AlfalfaGEDB | MsaG026725

M. sativa ssp. caerulea / MsaG026725

KEGG: K14492
GO: GO:0000003 GO:0000156 GO:0000160 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009987 GO:0010017 GO:0010033 GO:0010114 GO:0010154 GO:0010161 GO:0010468 GO:0010556 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0023052 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048316 GO:0048511 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071214 GO:0071310 GO:0071368 GO:0071478 GO:0071482 GO:0071489 GO:0071491 GO:0071495 GO:0071704 GO:0080090 GO:0104004 GO:0140096 GO:1901564 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00072.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026725	XP_003613423.1	91.139	237	13	2	1	229	1	237	2.11e-124	362

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026725	sp\|Q9ZWS9\|ARR3_ARATH	52.155	232	72	6	1	221	23	226	7.77e-64	201

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026725	tr\|G7K5L8\|G7K5L8_MEDTR	91.139	237	13	2	1	229	1	237	1.01e-124	362

TFs/TRs:

Gene ID	Type	Classification
MsaG026725	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026725	MtrunA17_Chr5g0414931	91.139	237	13	2	1	229	1	237	1.94e-128	362
MsaG026725	MtrunA17_Chr1g0170741	65.948	232	42	5	1	229	1	198	3.53e-81	241
MsaG026725	MtrunA17_Chr4g0059571	66.447	152	44	2	1	149	16	163	3.37e-59	186
MsaG026725	MtrunA17_Chr3g0118211	65.693	137	40	2	10	143	22	154	1.16e-54	174
MsaG026725	MtrunA17_Chr7g0256551	58.278	151	55	3	10	152	8	158	1.32e-54	175
MsaG026725	MtrunA17_Chr8g0352871	56.081	148	56	3	10	149	8	154	2.57e-48	157
MsaG026725	MtrunA17_Chr3g0082861	54.861	144	57	2	10	145	3	146	3.60e-48	156
MsaG026725	MtrunA17_Chr3g0128691	57.463	134	46	4	12	139	19	147	3.82e-45	148
MsaG026725	MtrunA17_Chr3g0124861	46.795	156	65	4	1	139	12	166	1.04e-41	141
MsaG026725	MtrunA17_Chr4g0021731	61.905	84	26	3	10	92	10	88	6.41e-26	96.7
MsaG026725	MtrunA17_Chr4g0030271	29.661	236	132	5	11	229	89	307	8.96e-18	81.6
MsaG026725	MtrunA17_Chr3g0127941	28.497	193	109	5	11	187	56	235	3.70e-15	73.9
MsaG026725	MtrunA17_Chr1g0181811	28.125	192	108	5	13	187	87	265	7.81e-13	67.4
MsaG026725	MtrunA17_Chr8g0345901	30.216	139	81	2	9	144	34	159	8.46e-13	67.0
MsaG026725	MtrunA17_Chr7g0276361	29.630	135	79	3	13	144	16	137	4.67e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026725	AT1G59940.1	52.155	232	72	6	1	221	23	226	7.90e-65	201
MsaG026725	AT1G59940.2	52.155	232	72	6	1	221	115	318	1.22e-63	201
MsaG026725	AT1G10470.3	52.800	250	55	8	10	225	19	239	8.37e-63	196
MsaG026725	AT1G10470.1	52.800	250	55	8	10	225	33	253	1.37e-62	196
MsaG026725	AT3G48100.1	68.345	139	38	3	11	146	25	160	4.57e-58	182
MsaG026725	AT5G62920.1	72.308	130	27	3	11	136	25	149	1.50e-56	178
MsaG026725	AT1G19050.1	64.085	142	44	2	5	143	18	155	3.96e-55	175
MsaG026725	AT1G74890.1	67.692	130	35	2	10	136	17	142	7.26e-53	169
MsaG026725	AT3G57040.2	54.878	164	58	4	10	159	8	169	1.87e-52	169
MsaG026725	AT3G57040.1	54.878	164	58	4	10	159	8	169	1.87e-52	169
MsaG026725	AT2G41310.1	57.051	156	56	4	1	149	3	154	1.55e-51	167
MsaG026725	AT3G56380.1	57.246	138	53	2	5	139	14	148	5.17e-51	163
MsaG026725	AT3G56380.2	56.835	139	53	3	5	139	14	149	3.36e-49	158
MsaG026725	AT2G40670.1	57.778	135	51	3	9	139	27	159	3.49e-49	159
MsaG026725	AT2G40670.2	57.353	136	51	4	9	139	27	160	2.25e-47	154
MsaG026725	AT1G10470.2	39.869	153	63	5	92	225	35	177	6.83e-15	70.9
MsaG026725	AT5G24470.1	31.206	141	79	4	7	144	48	173	3.16e-13	68.6
MsaG026725	AT5G60100.7	35.338	133	70	3	13	142	66	185	4.98e-13	67.8
MsaG026725	AT5G60100.6	35.338	133	70	3	13	142	66	185	4.98e-13	67.8
MsaG026725	AT5G60100.4	35.338	133	70	3	13	142	66	185	4.98e-13	67.8
MsaG026725	AT5G60100.5	35.338	133	70	3	13	142	66	185	7.29e-13	67.8
MsaG026725	AT5G60100.3	35.338	133	70	3	13	142	66	185	7.29e-13	67.8
MsaG026725	AT5G60100.1	35.338	133	70	3	13	142	66	185	7.29e-13	67.8

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATAAGAGCACTACAATTTC+TGG	0.202396	5:+27395496	MsaT026725.1:CDS
TTCCCAAGAGGTTCATGTTT+TGG	0.272257	5:+27395232	MsaT026725.1:CDS
GTTTCCTTAGCCTGGTTTGA+AGG	0.364670	5:+27395645	MsaT026725.1:intron
CTCAAAATTTCATCTTGTAA+AGG	0.415529	5:+27395299	MsaT026725.1:CDS
AACTCATAACCAGTCATTCC+TGG	0.425647	5:-27395697	None:intergenic
TAGCGATAACTTCACTGTCT+TGG	0.452797	5:-27400765	None:intergenic
AGCACTACAATTTCTGGGGT+TGG	0.454081	5:+27395502	MsaT026725.1:CDS
ATAAGAGCACTACAATTTCT+GGG	0.455946	5:+27395497	MsaT026725.1:CDS
TGAAAGTGTTGATGATGAAA+TGG	0.456331	5:-27400890	None:intergenic
TGAGTTGCTTAAGAAAATCA+AGG	0.460054	5:+27395714	MsaT026725.1:CDS
ATTACAGATTATTGCATGCC+AGG	0.468259	5:+27395679	MsaT026725.1:CDS
CAGCCAAAACATGAACCTCT+TGG	0.469602	5:-27395235	None:intergenic
TATTGCATGCCAGGAATGAC+TGG	0.472610	5:+27395688	MsaT026725.1:CDS
TCAGAAGACATGATCACTAC+TGG	0.477520	5:-27395859	None:intergenic
TTAGTTACTGCAGTGGATAG+TGG	0.477996	5:+27395473	MsaT026725.1:intron
GGATAGGGATCAACAACAAA+AGG	0.483740	5:+27400827	MsaT026725.1:CDS
GATGGTGATGGTGTAGGGAT+AGG	0.492736	5:+27400811	MsaT026725.1:CDS
AGCCAAAACATGAACCTCTT+GGG	0.497488	5:-27395234	None:intergenic
TCCTTAGCCTGGTTTGAAGG+TGG	0.500088	5:+27395648	MsaT026725.1:intron
GCTTCCCGCTCTAACCTCCC+TGG	0.501953	5:-27400743	None:intergenic
TACTGGAATTGCTCTAAAAG+TGG	0.506655	5:-27395842	None:intergenic
GACAACCAGGGAGGTTAGAG+CGG	0.506833	5:+27400738	MsaT026725.1:CDS
ATGGTGATGGTGTAGGGATA+GGG	0.509422	5:+27400812	MsaT026725.1:CDS
ATTGGAGAATCAAGAATGTC+GGG	0.519018	5:-27400958	None:intergenic
ATCTTGCGTGAAACGTTTGA+AGG	0.520053	5:+27400708	MsaT026725.1:CDS
TCCAAAGAGTCATTCGGTGC+CGG	0.528910	5:-27400998	None:intergenic
TTCTTGATTCTCCAATCAGA+CGG	0.540193	5:+27400965	MsaT026725.1:CDS
TTGATCGTAAAGTCATTGAA+AGG	0.541223	5:+27395273	MsaT026725.1:CDS
ATAACGATGGTGATGGTGTA+GGG	0.541250	5:+27400806	MsaT026725.1:CDS
TGACAATCCAAAGAGTCATT+CGG	0.543587	5:-27401004	None:intergenic
GATAACGATGGTGATGGTGT+AGG	0.548802	5:+27400805	MsaT026725.1:CDS
ATCAGACGGCTTAAAATGAC+CGG	0.557664	5:+27400979	MsaT026725.1:CDS
TTCACGCAAGATAATTTCAC+CGG	0.562688	5:-27400697	None:intergenic
GATTGGAGAATCAAGAATGT+CGG	0.564103	5:-27400959	None:intergenic
AAGAATGTCGGGTGAAGACA+AGG	0.567906	5:-27400947	None:intergenic
AGAGGATTTCATAGTGAAGC+CGG	0.581417	5:+27400678	MsaT026725.1:CDS
TCTTGCGTGAAACGTTTGAA+GGG	0.583404	5:+27400709	MsaT026725.1:CDS
ACTAGATCCACCTTCAAACC+AGG	0.592739	5:-27395655	None:intergenic
GAAGACAAGGGTGATGATGA+AGG	0.594875	5:-27400934	None:intergenic
ACAACCAGGGAGGTTAGAGC+GGG	0.595105	5:+27400739	MsaT026725.1:CDS
TGAAGGGTTACATGACAACC+AGG	0.599838	5:+27400725	MsaT026725.1:CDS
GGTACATACAACAAAAGCAT+CGG	0.608957	5:-27395549	None:intergenic
GTTATCGCTACCAACGGTGT+TGG	0.609134	5:+27400778	MsaT026725.1:CDS
AAATGTAGATGCTTGGAGGA+AGG	0.613553	5:+27400652	MsaT026725.1:intron
AAACAGTGTTGTTTCCCAAG+AGG	0.619934	5:+27395220	MsaT026725.1:CDS
GTTATCGTTACCAACACCGT+TGG	0.634811	5:-27400788	None:intergenic
GAAGGTGACAGTGATGATGA+TGG	0.636333	5:-27400916	None:intergenic
ATGCTTGGAGGAAGGTGCAG+AGG	0.640084	5:+27400660	MsaT026725.1:CDS
TAAGAGCACTACAATTTCTG+GGG	0.651134	5:+27395498	MsaT026725.1:CDS
AGTGTTGATGATGAAATGGA+TGG	0.658494	5:-27400886	None:intergenic
AGTGAAGTTATCGCTACCAA+CGG	0.690720	5:+27400772	MsaT026725.1:CDS
GGTAACGATAACGATGGTGA+TGG	0.701040	5:+27400799	MsaT026725.1:CDS
GAAGGGTTACATGACAACCA+GGG	0.738936	5:+27400726	MsaT026725.1:CDS
AGAATGTCGGGTGAAGACAA+GGG	0.739456	5:-27400946	None:intergenic
GGTGTTGGTAACGATAACGA+TGG	0.744946	5:+27400793	MsaT026725.1:CDS
GGGTTACATGACAACCAGGG+AGG	0.751767	5:+27400729	MsaT026725.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	27395212	27401011	27395212	ID=MsaG026725
Chr5	mRNA	27395212	27401011	27395212	ID=MsaT026725.1;Parent=MsaG026725
Chr5	exon	27395212	27395320	27395212	ID=MsaT026725.1.exon1;Parent=MsaT026725.1
Chr5	CDS	27395212	27395320	27395212	ID=cds.MsaT026725.1;Parent=MsaT026725.1
Chr5	exon	27395477	27395562	27395477	ID=MsaT026725.1.exon2;Parent=MsaT026725.1
Chr5	CDS	27395477	27395562	27395477	ID=cds.MsaT026725.1;Parent=MsaT026725.1
Chr5	exon	27395655	27395735	27395655	ID=MsaT026725.1.exon3;Parent=MsaT026725.1
Chr5	CDS	27395655	27395735	27395655	ID=cds.MsaT026725.1;Parent=MsaT026725.1
Chr5	exon	27395835	27395896	27395835	ID=MsaT026725.1.exon4;Parent=MsaT026725.1
Chr5	CDS	27395835	27395896	27395835	ID=cds.MsaT026725.1;Parent=MsaT026725.1
Chr5	exon	27400660	27401011	27400660	ID=MsaT026725.1.exon5;Parent=MsaT026725.1
Chr5	CDS	27400660	27401011	27400660	ID=cds.MsaT026725.1;Parent=MsaT026725.1

Gene Sequence

>MsaT026725.1

ATGGAGACAAACAGTGTTGTTTCCCAAGAGGTTCATGTTTTGGCTGTTGATGATAGTCTTGTTGATCGTAAAGTCATTGAAAGGTTGCTCAAAATTTCATCTTGTAAAGTTACTGCAGTGGATAGTGGAATAAGAGCACTACAATTTCTGGGGTTGGTTGATGAGAAAGAGACTTCTGAATCCGATGCTTTTGTTCCTGGTTTGAAGGTGGATCTAGTAATTACAGATTATTGCATGCCAGGAATGACTGGTTATGAGTTGCTTAAGAAAATCAAGGAATCATCCACTTTTAGAGCAATTCCAGTAGTGATCATGTCTTCTGAAAACATCTTGCCACCATGCTTGGAGGAAGGTGCAGAGGATTTCATAGTGAAGCCGGTGAAATTATCTTGCGTGAAACGTTTGAAGGGTTACATGACAACCAGGGAGGTTAGAGCGGGAAGCCAAGACAGTGAAGTTATCGCTACCAACGGTGTTGGTAACGATAACGATGGTGATGGTGTAGGGATAGGGATCAACAACAAAAGGAAGCTAGATGAAGCATCTGATCTATCATCATCTGAACCATCCATTTCATCATCAACACTTTCATCACCATCATCATCACTGTCACCTTCATCATCACCCTTGTCTTCACCCGACATTCTTGATTCTCCAATCAGACGGCTTAAAATGACCGGCACCGAATGA

Protein sequence

>MsaT026725.1

METNSVVSQEVHVLAVDDSLVDRKVIERLLKISSCKVTAVDSGIRALQFLGLVDEKETSESDAFVPGLKVDLVITDYCMPGMTGYELLKKIKESSTFRAIPVVIMSSENILPPCLEEGAEDFIVKPVKLSCVKRLKGYMTTREVRAGSQDSEVIATNGVGNDNDGDGVGIGINNKRKLDEASDLSSSEPSISSSTLSSPSSSLSPSSSPLSSPDILDSPIRRLKMTGTE*