Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026540 | AES96260.1 | 94.975 | 199 | 10 | 0 | 1 | 199 | 101 | 299 | 3.77e-128 | 372 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026540 | tr|G7K2T4|G7K2T4_MEDTR | 94.975 | 199 | 10 | 0 | 1 | 199 | 101 | 299 | 1.80e-128 | 372 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG026540 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG007186 | MsaG026540 | 0.803180 | 6.632530e-49 | 9.640281e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026540 | MtrunA17_Chr5g0414031 | 94.975 | 199 | 10 | 0 | 1 | 199 | 132 | 330 | 1.52e-131 | 372 |
| MsaG026540 | MtrunA17_Chr5g0413991 | 89.655 | 87 | 9 | 0 | 113 | 199 | 20 | 106 | 3.77e-52 | 163 |
| MsaG026540 | MtrunA17_Chr5g0414011 | 91.429 | 70 | 6 | 0 | 130 | 199 | 1 | 70 | 7.96e-42 | 135 |
| MsaG026540 | MtrunA17_Chr5g0414001 | 72.840 | 81 | 17 | 2 | 1 | 81 | 207 | 282 | 2.69e-32 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026540 | AT1G24040.3 | 63.158 | 190 | 66 | 3 | 1 | 187 | 114 | 302 | 3.12e-82 | 247 |
| MsaG026540 | AT1G24040.2 | 63.158 | 190 | 66 | 3 | 1 | 187 | 114 | 302 | 3.12e-82 | 247 |
| MsaG026540 | AT1G24040.1 | 63.158 | 190 | 66 | 3 | 1 | 187 | 114 | 302 | 3.12e-82 | 247 |
Find 32 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGCCTTATATTTGTAATA+TGG | 0.284831 | 5:+23873988 | MsaT026540.1:CDS |
| ACAGCCATATTACAAATATA+AGG | 0.375127 | 5:-23873992 | None:intergenic |
| GGCTTATCCTTCAGCGGATC+CGG | 0.399495 | 5:-23873971 | None:intergenic |
| TCTCCCTTCAGGAGGGGCAT+TGG | 0.444220 | 5:+23878211 | MsaT026540.1:intron |
| TAATTCTACTCTTCCGTCGC+CGG | 0.460000 | 5:+23873952 | MsaT026540.1:CDS |
| GAGGAGTTTGATGCCGCATA+TGG | 0.462290 | 5:+23873823 | MsaT026540.1:CDS |
| CAAGCGAGGAACTGATTTCT+CGG | 0.465423 | 5:+23878251 | MsaT026540.1:CDS |
| TGCGTCTCTGCAACAACAGC+AGG | 0.469429 | 5:-23878384 | None:intergenic |
| CGCTATTGTATGCTTGCTTG+CGG | 0.471189 | 5:+23873718 | MsaT026540.1:CDS |
| ATTTCTCGGATGAGTTCATC+AGG | 0.474442 | 5:+23878265 | MsaT026540.1:CDS |
| AACACAGTAATATACCCTTT+CGG | 0.482167 | 5:-23873767 | None:intergenic |
| GATGAGGAAATGCAGTTGGC+TGG | 0.483939 | 5:+23873896 | MsaT026540.1:CDS |
| CTGTGGATAAATCTCTTAGA+AGG | 0.499813 | 5:+23874011 | MsaT026540.1:CDS |
| CCTTCAGGAGGGGCATTGGA+TGG | 0.505384 | 5:+23878215 | MsaT026540.1:intron |
| CATTGGATGGCATCTTCTAA+AGG | 0.517341 | 5:+23878228 | MsaT026540.1:CDS |
| TGAAACAGTATTTGATTGAA+CGG | 0.524045 | 5:+23873801 | MsaT026540.1:CDS |
| TTATATTTGTAATATGGCTG+TGG | 0.528854 | 5:+23873994 | MsaT026540.1:CDS |
| TCCTTCAGCGGATCCGGCGA+CGG | 0.532523 | 5:-23873965 | None:intergenic |
| ATGAGGAAATGCAGTTGGCT+GGG | 0.542657 | 5:+23873897 | MsaT026540.1:CDS |
| TCCGTCGCCGGATCCGCTGA+AGG | 0.545087 | 5:+23873964 | MsaT026540.1:CDS |
| AATCGATGATGTTTCCGAAA+GGG | 0.568903 | 5:+23873753 | MsaT026540.1:CDS |
| GGAAGTGGTGTTAATAGTGA+TGG | 0.582338 | 5:+23873869 | MsaT026540.1:CDS |
| GCAATCAAAGTCGCCATATG+CGG | 0.587969 | 5:-23873836 | None:intergenic |
| CATGGCAAATCTGTTTCTGA+AGG | 0.589946 | 5:-23878445 | None:intergenic |
| TACTCATAGTCATCAGAACA+TGG | 0.595102 | 5:-23878463 | None:intergenic |
| TCTGAAGGCATACTTATGAG+AGG | 0.618309 | 5:-23878430 | None:intergenic |
| GAATCGATGATGTTTCCGAA+AGG | 0.637311 | 5:+23873752 | MsaT026540.1:CDS |
| ATATAAGGCTTATCCTTCAG+CGG | 0.640663 | 5:-23873977 | None:intergenic |
| TAATAGTGATGGTGAAGATG+AGG | 0.648213 | 5:+23873880 | MsaT026540.1:CDS |
| TGAAGATGAGGAAATGCAGT+TGG | 0.653928 | 5:+23873892 | MsaT026540.1:CDS |
| GCATCTTCTAAAGGCAAGCG+AGG | 0.659909 | 5:+23878237 | MsaT026540.1:CDS |
| AACAGTATTTGATTGAACGG+AGG | 0.685549 | 5:+23873804 | MsaT026540.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 23873698 | 23878486 | 23873698 | ID=MsaG026540 |
| Chr5 | mRNA | 23873698 | 23878486 | 23873698 | ID=MsaT026540.1;Parent=MsaG026540 |
| Chr5 | exon | 23873698 | 23874032 | 23873698 | ID=MsaT026540.1.exon1;Parent=MsaT026540.1 |
| Chr5 | CDS | 23873698 | 23874032 | 23873698 | ID=cds.MsaT026540.1;Parent=MsaT026540.1 |
| Chr5 | exon | 23878222 | 23878486 | 23878222 | ID=MsaT026540.1.exon2;Parent=MsaT026540.1 |
| Chr5 | CDS | 23878222 | 23878486 | 23878222 | ID=cds.MsaT026540.1;Parent=MsaT026540.1 |
| Gene Sequence |
| Protein sequence |
