AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG026715

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026715 MtrunA17_Chr5g0414931 94.215 121 2 1 1 116 1 121 4.65e-76 225
MsaG026715 MtrunA17_Chr1g0170741 82.353 119 17 2 1 119 1 115 4.13e-63 191
MsaG026715 MtrunA17_Chr4g0059571 69.748 119 32 1 1 119 16 130 1.31e-48 154
MsaG026715 MtrunA17_Chr3g0118211 70.755 106 27 1 10 115 22 123 1.04e-45 147
MsaG026715 MtrunA17_Chr7g0256551 63.478 115 37 2 10 119 8 122 1.74e-44 145
MsaG026715 MtrunA17_Chr3g0082861 57.391 115 44 1 10 119 3 117 1.02e-40 133
MsaG026715 MtrunA17_Chr8g0352871 57.391 115 45 1 9 119 7 121 7.83e-40 131
MsaG026715 MtrunA17_Chr3g0128691 59.259 108 36 3 12 116 19 121 3.16e-39 129
MsaG026715 MtrunA17_Chr3g0124861 47.656 128 52 3 1 114 12 138 5.63e-33 115
MsaG026715 MtrunA17_Chr4g0021731 61.905 84 26 3 10 92 10 88 1.78e-26 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026715 AT1G10470.3 79.439 107 18 1 10 116 19 121 8.39e-52 164
MsaG026715 AT1G10470.1 79.439 107 18 1 10 116 33 135 1.26e-51 164
MsaG026715 AT1G59940.1 69.748 119 32 1 1 119 23 137 7.56e-51 161
MsaG026715 AT1G59940.2 69.748 119 32 1 1 119 115 229 6.17e-50 162
MsaG026715 AT3G48100.1 68.067 119 34 3 2 119 15 130 5.96e-49 155
MsaG026715 AT5G62920.1 71.818 110 25 2 11 119 25 129 7.54e-48 152
MsaG026715 AT1G19050.1 69.091 110 30 1 5 114 18 123 7.20e-47 150
MsaG026715 AT3G56380.1 60.714 112 41 1 5 116 14 122 1.46e-46 148
MsaG026715 AT1G74890.1 69.159 107 29 1 10 116 17 119 1.07e-45 147
MsaG026715 AT3G56380.2 60.177 113 41 2 5 116 14 123 8.79e-45 143
MsaG026715 AT2G40670.1 62.385 109 38 2 9 116 27 133 1.65e-44 143
MsaG026715 AT3G57040.2 61.207 116 39 1 10 119 8 123 3.46e-44 144
MsaG026715 AT3G57040.1 61.207 116 39 1 10 119 8 123 3.46e-44 144
MsaG026715 AT2G41310.1 60.976 123 40 3 1 119 3 121 3.74e-43 141
MsaG026715 AT2G40670.2 61.818 110 38 3 9 116 27 134 1.20e-42 138
MsaG026715 AT5G07210.2 35.354 99 49 2 10 108 15 98 4.04e-11 59.3

Find 22 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAAGAGCACTACAATTTC+TGG 0.202396 5:+27202886 MsaT026715.1:CDS
TTCCCAAGAGGTTCATGTTT+TGG 0.272257 5:+27202622 MsaT026715.1:CDS
GTTTCCTTAGCCTGGTTTGA+AGG 0.364670 5:+27203035 MsaT026715.1:intron
CTCAAAATTTCATCTTGTAA+AGG 0.415529 5:+27202689 MsaT026715.1:CDS
AACTCATAACCAGTCATTCC+TGG 0.425647 5:-27203087 None:intergenic
AGCACTACAATTTCTGGGGT+TGG 0.454081 5:+27202892 MsaT026715.1:CDS
ATAAGAGCACTACAATTTCT+GGG 0.455946 5:+27202887 MsaT026715.1:CDS
TGAGTTGCTTAAGAAAATCA+AGG 0.460054 5:+27203104 MsaT026715.1:CDS
ATTACAGATTATTGCATGCC+AGG 0.468259 5:+27203069 MsaT026715.1:CDS
CAGCCAAAACATGAACCTCT+TGG 0.469602 5:-27202625 None:intergenic
TATTGCATGCCAGGAATGAC+TGG 0.472610 5:+27203078 MsaT026715.1:CDS
TCAGAAGACATGATCACTAC+TGG 0.477520 5:-27203249 None:intergenic
TTAGTTACTGCAGTGGATAG+TGG 0.477996 5:+27202863 MsaT026715.1:intron
ACATCTTGCCACGCATTGAC+AGG 0.484895 5:+27203274 MsaT026715.1:CDS
AGCCAAAACATGAACCTCTT+GGG 0.497488 5:-27202624 None:intergenic
TCCTTAGCCTGGTTTGAAGG+TGG 0.500088 5:+27203038 MsaT026715.1:intron
TACTGGAATTGCTCTAAAAG+TGG 0.506655 5:-27203232 None:intergenic
TTGATCGTAAAGTCATTGAA+AGG 0.541223 5:+27202663 MsaT026715.1:CDS
ACTAGATCCACCTTCAAACC+AGG 0.592739 5:-27203045 None:intergenic
TACTAGTACCTGTCAATGCG+TGG 0.604768 5:-27203282 None:intergenic
AAACAGTGTTGTTTCCCAAG+AGG 0.619934 5:+27202610 MsaT026715.1:CDS
TAAGAGCACTACAATTTCTG+GGG 0.651134 5:+27202888 MsaT026715.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 27202602 27204300 27202602 ID=MsaG026715
Chr5 mRNA 27202602 27204300 27202602 ID=MsaT026715.1;Parent=MsaG026715
Chr5 exon 27202602 27202710 27202602 ID=MsaT026715.1.exon1;Parent=MsaT026715.1
Chr5 CDS 27202602 27202710 27202602 ID=cds.MsaT026715.1;Parent=MsaT026715.1
Chr5 exon 27202867 27202952 27202867 ID=MsaT026715.1.exon2;Parent=MsaT026715.1
Chr5 CDS 27202867 27202952 27202867 ID=cds.MsaT026715.1;Parent=MsaT026715.1
Chr5 exon 27203045 27203125 27203045 ID=MsaT026715.1.exon3;Parent=MsaT026715.1
Chr5 CDS 27203045 27203125 27203045 ID=cds.MsaT026715.1;Parent=MsaT026715.1
Chr5 exon 27203225 27203295 27203225 ID=MsaT026715.1.exon4;Parent=MsaT026715.1
Chr5 CDS 27203225 27203295 27203225 ID=cds.MsaT026715.1;Parent=MsaT026715.1
Chr5 exon 27204258 27204300 27204258 ID=MsaT026715.1.exon5;Parent=MsaT026715.1
Chr5 CDS 27204258 27204300 27204258 ID=cds.MsaT026715.1;Parent=MsaT026715.1
Gene Sequence

>MsaT026715.1

ATGGAGACAAACAGTGTTGTTTCCCAAGAGGTTCATGTTTTGGCTGTTGATGATAGTCTTGTTGATCGTAAAGTCATTGAAAGGTTGCTCAAAATTTCATCTTGTAAAGTTACTGCAGTGGATAGTGGAATAAGAGCACTACAATTTCTGGGGTTGGTTGATGAGAAAGAGACTTCTGAATCTGATGCTTTTGTTCCTGGTTTGAAGGTGGATCTAGTAATTACAGATTATTGCATGCCAGGAATGACTGGTTATGAGTTGCTTAAGAAAATCAAGGAATCATCCACTTTTAGAGCAATTCCAGTAGTGATCATGTCTTCTGAAAACATCTTGCCACGCATTGACAGGGGTTTTGATTGGTTTGATGGTTTTTGGAGGGGTTTATGGTGA
Protein sequence

>MsaT026715.1

METNSVVSQEVHVLAVDDSLVDRKVIERLLKISSCKVTAVDSGIRALQFLGLVDEKETSESDAFVPGLKVDLVITDYCMPGMTGYELLKKIKESSTFRAIPVVIMSSENILPRIDRGFDWFDGFWRGLW*