	56	198	8.13e-23	94.0
MsaG026437	AT5G15020.2	47.887	71	37	0	12	82	128	198	1.01e-14	70.9
MsaG026437	AT5G15020.1	40.000	145	67	4	24	150	56	198	8.79e-23	94.0
MsaG026437	AT5G15020.1	47.887	71	37	0	12	82	128	198	1.06e-14	70.9
MsaG026437	AT3G01320.1	38.621	145	70	3	24	150	61	204	1.11e-22	93.6
MsaG026437	AT3G01320.1	38.000	100	53	1	13	112	135	225	1.26e-14	70.5
MsaG026437	AT3G01320.2	38.621	145	70	3	24	150	61	204	1.14e-22	93.6
MsaG026437	AT3G01320.2	38.000	100	53	1	13	112	135	225	1.29e-14	70.5
MsaG026437	AT1G24190.3	39.456	147	67	4	24	150	18	162	3.53e-22	92.0
MsaG026437	AT1G24190.3	50.746	67	33	0	16	82	96	162	2.10e-13	67.0
MsaG026437	AT1G70030.1	35.616	146	70	4	28	153	19	160	5.67e-22	86.7
MsaG026437	AT1G70030.1	39.744	78	46	1	8	85	84	160	1.05e-11	59.7
MsaG026437	AT1G70060.2	39.189	148	67	4	24	150	18	163	2.69e-21	89.7
MsaG026437	AT1G70060.2	50.000	72	36	0	11	82	92	163	5.51e-14	68.6
MsaG026437	AT1G70060.1	39.189	148	67	4	24	150	18	163	2.69e-21	89.7
MsaG026437	AT1G70060.1	50.000	72	36	0	11	82	92	163	5.51e-14	68.6
MsaG026437	AT1G24190.2	39.456	147	67	4	24	150	18	162	5.24e-21	89.0
MsaG026437	AT1G24190.2	50.746	67	33	0	16	82	96	162	8.34e-13	65.5
MsaG026437	AT1G24190.1	39.456	147	67	4	24	150	18	162	5.39e-21	89.0
MsaG026437	AT1G24190.1	50.746	67	33	0	16	82	96	162	8.58e-13	65.1
MsaG026437	AT1G10450.3	35.417	144	75	3	24	150	88	230	2.53e-20	87.0
MsaG026437	AT1G10450.3	49.254	67	34	0	16	82	164	230	4.01e-14	68.9
MsaG026437	AT1G10450.2	35.417	144	75	3	24	150	37	179	2.55e-20	87.0
MsaG026437	AT1G10450.2	49.254	67	34	0	16	82	113	179	4.03e-14	68.9
MsaG026437	AT1G10450.1	35.417	144	75	3	24	150	37	179	2.55e-20	87.0
MsaG026437	AT1G10450.1	49.254	67	34	0	16	82	113	179	4.03e-14	68.9
MsaG026437	AT1G59890.4	34.722	144	76	3	24	150	49	191	1.09e-18	82.0
MsaG026437	AT1G59890.4	43.284	67	38	0	16	82	125	191	3.61e-11	60.5
MsaG026437	AT1G59890.2	34.722	144	76	3	24	150	49	191	1.14e-18	82.0
MsaG026437	AT1G59890.2	43.284	67	38	0	16	82	125	191	3.58e-11	60.5
MsaG026437	AT1G59890.3	34.722	144	76	3	24	150	49	191	1.18e-18	82.0
MsaG026437	AT1G59890.3	43.284	67	38	0	16	82	125	191	3.61e-11	60.5
MsaG026437	AT1G59890.1	34.722	144	76	3	24	150	49	191	1.22e-18	82.0
MsaG026437	AT1G59890.1	43.284	67	38	0	16	82	125	191	3.72e-11	60.5
MsaG026437	AT1G24200.1	35.897	156	81	5	11	150	7	159	8.35e-18	76.6
MsaG026437	AT1G24200.1	47.887	71	34	1	24	91	101	171	2.89e-11	59.3
MsaG026437	AT1G24230.1	31.507	146	85	4	17	147	83	228	1.70e-15	71.2
MsaG026437	AT1G70030.2	29.252	147	87	3	7	153	10	139	7.29e-14	65.1
MsaG026437	AT1G23810.1	29.286	140	93	2	4	142	93	227	2.87e-11	59.7
MsaG026437	AT1G24250.1	30.496	141	92	2	3	142	103	238	3.22e-11	59.7

Find 21 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAAAGAATATACGCTAAC+CGG	0.465728	5:-22054767	MsaT026437.1:CDS
GGCCATGATGACCTCATTGA+TGG	0.490730	5:-22044341	MsaT026437.1:CDS
GGCCATGATGACCTCATTGA+TGG	0.490730	5:-22054299	MsaT026437.1:CDS
TGTTGCGAGATATGTGTTCA+AGG	0.537764	5:-22054724	MsaT026437.1:CDS
ACCCATCAATGAGGTCATCA+TGG	0.541672	5:+22044339	None:intergenic
ACCCATCAATGAGGTCATCA+TGG	0.541672	5:+22054297	None:intergenic
AAGAATATACGCTAACCGGC+CGG	0.564322	5:-22054763	MsaT026437.1:CDS
GCCATGATGACCTCATTGAT+GGG	0.591592	5:-22044340	MsaT026437.1:CDS
GCCATGATGACCTCATTGAT+GGG	0.591592	5:-22054298	MsaT026437.1:CDS
AATGATAATTCACCACCGGC+CGG	0.615597	5:+22054748	None:intergenic
GAGATATGTGTTCAAGGTGA+AGG	0.619178	5:-22054718	MsaT026437.1:intron
AGAAATTTCATTCCTCTACG+TGG	0.633354	5:-22044314	MsaT026437.1:CDS
AGAAATTTCATTCCTCTACG+TGG	0.633354	5:-22054272	MsaT026437.1:CDS
ACATAATGATAATTCACCAC+CGG	0.640520	5:+22054744	None:intergenic
AATATACGCTAACCGGCCGG+TGG	0.651380	5:-22054760	MsaT026437.1:CDS
CATATACACTAGCCACGTAG+AGG	0.658315	5:+22044302	None:intergenic
CATATACACTAGCCACGTAG+AGG	0.658315	5:+22054260	None:intergenic
AATTTCTAAACCCATCAATG+AGG	0.679105	5:+22044330	None:intergenic
AATTTCTAAACCCATCAATG+AGG	0.679105	5:+22054288	None:intergenic
TCTTGATGAAGTCATTCGCG+AGG	0.697148	5:-22044555	MsaT026437.1:intron
TCTTGATGAAGTCATTCGCG+AGG	0.697148	5:-22054513	MsaT026437.1:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	22044310	22054805	22044310	ID=MsaG026437
Chr5	mRNA	22044310	22054805	22044310	ID=MsaT026437.1;Parent=MsaG026437
Chr5	exon	22044310	22044381	22044310	ID=MsaT026437.1.exon5;Parent=MsaT026437.1
Chr5	CDS	22044310	22044381	22044310	ID=cds.MsaT026437.1;Parent=MsaT026437.1
Chr5	exon	22044556	22044688	22044556	ID=MsaT026437.1.exon4;Parent=MsaT026437.1
Chr5	CDS	22044556	22044688	22044556	ID=cds.MsaT026437.1;Parent=MsaT026437.1
Chr5	exon	22054269	22054339	22054269	ID=MsaT026437.1.exon3;Parent=MsaT026437.1
Chr5	CDS	22054269	22054339	22054269	ID=cds.MsaT026437.1;Parent=MsaT026437.1
Chr5	exon	22054514	22054612	22054514	ID=MsaT026437.1.exon2;Parent=MsaT026437.1
Chr5	CDS	22054514	22054612	22054514	ID=cds.MsaT026437.1;Parent=MsaT026437.1
Chr5	exon	22054719	22054805	22054719	ID=MsaT026437.1.exon1;Parent=MsaT026437.1
Chr5	CDS	22054719	22054805	22054719	ID=cds.MsaT026437.1;Parent=MsaT026437.1

Gene Sequence

>MsaT026437.1

ATGAAACCACATAAGATGAAAAGAATATACGCTAACCGGCCGGTGGTGAATTATCATTATGTTGCGAGATATGTGTTCAAGGTGAAGACTCGATTTCAACATGCTCGTCATGTGTATTCGTCGTTTCTAGACATACTGCGAATGTACAGAAAAAGAGAAAAAAGTCTTGATGAAGTCATTCGCGAGGTTGCTGAGCTTTTTGAAGGCCATGATGACCTCATTGATGGGTTTAGAAATTTCATTCCTCTACGTGGCTATTTTAATGAGTCTAATAGATCTTTTTTCATGCAGACTCGATTTCAACATGCTCGTCATGTGTATTCGTCGTTTCTAGACATACTGCGAATGTACAGAAAAAGAGAAAAAAGTCTTGATGAAGTCATTCGCGAGGTTGCTGAGCTTTTTGAAGGCCATGATGACCTCATTGATGGGTTTAGAAATTTCATTCCTCTACGTGGCTAG

Protein sequence

>MsaT026437.1

MKPHKMKRIYANRPVVNYHYVARYVFKVKTRFQHARHVYSSFLDILRMYRKREKSLDEVIREVAELFEGHDDLIDGFRNFIPLRGYFNESNRSFFMQTRFQHARHVYSSFLDILRMYRKREKSLDEVIREVAELFEGHDDLIDGFRNFIPLRG*