AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG026698


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026698 MtrunA17_Chr5g0413381 99.482 193 1 0 1 193 1 193 2.46e-144 400
MsaG026698 MtrunA17_Chr4g0063931 49.686 159 58 4 12 162 14 158 9.90e-45 147
MsaG026698 MtrunA17_Chr2g0322221 51.250 160 62 4 12 162 12 164 5.24e-44 145
MsaG026698 MtrunA17_Chr4g0025361 48.701 154 66 3 12 162 9 152 9.54e-41 137
MsaG026698 MtrunA17_Chr4g0011841 50.000 156 70 2 14 162 25 179 5.43e-40 136
MsaG026698 MtrunA17_Chr4g0056521 51.553 161 64 5 12 162 18 174 1.75e-39 135
MsaG026698 MtrunA17_Chr5g0419871 37.662 154 69 2 12 157 17 151 2.02e-27 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026698 AT1G23420.1 55.947 227 85 4 2 217 9 231 4.37e-73 221
MsaG026698 AT1G23420.2 55.947 227 85 4 2 217 40 262 5.81e-73 222
MsaG026698 AT2G26580.2 50.327 153 60 3 12 162 9 147 2.76e-46 151
MsaG026698 AT2G26580.1 50.327 153 60 3 12 162 9 147 2.76e-46 151
MsaG026698 AT2G45190.1 46.061 165 79 1 8 162 19 183 1.05e-42 144
MsaG026698 AT1G08465.1 49.677 155 66 4 12 162 8 154 7.65e-41 137
MsaG026698 AT4G00180.1 45.810 179 71 5 8 162 19 195 8.36e-39 134
MsaG026698 AT4G00180.2 43.976 166 67 5 21 162 1 164 6.60e-30 110
MsaG026698 AT1G69180.1 40.385 156 73 4 12 163 19 158 5.99e-29 107
MsaG026698 AT2G45190.2 63.636 66 24 0 97 162 54 119 4.09e-25 97.1

Find 43 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGCAGAGAAGAGATTAAA+AGG 0.220244 5:+26964758 MsaT026698.1:intron
CATCACCTTTGCACTGAGTT+GGG 0.247070 5:-26965023 None:intergenic
GCTTCCTTGTGAGCCATATC+AGG 0.254990 5:-26964796 None:intergenic
ACTCATGGTTACCATTAAAA+TGG 0.296363 5:-26964023 None:intergenic
CTTTCCAAGATGGAATTTCT+TGG 0.337983 5:-26965718 None:intergenic
GTCTTGTTTGCATCTTCATC+TGG 0.344790 5:-26964420 None:intergenic
GATGAGTAAGAGATGCTAAA+AGG 0.346967 5:-26964257 None:intergenic
GGTGTTCTTTGTCTCTTCTC+TGG 0.372695 5:-26964628 None:intergenic
TGTACATAGCAAATCTGTTC+TGG 0.376697 5:-26963983 None:intergenic
TCATCACCTTTGCACTGAGT+TGG 0.384038 5:-26965024 None:intergenic
TATTCATTCATTCACTCAAA+TGG 0.405002 5:-26965745 None:intergenic
TTATTGACTACATTGCTGAT+TGG 0.408933 5:-26964507 None:intergenic
CAGCTTCATGAGGGTCCACT+TGG 0.437038 5:-26965089 None:intergenic
ATCACCTTTGCACTGAGTTG+GGG 0.440172 5:-26965022 None:intergenic
GATCCACAAATTGATCTGTC+TGG 0.455209 5:-26965059 None:intergenic
ACAAGGATTCCGTGGAAGAA+AGG 0.512794 5:+26965693 MsaT026698.1:CDS
AGCATCTCTTACTCATCTTG+AGG 0.521370 5:+26964263 MsaT026698.1:CDS
GGTACCAAGAAATTCCATCT+TGG 0.522438 5:+26965714 MsaT026698.1:CDS
AGCTTCATGAGGGTCCACTT+GGG 0.522893 5:-26965088 None:intergenic
ACAATCAGAATAAGTCATCA+TGG 0.529221 5:-26964466 None:intergenic
AAATGGTGTCCTTTCCAAGA+TGG 0.529231 5:-26965728 None:intergenic
TAGCCAGACAGATCAATTTG+TGG 0.529249 5:+26965056 MsaT026698.1:CDS
AGAAGAGATTAAAAGGCTAA+AGG 0.529887 5:+26964765 MsaT026698.1:CDS
ATATTAATTACTTGATGAAG+CGG 0.533614 5:-26964663 None:intergenic
TTCTTGGTACCTTTCTTCCA+CGG 0.541076 5:-26965702 None:intergenic
CTTCCTTGTGAGCCATATCA+GGG 0.545406 5:-26964795 None:intergenic
GAGATGCTAAAAGGTGGAAA+GGG 0.553103 5:-26964248 None:intergenic
AGAGATGCTAAAAGGTGGAA+AGG 0.557063 5:-26964249 None:intergenic
GAGTAAGAGATGCTAAAAGG+TGG 0.562368 5:-26964254 None:intergenic
ACCATTGTCAAACTGCTGCA+TGG 0.563063 5:-26964159 None:intergenic
CAGGTGAATGAAGAAGAACA+AGG 0.564909 5:+26965676 MsaT026698.1:intron
GAAGAAGAACAAGGATTCCG+TGG 0.572480 5:+26965685 MsaT026698.1:CDS
AAACCCTGATATGGCTCACA+AGG 0.577441 5:+26964792 MsaT026698.1:CDS
CAAGAAATTCCATCTTGGAA+AGG 0.597804 5:+26965719 MsaT026698.1:CDS
TCACCTTTGCACTGAGTTGG+GGG 0.601505 5:-26965021 None:intergenic
GATGACTTATTCTGATTGTG+AGG 0.620697 5:+26964470 MsaT026698.1:CDS
CAGATTTGCTATGTACAATG+TGG 0.632418 5:+26963989 MsaT026698.1:CDS
AGTGGACCCTCATGAAGCTG+AGG 0.650640 5:+26965092 MsaT026698.1:CDS
TCCATGCAGCAGTTTGACAA+TGG 0.655787 5:+26964158 MsaT026698.1:CDS
ATGAAGCGGTTATAAGCTGA+TGG 0.663979 5:-26964649 None:intergenic
TGTGGATCTTGACTCCCAAG+TGG 0.667148 5:+26965074 MsaT026698.1:CDS
ATGCAGCAGTTTGACAATGG+TGG 0.669140 5:+26964161 MsaT026698.1:CDS
ATGGTGGTGACAGTAAGATG+TGG 0.764759 5:+26964177 MsaT026698.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 26963953 26965756 26963953 ID=MsaG026698
Chr5 mRNA 26963953 26965756 26963953 ID=MsaT026698.1;Parent=MsaG026698
Chr5 exon 26963953 26964033 26963953 ID=MsaT026698.1.exon1;Parent=MsaT026698.1
Chr5 CDS 26963953 26964033 26963953 ID=cds.MsaT026698.1;Parent=MsaT026698.1
Chr5 exon 26964150 26964284 26964150 ID=MsaT026698.1.exon2;Parent=MsaT026698.1
Chr5 CDS 26964150 26964284 26964150 ID=cds.MsaT026698.1;Parent=MsaT026698.1
Chr5 exon 26964402 26964531 26964402 ID=MsaT026698.1.exon3;Parent=MsaT026698.1
Chr5 CDS 26964402 26964531 26964402 ID=cds.MsaT026698.1;Parent=MsaT026698.1
Chr5 exon 26964626 26964674 26964626 ID=MsaT026698.1.exon4;Parent=MsaT026698.1
Chr5 CDS 26964626 26964674 26964626 ID=cds.MsaT026698.1;Parent=MsaT026698.1
Chr5 exon 26964765 26964840 26964765 ID=MsaT026698.1.exon5;Parent=MsaT026698.1
Chr5 CDS 26964765 26964840 26964765 ID=cds.MsaT026698.1;Parent=MsaT026698.1
Chr5 exon 26965009 26965113 26965009 ID=MsaT026698.1.exon6;Parent=MsaT026698.1
Chr5 CDS 26965009 26965113 26965009 ID=cds.MsaT026698.1;Parent=MsaT026698.1
Chr5 exon 26965679 26965756 26965679 ID=MsaT026698.1.exon7;Parent=MsaT026698.1
Chr5 CDS 26965679 26965756 26965679 ID=cds.MsaT026698.1;Parent=MsaT026698.1
Gene Sequence

>MsaT026698.1

ATGTCAACACTAAACCATCTTTTTGATCTTCCAGAACAGATTTGCTATGTACAATGTGGATTCTGCAATACCATTTTAATGGTAAGTGTTCCATGCAGCAGTTTGACAATGGTGGTGACAGTAAGATGTGGTCACTGCACAAGTCTCTTATCTGTTAACATGATGAAAGCTTCTTTTGTCCCTTTCCACCTTTTAGCATCTCTTACTCATCTTGAGCAAAAAGAAAGTAGTAGTCCAGATGAAGATGCAAACAAGACTTTGAACAGTAATACTAGTGCATCCATGATGACTTATTCTGATTGTGAGGAAGATGATGTAATTCCAATCAGCAATGTAGTCAATAAACCACCAGAGAAGAGACAAAGAACACCATCAGCTTATAACCGCTTCATCAAAGAAGAGATTAAAAGGCTAAAGGCTAAAAACCCTGATATGGCTCACAAGGAAGCTTTTAGTACAGCTGCAAAAAATTGGGCTAATTGTCCCCCAACTCAGTGCAAAGGTGATGAAGAGAGCTGTAGCCAGACAGATCAATTTGTGGATCTTGACTCCCAAGTGGACCCTCATGAAGCTGAGGTGAATGAAGAAGAACAAGGATTCCGTGGAAGAAAGGTACCAAGAAATTCCATCTTGGAAAGGACACCATTTGAGTGA

Protein sequence

>MsaT026698.1

MSTLNHLFDLPEQICYVQCGFCNTILMVSVPCSSLTMVVTVRCGHCTSLLSVNMMKASFVPFHLLASLTHLEQKESSSPDEDANKTLNSNTSASMMTYSDCEEDDVIPISNVVNKPPEKRQRTPSAYNRFIKEEIKRLKAKNPDMAHKEAFSTAAKNWANCPPTQCKGDEESCSQTDQFVDLDSQVDPHEAEVNEEEQGFRGRKVPRNSILERTPFE*