Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026677 | KEH15779.1 | 85.294 | 170 | 24 | 1 | 1 | 169 | 1 | 170 | 1.78e-97 | 303 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026677 | tr|A0A072TE13|A0A072TE13_MEDTR | 85.294 | 170 | 24 | 1 | 1 | 169 | 1 | 170 | 8.52e-98 | 303 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG026677 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026677 | MtrunA17_Chr1g0172971 | 82.051 | 117 | 20 | 1 | 1 | 116 | 1 | 117 | 1.25e-57 | 197 |
| MsaG026677 | MtrunA17_Chr3g0088371 | 54.913 | 173 | 39 | 5 | 269 | 405 | 15 | 184 | 6.59e-46 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 65 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTCGCTTCTTTGACCTAAT+TGG | 0.186128 | 5:-26686673 | None:intergenic |
| CTTTAGTCATTTCAACATTA+TGG | 0.194287 | 5:+26685804 | MsaT026677.1:CDS |
| TTCTTTGACCTAATTGGATT+TGG | 0.299426 | 5:-26686667 | None:intergenic |
| TTTGCTTCTTTCGAGCTATA+AGG | 0.313801 | 5:-26686735 | None:intergenic |
| TGTTCTTGAGGCAGGGAAAA+AGG | 0.322946 | 5:+26686519 | MsaT026677.1:CDS |
| GAGGGATAATCATGCTGATT+TGG | 0.346844 | 5:+26685837 | MsaT026677.1:CDS |
| GTCTTTAGTGCCAACATTCA+TGG | 0.367086 | 5:-26686393 | None:intergenic |
| CTCAACGGTGTTCTTGAGGC+AGG | 0.386173 | 5:+26686511 | MsaT026677.1:CDS |
| TCATGCTGATTTGGACTTCA+AGG | 0.389304 | 5:+26685846 | MsaT026677.1:CDS |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 5:-26684260 | None:intergenic |
| AAGAAAGACCAAATCCAATT+AGG | 0.397771 | 5:+26686659 | MsaT026677.1:CDS |
| TTTGGTCTTTCTTTGAACTT+TGG | 0.398856 | 5:-26686649 | None:intergenic |
| GAGGAGTTTCTTATTTCTAA+AGG | 0.403538 | 5:+26686544 | MsaT026677.1:CDS |
| CCATGATTCTTTATCACTAA+AGG | 0.406174 | 5:-26684021 | None:intergenic |
| TAAAGAATCATGGAAGTCTT+TGG | 0.408500 | 5:+26684031 | MsaT026677.1:CDS |
| GATTATGATCTTCAAACCTT+GGG | 0.413114 | 5:-26686149 | None:intergenic |
| ATAACACTGGATTCTCTGTT+AGG | 0.417808 | 5:+26684102 | MsaT026677.1:CDS |
| GGGTTGTCACAGTCGACATT+AGG | 0.419786 | 5:+26686184 | MsaT026677.1:CDS |
| CGATTATGATCTTCAAACCT+TGG | 0.425681 | 5:-26686150 | None:intergenic |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 5:+26684319 | MsaT026677.1:CDS |
| TGAATGTCTATAAGATATAT+AGG | 0.445219 | 5:+26684351 | MsaT026677.1:CDS |
| CGTTATGCTTGTAACAAAGA+AGG | 0.449439 | 5:+26684185 | MsaT026677.1:CDS |
| ATCTGAGAGTGGACAATTGA+CGG | 0.452529 | 5:+26683956 | MsaT026677.1:CDS |
| CTCCTCAAAATAGTATTTGA+AGG | 0.453936 | 5:-26685980 | None:intergenic |
| CTCAAGTAGCGCAAATGCTC+TGG | 0.460440 | 5:-26684407 | None:intergenic |
| GAAATGGTTGGTTGTAAAGC+TGG | 0.462514 | 5:+26684302 | MsaT026677.1:CDS |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 5:+26684151 | MsaT026677.1:CDS |
| TTTGAGGAGGACACAACAAA+AGG | 0.469432 | 5:+26685994 | MsaT026677.1:CDS |
| TTTAATTATGCATATAACAC+TGG | 0.485542 | 5:+26684089 | MsaT026677.1:CDS |
| CTGTCTGTGAACCATCTGTT+GGG | 0.487793 | 5:+26686620 | MsaT026677.1:CDS |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 5:+26684145 | MsaT026677.1:CDS |
| TTACAACCAACCATTTCCTC+TGG | 0.490470 | 5:-26684296 | None:intergenic |
| ACATTATGGTTCAGAGAATG+AGG | 0.494022 | 5:+26685818 | MsaT026677.1:CDS |
| GACCGCATAAGCTGACTGTT+GGG | 0.496307 | 5:-26685898 | None:intergenic |
| GCTGTCTGTGAACCATCTGT+TGG | 0.504047 | 5:+26686619 | MsaT026677.1:CDS |
| ATCATAATCGACTTGATGAA+AGG | 0.507955 | 5:+26686163 | MsaT026677.1:CDS |
| GATCTTCAAACCTTGGGGAA+AGG | 0.519004 | 5:-26686143 | None:intergenic |
| CAAAATAGTATTTGAAGGAA+AGG | 0.519540 | 5:-26685975 | None:intergenic |
| TAAAGAATCGAACAAGTGAA+TGG | 0.519618 | 5:-26685786 | None:intergenic |
| TGTCTATAAGATATATAGGT+GGG | 0.523740 | 5:+26684355 | MsaT026677.1:CDS |
| TCATAATCGACTTGATGAAA+GGG | 0.526860 | 5:+26686164 | MsaT026677.1:CDS |
| TTGAGGAGGACACAACAAAA+GGG | 0.529908 | 5:+26685995 | MsaT026677.1:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.538422 | 5:+26684196 | MsaT026677.1:CDS |
| AATCGAACAAGTGAATGGTC+TGG | 0.540971 | 5:-26685781 | None:intergenic |
| CCTTTAGTGATAAAGAATCA+TGG | 0.543522 | 5:+26684021 | MsaT026677.1:CDS |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 5:+26684197 | MsaT026677.1:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.552634 | 5:-26684395 | None:intergenic |
| TGAAAATTCAGTTATTGATG+CGG | 0.565546 | 5:+26683980 | MsaT026677.1:CDS |
| GGACCGCATAAGCTGACTGT+TGG | 0.579264 | 5:-26685899 | None:intergenic |
| TTTGGTGCAGTCCCAACAGA+TGG | 0.581566 | 5:-26686631 | None:intergenic |
| GAAGCAAAAGAAAGATGATG+AGG | 0.585686 | 5:+26686750 | MsaT026677.1:CDS |
| TGGTGTATACTTGTTTACTA+CGG | 0.585960 | 5:-26685920 | None:intergenic |
| TCAACGGTGTTCTTGAGGCA+GGG | 0.590097 | 5:+26686512 | MsaT026677.1:CDS |
| ATTTCTCAACGGTGTTCTTG+AGG | 0.591378 | 5:+26686507 | MsaT026677.1:CDS |
| TGAAGACTTAGAAGGCGTAA+CGG | 0.598405 | 5:-26686587 | None:intergenic |
| TTGAAGGAAACCTTTCCCCA+AGG | 0.600566 | 5:+26686133 | MsaT026677.1:CDS |
| TCGTGTTTGCGCAGATGCGG+AGG | 0.605841 | 5:+26686474 | MsaT026677.1:intron |
| ATCCCAACAGTCAGCTTATG+CGG | 0.606153 | 5:+26685896 | MsaT026677.1:CDS |
| ATATCAACTTCATCTGAGAG+TGG | 0.614980 | 5:+26683945 | MsaT026677.1:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 5:-26684245 | None:intergenic |
| ATTATGATCTTCAAACCTTG+GGG | 0.630358 | 5:-26686148 | None:intergenic |
| CATTATGGTTCAGAGAATGA+GGG | 0.643278 | 5:+26685819 | MsaT026677.1:CDS |
| ATGTCTATAAGATATATAGG+TGG | 0.643489 | 5:+26684354 | MsaT026677.1:CDS |
| TGAATAAATGAAAAGCAAGA+TGG | 0.655697 | 5:-26685940 | None:intergenic |
| GCATCTGAAATATTTCTCAA+CGG | 0.661104 | 5:+26686496 | MsaT026677.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 26683936 | 26686786 | 26683936 | ID=MsaG026677 |
| Chr5 | mRNA | 26683936 | 26686786 | 26683936 | ID=MsaT026677.1;Parent=MsaG026677 |
| Chr5 | exon | 26683936 | 26684442 | 26683936 | ID=MsaT026677.1.exon1;Parent=MsaT026677.1 |
| Chr5 | CDS | 26683936 | 26684442 | 26683936 | ID=cds.MsaT026677.1;Parent=MsaT026677.1 |
| Chr5 | exon | 26685781 | 26686041 | 26685781 | ID=MsaT026677.1.exon2;Parent=MsaT026677.1 |
| Chr5 | CDS | 26685781 | 26686041 | 26685781 | ID=cds.MsaT026677.1;Parent=MsaT026677.1 |
| Chr5 | exon | 26686130 | 26686231 | 26686130 | ID=MsaT026677.1.exon3;Parent=MsaT026677.1 |
| Chr5 | CDS | 26686130 | 26686231 | 26686130 | ID=cds.MsaT026677.1;Parent=MsaT026677.1 |
| Chr5 | exon | 26686346 | 26686400 | 26686346 | ID=MsaT026677.1.exon4;Parent=MsaT026677.1 |
| Chr5 | CDS | 26686346 | 26686400 | 26686346 | ID=cds.MsaT026677.1;Parent=MsaT026677.1 |
| Chr5 | exon | 26686488 | 26686786 | 26686488 | ID=MsaT026677.1.exon5;Parent=MsaT026677.1 |
| Chr5 | CDS | 26686488 | 26686786 | 26686488 | ID=cds.MsaT026677.1;Parent=MsaT026677.1 |
| Gene Sequence |
| Protein sequence |
