AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG026456

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026456 MtrunA17_Chr5g0414931 95.359 237 11 0 1 237 1 237 1.16e-139 391
MsaG026456 MtrunA17_Chr1g0170741 68.122 229 42 3 9 237 1 198 3.97e-88 259
MsaG026456 MtrunA17_Chr4g0059571 71.724 145 37 1 15 159 25 165 1.63e-65 202
MsaG026456 MtrunA17_Chr3g0118211 67.883 137 40 1 15 151 22 154 2.81e-60 188
MsaG026456 MtrunA17_Chr7g0256551 60.265 151 55 2 15 160 8 158 5.04e-60 189
MsaG026456 MtrunA17_Chr8g0352871 58.108 148 56 2 15 157 8 154 1.33e-53 170
MsaG026456 MtrunA17_Chr3g0082861 56.944 144 57 1 15 153 3 146 2.38e-53 169
MsaG026456 MtrunA17_Chr3g0128691 58.955 134 47 3 17 147 19 147 4.51e-50 160
MsaG026456 MtrunA17_Chr3g0124861 50.000 152 61 3 10 147 16 166 1.46e-45 152
MsaG026456 MtrunA17_Chr4g0021731 60.714 84 27 3 15 97 10 88 3.39e-25 95.1
MsaG026456 MtrunA17_Chr4g0030271 29.661 236 135 5 16 237 89 307 7.22e-20 88.2
MsaG026456 MtrunA17_Chr3g0127941 28.497 193 112 4 16 195 56 235 2.53e-17 80.5
MsaG026456 MtrunA17_Chr8g0345901 30.216 139 84 1 14 152 34 159 1.03e-14 72.8
MsaG026456 MtrunA17_Chr1g0181811 30.827 133 79 2 18 150 87 206 1.34e-14 72.8
MsaG026456 MtrunA17_Chr7g0276361 29.630 135 82 2 18 152 16 137 3.68e-13 68.6
MsaG026456 MtrunA17_Chr8g0376881 26.190 168 104 4 16 179 29 180 8.47e-13 67.4
MsaG026456 MtrunA17_Chr4g0074411 26.087 161 103 3 16 176 22 166 1.12e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026456 AT1G59940.1 55.022 229 63 5 11 229 28 226 3.39e-72 220
MsaG026456 AT1G59940.2 55.022 229 63 5 11 229 120 318 6.19e-71 220
MsaG026456 AT1G10470.3 54.331 254 56 7 11 233 15 239 2.30e-69 213
MsaG026456 AT1G10470.1 54.331 254 56 7 11 233 29 253 4.19e-69 213
MsaG026456 AT3G48100.1 71.223 139 37 2 16 154 25 160 2.27e-64 198
MsaG026456 AT5G62920.1 75.385 130 26 2 16 144 25 149 4.49e-63 195
MsaG026456 AT1G19050.1 65.035 143 46 1 9 151 17 155 1.51e-59 187
MsaG026456 AT1G74890.1 70.769 130 34 1 15 144 17 142 7.40e-59 185
MsaG026456 AT3G57040.2 59.310 145 53 1 15 153 8 152 2.27e-57 182
MsaG026456 AT3G57040.1 59.310 145 53 1 15 153 8 152 2.27e-57 182
MsaG026456 AT2G41310.1 59.864 147 55 2 15 157 8 154 3.00e-56 179
MsaG026456 AT3G56380.1 59.701 134 51 1 14 147 18 148 6.47e-56 176
MsaG026456 AT2G40670.1 60.000 135 51 2 14 147 27 159 9.41e-55 173
MsaG026456 AT3G56380.2 59.259 135 51 2 14 147 18 149 3.40e-54 171
MsaG026456 AT2G40670.2 59.559 136 51 3 14 147 27 160 4.66e-53 169
MsaG026456 AT1G10470.2 59.155 71 27 2 79 148 17 86 2.36e-21 88.2
MsaG026456 AT2G07440.1 55.000 60 27 0 98 157 9 68 3.50e-15 70.5
MsaG026456 AT2G46790.3 29.630 135 82 2 16 150 37 158 7.31e-15 73.6
MsaG026456 AT2G46790.1 29.630 135 82 2 16 150 37 158 9.64e-15 73.2
MsaG026456 AT5G60100.7 34.586 133 74 2 18 150 66 185 1.23e-14 72.8
MsaG026456 AT5G60100.6 34.586 133 74 2 18 150 66 185 1.23e-14 72.8
MsaG026456 AT5G60100.4 34.586 133 74 2 18 150 66 185 1.23e-14 72.8
MsaG026456 AT5G24470.1 30.657 137 80 3 16 152 52 173 1.67e-14 72.8
MsaG026456 AT5G60100.2 34.586 133 74 2 18 150 66 185 1.84e-14 72.4
MsaG026456 AT5G60100.5 34.586 133 74 2 18 150 66 185 2.03e-14 72.4
MsaG026456 AT5G60100.3 34.586 133 74 2 18 150 66 185 2.03e-14 72.4
MsaG026456 AT5G60100.1 34.586 133 74 2 18 150 66 185 2.03e-14 72.4
MsaG026456 AT5G02810.1 29.630 135 82 1 16 150 78 199 9.77e-14 70.5
MsaG026456 AT5G07210.2 32.576 132 72 3 15 146 15 129 1.17e-13 70.1

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAAGAGCACTACAATTTC+TGG 0.202396 5:-22626615 MsaT026456.1:CDS
TTCTCAAGAGGTTCATGTTT+TGG 0.250601 5:-22626902 MsaT026456.1:CDS
GTTTCCTTAGCCTGGTTTGA+AGG 0.364670 5:-22626461 MsaT026456.1:intron
CTCAAAATTTCAGCTTGTAA+AGG 0.419180 5:-22626835 MsaT026456.1:intron
AACTCATAACCAGTCATTCC+TGG 0.425647 5:+22626409 None:intergenic
AGCACTACAATTTCTGGGGT+TGG 0.454081 5:-22626609 MsaT026456.1:CDS
ATAAGAGCACTACAATTTCT+GGG 0.455946 5:-22626614 MsaT026456.1:CDS
TGAAAGTGTTGATGATGAAA+TGG 0.456331 5:+22625258 None:intergenic
TGAGTTGCTTAAGAAAATCA+AGG 0.460054 5:-22626392 MsaT026456.1:intron
ATTACAGATTATTGCATGCC+AGG 0.468259 5:-22626427 MsaT026456.1:CDS
TATTGCATGCCAGGAATGAC+TGG 0.472610 5:-22626418 MsaT026456.1:CDS
TTATCGTTACCAACACCGTT+GGG 0.475445 5:+22625361 None:intergenic
TCAGAAGACATGATCACTAC+TGG 0.477520 5:+22626248 None:intergenic
GGATAGGGATCAACAACAAA+AGG 0.483740 5:-22625321 MsaT026456.1:CDS
ACATCTTGCCACGCATTGAC+AGG 0.484895 5:-22626223 MsaT026456.1:intron
GAGCGATAACTTCACTGTCT+TGG 0.485735 5:+22625383 None:intergenic
GATGGTGATGGTGTAGGGAT+AGG 0.492736 5:-22625337 MsaT026456.1:CDS
TCCTTAGCCTGGTTTGAAGG+TGG 0.500088 5:-22626458 MsaT026456.1:intron
GCTTCCCGCTCTAACCTCCC+TGG 0.501953 5:+22625405 None:intergenic
ATCTGACGTGAAACGTTTGA+AGG 0.502511 5:-22625440 MsaT026456.1:CDS
TACTGGAATTGCTCTAAAAG+TGG 0.506655 5:+22626265 None:intergenic
GACAACCAGGGAGGTTAGAG+CGG 0.506833 5:-22625410 MsaT026456.1:CDS
ATGGTGATGGTGTAGGGATA+GGG 0.509422 5:-22625336 MsaT026456.1:CDS
ATTGGAGAATCAAGAATGTC+GGG 0.519018 5:+22625190 None:intergenic
TCCAAAGAGTCATTCGGTGC+CGG 0.528910 5:+22625150 None:intergenic
TTCTTGATTCTCCAATCAGA+CGG 0.540193 5:-22625183 MsaT026456.1:CDS
TTGATCGTAAAGTCATTGAA+AGG 0.541223 5:-22626861 MsaT026456.1:CDS
ATAACGATGGTGATGGTGTA+GGG 0.541250 5:-22625342 MsaT026456.1:CDS
TGACAATCCAAAGAGTCATT+CGG 0.543587 5:+22625144 None:intergenic
GATAACGATGGTGATGGTGT+AGG 0.548802 5:-22625343 MsaT026456.1:CDS
ATCAGACGGCTTAAAATGAC+CGG 0.557664 5:-22625169 MsaT026456.1:CDS
TTCACGTCAGATAATTTCAC+CGG 0.562688 5:+22625451 None:intergenic
GATTGGAGAATCAAGAATGT+CGG 0.564103 5:+22625189 None:intergenic
AAGAATGTCGGGTGAAGACA+AGG 0.567906 5:+22625201 None:intergenic
TATTGATGTTGATTCTCAAG+AGG 0.575024 5:-22626914 MsaT026456.1:CDS
GTTATCGTTACCAACACCGT+TGG 0.576665 5:+22625360 None:intergenic
GTTATCGCTCCCAACGGTGT+TGG 0.578920 5:-22625370 MsaT026456.1:CDS
GAAGGTGACGGTGATGATGA+TGG 0.581069 5:+22625232 None:intergenic
AGAGGATTTCATAGTGAAGC+CGG 0.581417 5:-22625470 MsaT026456.1:CDS
TCTGACGTGAAACGTTTGAA+GGG 0.584866 5:-22625439 MsaT026456.1:CDS
ACTAGATCCACCTTCAAACC+AGG 0.592739 5:+22626451 None:intergenic
GAAGACAAGGGTGATGATGA+AGG 0.594875 5:+22625214 None:intergenic
ACAACCAGGGAGGTTAGAGC+GGG 0.595105 5:-22625409 MsaT026456.1:CDS
TGAAGGGTTACATGACAACC+AGG 0.599838 5:-22625423 MsaT026456.1:CDS
TACTAGTACCTGTCAATGCG+TGG 0.604768 5:+22626215 None:intergenic
AAATGTAGATGCTTGGAGGA+AGG 0.613553 5:-22625496 MsaT026456.1:intron
TTAGTTACTGCAGTGGACAG+TGG 0.615929 5:-22626638 MsaT026456.1:intron
ATGCTTGGAGGAAGGTGCAG+AGG 0.640084 5:-22625488 MsaT026456.1:CDS
TAAGAGCACTACAATTTCTG+GGG 0.651134 5:-22626613 MsaT026456.1:CDS
AGTGAAGTTATCGCTCCCAA+CGG 0.653885 5:-22625376 MsaT026456.1:CDS
AGTGTTGATGATGAAATGGA+TGG 0.658494 5:+22625262 None:intergenic
GGTAACGATAACGATGGTGA+TGG 0.701040 5:-22625349 MsaT026456.1:CDS
AAGGGTGATGATGAAGGTGA+CGG 0.717497 5:+22625220 None:intergenic
GAAGGGTTACATGACAACCA+GGG 0.738936 5:-22625422 MsaT026456.1:CDS
AGAATGTCGGGTGAAGACAA+GGG 0.739456 5:+22625202 None:intergenic
GGTGTTGGTAACGATAACGA+TGG 0.744946 5:-22625355 MsaT026456.1:CDS
GGGTTACATGACAACCAGGG+AGG 0.751767 5:-22625419 MsaT026456.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 22625159 22626959 22625159 ID=MsaG026456
Chr5 mRNA 22625159 22626959 22625159 ID=MsaT026456.1;Parent=MsaG026456
Chr5 exon 22625159 22625510 22625159 ID=MsaT026456.1.exon5;Parent=MsaT026456.1
Chr5 CDS 22625159 22625510 22625159 ID=cds.MsaT026456.1;Parent=MsaT026456.1
Chr5 exon 22626224 22626294 22626224 ID=MsaT026456.1.exon4;Parent=MsaT026456.1
Chr5 CDS 22626224 22626294 22626224 ID=cds.MsaT026456.1;Parent=MsaT026456.1
Chr5 exon 22626393 22626473 22626393 ID=MsaT026456.1.exon3;Parent=MsaT026456.1
Chr5 CDS 22626393 22626473 22626393 ID=cds.MsaT026456.1;Parent=MsaT026456.1
Chr5 exon 22626571 22626656 22626571 ID=MsaT026456.1.exon2;Parent=MsaT026456.1
Chr5 CDS 22626571 22626656 22626571 ID=cds.MsaT026456.1;Parent=MsaT026456.1
Chr5 exon 22626836 22626959 22626836 ID=MsaT026456.1.exon1;Parent=MsaT026456.1
Chr5 CDS 22626836 22626959 22626836 ID=cds.MsaT026456.1;Parent=MsaT026456.1
Gene Sequence

>MsaT026456.1

ATGGAGACAAACAGTGTTGTTTCTATTGATGTTGATTCTCAAGAGGTTCATGTTTTGGCTGTTGATGATAGTCTTGTTGATCGTAAAGTCATTGAAAGGTTGCTCAAAATTTCAGCTTGTAAAGTTACTGCAGTGGACAGTGGAATAAGAGCACTACAATTTCTGGGGTTGGTTGATGAGAAAGAGACTTCTGAATCTGATGCTTTTGTTCCTGGTTTGAAGGTGGATCTAGTAATTACAGATTATTGCATGCCAGGAATGACTGGTTATGAGTTGCTTAAGAAAATCAAGGAATCATCCACTTTTAGAGCAATTCCAGTAGTGATCATGTCTTCTGAAAACATCTTGCCACGCATTGACAGATGCTTGGAGGAAGGTGCAGAGGATTTCATAGTGAAGCCGGTGAAATTATCTGACGTGAAACGTTTGAAGGGTTACATGACAACCAGGGAGGTTAGAGCGGGAAGCCAAGACAGTGAAGTTATCGCTCCCAACGGTGTTGGTAACGATAACGATGGTGATGGTGTAGGGATAGGGATCAACAACAAAAGGAAGCTAGACGAAGCATCTGATCTATCATCATCTGAACCATCCATTTCATCATCAACACTTTCATCACCATCATCATCACCGTCACCTTCATCATCACCCTTGTCTTCACCCGACATTCTTGATTCTCCAATCAGACGGCTTAAAATGACCGGCACCGAATGA
Protein sequence

>MsaT026456.1

METNSVVSIDVDSQEVHVLAVDDSLVDRKVIERLLKISACKVTAVDSGIRALQFLGLVDEKETSESDAFVPGLKVDLVITDYCMPGMTGYELLKKIKESSTFRAIPVVIMSSENILPRIDRCLEEGAEDFIVKPVKLSDVKRLKGYMTTREVRAGSQDSEVIAPNGVGNDNDGDGVGIGINNKRKLDEASDLSSSEPSISSSTLSSPSSSPSPSSSPLSSPDILDSPIRRLKMTGTE*